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https://www.readbyqxmd.com/read/28222005/a-modular-low-complexity-ecg-delineation-algorithm-for-real-time-embedded-systems
#1
Jose Manuel Bote, Joaquin Recas, Francisco Rincon, David Atienza, Roman Hermida
This work presents a new modular and lowcomplexity algorithm for the delineation of the different ECG waves (QRS, P and T peaks, onsets and end). Involving a reduced number of operations per second and having a small memory footprint, this algorithm is intended to perform realtime delineation on resource-constrained embedded systems. The modular design allows the algorithm to automatically adjust the delineation quality in run time to a wide range of modes and sampling rates, from a Ultra-low power mode when no arrhythmia is detected, in which the ECG is sampled at low frequency, to a complete High-accuracy delineation mode in which the ECG is sampled at high frequency and all the ECG fiducial points are detected, in case of arrhythmia...
February 17, 2017: IEEE Journal of Biomedical and Health Informatics
https://www.readbyqxmd.com/read/28220509/a-novel-symbiovar-aegeanense-of-the-genus-ensifer-nodulates-vigna-unguiculata
#2
Anastasia P Tampakaki, Christos T Fotiadis, Georgia Ntatsi, Dimitrios Savvas
BACKGROUND: Cowpea (Vigna unguiculata) forms nitrogen-fixing root nodules with diverse symbiotic bacteria, mainly slow-growing rhizobial species belonging to the genus Bradyrhizobium, although few studies have reported the isolation of fast-growing rhizobia under laboratory and field conditions. Despite that much research has been done on cowpea-nodulating bacteria in various countries around the world, very limited information is available on cowpea rhizobia in European soils. The aim of this study was to study the genetic and phenotypic diversity of indigenous cowpea-nodulating rhizobia in Greece...
February 21, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28214058/phylogenetic-multilocus-sequence-analysis-of-indigenous-slow-growing-rhizobia-nodulating-cowpea-vigna-unguiculata-l-in-greece
#3
Anastasia P Tampakaki, Christos T Fotiadis, Georgia Ntatsi, Dimitrios Savvas
Cowpea (Vigna unguiculata) is a promiscuous grain legume, capable of establishing efficient symbiosis with diverse symbiotic bacteria, mainly slow-growing rhizobial species belonging to the genus Bradyrhizobium. Although much research has been done on cowpea-nodulating bacteria in various countries around the world, little is known about the genetic and symbiotic diversity of indigenous cowpea rhizobia in European soils. In the present study, the genetic and symbiotic diversity of indigenous rhizobia isolated from field-grown cowpea nodules in three geographically different Greek regions were studied...
February 2, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28209394/ensifer-shofinae-sp-nov-a-novel-rhizobial-species-isolated-from-root-nodules-of-soybean-glycine-max
#4
Wen Hao Chen, Sheng Hui Yang, Zhao Hu Li, Xiao Xia Zhang, Xin Hua Sui, En Tao Wang, Wen Xin Chen, Wen Feng Chen
Two bacterial strains isolated from root nodules of soybean were characterized phylogenetically as members of a distinct group in the genus Ensifer based on 16S rRNA gene comparisons. They were also verified as a separated group by the concatenated sequence analyses of recA, atpD and glnII (with similarities ≤93.9% to the type strains for defined species), and by the average nucleotide identities (ANI) between the whole genome sequence of the representative strain CCBAU 251167(T) and those of the closely related strains in Ensifer glycinis and Ensifer fredii (90...
February 2, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28204854/adsorption-of-dna-binding-proteins-to-functionalized-carbon-nanotube-surfaces-with-and-without-dna-wrapping
#5
Yu Ishibashi, Shusuke Oura, Kazuo Umemura
We examined the adsorption of DNA binding proteins on functionalized, single-walled carbon nanotubes (SWNTs). When SWNTs were functionalized with polyethylene glycol (PEG-SWNT), moderate adsorption of protein molecules was observed. In contrast, nanotubes functionalized with CONH2 groups (CONH2-SWNT) exhibited very strong interactions between the CONH2-SWNT and DNA binding proteins. Instead, when these SWNT surfaces were wrapped with DNA molecules (thymine 30-mers), protein binding was a little decreased. Our results revealed that DNA wrapped PEG-SWNT was one of the most promising candidates to realize DNA nanodevices involving protein reactions on DNA-SWNT surfaces...
