Read by QxMD icon Read


Lin Yang, Yanqing Liu, Mei Wang, Yayun Qian, Xiaoyun Dong, Hao Gu, Haibo Wang, Shiyu Guo, Tadashi Hisamitsu
Constitutive photomorphogenesis 9 signalosome (CSN) consists of a total of eight subunits (CSN1-CSN8) in mammalian cells. CSN6 may promote carcinogenesis by positively regulating v‑myc avian myelocytomatosis viral oncogene homolog (Myc) and MDM2 proto‑oncogene stability, and is regarded as a potential target for cancer therapy. Quercetin has a substantial anticancer effect on various human cancer cells. The present study investigated the effects of quercetin on HT-29 human colorectal cancer cell viability, apoptosis and cell cycle arrest using an MTT assay, flow cytometry, transmission electron microscopy and western blotting...
October 10, 2016: Molecular Medicine Reports
Joana F Sacramento, Maria J Ribeiro, Tiago Rodrigues, Elena Olea, Bernardete F Melo, Maria P Guarino, Rui Fonseca-Pinto, Cristiana R Ferreira, Joana Coelho, Ana Obeso, Raquel Seiça, Paulo Matafome, Sílvia V Conde
AIMS/HYPOTHESIS: We recently described that carotid body (CB) over-activation is involved in the aetiology of insulin resistance and arterial hypertension in animal models of the metabolic syndrome. Additionally, we have demonstrated that CB activity is increased in animal models of insulin resistance, and that carotid sinus nerve (CSN) resection prevents the development of insulin resistance and arterial hypertension induced by high-energy diets. Here, we tested whether the functional abolition of CB by CSN transection would reverse pre-established insulin resistance, dyslipidaemia, obesity, autonomic dysfunction and hypertension in animal models of the metabolic syndrome...
October 16, 2016: Diabetologia
Jorge Soliz, Rose Tam, Richard Kinkead
Perinatal exposure to adverse experiences disrupts brain development, including the brainstem network that regulates breathing. At adulthood, rats previously subjected to stress (in the form of neonatal maternal separation; NMS) display features reported in patients suffering from sleep disordered breathing, including an increased hypoxic ventilatory response and hypertension. This effect is also sex-specific (males only). Based on these observations, we hypothesized that NMS augments the carotid body's O2-chemosensitivity...
2016: Frontiers in Physiology
Huimin Gao, Zhaoyu Chen, Yongmei Fu, Xiaoyan Yang, Ruihui Weng, Rui Wang, Jianjun Lu, Mengqiu Pan, Kunlin Jin, Chris McElroy, Beisha Tang, Ying Xia, Qing Wang
The nuclear orphan receptor, Nur77 plays important roles in neuroimflammation, apoptosis, and dopaminergic neurodegeneration. We conducted a further mechanistic investigation into the association of Nur77 with cell death. Cytosporone B (Csn-B), an agonist for Nur77, and Nur77 knockdown were adopted in the 6-hydroxydopamine (OHDA)-lesioned PC12 cells to investigate the mechanisms underlying Nur77-mediated injury. The 6-OHDA incubation caused Nur77 translocation from the nucleus to cytosol and Endoplasm reticulum (ER) and induced co-localization of Tom20/Nur77 and Protein Disulfide Isomerase (PDI)/Nur77...
September 29, 2016: Scientific Reports
Hui Li, Qiuxia Ji, Yan Sun, Quanchen Xu, Wenbin Song, Wei Li, Dandan Li, Panpan Deng, Fang Hu, Jianjun Yang
Regeneration of alveolar bone is among the main purposes of periodontal treatment. However, it is a challenge for both dentists and patients. An injectable chitosan-based thermosensitive hydrogel scaffold (CS/CSn-GP) was successfully designed and prepared with the incorporation of bone morphogenetic protein-2 plasmid DNA (pDNA-BMP2)-loaded chitosan nanoparticles (CS/CSn(pDNA-BMP2)-GP) in this study. The aim of this study was to evaluate the regenerative effect of this scaffold on the endogenous repair of alveolar bone...
September 16, 2016: Journal of Biomedical Materials Research. Part A
Qing-Yu Zhang, Rui Jin, Xian Zhang, Ji-Po Sheng, Fang Yu, Ren-Xiang Tan, Ying Pan, Jun-Jian Huang, Ling-Dong Kong
Curcumin has shown promise as a safe and specific anticancer agent. The COP9 signalosome (CSN) component CSN5, a known specific target for curcumin, can control p53 stability by increasing its degradation through ubiquitin system. But the correlation of CSN5-controlled p53 to anticancer therapeutic effect of curcumin is currently unknown. Here we showed that CSN5-controlled p53 was transcriptional inactive and responsible for autophagy in human normal BJ cells and cancer HepG2 cells under curcumin treatment...
