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Pertussis culture

Lucille L F van Beek, Daan D de Gouw, Marc M J Eleveld, Hester H J Bootsma, Marien M I de Jonge, Frits F R Mooi, Aldert A Zomer, Dimitri D A Diavatopoulos
There is a lack of insight into the basic mechanisms by which Bordetella pertussis adapts to the local host environment during infection. We analysed B. pertussis gene expression in the upper and lower airways of mice and compared this to SO4-induced in vitro Bvg-regulated gene transcription. Approximately 30% of all genes were found to be differentially expressed between in vitro vs. in vivo conditions. This included several novel potential vaccine antigens that were exclusively expressed in vivo. Significant differences in expression profile and metabolic pathways were identified between the upper versus the lower airways, suggesting distinct antigenic profiles...
March 8, 2018: Journal of Infectious Diseases
Nathan Saul, Kevin Wang, Shopna Bag, Heather Baldwin, Kate Alexander, Meena Chandra, Jane Thomas, Helen Quinn, Vicky Sheppeard, Stephen Conaty
BACKGROUND: Infants are at the highest risk of severe complications - including death - as a result of pertussis infection. Controlling pertussis in this group has been challenging, particularly in those too young to be vaccinated. Following revised national recommendations in March 2015, the state of New South Wales, Australia, introduced a funded maternal vaccination campaign at 28 - 32 weeks of gestation using a 3-component tetanus-diphtheria-acellular pertussis vaccine (dTpa; Boostrix, GSK)...
February 28, 2018: Vaccine
Sushena Krishnaswamy, Allen C Cheng, Euan M Wallace, Jim Buttery, Michelle Giles
The role of maternal vaccination in reducing neonatal morbidity and mortality is expanding but uptake remains suboptimal. While the barriers to uptake have been well described, women from minority groups have not been well represented in previous studies. In this study we examine the facilitators and barriers to uptake of antenatal vaccination by women from culturally and linguistically diverse backgrounds in Melbourne, Australia. 537 women attending antenatal care completed a survey; 69% were born overseas...
March 1, 2018: Human Vaccines & Immunotherapeutics
Yefei Pang, Peter Thomas
Progesterone effects on vascular smooth muscle cell (VSMC) relaxation and the mechanism were investigated in cultured human umbilical vein VSMCs. Membrane progesterone receptors mPRα, mPRβ, and mPRγ were highly expressed in VSMCs, whereas nuclear progesterone receptor (nPR) had low expression. Progesterone (20 nM) and 02-0 (mPR-selective agonist), but not R5020 (nPR agonist), induced muscle relaxation in both a VSMC collagen gel disk contraction assay and an endothelium-denuded human umbilical artery ring tension assay...
February 8, 2018: Molecular and Cellular Endocrinology
NMdu Plessis, G Ntshoe, G Reubenson, R Kularatne, L Blumberg, J Thomas, T Avenant
BACKGROUND: In low and middle-income countries, including South Africa, the epidemiology of pertussis in relation to immunisation, nutritional and HIV status is poorly described. We report on risk factors in South African children hospitalised with pertussis. METHODS: We conducted a prospective, hospital-based, sentinel surveillance programme for pertussis in Gauteng Province, South Africa. Hospitalised children (≤10 years) meeting the surveillance criteria for clinically suspected pertussis were screened and enrolled...
February 1, 2018: International Journal of Infectious Diseases: IJID
Karoline Wagner, Burkard Springer, Frank Imkamp, Onya Opota, Gilbert Greub, Peter M Keller
Pneumonia is a severe infectious disease. In addition to common viruses and bacterial pathogens (e.g. Streptococcus pneumoniae), fastidious respiratory pathogens like Chlamydia pneumoniae, Mycoplasma pneumoniae and Legionella spp. can cause severe atypical pneumonia. They do not respond to penicillin derivatives, which may cause failure of antibiotic empirical therapy. The same applies for infections with B. pertussis and B. parapertussis, the cause of pertussis disease, that may present atypically and need to be treated with macrolides...
January 31, 2018: International Journal of Medical Microbiology: IJMM
Bert Baumgaertner, Juliet E Carlisle, Florian Justwan
In light of the increasing refusal of some parents to vaccinate children, public health strategies have focused on increasing knowledge and awareness based on a "knowledge-deficit" approach. However, decisions about vaccination are based on more than mere knowledge of risks, costs, and benefits. Individual decision making about vaccinating involves many other factors including those related to emotion, culture, religion, and socio-political context. In this paper, we use a nationally representative internet survey in the U...
