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Franco H Falcone, Daniel Wan, Nafal Barwary, Ronit Sagi-Eisenberg
Since their establishment in 1981, RBL-2H3 cells have been widely used as a mast cell (MC) model. Their ability to be easily grown in culture in large amounts, their responsiveness to FcεRI-mediated triggers and the fact that they can be genetically manipulated, have provided advantages over primary MCs, in particular for molecular studies relying on genetic screening. Furthermore, the ability to generate clones that stably express proteins of interest, for example, a human receptor, have marked the RBL cells as an attractive MC model for drug screening...
March 2018: Immunological Reviews
Liangtao Lv, Lin Hong, Zhenxing Li, Ishfaq Ahmed, Guanzhi Chen
BACKGROUND: Food processing effects could modify the protein functional properties. However, protein was oxidized inevitably by lipid peroxidation during food processing. Acrolein, a primary by-product of lipid peroxidation, could modify the structural and functional properties of protein. The aim of the research is to analyze the effect of acrolein on allergenicity of TM, a major allergen in shrimp. RESULTS: The overall allergenic effects of acrolein treated TM were evaluated using female BALB/c mice and a mediator-releasing RBL-2H3 cell line...
February 10, 2018: Journal of the Science of Food and Agriculture
Jeong-Min Lim, Bina Lee, Ju-Hee Min, Eun-Young Kim, Jae-Hyun Kim, SooYeon Hong, Jwa-Jin Kim, Youngjoo Sohn, Hyuk-Sang Jung
Peiminine (PMN) is the main component derived from Fritillaria ussuriensis and is used in traditional medicine in East Asia. The aim of this study was to evaluate the effects of PMN on atopic dermatitis (AD) induced by a dinitrochlorobenzene (DNCB) in Balb/c mice. Inflammatory cytokine expression of PMN was investigated in vitro. Eosinophil infiltration and the thickness of DNCB-induced AD mouse skin were measured. The levels of IgE, IL-4, IL-6, IL-13, and TNF-α in the serum were measured by ELISA. The effects of PMN on the transcription level of MAPK and nuclear factor (NF)-κB were evaluated in mouse skin...
February 1, 2018: International Immunopharmacology
Shengli Han, Yanni Lv, Liyun Kong, Yueming Sun, Jia Fu, Lingjun Li, Langchong He
Traditional Chinese medicine (TCM) has been used for prevention and treatment of various diseases for many decades. TCM injection is a new dosage form, with incidence of anaphylactoid reactions increasing every year. In this study, the rat basophilic leukemia 2H3 (RBL-2H3) and laboratory of allergic disease 2 (LAD2) dual-mixed/CMC was established and was coupled with an HPLC-ESI-IT-TOF-MS system to identify the potential allergenic components in Haqing injection. Cinobufagin, piperine, osthole, praeruptorin A, and schizandrin A were screened from Haqing injection via this coupled system...
January 22, 2018: Electrophoresis
Siwen Niu, Qingmei Liu, Jin-Mei Xia, Chun-Lan Xie, Zhu-Hua Luo, Zongze Shao, Guang-Ming Liu, Xian-Wen Yang
To discover anti-food allergic components from deep-sea-derived microorganisms. A systematic chemical investigation was conducted on the Atlantic hydrothermal fungus Graphostroma sp. MCCC 3A00421. Consequently, nine new (1-9) and 19 known (10‒28) polyketides were isolated. The planar structures of the new compounds were elucidated mainly by detailed analysis of their NMR and HRESIMS data, while the absolute configurations were established using the modified Mosher's method in association with ECD spectra...
