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Mariana Barbosa, Graciliana Lopes, Patrícia Valentão, Federico Ferreres, Ángel Gil-Izquierdo, David M Pereira, Paula B Andrade
The anti-allergenicity of phlorotannin-targeted extracts from four edible seaweed species of Fucus genus was evaluated herein for the first time. Extracts were able to act upon cellular events triggered by immunological reaction (IgE/antigen), and on cellular events downstream the Ca2+ influx caused by a chemical stimulus (calcium ionophore A23187), preventing degranulation of RBL-2H3 cells. Furthermore, a dose-dependent behaviour towards allergy-related enzymatic systems was observed for all the phlorotannin extracts...
November 1, 2018: Food Chemistry
Yi-Hsuan Lin, Hsing-Yu Chen, Jung-Chun Chiu, Kun-Jei Chen, Hung-Yao Ho, Sien-Hung Yang
Schizonepeta tenuifolia (ST) Briq. is a traditional herbal medicine commonly used to treat allergic skin diseases, where the inflammation process is closely related to symptom severity. This study aimed to explore the immunomodulatory effect of ST by using immunoglobulin E- (IgE-) stimulated RBL-2H3 cell cultures, a common cell line for studying mast cell degranulation and inflammatory cytokine release in vitro . After stimulating the RBL-2H3 cells with IgE, ST at concentrations of 10, 50, or 100  μ g/mL was added to the cell cultures...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Zhenjiang Xia, Lixin Wei, Yuzhi Du, Lulu Wang, Jichun Han, Qiangqiang He, Haijuan Chen, Xiaoyan Zou, Huali Wu, Jing Shang
Zuotai is a drug containing mercury considered to be the king of Tibetan medicine. The biosafety of Zuotai led people's attention and so far little is known about the toxicity of Zuotai to mast cells. RBL-2H3 cells which used as an alternative model of mast cells were treated with Zuotai, β-HgS and positive drug Compound 48/80 respectively. MTT assay was used to determine the toxicity of drugs to RBL-2H3 cells. The degranulation of RBL-2H3 cells was studied from β-hexosaminidase, histamine, IL-4 and TNF-α...
May 31, 2018: Chemical & Pharmaceutical Bulletin
Tian-Yu Yu, Zhi-Gang He, Mu-Qing Yang, Jian Song, Cheng Ma, Sun-Qiang Ma, Jun-Lan Feng, Bin Liu, Xiao-Dong Wang, Ji-Yu Li, Zhu-Bo Wei
Mast cells (MCs) are tissue-based stationary effector cells that form the immune system's first-line defense against various challenges. They are developed from the bone marrow-derived progenitors to complete their differentiation and maturation in the tissues where they eventually establish residence. MCs have been implicated in many diseases, such as allergy, parasitic infection, and neoplastic disorders. Immortalized MC lines, such as RBL-2H3, HMC-1, and LAD-2, are useful for investigating the biological functions of MC only to some extents due to the restriction of degranulation evaluation, in vivo injection and other factors...
May 26, 2018: Experimental Cell Research
Rishipal R Bansode, Nathalie J Plundrich, Priscilla D Randolph, Mary Ann Lila, Leonard L Williams
This study investigates the anti-allergic properties of peanut skin polyphenols (PSP)-enriched peanut (PN) protein aggregates. PSP was blended with PN flour at concentrations of 5, 10, 15, 30, and 40% (w/w). Rat basophil leukemia cells (RBL-2H3) were sensitized with either anti-DNP-IgE or PN-allergic plasma followed by co-exposure to unmodified PN flour (control) or PSP-PN protein aggregates and Ca2+ ionophore, ionomycin. Immunoblotting and staining were performed to measure the IgE binding capacity of PSP-PN aggregates...
