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Hplc pharmacokinetics

Reza Naeimi, Fatemeh Safarpour, Mona Hashemian, Hamed Tashakorian, Seyed Raheleh Ahmadian, Manouchehr Ashrafpour, Maryam Ghasemi-Kasman
Curcumin has been introduced as effective anti-inflammatory agent in treatment of several inflammatory disorders. Despite the wide range pharmacological activities, clinical application of curcumin is restricted mainly due to the low water solubility of this substance. More recently, we could remarkably improve the aqueous solubility of curcumin by its encapsulation in chitosan-alginate-sodium tripolyphosphate nanoparticles (CS-ALG-STPP NPs). In this study, the anti-inflammatory and myelin protective effects of curcumin-loaded NPs were evaluated in lysolecithin (LPC)-induced focal demyelination model...
March 9, 2018: Neuroscience Letters
Shuyu Zhan, Baoyue Ding, Yu-Er Ruan, Xuan Huang, Guoqiang Liu, Xiaoqing Lv, Xiong Huang, Mingjuan Li, Ninghua Jiang, Qing Shao
Microdialysis is a powerful in vivo sampling technique for pharmacokinetic-pharmacodynamic (PK-PD) modeling of drugs in pre-clinical and clinical studies. However, the noticeable limitations of previous studies using microdialysis were that animals anesthesia in the whole experiment and the combination of microdialysis and blood sampling for drug and (or) effect detection, which can obviously influence PK and PD behavior of drugs. In this study, a simple blood microdialysis sampling system in freely-moving rats was established for simultaneous study of PK and PD of Shengmai injection (SMI) effect on inducing real-time nitric oxide (NO) release on isoproterenol (ISO) induced myocardial ischemia rats...
March 9, 2018: Journal of Pharmaceutical and Biomedical Analysis
Marycarmen Cruz-Hurtado, Ma de Lourdes López-González, Derly Constanza Escobar-Wilches, Adolfo Sierra-Santoyo
Vinclozolin (V) is a fungicide with anti-androgenic properties whose metabolism is not fully understood, and data on urinary elimination of either V or its metabolites are limited. Therefore the kinetics of urinary elimination of V and its metabolites, after an oral dose in adult male rats were investigated. A single oral dose of V (100 mg/kg) suspended in corn oil was administered to male adult Wistar rats, and urine was collected at different times after dosing. V and its metabolites were extracted from urine, then enzymatically hydrolyzed using β-glucuronidase/sulfatase of H...
March 8, 2018: Toxicology and Applied Pharmacology
Ailing Hui, Huayang Yin, Zheng Zhang, An Zhou, Jingchao Chen, Li Yang, Zeyu Wu, Wencheng Zhang
Tetramethylpyrazine was introduced to the structure of danshensu (DSS) as P-glycoprotein (P-gp)-inhibiting carrier, designing some novel brain-targeting DSS-pyrazine derivatives via prodrug delivery strategy. Following the virtual screening, three DSS-pyrazine esters (DT1, DT2, DT3) were selected because of their better prediction parameters related to brain-targeting. Among them, DT3 was thought to be a promising candidate due to its appropriate bioreversible property in vitro release assay. Further investigation with regard to DT3's brain-targeting effects in vivo was also reported in this study...
March 9, 2018: Drug Delivery and Translational Research
Jing Zhan, Ya Ding, Benyan Zou, Hai Liao, Wenqi Jiang, Su Li
BACKGROUND AND OBJECTIVE: Puquitinib mesylate (XC-302) is a new multiple-target anticancer inhibitor, which directly suppresses the activity of phosphatidylinositol 3-kinase (PI3K). This study was aimed to develop a sensitive and specific liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI MS/MS) method for the quantification and pharmacokinetic investigation of plasma puquitinib in cancer patients. METHODS: The analytes of human plasma were prepared by liquid-liquid extraction using methyl-t-butyl ether (MTBE)...
March 8, 2018: European Journal of Drug Metabolism and Pharmacokinetics
A J Cerreta, G A Lewbart, D R Dise, M G Papich
Ceftazidime, a third-generation cephalosporin, is important for treating opportunistic bacterial infections in turtles. Antibacterial dosage regimens are not well established for wild turtles and are often extrapolated from other reptiles or mammals. This investigation used a population pharmacokinetic approach to study ceftazidime in wild turtles presented for rehabilitation. Ceftazidime was administered to 24 wild turtles presented to the Turtle Rescue Team at North Carolina State University. A sparse blood sampling protocol was used to collect samples from 0 to 120 hr with three samples per individual after injection...
