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https://www.readbyqxmd.com/read/29332295/measurement-of-the-caspase-1-like-activity-of-vacuolar-processing-enzyme-in-plants
#1
Noriyuki Hatsugai, Ikuko Hara-Nishimura
Caspase-like activities are essential to regulate programed cell death in plants. Although no caspase orthologous enzymes with aspartic acid specificity have been identified in plants, vacuolar processing enzyme (VPE) exhibits a caspase-1-like activity. In this chapter, we introduce two methods for the measurement of the caspase-1-like/VPE activity. These methods are based on the cleavage of caspase-1 specific synthetic substrates and on monitoring the active forms of VPE using a biotinylated-inhibitor blot analysis...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29331460/inhibition-of-shigella-sonnei-induced-epithelial-barrier-disruption-by-surface-layer-associated-proteins-of-lactobacilli-from-chinese-fermented-food
#2
Yingchun Zhang, Xiaolu Shi, Siqi Hao, Qianhui Lu, Lanwei Zhang, Xue Han, Weihong Lu
Surface-layer associated proteins (SLAP) of Lactobacillus paracasei ssp. paracasei M5-L and Lactobacillus casei Q8-L were examined to identify the functional basis for their protection within intestinal epithelial cells. The results showed that SLAP of M5-L and Q8-L remained active in a trypsin solution and retained a 45-kDa protein band, similar to that observed in controls. In contrast, under conditions of simulated gastric juice, the SLAP were partially degraded. Inhibitory effects of SLAP on adherence of Shigella sonnei to HT-29 cells were assessed with use of exclusion, competition, and replacement assays...
January 10, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29331080/design-synthesis-and-evaluation-of-novel-oxazaborine-inhibitors-of-the-nlrp3-inflammasome
#3
Alex George Baldwin, Victor S Tapia, Tessa Swanton, Claire S White, James A Beswick, David Brough, Sally Freeman
The NLRP3 inflammasome is an important regulator of the sterile inflammatory response and its activation by host-derived sterile molecules leads to the intracellular activation of caspase-1, processing of the pro-inflammatory cytokines interleukin-1β (IL 1β)/IL-18, and pyroptotic cell death. Inappropriate activation of NLRP3 drives a chronic inflammatory response and is implicated in several non-communicable diseases, including gout, atherosclerosis, type II diabetes and Alzheimer's disease. In this study, we report the design, synthesis and biological evaluation of novel boron compounds (NBCs) as NLRP3 inflammasome inhibitors...
January 13, 2018: ChemMedChem
https://www.readbyqxmd.com/read/29330116/tnf-%C3%AE-increases-staphylococcus-aureus-induced-death-of-human-alveolar-epithelial-cell-line-a549-associated-with-rip3-mediated-necroptosis
#4
Shun-Hang Wen, Luo-Na Lin, Hu-Jun Wu, Lu Yu, Li Lin, Li-Li Zhu, Hai-Yan Li, Hai-Lin Zhang, Chang-Chong Li
AIM: To explore the role of tumor necrosis factor-alpha (TNF-α) on Staphylococcus aureus-induced necroptosis in alveolar epithelial cells. MAIN METHODS: The A549 alveolar epithelial cell line was pretreated with small interfering RNA (siRNA) against receptor interacting protein-3 (RIP3) and then stimulated by S. aureus, where some cells were pretreated with TNF-α or TNF-α with anti-TNF-α antibody simultaneously. A549 cell death was assessed using lactate dehydrogenase (LDH) leakage and flow cytometry analyses...
January 9, 2018: Life Sciences
https://www.readbyqxmd.com/read/29330013/a-functional-splicing-variant-associated-with-decreased-asthma-risk-abolishes-the-ability-of-gasdermin-b-gsmdb-to-induce-epithelial-cell-pyroptosis
#5
Ronald A Panganiban, Maoyun Sun, Amber Dahlin, Hae-Ryung Park, Mengyuan Kan, Blanca E Himes, Jennifer A Mitchel, Carlos Iribarren, Eric Jorgenson, Scott H Randell, Elliot Israel, Kelan Tantisira, Stephanie Shore, Jin-Ah Park, Scott T Weiss, Ann Chen Wu, Quan Lu
BACKGROUND: Genetic variants in the chromosomal region 17q21 are consistently associated with asthma. However, mechanistic studies have not yet linked any of the associated variants to a function that could influence asthma, and as a result, the identity of the asthma gene(s) remains elusive. OBJECTIVES: We sought to identify and characterize functional variants in the 17q21 locus. METHODS: We used the Exome Aggregation Consortium (ExAC) browser to identify coding (amino acid-changing) variants in the 17q21 locus...
