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Pertussis culture

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https://www.readbyqxmd.com/read/29094317/pertussis-and-parapertussis-in-children-and-adults-with-a-persistent-cough-an-observational-study
#1
Piia Jõgi, Marje Oona, Tanel Kaart, Karolin Toompere, Tereza Maskina, Iris Koort, Anneli Rätsep, Irja Lutsar
PURPOSE: We aimed to determine the prevalence, symptoms and course of pertussis and parapertussis among patients at any age with a cough of unknown aetiology that had lasted for ≥ 7 days and to assess the diagnostic value of the symptoms included in the World Health Organisations' (WHO) clinical case definition of pertussis. METHODS: Patients were enrolled between the 23 April 2012 and 31 December 2014 at 25 general practitioner (GP) centres and three paediatric hospitals...
November 1, 2017: Infection
https://www.readbyqxmd.com/read/29052125/differences-in-innate-ifn%C3%AE-and-il-17-responses-to-bordetella-pertussis-between-balb-c-and-c57bl-6-mice-role-of-%C3%AE-%C3%AE-t-cells-nk-cells-and-dendritic-cells
#2
Yung-Yi C Mosley, Fangjia Lu, Harm HogenEsch
Cell-mediated immune responses characterized by the secretion of IFNγ and IL-17 play an important role in the immune response to Bordetella pertussis (B. pertussis). We investigated innate sources of IFNγ and IL-17 upon stimulation of spleen cells from BALB/c (B/c) and C57BL/6 (B6) mice with heat-killed B. pertussis (hkBp). Spleen cells from B/c mice secreted less IFNγ and more IL-17 than those from B6 mice. Innate IFNγ was produced predominantly by NK cells in B/c mice and by CD8 T cells and NK cells in B6 mice...
October 19, 2017: Immunologic Research
https://www.readbyqxmd.com/read/28974408/whooping-cough-surveillance-in-france-in-pediatric-private-practice-in-2006-2015
#3
Nicole Guiso, Corinne Levy, Olivier Romain, Sophie Guillot, Andreas Werner, Marie Charlotte Rondeau, Stéphane Béchet, Robert Cohen
BACKGROUND: Increasing incidence of whooping cough (pertussis) has been reported in many countries, attributed to a switch from whole-cell pertussis-containing vaccine (wPV) to acellular PV (aPV) and circulation of the pertactin non-producing Bordetella pertussis. The present study aimed to estimate the duration of immunity conferred by PVs in children in France with data from an ongoing pediatric ambulatory surveillance of pertussis. METHODS: A total of 64 pediatricians throughout France enrolled children with suspected pertussis...
October 27, 2017: Vaccine
https://www.readbyqxmd.com/read/28974234/agonists-for-g-protein-coupled-receptor-84-gpr84-alter-cellular-morphology-and-motility-but-do-not-induce-pro-inflammatory-responses-in-microglia
#4
Li Wei, Kyohei Tokizane, Hiroyuki Konishi, Hua-Rong Yu, Hiroshi Kiyama
BACKGROUND: Several G-protein-coupled receptors (GPCRs) have been shown to be important signaling mediators between neurons and glia. In our previous screening for identification of nerve injury-associated GPCRs, G-protein-coupled receptor 84 (GPR84) mRNA showed the highest up-regulation by microglia after nerve injury. GPR84 is a pro-inflammatory receptor of macrophages in a neuropathic pain mouse model, yet its function in resident microglia in the central nervous system is poorly understood...
October 3, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28966248/pharmacological-activation-gi-o-protein-increases-glial-cell-line-derived-neurotrophic-factor-production-through-fibroblast-growth-factor-receptor-and-extracellular-signal-regulated-kinase-pathway-in-primary-cultured-rat-cortical-astrocytes
#5
Kazue Hisaoka-Nakashima, Chie Matsumoto, Honami Azuma, Sayaka Taki, Minoru Takebayashi, Yoshihiro Nakata, Norimitsu Morioka
A significant reduction of glial cell line-derived neurotrophic factor (GDNF) has been identified in the pathophysiology of neurodegenerative and neuropsychiatric disorders. Thus, clarification of the mechanism of GDNF production, and modulating brain GDNF levels could be a novel therapeutic approach. A previous study demonstrated that antidepressant amitriptyline-induced GDNF production was significantly inhibited by pertussis toxin (PTX), a Gi/o protein inhibitor in astrocytes, the main source of GDNF in the brain...
