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https://www.readbyqxmd.com/read/27915049/csn1201-a-subunit-of-the-cop9-signalosome-regulates-the-virulence-in-cryptococcus-neoformans-infection
#1
Yibin Fan, Xiaohua Tao, Weili Pan, Wei Fang, Youming Huang, Mingyan Jia
The COP9 signalosome (CSN) is a multisubunit protein complex, and it now has been found to participate in diverse cellular and developmental processes in various eukaryotic organisms. Cryptococcus neoformans (C. neoformans) is an important basidiomycete pathogen that causes life-threatening meningoencephalitis primarily in the immune compromised population. Here, we generated CSN deletion mutants to investigate the role in Cryptococcus infection. Compared to other CSN mutants, we identified a CSN1201 mutant exhibited severely attenuated virulence...
November 30, 2016: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/27871853/nur77-downregulation-triggers-pulmonary-artery-smooth-muscle-cell-proliferation-and-migration-in-mice-with-hypoxic-pulmonary-hypertension-via-the-axin2-%C3%AE-catenin-signaling-pathway
#2
Xiaowei Nie, Jianxin Tan, Youai Dai, Wenjun Mao, Yuan Chen, Guowei Qin, Guirong Li, Chenyou Shen, Jingjing Zhao, Jingyu Chen
Pulmonary arterial hypertension (PAH) is a life-threatening disease characterized by remodeling of the pulmonary vasculature, including marked proliferation and reduced apoptosis of pulmonary artery smooth muscle cells (PASMCs). Members of the nuclear receptor 4A (NR4A) subfamily are involved in a variety of biological events, such as cell apoptosis, proliferation, inflammation, and metabolism. Activation of Nur77 (an orphan nuclear receptor that belongs to NR4A subfamily) has recently been reported to be as a beneficial agent in the treatment of cardiovascular and metabolic diseases...
November 15, 2016: Vascular Pharmacology
https://www.readbyqxmd.com/read/27869306/roles-of-multifunctional-cop9-signalosome-complex-in-cell-fate-and-implications-for-drug-discovery
#3
REVIEW
Ping Li, Longxiang Xie, Yinzhong Gu, Jiang Li, Jianping Xie
The eight subunits containing COP9 signalosome (CSN) complex, is highly conserved among eukaryotes. CSN, identified as a negative regulator of photomorphogenesis, has also been demonstrated to be important in proteolysis, cellular signal transduction and cell cycle regulation in various eukaryotic organisms. This review mainly summarizes the roles of CSN in cell cycle regulation, signal transduction and apoptosis, and its potential as diagnostic biomarkers, drug targets for cancer and infectious diseases. This article is protected by copyright...
November 21, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27861949/absolute-quantification-of-micrornas-in-green-tea-camellia-sinensis-by-stem-loop-quantitative-real-time-pcr
#4
Ying-Hui Hou, Anburaj Jeyaraj, Xiao Zhang, Chao-Ling Wei
BACKGROUND: There are some studies to show that food-derived plant microRNAs (miRNAs) may be detected in mammals. The research evidence has being provoked a considerable debate whether plant-derived miRNAs exert the same regulatory functions as endogenous animal miRNAs. To test the hypothesis, the methods of highly sensitive absolute quantification miRNAs have been developed. However, absolute miRNA quantification of green tea has not yet been reported. In this study, it is the first to build an absolute quantification method to detect miRNAs level in green tea using stem-loop quantitative real-time PCR (qRT-PCR)...
November 12, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27836030/validation-of-a-new-technique-to-detect-cryptosporidium-spp-oocysts-in-bovine-feces
#5
Sandra Valéria Inácio, Jancarlo Ferreira Gomes, Bruno César Miranda Oliveira, Alexandre Xavier Falcão, Celso Tetsuo Nagase Suzuki, Bianca Martins Dos Santos, Monally Conceição Costa de Aquino, Rafaela Silva de Paula Ribeiro, Danilla Mendes de Assunção, Pamella Almeida Freire Casemiro, Marcelo Vasconcelos Meireles, Katia Denise Saraiva Bresciani
Due to its important zoonotic potential, cryptosporidiosis arouses strong interest in the scientific community, because, it was initially considered a rare and opportunistic disease. The parasitological diagnosis of the causative agent of this disease, the protozoan Cryptosporidium spp., requires the use of specific techniques of concentration and permanent staining, which are laborious and costly, and are difficult to use in routine laboratory tests. In view of the above, we conducted the feasibility, development, evaluation and intralaboratory validation of a new parasitological technique for analysis in optical microscopy of Cryptosporidium spp...