January 9, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29329580/evidence-for-the-activation-of-pyroptotic-and-apoptotic-pathways-in-rpe-cells-associated-with-nlrp3-inflammasome-in-the-rodent-eye
#6
Jiangyuan Gao, Jing Z Cui, Eleanor To, Sijia Cao, Joanne A Matsubara
BACKGROUND: Age-related macular degeneration (AMD) is a devastating eye disease causing irreversible vision loss in the elderly. Retinal pigment epithelium (RPE), the primary cell type that is afflicted in AMD, undergoes programmed cell death in the late stages of the disease. However, the exact mechanisms for RPE degeneration in AMD are still unresolved. The prevailing theories consider that each cell death pathway works independently and without regulation of each other. Building upon our previous work in which we induced a short burst of inflammasome activity in vivo, we now investigate the effects of prolonged inflammasome activity on RPE cell death mechanisms in rats...
January 12, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29329289/burkholderia-pseudomallei-modulates-host-iron-homeostasis-to-facilitate-iron-availability-and-intracellular-survival
#7
Imke H E Schmidt, Claudia Gildhorn, Martha A L Böning, Vera A Kulow, Ivo Steinmetz, Antje Bast
BACKGROUND: The control over iron homeostasis is critical in host-pathogen-interaction. Iron plays not only multiple roles for bacterial growth and pathogenicity, but also for modulation of innate immune responses. Hepcidin is a key regulator of host iron metabolism triggering degradation of the iron exporter ferroportin. Although iron overload in humans is known to increase susceptibility to Burkholderia pseudomallei, it is unclear how the pathogen competes with the host for the metal during infection...
January 12, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29324327/elucidating-the-interfaces-involved-in-card-card-interactions-mediated-by-nlrp1-and-caspase-1-using-molecular-dynamics-simulation
#8
Jitendra Maharana
Inflammasomes are the multi-protein caspase-activating complexes mainly assembled by the sensor proteins (NLRs/ALRs), adaptor molecule (ASC) and effector molecule pro-caspase-1 for the production and release of proinflammatory cytokines, IL-1β and IL-18. NLRP1 is the first NLR known to assemble the multi-protein complex. Unlike NLRP3, NLRP1 has an additional effector binding domain (CARD) at the carboxyl-terminal, which is reported to interact with pro-caspase-1 (precluding the recruitment of ASC) for the transmission of danger signals...
December 24, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29323474/caspase-1-specific-light-up-probe-with-aggregation-induced-emission-characteristics-for-inhibitor-screening-of-coumarin-originated-natural-products
#9
Hao Lin, Haitao Yang, Shuai Huang, Fujia Wang, Dong-Mei Wang, Bin Liu, Yi-Da Tang, Chong-Jing Zhang
Caspase-1 is a key player in pyroptosis and inflammation. Caspase-1 inhibition is found to be beneficial to various diseases. Coumarin-originated natural products have an anti-inflammation function, but their direct inhibition effect to caspase-1 remains unexplored. To evaluate their interactions, the widely used commercial coumarin-based probe (Ac-YVAD-AMC) is not suitable, as the background signal from coumarin-originated natural products could interfere with the screening results. Therefore, fluorescent probes using a large Stokes shift could help solve this problem...
