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https://www.readbyqxmd.com/read/28551207/genotoxic-and-mutagenic-assessment-of-iron-oxide-maghemite-%C3%AE-fe2o3-nanoparticle-in-the-guppy-poecilia-reticulata
#1
Gabriel Qualhato, Thiago Lopes Rocha, Emília Celma de Oliveira Lima, Daniela Melo E Silva, Júlio Roquete Cardoso, Cesar Koppe Grisolia, Simone Maria Teixeira de Sabóia-Morais
The environmental risk of nanomaterials (NMs) designed and used in nanoremediation process is of emerging concern, but their ecotoxic effects to aquatic organism remains unclear. In this study, the citrate-coated (maghemite) nanoparticles (IONPs) were synthesized and its genotoxic and mutagenic effects were investigated in the female guppy Poecilia reticulata. Fish were exposed to IONPs at environmentally relevant iron concentration (0.3 mg L(-1)) during 21 days and the animals were collected at the beginning of the experiment and after 3, 7, 14 and 21 days of exposure...
May 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28550622/genomic-instability-related-to-zinc-deficiency-and-excess-in-an-in-vitro-model-is-the-upper-estimate-of-the-physiological-requirements-recommended-for-children-safe
#2
Gisel Padula, María Virginia Ponzinibbio, Rocío Celeste Gambaro, Analía Isabel Seoane
Micronutrients are important for the prevention of degenerative diseases due to their role in maintaining genomic stability. Therefore, there is international concern about the need to redefine the optimal mineral and vitamin requirements to prevent DNA damage. We analyzed the cytostatic, cytotoxic, and genotoxic effect of in vitro zinc supplementation to determine the effects of zinc deficiency and excess and whether the upper estimate of the physiological requirement recommended for children is safe. To achieve zinc deficiency, DMEM/Ham's F12 medium (HF12) was chelated (HF12Q)...
May 26, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28544661/early-passage-mesenchymal-stem-cells-display-decreased-radiosensitivity-and-increased-dna-repair-activity
#3
Po-Kuei Wu, Jir-You Wang, Cheng-Fong Chen, Kuang-Yu Chao, Ming-Chau Chang, Wei-Ming Chen, Shih-Chieh Hung
Cell therapies using human mesenchymal stem cells (MSCs) have received much attention in the past decade. In pursuit of the therapeutic potential of MSCs, cell expansion is required to generate a great number of cells with desired phenotype and functionality. Long-term expansion in vitro, however, can lead to altered functions. To explore the changes in DNA damage responses (DDR) in MSCs expanded, DDR pathways following irradiation were characterized in early- and late-passage bone marrow MSCs. Seventy-two hours after irradiation, the percentage of sub-G1 cells in early-passage MSCs did not change significantly...
June 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28540821/enhancing-radiosensitisation-of-brca2-proficient-and-brca2-deficient-cell-lines-with-hyperthermia-and-parp1-i
#4
Arlene L Oei, Vidhula R Ahire, C M van Leeuwen, Rosemarie Ten Cate, Lukas J A Stalpers, Johannes Crezee, H Petra Kok, Nicolaas A P Franken
Poly(ADP-ribose)polymerase1 (PARP1) is an important enzyme in regulating DNA replication. Inhibition of PARP1 can lead to collapsed DNA forks which subsequently causes genomic instability, making DNA more susceptible in developing fatal DNA double strand breaks. PARP1-induced DNA damage is generally repaired by homologous recombination (HR), in which BRCA2 proteins are essential. Therefore, BRCA2-deficient tumour cells are susceptible to treatment with PARP1-inhibitors (PARP1-i). Recently, BRCA2 was shown to be down-regulated by hyperthermia (HT) temporarily, and this consequently inactivated HR for several hours...
May 19, 2017: International Journal of Hyperthermia
https://www.readbyqxmd.com/read/28536798/pollutants-induced-oxidative-stress-dna-damage-and-cellular-deformities-in-clarias-gariepinus-burchell-from-river-yamuna-in-delhi-region-india
#5
Anil K Tyor, Kanika Pahwa
The purpose of the present study was to evaluate the severity of pollution impact at two extreme sites of river Yamuna in Delhi region utilizing oxidative stress, genotoxic and histopathological biomarkers in gonad (ovary) of Clarias gariepinus. To evaluate oxidative stress, TBAR's and ferric reducing antioxidant power (FRAP) assay were employed while comet assay and classic histology were used to estimate genotoxicity and cellular damage respectively. The results indicated significant increase (p < 0...
