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https://www.readbyqxmd.com/read/28694291/differential-regulation-of-mas-related-g-protein-coupled-receptor-x2-mediated-mast-cell-degranulation-by-antimicrobial-host-defense-peptides-and-p-gingivalis-lps
#1
Kshitij Gupta, Chizobam Idahosa, Saptarshi Roy, Donguk Lee, Hariharan Subramanian, Anuradha Dhingra, Kathleen Boesze-Battaglia, Jonathan Korostoff, Hydar Ali
Porphyromonas gingivalis (P. gingivalis) is a keystone pathogen that contributes to periodontal pathogenesis by disrupting host-microbe homeostasis and promoting dysbiosis. Virulence of P. gingivalis likely reflects an alteration in the lipid A composition of its lipopolysaccharide (LPS) from the penta-acylated (PgLPS1690) to the tetra-acylated (PgLPS1435/1449) form. Mast cells play an important role in periodontitis but the mechanisms of their activation and regulation remain unknown. The expression of epithelium and neutrophil-derived host defense peptides (HDPs; LL-37, human β-defensin-3), which activate mast cells via Mas-related G protein coupled receptor X2 (MRGPRX2), is increased in periodontitis...
July 10, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28684600/frontline-science-corticotropin-releasing-factor-receptor-subtype-1-is-a-critical-modulator-of-mast-cell-degranulation-and-stress-induced-pathophysiology
#2
Saravanan Ayyadurai, Amelia J Gibson, Susan D'Costa, Elizabeth L Overman, Laura J Sommerville, Ashwini C Poopal, Emily Mackey, Yihang Li, Adam J Moeser
Life stress is a major risk factor in the onset and exacerbation of mast cell-associated diseases, including allergy/anaphylaxis, asthma, and irritable bowel syndrome. Although it is known that mast cells are highly activated upon stressful events, the mechanisms by which stress modulates mast cell function and disease pathophysiology remains poorly understood. Here, we investigated the role of corticotropin-releasing factor receptor subtype 1 (CRF1) in mast cell degranulation and associated disease pathophysiology...
July 6, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28659175/allergens-in-red-ginseng-extract-induce-the-release-of-mediators-associated-with-anaphylactoid-reactions
#3
Lu Wang, Yazheng Zhao, Ye Yang, Yuanjia Hu, Xiaohan Zou, Boyang Yu, Jin Qi
BACKGROUND: Anaphylactoid reactions induced by preparations containing red ginseng have been reported. The aim of this study is to evaluate the allergenicity and screen potential allergens in red ginseng extract thoroughly. METHODS: Red ginseng extract (RGE) and different fractions of RGE were prepared and evaluated by measuring the degranulation and viability of rat basophilic leukemia 2H3 (RBL-2H3) cells. Potential allergens were screened by RBL-2H3 cell extraction and allergenicity verified in RBL-2H3 cells, mouse peritoneal mast cells, Laboratory of Allergic Disease 2 (LAD2) human mast cells and mice, respectively...
June 28, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28623338/high-resolution-imaging-of-a-cell-attached-nanointerface-using-a-gold-nanoparticle-two-dimensional-sheet
#4
Shihomi Masuda, Yuhki Yanase, Eiji Usukura, Sou Ryuzaki, Pangpang Wang, Koichi Okamoto, Thasaneeya Kuboki, Satoru Kidoaki, Kaoru Tamada
This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28527346/inhibitory-effects-of-2-oxo-2h-chromen-4-yl-4-methylbenzenesulfonate-on-allergic-inflammatory-responses-in-rat-basophilic-leukemia-cells
#5
Gaeun Yoo, Kooyeon Lee, Deug-Chan Lee
Mast cells play crucial roles in the initiation of allergic inflammatory responses by releasing various mediators such as histamines, cytokines, and leukotrienes. In addition, signaling cascade pathways, such as the mitogen-activated protein kinase (MAPK) pathway, contribute to the regulation of mast cell degranulation. Accordingly, different research strategies have been pursued to develop anti-inflammatory and anti-allergic drugs by regulating these signaling pathways. The development of new drugs that inhibit mast cell degranulation may help in the treatment of allergies...
May 17, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28502305/-shikimic-acid-inhibits-the-degranulation-and-histamine-release-in-rbl-2h3-cells
#6
Xianyong Chen, Qianqian Zheng, Wei Liu, Lingling Yu, Jinling Wang, Shigang Li
Objective To study the effects of shikimic acid on the proliferation of rat RBL-2H3 cells and the degranulation of the cells induced by C48/80 and its mechanism. Methods MTT assay was performed to measure the proliferation of RBL-2H3 cells treated with 3, 10, 30 μg/mL shikimic acid. Toluidine blue staining was used to observe the degranulation of RBL-2H3 cells. The release of β-hexosaminidase from RBL-2H3 cells treated with 0, 12.5, 25, 50, 80, 100 μg/mL C48/80 was determined by substrate assay. ELISA was used to detect the histamine content in the supernatant of each treated group...
