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https://www.readbyqxmd.com/read/28433859/photoelectrochemical-immunosensor-for-methylated-rna-detection-based-on-g-c3n4-cds-quantum-dots-heterojunction-and-phos-tag-biotin
#1
Haiyan Wang, Qihai Zhang, Huanshun Yin, Minghui Wang, Wenjing Jiang, Shiyun Ai
N(6)-methyladenosine (m(6)A) is an enigmatic and abundant internal modification in eukaryotic messenger RNA (mRNA), which could affect various aspects of RNA metabolism and mRNA translation. Herein, a novel photoelectrochemical (PEC) immunosensor was constructed for m(6)A detection based on the inhibition of Cu(2+) to the photoactivity of g-C3N4/CdS quantum dots (g-C3N4/CdS) heterojunction, where g-C3N4/CdS heterojunction was used as photoactive material, anti-m(6)A antibody as recognition unit for m(6)A-containing RNA, Phos-tag-biotin as link unit and avidin functionalized CuO as PEC signal indicator...
April 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28432639/measuring-patient-experience-in-dialysis-a-new-paradigm-of-quality-assessment
#2
REVIEW
Connie M Rhee, Steven M Brunelli, Lalita Subramanian, Francesca Tentori
Patients' experience of care (PEC) is as an important dimension in quality of care. As a distinct entity from patient satisfaction and patient health-related quality of life, PEC is defined as patients' perceptions of the range of interactions they have with the health care system, including care from providers, facilities, and health plans. While traditionally PEC may be ascertained via informal assessments, in recent years, especially in the United States, there has been a shift towards standardized surveillance of PEC amongst dialysis patients in order to: (1) set a normative expectation regarding the importance of PEC; (2) standardize the components of patients' experience that are assessed to minimize potential "blind spots"; (3) provide a direct "voice" to the patient in communicating perceptions of their care; (4) facilitate comparisons of quality across facilities; and (5) broaden accountability for PEC to the entire multidisciplinary dialysis care team...
April 21, 2017: Journal of Nephrology
https://www.readbyqxmd.com/read/28429812/hollow-hematite-single-crystals-deposited-with-ultra-thin-al2o3-by-atom-layer-deposition-for-improved-photoelectrochemical-performance
#3
Wei Jiao, Jingrui Wu, Siwen Cui, Ning Wei, Zia Ur Rahman, Meiyan Yu, Shougang Chen, Yangtao Zhou, Daoai Wang
Hematite (α-Fe2O3) is a red material with a band gap of about 2.0 eV, which indicates that it can absorb more solar light. It is a promising photocatalyst applied in many fields. In this paper, α-Fe2O3 single crystal hollow hexagonal bipyramids were synthesized by a simple one-pot hydrothermal method. The morphology and structure of the prepared α-Fe2O3 hollow hexagonal bipyramids were studied using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The hollow single crystals show a good light absorption and performance in photodegradation of methylene blue (MB)...
April 21, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28419503/comparison-of-the-quantitative-dry-culture-methods-with-both-conventional-media-and-most-probable-number-method-for-the-enumeration-of-coliforms-and-escherichia-coli-coliforms-in-food
#4
Hajime Teramura, Kojiro Sota, Mihoko Iwasaki, Hirokazu Ogihara
Sanita-kun(™) CC (coliform count) and EC (Escherichia coli/coliform count), sheet quantitative culture systems which can avoid chromogenic interference by lactase in food, were evaluated in comparison with conventional methods for these bacteria. Based on the results of inclusivity and exclusivity studies using 77 microorganisms, sensitivity and specificity of both Sanita-kun(™) met the criteria for ISO 16140. Both media were compared with deoxycholate agar, violet red bile agar, Merck Chromocult(™) coliform agar (CCA), 3M Petrifilm(™) CC and EC (PEC), and 3-tube MPN, as reference methods, in 100 naturally contaminated food samples...
April 17, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28412658/cds-mn-quantum-dot-functionalized-g-c3n4-nanohybrids-as-signal-generation-tags-for-photoelectrochemical-immunoassay-of-prostate-specific-antigen-coupling-dnazyme-concatamer-with-enzymatic-biocatalytic-precipitation
#5
Kangyao Zhang, Shuzhen Lv, Zhenzhen Lin, Dianping Tang
A new photoelectrochemical (PEC) immunosensor based on Mn-doped CdS quantum dots (CdS:Mn QDs) on g-C3N4 nanosheets was developed for the sensitive detection of prostate specific antibody (PSA) in biological fluids. The signal derived from the as-synthesized Cd:Mn QDs-functionalized g-C3N4 nanohybrids via a hydrothermal method and was amplified through DNAzyme concatamers on gold nanoparticles accompanying enzymatic biocatalytic precipitation. Experimental results by UV-vis absorption spectra and photoluminescence revealed that CdS:Mn QDs/g-C3N4 nanohybrids exhibited higher photocurrent than those of CdS:Mn QDs and g-C3N4 alone...