2018: PloS One
Yumi Matsushita, Miki Manabe, Naoki Kitamura, Izumi Shibuya
Transient receptor potential vanilloid type 1 (TRPV1) is a polymodal receptor channel that responds to multiple types of stimuli, such as heat, acid, mechanical pressure and some vanilloids. Capsaicin is the most commonly used vanilloid to stimulate TRPV1. TRPV1 channels are expressed in dorsal root ganglion neurons that extend to Aδ- and C-fibers and have a role in the transduction of noxious inputs to the skin into the electrical signals of the sensory nerve. Although noradrenergic nervous systems, including the descending antinociceptive system and the sympathetic nervous system, are known to modulate pain sensation, the functional association between TRPV1 and noradrenaline in primary sensory neurons has rarely been examined...
2018: PloS One
Ayano Usuba, Yuko Komase, Baku Oyama, Takahiro Tsuburai, Naoya Hida
Bordetella pertussis isolation by culture has low detection sensitivity for diagnosing pertussis; the diagnosis is confirmed by measuring serum anti-pertussis toxin (anti-PT) or anti-filamentous hemagglutinin antibody titers, and by genetic testing (polymerase chain reaction/loop-mediated isothermal amplification). Isolation of B. pertussis in adults is difficult, resulting in a delayed diagnosis, as a delayed cough may present ≥3 months after onset. Differentiation from bronchial asthma is also important...
2017: Arerugī, [Allergy]
Masoumeh Nakhost Lotfi, Vajiheh Sadat Nikbin, Omid Nasiri, Farzad Badmasti, Fereshteh Shahcheraghi
Background and Objectives: Bordetella holmesii is associated with a pertussis-like respiratory syndrome in healthy individuals and also a rare cause of septicaemia, endocarditis, pneumonia, and septic arthritis, mostly in immunocompromised patients. Culture technique and real-time PCR are 2 methods used to detect Bordetella spp. Materials and Methods: In this study, 435 nasopharyngeal specimens of patients with suspected whooping cough were checked for the presence of B...
August 2017: Iranian Journal of Microbiology
Shakir Hasan, Nikhil Nitin Kulkarni, Arni Asbjarnarson, Irena Linhartova, Radim Osicka, Peter Sebo, Gudmundur H Gudmundsson
The airway epithelium restricts the penetration of inhaled pathogens into the underlying tissue and plays a crucial role in the innate immune defense against respiratory infections. The whooping cough agent, Bordetella pertussis , adheres to ciliated cells of the human airway epithelium and subverts its defense functions through the action of secreted toxins and other virulence factors. We examined the impact of B. pertussis infection and of adenylate cyclase toxin-hemolysin (CyaA) action on the functional integrity of human bronchial epithelial cells cultured at the air-liquid interface (ALI)...
March 2018: Infection and Immunity
Terra Manca
In recent years, the Canadian province of Alberta experienced outbreaks of measles, mumps, pertussis, and influenza. Even so, the dominant cultural narrative maintains that vaccines are safe, effective, and necessary to maintain population health. Many vaccine supporters have expressed anxieties that stories contradicting this narrative have lowered herd immunity levels because they frighten the public into avoiding vaccination. As such, vaccine policies often emphasize educating parents and the public about the importance and safety of vaccination...
January 2018: Social Science & Medicine
D Bottero, M E Zurita, M E Gaillard, E Bartel, C Vercellini, D Hozbor
Bordetella bronchiseptica , a Gram-negative bacterium, causes chronic respiratory tract infections in a wide variety of mammalian hosts, including humans (albeit rarely). We recently designed Bordetella pertussis and Bordetella parapertussis experimental vaccines based on outer membrane vesicles (OMVs) derived from each pathogen, and we obtained protection against the respective infections in mice. Here, we demonstrated that OMVs derived from virulent-phase B. bronchiseptica (OMVBbvir+ ) protected mice against sublethal infections with different B...
February 15, 2018: Applied and Environmental Microbiology
Piia Jõgi, Marje Oona, Tanel Kaart, Karolin Toompere, Tereza Maskina, Iris Koort, Anneli Rätsep, Irja Lutsar
PURPOSE: We aimed to determine the prevalence, symptoms and course of pertussis and parapertussis among patients at any age with a cough of unknown aetiology that had lasted for ≥ 7 days and to assess the diagnostic value of the symptoms included in the World Health Organisations' (WHO) clinical case definition of pertussis. METHODS: Patients were enrolled between the 23 April 2012 and 31 December 2014 at 25 general practitioner (GP) centres and three paediatric hospitals...
