Read by QxMD icon Read

Platelet rich plasma

Suruchi Garg
CONTEXT: Platelet-rich plasma (PRP) therapy is finding importance in aesthetic medicine. AIM: The objective of this study was to study efficacy of PRP therapy in follicular unit extraction (FUE) hair transplant. MATERIALS AND METHODS: It is a single-blind, prospective randomised study on 40 FUE hair transplant subjects, allocated in two groups (PRP and non-PRP) alternately. PRP was injected intra-operatively immediately after creating slits over the recipient area in PRP group; and normal saline in non-PRP group...
July 2016: Journal of Cutaneous and Aesthetic Surgery
Alberto de Castro Pochini, Eliane Antonioli, Daniella Zanetti Bucci, Luiz Roberto Sardinha, Carlos Vicente Andreoli, Mario Ferretti, Benno Ejnisman, Anna Carla Goldberg, Moisés Cohen
Objective: To evaluate growth factors and cytokines in samples of platelet-rich plasma obtained by three different centrifugation methods. Methods: Peripheral blood of six individuals with no hematological diseases, aged 18 to 68 years, was drawn to obtain platelet-rich plasma, using the open method and commercial columns by Medtronic and Biomet. The products obtained with the different types of centrifugation were submitted to laboratory analysis, including pro-inflammatory cytokines and chemokines by flow cytometry assays, the concentration of fibroblast growth factors-2 (FGF-2) and transforming growth factor-beta1 (TGF-β1)...
July 2016: Einstein
V Gurewich
The interesting article on the antifibrinolytic effect of Factor XIII by Rijken et al [1] was predicated on the assumption that fibrinolysis is mediated only by tPA, since that is what was studied. However, there is evidence that this common assumption should be put into question. In a study published almost thirty years ago, we showed that whereas spontaneous lysis of platelet poor plasma clots was mediated by tPA, that of platelet-rich plasma clots, with which this article was concerned, could not be abolished by tPA antibody, but instead was abolished by the addition of uPA antibody...
October 17, 2016: Journal of Thrombosis and Haemostasis: JTH
Hideo Masuki, Toshimitsu Okudera, Taisuke Watanebe, Masashi Suzuki, Kazuhiko Nishiyama, Hajime Okudera, Koh Nakata, Kohya Uematsu, Chen-Yao Su, Tomoyuki Kawase
BACKGROUND: The development of platelet-rich fibrin (PRF) drastically simplified the preparation procedure of platelet-concentrated biomaterials, such as platelet-rich plasma (PRP), and facilitated their clinical application. PRF's clinical effectiveness has often been demonstrated in pre-clinical and clinical studies; however, it is still controversial whether growth factors are significantly concentrated in PRF preparations to facilitate wound healing and tissue regeneration. To address this matter, we performed a comparative study of growth factor contents in PRP and its derivatives, such as advanced PRF (A-PRF) and concentrated growth factors (CGF)...
December 2016: Int J Implant Dent
Mito Kobayashi, Tomoyuki Kawase, Kazuhiro Okuda, Larry F Wolff, Hiromasa Yoshie
BACKGROUND: Platelet-rich fibrin (PRF), a platelet-rich plasma (PRP) derivative mainly composed of fibrin networks, has been increasingly demonstrated to be effective in wound healing in clinical and pre-clinical animal studies. However, there has still been a concern that major growth factors may significantly be loss from PRF during its preparation through the slow clotting process. To address this concern, we compared the angiogenic potential of PRF and PRP by standardization of procedures based on volume ratios...
December 2015: Int J Implant Dent
Roman Štukelj, Karin Schara, Apolonija Bedina-Zavec, Vid Šuštar, Manca Pajnič, Ljubiša Pađen, Judita Lea Krek, Veronika Kralj-Iglič, Anita Mrvar-Brečko, Rado Janša
During harvesting of nanovesicles (NVs) from blood, blood cells and other particles in blood are exposed to mechanical forces which may cause activation of platelets, changes of membrane properties, cell deformation and shedding of membrane fragments. We report on the effect of shear forces imposed upon blood samples during the harvesting process, on the concentration of membrane nanovesicles in isolates from blood. Mathematical models of blood flow through the needle during sampling with vacutubes and with free flow were constructed, starting from the Navier-Stokes formalism...
October 10, 2016: European Journal of Pharmaceutical Sciences
Carlos Acosta-Olivo, Jorge Elizondo-Rodriguez, Ricardo Lopez-Cavazos, Felix Vilchez-Cavazos, Mario Simental-Mendia, Oscar Mendoza-Lemus
BACKGROUND: Many treatment options for plantar fasciitis currently exist, some with great success in pain relief. The objective of our study was to compare the use of intralesional steroids with platelet-rich plasma (PRP), using pain scales and functional evaluation, in patients with plantar fasciitis who did not respond to conservative treatment. METHODS: A controlled, randomized, blinded clinical assay was performed. Patients were assigned to one of the two groups by selecting a sealed envelope...