January 22, 2018: Journal of Agricultural and Food Chemistry
Toshiro Noshita, Kaori Miura, Kaoru Ikeda, Hidekazu Ouchi, Takuya Matsumoto, Akihiro Tai
The incidence of type I allergies, which are associated with mast cell degranulation and local inflammation, is increasing, and new treatments are needed. To date, structure-activity relationships of flavonoids in their degranulation-inhibiting activity have not been systematically characterized. In the current study, the degranulation-inhibiting activity of a series of flavonoids was evaluated. The following three observations were made: (1) the activity disappears when a sugar moiety is introduced into the A ring of the flavanone; (2) the activity depends on the number of hydroxyl groups on the B ring; (3) the activity is markedly enhanced when a double bond is introduced into the C ring...
January 18, 2018: Journal of Natural Medicines
Toshio Morikawa, Ikuko Hachiman, Kiyofumi Ninomiya, Hiroki Hata, Kaoru Sugawara, Osamu Muraoka, Hisashi Matsuda
A methanol extract of mace, the aril of Myristica fragrans (Myristicaceae), was found to inhibit the release of β-hexosaminidase, a marker of antigen-IgE-stimulated degranulation in rat basophilic leukemia cells (RBL-2H3, IC50 = 45.7 μg/ml). From the extract, three new 8-O-4' type neolignans, maceneolignans I-K (1-3), were isolated, and the stereostructures of 1-3 were elucidated based on spectroscopic and chemical evidence. Among the isolates, maceneolignans A (5), D (6), and H (8), (-)-(8R)-∆8'-4-hydroxy-3,3',5'-trimethoxy-8-O-4'-neolignan (13), (-)-(8R)-∆8'-3,4,5,3',5'-pentamethoxy-8-O-4'-neolignan (14), (-)-erythro-(7R,8S)-∆8'-7-acetoxy-3,4-methylenedioxy-3',5'-dimethoxy-8-O-4'-neolignan (17), (+)-licarin A (20), nectandrin B (24), verrucosin (25), and malabaricone C (29) were investigated as possible degranulation inhibitors (IC50 = 20...
January 15, 2018: Journal of Natural Medicines
Thanh Sang Vo, Se-Kwon Kim, BoMi Ryu, Dai Hung Ngo, Na-Young Yoon, Long Giang Bach, Nguyen Thi Nhat Hang, Dai Nghiep Ngo
UV light, especially UVB, is known as a trigger of allergic reaction, leading to mast cell degranulation and histamine release. In this study, phlorotannin Fucofuroeckol-A (F-A) derived from brown algal Ecklonia stolonifera Okamura was evaluated for its protective capability against UVB-induced allergic reaction in RBL-2H3 mast cells. It was revealed that F-A significantly suppress mast cell degranulation via decreasing histamine release as well as intracellular Ca2+ elevation at the concentration of 50 μM...
January 4, 2018: Marine Drugs
Qiaoling Fei, Yixin Han, Ruijuan Qi, Yuan Gao, Lei Fang, Rui Hou, Runlan Cai, Yun Qi
BACKGROUND: Basophilic granulocytes (BGs) not only initiate the induction of Th2 cell differentiation, but also amplify the ongoing Th2 response. Shuang-Huang-Lian (SHL) is clinically used for relieving type I hypersensitivity by continuous treatment for several weeks. METHODS: ELISA, flow cytometry, magnetic activated cell sorting, isoelectric precipitation, hybridoma technique, transfection and luciferase reporter assay were used in this study. The statistical analysis was performed using a one-way ANOVA...
January 3, 2018: BMC Complementary and Alternative Medicine
Fangchao Cui, Pei Zhu, Jian Ji, Ivana Blaženović, Morteza Gholami, Yinzhi Zhang, Xiulan Sun
Lipopolysaccharide (LPS) can lead to uncontrollable cytokine production, fatal sepsis syndrome and depression/multiple organ failure, as pathophysiologic demonstration. Various toxic effects of LPS have been extensively reported, mainly on the toxicity of LPS in cellular level, macrophages or tumor cells, etc. This work aimed on the impact of LPS on mast cell metabolism, which focused on LPS-induced cellular metabolic profiles. Gas chromatography-mass spectrometry (GC-MS) based metabolomics strategy was implemented for the endo-metabolites detection in rat basophilic leukemia (RBL-2H3) cells, treated with 10 μg/mL LPS for 24 h, along with multiple time-dose tests of cells viability/apoptosis...
