keyword
MENU ▼
Read by QxMD icon Read
search

super-resolution

keyword
https://www.readbyqxmd.com/read/29221906/presynaptic-active-zones-of-mammalian-neuromuscular-junctions-nanoarchitecture-and-selective-impairments-in-aging
#1
REVIEW
Yomna Badawi, Hiroshi Nishimune
Neurotransmitter release occurs at active zones, which are specialized regions of the presynaptic membrane. A dense collection of proteins at the active zone provides a platform for molecular interactions that promote recruitment, docking, and priming of synaptic vesicles. At mammalian neuromuscular junctions (NMJs), muscle-derived laminin β2 interacts with presynaptic voltage-gated calcium channels to organize active zones. The molecular architecture of presynaptic active zones has been revealed using super-resolution microscopy techniques that combine nanoscale resolution and multiple molecular identification...
December 5, 2017: Neuroscience Research
https://www.readbyqxmd.com/read/29221073/beyond-born-rytov-limit-for-super-resolution-optical-diffraction-tomography
#2
JooWon Lim, Abdul Wahab, GwangSik Park, KyeoReh Lee, YongKeun Park, Jong Chul Ye
Optical diffraction tomography (ODT) using Born or Rytov approximation suffers from severe distortions in reconstructed refractive index (RI) tomograms when multiple scattering occurs or the scattering signals are strong. These effects are usually seen as a significant impediment to the application of ODT because multiple scattering is directly linked to an unknown object itself rather than a surrounding medium, and a strong scatter invalidates the underlying assumptions of the Born and Rytov approximations...
November 27, 2017: Optics Express
https://www.readbyqxmd.com/read/29220313/how-does-the-low-rank-matrix-decomposition-help-internal-and-external-learnings-for-super-resolution
#3
Shuang Wang, Bo Yue, Xuefeng Liang, Licheng Jiao
Wisely utilizing the internal and external learning methods is a new challenge in super-resolution problem. To address this issue, we analyze the attributes of two methodologies and find two observations of their recovered details: 1) they are complementary in both feature space and image plane and 2) they distribute sparsely in the spatial space. These inspire us to propose a low-rank solution which effectively integrates two learning methods and then achieves a superior result. To fit this solution, the internal learning method and the external learning method are tailored to produce multiple preliminary results...
March 2018: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/29218789/precise-localizations-of-voltage-gated-sodium-and-potassium-channels-in-neurons
#4
REVIEW
Hiroaki Misonou
Neurons are extremely large and complex cells, and they regulate membrane potentials in multiple subcellular compartments using a variety of ion channels. Voltage-gated sodium (Nav) and potassium (Kv) channels are crucial in regulating neuronal membrane excitability owing to their diversity in subtypes, biophysical properties, and localizations. In particular, specific localizations of Nav and Kv channels in specific membrane compartments are essential to achieve a precise control of local membrane excitability...
December 8, 2017: Developmental Neurobiology
https://www.readbyqxmd.com/read/29216215/accelerating-cross-validation-with-total-variation-and-its-application-to-super-resolution-imaging
#5
Tomoyuki Obuchi, Shiro Ikeda, Kazunori Akiyama, Yoshiyuki Kabashima
We develop an approximation formula for the cross-validation error (CVE) of a sparse linear regression penalized by ℓ1-norm and total variation terms, which is based on a perturbative expansion utilizing the largeness of both the data dimensionality and the model. The developed formula allows us to reduce the necessary computational cost of the CVE evaluation significantly. The practicality of the formula is tested through application to simulated black-hole image reconstruction on the event-horizon scale with super resolution...
2017: PloS One
https://www.readbyqxmd.com/read/29216123/variable-temperature-variable-field-magnetic-circular-photoluminescence-vtvh-mcpl-spectroscopy-for-electronic-structure-determination-in-nanoscale-chemical-systems
#6
Patrick J Herbert, Utsab Mitra, Kenneth L Knappenberger
In this Letter, we describe variable-temperature variable-field magnetic circular photoluminescence (VTVH-MCPL) spectroscopy as a complementary technique to absorption-based magnetic circular dichroism. A paramagnetic model system, Au25(SC8H9)18, is chosen to demonstrate the information content that is obtained from VTVH-MCPL. Specifically, the methods and analyses for the determination of electronic Landé g-factors, zero-field energy splittings, and relative A-, B-, and C-term contributions to the MCPL response are detailed...
