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https://www.readbyqxmd.com/read/28317026/toxoplasma-gondii-ap2ix-4-regulates-gene-expression-during-bradyzoite-development
#1
Sherri Huang, Michael J Holmes, Joshua B Radke, Dong-Pyo Hong, Ting-Kai Liu, Michael W White, William J Sullivan
Toxoplasma gondii is a protozoan parasite of great importance to human and animal health. In the host, this obligate intracellular parasite persists as a tissue cyst that is imperceptible to the immune response and unaffected by current therapies. The tissue cysts facilitate transmission through predation and give rise to chronic cycles of toxoplasmosis in immunocompromised patients. Transcriptional changes accompany conversion of the rapidly replicating tachyzoites into the encysted bradyzoites, and yet the mechanisms underlying these alterations in gene expression are not well defined...
March 2017: MSphere
https://www.readbyqxmd.com/read/28281999/the-hidden-story-of-innovation-charity-hospital-angola-prison-and-the-challenging-of-surgical-dogma
#2
Patrick Greiffenstein, Paul R Hastings
The late 1960s was a period of significant upheaval of social, cultural, and scientific norms. The generally accepted notion of mandatory laparotomy for all penetrating abdominal injuries was among those norms being called into question across the country and many advocated expectant management of selected patients presenting with this type of injury. Leaders of the surgical community published opinions on either side of the argument. The house staff at Charity Hospital during this period was among the busiest in the nation in treating these injuries, many of them inmates of the Louisiana State Penitentiary who used self-inflicted stab wounds to the abdomen as a means of temporary respite from the inhumane conditions in the prison...
February 1, 2017: American Surgeon
https://www.readbyqxmd.com/read/28278745/-ballroom-itself-can-either-make-you-or-break-you-black-gbt-youths-psychosocial-development-in-the-house-ball-community
#3
Kyle Telander, Sybil G Hosek, Diana Lemos, Gihane Jeremie-Brink
Social context plays a significant role in adolescent identity development, particularly for youth lacking traditional systems of support. Using ecological and symbolic interactionism perspectives, this study qualitatively explored the psychosocial identity development of Black gay, bisexual, or transgendered youth participating in the House Ball Community (HBC). The HBC is a diverse network of family-like structures called 'houses', as well as a glamorous social outlet via pageant-like 'balls' in which participants compete...
March 3, 2017: Global Public Health
https://www.readbyqxmd.com/read/28274221/effectiveness-of-a-community-paramedic-led-health-assessment-and-education-initiative-in-a-seniors-residence-building-the-community-health-assessment-program-through-emergency-medical-services-chap-ems
#4
G Agarwal, R Angeles, M Pirrie, F Marzanek, B McLeod, J Parascandalo, L Dolovich
BACKGROUND: Seniors living in subsidized housing have lower income, poorer health, and increased risk for cardiometabolic diseases and falls. Seniors also account for more than one third of calls to Emergency Medical Services (EMS). This study examines the effectiveness of the Community Health Assessment Program through EMS (CHAP-EMS) in reducing blood pressure, diabetes risk, and EMS calls. METHODS: Paramedics on modified duty (e.g. injured) conducted weekly, one-on-one drop-in sessions in a common area of one subsidized senior's apartment building in Hamilton, Ontario...
March 9, 2017: BMC Emergency Medicine
https://www.readbyqxmd.com/read/28244812/primary-care-providers-use-of-pharmacist-support-for-delivery-of-pharmacogenetic-testing
#5
Susanne B Haga, Rachel Mills, Jivan Moaddeb, Nancy Allen LaPointe, Alex Cho, Geoffrey S Ginsburg
AIM: To investigate provider utilization of pharmacist support in the delivery of pharmacogenetic testing in a primary care setting. METHODS: Two primary care clinics within Duke University Health System participated in the study between December 2012 and July 2013. One clinic was provided with an in-house pharmacist and the second clinic had an on-call pharmacist. RESULTS: Providers in the in-house pharmacist arm consulted with the pharmacist for 13 of 15 cases, or about one of every four patients tested compared with one of every 7...
