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Pertussis culture

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https://www.readbyqxmd.com/read/29304162/adrenergic-receptors-inhibit-trpv1-activity-in-the-dorsal-root-ganglion-neurons-of-rats
#1
Yumi Matsushita, Miki Manabe, Naoki Kitamura, Izumi Shibuya
Transient receptor potential vanilloid type 1 (TRPV1) is a polymodal receptor channel that responds to multiple types of stimuli, such as heat, acid, mechanical pressure and some vanilloids. Capsaicin is the most commonly used vanilloid to stimulate TRPV1. TRPV1 channels are expressed in dorsal root ganglion neurons that extend to Aδ- and C-fibers and have a role in the transduction of noxious inputs to the skin into the electrical signals of the sensory nerve. Although noradrenergic nervous systems, including the descending antinociceptive system and the sympathetic nervous system, are known to modulate pain sensation, the functional association between TRPV1 and noradrenaline in primary sensory neurons has rarely been examined...
2018: PloS One
https://www.readbyqxmd.com/read/29249756/-a-case-of-pertussis-in-adult-that-could-be-diagnosed-by-detection-of-bordetella-pertussis-from-sputum
#2
Ayano Usuba, Yuko Komase, Baku Oyama, Takahiro Tsuburai, Naoya Hida
Bordetella pertussis isolation by culture has low detection sensitivity for diagnosing pertussis; the diagnosis is confirmed by measuring serum anti-pertussis toxin (anti-PT) or anti-filamentous hemagglutinin antibody titers, and by genetic testing (polymerase chain reaction/loop-mediated isothermal amplification). Isolation of B. pertussis in adults is difficult, resulting in a delayed diagnosis, as a delayed cough may present ≥3 months after onset. Differentiation from bronchial asthma is also important...
2017: Arerugī, [Allergy]
https://www.readbyqxmd.com/read/29238457/molecular-detection-of-bordetella-holmesii-in-two-infants-with-pertussis-like-syndrome-the-first-report-from-iran
#3
Masoumeh Nakhost Lotfi, Vajiheh Sadat Nikbin, Omid Nasiri, Farzad Badmasti, Fereshteh Shahcheraghi
Background and Objectives: Bordetella holmesii is associated with a pertussis-like respiratory syndrome in healthy individuals and also a rare cause of septicaemia, endocarditis, pneumonia, and septic arthritis, mostly in immunocompromised patients. Culture technique and real-time PCR are 2 methods used to detect Bordetella spp. Materials and Methods: In this study, 435 nasopharyngeal specimens of patients with suspected whooping cough were checked for the presence of B...
August 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29203545/bordetella-pertussis-adenylate-cyclase-toxin-disrupts-functional-integrity-of-bronchial-epithelial-layers
#4
Shakir Hasan, Nikhil Nitin Kulkarni, Arni Asbjarnarson, Irena Linhartova, Radim Osicka, Peter Sebo, Gudmundur H Gudmundsson
Airway epithelium restricts penetration of inhaled pathogens into the underlying tissue and plays a crucial role in innate immune defense against respiratory infections. The whooping cough agent, Bordetella pertussis, adheres to ciliated cells of human airway epithelium and subverts its defense functions through the action of secreted toxins and other virulence factors. We have examined the impact of B. pertussis infection and of adenylate cyclase toxin (CyaA) action on the functional integrity of air-liquid interface (ALI)-cultured human bronchial epithelial cells...
December 4, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/29195189/-one-of-the-greatest-medical-success-stories-physicians-and-nurses-small-stories-about-vaccine-knowledge-and-anxieties
#5
Terra Manca
In recent years, the Canadian province of Alberta experienced outbreaks of measles, mumps, pertussis, and influenza. Even so, the dominant cultural narrative maintains that vaccines are safe, effective, and necessary to maintain population health. Many vaccine supporters have expressed anxieties that stories contradicting this narrative have lowered herd immunity levels because they frighten the public into avoiding vaccination. As such, vaccine policies often emphasize educating parents and the public about the importance and safety of vaccination...
