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Wen Tian, Tianyou Wu, Rui Zhao, Jinhao Xu, Yao He, Hongrong Wang
The purpose of this study was to determine effects of jugular infusion of either balanced or imbalanced amino acid mixture on milk production and composition in dairy cows. Eight mid-lactation Holstein cows were randomly assigned to 5-d continuous jugular infusions of saline (CTL), essential amino acid (EAA) mixture prepared on the profile of casein (CSN, 160 g/d), EAA mixture excluding leucine (Leu) (-Leu, 163 g/d) or EAA mixture excluding arginine (Arg) (-Arg, 158 g/d) in a duplicated 4 × 4 Latin square design with 4 infusion periods separated by a 7-d interval period...
September 2017: Animal nutrition
Aamna Shah, Izhar Hussain, Ghulam Murtaza
This study provides the optimum preparation parameters for functional chitosan silver nanocomposite (CSN) films with promising antibacterial efficacy though prepared with very low silver nitrate (AgNO3 ) concentration. Chitosan nano‑silver composites were fabricated by In-situ chemical method utilizing the reducing ability of sodium borohydride (NaBH4 ) and afterward casted into films. Utilization of response surface methodology, NCSS, and SigmaPlot for the optimization of CSN and their predicted antibacterial efficacy assessment of the selected bacterial strains (standard and clinical) was the essential part of the study...
May 11, 2018: International Journal of Biological Macromolecules
Marie K Schwinn, Trish Hoang, Xiaofeng Yang, Xiansi Zhao, Jingya Ma, Ping Li, Keith V Wood, William D Mallender, Michael E Bembenek, Zhong-Hua Yan
Neddylation is a posttranslational modification that regulates protein stability, activity, and subcellular localization. Here, we describe a new tool for exploring the neddylation cycle of cullin1 (Cul1) directly in a cellular context. This assay utilizes the NanoLuc® Binary Technology (NanoBiT) to monitor the covalent neddylation status of Cul1. A stable clonal cell line derived from HEK293 was developed that expressed a C-terminus LgBiT tagged-Cul1 and N-terminus SmBiT tagged-Nedd8. Using this cell line, we screened inhibitors that are known to disrupt Nedd8 biology and demonstrated that both inhibitors of Nedd8-activating enzyme (NAE) and Constitutive photomorphogenesis 9 signalosome (CSN) complex produce concentration and time dependent signal decreases and increases, respectively...
May 5, 2018: Analytical Biochemistry
Dakai Xiao, Shengli Yang, Liyan Huang, Huiming He, Hui Pan, Jianxing He
Background: The COP9 signalosome (CSN) is an evolutionarily conserved complex composed of eight subunits (CSN1-CSN8). Among the CSN subunits, CSN5 and its dimerization partner CSN6 are the only two MPN (Mpr1-Pad1-N-terminal) domain-containing subunits. These two subunits play essential roles in a variety of biological processes, such as cell cycle progression, protein stability and signal transduction. However, their expression patterns and clinical significance in lung cancer are not completely clear...
March 2018: Journal of Thoracic Disease
Huihui Sun, Xiangzhao Mao, Na Guo, Ling Zhao, Rong Cao, Qi Liu
In the process of genome mining for novel chitosanases by phylogeny-based enzymatic product specificity prediction, a gene named Csn-PD from Paenibacillus dendritiformis was discovered. The enzyme was classified as a member of the GH46 family of glycoside hydrolase based on sequence alignment, and it was functionally expressed in Escherichia coli BL21 (DE3). The recombinant chitosanase was purified, and its molecular weight was estimated to be 31 kDa by SDS-PAGE. Csn-PD displayed maximal activity toward colloidal chitosan at pH 7...
April 24, 2018: Journal of Agricultural and Food Chemistry
Cem Simsek, Takashi Kojima, Murat Dogru, Kazuo Tsubota
Purpose: To investigate the morphologic changes in the corneal subbasal nerve (CSN) plexus in wild-type mice after exposure to environmental dry eye stress (EDES) using in vivo confocal microscopy (IVCM). Methods: We examined 22 eyes of 8-week-old wild-type male mice (Balb/c, n = 11). The mice were exposed to an air fan inside a small compartment 5 hours/day for 3 days (EDES). Aqueous tear secretion and corneal epithelial damage were assessed. The CSNs were investigated by laser-scanning IVCM...