October 15, 2018: Food Chemistry
Young Hwan Park, Do Kyun Kim, Hyun Woo Kim, Hyuk Soon Kim, Dajeong Lee, Min Bum Lee, Keun Young Min, Jimo Koo, Su Jeong Kim, Changhee Kang, Young Mi Kim, Hyung Sik Kim, Wahn Soo Choi
Mast cells are critical effector cells in IgE-mediated allergic responses. The aim of this study was to investigate the anti-allergic effects of 3-[(aminocarbonyl)amino]-5-(3-fluorophenyl)-N-(3S)-3-piperidinyl-2-thiophenecarboxamide (AZD7762) in vitro and in vivo. AZD7762 inhibited the antigen-stimulated degranulation from RBL-2H3 (IC50 , ∼ 27.9 nM) and BMMCs (IC50 , ∼ 99.3 nM) in a dose-dependent manner. AZD7762 also inhibited the production of TNF-α and IL-4. As the mechanism of its action, AZD7762 inhibited the activation of Syk and its downstream signaling proteins, such as Linker of activated T cells (LAT), phospholipase (PL) Cγ1, Akt, and mitogen-activated protein (MAP) kinases (Erk1 / 2, p38, and JNK) in mast cells...
May 16, 2018: Biochemical Pharmacology
Xiang Dong Su, Rui Hong Guo, Hong Xu Li, Jin Yeul Ma, Young Ran Kim, Young Ho Kim, Seo Young Yang
Sanguisorba officinalis L. was well known as a traditional herbal medicine to treat inflammation and allergic skin diseases. The aim of this research was to indentify compounds with anti-allergic inflammatory property. Twenty-five compounds (1-25) were isolated from S. officinalis including two new compounds (1 and 8), and their chemical structures were identified by NMR and ESIMS analysis. Consequently, the anti-allergic inflammatory activities of these isolates were investigated by inhibiting β-hexosaminidase and IL-4 production in PMA/A23187-stimulated RBL-2H3 cells...
April 12, 2018: Bioorganic & Medicinal Chemistry Letters
Hiroaki Hayashi, Ryusuke Kaneko, Shunsuke Demizu, Daichi Akasaka, Manabu Tayama, Takafumi Harada, Hiroki Irie, Yoshio Ogino, Naoko Fujino, Eiji Sasaki
Spleen tyrosine kinase (Syk) is involved in regulation of B-cell receptor (BCR) and Fc receptor (FcR) downstream signal pathways. Syk plays an essential role in production of inflammatory mediators and differentiation in various immune cells and is therefore an attractive target for treating inflammatory conditions, such as autoimmune and allergic diseases. We identified TAS05567 as a highly selective Syk inhibitor and evaluated its therapeutic potential in animal models. In vitro biochemical assays were performed with available kinase assay panels...
May 4, 2018: Journal of Pharmacology and Experimental Therapeutics
Jin Huang, Mingzhi Su, Bo-Kyung Lee, Mee-Jeong Kim, Jee H Jung, Dong-Soon Im
4-Hydroxy-2-(4-hydroxyphenethyl)isoindoline-1,3-dione (PD1) is a synthetic phthalimide derivative of a marine compound. PD1 has peroxisome proliferator-activated receptor (PPAR) γ agonistic and anti-inflammatory effects. This study aimed to investigate the effect of PD1 on allergic asthma using rat basophilic leukemia (RBL)-2H3 mast cells and an ovalbumin (OVA)-induced asthma mouse model. In vitro , PD1 suppressed β-hexosaminidase activity in RBL-2H3 cells. In the OVA-induced allergic asthma mouse model, increased inflammatory cells and elevated Th2 and Th1 cytokine levels were observed in bronchoalveolar lavage fluid (BALF) and lung tissue...