April 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28412354/role-of-gpr30-in-estrogen-induced-prostate-epithelial-apoptosis-and-benign-prostatic-hyperplasia
#6
Deng-Liang Yang, Jia-Wen Xu, Jianguo Zhu, Yi-Lin Zhang, Jian-Bang Xu, Qing Sun, Xiao-Nian Cao, Wu-Lin Zuo, Ruo-Shui Xu, Jiehong Huang, Fu-Neng Jiang, Yang-Jia Zhuo, Bai-Quan Xiao, Yun-Zhong Liu, Dongbo Yuan, Zhaolin Sun, Hui-Chan He, Zhao-Rong Lun, Wei-De Zhong, Wen-Liang Zhou
Several studies have implicated estrogen and the estrogen receptor (ER) in the pathogenesis of benign prostatic hyperplasia (BPH); however, the mechanism underlying this effect remains elusive. In the present study, we demonstrated that estrogen (17β-estradiol, or E2)-induced activation of the G protein-coupled receptor 30 (GPR30) triggered Ca(2+) release from the endoplasmic reticulum, increased the mitochondrial Ca(2+) concentration, and thus induced prostate epithelial cell (PEC) apoptosis. Both E2 and the GPR30-specific agonist G1 induced a transient intracellular Ca(2+) release in PECs via the phospholipase C (PLC)-inositol 1, 4, 5-triphosphate (IP3) pathway, and this was abolished by treatment with the GPR30 antagonist G15...
April 12, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28411647/nanoarmored-enzymes-for-organic-enzymology-synthesis-and-characterization-of-poly-2-alkyloxazoline-enzyme-conjugates
#7
Melanie Leurs, Joerg C Tiller
The properties of enzymes can be altered significantly by modification with polymers. Numerous different methods are known to obtain such polymer-enzyme conjugates (PECs). However, there is no universal method to render enzymes into PECs that are fully soluble in organic solvents. Here, we present a method, which achieves such high degree of modification of proteins that the majority of modified enzymes will be soluble in organic solvents. This is achieved by preparing poly(2-alkyloxazoline)s (POx) with an NH2 end group and coupling this functional polymer via pyromellitic acid dianhydride onto the amino groups of the respective protein...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28409636/signal-on-photoelectrochemical-immunoassay-for-aflatoxin-b1-based-on-enzymatic-product-etching-mno2-nanosheets-for-dissociation-of-carbon-dots
#8
Youxiu Lin, Qian Zhou, Dianping Tang, Reinhard Niessner, Dietmar Knopp
Aflatoxin B1 (AFB1) monitoring has attracted extensive attention because food safety is a worldwide public health problem. Herein, we design a novel simultaneously visual and photoelectrochemical (PEC) immunosensing system for rapid sensitive detection of AFB1 in foodstuff. The immunoreaction was carried out on anti-AFB1 antibody-modified magnetic beads by using glucose oxidase (GOx)-labeled AFB1-bovine serum albumin (AFB1-BSA) conjugates as the tags with a competitive-type immunoassay format, while the visual and PEC evaluation was performed via carbon quantum dots (CQDs)-functionalized MnO2 nanosheets...
April 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28401236/an-unconventional-outer-to-inner-synthesis-strategy-for-core-au-shell-nanostructures-with-photo-electrochemical-enhancement
#9
Zhuo Zhang, Minki Baek, Hongseon Song, Kijung Yong
In this work, an outer-to-inner strategy is demonstrated to simultaneously fabricate core-shell NPs and assemble them onto a scaffold. Specifically, the shell material is deposited onto the scaffold first, and then a layer of the core material (Au) is covered on the shell surface. Finally, the core (Au)-shell nanoparticles (NPs) are formed on the scaffold after annealing. As examples, Au-Bi2S3, Au-CdS and Au-CdSe core-shell NPs are grown on the surface of ZnO nanorods (NRs) via this strategy and exhibit enhanced photoelectrochemical (PEC) efficiency...
