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https://www.readbyqxmd.com/read/28351016/nano-enabled-paper-humidity-sensor-for-mobile-based-point-of-care-lung-function-monitoring
#1
Mitradip Bhattacharjee, Harshal B Nemade, Dipankar Bandyopadhyay
The frequency of breathing and peak flow rate of exhaled air are necessary parameters to detect chronic obstructive pulmonary diseases (COPDs) such as asthma, bronchitis, or pneumonia. We developed a lung function monitoring point-of-care-testing device (LFM-POCT) consisting of mouthpiece, paper-based humidity sensor, micro-heater, and real-time monitoring unit. Fabrication of a mouthpiece of optimal length ensured that the exhaled air was focused on the humidity-sensor. The resistive relative humidity sensor was developed using a filter paper coated with nanoparticles, which could easily follow the frequency and peak flow rate of the human breathing...
March 22, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28345573/clinical-correlation-of-imaging-findings-in-congenital-cranial-dysinnervation-disorders-involving-abducens-nerve
#2
Chanchal Gupta, Pradeep Sharma, Rohit Saxena, Ajay Garg, Sanjay Sharma
PURPOSE: High-resolution magnetic resonance imaging (MRI) of intracranial parts of sixth nerve and seventh nerve and the extraocular muscles (EOMs) in orbit to correlate the clinical characteristics in patients with two special forms of strabismus in congenital cranial dysinnervation disorders which are Duane's retraction syndrome (DRS) and Mobius syndrome. MATERIALS AND METHODS: Morphological analysis by 3T MRI of orbit (using surface coils) and brain (using 32 channel head coil) was performed on 6 patients with clinical DRS (1 bilateral), 2 cases with Mobius syndrome, and 1 case with congenital sixth nerve palsy...
February 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28345248/application-of-thiol-yne-thiol-ene-reactions-for-peptide-and-protein-macrocyclizations
#3
Yuanxiang Wang, Benjamin Bruno, Sean Cornillie, Jason Nogueira, Diao Chen, Thomas Cheatham, Carol Lim, Danny Chou
We report the application of thiol-yne/thiol-ene reactions to synthesize mono- and bicyclic stapled peptides and proteins. We first developed a thiol-ene based peptide stapling method in aqueous conditions. This method enabled us to efficiently staple recombinantly expressed coil-coiled proteins. The resulting stapled protein demonstrates higher stability in its secondary structure than the unstapled version. Furthermore, we performed a thiol-yne coupling by using an α,ω-diyne to react with two cysteine residues to synthesize a stapled peptide with two vinyl sulfide groups...
March 26, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28345129/the-effect-of-microwave-on-the-interaction-of-flavor-compounds-with-g-actin-from-grass-carp-catenopharyngodon-idella
#4
Xiaowei Lou, Qiuli Yang, Yangying Sun, Daodong Pan, Jinxuan Cao
BACKGROUND: In order to investigate the influence of non-thermal effects of microwaves on the flavor of fish and meat products, the G-actin of grass carp in ice baths was exposed to different microwave powers (0, 100, 300 or 500 W); the surface hydrophobicity, sulphydryl contents, secondary structures and adsorption capacity of G-actin to ketones were determined. RESULTS: As microwave power increased from 0 to 300 W, the surface hydrophobicity, total and reactive sulphydryls increased; α-helix, β-sheet and random coil fractions turned into β-turn fractions...
March 26, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28343745/feasibility-of-sulfate-calcined-eggshells-for-removing-pathogenic-bacteria-and-antibiotic-resistance-genes-from-landfill-leachates
#5
Mao Ye, Mingming Sun, Xu Chen, Yanfang Feng, Jinzhong Wan, Kuan Liu, Da Tian, Manqiang Liu, Jun Wu, Arthur P Schwab, Xin Jiang
High abundance of human pathogen and antibiotic resistance genes (ARGs) in landfill leachate has become an emerging threat against human health. Therefore, sulfate- and calcination-modified eggshells as green agricultural bioresource were applied to test the feasibility of removing pathogenic bacteria and ARGs from leachate. The highest removal of Escherichia coli (E. coil) and gentamycin resistant gene (gmrA) from artificial contaminated landfill leachate was achieved by the application of eggshell with combined treatment of sulfate and calcination...