November 1, 2016: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/27833851/overexpression-of-cop9-signalosome-subunits-csn7a-and-csn7b-exerts-different-effects-on-adipogenic-differentiation
#6
Xiaohua Huang, Jürgen Ordemann, Johann Pratschke, Wolfgang Dubiel
The COP9 signalosome (CSN) is an essential regulator of cullin-RING-ubiquitin (Ub) ligases (CRLs), which ubiquitinate important cellular regulators and target them for degradation by the Ub proteasome system (UPS). The CSN exhibits deneddylating activity localized on subunit CSN5, which removes the ubiquitin-like protein Nedd8 from the cullins of CRLs. CSN-mediated deneddylation is an important step in the process of CRL remodeling, in which new substrate recognition units are incorporated into Ub ligases to meet changed requirements for proteolysis in cells...
November 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/27774986/targeted-inhibition-of-the-cop9-signalosome-for-treatment-of-cancer
#7
Anita Schlierf, Eva Altmann, Jean Quancard, Anne B Jefferson, René Assenberg, Martin Renatus, Matthew Jones, Ulrich Hassiepen, Michael Schaefer, Michael Kiffe, Andreas Weiss, Christian Wiesmann, Richard Sedrani, Jörg Eder, Bruno Martoglio
The COP9 signalosome (CSN) is a central component of the activation and remodelling cycle of cullin-RING E3 ubiquitin ligases (CRLs), the largest enzyme family of the ubiquitin-proteasome system in humans. CRLs are implicated in the regulation of numerous cellular processes, including cell cycle progression and apoptosis, and aberrant CRL activity is frequently associated with cancer. Remodelling of CRLs is initiated by CSN-catalysed cleavage of the ubiquitin-like activator NEDD8 from CRLs. Here we describe CSN5i-3, a potent, selective and orally available inhibitor of CSN5, the proteolytic subunit of CSN...
October 24, 2016: Nature Communications
https://www.readbyqxmd.com/read/27748879/quercetin-induced-apoptosis-of-ht-29%C3%A2-colon-cancer-cells-via-inhibition-of-the-akt-csn6-myc-signaling-axis
#8
Lin Yang, Yanqing Liu, Mei Wang, Yayun Qian, Xiaoyun Dong, Hao Gu, Haibo Wang, Shiyu Guo, Tadashi Hisamitsu
Constitutive photomorphogenesis 9 signalosome (CSN) consists of a total of eight subunits (CSN1-CSN8) in mammalian cells. CSN6 may promote carcinogenesis by positively regulating v‑myc avian myelocytomatosis viral oncogene homolog (Myc) and MDM2 proto‑oncogene stability, and is regarded as a potential target for cancer therapy. Quercetin has a substantial anticancer effect on various human cancer cells. The present study investigated the effects of quercetin on HT-29 human colorectal cancer cell viability, apoptosis and cell cycle arrest using an MTT assay, flow cytometry, transmission electron microscopy and western blotting...
November 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27744526/functional-abolition-of-carotid-body-activity-restores-insulin-action-and-glucose-homeostasis-in-rats-key-roles-for-visceral-adipose-tissue-and-the-liver
#9
Joana F Sacramento, Maria J Ribeiro, Tiago Rodrigues, Elena Olea, Bernardete F Melo, Maria P Guarino, Rui Fonseca-Pinto, Cristiana R Ferreira, Joana Coelho, Ana Obeso, Raquel Seiça, Paulo Matafome, Sílvia V Conde
AIMS/HYPOTHESIS: We recently described that carotid body (CB) over-activation is involved in the aetiology of insulin resistance and arterial hypertension in animal models of the metabolic syndrome. Additionally, we have demonstrated that CB activity is increased in animal models of insulin resistance, and that carotid sinus nerve (CSN) resection prevents the development of insulin resistance and arterial hypertension induced by high-energy diets. Here, we tested whether the functional abolition of CB by CSN transection would reverse pre-established insulin resistance, dyslipidaemia, obesity, autonomic dysfunction and hypertension in animal models of the metabolic syndrome...