May 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28411215/the-effect-of-rho-drugs-on-mast-cell-activation-and-degranulation
#7
Avinash Sheshachalam, Alicia Baier, Gary Eitzen
Mast cells are tissue-resident immune cells that produce potent proinflammatory mediators, which are stored in cytoplasmic granules. Stimulation triggers degranulation, a process that mobilizes granules to dock and fuse to the plasma membrane, releasing mediators. Mast cell degranulation has an important role in immunity but can also intensify inflammation and contribute to allergic disorders. Hence, it is important to understand signaling pathways that regulate mast cell degranulation. Here, we examined the role of Rho proteins in regulating mast cell activation leading to degranulation...
April 14, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28393919/association-of-c-type-lectin-mincle-with-fc%C3%AE%C2%B5ri%C3%AE-%C3%AE-subunits-leads-to-functional-activation-of-rbl-2h3-cells-through-syk
#8
Chisato Honjoh, Kazuyasu Chihara, Hatsumi Yoshiki, Shota Yamauchi, Kenji Takeuchi, Yuji Kato, Yukio Hida, Tamotsu Ishizuka, Kiyonao Sada
Macrophage-inducible C-type lectin (Mincle) interacts with the γ-subunit of high-affinity IgE receptor (FcεRIγ) and activates Syk by recognizing its specific ligand, trehalose-6,6'-dimycolate, a glycolipid produced by Mycobacterium tuberculosis. It has been suggested that mast cells participate in the immune defense against pathogenic microbes including M. tuberculosis, although the functions are still uncertain. In this study, we examined the Mincle-mediated signaling pathway and cellular responses using RBL-2H3 cells...
April 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28367714/uncovering-the-molecular-mechanism-of-anti-allergic-activity-of-silkworm-pupa-grown-cordyceps-militaris-fruit-body
#9
Ting-Feng Wu, Yu-Yi Chan, Wan-Yin Shi, Meng-Ting Jhong
Cordyceps militaris has been widely used as an herbal drug and tonic food in East Asia and has also been recently studied in the West because of its various pharmacological activities such as antitumoral, anti-inflammatory and immunomodulatory effects. In this study, we examined the molecular mechanism underlying the anti-allergic activity of ethanol extract prepared from silkworm pupa-cultivated Cordyceps militaris fruit bodies in activated mast cells. Our results showed that ethanol extract treatment significantly inhibited the release of β-hexosaminidase (a degranulation marker) and mRNA levels of tumor necrosis factor-[Formula: see text] as well as interleukin-4 in RBL-2H3 cells...
April 2, 2017: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/28359154/eucheuma-cottonii-sulfated-oligosaccharides-decrease-food-allergic-responses-in-animal-models-by-up-regulating-regulatory-t-treg-cells
#10
Sha-Sha Xu, Qing-Mei Liu, An-Feng Xiao, Soheila J Maleki, Marcos Alcocer, Yuan-Yuan Gao, Min-Jie Cao, Guang-Ming Liu
In the present study, the anti-food allergy activity of Eucheuma cottonii sulfated oligosaccharide (ESO) was investigated. ESO was obtained by enzymatic degradation and purified by column chromatography. RBL-2H3 cells and BALB/c mouse model were used to test the anti-food allergy activity of ESO. The effects of ESO on the regulatory T (Treg) cells and bone marrow-derived mast cells (BMMCs) were investigated by flow cytometry. The results of in vivo assay showed that ESO decreased the levels of mast cell protease-1 and histamine and inhibited the levels of specific IgE by 77...
April 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28335419/anti-allergic-compounds-from-the-deep-sea-derived-actinomycete-nesterenkonia-flava-mccc-1k00610
#11
Chun-Lan Xie, Qingmei Liu, Jin-Mei Xia, Yuanyuan Gao, Quan Yang, Zong-Ze Shao, Guangming Liu, Xian-Wen Yang
A novel cyclic ether, nesterenkoniane (1), was isolated from the deep-sea-derived actinomycete Nesterenkonia flava MCCC 1K00610, together with 12 known compounds, including two macrolides (2, 3), two diketopiperazines (4, 5), two nucleosides (6, 7), two indoles (8, 9), three phenolics (10-12), and one butanol derivate (13). Their structures were established mainly on detailed analysis of the NMR and MS spectroscopic data. All 13 compounds were tested for anti-allergic activities using immunoglobulin E (IgE) mediated rat mast RBL-2H3 cell model...
