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https://www.readbyqxmd.com/read/29332151/1h-13c-and-15n-backbone-and-partial-side-chain-resonance-assignments-of-the-c-terminal-domain-of-hiv-1-pr55gag-encompassed-in-ncp15
#1
Valéry Larue, Marjorie Catala, Anissa Belfetmi, Loussiné Zargarian, Olivier Mauffret, Carine Tisné
During HIV-1 assembly, the Pr55Gag polyprotein precursor (Gag) interacts with the genomic RNA, with lipids of the plasma membrane, with host proteins (ALIX, TSG101) through the ESCRT complex, with the viral protein Vpr and are involved in intermolecular interactions with other Pr55Gag proteins. This network of interactions is responsible for the formation of the viral particle, the selection of genomic RNA and the packaging of Vpr. The C-terminal domain of Gag encompassed in NCp15 is involved in the majority of these interactions, either by its nucleocapsid or its p6 domains...
January 13, 2018: Biomolecular NMR Assignments
https://www.readbyqxmd.com/read/29304063/expression-of-human-immunodeficiency-virus-type-1-hiv-1-coat-protein-genes-in-plants-using-cotton-leaf-curl-multan-betasatellite-based-vector
#2
Elham Ataie Kachoie, Seyed Ali Akbar Behjatnia, Sara Kharazmi
It has already been demonstrated that a betasatellite associated with cotton leaf curl Multan virus (CLCuMB) can be used as a plant and animal gene delivery vector to plants. To examine the ability of CLCuMB as a tool to transfer coat protein genes of HIV-1 to plants, two recombinant CLCuMB constructs in which the CLCuMB βC1 ORF was replaced with two HIV-1 genes fractions including a 696 bp DNA fragment related to the HIV-1 p24 gene and a 1501 bp DNA fragment related to the HIV-1 gag gene were constructed...
2018: PloS One
https://www.readbyqxmd.com/read/29299545/colorimetric-molecular-diagnosis-of-the-hiv-gag-gene-using-dnazyme-and-a-complementary-dna-extended-primer
#3
Seong U Kim, Bhagwan S Batule, Hyoyoung Mun, Ju-Young Byun, Won-Bo Shim, Min-Gon Kim
We have developed a novel strategy for the colorimetric detection of PCR products by utilizing a target-specific primer modified at the 5'-end with an anti-DNAzyme sequence. A single-stranded DNAzyme sequence folds into a G-quadruplex structure with hemin and shows strong peroxidase activity. When the complementary strand binds to the DNAzyme sequence, it blocks the formation of the G-quadraduplex structure and loses its peroxidase activity. In the presence of the target gene, PCR amplification proceeds, and anti-DNAzyme sequence modified primers present in the reaction mixture form a double strand through primer extension...
January 4, 2018: Analyst
https://www.readbyqxmd.com/read/29296946/latent-human-cytomegalovirus-enhances-hiv-1-infection-in-cd34-progenitor-cells
#4
Allen Ka Loon Cheung, Yiru Huang, Hau Yee Kwok, Min Chen, Zhiwei Chen
Individuals who have been preinfected by human cytomegalovirus (HCMV) are more prone to AIDS disease progression after subsequent HIV-1 infection but the underlying mechanism remains elusive. HCMV is a ubiquitous DNA virus that commonly establishes lifelong latent infection in CD34+ progenitor cells, where latency-specific HCMV genes may modulate host restriction to HIV-1 infection. To test this hypothesis, we studied progenitor cells that are known to resist replicative HIV-1 infection because of the intrinsic expression of host restriction factors...
