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Hplc pharmacokinetics

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https://www.readbyqxmd.com/read/28432941/validation-of-a-spe-hplc-uv-method-for-the-quantification-of-a-new-er-specific-photosensitizer-or-141-in-blood-serum-using-total-error-concept
#1
Emilie Bony, Yohan Mace, Adán Pinto, David Delvaux, Robert Kiss, Olivier Riant, Olivier Feron, Joëlle Quetin-Leclercq
The aim of this work is to develop the first validated HPLC-UV method quantification in blood serum for a new endoplasmic reticulum (ER)-specific benzophenazine photosensitizer (OR-141). A fast solid phase extraction (SPE) cleaning sample procedure was achieved on C18 encapped (ec) SPE cartridges and the separation was performed on a RP-18e column (5μM) using an isocratic elution with methanol. The method has been fully validated according to accuracy profiles based on total error and tolerance intervals. Calibration was performed in the matrix and trueness (<4...
April 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28419964/effects-of-paeonia-emodi-on-hepatic-cytochrome-p450-cyp3a2-and-cyp2c11-expression-and-pharmacokinetics-of-carbamazepine-in-rats
#2
Mohammad Raish, Ajaz Ahmad, Khalid M Alkharfy, Basit L Jan, Kazi Mohsin, Abdul Ahad, Fahad I Al-Jenoobi, Abdullah M Al-Mohizea
Herbal medicines, dietary supplements, and other foods may pharmacokinetically and/or pharmacodynamically interact with carbamazepine (CBZ), which could lead to potential clinical consequences. Paeonia emodi (PE) is one of the herbs used as complementary therapy in the treatment of epileptic patients in some cultures, and may also be co-administered with CBZ. This study evaluates the effects of PE on the pharmacokinetics of CBZ and determines a possible mechanism of interaction. Rats were administered vehicle saline or PE (200mg/kg, p...
April 15, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28412689/simple-rapid-and-highly-sensitive-hplc-method-for-measurement-of-lamotrigine-in-human-plasma-and-its-clinical-applications
#3
Abdul Sami Shaikh, Wenlong Li, Guiyan Yuan, Meimei Gao, Chunmei Geng, Nan Guo, Ruichen Guo
Spikes in Lamotrigine concentrations levels and associated clinical toxicity may occur unpredictably. This study describes the development and validation of a simple, more rapid, highly sensitive and economical method for measuring Lamotrigine (LTG) concentration levels in human plasma using HPLC-UV and its clinical applications. Analyte from plasma was extracted with methanol (protein precipitation) and separated on the analytical column Diamonsil C18 (150mm×4.6mm, 5μm) Waters-Milford, MA, United States...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28411632/an-ultra-specific-and-sensitive-sandwich-elisa-for-imatinib-using-two-anti-imatinib-antibodies
#4
Tetsuya Saita, Yuta Yamamoto, Kazuhisa Hosoya, Yutaro Yamamoto, Sakiko Kimura, Yutaka Narisawa, Masashi Shin
The development of an immunoassay for a low-molecular-weight drug first requires the identification of specific antibodies that do not cross-react with the drug's metabolites. If two antibodies can simultaneously recognize the entire structure of the drug, we can then utilize them to establish an ultra-specific sandwich ELISA, free from interference due to the metabolic products of the drug. This paper reports an ultra-specific and sensitive sandwich ELISA for determination of the tyrosine kinase inhibitor imatinib using two anti-imatinib antibodies...
May 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28408532/-68-ga-thp-psma-a-pet-imaging-agent-for-prostate-cancer-offering-rapid-room-temperature-one-step-kit-based-radiolabeling
#5
Jennifer D Young, Vincenzo Abbate, Cinzia Imberti, Levente K Meszaros, Michelle T Ma, Samantha Y A Terry, Robert C Hider, Greg E Mullen, Philip J Blower
The clinical impact and accessibility of (68)Ga tracers for the prostate-specific membrane antigen (PSMA) and other targets would be greatly enhanced by the availability of a simple, one-step kit-based labeling process. Radiopharmacy staff are accustomed to such procedures in the daily preparation of (99m)Tc radiopharmaceuticals. Currently, chelating agents used in (68)Ga radiopharmaceuticals do not meet this ideal. AIM: To develop and evaluate preclinically a (68)Ga radiotracer for imaging PSMA expression that could be radiolabeled simply by addition of (68)Ga generator eluate to a cold kit...
