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https://www.readbyqxmd.com/read/28549184/coiled-coil-interactions-mediate-serine-integrase-directionality
#1
Kushol Gupta, Robert Sharp, Jimmy B Yuan, Huiguang Li, Gregory D Van Duyne
Serine integrases are bacteriophage enzymes that carry out site-specific integration and excision of their viral genomes. The integration reaction is highly directional; recombination between the phage attachment site attP and the host attachment site attB to form the hybrid sites attL and attR is essentially irreversible. In a recent model, extended coiled-coil (CC) domains in the integrase subunits are proposed to interact in a way that favors the attPxattB reaction but inhibits the attLxattR reaction. Here, we show for the Listeria innocua integrase (LI Int) system that the CC domain promotes self-interaction in isolated Int and when Int is bound to attachment sites...
May 26, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28548595/effect-of-treatment-modality-on-in-hospital-outcome-in-patients-with-subarachnoid-hemorrhage-a-nationwide-study-in-japan-j-aspect-study
#2
Ryota Kurogi, Akiko Kada, Kunihiro Nishimura, Satoru Kamitani, Ataru Nishimura, Tetsuro Sayama, Jyoji Nakagawara, Kazunori Toyoda, Kuniaki Ogasawara, Junichi Ono, Yoshiaki Shiokawa, Toru Aruga, Shigeru Miyachi, Izumi Nagata, Shinya Matsuda, Shinichi Yoshimura, Kazuo Okuchi, Akifumi Suzuki, Fumiaki Nakamura, Daisuke Onozuka, Akihito Hagihara, Koji Iihara
OBJECTIVE Although heterogeneity in patient outcomes following subarachnoid hemorrhage (SAH) has been observed across different centers, the relative merits of clipping and coiling for SAH remain unknown. The authors sought to compare the patient outcomes between these therapeutic modalities using a large nationwide discharge database encompassing hospitals with different comprehensive stroke center (CSC) capabilities. METHODS They analyzed data from 5214 patients with SAH (clipping 3624, coiling 1590) who had been urgently hospitalized at 393 institutions in Japan in the period from April 2012 to March 2013...
May 26, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/28548473/initial-experience-with-the-e-ventus%C3%A2-stentgraft-for-endovascular-treatment-of-visceral-artery-aneurysms
#3
Susanne Anton, Erik Stahlberg, Marco Horn, Marcus Wiedner, Markus Kleemann, Joerg Barkhausen, Jan P Goltz
BACKGROUND: To evaluate the safety and efficacy of a novel balloon-expandable stentgraft for endovascular treatment of visceral artery aneurysms (VAA). METHODS: Between 9/2014 and 1/2017 seven patients (69+/-15y) with true (n=4) and false (n=3) VAAs were treated by implantation of balloon-expandable stentgrafts (E-ventus®, Jotec, Germany) using a trans-femoral (n=2) or -brachial (n=5) vascular access. The stentgraft was placed without prior passing of the landing zone with a sheath...
May 26, 2017: Journal of Cardiovascular Surgery
https://www.readbyqxmd.com/read/28547775/acidic-electrolyzed-water-delays-browning-by-destroying-conformation-of-polyphenoloxidase
#4
Jiangping Sun, Meng Wang, Haiquan Liu, Jing Xie, Yingjie Pan, Changhua Xu, Yong Zhao
BACKGROUND: Browning frequently occurs at fruits, vegetables and aquatic products during storage, it drastically reduces the consumer's acceptability and considerable financial loss. The objective of this paper was to investigate the effects of acidic electrolyzed water (AEW) technology on polyphenoloxidase (PPO), which is an essential enzyme for browning. RESULTS: AEW ice exhibited a good ability in delaying browning in shrimp. Kinetic study revealed that AEW exhibited the mixed type inhibition of PPO with Ki value of 1...
May 26, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28547183/performance-evaluation-of-a-high-pressure-microwave-assisted-flow-digestion-system-for-juice-and-milk-sample-preparation
#5
Thiago L Marques, Helmar Wiltsche, Joaquim A Nóbrega, Monika Winkler, Günter Knapp
Acid digestion is usually required for metal determination in food samples. However, this step is usually performed in batch mode which is time consuming, labor intensive, and may lead to sample contamination. Flow digestion can overcome these limitations. In this work, the performance of a high-pressure microwave-assisted flow digestion system with a large volume reactor was evaluated for liquid samples high in sugar and fat (fruit juice and milk). The digestions were carried out in a coiled perfluoroalkoxy (PFA) tube reactor (13...
