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https://www.readbyqxmd.com/read/28077807/folding-of-apomyoglobin-analysis-of-transient-intermediate-structure-during-refolding-using-quick-hydrogen-deuterium-exchange-and-nmr
#1
Chiaki Nishimura
The structures of apomyoglobin folding intermediates have been widely analyzed using physical chemistry methods including fluorescence, circular dichroism, small angle X-ray scattering, NMR, mass spectrometry, and rapid mixing. So far, at least two intermediates (on sub-millisecond- and millisecond-scales) have been demonstrated for apomyoglobin folding. The combination of pH-pulse labeling and NMR is a useful tool for analyzing the kinetic intermediates at the atomic level. Its use has revealed that the latter-phase kinetic intermediate of apomyoglobin (6 ms) was composed of helices A, B, G and H, whereas the equilibrium intermediate, called the pH 4 molten-globule intermediate, was composed mainly of helices A, G and H...
2017: Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
https://www.readbyqxmd.com/read/28067287/smartphone-based-low-light-detection-for-bioluminescence-application
#2
Huisung Kim, Youngkee Jung, Iyll-Joon Doh, Roxana Andrea Lozano-Mahecha, Bruce Applegate, Euiwon Bae
We report a smartphone-based device and associated imaging-processing algorithm to maximize the sensitivity of standard smartphone cameras, that can detect the presence of single-digit pW of radiant flux intensity. The proposed hardware and software, called bioluminescent-based analyte quantitation by smartphone (BAQS), provides an opportunity for onsite analysis and quantitation of luminescent signals from biological and non-biological sensing elements which emit photons in response to an analyte. A simple cradle that houses the smartphone, sample tube, and collection lens supports the measuring platform, while noise reduction by ensemble averaging simultaneously lowers the background and enhances the signal from emitted photons...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28067260/orientation-toward-humans-predicts-cognitive-performance-in-orang-utans
#3
Laura A Damerius, Sofia I F Forss, Zaida K Kosonen, Erik P Willems, Judith M Burkart, Josep Call, Birute M F Galdikas, Katja Liebal, Daniel B M Haun, Carel P van Schaik
Non-human animals sometimes show marked intraspecific variation in their cognitive abilities that may reflect variation in external inputs and experience during the developmental period. We examined variation in exploration and cognitive performance on a problem-solving task in a large sample of captive orang-utans (Pongo abelii &P. pygmaeus, N = 103) that had experienced different rearing and housing conditions during ontogeny, including human exposure. In addition to measuring exploration and cognitive performance, we also conducted a set of assays of the subjects' psychological orientation, including reactions towards an unfamiliar human, summarized in the human orientation index (HOI), and towards novel food and objects...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28052068/health-seeking-behaviour-towards-poverty-related-disease-prds-a-qualitative-study-of-people-living-in-camps-and-on-campuses-in-cameroon
#4
Valerie Makoge, Harro Maat, Lenneke Vaandrager, Maria Koelen
Poverty-Related Diseases (PRDs) emphasize poverty as a 'breeding-ground' for a range of diseases. The study presented here starts from the premise that poverty is a general condition that can limit people's capacity to prevent, mitigate or treat diseases. Using an interpretation of health seeking behaviour (HSB), inspired by the salutogenic approach, we investigated how people deal with PRDs, their ability and strategies put in place to cope. We collected HSB data from two groups of respondents in Cameroon: labourers of the Cameroon Development Corporation (CDC) living in settlements called camps and students of the state universities of Buea and Yaoundé living in settlements we refer to as campuses...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28048363/su-f-t-518-development-and-characterization-of-a-gated-treatment-system-implemented-with-an-in-house-optical-tracking-system-and-the-elekta-response-interface
#5
B Barraclough, J Park, F Li, B Lu, J Li, C Liu, G Yan
PURPOSE: To report the development and characterization of the first in-house gating system implemented with an optical tracking system (OTS) and the Elekta Response™ interface. METHODS: The Response™ connects a patient tracking system with a linac, enabling the tracking system to control radiation delivery. The developed system uses an in-house OTS to monitor patient breathing. The OTS consists of two infrared-based cameras, tracking markers affixed on patient...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28048072/su-f-t-259-gpr-tables-for-the-estimation-of-mid-plane-dose-using-epid
#6
Gopiraj Annamalai, Yoichi Watanabe
PURPOSE: To develop a simple method for estimating the mid-plane dose (MPD) of a patient using Electronic Portal imaging Device (EPID). METHODS: A Varian TrueBeam with aSi100 EPID was used in this study. The EPID images were acquired for a 30 cm × 30 cm homogeneous slab phantom and a 30 cm diameter 20 cm thick cylindrical phantom in the continuous dosimetry mode. The acquired EPID images in XIM format were imported into in-house MATLAB program for the data analysis...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28043544/development-of-an-indirect-elisa-system-to-diagnosis-paratuberculosis-using-surface-antigens-of-mycobacterium-avium-subsp-paratuberculosis-in-cattle
#7
Mona Hatamifar, Nader Mosavari, Javad Kazemi
BACKGROUND/OBJECTIVE: Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis (PTB), also called as Johne's disease, and is considered as the cause of irreparable economic losses in livestock industry. This bacterium is excreted in large amounts through the milk and feces of infected animal. For the detection of the PTB, indirect ELISA has been highly considered as a simple method with high sensitivity and specificity. Accordingly, this study aims at designing a system of indirect ELISA for the detection of PTB...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28042059/operationalizing-the-measuring-what-matters-spirituality-quality-metric-in-a-population-of-hospitalized-critically-ill-patients-and-their-family-members
#8
Rebecca A Aslakson, Josephine Kweku, Malonnie Kinnison, Sarabdeep Singh, Thomas Y Crowe
CONTEXT: Measuring What Matters (MWM) quality indicators support measurement of the percentage of patients who have spiritual discussions, if desired. OBJECTIVES: To 1) determine the ease of, and barriers to, prospectively collecting MWM spirituality quality measure data and 2) further explore the importance of spirituality in a seriously ill, hospitalized population of critically ill patients and their family members. METHODS: Electronic medical record (EMR) review and cross-sectional survey of ICU patients and their family members from October-December 2015...
