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https://www.readbyqxmd.com/read/28542496/zinc-blocks-sos-induced-antibiotic-resistance-via-inhibition-of-reca-in-escherichia-coli
#1
Bryan E Bunnell, Jillian F Escobar, Kirsten L Bair, Mark D Sutton, John K Crane
Zinc inhibits the virulence of diarrheagenic E. coli by inducing the envelope stress response and inhibiting the SOS response. The SOS response is triggered by damage to bacterial DNA. In Shiga-toxigenic E. coli, the SOS response strongly induces the production of Shiga toxins (Stx) and of the bacteriophages that encode the Stx genes. In E. coli, induction of the SOS response is accompanied by a higher mutation rate, called the mutator response, caused by a shift to error-prone DNA polymerases when DNA damage is too severe to be repaired by canonical DNA polymerases...
2017: PloS One
https://www.readbyqxmd.com/read/28542203/correction-genome-functional-gene-annotation-and-nuclear-transformation-of-the-heterokont-oleaginous-alga-nannochloropsis-oceanica-ccmp1779
#2
Astrid Vieler, Guangxi Wu, Chia-Hong Tsai, Blair Bullard, Adam J Cornish, Christopher Harvey, Ida-Barbara Reca, Chelsea Thornburg, Rujira Achawanantakun, Christopher J Buehl, Michael S Campbell, David Cavalier, Kevin L Childs, Teresa J Clark, Rahul Deshpande, Erika Erickson, Ann Armenia Ferguson, Witawas Handee, Que Kong, Xiaobo Li, Bensheng Liu, Steven Lundback, Cheng Peng, Rebecca L Roston, Sanjaya, Jeffrey P Simpson, Allan TerBush, Jaruswan Warakanont, Simone Zäuner, Eva M Farre, Eric L Hegg, Ning Jiang, Min-Hao Kuo, Yan Lu, Krishna K Niyogi, John Ohlrogge, Katherine W Osteryoung, Yair Shachar-Hill, Barbara B Sears, Yanni Sun, Hideki Takahashi, Mark Yandell, Shin-Han Shiu, Christoph Benning
[This corrects the article DOI: 10.1371/journal.pgen.1003064.].
May 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28536148/the-lotus-domain-is-a-conserved-dead-box-rna-helicase-regulator-essential-for-the-recruitment-of-vasa-to-the-germ-plasm-and-nuage
#3
Mandy Jeske, Christoph W Müller, Anne Ephrussi
DEAD-box RNA helicases play important roles in a wide range of metabolic processes. Regulatory proteins can stimulate or block the activity of DEAD-box helicases. Here, we show that LOTUS (Limkain, Oskar, and Tudor containing proteins 5 and 7) domains present in the germline proteins Oskar, TDRD5 (Tudor domain-containing 5), and TDRD7 bind and stimulate the germline-specific DEAD-box RNA helicase Vasa. Our crystal structure of the LOTUS domain of Oskar in complex with the C-terminal RecA-like domain of Vasa reveals that the LOTUS domain occupies a surface on a DEAD-box helicase not implicated previously in the regulation of the enzyme's activity...
May 23, 2017: Genes & Development
https://www.readbyqxmd.com/read/28532268/allergen-immunotherapy-in-allergic-rhinitis-current-use-and-future-trends
#4
Ludger Klimek, Oliver Pfaar, Jean Bousquet, Gabriela Senti, Thomas Kündig
Type-1 allergies are among the most chronic common diseases of humans. Allergen immunotherapy (AIT) is the only causative and disease-modifying treatment option besides allergen avoidance. Severe systemic adverse allergic reactions may be induced by every AIT treatment. Different approaches have been used to provide safer AIT preparations to lower or even totally overcome this risk. Areas covered: A structured literature recherche in Medline and Pubmed under inclusion of national and international guidelines and Cochrane meta-analyses has been performed aiming at reviewing clinical use of such approaches in AIT...
May 23, 2017: Expert Review of Clinical Immunology
https://www.readbyqxmd.com/read/28527403/interplay-between-bacillus-subtilis-recd2-and-the-recg-or-ruvab-helicase-in-recombinational-repair
#5
Rubén Torres, Hector Romero, Violeta Rodríguez-Cerrato, Juan C Alonso
Bacillus subtilis AddAB, RecS, RecQ, PcrA, HelD, DinG, RecG, RuvAB, PriA and RecD2 are genuine recombinational repair enzymes, but the biological role of RecD2 is poorly defined. A ΔrecD2 mutation sensitizes cells to DNA-damaging agents that stall or collapse replication forks. We found that this ΔrecD2 mutation impaired growth, and that a mutation in the pcrA gene (pcrA596) relieved this phenotype. The ΔrecD2 mutation was not epistatic to ΔaddAB, ΔrecQ, ΔrecS, ΔhelD, pcrA596 and ΔdinG, but epistatic to recA...
