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Hplc pharmacokinetics

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https://www.readbyqxmd.com/read/28516478/determination-of-oral-bioavailability-of-curcuminoid-dispersions-and-nanoemulsions-prepared-from-curcuma-longa-linnaeus
#1
P S Lu, B S Inbaraj, B H Chen
BACKGROUND: Curcuminoid from Curcuma longa Linnaeus has been demonstrated to be effective in anti-cancer and anti-inflammation. The objectives of this study were to prepare curcuminoid dispersion and nanoemulsion from C. longa and determine their oral bioavailability in rats. RESULTS: After curcuminoid extraction using 99.5% ethanol, bisdemethoxycurcumin (BDMC), demethoxycurcumin (DMC) and curcumin were separated within 10 min by HPLC with an Eclipse XDB-C18 column and a gradient mobile phase of 0...
May 18, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28513426/pharmacokinetics-and-bioequivalence-of-a-pregabalin-150-mg-capsule-in-healthy-thai-subjects%C3%A2
#2
Nantaporn Prompila, Wanna Eiamart, Yaowatree Jumroen, Nonlanee Sayankuldilok, Pajaree Chariyavilaskul, Wannarasami Ketchat, Supeecha Wittayalertpanya
OBJECTIVE: The purpose of the study was to evaluate the pharmacokinetics and bioequivalence of pregabalin following administration of a 150-mg capsule of test and reference products. METHOD: The study was designed as a randomized, two-treatment, two-period, two-sequence, single-dose crossover with 1-week washout period between period I and period II dosing. 20 healthy male and female Thai subjects were enrolled in the study. Each subject was in fasted state for ~ 10 hours prior to receiving a single oral 150-mg pregabalin capsule...
May 17, 2017: International Journal of Clinical Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28513132/%C3%AE-amylase-and-redox-responsive-nanoparticles-for-tumor-targeted-drug-delivery
#3
Yihui Li, Hang Hu, Qing Zhou, Yanxiao Ao, Chen Xiao, Jiangling Wan, Ying Wan, Huibi Xu, Zifu Li, Xiangliang Yang
Paclitaxel (PTX) is an effective antineoplastic agent and shows potent anti-tumor activity against wide spectrum of cancers. Yet, the wide clinical use of paclitaxel (PTX) is limited by its poor aqueous solubility and side effects associated with current therapeutic formulation. To tackle these obstacles, we, for the first time, report an α-amylase- and redox- responsive nanoparticles based on hydroxyethyl starch (HES) for tumor targeted delivery of PTX. PTX is conjugated onto HES by redox-sensitive disulfide bond to form HES-SS-PTX, which is confirmed with NMR, HPLC-MS, and FT-IR...
May 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28511118/simultaneous-determination-of-selective-serotonin-reuptake-inhibitors-and-their-main-metabolites-in-human-breast-milk-by-liquid-chromatography-electrospray-mass-spectrometry
#4
Etienne Weisskopf, Alice Panchaud, Kim An Nguyen, Delphine Grosjean, Jean-Michel Hascoët, Chantal Csajka, Chin B Eap, Nicolas Ansermot
A bioanalytical method by high performance liquid chromatography coupled to electrospray mass spectrometry (HPLC-ESI-MS), adapted from a previously published method in plasma, was validated in breast milk for the simultaneous quantification of all antidepressants belonging to the class of selective serotonin reuptake inhibitors (citalopram, fluoxetine, fluvoxamine, paroxetine and sertraline) and their major metabolites (desmethylcitalopram and norfluoxetine). Milk samples (250μl) first underwent protein precipitation followed by solid-phase extraction on a reversed phase/cation exchange sorbent...
July 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28505168/development-and-validation-of-reversed-phase-hplc-gradient-method-for-the-estimation-of-efavirenz-in-plasma
#5
Shweta Gupta, Rajesh Kesarla, Narendra Chotai, Abdelwahab Omri
Efavirenz is an anti-viral agent of non-nucleoside reverse transcriptase inhibitor category used as a part of highly active retroviral therapy for the treatment of infections of human immune deficiency virus type-1. A simple, sensitive and rapid reversed-phase high performance liquid chromatographic gradient method was developed and validated for the determination of efavirenz in plasma. The method was developed with high performance liquid chromatography using Waters X-Terra Shield, RP18 50 x 4.6 mm, 3.5 μm column and a mobile phase consisting of phosphate buffer pH 3...