April 1, 2018: Investigative Ophthalmology & Visual Science
Xinliu Zeng, Zhang Yue, Ying Gao, Guosong Jiang, Fuqing Zeng, Ying Shao, Jiayu Huang, Minuo Yin, Yajie Li
BACKGROUND/AIMS: Excess fibrosis may lead to chronic pain, scarring, and infertility as endometriosis develops and progresses. The pathogenesis of endometriosis has been linked to transforming growth factor-β (TGF-β), the most potent promoter of fibrosis. METHODS: Levels of NR4A1 and P-NR4A1 protein in human endometrial and endometriotic tissue were assessed by western blotting and immunohistochemistry. The expression levels of fibrotic markers in stromal cells were evaluated by real-time PCR...
April 13, 2018: Cellular Physiology and Biochemistry
Andrea Porzionato, Veronica Macchi, Carla Stecco, Raffaele De Caro
Interest has been renewed in the anatomy and physiology of the carotid sinus nerve (CSN) and its targets (carotid sinus and carotid body, CB), due to recent proposals of surgical procedures for a series of common pathologies, such as carotid sinus syndrome, hypertension, heart failure, and insulin resistance. The CSN originates from the glossopharyngeal nerve soon after its appearance from the jugular foramen. It shows frequent communications with the sympathetic trunk (usually at the level of the superior cervical ganglion) and the vagal nerve (main trunk, pharyngeal branches, or superior laryngeal nerve)...
April 16, 2018: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
Mingshuang Wang, Xiao Yang, Ruoxin Ruan, Huilan Fu, Hongye Li
The COP9 signalosome (CSN) is a highly conserved protein complex involved in the ubiquitin-proteasome system. Its metalloisopeptidase activity resides in subunit 5 (CSN5). Functions of csn5 in phytopathogenic fungi are poorly understood. Here, we knocked out the csn5 ortholog ( Aacsn5 ) in the tangerine pathotype of Alternaria alternata . The Δ Aacsn5 mutant showed a moderately reduced growth rate compared to the wildtype strain and was unable to produce conidia. The growth of Δ Aacsn5 mutant was not affected in response to oxidative and osmotic stresses...
2018: Frontiers in Microbiology
Huanhuan Li, Quansheng Chen, Md Mehedi Hassan, Qin Ouyang, Tianhui Jiao, Yi Xu, Min Chen
Mercury ion (Hg2+ ) is a highly hazardous and widespread pollutant with bio-accumulative properties. Although the existing Hg2+ detection methods have high sensitivity and reliability, whereas there have few reports concerning bimodal detection for Hg2+ with one sensor. Toward this goal, a novel sensor based on rhodamine derivatives (RhD) grafted AuNS@Ag core-shell nanocubes (CSN) has been synthesized and shown the bimodal detection capabilities with metal enhanced fluorescence (MEF) and surface enhanced Raman scattering (SERS) for Hg2+ ...
August 14, 2018: Analytica Chimica Acta
Jie Li, Yingjie Li, Bin Wang, Yongfu Ma, Ping Chen
CSN5/JAB1 is a critical subunit of the COP9 signalosome (CSN) and is essentially involved in diverse types of cancer, but little is known about the role of CSN5 in non-small cell lung cancer (NSCLC). In the current study, we found that CSN5 expression was higher in NSCLC tissues compared to the corresponding non-tumor tissues. High CSN5 expression level is closely correlated with tumor progression and poor survival in NSCLC patients. We also found that knockdown of CSN5 remarkably suppressed cell growth by inducing cell cycle arrest and apoptosis promotion in NSCLC cells...
March 26, 2018: Biochemical and Biophysical Research Communications
V Moreno, J Bach, M Zarroca, Ll Font, C Roqué, R Linares
Radon levels in the soil and groundwater in the North Maladeta Fault area (located in the Aran Valley sector, Central Pyrenees) are analysed from both geological and radiation protection perspectives. This area is characterized by the presence of two important normal faults: the North Maladeta fault (NMF) and the Tredós Fault (TF). Two primary aspects make this study interesting: (i) the NMF shows geomorphic evidence of neotectonic activity and (ii) the presence of a thermal spa, Banhs de Tredós, which exploits one of the several natural springs of the area and needs to be evaluated for radiation dosing from radon according to the European regulation on basic safety standards for protection against ionizing radiation...
September 2018: Journal of Environmental Radioactivity
Karine C Flor, Elaine F Silva, Miguel F Menezes, Gustavo R Pedrino, Eduardo Colombari, Daniel B Zoccal
Exposure to chronic sustained hypoxia (SH), as experienced in high altitudes, elicits an increase in ventilation, named ventilatory acclimatization to hypoxia (VAH). We previously showed that rats exposed to short-term (24 h) SH exhibit enhanced abdominal expiratory motor activity at rest, accompanied by augmented baseline sympathetic vasoconstrictor activity. In the present study, we investigated whether the respiratory and sympathetic changes elicited by short-term SH are accompanied by carotid body chemoreceptor sensitization...