March 2017: Pharmacogenomics
https://www.readbyqxmd.com/read/28228196/the-hidden-story-of-innovation-charity-hospital-angola-prison-and-the-challenging-of-surgical-dogma
#6
Patrick Greiffenstein, Paul R Hastings
The late 1960s was a period of significant upheaval of social, cultural, and scientific norms. The generally accepted notion of mandatory laparotomy for all penetrating abdominal injuries was among those norms being called into question across the country and many advocated expectant management of selected patients presenting with this type of injury. Leaders of the surgical community published opinions on either side of the argument. The house staff at Charity Hospital during this period was among the busiest in the nation in treating these injuries, many of them inmates of the Louisiana State Penitentiary who used self-inflicted stab wounds to the abdomen as a means of temporary respite from the inhumane conditions in the prison...
February 1, 2017: American Surgeon
https://www.readbyqxmd.com/read/28222838/prevalence-of-food-impaction-induced-periodontitis-in-conventionally-housed-marsh-rice-rats-oryzomys-palustris
#7
Jonathan G Messer, Jessica M Jiron, Hung-Yuan Chen, Evelyn J Castillo, Jorge L Mendieta Calle, Mary K Reinhard, Donald B Kimmel, J Ignacio Aguirre
Marsh rice rats (Oryzomys palustris) fed a pelleted diet high in sucrose and casein have been used as a model for moderate to severe periodontitis. Here we characterize the prevalence, location, and histopathologic features of food-impaction lesions (FIL), a unique type of oral event, in rice rats fed standard pelleted rodent chow from weaning until 34 wk of age. Healthy female rats (n = 90; age, 4 wk) were weaned into groups (n = 10 to 24) and were euthanized at 4, 16, 22, 28, or 34 wk of age. At necropsy, high-resolution photographs of the 4 jaw quadrants were examined by 3 independent observers to determine the presence, number, and location of FIL...
February 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28219736/development-of-minimal-enzyme-cocktails-for-hydrolysis-of-sulfite-pulped-lignocellulosic-biomass
#8
Piotr Chylenski, Zarah Forsberg, Jerry Ståhlberg, Anikó Várnai, Martin Lersch, Oskar Bengtsson, Solve Sæbø, Svein Jarle Horn, Vincent G H Eijsink
Despite recent progress, saccharification of lignocellulosic biomass is still a major cost driver in biorefining. In this study, we present the development of minimal enzyme cocktails for hydrolysis of Norway spruce and sugarcane bagasse, which were pretreated using the so-called BALI™ process, which is based on sulfite pulping technology. Minimal enzyme cocktails were composed using several glycoside hydrolases purified from the industrially relevant filamentous fungus Trichoderma reesei and a purified commercial β-glucosidase from Aspergillus niger...
March 20, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28210351/cross-continuum-tool-is-associated-with-reduced-utilization-and-cost-for-frequent-high-need-users
#9
Lauran Hardin, Adam Kilian, Leslie Muller, Kevin Callison, Michael Olgren
INTRODUCTION: High-need, high-cost (HNHC) patients can over-use acute care services, a pattern of behavior associated with many poor outcomes that disproportionately contributes to increased U.S. healthcare cost. Our objective was to reduce healthcare cost and improve outcomes by optimizing the system of care. We targeted HNHC patients and identified root causes of frequent healthcare utilization. We developed a cross-continuum intervention process and a succinct tool called a Complex Care Map (CCM)© that addresses fragmentation in the system and links providers to a comprehensive individualized analysis of the patient story and causes for frequent access to health services...
February 2017: Western Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28208246/combined-proteome-and-eicosanoid-profiling-approach-for-revealing-implications-of-human-fibroblasts-in-chronic-inflammation
#10
Ammar Tahir, Andrea Bileck, Besnik Muqaku, Laura Niederstaetter, Dominique Kreutz, Rupert L Mayer, Denise Wolrab, Samuel M Meier, Astrid Slany, Christopher Gerner
During inflammation, proteins and lipids act in a concerted fashion, calling for combined analyses. Fibroblasts are powerful mediators of chronic inflammation. However, little is known about eicosanoid formation by human fibroblasts. The aim of this study was to analyze the formation of the most relevant inflammation mediators including proteins and lipids in human fibroblasts upon inflammatory stimulation and subsequent treatment with dexamethasone, a powerful antiphlogistic drug. Label-free quantification was applied for proteome profiling, while an in-house established data-dependent analysis method based on high-resolution mass spectrometry was applied for eicosadomics...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28178742/-urgency-of-gp-emergency-visits-to-nursing-homes
#11
Markus Bleckwenn, Sabrina Ahrens, Rieke Schnakenberg, Klaus Weckbecker
 Until now there are no uniform criteria or standards for classification of emergency home visits. Due to demographic changes, especially urgent visits to patients in nursing homes will increase. In the URGENT study, physicians and nurses rated the urgency of emergency visits to nursing home patients before and after the visit. This should help to see how many emergency house calls had been classified as less urgent and how often the assessment of physicians and nurses were in agreement with each other. In a prospective observational study, home visits were analyzed, which were classified as urgent at request by the physicians...