December 21, 2017: Chemico-biological Interactions
Sullim Lee, No-June Park, Sim-Kyu Bong, Jonghwan Jegal, Sang-A Park, Su-Nam Kim, Min-Hye Yang
ETHNOPHARMACOLOGICAL RELEVANCE: The fruits of Juniperus rigida have been used in Korean traditional medicine for the treatment of inflammatory diseases in humans such as rheumatoid arthritis. AIM OF THE STUDY: This study aimed to investigate the anti-atopic properties of J. rigida fruit in in vivo murine atopic dermatitis (AD) models. METHODS AND RESULTS: BALB/c mouse ears ad SKH-1 hairless mice stimulated with oxazolone (4 weeks) and DNCB (3 weeks), respectively, were treated with the 1% Juniperus rigida fruit EtOH extract (JFE)...
December 16, 2017: Journal of Ethnopharmacology
Hironori Higashio, Yoh-Ichi Satoh, Tomoyuki Saino
Secretory granules (SGs) of mast cells are lysosome-related organelles that contain various inflammatory molecules such as histamine, which are stored in the cytoplasm. Mast cell degranulation is the regulated exocytosis of SGs in response to external stimuli, such as the antigen-mediated cross-linking of the high-affinity IgE receptor, FcεRI. Upon stimulation, SGs undergo priming to become fusion-competent prior to fusing with the plasma membrane, which is mediated by Munc13-4, one of the five members of the vesicle-priming Munc13 protein family...
2017: Biomedical Research
Hyun Ju Do, Tae Woo Oh, Ju Hye Yang, Kwang Il Park, Jin Yeul Ma
Davallia mariesii Moore (Drynaria rhizome extract (DRE)) is widely known for its efficacy in treating inflammation, arteriosclerosis, and bone injuries. This study evaluated whether treatment with DRE inhibited FcɛRI-mediated allergic responses in the RBL-2H3 mast cells and investigated the early- and late-phase mechanisms by which DRE exerts its antiallergic effects. IgE anti-DNP/DNP-HSA-sensitized RBL-2H3 mast cells were tested for cytotoxicity to DRE, followed by the assessment of β-hexosaminidase release...
2017: Mediators of Inflammation
Songsong Jiang, Yifan Da, Shiwen Han, Yahong He, Huilian Che
Food allergy includes sensitization phase and effect phase, and effect cells degranulate and secrete cytokines in the effect phase, causing allergic clinical symptoms. We have demonstrated that Notch signaling plays an important role in the sensitization phase, but its role in effect phases still remains unclear. In this study, we investigated the role of Notch signaling in degranulation and cytokine production of the effect phase response. A RBL-2H3 cell model was used and Notch signaling was induced by priming with Notch ligands...
November 28, 2017: Immunologic Research
Donglei Jiang, Yan Liu, Hui Jiang, Shengqi Rao, Wu Fang, Mangang Wu, Limin Yuan, Weiming Fang
A novel screen-printed cell-based electrochemical sensor was developed to assess bacterial quorum signaling molecules, N-acylhomoserine lactones (AHLs). Screen-printed carbon electrode (SPCE), which possesses excellent properties such as low-cost, disposable and energy-efficient, was modified with multi-walled carbon nanotubes (MWNTs) to improve electrochemical signals and enhance the sensitivity. Rat basophilic leukemia (RBL-2H3) mast cells encapsulated in alginate/graphene oxide (NaAgl/GO) hydrogel were immobilized on the MWNTs/SPCE to serve as recognition element...