May 23, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28535953/genotoxic-potential-of-dental-bulk-fill-resin-composites
#6
Tobias T Tauböck, Danijela Marovic, Davor Zeljezic, Andrea D Steingruber, Thomas Attin, Zrinka Tarle
OBJECTIVE: To investigate both genotoxicity and hardening of bulk-fill composite materials applied in 4-mm layer thickness and photo-activated for different exposure times. METHODS: Three flowable bulk-fill materials and one conventional flowable composite were filled in molds (height: 4mm) and irradiated for 20 or 30s. The top (0mm) and bottom (4mm) specimen surface were mechanically scraped, and eluates (0.01g composite in 1.5ml RPMI 1640 cell culture media) prepared for each material, surface level and irradiation time...
May 20, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28535484/biomonitoring-of-genotoxicity-of-industrial-fertilizer-pollutants-in-aiolopus-thalassinus-orthoptera-acrididae-using-alkaline-comet-assay
#7
Eman A Abdelfattah, Maria Augustyniak, Hesham A Yousef
Phosphate fertilizer industry is considered as one of the main sources of environmental pollutants. Besides solid waste products, e.g. phosphates, sulphates, and heavy metals, also atmospheric pollutants, such as hydrofluoric acid fumes (HF), sulphur dioxide (SO2), nitrogen oxides (NO2), and particulate matter with diameter up to 10 μm (PM10) can be dangerous. Genotoxic effect of these pollutants was monitored by assessing the DNA damage using alkaline comet assay on cells from brain, thoracic muscles and gut of Aiolopus thalassinus collected at three sites (A-C) located at 1, 3, and 6 km away from Abu-Zaabal Company for Fertilizers and Chemical Industries...
May 16, 2017: Chemosphere
https://www.readbyqxmd.com/read/28533948/radiosensitization-effect-of-hsa-mir-138-2-3p-on-human-laryngeal-cancer-stem-cells
#8
Ying Zhu, Li-Yun Shi, Yan-Min Lei, Yan-Hong Bao, Zhao-Yang Li, Fei Ding, Gui-Ting Zhu, Qing-Qing Wang, Chang-Xin Huang
BACKGROUND: Treatments that target cancer stem cells play an important role in the controlling and eliminating of tumor initiation as well as in development, progression, and chemotherapy/radiotherapy resistance. In our previous study, we cultured and harvested human laryngeal cancer stem cells (CSCs) and applied microRNA biochips to screen differentially expressed miRNAs that were related to radiation tolerance in irradiated human laryngeal CSCs. According to the predicted genes and pathways of differential miRNAs target, down-regulated expression of hsa-miR-138-2-3p under radiation was thought to play a key role in enhancing the radio-sensitivity in human laryngeal squamous cancer stem cells...
2017: PeerJ
https://www.readbyqxmd.com/read/28533085/return-to-drive-after-non-evolutive-brain-damage-french-recommendations
#9
REVIEW
Anne-Claire D'apolito, Jean-Luc Leguiet, Michel Enjalbert, Francis Lemoine, Jean-Michel Mazaux
Return to drive after brain damage is a crucial question either for patients than health professionals. The Société française de medicine physique et de réadaptation (SOFMER) and Comète France association developed recommandations for patient's identification, evaluation and accompaniment as part of their project to resume to drive. The place of rehabilitation process and patient's focus has been also discussed. AIMS: Using a literature review, the aim was to define clinical pathways to determine people who need a fitness to drive evaluation after a non-evolutive brain damage as well as the assessment process...
May 19, 2017: Annals of Physical and Rehabilitation Medicine
https://www.readbyqxmd.com/read/28532341/pterostilbene-protects-against-uvb-induced-photo-damage-through-a-phosphatidylinositol-3-kinase-dependent-nrf2-are-pathway-in-human-keratinocytes
#10
Huaping Li, Na Jiang, Bihua Liang, Qing Liu, Erting Zhang, Liqian Peng, Huiyan Deng, Runxiang Li, Zhenjie Li, Huilan Zhu
OBJECTIVE: Ultraviolet B (UVB) irradiation is the initial etiological factor for various skin disorders, including erythema, sunburn, photoaging, and photocarcinogenesis. Pterostilbene (Pter) displayed remarkable antioxidant, anti-inflammatory, and anticarcinogenic activities. This study aimed to investigate the effective mechanism of Pter against UVB-induced photodamage in immortalized human keratinocytes. METHODS: Human keratinocytes were pretreated with Pter (5 and 10 μM) for 24 h prior to UVB irradiation (300 mJ/cm(2))...