December 29, 2016: Journal of Pain and Symptom Management
https://www.readbyqxmd.com/read/28029709/increasing-role-of-nurse-practitioners-in-house-call-programs
#9
Nengliang Aaron Yao, Karen Rose, Virginia LeBaron, Fabian Camacho, Peter Boling
OBJECTIVES: Residence-based primary care provides homebound frail patients with a care plan that is individually tailored to manage multiple chronic conditions and functional limitations using a variety of resources. We (1) examine the visit volume and Medicare payments for residence-based health care provided by nurse practitioners (NPs) in the Medicare fee-for-service environment; (2) compare NP's residential visits to those of internists and family physicians; and (3) compare the geographical service area of full-time house call NPs versus NPs who make nursing facility visits a major portion of their work...
December 28, 2016: Journal of the American Geriatrics Society
https://www.readbyqxmd.com/read/28029581/right-atrial-appendage-aneurysms-in-veal-calves-and-beef-cattle-anatomopathological-investigations-and-aetiopathogenetic-hypotheses
#10
I Biasato, E Biasibetti, S Gili, G Bruatto, A Tarducci, F Guarda, M T Capucchio
OBJECTIVES: Right atrial aneurysms have been reported in bovine species, but a clear aetiopathogenesis has never been elucidated. ANIMALS: One thousand and seventy-nine veal calves (6-9 months old) and 313 beef cattle (10-24 months old) housed in intensive livestock farming systems and regularly slaughtered were included in the present study. METHODS: Hearts were externally examined and the identified right atrial aneurysms were submitted for gross and histopathological investigations...
October 28, 2016: Journal of Veterinary Cardiology: the Official Journal of the European Society of Veterinary Cardiology
https://www.readbyqxmd.com/read/28028197/limited-cognitive-resources-explain-a-tradeoff-between-perceptual-and-metacognitive-vigilance
#11
Brian Maniscalco, Li Yan McCurdy, Brian Odegaard, Hakwan Lau
: Why do experimenters give subjects short breaks in long behavioral experiments? Whereas earlier studies suggest it is difficult to maintain attention and vigilance over long periods of time, it is unclear precisely what mechanisms benefit from rest after short experimental blocks. Here, we evaluate decline in both perceptual performance and metacognitive sensitivity (i.e., how well confidence ratings track perceptual decision accuracy) over time, and investigate whether characteristics of prefrontal cortical areas correlate with these measures...
December 27, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28024607/robust-formulation-for-the-design-of-tissue-engineering-scaffolds-a-comprehensive-study-on-structural-anisotropy-viscoelasticity-and-degradation-of-3d-scaffolds-fabricated-with-customized-desktop-robot-based-rapid-prototyping-drbrp-system
#12
M Enamul Hoque
This study investigates the scaffolds' structural anisotropy (i.e. the effect of loading direction), viscoelasticity (i.e. the effect of cross head speed or strain rate), and the influence of simulated physiological environment (PBS solution at 37°C) on the mechanical properties. Besides, the in vitro degradation study has also been performed that evaluates the effect of variation in material and lay-down pattern on the scaffolds' degradation kinetics in terms of mass loss, and change in morphological and mechanical properties...
March 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28009100/rare-copy-number-variants-in-a-population-based-investigation-of-hypoplastic-right-heart-syndrome
#13
Aggeliki Dimopoulos, Robert J Sicko, Denise M Kay, Shannon L Rigler, Charlotte M Druschel, Michele Caggana, Marilyn L Browne, Ruzong Fan, Paul A Romitti, Lawrence C Brody, James L Mills
BACKGROUND: Hypoplastic right heart syndrome (HRHS) is a rare congenital defect characterized by underdevelopment of the right heart structures commonly accompanied by an atrial septal defect. Familial HRHS reports suggest genetic factor involvement. We examined the role of copy number variants (CNVs) in HRHS. METHODS: We genotyped 32 HRHS cases identified from all New York State live births (1998-2005) using Illumina HumanOmni2.5 microarrays. CNVs were called with PennCNV and prioritized if they were ≥20 Kb, contained ≥10 SNPs and had minimal overlap with CNVs from in-house controls, the Database of Genomic Variants, HapMap3, and Childrens Hospital of Philadelphia database...
