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https://www.readbyqxmd.com/read/28820472/mussel-inspired-dopamine-and-carbon-nanotube-leading-to-a-biocompatible-self-rolling-conductive-hydrogel-film
#1
Junzi Jiang, Yong Huang, Yitian Wang, Hui Xu, Malcolm Xing, Wen Zhong
We report a novel self-rolling, conductive, and biocompatible multiwall carbon nanotube (MWCNT)-dopamine-polyethylene glycol (PEG) hydrogel film. The gel can self-fold into a thin tube when it is transferred from a glass slide to an aqueous environment, regardless of the concentrations of the MWCNT. The film presents a highly organized pattern, which results from the self-assembly of hydrophilic dopamine and hydrophobic carbon nanotubes. By exploring the biomedical potential, we found that MWCNT-included rolled film is nontoxic and can promote cell growth...
August 18, 2017: Materials
https://www.readbyqxmd.com/read/28816358/trpc3-mediated-ca-2-signals-as-a-promising-strategy-to-boost-therapeutic-angiogenesis-in-failing-hearts-the-role-of-autologous-endothelial-colony-forming-cells
#2
REVIEW
Francesco Moccia, Angela Lucariello, Germano Guerra
Endothelial progenitor cells (EPCs) are a sub-population of bone marrow-derived mononuclear cells that are released in circulation to restore damaged endothelium during its physiological turnover or rescue blood perfusion after an ischemic insult. Additionally, they may be mobilized from perivascular niches located within larger arteries' wall in response to hypoxic conditions. For this reason, EPCs have been regarded as an effective tool to promote revascularization and functional recovery of ischemic hearts, but clinical application failed to exploit the full potential of patients-derived cells...
August 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28816271/-construction-and-improvement-of-animal-models-with-different-positional-osseous-metastasis-of-prostate-cancer-in-vivo
#3
Y X Bi, M H Xiao, N N Zhang, X Y Li, X P Mao, K Zhang, Z R Zhang, L Y Zhao
OBJECTIVE: To provide an important tool for the study of diagnose and treatment of prostate cancer (PCa) osseous metastasis and change of bone stress force on prostate cancer (PCa) osseous metastasis and a platform, which is more congruous to clinical process, for prevention and cure of neoplastic bone metastases, and to carry out the construction and improvement of animal models of PCa with different positional osseous metastasis in vivo. METHODS: Different gradient concentrations of RM-1 cells were inoculated into the cavity of left femoral bone or lumbar vertebra of mice (C57BL/6) respectively...
August 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28814530/a-high-fat-diet-decreases-bone-mass-in-growing-mice-with-systemic-chronic-inflammation-induced-by-low-dose-slow-release-lipopolysaccharide-pellets
#4
Jay J Cao, Brian R Gregoire, Chwan-Li Shen
Background: Chronic inflammation is associated with increased bone resorption and is linked to osteopenia, or low bone mass. Obesity is also associated with low-grade chronic upregulation of inflammatory cytokines.Objective: This study investigated the effect of high-fat (HF) diet-induced obesity on bone structure changes in growing mice with existing systemic chronic inflammation induced by low-dose, slow-release lipopolysaccharide (LPS).Methods: Forty-eight 6-wk-old female C57BL/6 mice were randomly assigned to 4 treatment groups (n = 12/group) in a 2 × 2 factorial design-control (placebo) or LPS treatment (1...
August 16, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28811932/two-novel-direct-spio-labels-and-in%C3%A2-vivo-mri-detection-of-labeled-cells-after-acute-myocardial-infarct
#5
Riikka M Korpi, Kirsi Alestalo, Timo Ruuska, Eveliina Lammentausta, Ronald Borra, Fredrik Yannopoulos, Siri Lehtonen, Jarkko T Korpi, Elisa Lappi-Blanco, Vesa Anttila, Petri Lehenkari, Tatu Juvonen, Roberto Blanco Sequieros
BACKGROUND: Acute myocardial infarction (AMI) is a leading cause of morbidity and mortality worldwide. Cellular decay due hypoxia requires rapid and validated methods for possible therapeutic cell transplantation. PURPOSE: To develop direct and rapid superparamagnetic iron oxide (SPIO) cell label for a large-animal model and to assess in vivo cell targeting by magnetic resonance imaging (MRI) in an experimental AMI model. MATERIAL AND METHODS: Bone marrow mononuclear cells (BMMNCs) were labeled with SPIO particles using two novel direct labeling methods (rotating incubation method and electroporation)...