January 24, 2017: Blood Advances
https://www.readbyqxmd.com/read/29273355/what-is-the-most-important-for-elite-control-genetic-background-of-patient-genetic-background-of-partner-both-or-neither-description-of-complete-natural-history-within-a-couple-of-msm
#5
M Bendenoun, A Samri, V Avettand-Fènoël, S Cardinaud, B Descours, G Carcelain, M-C Mazeron, J-F Bergmann, A Urrutia, A Moris, C Rouzioux, F Simon, P Andre, M Pocard, X Dray, T Mourez, V Vieillard, B Autran, F Barin, P Sellier
BACKGROUND: We describe a homosexual man who strongly controlled HIV-1 for ten years despite lack of protective genetic background. METHODS: HIV-1 DNA was measured in blood and other tissues. Cell susceptibility was evaluated with various strains. HIV-1-specific (CD4 and CD8 activation markers and immune check points) and NK cells responses were assessed; KIRs haplotypes and HLA alleles were determined. FINDINGS: Two HIV-1 RNA copies/mL of plasma were detected in 2009, using an ultra-sensitive assay...
December 7, 2017: EBioMedicine
https://www.readbyqxmd.com/read/29260441/effects-of-docosahexaenoic-acid-on-locomotor-activity-in-ethanol-treated-hiv-1-transgenic-rats
#6
Jianlin He, Wenfei Huang, Shizhong Zheng, Michael Vigorito, Sulie L Chang
Binge drinking affects the onset and progression of human immunodeficiency virus (HIV)-associated neurological disorders. The HIV-1 transgenic (HIV-1Tg) rat was created with a gag- and pol-deleted HIV-1 viral genome to mimic HIV-infected patients receiving combination anti-retroviral therapy (cART). Docosahexaenoic acid (DHA) is a marine compound that modulates inflammatory responses. Using HIV-1Tg rats subjected to binge exposure to ethanol (EtOH), this study examined whether DHA could reduce the detrimental neurological effects of EtOH and HIV proteins...
December 19, 2017: Journal of Neurovirology
https://www.readbyqxmd.com/read/29258320/characterization-of-a-novel-hiv-1-circulating-recombinant-form-crf01_ae-b-c-crf96_cpx-in-yunnan-china
#7
Jing Miao, Jieyu Ran, Yindi Song, Yang Liu, Li Gao, Zhijiang Miao, Chunyue Zhang, Yue Feng, Xueshan Xia
Currently, complex HIV-1 recombination among the B', C, and CRF01_AE genotypes have frequently arisen in Yunnan, China. A novel HIV-1 complex circulating recombinant form (CRF) consisting of B', C, and CRF01_AE (CRF96_cpx) was recently characterized from three epidemiologically unlinked individuals. Two strains of them were isolated from the injecting drug users in this study, the remaining one strain (JL. RL01) obtained from a previous report in Jilin province. Phylogenetic analysis based on near full-length genome (NFLG) revealed that CRF96_cpx formed a distinct monophyletic cluster supported by a high bootstrap value of 100%, distantly related to all known HIV-1 subtypes/CRFs...
December 19, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/29246070/propensity-of-a-single-walled-carbon-nanotube-peptide-to-mimic-a-kk10-peptide-in-an-hla-tcr-complex
#8
Mei Feng, David R Bell, Ruhong Zhou
The application of nanotechnology to improve disease diagnosis, treatment, monitoring, and prevention is the goal of nanomedicine. We report here a theoretical study of a functionalized single-walled carbon nanotube (CNT) mimic binding to a human leukocyte antigen-T cell receptor (HLA-TCR) immune complex as a first attempt of a potential nanomedicine for human immunodeficiency virus (HIV) vaccine development. The carbon nanotube was coated with three arginine residues to imitate the HIV type 1 immunodominant viral peptide KK10 (gag 263-272: KRWIILGLNK), named CNT-peptide hereafter...
December 14, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29239896/ex-vivo-antiretroviral-potency-of-newer-integrase-strand-transfer-inhibitors-cabotegravir-and-bictegravir-in-hiv-1-non-b-subtypes
#9
Ujjwal Neogi, Kamlendra Singh, Shambhu G Aralaguppe, Leonard C Rogers, Duncan T Njenda, Stefan G Sarafianos, Bo Hejdeman, Anders Sönnerborg
OBJECTIVE: To determine the antiretroviral activity of the integrase strand transfer inhibitors (INSTIs), raltegravir, elvitegravir, dolutegravir, cabotegravir and bictegravir, against different subtypes as well as primary and acquired drug resistance mutations (DRM) in a patient-cohort infected with diverse subtypes. DESIGN: Biochemical and virological drug sensitivity analysis using patient derived HIV-1 genes and cross-sectional/longitudinal clinical study. METHODS: Assays for inhibition of 3'-end processing (IC50-3P), strand transfer (IC50-ST) and drug sensitivity (DSA) for five INSTIs were done using patient-derived integrase or gag-pol genes from subtypes A1, B, C, 01_AE and 02_AG...