December 23, 2016: Birth Defects Research. Part A, Clinical and Molecular Teratology
https://www.readbyqxmd.com/read/27998406/-risk-behaviors-among-newly-reported-burmese-hiv-infection-in-dehong-dai-and-jingpo-autonomous-prefectures-of-yunnan-province-2015
#14
L Li, Y C Zhang, Y C Yang, Y F Cao, R H Tang, R H Ye, S J Yang, S T Yao, J B Wang, S Duan, N He
Objective: To study the AIDS-related socio-demographic characteristics and high risk behaviors of Burmese HIV-infected individuals, in Dehong prefecture, Yunnan province. Methods: A cross-sectional study with questionnaire interview, was conducted. Results: Features of the 489 HIV-infected individuals appearfed as: 367(75.1%) males, 407(83.2%) illiterate or with primary education, 371(75.9%) from Kachin State or Shan State, 306(62.6%) visited Dehong twice or more per year, 238(48.7%) entered Dehong illegally, 339(69...
December 10, 2016: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/27978425/moving-the-house-party-to-the-lab-a-call-for-experimental-studies-on-drinking-games
#15
Byron L Zamboanga, Philip K Peake
No abstract text is available yet for this article.
December 2, 2016: Addictive Behaviors
https://www.readbyqxmd.com/read/27977361/midwives-on-a-mission
#16
(no author information available yet)
[Figure: see text] The nurses of Nonnatus House are swapping a white Christmas in east London for a mission to South Africa in the Christmas special of Call the Midwife, pictured.
December 14, 2016: Nursing Standard
https://www.readbyqxmd.com/read/27968859/the-health-brain-drain-in-rural-areas-about-the-health-personnel-incentive-policy-intervention-in-shanghai-a-longitudinal-study
#17
Kun Liu, Limei Jing, Xuhong Zhou, Yao Huang, Li Wang, Xiaoming Sun
BACKGROUND: The so-called health brain-drain-which refers to the health workers leaving their present jobs to other institutions, especially in rural areas-severely restricts the development of primary healthcare. To solve the problem, Pudong New Area of Shanghai carried out a series of incentive policies such as salary bonus, promotion and housing from July, 2014. In our study we aimed to analyse the change in health brain-drain which resulted from this policy. METHODS: There are 45 community health centres in Pudong, which we classified into 4 categories by geographical division: remote rural, general rural, suburban, and urban...
October 2016: Lancet
https://www.readbyqxmd.com/read/27964746/alternate-locus-aware-variant-calling-in-whole-genome-sequencing
#18
Marten Jäger, Max Schubach, Tomasz Zemojtel, Knut Reinert, Deanna M Church, Peter N Robinson
BACKGROUND: The last two human genome assemblies have extended the previous linear golden-path paradigm of the human genome to a graph-like model to better represent regions with a high degree of structural variability. The new model offers opportunities to improve the technical validity of variant calling in whole-genome sequencing (WGS). METHODS: We developed an algorithm that analyzes the patterns of variant calls in the 178 structurally variable regions of the GRCh38 genome assembly, and infers whether a given sample is most likely to contain sequences from the primary assembly, an alternate locus, or their heterozygous combination at each of these 178 regions...
December 13, 2016: Genome Medicine
https://www.readbyqxmd.com/read/27942534/information-and-communication-technology-use-by-female-residents-of-public-housing
#19
Lisa M Quintiliani, Shivani Reddy, Rachel Goodman, Deborah J Bowen
BACKGROUND: Evidence suggests that Internet, mobile, or social media based-interventions may promote obesity-lowering behavior change, which has implications for cancer prevention and control interventions. However, the uptake of communication technologies among low socioeconomic status individuals, who need obesity management strategies most, is unclear. METHODS: Using the baseline data from a cluster-randomized behavioral intervention trial, we examined the cross-sectional associations of frequency of information and communication technologies (ICT) use among female public housing residents, as well as the variation of ICT use across demographic and health-related variables...
October 2016: MHealth
https://www.readbyqxmd.com/read/27936262/-a-mental-health-hygiene-experiment-the-case-of-les-pinceaux-d-or
#20
Hubert Wallot
Many research, notably that of Gene Cohen (2007), have shown positive impact of artistic activities for seniors. In 2006 in Quebec City, three professional painters have created a none lucrative organization called Les Pinceaux d'Or (The Golden Brushes). Its mission is to create a positive experience of painting learning in elderly needing persons. The organization operates in long-term health-care facilities or in low-income housings and day centers. It offers free painting courses to elderly persons referred by local community health centers or community organizations as seniors with psychosocial needs...
2016: Santé Mentale Au Québec
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