April 13, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28407418/pharmacokinetics-and-acid-suppressant-efficacy-of-esomeprazole-after-intravenous-oral-and-subcutaneous-administration-to-healthy-beagle-dogs
#6
J-H Hwang, J-W Jeong, G-H Song, T-S Koo, K-W Seo
BACKGROUND: Esomeprazole is an S-enantiomer of omeprazole that has favorable pharmacokinetics and efficacious acid suppressant properties in humans. However, the pharmacokinetics and effects on intragastric pH of esomeprazole in dogs have not been reported. OBJECTIVE: To determine the pharmacokinetics of esomeprazole administered via various routes (PK study) and to investigate the effect of esomeprazole on intragastric pH with a Bravo pH monitoring system (PD study)...
April 13, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28404688/brain-disposition-of-cis-para-methyl-4-methylaminorex-cis-4-4-dmar-and-its-potential-metabolites-after-acute-and-chronic-treatment-in-rats-correlation-with-central-behavioral-effects
#7
Jacopo Lucchetti, Claudio Marcello Marzo, Alice Passoni, Angelo Di Clemente, Federico Moro, Renzo Bagnati, Marco Gobbi, Luigi Cervo
Para-methyl-4-methylaminorex (4,4'-DMAR) is a phenethylamine derivative with psychostimulant activity, whose abuse has been associated with several deaths and a wide range of adverse effects. We recently validated an HPLC-MS/MS method to measure the compound's concentrations in plasma, and applied it to describe the pharmacokinetic properties after single dose in rats. In this study we investigated the brain disposition and metabolism of cis-4,4'-DMAR after intraperitoneal injection, and its central behavioral effects...
April 12, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/28393405/determination-of-irinotecan-sn-38-and-sn-38-glucuronide-using-hplc-ms-ms-application-in-a-clinical-pharmacokinetic-and-personalized-medicine-in-colorectal-cancer-patients
#8
Chalirmporn Atasilp, Pichai Chansriwong, Ekapob Sirachainan, Thanyanan Reungwetwattana, Apichaya Puangpetch, Santirhat Prommas, Suwannee Sirilerttrakul, Budsaba Rerkarmnuaychoke, Sansanee Wongwaisayawan, Chonlaphat Sukasem
BACKGROUND: Irinotecan (CPT-11) is chemotherapy used mainly in the metastatic colorectal cancer. The purpose of this study was to develop and validate the LC-MS/MS for the simultaneous determination of CPT-11, SN-38, and SN-38G. METHODS: A 100 μL of plasma was prepared after protein precipitation and analyzed on a C18 column using 0.1% acetic acid in water and 0.1% acetic acid in acetonitrile as mobile phases. The mass spectrometer worked with multiple reaction monitoring (MRM) in positive scan mode...
April 10, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28385011/pharmacokinetics-of-tilmicosin-in-healthy-pigs-and-in-pigs-experimentally-infected-with-haemophilus-parasuis
#9
Ling Zhang, Li Zhao, Yonghong Liu, Junfeng Liu, Xianqiang Li
A comparative in vivo pharmacokinetic (PK) study of tilmicosin (TIL) was conducted in 6 crossbred healthy pigs and 6 crossbred pigs infected with Haemophilus parasuis (H. parasuis) following oral administration of a single dose of 40 mg/kg. The infected model was established by intranasal inoculation and confirmed by clinical signs, blood biochemical and microscopic examination. TIL concentrations in plasma were determined by a validated high-performance liquid chromatography (HPLC) method with ultra violet detection at a wavelength of 285 nm and PK parameters were calculated by using the Winnonlin software...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28378572/effect-of-compatible-herbs-on-the-pharmacokinetics-of-effective-components-of-panax-notoginseng-in-fufang-xueshuantong-capsule
#10
Huan-Huan Pang, Meng-Yi Li, Yuan Wang, Min-Ke Tang, Chang-Hua Ma, Jian-Mei Huang
Fufang Xueshuantong (FXT) is a well-known Chinese herbal formula which has been used to treat cardiovascular and ophthalmic diseases, especially diabetic retinopathy. Panax notoginseng (Burkill) F.H. Chen (PN) is the main herb of FXT, whose major bioactive constituents are ginsenosides. However, the scientific basis of the compatibility of FXT is still ambiguous. The present study investigated the scientific basis of the compatibility of FXT by comparing the pharmacokinetics of marker compounds after oral administrations of PN and FXT...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28375127/pharmacokinetic-evaluation-of-anticancer-drugs-in-hodgkin-s-lymphoma-patients-after-their-simultaneous-administration
