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https://www.readbyqxmd.com/read/28613057/-analysis-of-factors-related-to-the-number-of-mesenchymal-stem-cells-derived-from-synovial-fluid-of-the-temporomandibular-joint
#1
Y P Sun, Y H Zheng, Z G Zhang
Objective: To analyze related factors on the number of mesenchymal stem cells in the synovial fluid of the temporomandibular joint (TMJ) and provide an research basis for understanding of the source and biological role of mesenchymal stem cells derived from synovial fluid in TMJ. Methods: One hundred and twenty-two synovial fluid samples from 91 temporomandibular disorders (TMD) patients who visited in Department of TMJ Center, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University from March 2013 to December 2013 were collected in this study, and 6 TMJ synovial fluid samples from 6 normal volunteers who were studying in the North Campus of Sun Yat-sen University were also collected, so did their clinical information...
June 9, 2017: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/28606250/-three-dimensional-finite-element-model-of-a-modified-posterior-cervical-single-open-door-laminoplasty
#2
Q Wang, Y Yang, Q Fei, D Li, J J Li, H Meng, N Su, Z H Fan, B Q Wang
Objective: To build a three-dimensional finite element models of a modified posterior cervical single open-door laminoplasty with short-segmental lateral mass screws fusion. Methods: The C(2)-C(7) segmental data were obtained from computed tomography (CT) scans of a male patient with cervical spondylotic myelopathy and spinal stenosis.Three-dimensional finite element models of a modified cervical single open-door laminoplasty (before and after surgery) were constructed by the combination of software package MIMICS, Geomagic and ABAQUS...
June 6, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28584817/molecular-cloning-expression-profiling-and-marker-validation-of-the-chicken-myoz3-gene
#3
Maosen Ye, Fei Ye, Liutao He, Yiping Liu, Xiaoling Zhao, Huadong Yin, Diyan Li, Hengyong Xu, Qing Zhu, Yan Wang
Myozenin3 (Myoz3) has been reported to bind multiple Z-disc proteins and hence play a key role in signal transduction and muscle fiber type differentiation. The purpose of current study is to better understand the basic characteristics of Myoz3. Firstly, we cloned the ORF (open reading frame) of the Myoz3 gene. AA (amino acid) sequence analysis revealed that the Myoz3 gene encodes a 26 kDa protein which have 97% identities with that of turkey. Expression profiling showed that Myoz3 mRNA is mainly expressed in leg muscle and breast muscle...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28547665/comparison-of-endoscopic-ultrasonography-and-magnifying-endoscopy-for-assessment-of-the-invasion-depth-of-shallow-gastrointestinal-neoplasms-a-systematic-review-and-meta-analysis
#4
Zhang Tao, Chen Yan, He Zhao, Jiawei Tsauo, Xiaowu Zhang, Bing Qiu, Yanqing Zhao, Xiao Li
OBJECTIVES: To conduct a meta-analysis to provide accurate evidence regarding the preferred diagnostic method, magnifying endoscopy (ME) or endoscopic ultrasonography (EUS), for assessment of the depth of invasion of the gastrointestinal neoplasms. METHODS: PubMed, EMBASE, Ovid Medline, and the Cochrane Library databases were searched for studies published between January 1946 and October 2016, regarding the use of EUS and ME to assess the invasion depth of gastrointestinal cancers...
May 25, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28543538/clinical-and-histopathological-features-of-myofibrillar-myopathy-in-warmblood-horses
#5
S J Valberg, A M Nicholson, S S Lewis, R A Reardon, C J Finno
BACKGROUND: To report a novel exertional myopathy, myofibrillar myopathy (MFM), in Warmblood horses. OBJECTIVES: To 1) describe the distinctive clinical and myopathic features of MFM in Warmblood horses, 2) investigate the potential inheritance of MFM in a Warmblood family. STUDY DESIGN: Retrospective selection of MFM cases and prospective evaluation of a Warmblood family. METHODS: Retrospectively, muscle biopsies were selected from Warmblood horses diagnosed with MFM and clinical histories obtained (n = 10)...