December 1, 2017: Optics Letters
https://www.readbyqxmd.com/read/29214255/a-green-light-emitting-spontaneously-blinking-fluorophore-based-on-intramolecular-spirocyclization-for-dual-colour-super-resolution-imaging
#7
Shin-Nosuke Uno, Mako Kamiya, Akihiko Morozumi, Yasuteru Urano
We have developed the first green-light-emitting, spontaneously blinking fluorophore (SBF), HEtetTFER. In combination with our near-infrared-light-emitting SBF (HMSiR), HEtetTFER allows dual-colour single-molecule localization microscopy (SMLM) in buffer solution without any additive and without photoactivation.
December 7, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29212956/icam1-neutrophils-promote-chronic-inflammation-via-asprv1-in-b-cell-dependent-autoimmune-encephalomyelitis
#8
Ryder F Whittaker Hawkins, Alexandre Patenaude, Aline Dumas, Rajiv Jain, Yodit Tesfagiorgis, Steven Kerfoot, Takeshi Matsui, Matthias Gunzer, Patrice E Poubelle, Catherine Larochelle, Martin Pelletier, Luc Vallières
Neutrophils contribute to demyelinating autoimmune diseases, yet their phenotype and functions have been elusive to date. Here, we demonstrate that ICAM1 surface expression distinguishes extra- from intravascular neutrophils in the mouse CNS during experimental autoimmune encephalomyelitis (EAE). Transcriptomic analysis of these 2 subpopulations indicated that neutrophils, once extravasated, acquire macrophage-like properties, including the potential for immunostimulation and MHC class II-mediated antigen presentation...
December 7, 2017: JCI Insight
https://www.readbyqxmd.com/read/29212877/high-speed-super-resolution-imaging-of-the-proteins-in-fission-yeast-clathrin-mediated-endocytic-actin-patches
#9
Rajesh Arasada, Wasim A Sayyad, Julien Berro, Thomas D Pollard
To internalize nutrients and cell surface receptors via clathrin mediated endocytosis cells assemble at least 50 proteins including clathrin, clathrin-interacting proteins, actin filaments and actin binding proteins in a highly ordered and regulated fashion. The molecular mechanism by which actin filament polymerization deforms the cell membrane is unknown, largely due to lack of knowledge about the organization of the regulatory proteins and actin filaments. We used high-speed super-resolution localization microscopy (FPALM) of live fission yeast cells to improve the spatial resolution to ∼35 nm with 1 s temporal resolution...
December 6, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29210582/a-selective-suppression-of-stimulated-raman-scattering-with-another-competing-stimulated-raman-scattering
#10
Doyeon Kim, Dae Sik Choi, Jiwoong Kwon, Sang-Hee Shim, Hanju Rhee, Minhaeng Cho
A three-beam femtosecond stimulated Raman scattering (SRS) scheme is formulated and demonstrated to simultaneously induce two different SRS processes associated with Raman-active modes in the same molecule. Two SR gains involving a common pump pulse are coupled and compete: as one of the Stokes beam intensities increases, the other SRS is selectively suppressed. We provide theoretical description and experimental evidence that the selective suppression behavior is due to the limited number of pump photons used for both two SRS processes when an intense depletion beam induces one SRS process...
December 6, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29203865/albumin-vaccine-nanocomplexes-that-assemble-in-vivo-for-combination-cancer-immunotherapy
#11
Guizhi Zhu, Geoffrey M Lynn, Orit Jacobson, Kai Chen, Yi Liu, Huimin Zhang, Ying Ma, Fuwu Zhang, Rui Tian, Qianqian Ni, Siyuan Cheng, Zhantong Wang, Nan Lu, Bryant C Yung, Zhe Wang, Lixin Lang, Xiao Fu, Albert Jin, Ido D Weiss, Harshad Vishwasrao, Gang Niu, Hari Shroff, Dennis M Klinman, Robert A Seder, Xiaoyuan Chen
Subunit vaccines have been investigated in over 1000 clinical trials of cancer immunotherapy, but have shown limited efficacy. Nanovaccines may improve efficacy but have rarely been clinically translated. By conjugating molecular vaccines with Evans blue (EB) into albumin-binding vaccines (AlbiVax), here we develop clinically promising albumin/AlbiVax nanocomplexes that self-assemble in vivo from AlbiVax and endogenous albumin for efficient vaccine delivery and potent cancer immunotherapy. PET pharmacoimaging, super-resolution microscopies, and flow cytometry reveal almost 100-fold more efficient co-delivery of CpG and antigens (Ags) to lymph nodes (LNs) by albumin/AlbiVax than benchmark incomplete Freund's adjuvant (IFA)...