March 23, 2017: Waste Management
https://www.readbyqxmd.com/read/28342427/spectroscopic-studies-of-the-interaction-mechanisms-between-mono-caffeoylquinic-acids-and-transferrin
#6
Yanqing Guan, Jing Dong, Shizhong Chen, Meixian Liu, Daidong Wang, Xiaotian Zhang, Hong Wang, Zongtao Lin
Transferrin (Tf) is an important protein responsible for circulating and transporting iron into cytoplasm. Tf can be taken into cells through endocytosis mediated by Tf receptor, which usually overexpresses in cancer cells. The Tf-Tf receptor pathway opens a possible avenue for novel targeted cancer therapy by utilizing Tf-binding active compounds. Among which, anti-cancer active caffeoylquinic acids (CQAs) were recently found to be promising Tf-binders by our group. For better understanding the anti-cancer activities of CQAs, it is important to unveil the binding mechanisms between CQAs and Tf...
March 7, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28342211/the-molecular-behavior-of-a-single-%C3%AE-amyloid-inside-a-dipalmitoylphosphatidylcholine-bilayer-at-three-different-temperatures-an-atomistic-simulation-study
#7
Faezeh Kargar, Saeed Emadi, Hossein Fazli
The behavior of a single Aβ40 molecule within a dipalmitoylphosphatidylcholine (DPPC) bilayer was studied by all-atom molecular dynamics simulations. The effect of membrane structure was investigated on Aβ40 behavior, secondary structure and insertion depth. Simulations were performed at three temperatures (323, 310 and 300 K) to probe three different bilayer fluidities. Results show that at all above temperatures the peptide contains two short helices, coil, bend and turn structures. At 300 K, the peptide contains a region with β structure in C-terminal region...
March 25, 2017: Proteins
https://www.readbyqxmd.com/read/28340445/an-updated-meta-analysis-short-term-therapeutic-effects-of-repeated-transcranial-magnetic-stimulation-in-treating-obsessive-compulsive-disorder
#8
REVIEW
Dong-Dong Zhou, Wo Wang, Gao-Mao Wang, Da-Qi Li, Li Kuang
BACKGROUND: This study was conducted to evaluate the short-term therapeutic effects of using repeated transcranial magnetic stimulation (rTMS) to treat obsessive-compulsive disorder (OCD) and to examine potential influencing factors. METHOD: We searched the PubMed, EMBASE, CENTRAL, Wanfang, CNKI, and Sinomed databases on September 18, 2016 and reviewed the references of previous meta-analyses. Sham-controlled, randomized clinical trials using rTMS to treat OCD were included...
March 18, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/28339860/outcomes-with-single-coil-versus-dual-coil-implantable-cardioverter-defibrillators-a-meta-analysis
#9
Nicholas Sunderland, Amit Kaura, Francis Murgatroyd, Para Dhillon, Paul A Scott
Aims: Dual-coil implantable cardioverter defibrillator (ICD) leads have traditionally been used over single-coil leads due to concerns regarding high defibrillation thresholds (DFT) and consequent poor shock efficacy. However, accumulating evidence suggests that this position may be unfounded and that dual-coil leads may also be associated with higher complication rates during lead extraction. This meta-analysis collates data comparing dual- and single-coil ICD leads. Methods and results: Electronic databases were systematically searched for randomized controlled trials (RCT) and non-randomized studies comparing single-coil and dual-coil leads...
February 27, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28339790/does-systemic-administration-of-casein-phosphopeptides-affect-orthodontic-movement-and-root-resorption-in-rats
#10
Lachlan Crowther, Gang Shen, Mohammed Almuzian, Allan Jones, William Walsh, Rema Oliver, Peter Petocz, Nour E Tarraf, M Ali Darendeliler
Objectives: To assess the potential effects of casein phosphopeptides (CPPs) on orthodontically induced iatrogenic root resorption (OIIRR) and orthodontic teeth movement. Materials and methods: Forty Wistar rats (aged 11 weeks) were randomly divided into experimental group (EG; n = 20) that received a diet supplemented with CPP and control group (CG; n = 20) devoid of diet supplement. A 150 g force was applied using nickel titanium (NiTi) coil that was bonded on maxillary incisors and extended unilaterally to a maxillary first molar...