August 2017: Acta Radiologica Open
https://www.readbyqxmd.com/read/28806276/circulating-progenitor-cell-response-to-exercise-in-wheelchair-racing-athletes
#6
Grace M Niemiro, Thomas Edwards, J P Barfield, Joseph W Beals, Elizabeth M Broad, Robert W Motl, Nicholas A Burd, Lara A Pilutti, Michael De Lisio
INTRODUCTION: Circulating progenitor cells (CPCs) are a heterogeneous population of stem/progenitor cells in peripheral blood that participate in tissue repair. CPC mobilization has been well characterized in able-bodied persons, but has not been previously investigated in wheelchair racing athletes. The purpose of this study was to characterize CPC and CPC sub-population mobilization in elite wheelchair racing athletes in response to acute, upper-extremity aerobic exercise to determine if CPC responses are similar to ambulatory populations...
August 11, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28804955/3m-052-as-an-adjuvant-for-a-plga-microparticle-based-leishmania-donovani-recombinant-protein-vaccine
#7
Qian Wang, Meagan A Barry, Christopher A Seid, Elissa M Hudspeth, C Patrick McAtee, Michael J Heffernan
It is believed that an effective vaccine against leishmaniasis will require a T helper type 1 (TH 1) immune response. In this study, we investigated the adjuvanticity of the Toll-like receptor (TLR) 7/8 agonist 3M-052 in combination with the Leishmania donovani 36-kDa nucleoside hydrolase recombinant protein antigen (NH36). NH36 and 3M-052 were encapsulated in separate batches of poly(lactic-co-glycolic acid) (PLGA) microparticles (MPs). The loading efficiency for NH36 was 83% and for 3M-052 was above 95%. In vitro stimulation of bone marrow-derived dendritic cells, measured by IL-12 secretion, demonstrated that 3M-052 (free or MP-formulated) had a concentration-dependent immunostimulatory effect with an optimum concentration of 2 µg/mL...
August 14, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28804813/a-systematic-review-of-the-clinical-applications-and-complications-of-bone-marrow-aspirate-concentrate-in-management-of-bone-defects-and-nonunions
#8
REVIEW
Mohamed A Imam, James Holton, Lukas Ernstbrunner, Wojciech Pepke, Florian Grubhofer, Ali Narvani, Martyn Snow
PURPOSE: Fracture healing encompasses a succession of dynamic multifactorial metabolic events, which ultimately re-establishes the integrity of the biomechanical properties of the bone. Up to 10% of the fractures occurring annually will need additional surgical procedures because of impaired healing. The aim of this article is to review the current literature regarding the use of bone marrow aspirate concentrate (BMAC) and its effectiveness in the management of bone defects. METHODS: We have included all published clinical literature investigating the development, techniques and applications of BMAC...
August 13, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28804540/restoration-of-bone-defects-using-modified-heterogeneous-deproteinized-bone-seeded-with-bone-marrow-mesenchymal-stem-cells
#9
Jun Li, Zeyu Huang, Liyan Chen, Xin Tang, Yue Fang, Lei Liu
The aim of the present study was to investigate the effect of modified heterogeneous deproteinized bone combined with bone marrow mesenchymal stem cells (BMSCs) in the restoration of a validated bone defect model. BMSCs were identified by flow cytometry and multilineage differentiation assay. The structural features of the modified heterogeneous deproteinized bone scaffold and biocompatibility between BMSCs and the scaffold were confirmed by scanning electron microscope (SEM) detection. The cytotoxicity of the modified heterogeneous deproteinized bone scaffolds were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide (MTT) assay...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28802849/silk-fibroin-chitosan-scaffold-with-tunable-properties-and-low-inflammatory-response-assists-the-differentiation-of-bone-marrow-mesenchymal-stem-cells
#10
Da-Wei Li, Xiaohua Lei, Feng-Li He, Jin He, Ya-Li Liu, Ya-Jing Ye, Xudong Deng, Enkui Duan, Da-Chuan Yin
The physical and chemical properties of the scaffold are known to play important roles in three-dimensional (3D) cell culture, which always determine the cellular fate or the results of implantation. To control these properties becomes necessary for meeting the requirements of a variety of tissue engineering applications. In this study, a series of silk fibroin/chitosan (SF/CS) scaffolds with tunable properties were prepared using freeze-drying method, and the rat bone marrow-derived mesenchymal stem cells (BM-MSCs) were seeded in these scaffolds to evaluate their availability of use in tissue engineering...
