keyword
https://read.qxmd.com/read/38452759/securin-acetylation-prevents-precocious-separase-activation-and-premature-sister-chromatid-separation
#1
JOURNAL ARTICLE
Tianning Wang, Yuhong Zou, Hui Meng, Pengli Zheng, Junlin Teng, Ning Huang, Jianguo Chen
Lysine acetylation of non-histone proteins plays crucial roles in many cellular processes. In this study, we examine the role of lysine acetylation during sister chromatid separation in mitosis. We investigate the acetylation of securin at K21 by cell-cycle-dependent acetylome analysis and uncover its role in separase-triggered chromosome segregation during mitosis. Prior to the onset of anaphase, the acetylated securin via TIP60 prevents its degradation by the APC/CCDC20 -mediated ubiquitin-proteasome system...
February 27, 2024: Current Biology: CB
https://read.qxmd.com/read/38445465/thermally-reversible-gel-sol-transition-of-hydrogels-via-dissociation-and-association-of-an-artificial-protein-nanocage
#2
JOURNAL ARTICLE
Erika Nasu, Norifumi Kawakami, Shuhei Takamura, Atsushi Hotta, Ryoichi Arai, Kenji Miyamoto
Oligomeric protein nanocages often disassemble into their subunits and reassemble by external stimuli. Thus, using these nanocages as cross-linkers for hydrogel network structures is a promising approach to allow hydrogels to undergo stimuli-responsive gel-sol transitions or self-healing. Here, we report hydrogels that show a reversible gel-sol transition resulting from the heat-induced dissociation and reassociation of protein nanocages. The hydrogel contained the 60-mer artificial protein nanocage, TIP60, as a supramolecular cross-linker for polyethylene glycol network structures...
March 6, 2024: Biomacromolecules
https://read.qxmd.com/read/38402617/an-iron-rheostat-controls-hematopoietic-stem-cell-fate
#3
JOURNAL ARTICLE
Yun-Ruei Kao, Jiahao Chen, Rajni Kumari, Anita Ng, Aliona Zintiridou, Madhuri Tatiparthy, Yuhong Ma, Maria M Aivalioti, Deeposree Moulik, Sriram Sundaravel, Daqian Sun, Julie A Reisz, Juliane Grimm, Nuria Martinez-Lopez, Stephanie Stransky, Simone Sidoli, Ulrich Steidl, Rajat Singh, Angelo D'Alessandro, Britta Will
Mechanisms governing the maintenance of blood-producing hematopoietic stem and multipotent progenitor cells (HSPCs) are incompletely understood, particularly those regulating fate, ensuring long-term maintenance, and preventing aging-associated stem cell dysfunction. We uncovered a role for transitory free cytoplasmic iron as a rheostat for adult stem cell fate control. We found that HSPCs harbor comparatively small amounts of free iron and show the activation of a conserved molecular response to limited iron-particularly during mitosis...
February 19, 2024: Cell Stem Cell
https://read.qxmd.com/read/38368419/spatiotemporal-and-direct-capturing-global-substrates-of-lysine-modifying-enzymes-in-living-cells
#4
JOURNAL ARTICLE
Hao Hu, Wei Hu, An-Di Guo, Linhui Zhai, Song Ma, Hui-Jun Nie, Bin-Shan Zhou, Tianxian Liu, Xinglong Jia, Xing Liu, Xuebiao Yao, Minjia Tan, Xiao-Hua Chen
Protein-modifying enzymes regulate the dynamics of myriad post-translational modification (PTM) substrates. Precise characterization of enzyme-substrate associations is essential for the molecular basis of cellular function and phenotype. Methods for direct capturing global substrates of protein-modifying enzymes in living cells are with many challenges, and yet largely unexplored. Here, we report a strategy to directly capture substrates of lysine-modifying enzymes via PTM-acceptor residue crosslinking in living cells, enabling global profiling of substrates of PTM-enzymes and validation of PTM-sites in a straightforward manner...
February 17, 2024: Nature Communications
https://read.qxmd.com/read/38352321/gene-specific-rna-homeostasis-revealed-by-perturbation-of-the-nua4-tip60-acetyltransferase-complex
#5
Faezeh Forouzanfar, Damien Plassard, Audrey Furst, David F Moreno, Karen A Oliveira, Bernardo Reina-San-Martin, László Tora, Nacho Molina, Manuel Mendoza
Transcript buffering entails the reciprocal modulation of mRNA synthesis and degradation rates, ensuring a constant RNA concentration amidst changes in cellular conditions. While an increasing body of research supports a global, non-sequence-specific linkage between mRNA synthesis and degradation, the underlying mechanisms remain elusive. To explore this, we investigated alterations in RNA metabolism following the acute depletion of TIP60/KAT5, the transcriptional coactivator and acetyltransferase subunit of the NuA4 complex, in mouse embryonic stem cells...
