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Pertussis fermentation culture

Thakur Datta, Devender Sharma, Inderjeet Gandhoke, Shashi Khare, Arun Bhardwaj
Since introduction of the pertussis vaccine in 1940's the morbidity and mortality due to the infection has been markedly reduced all over the world. However the adverse effects of the inactivated whole cell pertussis vaccine like pain, swelling at the site of injection, fever, vomiting anorexia, persistent crying & drowsiness have been the cause of great concern, till date. Also the safety concerns over the use of thiomersal as an inactivating agent as well preservative have been raised in the recent past. Studies in many countries have been initiated to reduce or replace thiomersal & using other inactivating agents in the vaccines...
September 2011: Journal of Communicable Diseases
Hector Budman, Nilesh Patel, Melih Tamer, Walid Al-Gherwi
A mathematical model based on a dynamic metabolic flux balance (DMFB) is developed for a process of fed-batch fermentation of Bordetella pertussis. The model is based on the maximization of growth rate at each time interval subject to stoichiometric constraints. The model is calibrated and verified with experimental data obtained in two different bioreactor experimental systems. It was found that the model calibration was mostly sensitive to the consumption or production rates of tyrosine and, for high supplementation rates, to the consumption rate of glutamate...
March 2013: Biotechnology Progress
Elen A Perpetuo, Ivo Lebrun, Luis Juliano, Maria Aparecida Juliano, Maria Aparecida Sakauchi, Sally M A Prado
Proteases were identified and characterized from the culture supernatant of the C. diphtheriae and B. pertussis bacteria. The proteases were secreted in the media and detected at the end of the exponential growth phase. Activity was detected in some fluorescent substrates, based on selected protein sequences such as insuline beta-chain, bradykinin, and synaptobrevin. The proteases were purified by means of gel filtration chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the purified proteins indicated, for the main secreted proteins, an estimated molecular mass of 30 kDa in C...
2007: Preparative Biochemistry & Biotechnology
J A Bogdan, J Nazario-Larrieu, J Sarwar, P Alexander, M S Blake
Pertussis toxin (Ptx) expression and secretion in Bordetella pertussis are regulated by a two-component signal transduction system encoded by the bvg regulatory locus. However, it is not known whether the metabolic pathways and growth state of the bacterium influence synthesis and secretion of Ptx and other virulence factors. We have observed a reduction in the concentration of Ptx per optical density unit midway in fermentation. Studies were conducted to identify possible factors causing this reduction and to develop culture conditions that optimize Ptx expression...
November 2001: Infection and Immunity
L V Miriasova, I A Basnak'ian, T V Kopaeva, I Iu Sarkisov
The processes of the cultivation of Bordetella pertussis, immobilized on polyurethane carrier in a fermenter, were carried out and studied. Acellular pertussis preparations were produced from the culture fluid obtained in the batch and multi-cycle cultivation processes with immobilized cells, as well as in the process with interrupted fermentation (for confirming the possibility of the preservation of cell viability). The content of protein and B. pertussis toxin in these preparations, as well as their leukocytes-stimulating and hemagglutinating activity, did not differ from similar characteristics of preparations obtained from culture fluid in homogeneous cultivation...
May 1999: Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii
J Germán Santiago, N Zamora, E de la Rosa, C Alba Carrión, P Padrón, M Hernández, M Betancourt, N Moretti
The production of Pertussis Vaccine was reevaluated at the Instituto Nacional de Higiene "Rafael Rangel" in order to optimise it in terms of vaccine yield, potency, specific toxicity and efficiency (cost per doses). Four different processes, using two culture media (Cohen-Wheeler and Fermentación Glutamato Prolina-1) and two types of bioreactors (25 L Fermentador Caracas and a 450 L industrial fermentor) were compared. Runs were started from freeze-dried strains (134 or 509) and continued until the obtention of the maximal yield...
1995: Acta Científica Venezolana
O Galicia, H Villalva, A Pérez, S Pérez, A Medina
No abstract text is available yet for this article.
November 1981: Salud Pública de México
A Medina García, T A Martín Escobar, E T Reyes
No abstract text is available yet for this article.
November 1981: Salud Pública de México
L Recamier, A Medina, H Rodríguez
No abstract text is available yet for this article.
November 1981: Salud Pública de México
A Medina García, I Gómez Reyes
No abstract text is available yet for this article.
November 1981: Salud Pública de México
J Bellalou, E H Relyveld
Conditions for growing Bordetella pertussis bacteria in fermentors were studied for the purpose of producing highly protective vaccines. Bacteria with high protective potency were obtained in fermentors when the inoculum consisted of a dense suspension of young B. pertussis cells grown on Bordet-Gengou agar plates. When the time of cultivation in fermentors exceeded 30 h, bacteria harvested showed lower protective potency activity and lower levels of histamine sensitizing factor (HSF) and leukocytosis promoting factor (LPF), indicating that the optimal time for collection is at the end of the logarithmic growth phase...
July 1984: Annales de Microbiologie
D W Stainer, M J Scholte
No abstract text is available yet for this article.
October 1970: Journal of General Microbiology
J Cameron, P Rousseau, G Trepanier
No abstract text is available yet for this article.
January 1985: Journal of Biological Standardization
P Chong, M Klein
A general procedure for purifying biologically active pertussis toxin from Bordetella pertussis fermentation broth using affinity chromatography on heat-treated fetuin-Sepharose CL-4B is described. Diethanolamine is used as eluent in this single-step purification to prepare endotoxin-free pertussis toxin in good yield (70%) and high purity (greater than 95%). This one-step affinity chromatography procedure can be easily applied for large-scale preparation of pertussis toxin S1 subunit and its B-component. The affinity-purified S1 subunit is devoid of any of the histamine-sensitizing activity normally associated with pertussis toxin...
July 1989: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
M A Romanos, J J Clare, K M Beesley, F B Rayment, S P Ballantine, A J Makoff, G Dougan, N F Fairweather, I G Charles
Acellular whooping cough vaccines are based on pertussis toxoid but their effectiveness may be increased by the addition of other Bordetella pertussis antigens. We expressed the immunogenic outer membrane protein pertactin (P69) from B. pertussis to high levels in multi-copy transformants of the industrial yeast Pichia pastoris. In high-density fermentations, engineered P. pastoris yielded greater than 3 g of the protein per litre of culture. Purified recombinant pertactin was able to stimulate the incomplete protection afforded by toxoid to the level of the whole-cell vaccine, as shown by the Kendrick test, supporting its inclusion in future acellular vaccines...
December 1991: Vaccine
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