August 9, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28800701/a-new-indole-derivative-decreased-sall4-gene-expression-in-acute-promyelocytic-leukemia-cell-line-nb4
#11
Zahra Sheikhrezaei, Parisa Heydari, Alireza Farsinezhad, Ahmad Fatemi, Soudeh Khanamani Falahati-Pour, Shokoofeh Darakhshan, Mojgan Noroozi Karimabad, Ali Darekordi, Hossein Khorramdelazad, Gholamhossein Hassanshahi
Background: Acute myeloblastic leukemia (AML) is a clonal disorder due to bone marrow failure and uncontrolled proliferation of myeloid lineage. Acute promyelocytic leukemia (APL) is a subtype of AML. Heterocyclic compounds, such as indole, are considered as attractive candidates for cancer therapy, due to their abundance in nature and known biological activity. Sal-like protein (SALL4) is a zinc finger transcription factor involving in the multi-potency of stem cells, in the NB4 cell line...
August 12, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28799674/ultrastructural-characteristics-of-ovine-bone-marrow-derived-mesenchymal-stromal-cells-cultured-with-a-silicon-stabilized-tricalcium-phosphate-bioceramic
#12
Salvatore Desantis, Gianluca Accogli, Janina Burk, Sara Zizza, Maria Mastrodonato, Edda G Francioso, Roberta Rossi, Antonio Crovace, Leonardo Resta
Bioceramics are being used in experimental bone engineering application in association with bone marrow derived mesenchymal stem cells (BM-MSCs) as a new therapeutic tool, but their effects on the ultrastructure of BM-MSCs are yet unknown. In this study we report the morphological features of ovine (o)BM-MSCs cultured with Skelite, a resorbable bioceramic based on silicon stabilized tricalcium phosphate (SiTCP), able to promote the repair of induced bone defect in sheep model. oBM-MSCs were isolated from the iliac crest, cultured until they reached near-confluence and incubated with SiTCP...
August 11, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28798905/enrofloxacin-and-toltrazuril-are-able-to-reduce-toxoplasma-gondii-growth-in-human-bewo-trophoblastic-cells-and-villous-explants-from-human-third-trimester-pregnancy
#13
Rafaela J da Silva, Angelica O Gomes, Priscila S Franco, Ariane S Pereira, Iliana C B Milian, Mayara Ribeiro, Paolo Fiorenzani, Maria C Dos Santos, José R Mineo, Neide M da Silva, Eloisa A V Ferro, Bellisa de Freitas Barbosa
Classical treatment for congenital toxoplasmosis is based on combination of sulfadiazine and pyrimethamine plus folinic acid. Due to teratogenic effects and bone marrow suppression caused by pyrimethamine, the establishment of new therapeutic strategies is indispensable to minimize the side effects and improve the control of infection. Previous studies demonstrated that enrofloxacin and toltrazuril reduced the incidence of Neospora caninum and Toxoplasma gondii infection. The aim of the present study was to evaluate the efficacy of enrofloxacin and toltrazuril in the control of T...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28798722/autonomic-neuropathy-and-albuminocytologic-dissociation-in-cerebrospinal-fluid-as-the-presenting-features-of-primary-amyloidosis-a-case-report
#14
Jingjing Li, Yi Li, Hongbing Chen, Shihui Xing, Huiyu Feng, Dawei Liu, Dilong Wang, Jinsheng Zeng, Yuhua Fan
OBJECTIVE: Primary amyloidosis is a disease with a poor prognosis and multi-organ involvement. Here, we report the clinical and pathological features of a patient with primary amyloidosis featuring autonomic neuropathy as the initial symptom and albuminocytologic dissociation in the cerebrospinal fluid (CSF). METHODS: The patient was a 60-year-old Chinese male with numbness, orthostatic hypotension, and gastrointestinal symptoms. For diagnosis, we performed an electromyogram (EMG), lumbar puncture, Bence Jones protein urine test, serum electrophoresis blood test, sural nerve and rectal membrane biopsies, transthyretin (TTR) gene sequencing, and bone marrow puncture...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28792693/serum-hepcidin-levels-in-multiple-myeloma
#15
Manolov Victor, Hadjiev Evgeniy, Tzvetkova Gergana, Petrova Julia, Vasilev Vasil, Marinov Borislav, Bogov Ivo, Gramatikova Zlatina, Tzatchev Kamen
BACKGROUND: Multiple myeloma (MM) is a malignant disease with a 10% frequency among all haematology neoplasms. It is characterized by clone proliferation of plasmatic cells in bone marrow, monoclonal gammopathy, and anemia, hypercalcemia, and kidney failure and bone lesions. IL-6 is an inflammatory cytokine, potential growth factor for myeloma cells, as elevated serum levels are connected with poor disease prognosis. IL-6 modulates many gene transcriptions, encoding synthesis of acute phase proteins, including C-reactive protein (CRP) and hepcidin...