2017: PloS One
https://www.readbyqxmd.com/read/28501167/polyoxometalate-incorporated-polymer-monolith-microextraction-for-highly-selective-extraction-of-antidepressants-in-undiluted-urine
#6
Jiao Cai, Gang-Tian Zhu, Xiao-Mei He, Zheng Zhang, Ren-Qi Wang, Yu-Qi Feng
In this work, a polyoxometalate (POM) incorporated polymer monolith microextraction (PMME) was successfully proposed and employed in the selective extraction of basic antidepressants in undiluted urine sample. This hybrid monolith exhibited strong cation-exchange interaction (SCX) with positively charged antidepressants when pH was 3.0, because of the multiple ionizable moieties on polyanionic POM. As such, antidepressants in complex sample matrices were efficiently extracted by the monolith, and the matrix effect was significantly reduced...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28497121/implementation-of-isavuconazole-in-a-fluorescence-based-high-performance-liquid-chromatography-kit-allowing-simultaneous-detection-of-all-four-currently-licensed-mold-active-triazoles
#7
René Jørgensen, Siri Rytcher Andersen, Karen Marie Thyssen Astvad, Maiken Cavling Arendrup
Isavuconazole (ISZ) is a newly available broad-spectrum triazole agent recently approved for the treatment of both invasive aspergillosis and mucormycosis. The aim of this study was to develop a simple and reliable method for therapeutic drug monitoring (TDM) of ISZ in human plasma samples. The method involves using a kit from ChromSystems intended for TDM of itraconazole (ITZ), posaconazole (PSZ), and voriconazole (VRZ) in serum/plasma for sample preparation and high-performance liquid chromatography, using fluorescence detection with emission and excitation wavelengths set to 261 and 366 nm, respectively...
May 2017: MSphere
https://www.readbyqxmd.com/read/28493402/development-and-validation-of-hplc-ms-ms-method-for-the-determination-of-lixivaptan-in-mouse-plasma-and-its-application-in-a-pharmacokinetic-study
#8
Adnan A Khady, Haithem Alrabiah, Mohamed W Attwa, Sabray Attia, Gamal A E Mostafa
The aim of the present study was to develop a simple, sensitive, and accurate liquid chromatography-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method for the determination of lixivaptan (LIX) in mouse plasma by using vildagliptin as the internal standard (IS). A precipitation procedure was used for the extraction of LIX and vildagliptin from mouse plasma. Chromatographic separation of LIX was achieved using a C18 analytical column (50 mm × 2.1 mm, 1.8 µm) at 25 °C. The mobile phase comprised of acetonitrile and ammonium formate (10 mM, pH 3...
May 10, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28492692/pharmacokinetics-of-table-and-port-red-wine-anthocyanins-a-crossover-trial-in-healthy-men
#9
I Fernandes, C Marques, A Évora, L Cruz, V de Freitas, C Calhau, A Faria, N Mateus
This study was designed to evaluate the pharmacokinetics of Port and table red wine anthocyanins in healthy men. Volunteers were recruited to drink 250 mL of a table red wine (221 mg of anthocyanins) and 150 mL of young Port red wine (49 mg of anthocyanins). Venous blood was collected from participants at 0, 15, 30, 60 and 120 min after wine ingestion. Urine samples were collected at baseline and at 120 min. Anthocyanins and anthocyanin metabolites in plasma and urine samples were quantified by HPLC-DAD and tentatively identified by LC-MS...
May 11, 2017: Food & Function
https://www.readbyqxmd.com/read/28485775/determination-of-phloroglucinol-by-hplc-ms-ms-and-its-application-to-a-bioequivalence-study-in-healthy-volunteers
#10
X-Q Li, R-T Wang, Q-H Wang, X-L Tang, C-T Lu, H-G Gong, A-D Wen
OBJECTIVE: The aim of this study was to compare the pharmacokinetic characteristics of phloroglucinol between an orally disintegrating tablet and an orally lyophilized tablet of phloroglucinol in healthy volunteers under fasting condition. PATIENTS AND METHODS: A rapid and simple method based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method has been developed and validated for the determination of phloroglucinol in human plasma...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28480762/steady-state-pharmacokinetics-of-tenofovir-disoproxil-fumarate-in-human-immunodeficiency-virus-infected-chinese-patients
#11
Xiaoli Du, Huijuan Kou, Qiang Fu, Yanling Li, Zhu Zhu, Taisheng Li
BACKGROUND: The pharmacokinetic (PK) profile of tenofovir disoproxil fumarate in Chinese adults infected with HIV is unknown. RESEARCH DESIGN AND METHODS: A pilot, prospective, open-label study was performed to investigate the steady-state PK profile of tenofovir disoproxil fumarate in 15 Chinese HIV-infected patients among whom eight patients were treated with 300 mg tenofovir disoproxil fumarate, 300 mg lamivudine and 400/100 mg lopinavir/ritonavir, and seven with 300 mg tenofovir disoproxil fumarate, 300 mg lamivudine and 400 mg nevirapine...