January 11, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29322421/immuno-detection-of-immature-and-bioactive-forms-of-the-inflammatory-cytokine-il-18
#10
Brendan J Jenkins, Virginie Deswaerte
Gastric cancer (GC) is the third most lethal cancer worldwide, and like many other types of cancers, it is associated with precursory chronic inflammatory responses. In the context of many inflammation-associated cancers such as GC, activation of the innate immune response by infectious microbes and/or host-derived molecules is often characterized by production of the cytokines interleukin (IL)-1β and IL-18, which can often have divergent and opposing (i.e., pro or anti) roles in inflammation and oncogenesis...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29322417/quantifying-caspase-1-activity-in-murine-macrophages
#11
Dave Boucher, Amy Chan, Connie Ross, Kate Schroder
The caspase-1 protease is a core component of multiprotein inflammasome complexes, which play a critical role in regulating the secretion of mature, bioactive pro-inflammatory cytokines interleukin (IL)-1β and IL-18. The activity of caspase-1 is often measured indirectly, by monitoring cleavage of cellular caspase-1 substrates, processing of caspase-1 itself, or by quantifying cell death. Here we describe methods for eliciting caspase-1 activity in murine macrophages, via activation of the NLRP3, NAIP/NLRC4 or AIM2 inflammasomes...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29321963/a-critical-role-of-hepatitis-b-virus-polymerase-in-cirrhosis-hepatocellular-carcinoma-and-steatosis
#12
Hea-Jong Chung, Xiao Chen, Yang Yu, Heui-Kwan Lee, Chang Ho Song, Han Choe, Seungkoo Lee, Hyeon-Jin Kim, Seong-Tshool Hong
Hepatitis B is one of the most common infectious diseases in the world; more than 350 million people are carriers of hepatitis B virus (HBV). Chronic HBV infection (CHB) leads to liver diseases such as cirrhosis, hepatocellular carcinoma (HCC), and steatosis. Despite its seriousness in terms of public health, the pathogenic mechanism of how CHB leads to liver diseases, especially cirrhosis and steatosis, remains unclear. We studied the role of HBV polymerase (HBp) reverse transcriptase (RT) activity in association with the pathogenesis of liver diseases in CHB by developing transgenic mice expressing HBp or the RT domain of HBp...
January 2018: FEBS Open Bio
https://www.readbyqxmd.com/read/29317641/zika-virus-infection-induces-host-inflammatory-responses-by-facilitating-nlrp3-inflammasome-assembly-and-interleukin-1%C3%AE-secretion
#13
Wenbiao Wang, Geng Li, De Wu, Zhen Luo, Pan Pan, Mingfu Tian, Yingchong Wang, Feng Xiao, Aixin Li, Kailang Wu, Xiaohong Liu, Lang Rao, Fang Liu, Yingle Liu, Jianguo Wu
Zika virus (ZIKV) infection is a public health emergency and host innate immunity is essential for the control of virus infection. The NLRP3 inflammasome plays a key role in host innate immune responses by activating caspase-1 to facilitate interleukin-1β (IL-1β) secretion. Here we report that ZIKV stimulates IL-1β secretion in infected patients, human PBMCs and macrophages, mice, and mice BMDCs. The knockdown of NLRP3 in cells and knockout of NLRP3 in mice inhibit ZIKV-mediated IL-1β secretion, indicating an essential role for NLRP3 in ZIKV-induced IL-1β activation...
January 9, 2018: Nature Communications
https://www.readbyqxmd.com/read/29317576/-protective-effect-of-taxifolin-on-h2o2-induced-%C3%A2-h9c2-cell-pyroptosis
#14
Yanqiong Ye, Xiaoli Wang, Qian Cai, Jian Zhuang, Xiaohua Tan, Wei He, Mingyi Zhao
To explore the effect of taxifolin on H2O2-induced pyroptosis in H9C2 cells and the possible mechanisms.
 Methods: The H9C2 cells was divided into 3 groups: a control group, a hydrogen peroxide (H2O2)group and a taxifolin group. The morphology of H9C2 cells was observed by inverted phase contrast microscope. The mitochondrial membrane potential was measured by JC-1 staining and flow cytometry. The alteration of the level of reactive oxygen species (ROS) was detected by specific mitochondrial probe. The protein levels of cysteinyl aspartate specific proteinase-1 (caspase-1)was determined by Western blot...
December 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/29314607/h3-relaxin-inhibits-the-collagen-synthesis-via-ros-and-p2x7r-mediated-nlrp3-inflammasome-activation-in-cardiac-fibroblasts-under-high-glucose
#15
Xiaohui Zhang, Yu Fu, Hui Li, Li Shen, Qing Chang, Liya Pan, Siting Hong, Xinhua Yin
Excessive production of reactive oxygen species (ROS) and P2X7R activation induced by high glucose increases NLRP3 inflammasome activation, which contributes to the pathogenesis of diabetic cardiomyopathy. Although H3 relaxin has been shown to inhibit cardiac fibrosis induced by isoproterenol, the mechanism has not been well studied. Here, we demonstrated that high glucose (HG) induced the collagen synthesis by activation of the NLRP3 inflammasome, leading to caspase-1 activation, interleukin-1β (IL-1β) and IL-18 secretion in neonatal rat cardiac fibroblasts...