March 8, 2018: Journal of Veterinary Pharmacology and Therapeutics
Mi-Jung Kim, Ji-Yeong Byeon, Young-Hoon Kim, Se-Hyung Kim, Choong-Min Lee, Eui Hyun Jung, Won Ki Chae, Yun Jeong Lee, Choon-Gon Jang, Seok-Yong Lee, Chang-Ik Choi
Clomiphene citrate, a selective estrogen receptor modulator, is metabolized into its 4-hydroxylated active metabolites, primarily by CYP2D6. In this study, we investigated the effects of the most common CYP2D6 variant allele in Asians, CYP2D6*10, on the pharmacokinetics of clomiphene and its two active metabolites (4-OH-CLO and 4-OH-DE-CLO) in healthy Korean subjects. A single 50-mg oral dose of clomiphene citrate was given to 22 Korean subjects divided into three genotype groups according to CYP2D6 genotypes, CYP2D6*wt/*wt (n = 8; *wt = *1 or *2), CYP2D6*wt/*10 (n = 8) and CYP2D6*10/*10 (n = 6)...
March 7, 2018: Archives of Pharmacal Research
Agnieszka Karbownik, Wojciech Polom, Joanna Porazka, Edyta Szalek, Tomasz Grabowski, Anna Wolc, Matuszewski Marcin, Edmund Grzesowiak
Paracetamol is one of the most common analgesic and antipyretic drugs. Recently intravenous paracetamol has been widely used to treat moderate postoperative pain. Surgery is the main method of treatment of renal cancer. Total or partial nephrectomy can be performed, depending on the size and location of the tumor. Pharmacokinetics of drugs may depend on the type of surgery. The aim of the study was to compare the postinfusion pharmacokinetics of paracetamol in patients after total nephrectomy (TN) and nephron sparing surgery (NSS)...
May 2017: Acta Poloniae Pharmaceutica
Y Hai-Feng, Z Chuan-Gang, L I Yong-Jun, L U Ke, L I Hao, S Yao-Jun, Z Ao-Bo, L U Guang-Fu, B U Shi-Jin
Objective To investigate the pharmacokinetics and relative bioavailability of praziquantel injection in buffaloes in contrast to praziquantel tablet. Methods A single oral administration of praziquantel tablet at a dose of 20 mg/kg or intramuscular administration of praziquantel injection at a dose of 10 mg/kg was performed in six healthy adult buffalos according to a twoperiod crossover design. The praziquantel concentration in plasma was determined by a high performance liquid chromatography (HPLC) method...
July 28, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
Yongzhi Tian, Xiao Wu, Mingjie Zhang, Longshan Zhao, Zhili Xiong, Feng Qin
A rapids, selective and sensitive high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed to determine meloxicam in beagle dog plasma. Sample pretreatment involved a one-step protein precipitation with methanol of 0.1 mL plasma. Analysis was performed on a Venusil ASB-C18 column with mobile phase consisting of methanol-water (containing 0.1% formic acid) (75:25, v/v). The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source...
March 5, 2018: Biomedical Chromatography: BMC
Dalia Mohamed, Maha A Hegazy, Mona S Elshahed, Safaa S Toubar, Marwa I Helmy
A facile, fast and specific method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the simultaneous quantitation of paracetamol (PAR), chlorzoxazone (CXZ) and aceclofenac (ACL) in human plasma was developed and validated. Sample preparation was achieved by liquid-liquid extraction. The analysis was performed on a reversed-phase C18 HPLC column (5 μm, 4.6× 50 mm) using acetonitrile: 10 mM ammonium formate pH 3.0 (65:35, v/v) as the mobile phase where atrovastatin (ATR) was used as an internal standard...
March 5, 2018: Biomedical Chromatography: BMC
Eszter Boros, Omar R Pinkhasov, Peter Caravan
Background: Current analytical methods for characterizing pharmacokinetic and metabolic properties of positron emission tomography (PET) and single photon emission computed tomography (SPECT) probes are limited. Alternative methods to study tracer metabolism are needed. The study objective was to assess the potential of high performance liquid chromatography - inductively coupled plasma - mass spectrometry (HPLC-ICP-MS) for quantification of molecular probe metabolism and pharmacokinetics using stable isotopes...
2018: EJNMMI Radiopharm Chem
Michał Romański, Franciszek Główka
Currently, there is an urgent need to establish the optimal dosing of TREO in conditioning prior to hematopoietic stem cell transplantation, especially in children. For that purpose, pharmacokinetic analyses are ongoing within clinical phase II and III trials. In this paper, HPLC methods for determination of prodrug treosulfan and/or its biologically active epoxides in human plasma or serum are reviewed for the first time, including the spectrum of analytes being quantified, detection type, and derivatization methodology...