2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28938449/tsh-igf-1-receptor-cross-talk-rapidly-activates-extracellular-signal-regulated-kinases-in-multiple-cell-types
#6
Christine C Krieger, Joseph D Perry, Sarah J Morgan, George J Kahaly, Marvin C Gershengorn
We previously showed that thyrotropin (TSH)/insulinlike growth factor (IGF)-1 receptor cross-talk appears to be involved in Graves' orbitopathy (GO) pathogenesis and upregulation of thyroid-specific genes in human thyrocytes. In orbital fibroblasts from GO patients, coadministration of TSH and IGF-1 induces synergistic increases in hyaluronan secretion. In human thyrocytes, TSH plus IGF-1 synergistically increased expression of the sodium-iodide symporter that appeared to involve ERK1/2 activation. However, the details of ERK1/2 activation were not known, nor was whether ERK1/2 was involved in this synergism in other cell types...
October 1, 2017: Endocrinology
https://www.readbyqxmd.com/read/28906022/identification-and-characterization-of-a-brilliant-yellow-pigment-produced-by-bordetella-pertussis
#7
Keita Odanaka, Masato Iwatsuki, Tomomitsu Satho, Mineo Watanabe
Culture supernatants of Bordetella pertussis are a brilliant yellow; however, the structure and biological role of the responsible pigment have not been investigated. In this study, a brilliant yellow-colored fraction was extracted from culture supernatants of B. pertussis and analyzed by HPLC. UV-visible spectral analysis and mass spectrometry identified the brilliant yellow pigment as riboflavin. Riboflavin production was high in lag and early log phases and riboflavin was found to enhance growth of B. pertussis in low-density cultures...
September 14, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28867343/assessment-of-clinical-symptoms-in-household-contacts-of-confirmed-pertussis-cases
#8
Angela Domínguez, Núria Soldevila, Joan A Caylà, Manuel García-Cenoz, Glòria Ferrús, Maria Rosa Sala-Farré, Josep Álvarez, Mònica Carol, Irene Barrabeig, Neus Camps, Lorena Coronas, Carmen Muñoz-Almagro, Pere Godoy
OBJECTIVES: We assessed the value of the clinical symptoms included in the case definition of pertussis in household contacts of laboratory-confirmed cases. METHODS: A prospective epidemiological study was made in two Spanish regions. Household contacts were identified for each confirmed case reported during 2012 and 2013. Two clinical samples were taken to determine the presence or absence of Bordetella pertussis by culture or real-time PCR. Clinical variables, age and vaccination status were recorded...
September 1, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28715411/a-curated-genome-scale-metabolic-model-of-bordetella-pertussis-metabolism
#9
Nick Fyson, Jerry King, Thomas Belcher, Andrew Preston, Caroline Colijn
The Gram-negative bacterium Bordetella pertussis is the causative agent of whooping cough, a serious respiratory infection causing hundreds of thousands of deaths annually worldwide. There are effective vaccines, but their production requires growing large quantities of B. pertussis. Unfortunately, B. pertussis has relatively slow growth in culture, with low biomass yields and variable growth characteristics. B. pertussis also requires a relatively expensive growth medium. We present a new, curated flux balance analysis-based model of B...
July 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28622957/real-time-polymerase-chain-reaction-based-detection-of-bordetella-pertussis-in-mexican-infants-and-their-contacts-a-3-year-multicenter-study
#10
MULTICENTER STUDY
Alejandra Aquino-Andrade, Gabriel Martínez-Leyva, Jocelin Mérida-Vieyra, Patricia Saltigeral, Antonino Lara, Wendy Domínguez, Silvestre García de la Puente, Agustín De Colsa
OBJECTIVES: To evaluate the usefulness of real-time polymerase chain reaction (RT-PCR) as a diagnostic method for the detection of Bordetella pertussis in hospitalized patients aged <1 year with a clinical diagnosis of whooping cough, as well as to identify the role of household contacts as a source of infection. STUDY DESIGN: This was a prospective, multicenter study of infants aged <1 year who were hospitalized with symptoms suggestive of whooping cough...
