keyword
MENU ▼
Read by QxMD icon Read
search

Hiv gag

keyword
https://www.readbyqxmd.com/read/28431010/clinical-trial-of-the-anti-pd-l1-antibody-bms-936559-in-hiv-1-infected-participants-on-suppressive-antiretroviral-therapy
#1
Cynthia L Gay, Ronald J Bosch, Justin Ritz, Jason M Hataye, Evgenia Aga, Randall L Tressler, Stephen W Mason, Carey K Hwang, Dennis M Grasela, Neelanjana Ray, Josh C Cyktor, John M Coffin, Edward P Acosta, Richard A Koup, John W Mellors, Joseph J Eron
Background.: Reversing immune exhaustion with an anti-PD-L1 antibody may improve HIV-1-specific immunity and increase clearance of HIV-1 expressing cells. Methods.: Phase I, randomized, double-blind, placebo-controlled dose-escalating study of BMS-936559including HIV-1-infected adults ≥18 to ≤70 years on suppressive antiretroviral therapy with CD4+ counts ≥350 cells/μL and detectable plasma HIV-1 RNA by single copy assay. Data on single infusions of BMS-936559 (0...
April 18, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28424286/subtype-specific-differences-in-gag-protease-driven-replication-capacity-are-consistent-with-inter-subtype-differences-in-hiv-1-disease-progression
#2
Marion W Kiguoya, Jaclyn K Mann, Denis Chopera, Kamini Gounder, Guinevere Q Lee, Peter W Hunt, Jeffrey N Martin, T Blake Ball, Joshua Kimani, Zabrina L Brumme, Mark A Brockman, Thumbi Ndung'u
There are marked differences in the spread and prevalence of HIV-1 subtypes worldwide, and differences in clinical progression have been reported. However, the biological reasons underlying these differences are unknown. Gag-protease is essential for HIV-1 replication and Gag-protease-driven replication capacity has previously been correlated with disease progression. We show that Gag-protease replication capacity correlates significantly with that of whole isolates (r=0.51; p=0.04), indicating that Gag-protease is a significant contributor to viral replication capacity...
April 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28424281/distinct-roles-of-vaccinia-virus-nf-kb-inhibitor-proteins-a52-b15-and-k7-in-the-immune-response
#3
Mauro Di Pilato, Ernesto Mejías-Pérez, Carlos Oscar S Sorzano, Mariano Esteban
Poxviruses use a complex strategy to escape immune control, by expressing immunomodulatory proteins that could limit their use as vaccine vectors. To test the role of poxvirus NF-κB pathway inhibitors A52, B15 and K7 in immunity, we deleted their genes in a NYVAC vaccinia virus strain that expresses HIV-1 clade C antigens. After infection of mice, ablation of A52R, B15R and K7R increased dendritic cell, natural killer cell and neutrophil migration as well as chemokine/cytokine expression. Revertant viruses for these genes confirmed their role in inhibiting the innate immune system...
April 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28422791/intrastructural-help-improving-the-hiv-1-envelope-antibody-response-induced-by-virus-like-particle-vaccines
#4
Vladimir Temchura, Klaus Überla
PURPOSE OF REVIEW: The importance of IgG Fc-effector functions for the efficacy of HIV vaccines is increasingly recognized. Although different types of vaccines were shown to induce antibodies with different Fc-activities, there is no clear strategy how to raise antibody responses with a desired pattern of Fc-effector functions. Given the central role of T-helper cells in regulating the germinal center reaction and the differentiation of B cells in an antigen-specific manner, the review will discuss whether T-helper cells directed against non-HIV envelope (Env) antigens could be harnessed to improve the HIV-Env antibody response...
May 2017: Current Opinion in HIV and AIDS
https://www.readbyqxmd.com/read/28388673/proteolysis-of-mature-hiv-1-p6-gag-protein-by-the-insulin-degrading-enzyme-ide-regulates-virus-replication-in-an-env-dependent-manner
#5
Friedrich Hahn, Adrian Schmalen, Christian Setz, Melanie Friedrich, Stefan Schlößer, Julia Kölle, Robert Spranger, Pia Rauch, Kirsten Fraedrich, Tatjana Reif, Julia Karius-Fischer, Ashok Balasubramanyam, Petra Henklein, Torgils Fossen, Ulrich Schubert
There is a significantly higher risk for type II diabetes in HIV-1 carriers, albeit the molecular mechanism for this HIV-related pathology remains enigmatic. The 52 amino acid HIV-1 p6 Gag protein is synthesized as the C-terminal part of the Gag polyprotein Pr55. In this context, p6 promotes virus release by its two late (L-) domains, and facilitates the incorporation of the viral accessory protein Vpr. However, the function of p6 in its mature form, after proteolytic release from Gag, has not been investigated yet...