March 14, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28327534/the-anti-allergic-rhinitis-effect-of-traditional-chinese-medicine-of-shenqi-by-regulating-mast-cell-degranulation-and-th1-th2-cytokine-balance
#12
Yang-Yang Shao, Yi-Ming Zhou, Min Hu, Jin-Ze Li, Cheng-Juan Chen, Yong-Jiang Wang, Xiao-Yun Shi, Wen-Jie Wang, Tian-Tai Zhang
Shenqi is a traditional Chinese polyherbal medicine has been widely used for the treatment of allergic rhinitis (AR). The aim of this study was to investigate the anti-allergic rhinitis activity of Shenqi and explore its underlying molecular mechanism. Ovalbumin (OVA)-induced allergic rhinitis rat model was used to evaluate the anti-allergic rhinitis effect of Shenqi. The effect of Shenqi on IgE-mediated degranulation was measured using rat basophilic leukemia (RBL-2H3) cells. Primary spleen lymphocytes were isolated to investigate the anti-allergic mechanism of Shenqi by detecting the expression of transcription factors via Western blot and the level of cytokines (IL-4 and IFN-γ) via ELISA...
March 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28327442/immune-cells-and-mediators-involved-in-the-inflammatory-responses-induced-by-a-p-i-metalloprotease-and-a-phospholipase-a2-from-bothrops-atrox-venom
#13
Danilo L Menaldo, Carolina P Bernardes, Karina F Zoccal, Anna L Jacob-Ferreira, Tássia R Costa, Maria P F M Del Lama, Rose M Z G Naal, Fabiani G Frantz, Lúcia H Faccioli, Suely V Sampaio
Bothrops envenomations can promote severe inflammatory responses by inducing edema, pain, leukocyte recruitment and release of chemical mediators by local cells. In the present study, two toxins from Bothrops atrox venom (the P-I metalloprotease Batroxase and the acidic phospholipase A2 BatroxPLA2) were evaluated in relation to their inflammatory effects induced in vivo and in vitro, mainly focusing on the participation of different immune cells and inflammatory mediators. Both toxins mainly promoted acute inflammatory responses with significant recruitment of neutrophils in the early hours (1-4h) after administration into the peritoneal cavity of C57BL/6 mice, and increased infiltration of mononuclear cells especially after 24h...
March 16, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28315853/effects-of-extracts-and-compounds-from-tricholoma-populinum-lange-on-degranulation-and-il-2-il-8-secretion-of-immune-cells
#14
Simon Merdivan, Kristina Jenett-Siems, Karsten Siems, Timo H J Niedermeyer, Nadin Schultze, Carmen Steinborn, Carsten Gründemann, Ulrike Lindequist
Tricholoma populinum Lange is an edible basidiomycete from the family Tricholomataceae. Extracts, fractions, and different metabolites isolated from the fruiting bodies of this mushroom were tested for degranulation-inhibiting activities on RBL-2H3 cells (rat basophils). Dichloromethane extracts decreased degranulation significantly, as did a fraction after column chromatography. In addition, the extract decreased the IL-2 release from Jurkat T cells and the release of IL-8 from HMC-1 human mast cells. The results show the significant effects of extracts of T...
July 14, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28294257/protein-bound-vaccinium-fruit-polyphenols-decrease-ige-binding-to-peanut-allergens-and-rbl-2h3-mast-cell-degranulation-in-vitro
#15
Nathalie J Plundrich, Rishipal R Bansode, E Allen Foegeding, Leonard L Williams, Mary Ann Lila
Peanut allergy is a worldwide health concern. In this study, the natural binding properties of plant-derived polyphenols to proteins was leveraged to produce stable protein-polyphenol complexes comprised of peanut proteins and cranberry (Vaccinium macrocarpon Ait.) or lowbush blueberry (Vaccinium angustifolium Ait.) pomace polyphenols. Protein-bound and free polyphenols were characterized and quantified by multistep extraction of polyphenols from protein-polyphenol complexes. Immunoblotting was performed with peanut-allergic plasma to determine peanut protein-specific IgE binding to unmodified peanut protein, or to peanut protein-polyphenol complexes...