September 10, 2016: Oncotarget
Amnon Golan, Ning Wei, Elah Pick
The COP9 signalosome (CSN) is an evolutionary conserved complex that is found in all eukaryotes, and implicated in regulating the activity of Cullin-RING ubiquitin Ligases (CRLs). Activity of CRLs is highly regulated; complexes are active when the cullin subunit is covalently attached to the ubiquitin like modifier, Nedd8. Neddylation/deneddylation cycles are required for proper CRLs activity, and deneddylation is performed by the CSN complex.We describe here a method utilizing resin-coupled antibodies to deplete the CSN from human cell extracts, and to obtain endogenous CSN complexes by immunopurification...
2016: Methods in Molecular Biology
Nicholas P Ryan, Miriam H Beauchamp, Richard Beare, Lee Coleman, Michael Ditchfield, Michael Kean, Timothy J Silk, Sila Genc, Cathy Catroppa, Vicki A Anderson
Cognitive fatigue is among the most profound and disabling sequelae of pediatric acquired brain disorders, however the neural correlates of these symptoms in children remains unexplored. One hypothesis suggests that cognitive fatigue may arise from dysfunction of cortico-striatal networks (CSNs) implicated in effort output and outcome valuation. Using pediatric traumatic brain injury (TBI) as a model, this study investigated (i) the sub-acute effect of brain injury on CSN volume; and (ii) potential relationships between cognitive fatigue and sub-acute volumetric abnormalities of the CSN...
October 2016: Cortex; a Journal Devoted to the Study of the Nervous System and Behavior
Alexandre Brunet, Manon LeBel, Benoit Egarnes, Carine Paquet-Bouchard, Anne-Julie Lessard, Jacques P Brown, Jean Gosselin
Monocytes are central to the physiopathology of arthritis, but their roles in progression and resolution of the disease remain to be clarified. Using NR4A1(-/-) mice, which lack patrolling lymphocyte antigen 6C (Ly6C(low) ) monocytes, we found that inflammatory Ly6C(high) monocytes contribute to rapid development of arthritis in a serum transfer-induced arthritis (STIA) model. Our experiments suggest that patrolling monocytes do not promote the initiation and progression of arthritis in mice, as severity of symptoms was amplified in NR4A1(-/-) mice...
September 7, 2016: European Journal of Immunology
Hongtao Zhang, Xiaojing Wang, Michael J Giroux, Li Huang
The COP9 (constitutive photomorphogenesis 9) signalosome (CSN) is a protein complex involved in the ubiquitin proteasome system and a common host target of diverse pathogens in Arabidopsis. The known derubylation function of the COP9 complex is carried out by subunit 5 encoded by AtCSN5A or AtCSN5B in Arabidopsis. A single CSN5-like gene (designated as TaCSN5) with three homeologues was identified on the long arms of wheat (Triticum aestivum L.) group 2 chromosomes. In this study, we identified and characterized the function of TaCSN5 in response to infection by the leaf rust pathogen...
August 31, 2016: Molecular Plant Pathology
(no author information available yet)
No abstract text is available yet for this article.
2016: Chinese Medical Journal
J Hou, Q Deng, J Zhou, J Zou, Y Zhang, P Tan, W Zhang, H Cui
CSN6, a critical subunit of the constitutive photomorphogenesis 9 (COP9) signalosome (CSN), has received attention as a regulator of the degradation of cancer-related proteins such as p53, c-myc and c-Jun, through the ubiquitin-proteasome system, suggesting its importance in cancerogenesis. However, the biological functions and molecular mechanisms of CSN6 in glioblastoma (GBM) remain poorly understood. Here, we report that GBM tumors overexpressed CSN6 compared with normal brain tissues and that CSN6 promoted GBM cell proliferation, migration, invasion and tumorigenesis...
August 22, 2016: Oncogene
Phornsiri Pechsrichuang, Chomphunuch Songsiriritthigul, Dietmar Haltrich, Sittiruk Roytrakul, Peenida Namvijtr, Napolean Bonaparte, Montarop Yamabhai
The production of secreted recombinant proteins from E. coli is pivotal to the biotechnological industry because it reduces the cost of downstream processing. Proteins destined for secretion contain an N-terminal signal peptide that is cleaved by secretion machinery in the plasma membrane. The resulting protein is released in an active mature form. In this study, Bacillus subtilis chitosanase (Csn) was used as a model protein to compare the effect of two signal peptides on the secretion of heterologous recombinant protein...