2017: PloS One
https://www.readbyqxmd.com/read/28374250/human-rhinovirus-a1-as-an-expression-vector
#6
Khamis Tomusange, Danushka Wijesundara, Eric James Gowans, Branka Grubor-Bauk
Expression vectors that are based on live human rhinoviruses (HRVs) are attractive, yet often overlooked in vaccine development due to their limited capacity for foreign gene inserts and poor genetic stability. This chapter describes a novel methodology to engineer a replication-competent genetically stable recombinant HRV (rHRV) without affecting viral replication capability. We have previously used these methods to generate live, genetically stable recombinant HRVs encoding HIV Gag and Tat proteins (rHRV-Gag-Tat), a potential mucosally targeted HIV vaccine...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28372798/stimulation-of-hiv-specific-t-cell-clonotypes-using-allogeneic-hla
#7
Coral-Ann Almeida, Paula van Miert, Kane O'Driscoll, Yvonne M Zoet, Abha Chopra, Mark Watson, Dianne de Santis, Campbell Witt, Mina John, Frans H J Claas, Lloyd J D'Orsogna
We hypothesized that HIV-specific CD8 T cell clonotypes can be stimulated by allogeneic HLA molecules. Multiple HIV-specific CD8 T cell clones were derived from 12 individuals with chronic HIV infection, specific for 13 different HIV Gag antigens and restricted to 7 different HLA molecules. The generated T cell clones were assayed for alloreactivity against a panel of single HLA class I expressing cell lines (SALs). HIV-specific T cells recognising at least one allogeneic HLA molecule could be identified from 7 of 12 patients tested...
March 28, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28372592/a-comparative-analysis-of-the-foamy-and-ortho-virus-capsid-structures-reveals-an-ancient-domain-duplication
#8
William R Taylor, Jonathan P Stoye, Ian A Taylor
BACKGROUND: The Spumaretrovirinae (foamy viruses) and the Orthoretrovirinae (e.g. HIV) share many similarities both in genome structure and the sequences of the core viral encoded proteins, such as the aspartyl protease and reverse transcriptase. Similarity in the gag region of the genome is less obvious at the sequence level but has been illuminated by the recent solution of the foamy virus capsid (CA) structure. This revealed a clear structural similarity to the orthoretrovirus capsids but with marked differences that left uncertainty in the relationship between the two domains that comprise the structure...
April 4, 2017: BMC Structural Biology
https://www.readbyqxmd.com/read/28369211/antiviral-activity-safety-and-exposure-response-relationships-of-gsk3532795-a-second-generation-hiv-1-maturation-inhibitor-administered-as-monotherapy-or-in-combination-with-atazanavir%C3%A2-ritonavir-in-a-phase-2a-randomized-dose-ranging-controlled-trial-ai468002
#9
Carey Hwang, Dirk Schürmann, Christian Sobotha, Marta Boffito, Heather Sevinsky, Neelanjana Ray, Palanikumar Ravindran, Hong Xiao, Christian Keicher, Andreas Hüser, Mark Krystal, Ira B Dicker, Dennis Grasela, Max Lataillade
BACKGROUND: GSK3532795 is a second-generation human immunodeficiency virus (HIV-1) maturation inhibitor that targets HIV-1 Gag, inhibiting the final protease cleavage between capsid protein p24 and spacer protein-1, producing immature, non-infectious virions. METHODS: AI468002 (NCT01803074): Phase 2a, randomized, dosing-ranging multipart trial. In Part A, subtype B-infected subjects received 5 to 120 mg GSK3532795 (or placebo) once daily (QD) for 10 days. In Part B, subtype B-infected subjects received 40 mg or 80mg GSK3532795 QD with atazanavir (ATV) ± ritonavir (RTV) or standard-of-care (SOC; tenofovir disoproxil fumarate 300 mg, emtricitabine 200 mg, and ATV/RTV 300 mg/100 mg) for 28 days...
March 23, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28358392/analyzing-trapped-protein-complexes-by-virotrap-and-sfinx
#10
Kevin Titeca, Emmy Van Quickelberghe, Noortje Samyn, Delphine De Sutter, Annick Verhee, Kris Gevaert, Jan Tavernier, Sven Eyckerman
The analysis of protein interaction networks is one of the key challenges in the study of biology. It connects genotypes to phenotypes, and disruption of such networks is associated with many pathologies. Virtually all the approaches to the study of protein complexes require cell lysis, a dramatic step that obliterates cellular integrity and profoundly affects protein interactions. This protocol starts with Virotrap, a novel approach that avoids the need for cell homogenization by fusing the protein of interest to the HIV-1 Gag protein, trapping protein complexes in virus-like particles...