May 22, 2017: Redox Report: Communications in Free Radical Research
https://www.readbyqxmd.com/read/28531839/genotoxic-and-mutagenic-effects-of-atrazine-atanor-50-sc-on-dendropsophus-minutus-peters-1872-anura-hylidae-developmental-larval-stages
#11
Macks Wendhell Gonçalves, Calebe Bertolino Marins de Campos, Vinícius Guerra Batista, Aparecido Divino da Cruz, Paulo de Marco Junior, Rogério Pereira Bastos, Daniela de Melo E Silva
The potential mutagenic and genotoxic effects of the herbicide atrazine were investigated in different developmental stages of Dendropsophus minutus tadpoles. These animals were exposed to 4 nominal concentrations of atrazine (2.25, 4.5, 9, and 18 mg/L) and 40 mg/L of Cyclophosphamide as a positive control, for 96 h. Negative controls were also added to the experiment. The tadpoles were divided into three groups according to Gosner's developmental stages, namely GS 25-33 as premetamorphic, GS 36-39 as prometamorphic, and GS 42-43 as metamorphic climax...
May 16, 2017: Chemosphere
https://www.readbyqxmd.com/read/28531838/antimicrobial-agents-triclosan-chloroxylenol-methylisothiazolinone-and-borax-used-in-cleaning-had-genotoxic-and-histopathologic-effects-on-rainbow-trout
#12
Erol Capkin, Tuna Ozcelep, Sevki Kayis, Ilhan Altinok
Triclosan (TRC), chloroxylenol (PCMX) and methylisothiazolinone (MIT) have been commonly used as an antimicrobial in soaps while borax (BRX) is used in household cleaning. After using these chemicals, they are washed down drains and getting into the aquatic ecosystem in which they may affect aquatic living organisms. In the present study, the chronic effects of TRC, PCMX, MIT and BRX on genotoxicity, gene expression and histopathology of rainbow trout (Oncorhynchus mykiss) were evaluated for 40 days under semi static condition...
May 15, 2017: Chemosphere
https://www.readbyqxmd.com/read/28528796/zinc-oxide-nanoparticle-energy-band-gap-reduction-triggers-the-oxidative-stress-resulting-into-autophagy-mediated-apoptotic-cell-death
#13
Manoranjan Arakha, Jyoti Roy, Parth Sarthi Nayak, Bibekanand Mallick, Suman Jha
The physico-chemical properties of nanoparticle (NP), such as particle size, surface defects, crystallinity and accessible surface, affect NP photocatalytic activity that in turn defines the NP cytotoxic propensity. Since zinc oxide nanoparticle (ZnONP) energy band gap falls in a range of a semiconductor, the particle possesses photocatalytic activity. Hence, the study correlates energy band gap with cytotoxic propensity of ZnONP. To this end, ZnONPs with varying energy band gap are fabricated by varying calcination temperature...
May 18, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28525815/an-integrated-approach-to-study-the-risk-from-landfill-soil-of-delhi-chemical-analyses-in-vitro-assays-and-human-risk-assessment
#14
Swati, Pooja Ghosh, Indu Shekhar Thakur
In the present study, landfill soil of three municipal solid waste landfill sites of Delhi, India were toxico-chemically analyzed for human risk assessment as inadequate information is available on the possible health effects of the contaminants present in landfill soil. The landfill soil samples were prepared for analyzing heavy metal concentration, organic contaminants and toxicity analysis separately. Composite soil sample collected from three landfill sites were analyzed for heavy metal by ICP-AES. Metal concentration so obtained was below the permissible limit of soil but higher than the set limits for effluent...
May 16, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28525650/detection-of-damage-on-single-or-double-stranded-dna-in-a-population-exposed-to-arsenic-in-drinking-water
#15
J Jiménez-Villarreal, D I Rivas-Armendariz, C P Pineda-Belmontes, N D Betancourt-Martínez, M A Macías-Corral, A J Guerra-Alanis, M S Niño-Castañeda, J Morán-Martínez
Different studies have suggested an association between arsenic (As) exposure and damage to single-stranded DNA by reactive oxygen species derived from the biotransformation of arsenic. The single strand damages are converted to double strand damage upon interaction with ultraviolet radiation. Analysis of genomic integrity is important for assessing the genotoxicity caused by environmental pollutants. In this study, we compared the concentration of As in drinking water, nutritional status, lifestyle variables, and the level of genotoxicity in an exposed population and a control group...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28524757/wood-smoke-exposure-of-portuguese-wildland-firefighters-dna-and-oxidative-damage-evaluation
#16
Ana Abreu, Carla Costa, Susana Pinho E Silva, Simone Morais, Maria do Carmo Pereira, Adília Fernandes, Vanessa Moraes de Andrade, João Paulo Teixeira, Solange Costa
Portugal is among the European Union countries most devastated by forest fires each year. In the last three decades, more than 3.8 million hectares of forest were burned. Wildland firefighters are exposed to a variety of hazards, including many toxic combustion products that may lead to deleterious health effects. Epidemiological studies showed a positive association between firefighting and several chronic diseases, including cancer. Results from biomonitoring studies in firefighters, particularly concerning genotoxicity evaluation, constitute a valuable tool for investigating important occupational hazards...