December 12, 2017: AIDS
https://www.readbyqxmd.com/read/29237328/modeling-the-full-length-hiv-1-gag-polyprotein-reveals-the-role-of-its-p6-subunit-in-viral-maturation-and-the-effect-of-non-cleavage-site-mutations-in-protease-drug-resistance
#10
Chinh Tran-To Su, Chee-Keong Kwoh, Chandra Shekhar Verma, Samuel Ken-En Gan
HIV polyprotein Gag is increasingly found to contribute to protease inhibitor resistance. Despite its role in viral maturation and in developing drug resistance, there remain gaps in the knowledge of the role of certain Gag subunits (e.g. p6), and that of non-cleavage mutations in drug resistance. As p6 is flexible, it poses a problem for structural experiments, and is hence often omitted in experimental Gag structural studies. Nonetheless, as p6 is an indispensable component for viral assembly and maturation, we have modeled the full length Gag structure based on several experimentally determined constraints and studied its structural dynamics...
December 13, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29226692/identification-of-a-novel-hiv-1-unique-recombinant-form-between-b-crf01_ae-and-crf07_bc-in-men-who-have-sex-with-men-in-guangxi-china
#11
Desheng Kong, Yan Wang, Chen Wang, Shujia Liang, Yi Feng, Yiming Shao, Liying Ma
B, CRF01_AE and CRF07_BC are three major HIV-1 subtypes circulating in China. Here we detected a novel CCR5-tropic HIV-1 recombinant virus (GX2014EU23), which was isolated from an HIV-1-infected man who had sex with men (MSM) in Guangxi, China. Phylogenetic analysis of the near full-length genome (NFLG) showed that GX2014EU23 was consisted of at least 7 segments, including two B, two CRF01_AE and three CRF07_BC segments. Recombinant breakpoints of GX2014EU23 were observed in the gag, pol, rev and env regions...
December 10, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/29226013/novel-therapeutic-approach-for-inhibition-of-hiv-1-using-cell-penetrating-peptide-and-bacterial-toxins
#12
Steven Samuels, Zainab Alwan, Marceline Egnin, Jessie Jaynes, Terry D Connell, Gregory C Bernard, Toufic Nashar
Despite advancements in our understanding of HIV-1 pathogenesis, critical virus components for immunity, vaccines trials, and drugs development, challenges remain in the fight against HIV-1. Of great importance is the inhibitory function of microbicidal cell penetrating peptides and bacterial toxins that interfere with production and neutralize infection of HIV-1 particles. We demonstrate that the neutralizing activity of a cationic 18 amino acids peptide, is similar to a broadly neutralizing human antibody, and inhibits production of two HIV-1 strains in human cell lines...
October 2017: Journal of AIDS & Clinical Research
https://www.readbyqxmd.com/read/29220350/deep-sequencing-of-near-full-length-hiv-1-genomes-from-plasma-identifies-circulating-subtype-c-and-infrequent-occurrence-of-ac-recombinant-form-in-southern-india
#13
Shuba Varshini Alampalli, Michael M Thomson, Raghavan Sampathkumar, Karthi Sivaraman, Anto Jesuraj U K J, Chirag Dhar, George D Souza, Neil Berry, Annapurna Vyakarnam
India has the third largest number of HIV-1-infected individuals accounting for approximately 2.1 million people, with a predominance of circulating subtype C strains and a low prevalence of subtype A and A1C and BC recombinant forms, identified over the past two decades. Recovery of near full-length HIV-1 genomes from a plasma source coupled with advances in next generation sequencing (NGS) technologies and development of universal methods for amplifying whole genomes of HIV-1 circulating in a target geography or population provides the opportunity for a detailed analysis of HIV-1 strain identification, evolution and dynamics...