#11
Muhammad Zubair Malik, Mahmood Ahmad, Saleh Muahammad, Muhammad Naeem Aamir
A pharmacokinetic study of anticancer drugs was carried out in 18 Hodgkin's lymphoma male patients. The anticancer drugs were administered to the patient by a standard procedure and a validated HPLC method was used for plasma concentration determination. Maximum plasma concentration (Cmax) of Adriamycin, Bleomycin, Vinblastine and Dacarbazine (ABVD) were 7.71, 4.32, 7.95 and 6.51µg/ml respectively. Adriamycin and Dacarbazine exhibited longer Tmax compared to Bleomycin and Vinblastine. Area under the curve values of ABVD were 118...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28373891/simultaneous-determination-and-pharmacokinetic-study-of-quercetin-luteolin-and-apigenin-in-rat-plasma-after-oral-administration-of-matricaria-chamomilla-l-extract-by-hplc-uv
#12
Xiaoxv Dong, Wei Lan, Xingbin Yin, Chunjing Yang, Wenping Wang, Jian Ni
A simple and sensitive HPLC-UV method has been developed for the simultaneous determination of quercetin, luteolin, and apigenin in rat plasma after oral administration of Matricaria chamomilla L. extract. The flow rate was set at 1.0 ml/min and the detection wavelength was kept at 350 nm. The calibration curves were linear in the range of 0.11-11.36 μg/ml for quercetin, 0.11-11.20 μg/ml for luteolin, and 0.11-10.60 μg/ml for apigenin, respectively. The intraday and interday precisions (RSD) were less than 8...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28370390/auto-inhibitory-properties-of-the-parent-but-not-of-the-n-oxide-metabolite-contribute-to-infusion-rate-dependent-voriconazole-pharmacokinetics
#13
Nicolas Hohmann, Rebecca Kreuter, Antje Blank, Johanna Weiss, Jürgen Burhenne, Walter E Haefeli, Gerd Mikus
PURPOSE: The pharmacokinetics of voriconazole show a non-linear dose-exposure relationship caused by inhibition of its own CYP3A-dependent metabolism. Because the magnitude of auto-inhibition also depends on voriconazole concentrations, infusion rate might modulate voriconazole exposure. The impact of four different infusion rates on voriconazole pharmacokinetics was investigated. METHODS: Twelve healthy participants received 100 mg voriconazole i.v. over 4 h, 400 mg over 6 h, 4 h, and 2 h in a crossover design...
March 29, 2017: British Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/28366660/pharmacokinetics-and-cerebrospinal-fluid-penetration-of-norvancomycin-in-chinese-adult-patients
#14
Jun Li, Shengquan He, Zhiping Yang, Congxia Lu
Norvancomycin is an antibiotic that has been approved for the treatment of infections caused by antibiotic-resistant Gram-positive bacteria and has been used in China for more than a decade. However, the cerebrospinal fluid (CSF) penetration of norvancomycin has not been evaluated. The aims of the study were (i) to investigate the pharmacokinetics and CSF penetration of norvancomycin in meningitis and non-meningitis patients and (ii) to recommend favourable dosing regimens in meningitis patients. Twenty adult patients (ten with meningitis and ten without meningitis) requiring norvancomycin treatment were enrolled...
March 30, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28364662/logp-a-yesterday-s-value
#15
Chrysoula Vraka, Lukas Nics, Karl-Heinz Wagner, Marcus Hacker, Wolfgang Wadsak, Markus Mitterhauser
INTRODUCTION: There is an increasing demand for high throughput methods at early stages of preclinical radioligand development, in order to predict pharmacokinetic properties (e.g., biodistribution) and blood brain barrier (BBB) penetration. One of the most important physicochemical properties is the lipophilicity, measured by means of shake-flask (logP) or HPLC methods. Yet, a plethora of experimental methods are described in the literature for the determination of logP values. These varying methods often lead to different results for one identical compound, which complicates any comparison or prediction for subsequent preclinical studies...