February 24, 2018: Journal of Pharmaceutical and Biomedical Analysis
Zhi-Feng Li, Meng-Ying Zhou, Ting Tan, Chen-Cong Zhong, Qi Wang, Ling-Ling Pan, Ying-Ying Luo, Shi-Lin Yang, Yu-Lin Feng, Hui Ouyang
Ziyuglycoside I (ZGS1) is a promising drug candidate for the treatment of leucopenia. Currently, information on ZGS1 and its in vivo metabolite ziyuglycoside II (ZGS2) is limited. The objective of this study was to investigate the pharmacokinetics, tissue distribution, and excretion of ziyuglycoside I (ZGS1) and its metabolite ziyuglycoside II (ZGS2) in rats. In our study, a simple and sensitive high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) method was established for simultaneous determination of ZGS1 and its metabolite for Sprague-Dawley rat pharmacokinetics studies...
February 28, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Fawzy Elbarbry, Aimy Ung, Khaled Abdelkawy
Background: Quercetin (QR) and thymoquinone (TQ) are herbal remedies that are currently extensively used by the general population to prevent and treat various chronic conditions. Therefore, investigating the potential of pharmacokinetic interactions caused by the concomitant use of these herbal remedies and conventional medicine is warranted to ensure patient safety. Purpose of the Study: This study was conducted to determine the inhibitory effect of QR and TQ, two commonly used remedies, on the activities of selected cytochrome P450 (CYP) enzymes that play an important role in drug metabolism and/or toxicology...
January 2018: Pharmacognosy Magazine
Benedicte Lelievre, Marie Briet, Charlotte Godon, Pierre Legras, Jeremie Riou, Patrick Vandeputte, Bertrand Diquet, Jean Philippe Bouchara
Cerebral Scedosporium infections usually occur in lung transplant recipients as well as in immunocompetent patients in the context of near-drowning. Voriconazole is the first-line treatment. The diffusion of voriconazole through the blood-brain barriers in the context of cerebral infection and cyclosporine administration is crucial and remains a matter of debate. To address this issue, the pharmacokinetics of voriconazole were assessed in the plasma, cerebrospinal fluid (CSF), and brain, in an experimental model of cerebral scedosporiosis in rats receiving or not cyclosporine...
February 28, 2018: Journal of Pharmacology and Experimental Therapeutics
Gaurav Kumar, Pankaj Paliwal, Sumedha Mukherjee, Nishant Patnaik, Sairam Krishnamurthy, Ranjana Patnaik
The present study is designed to investigate the brain distribution and plasma pharmacokinetics profiles of chlorogenic acid after intranasal administration in Charles-Foster rats to evaluate whether the CGA molecules are transported directly via the nose-to-brain path. The chlorogenic acid is administered intravenously (IV) and intranasally (IN) at the dose of 10 mg/kg. Further, its concentration in the plasma, cerebrospinal fluid (CSF) and the whole brain is analyzed by HPLC-UV method. The study observes that chlorogenic acid is rapidly absorbed in plasma with tmax of 1 min similar to IV route after IN administration...
February 24, 2018: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
Shu Yao, Li Li, Xuan-Tao Su, Kai Wang, Zai-Jun Lu, Cun-Zhong Yuan, Jin-Bo Feng, Shi Yan, Bei-Hua Kong, Kun Song
BACKGROUND: Ovarian cancer is the most leading cause of death and the third most common gynecologic malignancy in women. Traditional chemotherapy has inevitable drawbacks of nonspecific tumor targeting, high toxicity, and poor therapeutic efficiency. In order to overcome such shortcomings, we prepared a novel nano-carrier drug-delivery system to enhance the anti-tumor efficiency. METHODS: In vitro characterizations of nano-carriers were determined by TEM, DLS. Cell viability was measured by MTT method...
February 26, 2018: Journal of Experimental & Clinical Cancer Research: CR
Bianca Posocco, Mauro Buzzo, Andrea Follegot, Luciana Giodini, Roberto Sorio, Elena Marangon, Giuseppe Toffoli
Paclitaxel belongs to the taxanes family and it is used, alone or in multidrug regimens, for the therapy of several solid tumours, such as breast-, lung-, head and neck-, and ovarian cancer. Standard dosing of chemotherapy does not take into account the many inter-patient differences that make drug exposure highly variable, thus leading to the insurgence of severe toxicity. This is particularly true for paclitaxel considering that a relationship between haematological toxicity and plasma exposure was found...
2018: PloS One
Daria Stypinski, Stephen A McQuarrie, Alexander J B McEwan, Leonard I Wiebe
The objective of this work is to evaluate the potential effect of cardiac stress exercise on the accumulation of [123 I]IAZA, a radiopharmaceutical used to image focal tissue hypoxia, in otherwise normal myocardium in healthy volunteers, and to determine the impact of exercise on [123 I]IAZA pharmacokinetics. The underlying goal is to establish a rational basis and a baseline for studies of focal myocardial hypoxia in cardiac patients using [123 I]IAZA. Three healthy male volunteers ran the 'Bruce' treadmill protocol, a clinically-accepted protocol designed to expose myocardial ischemia in patients...
February 22, 2018: Pharmaceutics
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