May 2017: Nature Protocols
https://www.readbyqxmd.com/read/28352266/canonical-and-cross-reactive-binding-of-nk-cell-inhibitory-receptors-to-hla-c-allotypes-is-dictated-by-peptides-bound-to-hla-c
#11
Malcolm J W Sim, Stacy A Malaker, Ayesha Khan, Janet M Stowell, Jeffrey Shabanowitz, Mary E Peterson, Sumati Rajagopalan, Donald F Hunt, Daniel M Altmann, Eric O Long, Rosemary J Boyton
BACKGROUND: Human natural killer (NK) cell activity is regulated by a family of killer cell immunoglobulin-like receptors (KIRs) that bind human leukocyte antigen (HLA) class I. Combinations of KIR and HLA genotypes are associated with disease, including susceptibility to viral infection and disorders of pregnancy. KIR2DL1 binds HLA-C alleles of group C2 (Lys(80)). KIR2DL2 and KIR2DL3 bind HLA-C alleles of group C1 (Asn(80)). However, this model cannot explain HLA-C allelic effects in disease or the impact of HLA-bound peptides...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28342872/covalent-conjugation-of-the-equine-infectious-anemia-virus-gag-with-sumo
#12
Jinzhong Wang, Shuping Wen, Rui Zhao, Jing Qi, Zhao Liu, Weiwei Li, Jing An, Charles Wood, Ying Wang
The conjugation of small ubiquitin-like modifier (SUMO) to the target protein, namely, SUMOylation, is involved in the regulation of many important biological events including host-pathogen interaction. Some viruses have evolved to exploit the host SUMOylation machinery to modify their own protein. Retroviral Gag protein plays critical roles in the viral life cycle. The HIV-1 p6 and the Moloney murine leukemia virus CA have been reported to be conjugated with SUMO. In this study, we report for the first time, to our knowledge, the covalent conjugation of equine infectious anemia virus (EIAV) Gag with SUMO...
March 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28333940/distinct-transcriptome-profiles-of-gag-specific-cd8-t-cells-temporally-correlated-with-the-protection-elicited-by-siv%C3%AE-nef-live-attenuated-vaccine
#13
Wuxun Lu, Yanmin Wan, Fangrui Ma, R Paul Johnson, Qingsheng Li
The live attenuated vaccine (LAV) SIVmac239Δnef (SIVΔnef) confers the best protection among all the vaccine modalities tested in rhesus macaque model of HIV-1 infection. This vaccine has a unique feature of time-dependent protection: macaques are not protected at 3-5 weeks post vaccination (WPV), whereas immune protection emerges between 15 and 20 WPV. Although the exact mechanisms of the time-dependent protection remain incompletely understood, studies suggested that both cellular and humoral immunities contribute to this time-dependent protection...
2017: PloS One
https://www.readbyqxmd.com/read/28318765/in-vivo-electroporation-in-dna-vlp-prime-boost-preferentially-enhances-hiv-1-envelope-specific-igg2a-neutralizing-antibody-and-cd8-t-cell-responses
#14
Xun Huang, Qianqian Zhu, Xiaoxing Huang, Lifei Yang, Yufeng Song, Ping Zhu, Paul Zhou
Although in vivo electroporation (EP) has been utilized to improve immunogenicity in DNA vaccines alone or in prime-boost regimens with both proteins and viral-vectors, no studies on in vivo EP in DNA-VLP prime-boost regimens against HIV-1 have been reported. Previously we developed stably transfected Drosophila S2 clones to produce HIV-1 virus-like particles (VLP) and demonstrated that priming mice twice with DNA plasmids encoding HIV-1 gp120 and gag and boosting twice with HIV-1 VLP (i.e. DDVV immunization) elicited both envelope-specific antibody and envelope and gag-specific CD8 T cell responses...
March 16, 2017: Vaccine
https://www.readbyqxmd.com/read/28303602/agreement-between-an-in-house-replication-competent-and-a-reference-replication-defective-recombinant-virus-assay-for-measuring-phenotypic-resistance-to-hiv-1-protease-reverse-transcriptase-and-integrase-inhibitors
#15
Francesco Saladini, Alessia Giannini, Adele Boccuto, Ilaria Vicenti, Maurizio Zazzi
BACKGROUND: Although clinical management of drug resistance is routinely based on genotypic methods, phenotypic assays remain necessary for the characterization of novel HIV-1 inhibitors, particularly against common drug-resistant variants. We describe the development and assessment of the performance of a recombinant virus assay for measuring HIV-1 susceptibility to protease (PR), reverse transcriptase (RT), and integrase (IN) inhibitors. METHODS: The system is based on the creation of replication-competent chimeric viruses through homologous recombination between patient or laboratory virus-derived PCR fragments and the corresponding NL4-3 vector where the whole Gag-PR, RT-RNaseH or IN coding regions has been deleted through inverse PCR...