July 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28791738/metformin-promotes-neuronal-differentiation-and-neurite-outgrowth-through-ampk-activation-in-human-bone-marrow-mesenchymal-stem-cells
#16
Min-Ji Ahn, Goang-Won Cho
Metformin is an AMP-activated kinase (AMPK) activator that plays a role in glucose energy metabolism and cell protection. It is widely used to treat several diseases, including type 2 diabetes, cardiovascular diseases, cancer, and metabolic diseases. In this study, we investigated whether AMPK activation upon treatment with metformin may promote neurite outgrowth during the progression of neuronal differentiation in human bone marrow-mesenchymal stem cells (hBM-MSCs). Differentiation of metformin-treated MSCs (Met-MSCs to Met-diMSCs) in the neuronal induction media resulted in an increase in the number of differentiated cells in a metformin concentration-dependent manner...
August 9, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28790502/actin-up-in-the-nucleus-regulation-of-actin-structures-modulates-mesenchymal-stem-cell-differentiation
#17
Janet Rubin, Buer Sen
Stem cells respond to environmental signals that induce their differentiation to cells that make up specialized tissues and organs. Our laboratory has focused on bone marrow mesenchymal stem cells (MSCs) that supply bone osteoblasts and marrow adipocytes, an output that appears to be reciprocal. Case in point: exercise promotes osteogenesis and bone formation, and inhibits marrow adipose accrual. A mechanically induced signal pathway concentrating on preserving β-catenin also causes increased structure of the actin cytoskeleton, both of which inhibit adipogenesis...
2017: Transactions of the American Clinical and Climatological Association
https://www.readbyqxmd.com/read/28782873/stable-morphological-physiological-and-neural-protein-expression-changes-in-rat-bone-marrow-mesenchymal-stem-cells-treated-with-electromagnetic-field-and-nitric-oxide
#18
Nazanin Haghighat, Parviz Abdolmaleki, Mehrdad Behmanesh, Mohammad Satari
As an external physical factor, electromagnetic field (EMF) may influence cellular processes and nitric oxide (NO) by serving as a secondary messenger molecule in intracellular signaling cascades. Effects of these factors were evaluated simultaneously on viability, morphology, and variation of calcium ion content, and neural protein marker expression in rat bone marrow mesenchymal stem cells (BMSCs). The mesenchymal stem cells were isolated from rat bone marrow and cultured. Deta-NO as a donor molecule of NO was added to cell culture medium after several passages...
August 7, 2017: Bioelectromagnetics
https://www.readbyqxmd.com/read/28780774/changes-in-haematopoietic-progenitor-colony-differentiation-and-proliferation-and-the-production-of-different-abzymes-in-eae-mice-treated-with-dna
#19
Kseniya S Aulova, Ludmila B Toporkova, Julia A Lopatnikova, Alina A Alshevskaya, Sergei V Sennikov, Valentina N Buneva, Thomas Budde, Sven G Meuth, Nelly A Popova, Irina A Orlovskaya, Georgy A Nevinsky
Immunization of experimental autoimmune encephalomyelitis (EAE)-prone C57BL/6 mice with MOG35-55 (a model used to study aspects of human multiple sclerosis) is known to lead to the production of various abzymes. The production of catalytic IgGs that can efficiently hydrolyse myelin basic protein (MBP), MOG and DNA is associated with changes in the profile of differentiation and level of proliferation of mice bone marrow haematopoietic stem cells (HSCs). As MOG simulates the production of abzymes with high DNase activity, we compared the effects of DNA and MOG immunization on EAE-prone mice...
August 5, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28771866/cellular-behaviours-of-bone-marrow-derived-mesenchymal-stem-cells-towards-pristine-graphene-oxide-nanosheets
#20
Changbo Wei, Zifeng Liu, Fangfang Jiang, Binghui Zeng, Mingdi Huang, Dongsheng Yu
OBJECTIVES: Graphene oxide (GO), the derivative of graphene with unique properties, has attracted much attention for applications in dental implants. The aim of this study was, by two biomimetic cell culture methods, to investigate the quantitative relationship between the concentration of pristine GO nanosheets and their cellular behaviours towards bone marrow-derived mesenchymal stem cells (BMSCs). MATERIALS AND METHODS: The cells were firstly characterized according to their morphology, self-renewal capabilities and multipotency...
August 3, 2017: Cell Proliferation
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