February 8, 2017: Das Gesundheitswesen
https://www.readbyqxmd.com/read/28173807/harm-reduction-through-housing-first-an-assessment-of-the-emergency-warming-centre-in-inuvik-canada
#12
Michael G Young, Kathleen Manion
BACKGROUND: This research examines the effectiveness of an Emergency Warming Centre (EWC) in Inuvik, Canada, at reducing rates of morbidity and mortality for homeless persons with concurrent disorders (mental health problems and addictions). Inuvik is a small town of approximately 3500 residents, with over 65% being Aboriginal. The town is situated on the Beaufort Delta in the Western Canadian Arctic and is subject to oil and gas extraction-based boom and bust economic cycles. The centre provided food and accommodation for those under the influence of alcohol or drugs who had no other place to stay...
February 7, 2017: Harm Reduction Journal
https://www.readbyqxmd.com/read/28161556/morphogenesis-and-maturation-of-the-embryonic-and-postnatal-intestine
#13
REVIEW
Alana M Chin, David R Hill, Megan Aurora, Jason R Spence
The intestine is a vital organ responsible for nutrient absorption, bile and waste excretion, and a major site of host immunity. In order to keep up with daily demands, the intestine has evolved a mechanism to expand the absorptive surface area by undergoing a morphogenetic process to generate finger-like units called villi. These villi house specialized cell types critical for both absorbing nutrients from food, and for protecting the host from commensal and pathogenic microbes present in the adult gut. In this review, we will discuss mechanisms that coordinate intestinal development, growth, and maturation of the small intestine, starting from the formation of the early gut tube, through villus morphogenesis and into early postnatal life when the intestine must adapt to the acquisition of nutrients through food intake, and to interactions with microbes...
February 1, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28155670/esap-plus-a-web-based-server-for-est-ssr-marker-development
#14
Piyarat Ponyared, Jiradej Ponsawat, Sissades Tongsima, Pusadee Seresangtakul, Chutipong Akkasaeng, Nathpapat Tantisuwichwong
BACKGROUND: Simple sequence repeats (SSRs) have become widely used as molecular markers in plant genetic studies due to their abundance, high allelic variation at each locus and simplicity to analyze using conventional PCR amplification. To study plants with unknown genome sequence, SSR markers from Expressed Sequence Tags (ESTs), which can be obtained from the plant mRNA (converted to cDNA), must be utilized. With the advent of high-throughput sequencing technology, huge EST sequence data have been generated and are now accessible from many public databases...
December 22, 2016: BMC Genomics
https://www.readbyqxmd.com/read/28145002/cohabitants-perspective-on-housing-adaptations-a-piece-of-the-puzzle
#15
Marianne Granbom, Afsaneh Taei, Lisa Ekstam
As part of the Swedish state-funded healthcare system, housing adaptations are used to promote safe and independent living for disabled people in ordinary housing through the elimination of physical environmental barriers in the home. The aim of this study was to describe the cohabitants' expectations and experiences of how a housing adaptation, intended for the partner, would impact their everyday life. In-depth interviews were conducted with cohabitants of nine people applying for a housing adaptation, initially at the time of the application and then again 3 months after the housing adaptation was installed...
