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Platelet rich plasma

Ilaria Giusti, Marianna Di Francesco, Sandra D'Ascenzo, Paola Palumbo, Anna Rughetti, Luigi Dell'Orso, Paola Annunziata Varasano, Gabriella Lucidi Pressanti, Vincenza Dolo
The clinical use of platelet-rich plasma (PRP) containing or deprived of leukocytes remains a subject of debate and a controversial issue. It is not yet clear whether leukocyte content has a positive or negative effect on tissue healing processes. Several studies, conducted mainly in the orthopedic field, support the use of leukocyte-poor (LP) PRP, whereas other studies have not identified any significant differences between the use of LP and leukocyte-rich PRP. In the present study, the role of leukocytes contained in PRP was assessed to verify their in vitro effect on fibroblasts and endothelial cells, which have a leading role in the biological processes associated with wound healing (including angiogenesis and matrix remodeling)...
April 2018: Experimental and Therapeutic Medicine
Caroline J Reddel, Gabrielle J Pennings, Jennifer L Curnow, Vivien M Chen, Leonard Kritharides
Platelets play an important role in diseases such as cardiovascular disease and cancer, especially through their release of extracellular vesicles (EVs) and role in thrombosis. The effects of the anti-inflammatory drug colchicine on platelets are not well understood. We investigated the effect of colchicine on the release of pro-coagulant EVs from platelets under low-level activation. Citrated platelet-rich plasma (PRP) from healthy donors was incubated with 2 mM colchicine or vehicle at 37°C for 30 minutes with gentle rotation...
March 19, 2018: Thrombosis and Haemostasis
Ulrike Binsker, Raghavendra Palankar, Jan Wesche, Thomas P Kohler, Josephine Prucha, Gerhard Burchhardt, Manfred Rohde, Frank Schmidt, Barbara M Bröker, Uwe Mamat, Jan Pané-Farré, Anica Graf, Patrick Ebner, Andreas Greinacher, Sven Hammerschmidt
Staphylococcus aureus can cause bloodstream infections associated with infective endocarditis (IE) and disseminated intravascular coagulopathy (DIC). Both complications involve platelets. In view of an increasing number of antibiotic-resistant strains, new approaches to control systemic S. aureus infection are gaining importance. Using a repertoire of 52 recombinant S. aureus proteins in flow cytometry-based platelet activation and aggregation assays, we identified, in addition to the extracellular adherence protein Eap, three secreted staphylococcal proteins as novel platelet activating proteins...
March 19, 2018: Thrombosis and Haemostasis
Hieronymus P Stevens, Simone Donners, Julia de Bruijn
Background: Androgenetic alopecia (AGA) is characterized by miniaturization of the hair follicles gradually causing conversion of terminal hairs into vellus hairs, leading to progressive reduction of the density of hair on the scalp. Approved therapeutic options are limited and show side effects. Objectives: To evaluate injections of stromal vascular fraction (SVF), which is rich in adipose-derived stromal cells (ASCs) in combination with platelet-rich plasma (PRP) in the upper scalp as a new autologous treatment option for AGA...
March 15, 2018: Aesthetic Surgery Journal
José M Vilar, Maria E Manera, Angelo Santana, Giuseppe Spinella, Oliver Rodriguez, Mónica Rubio, José M Carrillo, Joaquín Sopena, Miguel Batista
The goal of this study was to objectively assess the effect of a platelet-rich plasma (PRP) derivate in English bulldogs with stifle degenerative joint disease secondary to cranial cruciate ligament rupture (CCLR). We used a force platform and affixed electrogoniometers to measure peak vertical force (PVF), vertical impulse (VI), stance time (ST), and angular range of motion (AROM), from 12 lame client-owned English bulldogs with post-CCLR stifle joint abnormalities. The 12 affected subjects were treated with 4 intra-articular injections of PRP, at 30-day intervals...
2018: PloS One
Paolo Giuseppe Limoli, Enzo Maria Vingolo, Celeste Limoli, Sergio Zaccaria Scalinci, Marcella Nebbioso
This study is aimed at examining whether a suprachoroidal graft of autologous cells can improve best corrected visual acuity (BCVA) and responses to microperimetry (MY) in eyes affected by dry Age-related Macular Degeneration (AMD) over time through the production and secretion of growth factors (GFs) on surrounding tissue. Patients were randomly assigned to each study group. All patients were diagnosed with dry AMD and with BCVA equal to or greater than 1 logarithm of the minimum angle of resolution (logMAR)...