May 22, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/28542873/a-map-of-the-phosphoproteomic-alterations-that-occur-after-a-bout-of-maximal-intensity-contractions
#6
Gregory K Potts, Rachel M McNally, Rocky Blanco, Jae-Sung You, Alexander S Hebert, Michael S Westphall, Joshua J Coon, Troy A Hornberger
The maintenance of skeletal muscle mass is essential for health and quality of life. It is well recognized that maximal-intensity contractions, such as those which occur during resistance exercise, promote an increase in muscle mass. Yet, the molecules that sense the mechanical information and convert it into the signalling events (e.g. phosphorylation) that drive the increase in muscle mass remain undefined. Here we describe a phosphoproteomics workflow to examine the effects of electrically evoked maximal-intensity contractions (MIC) on protein phosphorylation in mouse skeletal muscle...
May 25, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28515850/dissection-of-z-disc-myopalladin-gene-network-involved-in-the-development-of-restrictive-cardiomyopathy-using-system-genetics-approach
#7
Qingqing Gu, Uzmee Mendsaikhan, Zaza Khuchua, Byron C Jones, Lu Lu, Jeffrey A Towbin, Biao Xu, Enkhsaikhan Purevjav
AIM: To investigate the regulation of Myopalladin (Mypn) and identify its gene network involved in restrictive cardiomyopathy (RCM). METHODS: Gene expression values were measured in the heart of a large family of BXD recombinant inbred (RI) mice derived from C57BL/6J and DBA/2J. The proteomics data were collected from Mypn knock-in and knock-out mice. Expression quantitative trait locus (eQTL) mapping methods and gene enrichment analysis were used to identify Mypn regulation, gene pathway and co-expression networks...
April 26, 2017: World Journal of Cardiology
https://www.readbyqxmd.com/read/28513514/efficacy-of-polishing-kits-on-the-surface-roughness-and-color-stability-of-different-composite-resins
#8
H Kocaagaoglu, T Aslan, A Gürbulak, H Albayrak, Z Taşdemir, H Gumus
OBJECTIVE: Different polishing kits may have different effects on the composite resin surfaces. The aim of this study was to evaluate the surface roughness and color stability of four different composites which was applied different polishing technique. MATERIALS AND METHODS: Thirty specimens were made for each composite resin group (nanohybrid, GrandioSo-GS; nanohybrid, Clearfil Majesty Esthetic-CME; hybrid, Valux Plus-VP; micro-hybrid, Ruby Comp-RC; [15 mm in diameter and 2 mm height]), with the different monomer composition and particle size from a total of 120 specimens...
May 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/28510117/novex-3-the-tiny-titin-of-muscle
#9
REVIEW
Dalma Kellermayer, John E Smith, Henk Granzier
The giant multi-functional striated muscle protein titin is the third most abundant muscle protein after myosin and actin. Titin plays a pivotal role in myocardial passive stiffness, structural integrity and stress-initiated signaling pathways. The complete sequence of the human titin gene contains three isoform-specific mutually exclusive exons [termed novel exons (novex)] coding for the I-band sequence, named novex-1 (exon 45), novex-2 (exon 46) and novex-3 (exon 48). Transcripts containing either the novex-1 or novex-2 exons code for the novex-1 and novex-2 titin isoforms...
April 7, 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/28499013/gantry-angulation-effects-on-ct-dose-along-the-z-axis-direction-in-head-examinations
#10
Masoumeh Parsi, Mehdi Sohrabi, Fereidoun Mianji, Reza Paydar
The purpose of this study was to evaluate the effects of the gantry angulation on dose profiles along the z-axis, the CTDIW value and the CTDIW efficiency in the head examinations. A Monte Carlo simulation model of a GE LightSpeed 16-slice CT scanner was developed by a GATE toolkit. The CTDI100 in air at the isocenter and in a head dosimetry phantom were measured using a pencil ion chamber. Dose profiles were studied in the central and peripheral holes of the head phantom using thermoluminescence LiF disc dosemeters for gantry angulations of 0°, 10° and 20°...