January 31, 2024: bioRxiv
https://read.qxmd.com/read/38260622/evidence-of-histone-h2a-z-deacetylation-and-cardiomyocyte-dedifferentiation-in-infarcted-tip60-depleted-hearts
#6
Xinrui Wang, Katherine Kulik, Tina C Wan, John W Lough, John A Auchampach
Myocardial infarction (MI) in the human heart causes death of billions of cardiomyocytes (CMs), resulting in cardiac dysfunction that is incompatible with life or lifestyle. In order to re-muscularize injured myocardium, re-placement CMs must be generated via renewed proliferation of surviving CMs. Approaches designed to induce proliferation of CMs after injury have been insufficient. Toward this end, we are targeting the Tip60 acetyltrans-ferase, based on the rationale that its pleiotropic functions conspire to block the CM cell-cycle at several check-points...
January 13, 2024: bioRxiv
https://read.qxmd.com/read/38250161/sgk1-aggravates-idiopathic-pulmonary-fibrosis-by-triggering-h3k27ac-mediated-macrophage-reprogramming-and-disturbing-immune-homeostasis
#7
JOURNAL ARTICLE
Jianzhi Wu, Liping Gong, Yijie Li, Tiegang Liu, Rong Sun, Kexin Jia, Runping Liu, Fei Dong, Xiaohong Gu, Xiaojiaoyang Li
Idiopathic pulmonary fibrosis (IPF) is characterized by fibrotic matrix deposition and irreversible aberrant tissue remodeling. Their mechanisms of action are associated with the activation of macrophages and a disturbed immune environment. We aim to determine how these activated macrophages influenced the pathogenesis of pulmonary fibrosis. We found the fibrotic areas of IPF patients contained more serum and glucocorticoid-induced kinase 1 (SGK1)-positive and M2-type macrophages. Similarly, bleomycin (BLM)+LPS significantly triggered high expression of SGK1 in the IPF mice, accompanied by destroyed lung structure and function, increased fibrosis markers and disturbed immune microenvironment...
2024: International Journal of Biological Sciences
https://read.qxmd.com/read/38242344/cebpa-facilitates-loxl2-and-loxl3-transcription-to-promote-bcl-2-stability-and-thus-enhances-the-growth-and-metastasis-of-lung-carcinoma-cells-in-vitro
#8
JOURNAL ARTICLE
Lihua Fan, Weijuan Jiang, Canjuan Chen, Hong Gao, Jiangwei Shi, Dewen Wang
Lung carcinoma (LC) is a complicated and highly heterogeneous disease with high morbidity and mortality. Both lysyl oxidase-like (LOXL) 2 and 3 act in cancer progression. This work endeavors to illustrate the influence of LOXL2/LOXL3 on LC progression and the underlying mechanisms. LOXL family genes and CCAAT enhancer binding protein A (CEBPA) were analyzed in the TCGA database for their expression patterns in LC patients and their correlations with the patient's prognosis. CEBPA, LOXL2, and LOXL3 expression levels were determined in LC cells...
January 17, 2024: Experimental Cell Research
https://read.qxmd.com/read/38184505/combination-of-resveratrol-and-parp-inhibitor-olaparib-efficiently-deregulates-homologous-recombination-repair-pathway-in-breast-cancer-cells-through-inhibition-of-tip60-mediated-chromatin-relaxation
#9
JOURNAL ARTICLE
Saptarshi Sinha, Subarno Paul, Sushree Subhadra Acharya, Chinmay Das, Somya Ranjan Dash, Subhasmita Bhal, Rajalaxmi Pradhan, Biswajit Das, Chanakya Nath Kundu
Recently, we reported that a combination of a natural, bioactive compound Resveratrol (RES) and a PARP inhibitor Olaparib (OLA) deregulated the homologous recombination (HR) pathway, and enhanced apoptosis in BRCA1-wild-type, HR-proficient breast cancer cells. Upon DNA damage, chromatin relaxation takes place, which allows the DNA repair proteins to access the DNA lesion. But whether chromatin remodeling has any role in RES + OLA-mediated HR inhibition is not known. By using in vitro and ex vivo model systems of breast cancer, we have investigated whether RES + OLA inhibits chromatin relaxation and thereby blocks the HR pathway...