January 5, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29313961/impaired-phagocytosis-directs-human-monocyte-activation-in-response-to-fungal-derived-%C3%AE-glucan-particles
#16
Giorgio Camilli, Elif Eren, David L Williams, Vishukumar Aimanianda, Etienne Meunier, Jessica Quintin
Recognition of the fungal cell wall carbohydrate β-glucan by the host receptor Dectin-1 elicits broad immunomodulatory responses, such as phagocytosis and activation of oxidative burst. These responses are essential for engulfing and killing fungal pathogens. Phagocytic monocytes are key mediators of these early host inflammatory responses to infection. Remarkably, whether phagocytosis of fungal β-glucan leads to an inflammatory response in human monocytes remains to be established. Here, we show that phagocytosis of heat-killed Candida albicans is essential to trigger inflammation and cytokine release...
January 5, 2018: European Journal of Immunology
https://www.readbyqxmd.com/read/29312598/interleukin-22-participates-in-the-inflammatory-process-of-vitiligo
#17
Jinjin Dong, Xiaohong An, Hui Zhong, Yichuan Wang, Jing Shang, Jia Zhou
Vitiligo is an acquired depigmentary skin inflammatory disorder. The pathogenesis of inflammatory skin disease involves the release of cytokines from keratinocytes, including interleukin (IL)-1β. IL-22 belongs to a family of cytokines structurally related to IL-10, including IL-19, IL-20, IL-24, and IL-26. In contrast to IL-10, IL-22 has proinflammatory activities. Among skin cell populations only keratinocytes are the major targets of IL-22. In the present study, we demonstrated that IL-22 promoting IL-1β secretion from keratinocytes via the Reactive oxygen species (ROS)-NOD-like receptor family, pyrin domain containing 3 (NLRP3)-caspase-1 pathway...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29304864/hypercapnia-induces-il-1%C3%AE-overproduction-via-activation-of-nlrp3-inflammasome-implication-in-cognitive-impairment-in-hypoxemic-adult-rats
#18
Hong-Guang Ding, Yi-Yu Deng, Ren-Qiang Yang, Qiao-Sheng Wang, Wen-Qiang Jiang, Yong-Li Han, Lin-Qiang Huang, Miao-Yun Wen, Wen-Hong Zhong, Xu-Sheng Li, Fan Yang, Hong-Ke Zeng
BACKGROUND: Cognitive impairment is one of common complications of acute respiratory distress syndrome (ARDS). Increasing evidence suggests that interleukin-1 beta (IL-1β) plays a role in inducing neuronal apoptosis in cognitive dysfunction. The lung protective ventilatory strategies, which serve to reduce pulmonary morbidity for ARDS patients, almost always lead to hypercapnia. Some studies have reported that hypercapnia contributes to the risk of cognitive impairment and IL-1β secretion outside the central nervous system (CNS)...
January 5, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29303910/involvement-of-nucleotide-binding-and-oligomerization-domain-like-receptors-in-the-intestinal-injury-of-severe-acute-pancreatitis-in-rats
#19
Shan Xu, Shuqing Wei, Yu Guo, Donglai Cui, Jinfeng Yao
OBJECTIVES: The aim of the study was to observe the role of nucleotide-binding and oligomerization domain (NOD)-like receptors (NLR) in intestine injury of severe acute pancreatitis (SAP) in rats. METHODS: Severe acute pancreatitis was induced by retrograde infusion of sodium taurocholate into the biliopancreatic duct. Rats were divided into the following 6 groups: sham operation, SAP treated with saline, and SAP treated with interleukin 1β (IL-1β)-converting enzyme inhibitor, killed at 6 or 12 hours after operation...
January 5, 2018: Pancreas
https://www.readbyqxmd.com/read/29303613/cellular-differentiation-of-non-transformed-intestinal-epithelial-cells-is-regulated-by-lactobacillus-rhamnosus-and-l-casei-strains
#20
J Kolinska, M Zakostelecka, Z Zemanova, V Lisa, J Golias, H Kozakova, B Dvorak
The aim of this study was to characterize an in vitro modulating effect of three commensal Lactobacillus strains on cellular differentiation of non-transformed crypt-like rat small intestinal cell line IEC-18. IEC-18 was grown on extracellular matrix (ECM), with or without presence of Lactobacillus strains. Gene expression of IEC-18 bacterial detection system - such as Toll-like receptors TLR-2, TLR-4, signal adapter MyD88, cytoplasmic NOD2 receptor, inflammatory cytokines IL-18, IL-1beta, chemokine IL-8 and enzyme caspase-1 - was evaluated using real-time PCR...
January 5, 2018: Physiological Research
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