2017: PloS One
https://www.readbyqxmd.com/read/29212942/factors-leading-to-the-loss-of-natural-elite-control-of-hiv-1-infection
#14
María Pernas, Laura Tarancón-Diez, Esther Rodríguez-Gallego, Josep Gómez, Julia G Prado, Concepción Casado, Beatriz Dominguez-Molina, Isabel Olivares, Maite Coiras, Agathe León, Carmen Rodriguez, Jose Miguel Benito, Norma Rallón, Montserrat Plana, Onofre Martinez-Madrid, Marta Dapena, Jose Antonio Iribarren, Jorge Del Romero, Felipe García, José Alcamí, M Ángeles Muñoz-Fernández, Francisco Vidal, Manuel Leal, Cecilio Lopez-Galindez, Ezequiel Ruiz-Mateos
HIV-1 elite controllers (EC) maintain undetectable viral load (VL) in the absence of antiretroviral treatment. However, these subjects have heterogeneous clinical outcomes including a proportion loosing HIV-1 control over time. In this work we compared, in a longitudinal design, transient EC, analyzed before and after the loss of virological control, versus persistent EC. The aim was to identify factors leading to the loss of natural virological control of HIV-1-infection with a longitudinal retrospective study design...
December 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29209312/dual-molecular-mechanisms-govern-escape-at-immunodominant-hla-a2-restricted-hiv-epitope
#15
David K Cole, Anna Fuller, Garry Dolton, Efthalia Zervoudi, Mateusz Legut, Kim Miles, Lori Blanchfield, Florian Madura, Christopher J Holland, Anna M Bulek, John S Bridgeman, John J Miles, Andrea J A Schauenburg, Konrad Beck, Brian D Evavold, Pierre J Rizkallah, Andrew K Sewell
Serial accumulation of mutations to fixation in the SLYNTVATL (SL9) immunodominant, HIV p17 Gag-derived, HLA A2-restricted cytotoxic T lymphocyte epitope produce the SLFNTIAVL triple mutant "ultimate" escape variant. These mutations in solvent-exposed residues are believed to interfere with TCR recognition, although confirmation has awaited structural verification. Here, we solved a TCR co-complex structure with SL9 and the triple escape mutant to determine the mechanism of immune escape in this eminent system...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29208937/elucidating-the-in-vivo-interactome-of-hiv-1-rna-by-hybridization-capture-and-mass-spectrometry
#16
Rachel A Knoener, Jordan T Becker, Mark Scalf, Nathan M Sherer, Lloyd M Smith
HIV-1 replication requires myriad interactions between cellular proteins and the viral unspliced RNA. These interactions are important in archetypal RNA processes such as transcription and translation as well as for more specialized functions including alternative splicing and packaging of unspliced genomic RNA into virions. We present here a hybridization capture strategy for purification of unspliced full-length HIV RNA-protein complexes preserved in vivo by formaldehyde crosslinking, and coupled with mass spectrometry to identify HIV RNA-protein interactors in HIV-1 infected cells...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29205929/dendritic-cell-targeted-hiv-1-gag-protein-vaccine-provides-help-to-a-recombinant-newcastle-disease-virus-vectored-vaccine-including-mobilization-of-protective-cd8-t-cells
#17
Loveline N Ngu, Nadesh N Nji, Georgia Ambada, Apeh A Ngoh, Ghislain D Njambe Priso, Jules C Tchadji, Abel Lissom, Suzanne H Magagoum, Carol N Sake, Thibau F Tchouangueu, George O Chukwuma, Arinze S Okoli, Bertrand Sagnia, Rebecca Chukwuanukwu, Denis M Tebit, Charles O Esimone, Alain B Waffo, Chae G Park, Klaus Überla, Godwin W Nchinda
INTRODUCTION: Recombinant Newcastle Disease virus (rNDV) vectored vaccines are safe mucosal applicable vaccines with intrinsic immune-modulatory properties for the induction of efficient immunity. Like all viral vectored vaccines repeated inoculation via mucosal routes invariably results to immunity against viral vaccine vectors. To obviate immunity against viral vaccine vectors and improve the ability of rNDV vectored vaccines in inducing T cell immunity in murine air way we have directed dendritic cell targeted HIV-1 gag protein (DEC-Gag) vaccine; for the induction of helper CD4+ T cells to a Recombinant Newcastle disease virus expressing codon optimized HIV-1 Gag P55 (rNDV-L-Gag) vaccine...