January 6, 2024: Medical Oncology
https://read.qxmd.com/read/38141874/the-tudor-knot-domain-of-kat5-regulates-nucleosomal-substrate-acetylation
#10
JOURNAL ARTICLE
Fan Xuan, Hongwen Xuan, Mengying Huang, He Wei, Han Xu, Xiaobing Shi, Hong Wen
The lysine acetyltransferase KAT5 is a pivotal enzyme responsible for catalyzing histone H4 acetylation in cells. In addition to its indispensable HAT domain, KAT5 also encompasses a conserved Tudor-knot domain at its N-terminus. However, the function of this domain remains elusive, with conflicting findings regarding its role as a histone reader. In our study, we have employed a CRISPR tiling array approach and unveiled the Tudor-knot motif as an essential domain for cell survival. The Tudor-knot domain does not bind to histone tails and is not required for KAT5's chromatin occupancy...
December 21, 2023: Journal of Molecular Biology
https://read.qxmd.com/read/38094248/small-molecule-tip60-inhibitors-enhance-regulatory-t%C3%A2-cell-induction-through-tip60-p300-acetylation-crosstalk
#11
JOURNAL ARTICLE
Francisco Fueyo-González, Guillermo Vilanova, Mehek Ningoo, Nada Marjanovic, Juan A González-Vera, Ángel Orte, Miguel Fribourg
Foxp3 acetylation is essential to regulatory T (Treg) cell stability and function, but pharmacologically increasing it remains an unmet challenge. Here, we report that small-molecule compounds that inhibit TIP60, an acetyltransferase known to acetylate Foxp3, unexpectedly increase Foxp3 acetylation and Treg induction. Utilizing a dual experimental/computational approach combined with a newly developed FRET-based methodology compatible with flow cytometry to measure Foxp3 acetylation, we unraveled the mechanism of action of these small-molecule compounds in murine and human Treg induction cell cultures...
December 15, 2023: IScience
https://read.qxmd.com/read/38076914/h2a-z-chaperones-converge-on-histone-h4-acetylation-for-melanoma-cell-proliferation
#12
Sina Jostes, Chiara Vardabasso, Joanna Dong, Saul Carcamo, Rajendra Singh, Robert Phelps, Austin Meadows, Dan Hasson, Emily Bernstein
High levels of H2A.Z promote melanoma cell proliferation and correlate with poor prognosis. However, the role of the two distinct H2A.Z histone chaperone complexes, SRCAP and P400-TIP60, in melanoma remains unclear. Here, we show that individual depletion of SRCAP , P400 , and VPS72 (YL1) not only results in loss of H2A.Z deposition into chromatin, but also a striking reduction of H4 acetylation in melanoma cells. This loss of H4 acetylation is found at the promoters of cell cycle genes directly bound by H2A...
November 27, 2023: bioRxiv
https://read.qxmd.com/read/38062102/ep400-tip60-compensates-for-swi-snf-loss
#13
JOURNAL ARTICLE
Tiago Faial
No abstract text is available yet for this article.
December 2023: Nature Genetics
https://read.qxmd.com/read/38042310/bap18-acting-as-a-novel-peroxisome-proliferator-activated-receptor-%C3%AE-co-regulator-contributes-to-hepatocellular-carcinoma-progression
#14
JOURNAL ARTICLE
Wei Liu, Shengli Wang, Lin Lin, Renlong Zou, Hongmiao Sun, Kai Zeng, Yi Wu, Yiling Li, Kato Shigeaki, Xiuxia Wang, Chunyu Wang, Yue Zhao
Hepatocellular carcinoma (HCC) is a common malignancy worldwide with a poor prognosis. The therapeutic outcomes of HCC patients are urgently needed to be improved, and predictive biomarkers for the optimal treatment selection remains to be further defined. In the present study, our results showed that BPTF-associated protein of 18 KDa (BAP18) was highly expressed in HCC tissues. In cultured HCC cells, BAP18 regulated a subset of down-stream genes involved in different functions, particularly including peroxisome proliferator-activated receptor (PPAR) pathway and lipid metabolism...
November 30, 2023: Biochimica et Biophysica Acta. Molecular Basis of Disease
https://read.qxmd.com/read/38029912/myst-family-histone-acetyltransferases-regulate-reproductive-diapause-initiation
#15
JOURNAL ARTICLE
Hao-Min An, Yi-Fei Dai, Jun Zhu, Wen Liu, Xiao-Ping Wang
Histone acetylation, a crucial epigenetic mechanism, has been suggested to play a role in diapause regulation, but this has not been confirmed through gene loss-of-function studies. In this work, we investigated the involvement of MYST family genes, which are key writers of histone acetylation, in initiating reproductive diapause using the cabbage beetle Colaphellus bowringi as a model. We identified C. bowringi orthologs of MYST, including Tip60, KAT6A, KAT7, and KAT8, from previous transcriptomes. Analyses of phylogenetic trees and protein domains indicated that these MYST proteins are structurally conserved across animal species...