January 25, 2017: European Journal of Orthodontics
https://www.readbyqxmd.com/read/28339758/safety-and-efficacy-of-the-new-bidirectional-rotational-evolution%C3%A2-mechanical-lead-extraction-sheath-results-from-a-multicentre-italian-registry
#11
Patrizio Mazzone, Federico Migliore, Emanuele Bertaglia, Domenico Facchin, Elisabetta Daleffe, Vittorio Calzolari, Martino Crosato, Francesco Melillo, Francesco Peruzza, Alessandra Marzi, Nicoleta Sora, Paolo Della Bella
Aims: The aim of this prospective multicentre study is to evaluate safety and efficacy of the new bidirectional rotational mechanical lead extraction (LE) sheath (Evolution RL, Cook Medical, USA) in chronically implanted leads (>1-year-old leads). Methods and results: Between September 2013 and June 2016, a total of 238 leads in 124 consecutive patients were removed by using the new Evolution RL rotational mechanical sheath. Indications for LE were cardiac device infection in 63 (50...
February 23, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28339613/a-nonsense-mutation-in-ccdc62-gene-is-responsible-for-spermiogenesis-defects-and-male-infertility-in-repro29-repro29-mice%C3%A2
#12
Yuchi Li, Cailing Li, Shouren Lin, Bo Yang, Weiren Huang, Hanwei Wu, Yuanbin Chen, Lihua Yang, Manling Luo, Huan Guo, Jianbo Chen, Tiantian Wang, Qian Ma, Yanli Gu, Lisha Mou, Zhimao Jiang, Jun Xia, Yaoting Gui
Phenotype-driven mutagenesis is an unbiased method to identify novel genes involved in spermatogenesis and other reproductive processes. Male repro29/repro29 mice generated by the Reproductive Genomics Program at the Jackson Laboratory were infertile with deformed sperm and poor motility. Using selected exonic capture and massively parallel sequencing technologies, we identified a nonsense mutation in the exon 6 of coiled-coil domain-containing 62 gene (Ccdc62), which results in a formation of a premature stop codon and a truncated protein...
January 28, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28339580/the-integration-of-orthodontic-miniscrews-under-mechanical-loading-a-pre-clinical-study-in-rabbit
#13
B Giuliano Maino, Alberto Di Blasio, Davide Spadoni, Francesca Ravanetti, Carlo Galli, Antonio Cacchioli, Christos Katsaros, Mauro Gandolfini
Introduction: Orthodontic miniscrews are an increasingly popular choice to achieve absolute anchorage. The temporary use of miniscrews and their recent introduction have limited the debate over the biological aspect of the materials to that of the surface that permeates the field of dental implants. The aim of the present study was to investigate the integration of grade 5 titanium mini-implants with machined or sand blasted acid etched surface (SAE) under mechanical load in a rabbit tibia model of implant integration...
January 18, 2017: European Journal of Orthodontics
https://www.readbyqxmd.com/read/28339541/vitamin-d-suppresses-oxidative-stress-induced-microparticle-release-by-human-umbilical-vein-endothelial-cells
#14
Xiuyue Jia, Jie Xu, Yang Gu, Xin Gu, Weimin Li, Yuping Wang
Endothelial microparticle (MP) release was increased in numerous cardiovascular diseases including preeclampsia. Oxidative stress is a potent inducer of endothelial dysfunction. In this study, we aimed to investigate if vitamin D could protect endothelial cells (ECs) from MP release induced by oxidative stress. Endothelial cell (from human umbilical vein) oxidative stress was induced by cultivation of cells under lowered oxygen condition (2%O2) for 48 h and cells cultured under standard condition (21%O2) served as control...
December 27, 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/28338244/photoalignment-of-vertically-oriented-microphase-separated-lamellae-in-lc-lc-diblock-copolymer-thin-film
#15
Koichiro Beppu, Yuki Nagashima, Mituo Hara, Shusaku Nagano, Takahiro Seki
The microphase separation and photoalignment behavior of a diblock copolymer consisting of two kinds of side chain liquid crystalline (LC) polymers are investigated. Here, the mesogens of photoresponsive azobenzene and non-photoresponsive cyanobiphenyl are introduced in the side chains. The azobenzene and cyanobiphenyl mesogens orient homeotropically and planarly, respectively, in a thin film state. The combination of these contrasting orienting properties leads to a formation of vertically oriented lamella in the microphase separation structure...