May 12, 2017: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/28495754/phd3-is-a-transcriptional-coactivator-of-hif-1%C3%AE-in-nucleus-pulposus-cells-independent-of-the-pkm2-jmjd5-axis
#11
Zachary R Schoepflin, Elizabeth S Silagi, Irving M Shapiro, Makarand V Risbud
The role of prolyl hydroxylase (PHD)-3 as a hypoxia inducible factor (HIF)-1α cofactor is controversial and remains unknown in skeletal tissues. We investigated whether PHD3 controls HIF-1 transcriptional activity in nucleus pulposus (NP) cells through the pyruvate kinase muscle (PKM)-2-Jumonji domain--containing protein (JMJD5) axis. PHD3(-/-) mice (12.5 mo old) showed increased incidence of intervertebral disc degeneration with a concomitant decrease in expression of the HIF-1α targets VEGF-A, glucose transporter-1, and lactate dehydrogenase A...
May 11, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28482435/-expression-of-nf-%C3%AE%C2%BAb-in-a-degenerative-human-intervertebral-disc-model
#12
X F Wang, A P Zhang, Z Y Sun, C Liu, L H Kuang, J W Tian
Objective: To investigate the changes in the expression of NF-κB signaling pathway in human degenerative intervertebral discs. Methods: From October 2014 to March 2016, 55 nucleus pulposus of surgical patients with degenerative human intervertebral disc were collected for study in Department of Orthopedic Surgey, Hospital of Zaozhuang Mining Corporation, and Department of Orthopedics, Shanghai General Hospital Affiliated Shanghai Jiaotong University, School of Medicine.The collected nucleus pulposus tissues were divided into two groups: experimental group(30) and control group(25)...
May 9, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28455287/desmin-loss-and-mitochondrial-damage-precede-left-ventricular-systolic-failure-in-volume-overload-heart-failure
#13
Jason Guichard, Michael Paul Rogowski, Giulio Agnetti, Lianwu Fu, Pamela Powell, Chih-Chang Wei, James F Collawn, Louis J Dell'Italia
BACKGROUND: Heart failure due to chronic volume overload (VO) in the rat and human is characterized by disorganization of cardiomyocyte desmin/mitochondrial network. Here we test the hypothesis that desmin breakdown is an early and continuous process throughout VO. METHODS: Male Sprague-Dawley rats had aortocaval fistula (ACF) or sham surgery for 24 hours, 4 weeks or 12 weeks (wk). Desmin/mitochondrial ultrastructure was examined by transmission electron microscopy (TEM) and immunohistochemistry (IHC)...
April 28, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28427137/-bibliometric-analysis-of-scientific-articles-on-epidemiological-study-of-burns-in-china
#14
W F Cheng, Z A Shen, D X Zhao, D W Li, Y R Shang
Objective: To analyze the current status of epidemiological study of burns in China, and to explore the related strategies. Methods: Retrospective or cross-sectional scientific articles in Chinese or English on epidemiological study of burns in China published from January 2005 to December 2015 were systemically retrieved from 4 databases. The databases include PubMed, Embase, China Biology Medicine disc, and Chinese Journals Full-text Database. From the results retrieved, data with regard to publication year, journal distribution, number of institutions participated in the study, affiliation of the first author and its location, and admission time span and age of patients in all the scientific articles were collected...
April 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28420175/a-new-input-device-for-spastics-based-on-strain-gauge
#15
Niels Buchhold, Christian Baumgartner
This article presents a new sensor for use by people with spastic disorders and similar conditions and enables them to steer and control medical devices such as electric powered wheelchairs. As spastic patients often suffer from cramping of their extremities, which can then no longer be controlled, using a standard joystick while operating a powered wheelchair can lead to dangerous situations. To prevent this, we designed a sensor based on strain gauges, which is shaped like a flat disc that can be operated using any body part...