February 12, 2018: Journal of Visualized Experiments: JoVE
Hua-Feng Zhang, Chen-Guang Wang, Hui Li, Yu-Ting Huang, Zhi-Jun Li
Purpose: Platelet-rich plasma (PRP) and hyaluronic acid (HA) have been increasingly used in recent years to treat knee osteoarthritis (OA). However, whether PRP is superior to HA is controversial. Methods: We conducted an electronic search of PubMed, Embase, ScienceDirect, and Cochrane library. The pooled data were analyzed using RevMan 5.1. Results: Three prospective and ten randomized trials were identified. PRP injections reduced pain more effectively than HA injections in OA of the knee at 6 months (mean difference [MD]=-14...
2018: Drug Design, Development and Therapy
Kai Yu, Paula Andruschak, Han Hung Yeh, Dana Grecov, Jayachandran N Kizhakkedathu
The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis. In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions...
March 8, 2018: Biomaterials
Umut Arslan, Emin Özmert, Sibel Demirel, Firdevs Örnek, Figen Şermet
PURPOSE: One of the main reasons for apoptosis and dormant cell phases in degenerative retinal diseases such as retinitis pigmentosa (RP) is growth factor withdrawal in the cellular microenvironment. Growth factors and neurotrophins can significantly slow down retinal degeneration and cell death in animal models. One possible source of autologous growth factors is platelet-rich plasma. The purpose of this study was to determine if subtenon injections of autologous platelet-rich plasma (aPRP) can have beneficial effects on visual function in RP patients by reactivating dormant photoreceptors...
March 15, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
Richard T Amison, Shirin Jamshidi, Khondaker M Rahman, Clive P Page, Simon C Pitchford
Platelet P2Y1 receptor signaling via RhoGTPases is necessary for platelet-dependent leukocyte recruitment, where no platelet aggregation is observed. We investigated signaling cascades involved in distinct P2Y1 -dependent platelet activities in vitro, using specific inhibitors for phospholipase C (PLC) (U73122, to inhibit the canonical pathway), and RhoGTPases: Rac1 (NSC23766) and RhoA (ROCK inhibitor GSK429286). Human platelet rich plasma (for platelet aggregation) or isolated washed platelets (for chemotaxis assays) was treated with U73122, GSK429286 or NSC23766 prior to stimulation with adenosine diphosphate (ADP) or the P2Y1 specific agonist MRS2365...
March 10, 2018: European Journal of Pharmacology
Thomas Parker Vail
No abstract text is available yet for this article.
February 2018: Clinical Orthopaedics and related Research
Matthew T Houdek, Cody C Wyles, Mark S Collins, Benjamin M Howe, Andre Terzic, Atta Behfar, Rafael J Sierra
BACKGROUND: Randomized trials have shown the benefits of injecting bone marrow-derived mesenchymal stem cells (BmMSCs) after standard hip decompression in patients with osteonecrosis of the femoral head. However, the combination of BmMSCs and platelet-rich plasma (PRP) injected into the femoral head after decompression has not been reported previously. This study reports the results in a preliminary series of patients with osteonecrosis of the femoral head treated with BmMSCs plus PRP...
February 2018: Clinical Orthopaedics and related Research
Mohamed Hussein Medhat El-Komy, Noha Adly Saleh, Marwah Adly Saleh
Oral erosions of pemphigus vulgaris (PV) represent a therapeutic challenge. In this work we compared intralesional injection of steroids (ILS) and autologous platelet-rich plasma (PRP) treatment of oral erosions of PV. In this split-mouth randomized double blind study, one side of the buccal mucosa of 11 PV patients was injected with 10 mg/ml triamcinolone and the opposite side was injected with 1 millilitre of autologous PRP every 14 days for 3 months. The percentage of clinical improvement, pemphigus disease area index and pain score were recorded before and after therapy...
March 10, 2018: Archives of Dermatological Research
Mohammad-Hossein Beigi, Atefeh Atefi, Hamid-Reza Ghanaei, Sheyda Labbaf, Fatemeh Ejeian, Mohammad-Hossein Nasr-Esfahani
In the current study the effect of superimposing platelet-rich plasma (PRP) on different culture mediums in a three-dimensional (3D) alginate scaffold encapsulated with adipose mesenchymal stem cells (ADSCs) for cartilage tissue repair is reported. The 3D alginate scaffolds with co-administration of PRP and/or chondrogenic supplements had a significant effect on the differentiation of ADSCs into mature cartilage, as assessed by evaluation of the expression of cartilage related markers of Sox9, Collagen II, Aggrecan and collagen and glycosaminoglycan assays...