January 2017: Diabetologia
https://www.readbyqxmd.com/read/27729873/neonatal-maternal-separation-augments-carotid-body-response-to-hypoxia-in-adult-males-but-not-female-rats
#10
Jorge Soliz, Rose Tam, Richard Kinkead
Perinatal exposure to adverse experiences disrupts brain development, including the brainstem network that regulates breathing. At adulthood, rats previously subjected to stress (in the form of neonatal maternal separation; NMS) display features reported in patients suffering from sleep disordered breathing, including an increased hypoxic ventilatory response and hypertension. This effect is also sex-specific (males only). Based on these observations, we hypothesized that NMS augments the carotid body's O2-chemosensitivity...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27679973/nur77-exacerbates-pc12-cellular-injury-in-vitro-by-aggravating-mitochondrial-impairment-and-endoplasmic-reticulum-stress
#11
Huimin Gao, Zhaoyu Chen, Yongmei Fu, Xiaoyan Yang, Ruihui Weng, Rui Wang, Jianjun Lu, Mengqiu Pan, Kunlin Jin, Chris McElroy, Beisha Tang, Ying Xia, Qing Wang
The nuclear orphan receptor, Nur77 plays important roles in neuroimflammation, apoptosis, and dopaminergic neurodegeneration. We conducted a further mechanistic investigation into the association of Nur77 with cell death. Cytosporone B (Csn-B), an agonist for Nur77, and Nur77 knockdown were adopted in the 6-hydroxydopamine (OHDA)-lesioned PC12 cells to investigate the mechanisms underlying Nur77-mediated injury. The 6-OHDA incubation caused Nur77 translocation from the nucleus to cytosol and Endoplasm reticulum (ER) and induced co-localization of Tom20/Nur77 and Protein Disulfide Isomerase (PDI)/Nur77...
September 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27636714/accelerated-bony-defect-healing-based-on-chitosan-thermosensitive-hydrogel-scaffolds-embedded-with-chitosan-nanoparticles-for-the-delivery-of-bmp2-plasmid-dna
#12
Hui Li, Qiuxia Ji, Ximin Chen, Yan Sun, Quanchen Xu, Panpan Deng, Fang Hu, Jianjun Yang
Regeneration of alveolar bone is among the main purposes of periodontal treatment. However, it is a challenge for both dentists and patients. An injectable chitosan-based thermosensitive hydrogel scaffold (CS/CSn-GP) was successfully designed and prepared with the incorporation of bone morphogenetic protein-2 plasmid DNA (pDNA-BMP2)-loaded chitosan nanoparticles (CS/CSn(pDNA-BMP2)-GP) in this study. The aim of this study was to evaluate the regenerative effect of this scaffold on the endogenous repair of alveolar bone...
January 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/27626169/the-putative-oncotarget-csn5-controls-a-transcription-uncorrelated-p53-mediated-autophagy-implicated-in-cancer-cell-survival-under-curcumin-treatment
#13
Qing-Yu Zhang, Rui Jin, Xian Zhang, Ji-Po Sheng, Fang Yu, Ren-Xiang Tan, Ying Pan, Jun-Jian Huang, Ling-Dong Kong
Curcumin has shown promise as a safe and specific anticancer agent. The COP9 signalosome (CSN) component CSN5, a known specific target for curcumin, can control p53 stability by increasing its degradation through ubiquitin system. But the correlation of CSN5-controlled p53 to anticancer therapeutic effect of curcumin is currently unknown. Here we showed that CSN5-controlled p53 was transcriptional inactive and responsible for autophagy in human normal BJ cells and cancer HepG2 cells under curcumin treatment...
September 10, 2016: Oncotarget
https://www.readbyqxmd.com/read/27613030/immunodepletion-and-immunopurification-as-approaches-for-csn-research
#14
Amnon Golan, Ning Wei, Elah Pick
The COP9 signalosome (CSN) is an evolutionary conserved complex that is found in all eukaryotes, and implicated in regulating the activity of Cullin-RING ubiquitin Ligases (CRLs). Activity of CRLs is highly regulated; complexes are active when the cullin subunit is covalently attached to the ubiquitin like modifier, Nedd8. Neddylation/deneddylation cycles are required for proper CRLs activity, and deneddylation is performed by the CSN complex.We describe here a method utilizing resin-coupled antibodies to deplete the CSN from human cell extracts, and to obtain endogenous CSN complexes by immunopurification...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27603573/uncovering-cortico-striatal-correlates-of-cognitive-fatigue-in-pediatric-acquired-brain-disorder-evidence-from-traumatic-brain-injury
#15
Nicholas P Ryan, Miriam H Beauchamp, Richard Beare, Lee Coleman, Michael Ditchfield, Michael Kean, Timothy J Silk, Sila Genc, Cathy Catroppa, Vicki A Anderson
Cognitive fatigue is among the most profound and disabling sequelae of pediatric acquired brain disorders, however the neural correlates of these symptoms in children remains unexplored. One hypothesis suggests that cognitive fatigue may arise from dysfunction of cortico-striatal networks (CSNs) implicated in effort output and outcome valuation. Using pediatric traumatic brain injury (TBI) as a model, this study investigated (i) the sub-acute effect of brain injury on CSN volume; and (ii) potential relationships between cognitive fatigue and sub-acute volumetric abnormalities of the CSN...