December 4, 2017: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/29192235/multiplex-single-cell-visualization-of-nucleic-acids-and-protein-during-hiv-infection
#18
Maritza Puray-Chavez, Philip R Tedbury, Andrew D Huber, Obiaara B Ukah, Vincent Yapo, Dandan Liu, Juan Ji, Jennifer J Wolf, Alan N Engelman, Stefan G Sarafianos
Technical limitations in simultaneous microscopic visualization of RNA, DNA, and proteins of HIV have curtailed progress in this field. To address this need we develop a microscopy approach, multiplex immunofluorescent cell-based detection of DNA, RNA and Protein (MICDDRP), which is based on branched DNA in situ hybridization technology. MICDDRP enables simultaneous single-cell visualization of HIV (a) spliced and unspliced RNA, (b) cytoplasmic and nuclear DNA, and (c) Gag. We use MICDDRP to visualize incoming capsid cores containing RNA and/or nascent DNA and follow reverse transcription kinetics...
December 1, 2017: Nature Communications
https://www.readbyqxmd.com/read/29186704/crf35-ad-as-the-main-circulating-genotype-of-human-immunodeficiency-virus-type-1-infection-in-iran-a-phylogenetic-and-demographic-based-study
#19
Sayed-Hamidreza Mozhgani, Seyedeh Arefeh Ebrahimian, Hoda Gudarzi, Seyed Mohammad Jazayeri, Fatemeh Jahanbakhsh, Minoo Mohraz, Seyed Abdolrahim Rezaee, Kayhan Azadmanesh, Saber Soltani, Mehdi Norouzi
Finding the predominant circulating subtype of human immunodeficiency virus type 1 (HIV-1) and surveying co-infection with other infectious viruses are crucial to making preventive decisions. To this end, 50 Iranian HIV-positive patients made up of 37 men and 13 women were selected. Most of the HIV-positive patients (70%) were intravenous drug users (IDUs), and 48 and 32% of patients were co-infected with HCV and HBV, respectively. The rate of simultaneous infection with HIV, HCV, and HBV was found to be 6%...
November 30, 2017: Intervirology
https://www.readbyqxmd.com/read/29184023/effects-of-mutations-on-replicative-fitness-and-major-histocompatibility-complex-class-i-binding-affinity-are-among-the-determinants-underlying-cytotoxic-t-lymphocyte-escape-of-hiv-1-gag-epitopes
#20
Yushen Du, Tian-Hao Zhang, Lei Dai, Xiaojuan Zheng, Aleksandr M Gorin, John Oishi, Ting-Ting Wu, Janice M Yoshizawa, Xinmin Li, Otto O Yang, Otoniel Martinez-Maza, Roger Detels, Ren Sun
Certain "protective" major histocompatibility complex class I (MHC-I) alleles, such as B*57 and B*27, are associated with long-term control of HIV-1 in vivo mediated by the CD8+ cytotoxic-T-lymphocyte (CTL) response. However, the mechanism of such superior protection is not fully understood. Here we combined high-throughput fitness profiling of mutations in HIV-1 Gag, in silico prediction of MHC-peptide binding affinity, and analysis of intraperson virus evolution to systematically compare differences with respect to CTL escape mutations between epitopes targeted by protective MHC-I alleles and those targeted by nonprotective MHC-I alleles...
November 28, 2017: MBio
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