April 17, 2017: Sensors
https://www.readbyqxmd.com/read/28416834/-efficacy-of-transforaminal-endoscopic-nerve-root-decompression-in-the-treatment-of-degenerative-lumbar-spinal-stenosis
#16
Z R Yu, C D Li, S N Zhu, H L Sun, Y Zhao, L T Qi
OBJECTIVE: To evaluate the feasibility of transforaminal endoscopic nerve root decompression for degenerative lumbar spinal stenosis (DLSS). METHODS: From July 2011 to April 2016, 96 cases of single segment DLSS were involved. All the patients had unilateral lower extremity neurological symptoms, signs, neurogenic intermittent claudication of less than 500 m. Imaging examinations (CT or MRI) or diagnostic nerve root block confirmed single segment degeneration. The mean age was (71...
April 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28411110/impaired-wnt-signaling-and-the-spine-heterozygous-wnt1-mutation-causes-severe-age-related-spinal-pathology
#17
Riikka E Mäkitie, Tuukka Niinimäki, Miika T Nieminen, Camilla Schalin-Jäntti, Jaakko Niinimäki, Outi Mäkitie
BACKGROUND: WNT signaling plays a major role in bone and cartilage metabolism. Impaired WNT/β-catenin signaling leads to early-onset osteoporosis, but specific features in bone and other tissues remain inadequately characterized. We have identified two large Finnish families with early-onset osteoporosis due to a heterozygous WNT1 mutation c.652T>G, p.C218G. This study evaluated the impact of impaired WNT/β-catenin signaling on spinal structures. METHODS: Altogether 18 WNT1 mutation-positive (age range 11-76years, median 49years) and 14 mutation-negative subjects (10-77years, median 43years) underwent magnetic resonance imaging (MRI) of the spine...
April 11, 2017: Bone
https://www.readbyqxmd.com/read/28380669/antibacterial-and-bioactive-coatings-on-titanium-implant-surfaces
#18
Anupama Kulkarni Aranya, Smruti Pushalkar, Minglei Zhao, Racquel Z LeGeros, Yu Zhang, Deepak Saxena
Various surface modifications have been tried for enhancing osseointegration of the dental implants like mechanical and/or chemical treatments and deposition of calcium phosphate coatings. The objective of this research was to develop calcium-phosphate based thin coatings with antibacterial and bioactive properties for potential application in dental implants. Titanium (Ti) discs were immersed in different calcifying solutions: CaP (positive control), F-CaP, Zn-CaP and FZn-CaP and incubated for 24 h. Negative control was uncoated Ti discs...
April 5, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28378073/kinematics-of-cervical-segments-c5-c6-in-axial-rotation-before-and-after-total-disc-arthroplasty
#19
Martin Michael Wachowski, Jan Weiland, Markus Wagner, Riccardo Gezzi, Dietmar Kubein-Meesenburg, Hans Nägerl
PURPOSE: The kinematical properties of C5/C6 segments in axial rotation are evaluated before and after total disc arthroplasty (TDA) with PRESTIGE(®)-and BRYAN(®) Cervical Disc (Medtronic) under flexion/extension as parameters and compared with those of C3/C4. METHODS: Eight human segments were stimulated by triangularly varying, axially directed torque (T z(t)) under compressing static axial preloads. Using a 6D-measuring device with high resolution, the response of segmental motion was characterized by the instantaneous helical axis (IHA)...
April 4, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28355743/-topping-off-surgery-versus-double-segment-fusion-for-treatment-of-lumbar-degenerative-disease-with-mid-long-term-follow-up
#20
X L Chen, Y Hai, L Guan, Y Z Liu, J C Yang, Q J Su, N Kang, X L Meng, L Yang, Y Wang
Objective: To compare the mid-long term clinical effect of Topping-off surgery and lumbar fusion surgery for two-segmental lumbar degenerative disease. Methods: From March 2009 to March 2012, one hundred and twenty-six consecutive patients (Topping-off surgery and two-segment PLIF surgery) were studied in Orthopedics Department, Beijing Chao-Yang Hospital, Capital Medical University.The VAS and ODI were used to assess clinical symptoms.All patients underwent flexion/extension radiographs examinations before surgery, 1, 2 years and last follow-up postoperatively...
March 21, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
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