Gabriel T Filsinger, Timothy M Wannier, Felix B Pedersen, Isaac D Lutz, Julie Zhang, Devon A Stork, Anik Debnath, Kevin Gozzi, Helene Kuchwara, Verena Volf, Stan Wang, Xavier Rios, Christopher J Gregg, Marc J Lajoie, Seth L Shipman, John Aach, Michael T Laub, George M Church
Efficient genome editing methods are essential for biotechnology and fundamental research. Homologous recombination (HR) is the most versatile method of genome editing, but techniques that rely on host RecA-mediated pathways are inefficient and laborious. Phage-encoded single-stranded DNA annealing proteins (SSAPs) improve HR 1,000-fold above endogenous levels. However, they are not broadly functional. Using Escherichia coli, Lactococcus lactis, Mycobacterium smegmatis, Lactobacillus rhamnosus and Caulobacter crescentus, we investigated the limited portability of SSAPs...
April 2021: Nature Chemical Biology