May 8, 2017: Expert Review of Clinical Pharmacology
https://www.readbyqxmd.com/read/28470723/pharmacokinetics-milk-penetration-and-pk-pd-analysis-by-monte-carlo-simulation-of-marbofloxacin-after-intravenous-and-intramuscular-administration-to-lactating-goats
#12
A M Lorenzutti, N J Litterio, M A Himelfarb, M D P Zarazaga, M I San Andrés, J J De Lucas
The main objectives of this study were (i) to evaluate the serum pharmacokinetic behaviour and milk penetration of marbofloxacin (MFX; 5 mg/kg), after intravenous (IV) and intramuscular (IM) administration in lactating goats and simulate a multidose regimen on steady-state conditions, (ii) to determine the minimum inhibitory concentration (MIC) and mutant prevention concentration (MPC) of coagulase negative staphylococci (CNS) isolated from caprine mastitis in Córdoba, Argentina and (iii) to make a PK/PD analysis by Monte Carlo simulation from steady-state pharmacokinetic parameters of MFX by IV and IM routes to evaluate the efficacy and risk of the emergence of resistance...
May 4, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28463780/a-novel-liquid-chromatography-tandem-mass-spectrometry-lc-ms-ms-based-bioanalytical-method-for-quantification-of-ethyl-esters-of-eicosapentaenoic-acid-epa-and-docosahexaenoic-acid-dha-and-its-application-in-pharmacokinetic-study
#13
Sekarbabu Viswanathan, P R P Verma, Muniyandithevar Ganesan, Jeganathan Manivannan
Omega-3 fatty acids are clinically useful and the two marine omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are prevalent in fish and fish oils. Omega-3 fatty acid formulations should undergo a rigorous regulatory step in order to obtain United States Food and Drug Administration (USFDA) approval as prescription drug. In connection with that, despite quantifying EPA and DHA fatty acids, there is a need for quantifying the level of ethyl esters of them in biological samples. In this study, we make use of reverse phase high performance liquid chromatography coupled with mass spectrometry (RP-HPLC-MS)technique for the method development...
April 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28460228/a-disposition-kinetic-study-of-tramadol-in-bile-duct-ligated-rats-in-perfused-rat-liver-model
#14
Zohre Esmaeili, Saeid Mohammadi, Alireza Nezami, Mohammad Reza Rouini, Yalda Hosseinzadeh Ardakani, Hoda Lavasani, Mahmoud Ghazi-Khansari
Tramadol hydrochloride is a centrally acting synthetic opioid analgesic drug and is used to treat chronic pain. In this study, the effects of Bile Duct Ligation (BDL) on the pharmacokinetics of tramadol in a liver recirculating perfusion system of male rats were used. Twenty-four Wistar male rats were randomly divided into four groups: control, sham and two weeks BDL and four weeks BDL. Serum levels of liver enzymes were measured before perfusion and the pharmacokinetics of tramadol was evaluated by using liver recirculating perfusion system...
April 28, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28459136/pharmacokinetics-of-grapiprant-a-selective-ep4-prostaglandin-pge2-receptor-antagonist-after-2-mg-kg-oral-and-i-v-administrations-in-cats
#15
B Lebkowska-Wieruszewska, V De Vito, H Owen, A Poapholatep, M Giorgi
Drugs that provide effective analgesia in cats are limited. The aim of the study was to assess the pharmacokinetics of grapiprant after 2 mg/kg administration via p.o. and i.v. routes in cats. Six healthy adult cats were used according to an open, single-dose, two-treatment, two-period, randomized cross-over design. Cats were assigned to two treatment groups and administered with 2 mg/kg of grapiprant (pure powder) through p.o. and i.v. administration. Blood samples were collected at preassigned times and analysed by a validated HPLC method...