April 18, 2018: Biomolecules & Therapeutics
Ju-Hye Yang, Hyun Ju Do, Esther Lee, Nam-Hui Yim, Won-Kyung Cho, Kwang-Il Park, Jin Yeul Ma
ETHNOPHARMACOLOGICAL RELEVANCE: Jageum-Jung (JGJ) is an oriental herbal formula comprising five herbs (Melaphis chinensis Bell, Cremastra variabilis Nakai, Knoxia valerianoides Thorel, Euphorbia lathyris L., and Moschus moschiferus L.). It has been used for detoxification and treating cancer and inflammatory diseases in China, Japan, and Korea. However, the mechanism of action of JGJ on keratinocyte inflammatory response is poorly understood. AIM OF THE STUDY: In the present study, we investigated the anti-inflammatory mechanism of JGJ and studied the effects of JGJ on atopic dermatitis-like skin lesions in mice...
July 15, 2018: Journal of Ethnopharmacology
Beom-Geun Jo, No-June Park, Jonghwan Jegal, Sangho Choi, Sang Woo Lee, Hang Jin, Su-Nam Kim, Min Hye Yang
Stellera chamaejasme L. (family Thymelaeaceae), also known as 'Langdu', has been traditionally used to treat of skin-related diseases, such as, psoriasis and skin ulcers. The aim of this study was to identify the biologically active component of S. chamaejasme and evaluate its preventive effects on IL-4 and mast cell degranulation in RBL-2H3 cells and on the development of atopic dermatitis (AD) in 2,4-dinitrochlorobenzene (DNCB)-treated SKH-1 hairless mice. A novel flavonoid, genkwanin 5-O-xylosyl(1 → 2)glucoside (named stechamone), and three known compounds (umbelliferone, luteolin, and luteolin-7-O-glucoside) were isolated from the aerial parts of S...
June 2018: International Immunopharmacology
Stephen Bruinsma, Declan J James, Melanie Quintana Serrano, Joseph Esquibel, Sang Su Woo, Elle Kielar-Grevstad, Ellen Crummy, Rehan Qurashi, Judy A Kowalchyk, Thomas F J Martin
Ca2+ -dependent secretory granule fusion with the plasma membrane is the final step for the exocytic release of inflammatory mediators, neuropeptides, and peptide hormones. Secretory cells use a similar protein machinery at late steps in the regulated secretory pathway, employing protein isoforms from the Rab, Sec1/Munc18, Munc13/CAPS, SNARE, and synaptotagmin protein families. However, no small-molecule inhibitors of secretory granule exocytosis that target these proteins are currently available but could have clinical utility...
May 25, 2018: Journal of Biological Chemistry
Hidetoshi Arima, Keiichi Motoyama, Taishi Higashi, Satoshi Fukushima, Hironobu Ihn
 Atopic dermatitis (AD) is a chronic skin disease characterized by inflammation, pruritus, and eczematous lesions. Recently, sacran, a new polysaccharide isolated from cyanobacterium Aphanothece sacrum (Sur.) OKADA discovered by Suringar in the 19th century, has received considerable attention as a novel biomaterial. Previously, it was reported that sacran exhibits potent anti-inflammatory effects for skin diseases produced by various stimulants with optimum effective concentrations at 0.01% (w/v) and 0.05% (w/v) in rats and mice...
2018: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
Pratikshya Adhikari, Hao Xu
Acknowledgements This work was supported by the University of Southern Mississippi Development Grant DE01475, the National Institute of Allergy and Infectious Diseases Grant 1R15AI133430-01, and by the Mississippi INBRE, which was funded by an Institutional Development Award (IDeA) from NIGMS under Grant no. P20GM103476.
June 2018: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
Wei-Lin Chen, Huan-Ling Chen, Gui-Wen Guo, Yu-Chun Huang, Cheng-Yu Chen, Yu Tsai, Keh-Feng Huang, Chao-Hsun Yang
Locust bean gum (LBG) galactomannan has been claimed to have applications in the biopharmaceutical field. However, the effects of LBG galactomannan on immunomodulatory aspects are not yet clear. The purpose of this study was to over-express thermostable β-d-mannanase from the thermophilic actinomycete Thermobifida fusca BCRC 19214 using a Pichia pastoris expression system. The maximum intracellular β-d-mannanase activity obtained from the cell-free extract was approximately 40.0U/mL after 72h of cultivating a P...