November 20, 2017: Social Science & Medicine
https://www.readbyqxmd.com/read/29180369/membrane-vesicles-derived-from-bordetella-bronchiseptica-active-constituent-of-a-new-vaccine-against-infections-caused-by-this-pathogen
#6
D Bottero, M E Zurita, M E Gaillard, E Bartel, C Vercellini, D Hozbor
Bordetella bronchiseptica, a Gram-negative bacterium, causes chronic respiratory-tract infections in a wide variety of mammalian hosts-humans included, albeit rarely. We recently designed B. pertussis and B. parapertussis experimental vaccines based on outer-membrane vesicles derived from each pathogen and obtained protection against the respective infections in mice. Here, we demonstrate that outer-membrane vesicles derived from virulent-phase B. bronchiseptica (OMVBbvir+) protect mice against sublethal infection from different B...
November 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29094317/pertussis-and-parapertussis-in-children-and-adults-with-a-persistent-cough-an-observational-study
#7
Piia Jõgi, Marje Oona, Tanel Kaart, Karolin Toompere, Tereza Maskina, Iris Koort, Anneli Rätsep, Irja Lutsar
PURPOSE: We aimed to determine the prevalence, symptoms and course of pertussis and parapertussis among patients at any age with a cough of unknown aetiology that had lasted for ≥ 7 days and to assess the diagnostic value of the symptoms included in the World Health Organisations' (WHO) clinical case definition of pertussis. METHODS: Patients were enrolled between the 23 April 2012 and 31 December 2014 at 25 general practitioner (GP) centres and three paediatric hospitals...
November 1, 2017: Infection
https://www.readbyqxmd.com/read/29052125/differences-in-innate-ifn%C3%AE-and-il-17-responses-to-bordetella-pertussis-between-balb-c-and-c57bl-6-mice-role-of-%C3%AE-%C3%AE-t-cells-nk-cells-and-dendritic-cells
#8
Yung-Yi C Mosley, Fangjia Lu, Harm HogenEsch
Cell-mediated immune responses characterized by the secretion of IFNγ and IL-17 play an important role in the immune response to Bordetella pertussis (B. pertussis). We investigated innate sources of IFNγ and IL-17 upon stimulation of spleen cells from BALB/c (B/c) and C57BL/6 (B6) mice with heat-killed B. pertussis (hkBp). Spleen cells from B/c mice secreted less IFNγ and more IL-17 than those from B6 mice. Innate IFNγ was produced predominantly by NK cells in B/c mice and by CD8 T cells and NK cells in B6 mice...
October 19, 2017: Immunologic Research
https://www.readbyqxmd.com/read/28974408/whooping-cough-surveillance-in-france-in-pediatric-private-practice-in-2006-2015
#9
Nicole Guiso, Corinne Levy, Olivier Romain, Sophie Guillot, Andreas Werner, Marie Charlotte Rondeau, Stéphane Béchet, Robert Cohen
BACKGROUND: Increasing incidence of whooping cough (pertussis) has been reported in many countries, attributed to a switch from whole-cell pertussis-containing vaccine (wPV) to acellular PV (aPV) and circulation of the pertactin non-producing Bordetella pertussis. The present study aimed to estimate the duration of immunity conferred by PVs in children in France with data from an ongoing pediatric ambulatory surveillance of pertussis. METHODS: A total of 64 pediatricians throughout France enrolled children with suspected pertussis...
October 27, 2017: Vaccine
https://www.readbyqxmd.com/read/28974234/agonists-for-g-protein-coupled-receptor-84-gpr84-alter-cellular-morphology-and-motility-but-do-not-induce-pro-inflammatory-responses-in-microglia
#10
Li Wei, Kyohei Tokizane, Hiroyuki Konishi, Hua-Rong Yu, Hiroshi Kiyama
BACKGROUND: Several G-protein-coupled receptors (GPCRs) have been shown to be important signaling mediators between neurons and glia. In our previous screening for identification of nerve injury-associated GPCRs, G-protein-coupled receptor 84 (GPR84) mRNA showed the highest up-regulation by microglia after nerve injury. GPR84 is a pro-inflammatory receptor of macrophages in a neuropathic pain mouse model, yet its function in resident microglia in the central nervous system is poorly understood...