April 19, 2017: Food & Function
https://www.readbyqxmd.com/read/28292010/the-anti-allergic-and-anti-inflammatory-effects-of-benjakul-extract-a-thai-traditional-medicine-its-constituent-plants-and-its-some-pure-constituents-using-in-vitro-experiments
#16
Sunita Makchuchit, Ruchilak Rattarom, Arunporn Itharat
Benjakul (BJK), a Thai traditional medicine preparation, has long been used for balanced health, controlled abnormal of element in the body, carminative, and relief of flatulence. It is composed of five plants: Piper interruptum Opiz., Piper longum L., Piper sarmentosum Roxb., Plumbago indica L., and Zingiber officinale Roscoe. The ethanolic extracts of BJK, its five individual plants, and pure constituents of BJK were investigated for their anti-allergic activity using immunoglobulin E (IgE)-sensitized β-hexosaminidase in the rat basophilic leukemia-2H3 (RBL-2H3) cells and anti-inflammatory activity using lipopolysaccharide (LPS)-induced nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) in the murine macrophage (RAW 264...
May 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28290212/spirodela-polyrhiza-extract-modulates-the-activation-of-atopic-dermatitis-related-ion-channels-orai1-and-trpv3-and-inhibits-mast-cell-degranulation
#17
Joo Hyun Nam, Hyo Won Jung, Young-Won Chin, Won-Mo Yang, Hyo Sang Bae, Woo Kyung Kim
CONTEXT: Spirodela polyrhiza (L.) Schleid. (Lemnaceae), Spirodelae Herba (SH), has been known to relieve inflammation, urticaria and skin symptoms including pruritus, eczema and rash. OBJECTIVE: The effects of SH extract on two calcium ion channels, Orai1 and TRPV3, and their potential as novel therapeutics for atopic dermatitis (AD) were investigated. The regulatory role of Orai1 on mast cell degranulation was evaluated. MATERIALS AND METHODS: The dried leaves of SH were extracted by 70% methanol...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28284550/adhesion-of-cdte-quantum-dots-on-model-membranes-and-internalization-into-rbl-2h3-cells
#18
Mengmeng Zhang, Xiaoran Wei, Lei Ding, Jingtian Hu, Wei Jiang
Quantum dots (QDs) have attracted broad attention due to their special optical properties and promising prospect in medical and biological applications. However, the process of QDs on cell membrane is worth further investigations because such process may lead to harmful effects on organisms and also important for QD application. In this study, adhesion of amino- and carboxyl-coated CdTe QDs (A-QDs and C-QDs) on cell membrane and the subsequent internalization are studied using a series of endocytosis-free model membranes, including giant and small unilamellar vesicles, supported lipid bilayers and giant plasma membrane vesicles (GPMVs)...
March 8, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28282470/phospholipid-scramblase-1-amplifies-anaphylactic-reactions-in-vivo
#19
Asma Kassas-Guediri, Julie Coudrat, Emeline Pacreau, Pierre Launay, Renato C Monteiro, Ulrich Blank, Nicolas Charles, Marc Benhamou
Mast cells are critical actors of hypersensitivity type I (allergic) reactions by the release of vasoactive and proinflammatory mediators following their activation by aggregation of the high-affinity receptor for immunoglobulin E (FcεRI). We have previously identified Phospholipid Scramblase 1 (PLSCR1) as a new molecular intermediate of FcεRI signaling that amplifies degranulation of the rat mast cell line RBL-2H3. Here we characterized primary mast cells from Plscr1-/- mice. The absence of PLSCR1 expression did not impact mast cell differentiation as evidenced by unaltered FcεRI expression, general morphology, amount of histamine stored and expression of FcεRI signal effector molecules...
2017: PloS One
https://www.readbyqxmd.com/read/28273137/adaptor-protein-3-a-key-player-in-rbl-2h3-mast-cell-mediator-release
#20
Elaine Zayas Marcelino da Silva, Edismauro Garcia Freitas-Filho, Devandir Antonio de Souza-Júnior, Luis Lamberti Pinto daSilva, Maria Celia Jamur, Constance Oliver
Mast cell (MC) secretory granules are Lysosome-Related Organelles (LROs) whose biogenesis is associated with the post-Golgi secretory and endocytic pathways in which the sorting of proteins destined for a specific organelle relies on the recognition of sorting signals by adaptor proteins that direct their incorporation into transport vesicles. The adaptor protein 3 (AP-3) complex mediates protein trafficking between the trans-Golgi network (TGN) and late endosomes, lysosomes, and LROs. AP-3 has a recognized role in LROs biogenesis and regulated secretion in several cell types, including many immune cells such as neutrophils, natural killer cells, and cytotoxic T lymphocytes...
2017: PloS One
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