April 29, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28457572/analytical-interference-during-cefepime-therapeutic-drug-monitoring-in-intensive-care-patient-about-a-case-report
#16
Laurie Toullec, Julien Dupouey, Coralie Vigne, Amélie Marsot, Laurent Allanioux, Olivier Blin, Marc Leone, Romain Guilhaumou
β-lactams therapeutic drug monitoring (TDM) appears as an essential tool to ensure the achievement of pharmacokinetic-pharmacodynamic targets and prevent induced toxicity in intensive care unit patients. Indeed, those patients exhibit important pharmacokinetic variabilities that could lead to unpredictable plasma concentrations, potentially associated with poor clinical outcome, development of antibiotic resistance or increased side effects. Here, we report the case of a 48-year-old-patient admitted to intensive care unit and treated by cefepime using TDM...
March 30, 2017: Thérapie
https://www.readbyqxmd.com/read/28457009/comparison-of-direct-sampling-and-brochoalveolar-lavage-for-determining-active-drug-concentrations-in-the-pulmonary-epithelial-lining-fluid-of-calves-injected-with-enrofloxacin-or-tilmicosin
#17
D M Foster, H J Sylvester, M G Papich
Antibiotic distribution to interstitial fluid (ISF) and pulmonary epithelial fluid (PELF) was measured and compared to plasma drug concentrations in eight healthy calves. Enrofloxacin (Baytril(®) 100) was administered at a dose of 12.5 mg/kg subcutaneously (SC), and tilmicosin (Micotil(®) 300) was administered at a dose of 20 mg/kg SC. PELF, sampled by two different methods-bronchoalveolar lavage (BAL) and direct sampling (DS)-plasma, and ISF were collected from each calf and measured for tilmicosin, enrofloxacin and its metabolite ciprofloxacin by HPLC...
April 29, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28445409/quantification-of-a-novel-photosensitizer-of-chlorin-e6-c15-monomethyl-ester-in-beagle-dog-plasma-using-hplc-application-to-pharmacokinetic-studies
#18
Xiuxiu Li, Jun Wen, Jingjing Jiang, Xin Zhao, Tingting Zhou, Guorong Fan
Chlorin e6-C15-monomethyl ester (CMME) is a novel photosensitizer, which is synthetized from the degradation products of silkworm excrement. Preclinical studies on the promising photosensitizer CMME are necessary to determine its therapeutic efficacy and druglikeness. A high-performance liquid chromatography with UV detection (HPLC-UV) method was established for the determination of CMME in beagle dog plasma. The sample preparation involved a protein-precipitation method with acetonitrile after the addition of tanshinone IIA as an internal standard (IS)...
April 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28440419/different-effects-of-%C3%A2-borneol-and-%C3%A2-%C3%A2-borneol-on-the-pharmacokinetics-of-osthole-in-rats-following-oral-administration
#19
Dan-Dan Luo, Xiao-Ying Chen, Zhen-Biao Zhang, Chao-Yue Sun, Yi-Feng Zheng, Yu-Hong Liu, Xiu-Fen Wang, Qi Wang, Janis Ya-Xian Zhan, Zi-Ren Su
Osthole is the primary active component of a number of herbal plants such as the Cnidium monnieri fruit. In traditional Chinese herb medicine, osthole is commonly used in combination with borneol to obtain improved pharmacological effects. The aim of the present study was to investigate the effect of borneol enantiomers on the pharmacokinetics of osthole. An appropriate high‑performance liquid chromatography (HPLC) method was applied to determine the concentrations of osthole in plasma. Following oral administration of osthole alone or combined with borneol in rats, blood samples were collected and analyzed by HPLC...
June 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28432941/validation-of-a-spe-hplc-uv-method-for-the-quantification-of-a-new-er-specific-photosensitizer-or-141-in-blood-serum-using-total-error-concept
#20
Emilie Bony, Yohan Mace, Adán Pinto, David Delvaux, Robert Kiss, Olivier Riant, Olivier Feron, Joëlle Quetin-Leclercq
The aim of this work is to develop the first validated HPLC-UV method quantification in blood serum for a new endoplasmic reticulum (ER)-specific benzophenazine photosensitizer (OR-141). A fast solid phase extraction (SPE) cleaning sample procedure was achieved on C18 encapped (ec) SPE cartridges and the separation was performed on a RP-18e column (5μM) using an isocratic elution with methanol. The method has been fully validated according to accuracy profiles based on total error and tolerance intervals. Calibration was performed in the matrix and trueness (<4...
April 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
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