February 15, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28180951/bradyrhizobium-centrolobii-and-bradyrhizobium-macuxiense-sp-nov-isolated-from-centrolobium-paraense-grown-in-soil-of-amazonia-brazil
#6
Daniele C Michel, Samuel R Passos, Jean L Simões-Araujo, Alexandre C Baraúna, Krisle da Silva, Marcia M Parma, Itamar S Melo, Sofie E De Meyer, Graham O'Hara, Jerri E Zilli
Thirteen Gram-negative, aerobic, motile with polar flagella, rod-shaped bacteria were isolated from root nodules of Centrolobium paraense Tul. grown in soils from the Amazon region of Brazil. Growth of strains was observed at temperature range 20-36 °C (optimal 28 °C), pH ranges 5-11 (optimal 6.0-7.0), and 0.1-0.5%NaCl (optimal 0.1-0.3%). Analysis of 16S rRNA gene placed the strains into two groups within Bradyrhizobium. Closest neighbouring species (98.8%) for group I was B. neotropicale while for group II were 12 species with more than 99% of similarity...
February 8, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28163823/draft-genome-sequence-of-type-strain-hbr26-t-and-description-of-rhizobium-aethiopicum-sp-nov
#7
Aregu Amsalu Aserse, Tanja Woyke, Nikos C Kyrpides, William B Whitman, Kristina Lindström
Rhizobium aethiopicum sp. nov. is a newly proposed species within the genus Rhizobium. This species includes six rhizobial strains; which were isolated from root nodules of the legume plant Phaseolus vulgaris growing in soils of Ethiopia. The species fixes nitrogen effectively in symbiosis with the host plant P. vulgaris, and is composed of aerobic, Gram-negative staining, rod-shaped bacteria. The genome of type strain HBR26(T) of R. aethiopicum sp. nov. was one of the rhizobial genomes sequenced as a part of the DOE JGI 2014 Genomic Encyclopedia project designed for soil and plant-associated and newly described type strains...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28154946/rhizobium-rhizosphaerae-sp-nov-a-novel-species-isolated-from-rice-rhizosphere
#8
Juan-Juan Zhao, Jun Zhang, Rui-Jie Zhang, Cai-Wen Zhang, Hua-Qun Yin, Xiao-Xia Zhang
Two novel, Gram-negative, motile, rod-shaped, aerobic bacterial strains, MH17(T) and RD15, were isolated from the sterilized root and rhizosphere soil of rice, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that the similarity between strains MH17(T) and RD15 was 100%. The isolates exhibit high sequence similarities to Rhizobium oryzae CGMCC 1.7048(T) (98.7%) and Rhizobium petrolearium SL-1(T) (97.0% and 97.1%), which supports that they belong to a novel species in the genus Rhizobium...
February 2, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28150571/sanguibacter-gelidistatuariae-sp-nov-a-novel-psychrotolerant-anaerobe-from-an-ice-sculpture-in-antarctica-and-emendation-of-descriptions-for-the-family-sanguibacteraceae-the-genus-sanguibacter-and-species-s-antarcticus-s-inulinus-s-kedieii-s-marinus-s-soli
#9
Elena V Pikuta, Zhe Lyu, Melissa D Williams, Nisha B Patel, Yuchen Liu, Richard Brice Hoover, Hans-Jürgen Busse, Paul A Lawson, William B Whitman
A novel psychrotolerant bacterium strain ISLP-3T was isolated from a sample of naturally formed ice sculpture on the shore of Lake Podprudnoye in Antarctica. Cells were motile, stained Gram positive, non-spore-forming, straight or slightly curved rods with the shape of a baseball bat. The new isolate was facultatively anaerobic and catalase positive. Growth occurred at 3-35 ºC with an optimum at 24 °C, 0-2 % w/v NaCl with an optimum at 0.3 % and pH 6.2 - 9.5 with an optimum 7.5. Strain ISLP-3T grew on several carbon sources, with the best growth on D-cellobiose...
February 1, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28138100/transposase-mediated-excision-conjugative-transfer-and-diversity-of-ice6013-in-staphylococcus-aureus
#10
Emily A Sansevere, Xiao Luo, Joo Youn Park, Sunghyun Yoon, Keun Seok Seo, D Ashley Robinson
: ICE6013 represents one of two families of integrative conjugative elements (ICEs) identified in the pan-genome of the human and animal pathogen, Staphylococcus aureus Here we investigate the excision and conjugation functions of ICE6013 and further characterize the diversity of this element. ICE6013 excision is not significantly affected by growth, temperature, pH or UV exposure and does not depend on recA The IS30-like DDE transposase (Tpase; orf1 and orf2) of ICE6013 must be uninterrupted for excision to occur, whereas disruption of three other orfs on the element significantly affect the level of excision...