October 2016: Cortex; a Journal Devoted to the Study of the Nervous System and Behavior
https://www.readbyqxmd.com/read/27600773/nr4a1-dependent-ly6c-low-monocytes-contribute-to-reducing-joint-inflammation-in-arthritic-mice-through-treg-cells
#16
Alexandre Brunet, Manon LeBel, Benoit Egarnes, Carine Paquet-Bouchard, Anne-Julie Lessard, Jacques P Brown, Jean Gosselin
Monocytes are central to the physiopathology of arthritis, but their roles in progression and resolution of the disease remain to be clarified. Using NR4A1(-/-) mice, which lack patrolling lymphocyte antigen 6C (Ly6C(low) ) monocytes, we found that inflammatory Ly6C(high) monocytes contribute to rapid development of arthritis in a serum transfer-induced arthritis (STIA) model. Our experiments suggest that patrolling monocytes do not promote the initiation and progression of arthritis in mice, as severity of symptoms was amplified in NR4A1(-/-) mice...
September 7, 2016: European Journal of Immunology
https://www.readbyqxmd.com/read/27581057/a-wheat-cop9-subunit-5-like-gene-is-negatively-involved-in-host-response-to-leaf-rust
#17
Hongtao Zhang, Xiaojing Wang, Michael J Giroux, Li Huang
The COP9 (constitutive photomorphogenesis 9) signalosome (CSN) is a protein complex involved in the ubiquitin proteasome system and a common host target of diverse pathogens in Arabidopsis. The known derubylation function of the COP9 complex is carried out by subunit 5 encoded by AtCSN5A or AtCSN5B in Arabidopsis. A single CSN5-like gene (designated as TaCSN5) with three homeologues was identified on the long arms of wheat (Triticum aestivum L.) group 2 chromosomes. In this study, we identified and characterized the function of TaCSN5 in response to infection by the leaf rust pathogen...
August 31, 2016: Molecular Plant Pathology
https://www.readbyqxmd.com/read/27569242/the-chinese-expert-consensus-on-evaluation-of-coma-after-cardiopulmonary-resuscitation
#18
(no author information available yet)
No abstract text is available yet for this article.
2016: Chinese Medical Journal
https://www.readbyqxmd.com/read/27546621/csn6-controls-the-proliferation-and-metastasis-of-glioblastoma-by-chip-mediated-degradation-of-egfr
#19
J Hou, Q Deng, J Zhou, J Zou, Y Zhang, P Tan, W Zhang, H Cui
CSN6, a critical subunit of the constitutive photomorphogenesis 9 (COP9) signalosome (CSN), has received attention as a regulator of the degradation of cancer-related proteins such as p53, c-myc and c-Jun, through the ubiquitin-proteasome system, suggesting its importance in cancerogenesis. However, the biological functions and molecular mechanisms of CSN6 in glioblastoma (GBM) remain poorly understood. Here, we report that GBM tumors overexpressed CSN6 compared with normal brain tissues and that CSN6 promoted GBM cell proliferation, migration, invasion and tumorigenesis...
August 22, 2016: Oncogene
https://www.readbyqxmd.com/read/27516938/ompa-signal-peptide-leads-to-heterogenous-secretion-of-b-subtilis-chitosanase-enzyme-from-e-coli-expression-system
#20
Phornsiri Pechsrichuang, Chomphunuch Songsiriritthigul, Dietmar Haltrich, Sittiruk Roytrakul, Peenida Namvijtr, Napolean Bonaparte, Montarop Yamabhai
The production of secreted recombinant proteins from E. coli is pivotal to the biotechnological industry because it reduces the cost of downstream processing. Proteins destined for secretion contain an N-terminal signal peptide that is cleaved by secretion machinery in the plasma membrane. The resulting protein is released in an active mature form. In this study, Bacillus subtilis chitosanase (Csn) was used as a model protein to compare the effect of two signal peptides on the secretion of heterologous recombinant protein...
2016: SpringerPlus
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