October 11, 2016: Journal of the American Podiatric Medical Association
Gary W Moore, James C Maloney, Robert A Archer, Kerri L Brown, Katarzyna Mayger, Elaine S Bromidge, Mitra F Najafi
BACKGROUND: Autologous platelet-rich plasma (PRP) is gaining increasing use as a wound healing promoter in a variety of clinical settings, including dentistry. Fresh PRP is often used, necessitating daily draws. The present study investigates the possibility of using stored PRP without having to freeze it by storing PRP under variable conditions and assessing growth factor release as a surrogate marker of continued viability. METHODS: Freshly drawn PRP was stored in oxygen permeable and non-oxygen permeable containers under conditions of constant agitation with or without added prostaglandin, intermittent agitation and no agitation, over an 8-day period...
October 8, 2016: British Journal of Biomedical Science
Peter Rubak, Steen D Kristensen, Anne-Mette Hvas
Immature platelets may contain more platelet enzymes such as cyclooxygenase (COX)-1 and COX-2 than mature platelets. Patients with immune thrombocytopenia (ITP) have a higher fraction of immature platelets and can therefore be utilized as a biological model for investigating COX-1 and COX-2 platelet expression. The aims were to develop flow cytometric assays for platelet COX-1 and COX-2 and to investigate the COX-1 and COX-2 platelet expression, platelet turnover, and platelet glycoproteins in ITP patients (n = 10) compared with healthy individuals (n = 30)...
October 7, 2016: Platelets
Mubin Hosnuter, Cem Aslan, Daghan Isik, Gorkem Caliskan, Banu Arslan, Mustafa Durgun
BACKGROUND: Platelet-rich plasma (PRP) is increasingly being used in the treatment of chronic wounds, pathologies of the musculoskeletal system, and in cosmetic medicine; however, the preparation of platelet-rich plasma is both time-consuming and requires invasive intervention. Additional costs are introduced if special equipment is used during preparation. The aim of the present study is to test whether autologous platelet-rich plasma (PRP) preserves the feature of growth factor release when stored at -20 °C after preparation...
October 7, 2016: Journal of Plastic Surgery and Hand Surgery
Florian C Roessler, Sabrina Schumacher, Andreas Sprenger, Ulrich Gärtner, Mohamed Al-Khaled, Jürgen Eggers
BACKGROUND: Patients with acute ischemic strokes frequently take an acetylsalicylic acid (ASA) premedication. We determined the impact of ASA on different thrombolysis strategies in vitro. METHODS: For two clot types made from platelet-rich plasma (one with and one without ASA) lysis rates were measured by weight loss after 1 h for five different groups: in control group A clots were solely placed in plasma; in groups B and C clots were treated with rt-PA (60 kU/ml), and in groups D and E clots were treated with desmoteplase (DSPA; 2 µg/ml)...
October 7, 2016: Journal of Vascular Research
Joshua A Greenspoon, Samuel G Moulton, Peter J Millett, Maximilian Petri
BACKGROUND: Surgical treatment of rotator cuff tears has consistently demonstrated good clinical and functional outcomes. However, in some cases, the rotator cuff fails to heal. While improvements in rotator cuff constructs and biomechanics have been made, the role of biologics to aid healing is currently being investigated. METHODS: A selective literature search was performed and personal surgical experiences are reported. RESULTS: Biologic augmentation of rotator cuff repairs can for example be performed wtableith platelet-rich plasma (PRP) and mesenchymal stem cells (MSCs)...
2016: Open Orthopaedics Journal
L C Hermeto, R DeRossi, R J Oliveira, J R Pesarini, A C M B Antoniolli-Silva, P H A Jardim, A E Santana, E Deffune, J C Rinaldi, L A Justulin
The current study aims to evaluate the macroscopic and histological effects of autologous mesenchymal stem cells (MSC) and platelet-rich plasma on knee articular cartilage regeneration in an experimental model of osteoarthritis. Twenty-four rabbits were randomly divided into four groups: control group, platelet-rich plasma group, autologous MSC undifferentiated group, and autologous MSC differentiated into chondrocyte group. Collagenase solution was used to induce osteoarthritis, and treatments were applied to each group at 6 weeks following osteoarthritis induction...
September 2, 2016: Genetics and Molecular Research: GMR
M İrfan Dönmez, Kubilay İnci, Naciye Dilara Zeybek, H Serkan Doğan, Ali Ergen
PURPOSE: To evaluate the early histological effects of the intravesical instillation of platelet-rich plasma (PRP) in rabbit models of interstitial and hemorrhagic cystitis. METHODS: Thirty-six rabbits were classified into 6 groups: saline (S), S+PRP, hydrochloric acid (HCl), HCl+PRP, cyclophosphamide (CyP), and CyP+PRP. At 48 hours after induction, PRP was prepared and intravesically administered to the S+PRP, HCl+PRP, and CyP+PRP groups. Bladder sections were stained with toluidine blue for mast cell counting and with hematoxylin and eosin for histopathology and mitotic index determination...