March 21, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/28364250/ultrasensitive-hybridization-based-elisa-method-for-the-determination-of-phosphorodiamidate-morpholino-oligonucleotides-in-biological-samples
#16
Umar Burki, Volker Straub
Determining the concentration of oligonucleotide in biological samples such as tissue lysate and serum is essential for determining the biodistribution and pharmacokinetic profile, respectively. ELISA-based assays have shown far greater sensitivities compared to other methods such as HPLC and LC/MS. Here, we describe a novel ultrasensitive hybridization-based ELISA method for quantitating morpholino oligonucleotides in mouse tissue lysate and serum samples. The assay has a linear detection range of 5-250 pM (R2 > 0...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28359984/a-selective-hplc-ms-ms-method-for-quantification-of-snd-117-in-rat-plasma-and-its-application-to-a-pharmacokinetic-study
#17
Bei-Hua Bao, An Kang, Yang Zhao, Qi Shen, Jun-Song Li, Liu-Qing Di, Jian-Xin Li
Rheumatoid arthritis (RA), a chronic systemic inflammatory disorder affects many adults. Sinomenine, a natural product, has been clinically available for the treatment of RA in China. SND-117, a sinomenine derivative with much more potent activity, might serve as a candidate for anti-arthritis. The aim of the present study was to develop a sensitive and rapid high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method for quantification of SND-117 in rat plasma and to understand its absolute bioavailability...
March 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28359233/in-vivo-hypoglycemic-studies-of-polyherbal-phytoceuticals-their-pharmacokinetic-studies-and-dose-extrapolation-by-allometric-scaling
#18
Baishakhi De, Koushik Bhandari, Prakash Katakam, Analava Mitra
BACKGROUND: This work reports the safety profiling, in vivo hypoglycemic and pharmacokinetic studies of three phytoceuticals viz. conventional and sustained release tablets and microspheres each containing a polyherbal product phytocomposite (PHC) as the active ingredient. PHC is prepared from the leaf extracts of Ficus benghalensis: Syzigium cumini: Ocimum sanctum mixed in the weight ratio of 1:1:2. Further no observed adverse effect level (NOAEL), maximum recommended starting dose (MRSD) in human and prediction of human pharmacokinetic parameters have been accomplished by allometric equations...
March 29, 2017: Current Drug Discovery Technologies
https://www.readbyqxmd.com/read/28346882/determination-of-prodrug-treosulfan-and-its-biologically-active-monoepoxide-in-rat-plasma-liver-lungs-kidneys-muscle-and-brain-by-hplc-esi-ms-ms-method
#19
Michał Romański, Anna Kasprzyk, Artur Teżyk, Agnieszka Widerowska, Czesław Żaba, Franciszek Główka
A prodrug treosulfan (TREO) is currently investigated in clinical trials for conditioning prior to hematopoietic stem cell transplantation. Bioanalysis of TREO and its active derivatives, monoepoxide (S,S-EBDM) and diepoxide, in plasma and urine underlay the pharmacokinetic studies of these compounds but cannot explain an organ pharmacological action or toxicity. Recently, distribution of TREO and S,S-EBDM into brain, cerebrospinal fluid, and aqueous humor of the eye has been investigated in animal models and the obtained results presented clinical relevance...
June 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28346059/deferasirox-pharmacogenetic-influence-on-pharmacokinetic-efficacy-and-toxicity-in-a-cohort-of-pediatric-patients
#20
Sarah Allegra, Silvia De Francia, Jessica Cusato, Arianna Arduino, Davide Massano, Filomena Longo, Antonio Piga, Antonio D'Avolio
AIM: We aimed to evaluate the influence of genetic polymorphisms involved in deferasirox metabolism and transport on its pharmacokinetics and treatment toxicity, in a cohort of β-thalassaemic children. PATIENTS & METHODS: Drug plasma concentrations were measured by a HPLC-UV method. Allelic discrimination for UGT1A1, UGT1A3, CYP1A1, CYP1A2, CYP2D6, MRP2 and BCRP1 polymorphisms was performed by real-time PCR. RESULTS: CYP1A1 rs2606345AA influenced Ctrough (p = 0...
March 27, 2017: Pharmacogenomics
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