October 3, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28966248/pharmacological-activation-gi-o-protein-increases-glial-cell-line-derived-neurotrophic-factor-production-through-fibroblast-growth-factor-receptor-and-extracellular-signal-regulated-kinase-pathway-in-primary-cultured-rat-cortical-astrocytes
#11
Kazue Hisaoka-Nakashima, Chie Matsumoto, Honami Azuma, Sayaka Taki, Minoru Takebayashi, Yoshihiro Nakata, Norimitsu Morioka
A significant reduction of glial cell line-derived neurotrophic factor (GDNF) has been identified in the pathophysiology of neurodegenerative and neuropsychiatric disorders. Thus, clarification of the mechanism of GDNF production, and modulating brain GDNF levels could be a novel therapeutic approach. A previous study demonstrated that antidepressant amitriptyline-induced GDNF production was significantly inhibited by pertussis toxin (PTX), a Gi/o protein inhibitor in astrocytes, the main source of GDNF in the brain...
2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28938449/tsh-igf-1-receptor-cross-talk-rapidly-activates-extracellular-signal-regulated-kinases-in-multiple-cell-types
#12
Christine C Krieger, Joseph D Perry, Sarah J Morgan, George J Kahaly, Marvin C Gershengorn
We previously showed that thyrotropin (TSH)/insulinlike growth factor (IGF)-1 receptor cross-talk appears to be involved in Graves' orbitopathy (GO) pathogenesis and upregulation of thyroid-specific genes in human thyrocytes. In orbital fibroblasts from GO patients, coadministration of TSH and IGF-1 induces synergistic increases in hyaluronan secretion. In human thyrocytes, TSH plus IGF-1 synergistically increased expression of the sodium-iodide symporter that appeared to involve ERK1/2 activation. However, the details of ERK1/2 activation were not known, nor was whether ERK1/2 was involved in this synergism in other cell types...
October 1, 2017: Endocrinology
https://www.readbyqxmd.com/read/28906022/identification-and-characterization-of-a-brilliant-yellow-pigment-produced-by-bordetella-pertussis
#13
Keita Odanaka, Masato Iwatsuki, Tomomitsu Satho, Mineo Watanabe
Culture supernatants of Bordetella pertussis are a brilliant yellow; however, the structure and biological role of the responsible pigment have not been investigated. In this study, a brilliant yellow-colored fraction was extracted from culture supernatants of B. pertussis and analyzed by HPLC. UV-visible spectral analysis and mass spectrometry identified the brilliant yellow pigment as riboflavin. Riboflavin production was high in lag and early log phases and riboflavin was found to enhance growth of B. pertussis in low-density cultures...
September 14, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28867343/assessment-of-clinical-symptoms-in-household-contacts-of-confirmed-pertussis-cases
#14
Angela Domínguez, Núria Soldevila, Joan A Caylà, Manuel García-Cenoz, Glòria Ferrús, Maria Rosa Sala-Farré, Josep Álvarez, Mònica Carol, Irene Barrabeig, Neus Camps, Lorena Coronas, Carmen Muñoz-Almagro, Pere Godoy
OBJECTIVES: We assessed the value of the clinical symptoms included in the case definition of pertussis in household contacts of laboratory-confirmed cases. METHODS: A prospective epidemiological study was made in two Spanish regions. Household contacts were identified for each confirmed case reported during 2012 and 2013. Two clinical samples were taken to determine the presence or absence of Bordetella pertussis by culture or real-time PCR. Clinical variables, age and vaccination status were recorded...
September 1, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28715411/a-curated-genome-scale-metabolic-model-of-bordetella-pertussis-metabolism
#15
Nick Fyson, Jerry King, Thomas Belcher, Andrew Preston, Caroline Colijn
The Gram-negative bacterium Bordetella pertussis is the causative agent of whooping cough, a serious respiratory infection causing hundreds of thousands of deaths annually worldwide. There are effective vaccines, but their production requires growing large quantities of B. pertussis. Unfortunately, B. pertussis has relatively slow growth in culture, with low biomass yields and variable growth characteristics. B. pertussis also requires a relatively expensive growth medium. We present a new, curated flux balance analysis-based model of B...