December 5, 2017: Nature Communications
https://www.readbyqxmd.com/read/29203404/the-imaging-fcs-diffusion-law-in-the-presence-of-multiple-diffusive-modes
#12
Sapthaswaran Veerapathiran, Thorsten Wohland
The cellular plasma membrane is the barrier over which cells exchange materials and communicate with their surroundings, and thus plays the central role in cellular sensing and metabolism. Therefore, the investigation of plasma membrane organization and dynamics is required for understanding of cellular functions. The plasma membrane is a heterogeneous matrix. The presence of structures such as lipid and protein domains and the cytoskeleton meshwork poses a hindrance to the free diffusion of membrane associated biomolecules...
December 1, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29202021/bimetallic-effect-of-single-nanocatalysts-visualized-by-super-resolution-catalysis-imaging
#13
Guanqun Chen, Ningmu Zou, Bo Chen, Justin B Sambur, Eric Choudhary, Peng Chen
Compared with their monometallic counterparts, bimetallic nanoparticles often show enhanced catalytic activity associated with the bimetallic interface. Direct quantitation of catalytic activity at the bimetallic interface is important for understanding the enhancement mechanism, but challenging experimentally. Here using single-molecule super-resolution catalysis imaging in correlation with electron microscopy, we report the first quantitative visualization of enhanced bimetallic activity within single bimetallic nanoparticles...
November 22, 2017: ACS Central Science
https://www.readbyqxmd.com/read/29196704/repurposing-a-photosynthetic-antenna-protein-as-a-super-resolution-microscopy-label
#14
Samuel F H Barnett, Andrew Hitchcock, Amit K Mandal, Cvetelin Vasilev, Jonathan M Yuen, James Morby, Amanda A Brindley, Dariusz M Niedzwiedzki, Donald A Bryant, Ashley J Cadby, Dewey Holten, C Neil Hunter
Techniques such as Stochastic Optical Reconstruction Microscopy (STORM) and Structured Illumination Microscopy (SIM) have increased the achievable resolution of optical imaging, but few fluorescent proteins are suitable for super-resolution microscopy, particularly in the far-red and near-infrared emission range. Here we demonstrate the applicability of CpcA, a subunit of the photosynthetic antenna complex in cyanobacteria, for STORM and SIM imaging. The periodicity and width of fabricated nanoarrays of CpcA, with a covalently attached phycoerythrobilin (PEB) or phycocyanobilin (PCB) chromophore, matched the lines in reconstructed STORM images...
December 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29196473/cell-scientist-to-watch-lukas-kapitein
#15
(no author information available yet)
Lukas Kapitein studied physics at the VU University in Amsterdam and graduated with a master's degree in experimental physics. He went on to do his PhD in the research groups of Christoph Schmidt and Erwin Peterman on the motility of mitotic kinesins, and in 2007, he won the biannual best thesis award from the Dutch society for Biophysics and Biomedical Technology. Lukas then joined the laboratory of Casper Hoogenraad at the Erasmus Medical Center in Rotterdam to work on the cytoskeleton and polarised cargo transport in neurons...
December 1, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/29193432/spatial-separation-of-ftsz-and-ftsn-during-cell-division
#16
Bill Söderström, Helena Chan, Patrick J Shilling, Ulf Skoglund, Daniel O Daley
The division of Escherichia coli is mediated by a collection of some 34 different proteins that are recruited to the division septum and are thought to assemble into a macromolecular complex known as 'the divisome'. Herein we have endeavored to better understand the structure of the divisome by imaging two of its core components; FtsZ and FtsN. Super resolution microscopy (SIM and gSTED) indicated that both proteins are localized in large assemblies, which are distributed around the division septum (i.e. forming a discontinuous ring)...