March 24, 2017: Macromolecular Rapid Communications
https://www.readbyqxmd.com/read/28336771/dimerization-in-lbd16-and-lbd18-transcription-factors-is-critical-for-lateral-root-formation
#16
Han Woo Lee, Na Young Kang, Shashank Pandey, Chuloh Cho, Sung Haeng Lee, Jungmook Kim
LATERAL ORGAN BOUNDARIES (LOB) DOMAIN (LBD)/ASYMMETRIC LEAVES2-LIKE (ASL)s (hereafter referred to as LBD) are plant-specific transcription factors that play important roles in a plethora of plant growth and development. The leucine-zipper-like coiled-coil motif in the LOB domain of the class I LBD proteins has been proposed to mediate protein dimerization, but it has not been experimentally assessed yet. LBD16 and LBD18 have been well characterized to play important roles in lateral root development in Arabidopsis...
March 23, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28336587/draft-genome-sequence-of-propionibacterium-avidum-strain-ucd-pd2-isolated-from-a-feline-anal-sac
#17
Petra A Dahms, Alexandra L Martin, Holly H Ganz, Jonathan A Eisen, David A Coil
Here, we present the draft genome sequence of Propionibacterium (Cutibacterium) avidum strain UCD-PD2. The assembly contains 2,667,287 bp in 51 contigs. The strain was isolated from anal sac secretion samples collected from a feral domestic cat (Felis catus) as part of a larger project to study the microbiology of cats.
March 23, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28336437/overexpression-of-tacc3-is-correlated-with-tumor-aggressiveness-and-poor-prognosis-in-prostate-cancer
#18
Qiji Li, Liping Ye, Wei Guo, Min Wang, Shuai Huang, Xinsheng Peng
Transforming acidic coiled-coil (TACC3), a member of the TACC family, was previously reported to be deregulated in various cancers and identified to be involved in the tumor progression. However, its biological role and molecular mechanism in prostate cancer (PCa) have not been defined. Herein, we found that TACC3 was markedly upregulated in metastatic PCa. Upregulation of TACC3 was significantly associated with metastasis status, tumor stage, total PSA level, and Gleason score in PCa patients. Moreover, Kaplan-Meier survival analysis showed that PCa patients with high TACC3 had shorter disease-free survivals than those with low TACC3 expression...
March 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28336427/high-resolution-anatomical-and-quantitative-mri-of-the-entire-human-occipital-lobe-ex-vivo-at-9-4t
#19
S Sengupta, F J Fritz, R L Harms, S Hildebrand, D H Y Tse, B A Poser, R Goebel, A Roebroeck
Several magnetic resonance imaging (MRI) contrasts are sensitive to myelin content in gray matter in vivo which has ignited ambitions of MRI-based in vivo cortical histology. Ultra-high field (UHF) MRI, at fields of 7T and beyond, is crucial to provide the resolution and contrast needed to sample contrasts over the depth of the cortex and get closer to layer resolved imaging. Ex vivo MRI of human post mortem samples is an important stepping stone to investigate MRI contrast in the cortex, validate it against histology techniques applied in situ to the same tissue, and investigate the resolutions needed to translate ex vivo findings to in vivo UHF MRI...
March 20, 2017: NeuroImage
https://www.readbyqxmd.com/read/28336426/sar-simulations-safety
#20
Thomas M Fiedler, Mark E Ladd, Andreas K Bitz
At ultra-high fields, the assessment of radiofrequency (RF) safety presents several new challenges compared to low-field systems. Multi-channel RF transmit coils in combination with parallel transmit techniques produce time-dependent and spatially varying power loss densities in the tissue. Further, in ultra-high-field systems, localized field effects can be more pronounced due to a transition from the quasistationary to the electromagnetic field regime. Consequently, local information on the RF field is required for reliable RF safety assessment as well as for monitoring of RF exposure during MR examinations...
March 20, 2017: NeuroImage
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