March 17, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28300196/recombinant-mycobacterium-smegmatis-with-a-pmyong2-vector-expressing-human-immunodeficiency-virus-type-i-gag-can-induce-enhanced-virus-specific-immune-responses
#16
Byoung-Jun Kim, Jeong-Ryeol Gong, Ga-Na Kim, Bo-Ram Kim, So-Young Lee, Yoon-Hoh Kook, Bum-Joon Kim
Recently, we have developed a novel Mycobacterium-Escherichia coli shuttle vector system using pMyong2, which can provide an enhanced expression of heterologous genes in recombinant Mycobacterium smegmatis (rSmeg). To investigate the usefulness of rSmeg using pMyong2 in vaccine application, we vaccinated M. smegmatis with pMyong2 system expressing Human Immunodeficiency Virus Type I (HIV-1) Gag p24 antigen (rSmeg-pMyong2-p24) into mice and examined its cellular and humoral immune responses against HIV gag protein...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28298145/genotypic-characterization-of-human-immunodeficiency-virus-type-1-derived-from-antiretroviral-drug-treated-individuals-residing-in-earthquake-affected-areas-in-nepal
#17
Bharat Singh Negi, Tomohiro Kotaki, Sunil Kumar Joshi, Anup Bastola, Minato Nakazawa, Masanori Kameoka
Molecular epidemiological data on human immunodeficiency virus type 1 (HIV-1) are limited in Nepal and have not been available in areas affected by the April 2015 earthquake. Therefore, we conducted a genotypic study on HIV-1 genes derived from individuals on antiretroviral therapy residing in 14 districts in Nepal highly affected by the earthquake. HIV-1 genomic fragments were amplified from 40 blood samples of HIV treatment-failure individuals, and a sequencing analysis was performed on these genes. In the 40 samples, 29 protease, 32 reverse transcriptase, 25 gag, and 21 env genes were sequenced...
April 10, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/28292714/combined-monte-carlo-torsion-angle-molecular-dynamics-for-ensemble-modeling-of-proteins-nucleic-acids-and-carbohydrates
#18
Weihong Zhang, Steven C Howell, David W Wright, Andrew Heindel, Xiangyun Qiu, Jianhan Chen, Joseph E Curtis
We describe a general method to use Monte Carlo simulation followed by torsion-angle molecular dynamics simulations to create ensembles of structures to model a wide variety of soft-matter biological systems. Our particular emphasis is focused on modeling low-resolution small-angle scattering and reflectivity structural data. We provide examples of this method applied to HIV-1 Gag protein and derived fragment proteins, TraI protein, linear B-DNA, a nucleosome core particle, and a glycosylated monoclonal antibody...
May 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28287833/identification-of-a-novel-hiv-type-1-crf01_ae-b-c-recombinant-isolate-in-sichuan-china
#19
Yan Wang, Desheng Kong, Weisi Xu, Fan Li, Shu Liang, Yi Feng, Fujie Zhang, Yiming Shao, Liying Ma
We report in this study a novel HIV-1 unique recombinant virus (XC2014EU01) isolated from an HIV-positive man who infected through heterosexual sex in Sichuan, China. The near full-length genome analyses showed that XC2014EU01 harbored one subtype B segment in pol region and two subtype C segments in gag-pol region in a CRF01_AE backbone. The unique mosaic structure was distinctly different from the other CRF01_AE/B'/C recombinant forms reported. Phylogenetic tree analyses revealed that the subtype B region originated from a Thailand subtype B' lineage, the subtype C regions were from an India C lineage, and the backbone was from CRF01_AE...
March 13, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/28286236/5-hydroxytyrosol-inhibits-hiv-1-replication-in-primary-cells-of-the-lower-and-upper-female-reproductive-tract
#20
Elisa Saba, Paola Panina-Bordignon, Isabel Pagani, Massimo Origoni, Massimo Candiani, Claudio Doglioni, Gianluca Taccagni, Silvia Ghezzi, José Alcami, Elisa Vicenzi, Guido Poli
We investigated the potential anti-HIV-1 activity of the candidate microbicide 5-hydroxytyrosol (5-HT) both in primary human cervical tissue explants (CTE), established from tissues of women undergoing histerectomy, and in endometrium-associated leukocytes (EAL). CTE were exposed to either the laboratory-adapted HIV-1BaL or to primary viral isolates in the presence or absence of 5-HT or 3TC/lamivudine as control and were then monitored for 12 days in terms of HIV-1 p24 Gag antigen production in culture supernatants...
June 2017: Antiviral Research
keyword
keyword
67946
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"