May 12, 2017: DNA Repair
https://www.readbyqxmd.com/read/28526895/three-novel-xenorhabdus-steinernema-associations-and-evidence-of-strains-of-x-khoisanae-switching-between-different-clades
#6
Jonike Dreyer, Antoinette P Malan, Leon M T Dicks
Xenorhabdus species are normally closely associated with entomopathogenic nematodes of the family Steinernematidae. Strain F2, isolated from Steinernema nguyeni, was identified as Xenorhabdus bovienii and strains J194 and SB10, isolated from Steinernema jeffreyense and Steinernema sacchari as Xenorhabdus khoisanae, based on phenotypic characteristics and sequencing of 16S rRNA and housekeeping genes dnaN, gltX, gyrB, infB and recA. All three strains produced antimicrobial compounds that inhibited the growth of Gram-positive and Gram-negative bacteria...
May 19, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28515453/validation-of-reference-genes-for-the-normalization-of-the-rt-qpcr-gene-expression-of-virulence-genes-of-erwinia-amylovora-in-apple-shoots
#7
Monika Kałużna, Anita Kuras, Joanna Puławska
To study the expression of pathogenicity-related genes in Erwinia amylovora, seven candidate reference genes (ffh, glyA, gyrA, proC, pykA, recA, rpoB) were selected and validated with the following five different mathematic algorithms: geNorm, NormFinder, BestKeeper, the delta CT method and the RefFinder web-based tool. An overall comprehensive ranking output from each of the selected software programs revealed that proC and recA, followed by ffh and pykA, were the most stably expressed genes and can be recommended for the normalization of RT-qPCR data...
May 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28515022/sos-response-in-bacteria-inhibitory-activity-of-lichen-secondary-metabolites-against-escherichia-coli-reca-protein
#8
Pierangelo Bellio, Letizia Di Pietro, Alisia Mancini, Marisa Piovano, Marcello Nicoletti, Fabrizia Brisdelli, Donatella Tondi, Laura Cendron, Nicola Franceschini, Gianfranco Amicosante, Mariagrazia Perilli, Giuseppe Celenza
BACKGROUND: RecA is a bacterial multifunctional protein essential to genetic recombination, error-prone replicative bypass of DNA damages and regulation of SOS response. The activation of bacterial SOS response is directly related to the development of intrinsic and/or acquired resistance to antimicrobials. Although recent studies directed towards RecA inactivation via ATP binding inhibition described a variety of micromolar affinity ligands, inhibitors of the DNA binding site are still unknown...
June 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28513583/the-cohesin-like-recn-protein-stimulates-reca-mediated-recombinational-repair-of-dna-double-strand-breaks
#9
Lee A Uranga, Emigdio D Reyes, Praveen L Patidar, Lindsay N Redman, Shelley L Lusetti
RecN is a cohesin-like protein involved in DNA double-strand break repair in bacteria. The RecA recombinase functions to mediate repair via homologous DNA strand invasion to form D-loops. Here we provide evidence that the RecN protein stimulates the DNA strand invasion step of RecA-mediated recombinational DNA repair. The intermolecular DNA tethering activity of RecN protein described previously cannot fully explain this novel activity since stimulation of RecA function is species-specific and requires RecN ATP hydrolysis...
May 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/28512381/first-report-and-comparative-study-of-steinernema-surkhetense-rhabditida-steinernematidae-and-its-symbiont-bacteria-from-subcontinental-india
#10
Aashiq Hussain Bhat, Istkhar, Ashok Kumar Chaubey, Vladimir Půža, Ernesto San-Blas
Two populations (CS19 and CS20) of entomopathogenic nematodes were isolated from the soils of vegetable fields from Bijnor district, India. Based on morphological, morphometrical, and molecular studies, the nematodes were identified as Steinernema surkhetense. This work represents the first report of this species in India. The infective juveniles (IJs) showed morphometrical and morphological differences, with the original description based on longer IJs size. The IJs of the Indian isolates possess six ridges in their lateral field instead of eight reported in the original description...
March 2017: Journal of Nematology
https://www.readbyqxmd.com/read/28511132/activity-and-in-vivo-dynamics-of-bacillus-subtilis-disa-are-affected-by-rada-sms-and-by-holliday-junction-processing-proteins
#11
Carolina Gándara, Daniella K C de Lucena, Rubén Torres, Ester Serrano, Stephan Altenburger, Peter L Graumann, Juan C Alonso
Bacillus subtilis c-di-AMP synthase DisA and RecA-related RadA/Sms are involved in the repair of DNA damage in exponentially growing cells. We provide genetic evidence that DisA or RadA/Sms is epistatic to the branch migration translocase (BMT) RecG and the Holliday junction (HJ) resolvase RecU in response to DNA damage. We provide genetic evidence damage. Functional DisA-YFP formed dynamic foci in exponentially growing cells, which moved through the nucleoids at a speed compatible with a DNA-scanning mode...