2016: SpringerPlus
Xin Xu, Qun Lin Chan, Saima Hilal, Mohammad Kamran Ikram, Narayanaswamy Venketasubramanian, Boon Yeow Tan, Yanhong Dong, Christopher Li-Hsian Chen, Simon L Collinson
AIMS: To examine the diagnostic utility of the National Institute of Neurological Disorders and Stroke and the Canadian Stroke Network (NINDS-CSN) neuropsychological battery in memory clinics comparing controls with patients with no cognitive impairment (NCI), patients with cognitive impairment-no dementia (CIND) at varying severity levels (mild/moderate), and patients with dementia. METHODS: A total of 405 participants with NCI, CIND or dementia were assessed with the NINDS-CSN battery...
May 2016: Dementia and Geriatric Cognitive Disorders Extra
Fangyuan Zhang, Qunbi Zhuge, Meng Qiu, David V Plant
In this paper, we propose two modifications to reduce the complexity of the subcarrier-multiplexing (SCM) based digital backpropagation (DBP) for high symbol rate SCM systems. The first one is to reduce the number of interfering subcarriers (RS-SCM-DBP) when evaluating the cross-subcarrier nonlinearity (CSN). The second one is to replace the original frequency domain CSN filters with the infinite impulse response (IIR) filters (IIR-RS-SCM-DBP) in the CSN compensation. The performance of the proposed schemes are numerically evaluated in three-channel dual-polarization (DP) 16QAM wavelength-division multiplexing (WDM) transmissions...
July 25, 2016: Optics Express
Xiangliang Chen, Lihui Duan, Yunfei Han, Ling Tian, Qiliang Dai, Shang Wang, Ying Lin, Yunyun Xiong, Xinfeng Liu
BACKGROUND: Around two thirds stroke patients may suffer from vascular cognitive impairment (VCI). Our previous study has validated the NINDS-CSN harmonization standard for VCI diagnosis in Chinese. In this study, we aimed to investigate the predictors for VCI in Chinese post-stroke patients. METHODS: We compared epidemiological, clinical, and neuroimaging data (number, size and location of acute infarcts and lacunes, severities of white matter hyperintensities and brain atrophy) between stroke patients with and without VCI...
2016: BMC Neurology
Farzad Ashrafi, Behdad Behnam, Mehran Arab Ahmadi, Morteza Sanei Taheri, Hamid Reza Haghighatkhah, Hossein Pakdaman, Seyed Mohammad Hadi Kharrazi
BACKGROUND: Magnetic resonance imaging (MRI) has improved the diagnosis and management of patients with multiple sclerosis (MS). Montreal Cognitive Assessment (MoCA) is a brief, sensitive test that has been recommended by National Institute of Neurological Diseases and Stroke and Canadian Stroke Network (NINDS-CSN) as a reliable tool to detect mild cognitive impairments. This study aimed to evaluate the relationship between MoCA test and its sub-items with brain abnormalities in MRI of MS patients...
2016: Medical Journal of the Islamic Republic of Iran
E Pekary, A Sattin
Photoperiod-synchronized rhythms in non-CSN tissues persist in total darkness. Clock genes involved in maintaining regular biorhythms within the suprachiasmatic nucleus (SCN) of the hypothalamus are expressed in extra-CNS tissues and continue periodic expression in vitro. Understanding the details of how the SCN clock is coupled with peripheral clocks is only incompletely understood and may involve a multiplicity of feedback systems. The present study is an extension of our previous work showing that brain levels of TRH (pGlu-His-Pro-NH2) and TRH-like peptides (X-TRH: pGlu-X-Pro-NH2, where "X" can be any amino acid residue) fluctuate throughout the day-night cycle...
July 19, 2016: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
Feiran Zhang, Jia Fei, Minjie Sun, Qineng Ping
Polycations have been widely used as efficient drug and gene carriers. However, the further application of polycation nanocarriers is greatly hampered by the serious cytotoxicity caused by exposed positive charges. Despite recent progress towards the therapeutic delivery of nucleic acids, there remains a compelling need for development of novel delivery systems for various types of drug. Here, we created mixed micelles based on N-octyl-N-trimethyl chitosan (OTMC) and coated them with an anionic polymer for delivery of paclitaxel (PTX)...
September 10, 2016: International Journal of Pharmaceutics
T T Raij, T Mäntylä, O Mantere, T Kieseppä, J Suvisaari
BACKGROUND: Delusion is the most characteristic symptom of psychosis. While researchers suggested an association between changes of the cortical salience network (CSN) and delusion, whether these CSN findings are a cause or a consequence of delusion remains unknown. METHOD: To assess the effect of CSN functioning to forthcoming changes in delusion scores, we measured brain activation with 3-T functional magnetic resonance imaging in two independent samples of first-episode psychosis patients (total of 27 patients and 23 healthy controls)...
October 2016: Psychological Medicine
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"