November 29, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/29193244/an-efficient-sequence-for-fetal-brain-imaging-at-3t-with-enhanced-t1-contrast-and-motion-robustness
#17
Giulio Ferrazzi, Anthony N Price, Rui Pedro A G Teixeira, Lucilio Cordero-Grande, Jana Hutter, Ana Gomes, Francesco Padormo, Emer Hughes, Torben Schneider, Mary Rutherford, Maria Kuklisova Murgasova, Joseph V Hajnal
PURPOSE: Ultrafast single-shot T2 -weighted images are common practice in fetal MR exams. However, there is limited experience with fetal T1 -weighted acquisitions. This study aims at establishing a robust framework that allows fetal T1 -weighted scans to be routinely acquired in utero at 3T. METHODS: A 2D gradient echo sequence with an adiabatic inversion was optimized to be robust to fetal motion and maternal breathing optimizing grey/white matter contrast at the same time...
November 28, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29192161/unusual-scaling-laws-for-plasmonic-nanolasers-beyond-the-diffraction-limit
#18
Suo Wang, Xing-Yuan Wang, Bo Li, Hua-Zhou Chen, Yi-Lun Wang, Lun Dai, Rupert F Oulton, Ren-Min Ma
Plasmonic nanolasers are a new class of amplifiers that generate coherent light well below the diffraction barrier bringing fundamentally new capabilities to biochemical sensing, super-resolution imaging, and on-chip optical communication. However, a debate about whether metals can enhance the performance of lasers has persisted due to the unavoidable fact that metallic absorption intrinsically scales with field confinement. Here, we report plasmonic nanolasers with extremely low thresholds on the order of 10 kW cm-2 at room temperature, which are comparable to those found in modern laser diodes...
December 1, 2017: Nature Communications
https://www.readbyqxmd.com/read/29190677/cortical-actin-nodes-their-dynamics-and-recruitment-of-podosomal-proteins-as-revealed-by-super-resolution-and-single-molecule-microscopy
#19
Yuki M Shirai, Taka A Tsunoyama, Nao Hiramoto-Yamaki, Koichiro M Hirosawa, Akihiro C E Shibata, Kenichi Kondo, Atsushi Tsurumune, Fumiyoshi Ishidate, Akihiro Kusumi, Takahiro K Fujiwara
Electron tomography of the plasma membrane (PM) identified several layers of cortical actin meshwork running parallel to the PM cytoplasmic surface throughout the PM. Here, cortical actin structures and dynamics were examined in living cells, using super-resolution microscopy, with (x,y)- and z-resolutions of ~140 and ~400 nm, respectively, and single-molecule imaging. The super-resolution microscopy identified sub-micron-sized actin clusters that appeared identical by both phalloidin post-fixation staining and Lifeact-mGFP expression followed by fixation, and therefore, these actin clusters were named "actin-pl-clusters"...
2017: PloS One
https://www.readbyqxmd.com/read/29189775/visualizing-endocytic-recycling-and-trafficking-in-live-neurons-by-subdiffractional-tracking-of-internalized-molecules
#20
Merja Joensuu, Ramon Martínez-Mármol, Pranesh Padmanabhan, Nick R Glass, Nela Durisic, Matthew Pelekanos, Mahdie Mollazade, Giuseppe Balistreri, Rumelo Amor, Justin J Cooper-White, Geoffrey J Goodhill, Frédéric A Meunier
Our understanding of endocytic pathway dynamics is restricted by the diffraction limit of light microscopy. Although super-resolution techniques can overcome this issue, highly crowded cellular environments, such as nerve terminals, can also dramatically limit the tracking of multiple endocytic vesicles such as synaptic vesicles (SVs), which in turn restricts the analytical dissection of their discrete diffusional and transport states. We recently introduced a pulse-chase technique for subdiffractional tracking of internalized molecules (sdTIM) that allows the visualization of fluorescently tagged molecules trapped in individual signaling endosomes and SVs in presynapses or axons with 30- to 50-nm localization precision...
December 2017: Nature Protocols
keyword
keyword
67790
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"