March 9, 2018: Journal of Tissue Engineering and Regenerative Medicine
Jenny F López, Jertta-Riina Sarkanen, Outi Huttala, Ilkka S Kaartinen, Hannu O Kuokkanen, Timo Ylikomi
Growth factors are the key elements in wound healing signaling for cell migration, differentiation and proliferation. Platelet-rich plasma (PRP), one of the most studied sources of growth factors, has demonstrated to promote wound healing in vitro and in vivo. Adipose tissue is an alternative source of growth factors. Through a simple lipoaspirate method, adipose derived growth factor-rich preparation (adipose tissue extract; ATE) can be obtained. The authors set out to compare the effects of these two growth factor sources in cell proliferation and migration (scratch) assays of keratinocyte, fibroblast, endothelial and adipose derived stem cells...
March 6, 2018: Cytotechnology
Ricardo Bastos, Marcelo Mathias, Renato Andrade, Raquel Bastos, Alex Balduino, Vinicius Schott, Scott Rodeo, João Espregueira-Mendes
PURPOSE: To compare the effectiveness and safety of intra-articular injections of autologous expanded mesenchymal stromal stem cells alone (MSCs), or in combination with platelet-rich plasma (MSCs + PRP), in patients with knee osteoarthritis. METHODS: Eighteen patients (57.6 ± 9.6 years) with radiographic symptomatic knee osteoarthritis (Dejour grades II-IV) were randomized to receive intra-articular injections of MSCs (n = 9) or MSCs + PRP (n = 9)...
March 6, 2018: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
Eun Young Ko, Shivraj Hariram Nile, Yi-Sook Jung, Young Soo Keum
In this study, we reported the potential of various methanol fractions of onion (MFO) and its components quercetin and quercetin glucosides on platelet aggregation and antioxidant activity. The onion extracts were separated into several fractions using methanol and water. Further, these extracts were analyzed using simple high-performance liquid chromatography-diode array detector method to separate quercetin (Q), quercetin-4'- O -monoglucoside and quercetin-3, 4'- O -diglucoside from onion sample. It was observed that the bioactive compounds were accumulated in the non-polar portions rather than in the polar one...
March 2018: 3 Biotech
David M Hoganson, Dane A Cooper, Kimberly N Rich, Breanna L Piekarski, Liqiong Gui, Joseph P Gaut, John E Mayer, Elena Aikawa, Laura E Niklason, Sitaram M Emani
BACKGROUND: Synthetic graft materials are commonly used for shunts and cardiovascular reconstruction in neonates but are prone to thrombosis and scarring. Umbilical vein is a potential source of autologous, endothelialized tissue for neonatal shunts and tissue reconstruction but requires preservation prior to implantation. METHODS: Umbilical cords were collected in UW solution with antibiotics at 4 °C until dissection. Umbilical vein segments were tested for burst pressure before and after two weeks of preservation...
March 3, 2018: Annals of Thoracic Surgery
S Guner, S I Guner, M A Gokalp, M F Ceylan, S S Unsal, H Demir
OBJECTIVE: In view of the high occurrence of avascular necrosis of the femoral head (ANFH) after femoral neck fracture and the difficulties in the treatment, our work aimed to explore the effects of platelet-rich plasma (PRP) combined with tri-calcium phosphate (TCP) on the repair of ANFH after femoral neck fracture and to provide reference for clinical treatment. MATERIALS AND METHODS: Thirty New Zealand white rabbits were randomly divided into control group, TCP group, and PRP+TCP group...
February 2018: European Review for Medical and Pharmacological Sciences
X-L Zhang, Y-M Wang, K Chu, Z-H Wang, Y-H Liu, L-H Jiang, X Chen, Z-Y Zhou, G Yin
OBJECTIVE: In view of the high occurrence of avascular necrosis of the femoral head (ANFH) after femoral neck fracture and the difficulties in the treatment, our work aimed to explore the effects of platelet-rich plasma (PRP) combined with tri-calcium phosphate (TCP) on the repair of ANFH after femoral neck fracture and to provide reference for clinical treatment. MATERIALS AND METHODS: Thirty New Zealand white rabbits were randomly divided into control group, TCP group, and PRP+TCP group...
February 2018: European Review for Medical and Pharmacological Sciences
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