May 26, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28547125/erratum-to-short-segment-coil-embolization-using-a-double-balloon-technique-in-an-experimental-vascular-model
#6
Daisuke Yunaiyama, Toru Saguchi, Tomohisa Moriya, Natsuhiko Shirota, Jun Otaka, Koichi Tokuuye, Yuichi Nagakawa, Akihiko Tsuchida, Kazuhiro Saito
No abstract text is available yet for this article.
May 25, 2017: Cardiovascular and Interventional Radiology
https://www.readbyqxmd.com/read/28546713/current-concepts-of-epigenetics-in-testicular-cancer
#7
REVIEW
Alfredo Harb-De la Rosa, Meenakkshy Manoharan, Ahmed Saeed Goolam
Testicular germ cell tumors (GCTs) are characterized into seminomas (SGCTs) and non-seminomatous testicular germ cell tumors (NSGCTs). Serum tumor markers (STMs) play an important role in testicular cancer as they provide useful information for diagnosis, staging, and detection of recurrence. Nonetheless, additional tumor markers for early diagnosis and therapeutic options are required to enhance specificity of serological diagnosis of testes cancers. Epigenetics is defined as inherited changes in gene expression that are not encoded in the DNA structure...
June 2017: Indian Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28546120/local-anesthesia-for-endovascular-treatment-of-unruptured-intracranial-aneurysms-feasibility-safety-and-periprocedural-complications
#8
Jihye Song
OBJECTIVE: The aim of the study is to report the feasibility, safety and outcomes associated with endovascular treatment (EVT) of unruptured intracranial aneurysms (UIA) under local anesthesia (LA). METHODS: Between March 2011 and December 2016, 184 consecutive patients with 198 UIA were treated with coil embolization under LA at the author's center. The data about medical comorbidities according to American Society of Anesthesiologists (ASA) grade, procedural details, and clinical and radiographic outcomes were reviewed...
May 22, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28545963/the-carboxy-terminal-region-of-the-tbc1d4-as160-rabgap-mediates-protein-homodimerization
#9
Ju Rang Woo, Soon-Jong Kim, Keon Young Kim, Hyonchol Jang, Steven E Shoelson, SangYoun Park
TBC1D4 (also known as AS160) is a Rab·GTPase-activating protein (RabGAP) which functions in insulin signaling. TBC1D4 is critical for translocation of glucose transporter 4 (GLUT4), from an inactive, intracellular, vesicle-bound site to the plasma membrane, where it promotes glucose entry into cells. The TBC1D4 protein is structurally subdivided into two N-terminal phosphotyrosine-binding (PTB) domains, a C-terminal catalytic RabGAP domain, and a disordered segment in between containing potential Akt phosphorylation sites...
May 22, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28545732/introduction-of-coiled-solid-phase-microextraction-fiber-coated-by-mesoporous-silica-cetyltrimethylammonium-bromide-for-ultra-trace-environmental-analysis
#10
Habib Razmi, Elnaz Marzi Khosrowshahi, Samaneh Farrokhzadeh
In this study, a tiny coiled cupper wire as a novel solid phase microextraction (SPME) fiber was coated with mesoporous silica/cetyltrimethylammonium bromide (MCM-41/CTAB) as an adsorbent by electrochemically assisted self-assembly method and used for the preconcentration of polycyclic aromatic hydrocarbons (PAHs) as model analytes prior to chromatographic determination. Deposition of MCM-41/CTAB on the coiled SPME (C-SPME) fiber resulted in easily controlled and reproducible SPME coatings. Non-calcined MCM-41/CTAB on C-SPME plays a key role in the adsorption of PAHs...