July 15, 2018: International Journal of Biological Macromolecules
Miku Maruyama, Miki Kobayashi, Takeshi Uchida, Eisaku Shimizu, Hironori Higashio, Misa Ohno, Shota Uesugi, Ken-Ichi Kimura
Amber is fossilized tree resin and several biologically active compounds were isolated from ambers using the growth-restoring activity of the mutant yeast [Saccharomyces cerevisiae (zds1∆ erg3∆ pdr1∆ pdr3∆)] involving Ca2+ -signal transduction. The aim of this study is to investigate the anti-allergic effect of both the methanol extract of Kuji amber (MEKA) and its main biologically active constituent, kujigamberol (15,20-dinor-5,7,9-labdatrien-18-ol) having activity against the mutant yeast. Both MEKA and kujigamberol inhibited the degranulation of RBL-2H3 cells by stimulation of thapsigargin (Tg) (IC50  = 15...
March 5, 2018: Fitoterapia
Seiji Awane, Kosuke Nishi, Momoko Ishida, Masanobu Nagano, Kazunori Hashiguchi, Akira Fujii, Takuya Sugahara
Japanese black vinegar (JBV) is a traditional vinegar manufactured with steamed unpolished rice. After screening, beneficial effects of JBV on IgE-mediated allergic responses were found. In this study, acetic acid-free JBV was used to evaluate its antiallergic effects. JBV suppressed degranulation of rat basophilic leukemia RBL-2H3 cells in a dose-dependent manner without cytotoxicity. The inhibitory effect of JBV on the degranulation seemed to be caused by the bioactive ingredients other than proteins, because the activity was not affected by heat treatment or protease digestion...
March 2, 2018: Cytotechnology
Kurt Appel, Eduardo Munoz, Carmen Navarrete, Cristina Cruz-Teno, Andreas Biller, Eva Thiemann
Immulina® , a high-molecular-weight polysaccharide extract from the cyanobacterium Arthrospira platensis ( Spirulina ) is a potent activator of innate immune cells. On the other hand, it is well documented that Spirulina exerts anti-inflammatory effects and showed promising effects with respect to the relief of allergic rhinitis symptoms. Taking into account these findings, we decided to elucidate whether Immulina® , and immunLoges® (a commercial available multicomponent nutraceutical with Immulina® as a main ingredient) beyond immune-enhancing effects, might also exert inhibitory effects in the induced allergic inflammatory response and on histamine release from RBL-2H3 mast cells...
February 26, 2018: Plants (Basel, Switzerland)
M A Chelombitko, O A Averina, T V Vasilyeva, O Yu Pletiushkina, E N Popova, A V Fedorov, B V Chernyak, V S Shishkina, O P Ilinskaya
The therapeutic effect of mitochondria-targeted antioxidant 10-(6´-plastoquinonyl)decyltriphenylphosphonium bromide (SkQ1) in experimental models of acute inflammation and wound repair has been shown earlier. It was suggested that the antiinflammatory activity of SkQ1 is related to its ability to suppress inflammatory activation of the vascular endothelium and neutrophil migration into tissues. Here, we demonstrated that SkQ1 inhibits activation of mast cells (MCs) followed by their degranulation and histamine release in vivo and in vitro...
December 2017: Biochemistry. Biokhimii︠a︡
Pieu Naskar, Nilofer Naqvi, Niti Puri
Mast cells (MCs) respond to allergen challenge by release of pre-stored inflammatory mediators from their secretory granules, on cross-linking of Fc(epsilon) receptor I (Fc(epsilon)RI) receptors. The target-SNARE (t-SNARE) SNAP-23 has been shown to play an important role in MC exocytosis and undergoes transient phosphorylation at Serine 95 (S95) and Serine 120 (S120), concomitant with mediator release. During current study we explored the importance of transient nature of phosphorylation at S120 in MC exocytosis...
March 2018: Journal of Biosciences
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