July 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28622957/real-time-polymerase-chain-reaction-based-detection-of-bordetella-pertussis-in-mexican-infants-and-their-contacts-a-3-year-multicenter-study
#16
MULTICENTER STUDY
Alejandra Aquino-Andrade, Gabriel Martínez-Leyva, Jocelin Mérida-Vieyra, Patricia Saltigeral, Antonino Lara, Wendy Domínguez, Silvestre García de la Puente, Agustín De Colsa
OBJECTIVES: To evaluate the usefulness of real-time polymerase chain reaction (RT-PCR) as a diagnostic method for the detection of Bordetella pertussis in hospitalized patients aged <1 year with a clinical diagnosis of whooping cough, as well as to identify the role of household contacts as a source of infection. STUDY DESIGN: This was a prospective, multicenter study of infants aged <1 year who were hospitalized with symptoms suggestive of whooping cough...
September 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/28538850/-infection-by-bordetella-pertussis-and-bordetella-parapertussis-in-cases-of-suspected-whooping-cough-2011-2015-in-mar-del-plata-argentina
#17
Silvina Lavayén, Claudia Zotta, Marcela Cepeda, Claudia Lara, Analia Rearte, Mabel Regueira
Objectives: . To classify the study population in the Argentinian National Health Surveillance System framework, determine the proportion of infection by Bordetella pertussis and Bordetella parapertussis, and identify factors associated with the cases of suspected whooping cough attended to in the city of Mar del Plata and its outskirts during the period 2011- 2015. Materials and Methods: An observational and descriptive study was carried out. Clinical cases with suspicion of whooping cough were diagnosed by laboratory...
January 2017: Revista Peruana de Medicina Experimental y Salud Pública
https://www.readbyqxmd.com/read/28511667/epidemiology-of-pertussis-in-casablanca-morocco-contribution-of-conventional-and-molecular-diagnosis-tools
#18
Khalid Katfy, Nicole Guiso, Idrissa Diawara, Khalid Zerouali, Bouchra Slaoui, Zineb Jouhadi, Abdelhadi Zineddine, Houria Belabbes, Naima Elmdaghri
BACKGROUND: Pertussis, a vaccine preventable disease, is still responsible of significant morbidity and mortality around the world, mostly in newborns. The aim of the present study was (1) to introduce pertussis surveillance in the major pediatric hospital of Casablanca (2) to analyze the prevalence of pertussis among children under 14 years of age and their entourage in Casablanca, Morocco. METHODS: This is a prospective and non-case controlled study, including children suspected of Pertussis admitted at the Abderrahim Harouchi Pediatric Hospital in Casablanca, from January 2013 to June 2015...
May 16, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28277139/sphingosine-1-phosphate-activates-the-akt-pathway-to-inhibit-chemotherapy-induced-human-granulosa-cell-apoptosis
#19
Shuyi Li, Jianling Chen, Xiaoling Fang, Xiaomeng Xia
To investigate whether sphingosine-1-phosphate (S1P), an apoptosis-inhibitor would be able to inhibit chemotherapy induced human granulosa cell apoptosis. Cultures of primary granulosa cells were isolated from women undergoing in vitro fertilization (IVF). MTT assay was used to measure the optimum concentration of CTX and S1P acts on human granulosa cells. Granulosa cells were added with pertussis toxin (PTX), the PI3K inhibitor LY294002. Western blot analysis was used to analyze the signaling pathway of proteins and cell apoptosis...
February 21, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28242451/characterisation-of-the-bordetella-pertussis-secretome-under-different-media
#20
Laurence Don Wai Luu, Sophie Octavia, Ling Zhong, Mark Raftery, Vitali Sintchenko, Ruiting Lan
Our understanding of the Bordetella pertussis secretome remains limited including the role of different growth conditions in the secretome. In this study the secretome of L1423, a clinical isolate from the 2008-2012 Australian epidemic, cultured on Stainer-Scholte (SS) and Thalen-IJssel (THIJS) media for 12h was characterised using liquid chromatography-mass spectrometry (LC-MS/MS). In the supernatant, LC-MS/MS identified 260 proteins with 143 bioinformatically predicted to be secreted. Eighty percent of proteins were identified in both media...
March 31, 2017: Journal of Proteomics
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