April 20, 2017: Nanoscale
https://www.readbyqxmd.com/read/28399702/early-flare-up-of-severe-herpes-gestationis-pemphigoid-gestationis-and-successfull-prolonged-treatment-a-case-report
#10
Pavol Zubor, Iveta Svecova, Petra Moricova, Juraj Pec, Katarina Adamicova, Jan Danko
No abstract text is available yet for this article.
April 11, 2017: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28397882/photoanodes-based-on-tio2-and-%C3%AE-fe2o3-for-solar-water-splitting-superior-role-of-1d-nanoarchitectures-and-of-combined-heterostructures
#11
REVIEW
Stepan Kment, Francesca Riboni, Sarka Pausova, Lei Wang, Lingyun Wang, Hyungkyu Han, Zdenek Hubicka, Josef Krysa, Patrik Schmuki, Radek Zboril
Solar driven photoelectrochemical water splitting (PEC-WS) using semiconductor photoelectrodes represents a promising approach for a sustainable and environmentally friendly production of renewable energy vectors and fuel sources, such as dihydrogen (H2). In this context, titanium dioxide (TiO2) and iron oxide (hematite, α-Fe2O3) are among the most investigated candidates as photoanode materials, mainly owing to their resistance to photocorrosion, non-toxicity, natural abundance, and low production cost. Major drawbacks are, however, an inherently low electrical conductivity and a limited hole diffusion length that significantly affect the performance of TiO2 and α-Fe2O3 in PEC devices...
April 11, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28391866/nano-encapsulant-of-ascorbic-acid-loaded-apoferritin-assisted-photoelectrochemical-sensor-for-protease-detection
#12
Jiexia Chen, Guang-Chao Zhao
A signal-on photoelectrochemical (PEC) biosensor based on nano-encapsulant of ascorbic acid-loaded apoferritin-assisted for protease detection is described. The loaded ascorbic acid could be released from the central cavity of apoferritin into the buffer solution as sacrificial electron donor to capture the photo-generated holes of CdTe quantum dots when light is turned on. In this system, the biosensor relied on monitoring the photocurrent intensity as a result of enzymatic substrate proteolysis in the homogeneous aqueous solution...
June 1, 2017: Talanta
https://www.readbyqxmd.com/read/28391680/stoichiometry-morphology-and-size-controlled-electrochemical-fabrication-of-cuxo-x-1-2-at-underpotential
#13
Cemile Kartal, Yeşim Hanedar, Tuba Öznülüer, Ümit Demir
A new one-step electrochemical approach has been developed for the morphology, size, and stoichiometry-controlled synthesis of Cu2O, CuO, and Cu2O/CuO composite structures at room temperature without using surfactants, capping agents, or any other additives. The electrochemical deposition of a Cu monolayer using underpotential deposition (UPD) and the flow rate of oxygen gas bubbled through the deposition solution used for oxidation of the Cu layer are the key parameters for controlling the stoichiometry of the CuxO (x = 1, 2) structures...
April 14, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28391638/construction%C3%A2-of-er-3-yalo3-rgo-tio2-hybrid-electrode-with%C3%A2-enhanced%C3%A2-photoelectrocatalytic%C3%A2-performance%C3%A2-in-methylene%C3%A2-blue%C3%A2-degradation%C3%A2-under%C3%A2-visible%C3%A2-light
#14
Xian Zhou, Jun Zhang, Yue Ma, Hanqing Cheng, Shaozhu Fu, Dandan Zhou, Shuangshi Dong
Much attention has been paid on doping TiO2 to narrow its band gap to promote the absorption of visible light and restrain the recombination of electron-hole pairs to improve its efficiency in photoelectrocatalysis (PEC) under visible light irradiation. However, the oxidation potential energy of photo-induced holes for the modified catalysts by visible light excitation is lower than that without modification by UV excitation. In this work, we synthesized a co-coupled TiO2 electrode (denoted ERT) with the Er(3+) :YAlO3 and reduced graphene oxide (RGO), achieving the synergetic effect of visible-light-to-UV up conversion and response and great electron transfer ability...