November 1, 2017: Infection
Yung-Yi C Mosley, Fangjia Lu, Harm HogenEsch
Cell-mediated immune responses characterized by the secretion of IFNγ and IL-17 play an important role in the immune response to Bordetella pertussis (B. pertussis). We investigated innate sources of IFNγ and IL-17 upon stimulation of spleen cells from BALB/c (B/c) and C57BL/6 (B6) mice with heat-killed B. pertussis (hkBp). Spleen cells from B/c mice secreted less IFNγ and more IL-17 than those from B6 mice. Innate IFNγ was produced predominantly by NK cells in B/c mice and by CD8 T cells and NK cells in B6 mice...
December 2017: Immunologic Research
Nicole Guiso, Corinne Levy, Olivier Romain, Sophie Guillot, Andreas Werner, Marie Charlotte Rondeau, Stéphane Béchet, Robert Cohen
BACKGROUND: Increasing incidence of whooping cough (pertussis) has been reported in many countries, attributed to a switch from whole-cell pertussis-containing vaccine (wPV) to acellular PV (aPV) and circulation of the pertactin non-producing Bordetella pertussis. The present study aimed to estimate the duration of immunity conferred by PVs in children in France with data from an ongoing pediatric ambulatory surveillance of pertussis. METHODS: A total of 64 pediatricians throughout France enrolled children with suspected pertussis...
October 27, 2017: Vaccine
Li Wei, Kyohei Tokizane, Hiroyuki Konishi, Hua-Rong Yu, Hiroshi Kiyama
BACKGROUND: Several G-protein-coupled receptors (GPCRs) have been shown to be important signaling mediators between neurons and glia. In our previous screening for identification of nerve injury-associated GPCRs, G-protein-coupled receptor 84 (GPR84) mRNA showed the highest up-regulation by microglia after nerve injury. GPR84 is a pro-inflammatory receptor of macrophages in a neuropathic pain mouse model, yet its function in resident microglia in the central nervous system is poorly understood...
October 3, 2017: Journal of Neuroinflammation
Kazue Hisaoka-Nakashima, Chie Matsumoto, Honami Azuma, Sayaka Taki, Minoru Takebayashi, Yoshihiro Nakata, Norimitsu Morioka
A significant reduction of glial cell line-derived neurotrophic factor (GDNF) has been identified in the pathophysiology of neurodegenerative and neuropsychiatric disorders. Thus, clarification of the mechanism of GDNF production, and modulating brain GDNF levels could be a novel therapeutic approach. A previous study demonstrated that antidepressant amitriptyline-induced GDNF production was significantly inhibited by pertussis toxin (PTX), a Gi/o protein inhibitor in astrocytes, the main source of GDNF in the brain...
2017: Biological & Pharmaceutical Bulletin
Christine C Krieger, Joseph D Perry, Sarah J Morgan, George J Kahaly, Marvin C Gershengorn
We previously showed that thyrotropin (TSH)/insulinlike growth factor (IGF)-1 receptor cross-talk appears to be involved in Graves' orbitopathy (GO) pathogenesis and upregulation of thyroid-specific genes in human thyrocytes. In orbital fibroblasts from GO patients, coadministration of TSH and IGF-1 induces synergistic increases in hyaluronan secretion. In human thyrocytes, TSH plus IGF-1 synergistically increased expression of the sodium-iodide symporter that appeared to involve ERK1/2 activation. However, the details of ERK1/2 activation were not known, nor was whether ERK1/2 was involved in this synergism in other cell types...
October 1, 2017: Endocrinology
Keita Odanaka, Masato Iwatsuki, Tomomitsu Satho, Mineo Watanabe
Culture supernatants of Bordetella pertussis are a brilliant yellow; however, the structure and biological role of the responsible pigment have not been investigated. In this study, a brilliant yellow-colored fraction was extracted from culture supernatants of B. pertussis and analyzed by HPLC. UV-visible spectral analysis and mass spectrometry identified the brilliant yellow pigment as riboflavin. Riboflavin production was high in lag and early log phases and riboflavin was found to enhance growth of B. pertussis in low-density cultures...
September 14, 2017: Microbiology and Immunology
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