January 30, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28126039/rhizobium-arenae-sp-nov-isolated-from-the-sand-of-desert-mu-us-china
#11
Shengnan Zhang, Shanshan Yang, Wei Chen, Yong Chen, Mingjuan Zhang, Xinai Zhou, Guohua Fan, Fu Ying Feng
A Gram-strain-negative, rod-shaped, motile bacterium, designated MIM27T, was isolated from the sand of Desert Mu Us, China. The strain could grow at 4-45 °C (optimum, 37 °C), at pH 6.5-9.0 (optimum, 8.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %). Phylogenetic analysis on 16S rRNA gene sequence demonstrated that the strain was belonged to the genus Rhizobium, with the highest similarity (96.5 %) to Rhizobium pakistanensis BN-19T. Analysis of nitrogen fixation gene nifH and three housekeeping gene sequences of recA, atpD and glnII were also showed that strain MIM27T was mostly related to the recognized species of the genus Rhizobium but the similarities were low ( 90...
January 23, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28120200/kribbella-soli-sp-nov-isolated-from-soil
#12
Fadime Ozdemir-Kocak, Hayrettin Saygin, Salih Saricaoglu, Demet Cetin, Kiymet Guven, Cathrin Spröer, Peter Schumann, Hans-Peter Klenk, Nevzat Sahin, Kamil Isik
A novel actinobacterial strain, designated FMN22(T), was isolated from soil and characterised using a polyphasic approach. Strain FMN22(T) showed high 16S rRNA gene sequence similarity to Kribbella karoonensis Q41(T) (99.3%), Kribbella shirazensis UTMC 693(T) (99.0%), Kribbella aluminosa HKI 0478(T) (98.9%) and Kribbella hippodromi S1.4(T) (98.6%). Phylogenetic analysis using the 16S rRNA and concatenated gene (gyrB, rpoB, relA, recA and atpD) sequences showed that strain FMN22(T) is closely related to the type strains of K...
January 24, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28120190/oxidative-stress-response-of-deinococcus-geothermalis-via-a-cystine-importer
#13
Minwook Kim, Sunwook Jeong, Sangyong Lim, Jeonggu Sim, Ho-Gun Rhie, Sung-Jae Lee
A cystine-dependent anti-oxidative stress response is characterized in Deinococcus geothermalis for the first time. Nevertheless, the same transcriptional directed Δdgeo_1985F mutant strain was revealed to have an identical phenotype to the wild-type strain, while the reverse transcriptional directed Δdgeo_1985R mutant strain was more resistant to oxidative stress at a certain concentration of H2O2 than the wild-type strain. The wild-type and mutant strains expressed equal levels of superoxide dismutase and catalase under H2O2-induced stress...
February 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28115633/allosteric-regulation-of-helicase-core-activities-of-the-dead-box-helicase-yxin-by-rna-binding-to-its-rna-recognition-motif
#14
Brighton Samatanga, Alexandra Z Andreou, Dagmar Klostermeier
DEAD-box proteins share a structurally similar core of two RecA-like domains (RecA_N and RecA_C) that contain the conserved motifs for ATP-dependent RNA unwinding. In many DEAD-box proteins the helicase core is flanked by ancillary domains. To understand the regulation of the DEAD-box helicase YxiN by its C-terminal RNA recognition motif (RRM), we investigated the effect of RNA binding to the RRM on its position relative to the core, and on core activities. RRM/RNA complex formation substantially shifts the RRM from a position close to the RecA_C to the proximity of RecA_N, independent of RNA contacts with the core...
January 23, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28113049/treponema-ruminis-sp-nov-a-spirochaete-isolated-from-the-bovine-rumen
#15
Kerry Newbrook, Gareth J Staton, Simon R Clegg, Richard J Birtles, Stuart D Carter, Nicholas J Evans
A novel bacterium, Ru1T, was encountered during a survey of spirochaetes living in the gastrointestinal (GI) tract of ruminants. Comparative analysis of 16S rRNA gene sequence data indicated that Ru1T clustered within the Treponema genus but shared at most 86.1% sequence similarity with other recognised Treponema species. Further phylogenetic analysis based on partial recombinase A (recA) gene sequence comparisons, together with phenotypic characterisation, also demonstrated the divergence of Ru1T from other recognised Treponema species...