September 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/28538850/-infection-by-bordetella-pertussis-and-bordetella-parapertussis-in-cases-of-suspected-whooping-cough-2011-2015-in-mar-del-plata-argentina
#11
Silvina Lavayén, Claudia Zotta, Marcela Cepeda, Claudia Lara, Analia Rearte, Mabel Regueira
Objectives: . To classify the study population in the Argentinian National Health Surveillance System framework, determine the proportion of infection by Bordetella pertussis and Bordetella parapertussis, and identify factors associated with the cases of suspected whooping cough attended to in the city of Mar del Plata and its outskirts during the period 2011- 2015. Materials and Methods: An observational and descriptive study was carried out. Clinical cases with suspicion of whooping cough were diagnosed by laboratory...
January 2017: Revista Peruana de Medicina Experimental y Salud Pública
https://www.readbyqxmd.com/read/28511667/epidemiology-of-pertussis-in-casablanca-morocco-contribution-of-conventional-and-molecular-diagnosis-tools
#12
Khalid Katfy, Nicole Guiso, Idrissa Diawara, Khalid Zerouali, Bouchra Slaoui, Zineb Jouhadi, Abdelhadi Zineddine, Houria Belabbes, Naima Elmdaghri
BACKGROUND: Pertussis, a vaccine preventable disease, is still responsible of significant morbidity and mortality around the world, mostly in newborns. The aim of the present study was (1) to introduce pertussis surveillance in the major pediatric hospital of Casablanca (2) to analyze the prevalence of pertussis among children under 14 years of age and their entourage in Casablanca, Morocco. METHODS: This is a prospective and non-case controlled study, including children suspected of Pertussis admitted at the Abderrahim Harouchi Pediatric Hospital in Casablanca, from January 2013 to June 2015...
May 16, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28277139/sphingosine-1-phosphate-activates-the-akt-pathway-to-inhibit-chemotherapy-induced-human-granulosa-cell-apoptosis
#13
Shuyi Li, Jianling Chen, Xiaoling Fang, Xiaomeng Xia
To investigate whether sphingosine-1-phosphate (S1P), an apoptosis-inhibitor would be able to inhibit chemotherapy induced human granulosa cell apoptosis. Cultures of primary granulosa cells were isolated from women undergoing in vitro fertilization (IVF). MTT assay was used to measure the optimum concentration of CTX and S1P acts on human granulosa cells. Granulosa cells were added with pertussis toxin (PTX), the PI3K inhibitor LY294002. Western blot analysis was used to analyze the signaling pathway of proteins and cell apoptosis...
February 21, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28242451/characterisation-of-the-bordetella-pertussis-secretome-under-different-media
#14
Laurence Don Wai Luu, Sophie Octavia, Ling Zhong, Mark Raftery, Vitali Sintchenko, Ruiting Lan
Our understanding of the Bordetella pertussis secretome remains limited including the role of different growth conditions in the secretome. In this study the secretome of L1423, a clinical isolate from the 2008-2012 Australian epidemic, cultured on Stainer-Scholte (SS) and Thalen-IJssel (THIJS) media for 12h was characterised using liquid chromatography-mass spectrometry (LC-MS/MS). In the supernatant, LC-MS/MS identified 260 proteins with 143 bioinformatically predicted to be secreted. Eighty percent of proteins were identified in both media...
March 31, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28197070/role-of-app-interactions-with-heterotrimeric-g-proteins-physiological-functions-and-pathological-consequences
#15
REVIEW
Philip F Copenhaver, Donat Kögel
Following the discovery that the amyloid precursor protein (APP) is the source of β-amyloid peptides (Aβ) that accumulate in Alzheimer's disease (AD), structural analyses suggested that the holoprotein resembles a transmembrane receptor. Initial studies using reconstituted membranes demonstrated that APP can directly interact with the heterotrimeric G protein Gαo (but not other G proteins) via an evolutionarily G protein-binding motif in its cytoplasmic domain. Subsequent investigations in cell culture showed that antibodies against the extracellular domain of APP could stimulate Gαo activity, presumably mimicking endogenous APP ligands...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28129036/severe-pertussis-infection-in-infants-less-than-6-months-of-age-clinical-manifestations-and-molecular-characterization
#16
Paola Stefanelli, Gabriele Buttinelli, Paola Vacca, Alberto E Tozzi, Fabio Midulla, Rita Carsetti, Giorgio Fedele, Alberto Villani, Carlo Concato
We conducted a study to determine the main traits of pertussis among unimmunized infants less than 6 months of age. From August 2012 to March 2015, 141 nasopharyngeal aspirates (NPAs) were collected from infants with respiratory symptoms attending 2 major hospitals in Rome. Clinical data were recorded and analyzed. Lab-confirmation was performed by culture and realtime PCR. B. pertussis virulence-associated genes (ptxP, ptxA and prn), together with multilocus variable-number tandem repeat analysis (MLVA), were also investigated by the sequence-based analysis on the DNAs extracted from positive samples...