May 19, 2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/28524728/a-reduction-in-dna-damage-in-neural-tissue-and-peripheral-blood-of-old-mice-treated-with-caffeine
#17
Adriani Paganini Damiani, Michelle Lima Garcez, Larissa Letieli de Abreu, Taís Helena Tavares, Carina Rodrigues Boeck, Vanessa Moraes de Andrade
Studies on caffeine consumption have shown a negative correlation with development of some diseases with subsequent beneficial manifestations. Our aim was to assess the effects of caffeine on peripheral blood and neural tissue DNA in young adult and aged mice. Male Swiss mice (age 2-3 or 16-18 months, respectively) were treated with a caffeine solution (0.3 g/l) for 4 weeks, while controls received water. After the treatments, blood and hippocampal cells (for a comet assay) and femurs (for a micronucleus [MN] test) were collected...
May 19, 2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/28524089/green-synthesized-zinc-oxide-zno-nanoparticles-induce-oxidative-stress-and-dna-damage-in-lathyrus-sativus-l-root-bioassay-system
#18
Kamal K Panda, Dambaru Golari, A Venugopal, V Mohan M Achary, Ganngam Phaomei, Narasimham L Parinandi, Hrushi K Sahu, Brahma B Panda
Zinc oxide nanoparticles (ZnONP-GS) were synthesised from the precursor zinc acetate (Zn(CH₃COO)₂) through the green route using the milky latex from milk weed (Calotropis gigantea L. R. Br) by alkaline precipitation. Formation of the ZnONP-GS was monitored by UV-visible spectroscopy followed by characterization and confirmation by energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), and X-ray diffraction (XRD). Both the ZnONP-GS and the commercially available ZnONP-S (Sigma-Aldrich) and cationic Zn(2+) from Zn(CH₃COO)₂ were tested in a dose range of 0-100 mg·L(-1) for their potency (i) to induce oxidative stress as measured by the generation reactive oxygen species (ROS: O₂(•-), H₂O₂ and (•)OH), cell death, and lipid peroxidation; (ii) to modulate the activities of antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD), guaiacol peroxidase (GPX), and ascorbate peroxidase (APX); and (iii) to cause DNA damage as determined by Comet assay in Lathyrus sativus L...
May 18, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28524082/cellular-repair-of-dna-dna-cross-links-induced-by-1-2-3-4-diepoxybutane
#19
Lisa N Chesner, Amanda Degner, Dewakar Sangaraju, Shira Yomtoubian, Susith Wickramaratne, Bhaskar Malayappan, Natalia Tretyakova, Colin Campbell
Xenobiotic-induced interstrand DNA-DNA cross-links (ICL) interfere with transcription and replication and can be converted to toxic DNA double strand breaks. In this work, we investigated cellular responses to 1,4-bis-(guan-7-yl)-2,3-butanediol (bis-N7G-BD) cross-links induced by 1,2,3,4-diepoxybutane (DEB). High pressure liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI⁺-MS/MS) assays were used to quantify the formation and repair of bis-N7G-BD cross-links in wild-type Chinese hamster lung fibroblasts (V79) and the corresponding isogenic clones V-H1 and V-H4, deficient in the XPD and FANCA genes, respectively...
May 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28521819/linking-hypoxia-dna-damage-and-proliferation-in-multicellular-tumor-spheroids
#20
Stephen Riffle, Ram Naresh Pandey, Morgan Albert, Rashmi S Hegde
BACKGROUND: Multicellular Tumor Spheroids are frequently used to mimic the regionalization of proliferation and the hypoxic environment within avascular tumors. Here we exploit these features to study the activation of DNA damage repair pathways and their correlation to developing hypoxia. METHODS: Activation of DNA damage repair markers, proliferation, cell death, glycogen accumulation and developing hypoxia were investigated using immunofluorescence, immuno-histochemistry, EdU incorporation, Western blots, COMET assays, and pharmacological agents in A673 Ewing sarcoma spheroids and monolayer cultures...
May 18, 2017: BMC Cancer
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