2018: Frontiers in Physiology
Colin A Nurse, Erin M Leonard, Shaima Salman
Mammalian carotid bodies (CB) are chemosensory organs that mediate compensatory cardiorespiratory reflexes in response to low blood PO2 (hypoxemia) and elevated CO2 /H+ (acid hypercapnia). The chemoreceptors are glomus or type I cells that occur in clusters enveloped by neighboring glial-like type II cells. During chemoexcitation type I cells depolarize, leading to Ca2+ -dependent release of several neurotransmitters, some excitatory and others inhibitory, that help shape the afferent carotid sinus nerve (CSN) discharge...
April 1, 2018: Physiological Genomics
Chance Dumaine, Mercedeh Kiaii, Lisa Miller, Louise Moist, Matthew J Oliver, Charmaine E Lok, Swapnil Hiremath, Jennifer M MacRae
Background: One of the mandates of the Canadian Society of Nephrology's (CSN) Vascular Access Working Group (VAWG) is to inform the nephrology community of the current status of vascular access (VA) practice within Canada. To better understand VA practice patterns across Canada, the CSN VAWG conducted a national survey. Objectives: (1) To inform on VA practice patterns, including fistula creation and maintenance, within Canada. (2) To determine the degree of consensus among Canadian clinicians regarding patient suitability for fistula creation and to assess barriers to and facilitators of fistula creation in Canada...
2018: Canadian Journal of Kidney Health and Disease
Dan Jin, Ming Wu, Bosheng Li, Birte Bücker, Philipp Keil, Shaoman Zhang, Jigang Li, Dingming Kang, Jie Liu, Jie Dong, Xing Wang Deng, Vivian Irish, Ning Wei
The control of seed germination and seed dormancy are critical for the successful propagation of plant species, and are important agricultural traits. Seed germination is tightly controlled by the balance of gibberellin (GA) and abscisic acid (ABA), and is influenced by environmental factors. The COP9 Signalosome (CSN) is a conserved multi-subunit protein complex that is best known as a regulator of the Cullin-RING family of ubiquitin E3 ligases (CRLs). Multiple viable mutants of the CSN showed poor germination, except for csn5b-1...
February 2018: PLoS Genetics
Jae-Min Park, Se Jin Jang, Sang-Ick Lee, Won-Jun Lee
We designed cyclosilazane-type silicon precursors and proposed a three-step plasma-enhanced atomic layer deposition (PEALD) process to prepare silicon nitride films with high quality and excellent step coverage. The cyclosilazane-type precursor, 1,3-di-isopropylamino-2,4-dimethylcyclosilazane (CSN-2), has a closed ring structure for good thermal stability and high reactivity. CSN-2 showed thermal stability up to 450 °C and a sufficient vapor pressure of 4 Torr at 60 °C. The energy for the chemisorption of CSN-2 on the undercoordinated silicon nitride surface as calculated by density functional theory method was -7...
March 14, 2018: ACS Applied Materials & Interfaces
Qingqing Liu, Yike Zhou, Ruiqi Tang, Xuehong Wang, Qiwen Hu, Ying Wang, Qun He
No abstract text is available yet for this article.
February 15, 2018: Molecular and Cellular Biology
Judith C Chow, John G Watson, Mark C Green, Xiaoliang Wang, L-W Antony Chen, Dana L Trimble, Paul M Cropper, Steven D Kohl, Steven B Gronstal
The replacement of the DRI Model 2001 with Model 2015 thermal/optical analyzers (TOAs) results in continuity of the long-term organic carbon (OC) and elemental carbon (EC) data base, and it adds optical information with no additional carbon analysis effort. The value of multiwavelength light attenuation is that light absorption due to black carbon (BC) can be separated from that of brown carbon (BrC), with subsequent attribution to known sources such as biomass burning and secondary organic aerosols. There is evidence of filter loading effects for the 25% of all samples with the highest EC concentrations based on the ratio of light attenuation to EC...
January 17, 2018: Journal of the Air & Waste Management Association
Justyna Korczeniewska, Betsy J Barnes
The transcription factor interferon regulatory factor 5 (IRF5) exerts crucial functions in the regulation of host immunity against extracellular pathogens, DNA damage-induced apoptosis, death receptor signaling, and macrophage polarization. Tight regulation of IRF5 is thus warranted for an efficient response to extracellular stressors and for limiting autoimmune and inflammatory responses. Here we report that the COP9 signalosome (CSN), a general modulator of diverse cellular and developmental processes, associates constitutively with IRF5 and promotes its protein stability...
February 1, 2018: Molecular and Cellular Biology
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