April 9, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28545128/common-variants-upstream-of-mlf1-at-3q25-and-within-cpz-at-4p16-associated-with-neuroblastoma
#11
Lee D McDaniel, Karina L Conkrite, Xiao Chang, Mario Capasso, Zalman Vaksman, Derek A Oldridge, Anna Zachariou, Millicent Horn, Maura Diamond, Cuiping Huo, Achille Iolascon, Hakon Hakonarson, Nazneen Rahman, Marcella Devoto, Sharon J Diskin
Neuroblastoma is a cancer of the developing sympathetic nervous system that most commonly presents in young children and accounts for approximately 12% of pediatric oncology deaths. Here, we report on a genome-wide association study (GWAS) in a discovery cohort or 2,101 cases and 4,202 controls of European ancestry. We identify two new association signals at 3q25 and 4p16 that replicated robustly in multiple independent cohorts comprising 1,163 cases and 4,396 controls (3q25: rs6441201 combined P = 1.2x10-11, Odds Ratio 1...
May 18, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28544979/preoperative-left-hepatic-lobe-devascularisation-to-minimize-perioperative-bleeding-in-a-jehovah-s-witness-undergoing-left-hepatectomy
#12
Laurence Weinberg, Georgina Hanus, Jonathan Banting, Diana Abu-Ssaydeh, Manfred Spanger, Su Kah Goh, Vijayaragavan Muralidharan
INTRODUCTION: Major liver resection in a Jehovah's Witness presents unique clinical challenges requiring multimodal blood minimization strategies to reduce perioperative complications. We report a case where complete left hepatic lobe devascularisation was undertaken to minimize bleeding in a Jehovah's Witness undergoing left hepatectomy. PRESENTATION OF CASE: A 65-year-old male Jehovah's Witness presented for open left hepatectomy for a large left-sided hepatocellular carcinoma involving segment IV of the liver...
May 15, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/28544160/a-unified-continuous-flow-assembly-line-synthesis-of-highly-substituted-pyrazoles-and-pyrazolines
#13
Joshua Britton, Timothy F Jamison
A rapid and modular continuous flow synthesis of highly functionalized fluorinated pyrazoles and pyrazolines has been developed. Flowing fluorinated amines through sequential reactor coils mediates diazoalkane formation and [3+2] cycloaddition to generate more than 30 azoles in a telescoped fashion. Pyrazole cores are then sequentially modified through additional reactor modules performing N-alkylation and arylation, deprotection, and amidation to install broad molecular diversity in short order. Continuous flow synthesis enables the safe handling of diazoalkanes at elevated temperatures, and the use of aryl alkyne dipolarphiles under catalyst free conditions...
May 25, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28544077/identification-and-analysis-of-5-enolpyruvylshikimate-3-phosphate-synthase-epsps-gene-from-glyphosate-resistant-ochrobactrum-intermedium-sq20
#14
Sadiqa Firdous, Samina Iqbal, Samina Anwar, Hina Jabeen
BACKGROUND: Glyphosate is a herbicide that acts by inhibition of the enzyme, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), entailed in catalysis of an essential step in the biosynthesis of aromatic amino acids. The objective of the current study was to isolate glyphosate resistant bacterial strains and subsequent characterization of the gene(s) encoding glyphosate resistance in these isolates. By enrichment culture technique, a glyphosate resistant bacterium, Ochrobactrum intermedium Sq20 was isolated from glyphosate contaminated indigenous soil and characterized...
May 25, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28543787/accurate-determination-of-brain-metabolite-concentrations-using-eretic-as-external-reference
#15
Niklaus Zoelch, Andreas Hock, Susanne Heinzer-Schweizer, Nikolai Avdievitch, Anke Henning
Magnetic Resonance Spectroscopy (MRS) can provide in vivo metabolite concentrations in standard concentration units if a reliable reference signal is available. For (1) H MRS in the human brain, typically the signal from the tissue water is used as the (internal) reference signal. However, a concentration determination based on the tissue water signal most often requires a reliable estimate of the water concentration present in the investigated tissue. Especially in clinically interesting cases, this estimation might be difficult...
May 25, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28543266/evaluation-of-pre-reconstruction-interpolation-methods-for-iterative-reconstruction-of-radial-k-space-data
#16
Ye Tian, K C Erb, Ganesh Adluru, Devavrat Likhite, Apoorva Pedgaonkar, Michael Blatt, Srikant Kamesh Iyer, John Roberts, Edward DiBella
PURPOSE: To evaluate the use of three different pre-reconstruction interpolation methods to convert non-Cartesian k-space data to Cartesian samples such that iterative reconstructions can be performed more simply and more rapidly. METHODS: Phantom as well as cardiac perfusion radial datasets were reconstructed by four different methods. Three of the methods used pre-reconstruction interpolation once followed by a fast Fourier transform (FFT) at each iteration. The methods were: bilinear interpolation of nearest-neighbor points (BINN), 3-point interpolation, and a multi-coil interpolator called GRAPPA Operator Gridding (GROG)...