September 2016: International Neurourology Journal
Anna Osiecka-Iwan, Stanislaw Moskalewski, Anna Hyc
INTRODUCTION: We have previously observed that rat synovial membranes incubated in medium containing cartilage interstitial fluid (CIF) responded by changes in the expression of hyaluronan synthases (HAS1 and HAS2), collagen type I, versican, aggrecan, lubricin, matrix metalloproteinases 2 and 3 (MMP2 and MMP3), tissue inhibitors of metalloproteinases (TIMP1, 2 and 3), transforming growth factor β1 (TGFβ1), tumor necrosis factor (TNF), interleukin (IL) 1β and IL-6. The aim of the study was to evaluate the influence of particular cytokines found in CIF on the gene expression of extracellular matrix (ECM) proteins in synovial membrane cells...
2016: Folia Histochemica et Cytobiologica
Alice Giacomazzi, Maurizio Degan, Stefano Calabria, Alessandra Meneguzzi, Pietro Minuz
Platelet microparticles (PMPs) contribute to thrombogenesis but the effects of antiplatelet drugs on PMPs generation is undefined. The present study investigated the cellular events regulating PMPs shedding, testing in vitro platelet agonists and inhibitors. Platelet-rich plasma from healthy subjects was stimulated with arachidonic acid (AA), U46619, collagen type-I (10 and 1.5 μg/mL), epinephrine, ADP or TRAP-6 and pre-incubated with acetylsalicylic acid (ASA, 100 and 10 μmol/L), SQ-29,548, apyrase, PSB-0739, or eptifibatide...
2016: Frontiers in Pharmacology
Nan Jiang, Pinggong Du, Weidong Qu, Lin Li, Zhonghao Liu, Songsong Zhu
For several decades, titanium and its alloys have been commonly utilized for endosseous implantable materials, because of their good mechanical properties, chemical resistance, and biocompatibility. But associated low bone mass, wear and loss characteristics, and high coefficients of friction have limited their long-term stable performance, especially in certain abnormal bone-metabolism conditions, such as postmenopausal osteoporosis. In this study, we investigated the effects of platelet-rich plasma (PRP) treatment and TiO2 nanoporous modification on the stability of titanium implants in osteoporotic bone...
2016: International Journal of Nanomedicine
S Witte, C Dedman, F Harriss, G Kelly, Y-M Chang, T H Witte
Superficial digital flexor (SDF) tendonitis is a common injury in Thoroughbred racehorses. Injuries require prolonged rehabilitation, with unpredictable outcomes and a high incidence of re-injury. This observational case-control study aimed to compare race outcomes after commonly advocated treatments for tendon healing. Clinical and racing records were evaluated for 127 National Hunt racehorses treated between 2007 and 2011 for an SDF tendon injury. Two age- and sex-matched control horses were selected for each case horse to analyse the effect on post-injury racing outcomes of pre-injury data, lesion severity and treatment group [controlled exercise alone, bar firing, intralesional platelet-rich plasma (PRP), tendon splitting, tendon splitting combined with bar firing]...
October 2016: Veterinary Journal
Fumiaki Takase, Atsuyuki Inui, Yutaka Mifune, Ryosuke Sakata, Tomoyuki Muto, Yoshifumi Harada, Yasuhiro Ueda, Takeshi Kokubu, Masahiro Kurosaka
Atrophy with fatty degeneration is often seen in rotator cuff muscles with torn tendons. PRP has been reported to enhance tissue repair processes after tendon ruptures. However, the effect of PRP on atrophy and fatty degeneration of the muscle is not yet known. The aim of this study is to examine the effect of PRP on degeneration change of rotator cuff muscles in vitro and in vivo. A murine myogenic cell line and a rat rotator cuff tear model were used in this study and PRP was administrated into subacromial space which is widely used in clinical practice...
September 29, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Geneviève Marcoux, Anne-Claire Duchez, Matthieu Rousseau, Tania Lévesque, Luc H Boudreau, Louis Thibault, Eric Boilard
On activation, platelets release vesicles called microparticles (MPs). MPs are heterogeneous with regard to the presence or absence of mitochondria. We quantified MPs in platelet concentrates (PCs) taking their mitochondrial content into account. Platelet-rich plasma (PRP), buffy coat (BC) and apheresis (AP) PCs were tested through 7 days of storage. A combination of flow cytometry and spanning-tree progression analysis of density-normalized events (SPADE) was used to determine MP and mitochondrial release during storage...
September 29, 2016: Platelets
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"