April 9, 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28389396/robust-identification-of-metabolic-control-for-microbial-l-methionine-production-following-an-easy-to-use-puristic-approach
#15
A Teleki, M Rahnert, O Bungart, B Gann, I Ochrombel, R Takors
The identification of promising metabolic engineering targets is a key issue in metabolic control analysis (MCA). Conventional approaches make intensive use of model-based studies, such as exploiting post-pulse metabolic dynamics after proper perturbation of the microbial system. Here, we present an easy-to-use, purely data-driven approach, defining pool efflux capacities (PEC) for identifying reactions that exert the highest flux control in linear pathways. Comparisons with linlog-based MCA and data-driven substrate elasticities (DDSE) showed that similar key control steps were identified using PEC...
April 4, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28387390/a-potentiometric-resolved-ratiometric-photoelectrochemical-aptasensor
#16
Ying Zhang, Nan Hao, Zhou Zhou, Rong Hua, Jing Qian, Qian Liu, Henan Li, Kun Wang
A novel ratiometric photoelectrochemical aptasensor based on the potentiometric resolved photocurrents generated from different PEC active materials was designed for the first time.
April 7, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28384408/polymer-dots-for-photoelectrochemical-bioanalysis
#17
Yu Li, Nan Zhang, Wei-Wei Zhao, De-Chen Jiang, Jing-Juan Xu, Hong-Yuan Chen
Different from the most extensively used inorganic quantum dots (Qdots) for the current state-of-the-art photoelectrochemical (PEC) bioanalysis, this work reports the first demonstration of polymer dots (Pdots) for novel PEC bioanalysis. The semiconducting Pdots were prepared via the reprecipitation method and then immobilized onto the transparent indium tin oxide glass electrode for PEC biodetection of the model molecule l-cysteine. The experimental results revealed that the as-fabricated Pdots exhibited excellent and interesting PEC activity and good analytical performance of rapid response, high stability, wide linear range, and excellent selectivity...
April 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28384395/consensus-bundle-on-maternal-mental-health-perinatal-depression-and-anxiety
#18
Susan Kendig, John P Keats, M Camille Hoffman, Lisa B Kay, Emily S Miller, Tiffany A Moore Simas, Ariela Frieder, Barbara Hackley, Pec Indman, Christena Raines, Kisha Semenuk, Katherine L Wisner, Lauren A Lemieux
Perinatal mood and anxiety disorders are among the most common mental health conditions encountered by women of reproductive age. When left untreated, perinatal mood and anxiety disorders can have profound adverse effects on women and their children, ranging from increased risk of poor adherence to medical care, exacerbation of medical conditions, loss of interpersonal and financial resources, smoking and substance use, suicide, and infanticide. Perinatal mood and anxiety disorders are associated with increased risks of maternal and infant mortality and morbidity and are recognized as a significant patient safety issue...
March 2017: Journal of Midwifery & Women's Health
https://www.readbyqxmd.com/read/28380361/efficient-generation-of-glucose-responsive-beta-cells-from-isolated-gp2-human-pancreatic-progenitors
#19
Jacqueline Ameri, Rehannah Borup, Christy Prawiro, Cyrille Ramond, Karen A Schachter, Raphael Scharfmann, Henrik Semb
Stem cell-based therapy for type 1 diabetes would benefit from implementation of a cell purification step at the pancreatic endoderm stage. This would increase the safety of the final cell product, allow the establishment of an intermediate-stage stem cell bank, and provide a means for upscaling β cell manufacturing. Comparative gene expression analysis revealed glycoprotein 2 (GP2) as a specific cell surface marker for isolating pancreatic endoderm cells (PECs) from differentiated hESCs and human fetal pancreas...
April 4, 2017: Cell Reports
https://www.readbyqxmd.com/read/28376618/cage-confinement-pyrolysis-route-to-ultrasmall-tungsten-carbide-nanoparticles-for-efficient-electrocatalytic-hydrogen-evolution
#20
Yan-Tong Xu, Xiaofen Xiao, Zi-Ming Ye, Shenlong Zhao, Rongan Shen, Chun-Ting He, Jie-Peng Zhang, Yadong Li, Xiao-Ming Chen
The size-controlled synthesis of ultrasmall metal-based catalysts is of vital importance for chemical conversion technologies. Here, a cage-confinement pyrolysis strategy is presented for the synthesis of ultrasmall tungsten carbide nanoclusters/nanoparticles. An RHO type zeolitic metal azolate framework MAF-6, possessing large nanocages and small apertures, is selected to confine the metal source W(CO)6. High temperature pyrolysis gives tungsten carbide nanoclusters/nanoparticles with sizes ca. 2 nm, which can serve as an excellent electrocatalyst for the hydrogen evolution reaction...
April 10, 2017: Journal of the American Chemical Society
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