May 4, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28039663/whooping-cough-in-adults-a-series-of-severe-cases
#17
K Zycinska, M Cieplak, M Chmielewska, A Nitsch-Osuch, A Klaczkow, M Hadzik-Blaszczyk, Z Kur, K A Wardyn
Bordetella pertussis is a gram-negative aerobic coccobacillus causing contagious respiratory tract disease called whooping cough. The virulence factors consist of pertussis toxin, filamentous hemagglutinin, fimbriae, lipooligosaccharide, and adenylate cyclase toxin. The disease causes a worldwide threat to public health despite a high vaccination coverage. The course of whooping cough in adults is frequently atypical, causing difficulty in diagnosis. In this report we present five patients hospitalized with Bordetella pertussis infection manifesting atypical and severe symptoms...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28008331/a-meningococcal-outer-membrane-vesicle-vaccine-incorporating-genetically-attenuated-endotoxin-dissociates-inflammation-from-immunogenicity
#18
David J Dowling, Holly Sanders, Wing Ki Cheng, Sweta Joshi, Spencer Brightman, Ilana Bergelson, Carlo Pietrasanta, Simon D van Haren, Sandra van Amsterdam, Jeffrey Fernandez, Germie P J M van den Dobbelsteen, Ofer Levy
BACKGROUND: Group B Neisseria meningitidis, an endotoxin-producing Gram-negative bacterium, causes the highest incidence of group B meningococcus (MenB) disease in the first year of life. The Bexsero vaccine is indicated in Europe from 8 weeks of age. Endotoxin components of outer membrane vesicles (OMVs) or soluble lipopolysaccharide (LPS) represent a potential source of inflammation and residual reactogenicity. The purpose of this study was to compare novel candidate MenB vaccine formulations with licensed vaccines, including Bexsero, using age-specific human in vitro culture systems...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27977922/proteome-analysis-is-a-valuable-tool-to-monitor-antigen-expression-during-upstream-processing-of-whole-cell-pertussis-vaccines
#19
Bernard Metz, Marieke Hoonakker, Joost P Uittenbogaard, Michel Weyts, Geert P M Mommen, Hugo D Meiring, Wichard Tilstra, Jeroen L A Pennings, Leo A van der Pol, Betsy Kuipers, Arjen Sloots, Jan van den IJssel, Bas van de Waterbeemd, Arno van der Ark
Physicochemical and immunochemical assays were applied to substantiate the relation between upstream processing and the quality of whole-cell pertussis vaccines. Bordetella pertussis bacteria were cultured on a chemically defined medium using a continuous cultivation process in stirred tank reactors to obtain uniform protein expression. Continuous culture favors the consistent production of proteins known as virulence factors. Magnesium sulfate was added during the steady state of the culture in order to diminish the expression of virulence proteins...
February 3, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/27886031/pertussis-in-north-central-and-northwestern-regions-of-algeria
#20
Nabila Benamrouche, Hassiba Tali Maamar, Malika Lazri, Sonia Hasnaoui, Abdelkarim Radoui, Ourida Lafer, Rachida Boukari, Chawki Kaddache, Zakia Arrada, Kheira Rahal
INTRODUCTION: Pertussis outbreaks continue to occur in many countries despite high vaccination coverage. Under-diagnosed cases in adolescents and adults may result in increased transmission to infants, who are at risk of severe pertussis. Additional measures to protect both groups should be considered. METHODOLOGY: Nasopharyngeal samples and sera were collected from patients and household contacts with clinically suspected pertussis. Diagnoses were confirmed by culture, real-time polymerase chain reaction (PCR), and serology...
November 24, 2016: Journal of Infection in Developing Countries
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