November 27, 2023: International Journal of Biological Macromolecules
https://read.qxmd.com/read/37982870/unconventional-roles-of-chromatin-remodelers-and-long-non-coding-rnas-in-cell-division
#16
REVIEW
Yuri Prozzillo, Maria Virginia Santopietro, Giovanni Messina, Patrizio Dimitri
The aim of this review article is to focus on the unconventional roles of epigenetic players (chromatin remodelers and long non-coding RNAs) in cell division, beyond their well-characterized functions in chromatin regulation during cell differentiation and development. In the last two  decades, diverse experimental evidence has shown that subunits of SRCAP and p400/TIP60 chromatin remodeling complexes in humans relocate from interphase nuclei to centrosomes, spindle or midbody, with their depletion yielding an array of aberrant outcomes of mitosis and cytokinesis...
November 20, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/37922899/global-identification-of-swi-snf-targets-reveals-compensation-by-ep400
#17
JOURNAL ARTICLE
Benjamin J E Martin, Eileen F Ablondi, Christine Goglia, Claudia A Mimoso, Piero R Espinel-Cabrera, Karen Adelman
Mammalian SWI/SNF chromatin remodeling complexes move and evict nucleosomes at gene promoters and enhancers to modulate DNA access. Although SWI/SNF subunits are commonly mutated in disease, therapeutic options are limited by our inability to predict SWI/SNF gene targets and conflicting studies on functional significance. Here, we leverage a fast-acting inhibitor of SWI/SNF remodeling to elucidate direct targets and effects of SWI/SNF. Blocking SWI/SNF activity causes a rapid and global loss of chromatin accessibility and transcription...
November 22, 2023: Cell
https://read.qxmd.com/read/37817460/tip60-mediates-stress-induced-hypertension-via-promoting-glutamate-dmpfc-to-vca1-release
#18
JOURNAL ARTICLE
Ying Wang, Min Xia, Jincheng Lu, Tianyu Wang, Xuan Zhang, Michael Ntim, Bin Wang
Hypertension is well-known to be influenced by genetic and environmental factors. Managing stress is one of the non-pharmacologic approaches to treating hypertension. It is, therefore, imperative to unravel the molecular mechanism by which stress conditions influence hypertension. In this study, TIP60 expressions in human blood samples and cell lines, glutamatedmPFC- to - vCA1 release, and receptor expressions in the Stress-induced hypertension mice were determined using western blotting, CSF (obtained by microdialysis), and ELISA...
December 31, 2023: Clinical and Experimental Hypertension: CHE
https://read.qxmd.com/read/37793204/3-3-diindolylmethane-a-natural-aryl-hydrocarbon-receptor-agonist-alleviates-ulcerative-colitis-by-enhancing-glycolysis-lactate-stat3%C3%A2-and-tip60-signals-mediated-treg-differentiation
#19
JOURNAL ARTICLE
Shukun Liu, Wenxin Yan, Qi Lv, Ling Yang, Yumeng Miao, Yuxiao Hu, Zhifeng Wei
BACKGROUND: Aryl hydrocarbon receptor (AhR) plays an important role in the occurrence and development of ulcerative colitis (UC). In this study, the effect and mechanism of 3, 3'-diindolylmethane (DIM), the classical AhR agonist, on UC was investigated from the angle of recovering the balance of Th17/Treg. METHODS: The in vivo colitis model was established in mice by using dextran sulfate sodium, and CD4+ T cells were used to simulate the in vitro differentiation of Treg and Th17 cells...
October 2, 2023: Molecular Immunology
https://read.qxmd.com/read/37720433/circrhot1-restricts-gastric-cancer-cell-ferroptosis-by-epigenetically-regulating-gpx4
#20
JOURNAL ARTICLE
Huan Wang, Daniel Adam Breadner, Ke Deng, Jing Niu
BACKGROUND: Gastric cancer (GC) is a malignant form of cancer that severely threatens human health. Despite developments on treatment, the prognosis of patients with advanced GC remains poor. Hence, the identification of detailed molecular mechanisms and potential therapeutic targets is of great importance for GC study. In recent years, circular RNAs have been widely reported to be important regulators in cancer initiation and progression. This study sought to evaluate the function of circRHOT1 in GC development...
August 31, 2023: Journal of Gastrointestinal Oncology
keyword
keyword
67899
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.