May 19, 2017: Medical Physics
https://www.readbyqxmd.com/read/28542518/rnf41-interacts-with-the-vps52-subunit-of-the-garp-and-earp-complexes
#17
Delphine Masschaele, Leentje De Ceuninck, Joris Wauman, Dieter Defever, Frank Stenner, Sam Lievens, Frank Peelman, Jan Tavernier
RNF41 (Ring Finger Protein 41) is an E3 ubiquitin ligase involved in the intracellular sorting and function of a diverse set of substrates. Next to BRUCE and Parkin, RNF41 can directly ubiquitinate ErbB3, IL-3, EPO and RARα receptors or downstream signaling molecules such as Myd88, TBK1 and USP8. In this way it can regulate receptor signaling and routing. To further elucidate the molecular mechanism behind the role of RNF41 in intracellular transport we performed an Array MAPPIT (Mammalian Protein-Protein Interaction Trap) screen using an extensive set of proteins derived from the human ORFeome collection...
2017: PloS One
https://www.readbyqxmd.com/read/28542501/a-rho-associated-coiled-coil-containing-kinases-rock-inhibitor-y-27632-enhances-adhesion-viability-and-differentiation-of-human-term-placenta-derived-trophoblasts-in-vitro
#18
Kenichiro Motomura, Naoko Okada, Hideaki Morita, Mariko Hara, Masato Tamari, Keisuke Orimo, Go Matsuda, Ken-Ichi Imadome, Akio Matsuda, Takeshi Nagamatsu, Mikiya Fujieda, Haruhiko Sago, Hirohisa Saito, Kenji Matsumoto
Although human term placenta-derived primary cytotrophoblasts (pCTBs) represent a good human syncytiotrophoblast (STB) model, in vitro culture of pCTBs is not always easily accomplished. Y-27632, a specific inhibitor of Rho-associated coiled-coil containing kinases (ROCK), reportedly prevented apoptosis and improved cell-to-substrate adhesion and culture stability of dissociated cultured human embryonic stem cells and human corneal endothelial cells. The Rho kinase pathway regulates various kinds of cell behavior, some of which are involved in pCTB adhesion and differentiation...
2017: PloS One
https://www.readbyqxmd.com/read/28542017/children-with-single-sided-deafness-use-their-cochlear-implant
#19
Melissa Jane Polonenko, Blake Croll Papsin, Karen Ann Gordon
OBJECTIVES: To assess acceptance of a cochlear implant (CI) by children with single-sided deafness (SSD) as measured by duration of CI use across daily listening environments. DESIGN: Datalogs for 7 children aged 1.1 to 14.5 years (mean ± SD: 5.9 ± 5.9 years old), who had SSD and were implanted in their deaf ear, were anonymized and extracted from their CI processors. Data for all available follow-up clinical appointments were included, ranging from two to six visits...
May 24, 2017: Ear and Hearing
https://www.readbyqxmd.com/read/28541411/better-outcomes-and-reduced-hospitalization-cost-are-associated-with-ultra-early-treatment-of-ruptured-intracranial-aneurysms-a-us-nationwide-data-sample-study
#20
Ashish Sonig, Hussain Shallwani, Sabareesh K Natarajan, Hakeem J Shakir, L Nelson Hopkins, Kenneth V Snyder, Adnan H Siddiqui, Elad I Levy
BACKGROUND: The benefit of surgical treatment of ruptured aneurysms is well established. OBJECTIVE: To determine whether ultra-early ruptured aneurysm treatment leads to not only improved outcomes but also reduced hospitalization cost. METHODS: Using 2008-2011 Nationwide Inpatient Sample data, we analyzed demographic, clinical, and hospital factors for nontraumatic subarachnoid hemorrhage (SAH) patients who were "directly" admitted to the treating hospital where they underwent intervention (clipping/coiling)...
May 25, 2017: Neurosurgery
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