January 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28112216/enhancement-of-reca-mediated-self-assembly-in-dna-nanostructures-through-basepair-mismatches-and-single-strand-nicks
#16
Sybilla Louise Corbett, Rajan Sharma, Alexander Giles Davies, Christoph Wälti
The use of DNA as a structural material for nanometre-scale construction has grown extensively over the last decades. The development of more advanced DNA-based materials would benefit from a modular approach enabling the direct assembly of additional elements onto nanostructures after fabrication. RecA-based nucleoprotein filaments encapsulating short ssDNA have been demonstrated as a tool for highly efficient and fully programmable post-hoc patterning of duplex DNA scaffold. However, the underlying assembly process is not fully understood, in particular when patterning complex DNA topologies...
January 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28111726/ganetespib-overcomes-resistance-to-parp-inhibitors-in-breast-cancer-by-targeting-core-proteins-in-the-dna-repair-machinery
#17
Juhong Jiang, Yuanzhi Lu, Zhi Li, Liping Li, Daoli Niu, Wenwei Xu, Jing Liu, Lin Fu, Ziqing Zhou, Yingying Gu, Fen Xia
DNA damage repair plays essential roles in drug resistance, especially resistance to Poly (ADP-ribose) polymerase (PARP) inhibitors in the clinic. A subset of DNA repair proteins such as Breast cancer gene 1 (BRCA1), BRCA2 and RecA homolog (RAD51) are client proteins of heat shock protein 90 (Hsp90). Clearance of these DNA repair proteins by inhibition of Hsp90 is a promising strategy for overcoming resistance to PARP inhibitors. Here we report the pharmacological analysis of the highly potent second-generation Hsp90 inhibitor, ganetespib...
January 23, 2017: Investigational New Drugs
https://www.readbyqxmd.com/read/28104332/a-bacillus-sp-strain-with-antagonistic-activity-against-fusarium-graminearum-kills-microcystis-aeruginosa-selectively
#18
Huanling Xuan, Xianzhu Dai, Jing Li, Xiaohui Zhang, Caiyun Yang, Feng Luo
Cyanobacterial harmful algal blooms (CyanoHABs) cause severe environmental problems, economic losses and threaten human health seriously. In the present study, a Bacillus sp. strain, designated as AF-1, with strong antagonistic activity against plant pathogenic fungus Fusarium graminearum was isolated from purple soil. Bacillus sp. AF-1 selectively killed Microcystis aeruginosa at low cell density (1.6×10(3)cfu/mL), and showed the strongest bactericidal activity against M. aeruginosa NIES-843 (Ae=93%, t=6d)...
January 16, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28101376/atpase-activity-tightly-regulates-reca-nucleofilaments-to-promote-homologous-recombination
#19
Bailin Zhao, Dapeng Zhang, Chengmin Li, Zheng Yuan, Fangzhi Yu, Shangwei Zhong, Guibin Jiang, Yun-Gui Yang, X Chris Le, Michael Weinfeld, Ping Zhu, Hailin Wang
Homologous recombination (HR), catalyzed in an evolutionarily conserved manner by active RecA/Rad51 nucleofilaments, maintains genomic integrity and promotes biological evolution and diversity. The structures of RecA/Rad51 nucleofilaments provide information critical for the entire HR process. By exploiting a unique capillary electrophoresis-laser-induced fluorescence polarization assay, we have discovered an active form of RecA nucleofilament, stimulated by ATP hydrolysis, that contains mainly unbound nucleotide sites...
2017: Cell Discovery
https://www.readbyqxmd.com/read/28097539/rhizobium-hedysari-sp-nov-a-novel-species-isolated-from-a-root-nodule-of-hedysarum-multijugum-in-china
#20
Lin Xu, Jianfeng Shi, Caixia Li, Shengan Zhu, Bo Li
A strain 5-1-2(T) was isolated from a root nodule of Hedysarum multijugum collected from Zhangye city, Gansu province, north-west China. Phylogenetic analysis based on the 16S rRNA gene sequence and other housekeeping genes (recA and atpD) indicated that the strain represents a novel species in the genus Rhizobium close to the strain Rhizobium subbaraonis JC85(T) with similarities of 98.27, 88.92 and 89.62%, respectively. Strain 5-1-2(T) contained Q-10 as the predominant ubiquinone. Our results showed that the major fatty acids were feature 8 (C18:1 ω7c and/or C18:1 ω6c; 38...
January 17, 2017: Antonie Van Leeuwenhoek
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