January 31, 2017: Scandinavian Journal of Caring Sciences
https://www.readbyqxmd.com/read/28143617/fast-and-accurate-mutation-detection-in-whole-genome-sequences-of-multiple-isogenic-samples-with-isomut
#16
O Pipek, D Ribli, J Molnár, Á Póti, M Krzystanek, A Bodor, G E Tusnády, Z Szallasi, I Csabai, D Szüts
BACKGROUND: Detection of somatic mutations is one of the main goals of next generation DNA sequencing. A wide range of experimental systems are available for the study of spontaneous or environmentally induced mutagenic processes. However, most of the routinely used mutation calling algorithms are not optimised for the simultaneous analysis of multiple samples, or for non-human experimental model systems with no reliable databases of common genetic variations. Most standard tools either require numerous in-house post filtering steps with scarce documentation or take an unpractically long time to run...
January 31, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28134765/dimerized-translationally-controlled-tumor-protein-binding-peptide-ameliorates-atopic-dermatitis-in-nc-nga-mice
#17
Xing-Hai Jin, Juhyeon Lim, Dong Hae Shin, Jeehye Maeng, Kyunglim Lee
Our previous study showed that dimerized translationally controlled tumor protein (dTCTP) plays a role in the pathogenesis of allergic diseases, such as asthma and allergic rhinitis. A 7-mer peptide, called dTCTP-binding peptide 2 (dTBP2), binds to dTCTP and inhibits its cytokine-like effects. We therefore examined the protective effects of dTBP2 in house dust mite-induced atopic dermatitis (AD)-like skin lesions in Nishiki-nezumi Cinnamon/Nagoya (NC/Nga) mice. We found that topical administration of dTBP2 significantly reduced the AD-like skin lesions formation and mast cell infiltration in NC/Nga mice, similarly to the response seen in the Protopic (tacrolimus)-treated group...
January 26, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28122122/the-effect-of-radiation-dose-reduction-on-computer-aided-detection-cad-performance-in-a-low-dose-lung-cancer-screening-population
#18
Stefano Young, Pechin Lo, Grace Kim, Matthew Brown, John Hoffman, William Hsu, Wasil Wahi-Anwar, Carlos Flores, Grace Lee, Frederic Noo, Jonathan Goldin, Michael McNitt-Gray
PURPOSE: Lung cancer screening with low-dose CT has recently been approved for reimbursement, heralding the arrival of such screening services worldwide. Computer-Aided Detection (CAD) tools offer the potential to assist radiologists in detecting nodules in these screening exams. In lung screening, as in all CT exams, there is interest in further reducing radiation dose. However, the effects of continued dose reduction on CAD performance are not fully understood. In this work, we investigated the effect of reducing radiation dose on CAD lung-nodule detection performance in a screening population...
January 25, 2017: Medical Physics
https://www.readbyqxmd.com/read/28117786/dissection-of-the-auditory-bulla-in-postnatal-mice-isolation-of-the-middle-ear-bones-and-histological-analysis
#19
Ayako Sakamoto, Yukiko Kuroda, Sho Kanzaki, Koichi Matsuo
In most mammals, auditory ossicles in the middle ear, including the malleus, incus and stapes, are the smallest bones. In mice, a bony structure called the auditory bulla houses the ossicles, whereas the auditory capsule encloses the inner ear, namely the cochlea and semicircular canals. Murine ossicles are essential for hearing and thus of great interest to researchers in the field of otolaryngology, but their metabolism, development, and evolution are highly relevant to other fields. Altered bone metabolism can affect hearing function in adult mice, and various gene-deficient mice show changes in morphogenesis of auditory ossicles in utero...
January 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28109296/design-of-simulation-based-medical-education-and-advantages-and-disadvantages-of-in-situ-simulation-versus-off-site-simulation
#20
EDITORIAL
Jette Led Sørensen, Doris Østergaard, Vicki LeBlanc, Bent Ottesen, Lars Konge, Peter Dieckmann, Cees Van der Vleuten
BACKGROUND: Simulation-based medical education (SBME) has traditionally been conducted as off-site simulation in simulation centres. Some hospital departments also provide off-site simulation using in-house training room(s) set up for simulation away from the clinical setting, and these activities are called in-house training. In-house training facilities can be part of hospital departments and resemble to some extent simulation centres but often have less technical equipment. In situ simulation, introduced over the past decade, mainly comprises of team-based activities and occurs in patient care units with healthcare professionals in their own working environment...
January 21, 2017: BMC Medical Education
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