November 11, 2017: Biosensors & Bioelectronics
Xiaoqian Yu, Xiaotong Sun, Meng Zhao, Yonghao Hou, Jingxin Li, Jingui Yu, Yuedong Hou
Cardiac mast cell activation is involved in the process of myocardial ischemia reperfusion (I/R) injury and exacerbates myocardial infarction. Propofol, an anesthetic with antioxidant property, can reduce myocardial infarct size in I/R injury. The present study was designed to investigate whether propofol can attenuate myocardial I/R injury by inhibiting resident cardiac mast cell activation by a Langendorff model. Thirty rats were randomly assigned to 5 groups (n=6 per group): control group and four test groups (I/R, I/R+compound 48/80, I/R+propofol, I/R+compound 48/80+propofol)...
November 21, 2017: International Immunopharmacology
Bo-Kyung Lee, Soo-Jin Park, So-Yeon Nam, Saeromi Kang, Jin Hwang, Seung-Jin Lee, Dong-Soon Im
ETHNOPHARMACOLOGICAL RELEVANCE: Saussurea costus (Falc.) Lipsch. root has been used in Asian traditional medicine for the treatment of asthma, rheumatism, and other conditions. S. costus extracts were shown to alleviate house dust mite-induced atopic-like dermatitis in Nc/Nga mice; besides, sesquiterpene lactones were isolated from S. costus extracts. AIMS OF THE STUDY: We aimed to investigate the effects of sesquiterpene lactones (alantolactone, costunolide, and dehydrocostuslactone) in allergic asthma using female Balb/c mice and rat RBL-2H3 mast cells...
November 17, 2017: Journal of Ethnopharmacology
Yuanyuan Gao, Qingmei Liu, Bo Liu, Chun-Lan Xie, Min-Jie Cao, Xian-Wen Yang, Guang-Ming Liu
Compounds of the deep-sea-derived marine Williamsia sp. MCCC 1A11233 (CDMW), which are secondary metabolites of the actinomycetes, were isolated. In this study, seven kinds of CDMW were found to decrease degranulation and histamine release in immunoglobulin E (IgE)-mediated rat basophilic leukemia (RBL)-2H3 cells. The production of cytokines (tumor necrosis factor-α and interleukin-4) was inhibited by these CDMW in RBL-2H3 cells, and their chemical structures were established mainly based on detailed analysis of their NMR spectra...
November 17, 2017: Journal of Agricultural and Food Chemistry
Jung Ae Kang, Ha-Yeon Song, Eui-Hong Byun, Nam-Geun Ahn, Hye-Min Kim, You Ree Nam, Gyeong Hee Lee, Beom-Su Jang, Dae Seong Choi, Dong-Eun Lee, Eui-Baek Byun
Gamma irradiation is able to affect various structural modification and an increase of the biological properties of biomaterials. This study was conducted to investigate the anti-allergenic effect of γ-irradiated black ginseng extract (BGE) using in vitro and in vivo experiments. IgEantigen complex-induced degranulation was measured in RBL-2H3 mast cells. In addition, an anti-atopic dermatitis (AD) test was carried out by spreading γ-irradiated BGE on the dorsal skin of 2,4-dinitrochlorobenzene (DNCB)-induced BALB/c mice...
November 7, 2017: Food and Chemical Toxicology
Haoyue Yang, Masatoshi Honda, Akiko Saito, Taira Kajisa, Yuhki Yanase, Toshiya Sakata
In this study, we report the label-free and reliable detection of allergic response using a cell-coupled gate field-effect transistor (cell-based FET). Rat basophilic leukemia (RBL-2H3) cells were cultured as a signal transduction interface to induce allergic reaction on the gate oxide surface of the FET, because IgE antibodies, which bind to Fcε receptors at the RBL-2H3 cell membrane, are specifically cross-linked by allergens, resulting in the allergic response of RBL-2H3 cells. In fact, the surface potential at the FET gate decreased owing to secretions such as histamine from the IgE-bound RBL-2H3 cells, which reacted with the allergen...
November 15, 2017: Analytical Chemistry
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