May 5, 2017: DNA Repair
https://www.readbyqxmd.com/read/28505340/members-of-microvirga-and-bradyrhizobium-genera-are-native-endosymbiotic-bacteria-nodulating-lupinus-luteus-in-northern-tunisian-soils
#12
A Msaddak, M Rejili, D Durán, L Rey, J Imperial, J Palacios, T Ruiz-Argüeso, M Mars
The genetic diversity of bacterial populations nodulating Lupinus luteus (yellow lupine) in Northern Tunisia was examined. Phylogenetic analyses of forty three isolates based on recA and gyrB partial sequences grouped them in three clusters, two of which belong to genus Bradyrhizobium (41 isolates) and one, remarkably, to Microvirga (2 isolates), a genus never previously described as microsymbiont of this lupine species. Representatives of the three clusters were analysed in-depth by multilocus sequence analysis (MLSA) of five housekeeping genes (rrs, recA, glnII, gyrB and dnaK)...
May 15, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28503377/analysis-of-reca-independent-recombination-events-between-short-direct-repeats-related-to-a-genomic-island-and-to-a-plasmid-in-escherichia-coli-k12
#13
María F Azpiroz, Magela Laviña
RecA-independent recombination events between short direct repeats, leading to deletion of the intervening sequences, were found to occur in two genetic models in the Escherichia coli K12 background. The first model was a small E. coli genomic island which had been shown to be mobile in its strain of origin and, when cloned, also in the E. coli K12 context. However, it did not encode a site-specific recombinase as mobile genomic islands usually do. It was then deduced that the host cells should provide the recombination function...
2017: PeerJ
https://www.readbyqxmd.com/read/28501701/radb-acts-in-homologous-recombination-in-the-archaeon-haloferax-volcanii-consistent-with-a-role-as-recombination-mediator
#14
Kayleigh Wardell, Sam Haldenby, Nathan Jones, Susan Liddell, Greg H P Ngo, Thorsten Allers
Homologous recombination plays a central role in the repair of double-strand DNA breaks, the restart of stalled replication forks and the generation of genetic diversity. Regulation of recombination is essential since defects can lead to genome instability and chromosomal rearrangements. Strand exchange is a key step of recombination - it is catalysed by RecA in bacteria, Rad51/Dmc1 in eukaryotes and RadA in archaea. RadB, a paralogue of RadA, is present in many archaeal species. RadB has previously been proposed to function as a recombination mediator, assisting in RadA-mediated strand exchange...
April 26, 2017: DNA Repair
https://www.readbyqxmd.com/read/28499469/phylogenetically-diverse-groups-of-bradyrhizobium-isolated-from-nodules-of-tree-and-annual-legume-species-growing-in-ethiopia
#15
Tulu Degefu, Endalkachew Wolde-Meskel, Kedir Woliy, Åsa Frostegård
Bacteria belonging to the genus Bradyrhizobium nodulate various leguminous woody plants and herbs, including economically important crops such as soybean, peanut and cowpea. Here we analysed 39 Bradyrhizobium strains originating from root nodules of the leguminous trees and crops Acacia saligna, Faidherbia albida, Erythrina brucei, Albizia gummifera, Millettia ferruginea, Cajanus cajan, Vigna unguiculata and Phaseolus vulgaris, growing in southern Ethiopia. Multilocus sequence analyses (MLSA) of the 16S rRNA, glnII, recA, gyrB and dnaK genes and the ITS region grouped the test strains into seven well-supported genospecies (I-VII), six of which occupied distinct positions excluding all hitherto defined Bradyrhizobium species...
April 26, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28499096/genetic-and-phenotypic-diversity-of-rhizobia-nodulating-chickpea-cicer-arietinum-l-in-soils-from-southern-and-central-ethiopia
#16
Wondwosen Tena, Endalkachew Wolde-Meskel, Tulu Degefu, Fran Walley
Forty-two chickpea-nodulating rhizobia were isolated from soil samples collected from diverse agro-ecological locations of Ethiopia, and characterized based on 76 phenotypic traits. Furthermore, 18 representative strains were selected and characterized using multilocus sequence analyses (MLSA) of core and symbiotic gene loci. Numerical analysis of the phenotypic characteristics grouped the 42 strains in to four distinct clusters. The analysis of the 16S rRNA gene of the 18 strains showed that they belong to the Mesorhizobium genus...
May 12, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28492177/characterization-of-aliivibrio-fischeri-strains-associated-with-disease-outbreak-in-brill-scophthalmus-rhombus
#17
Jose R López, Laura Lorenzo, Rafael Alcantara, J I Navas
Three bacterial isolates were recovered from a disease outbreak with high mortality affecting brill Scophthalmus rhombus (Linnaeus, 1758). Moribund fish showed no external signs of disease, but plentiful haemorrhages were observed in liver. On the basis of phenotypic and genotypic characterization, the isolates were identified as Aliivibrio fischeri. The phenotypic profile of the isolates was basically similar to that of the type strain of this species, although some discrepancies were observed, mainly in the BIOLOG GN profile...
May 11, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28489851/global-analysis-of-double-strand-break-processing-reveals-in-vivo-properties-of-the-helicase-nuclease-complex-addab
#18
Anjana Badrinarayanan, Tung B K Le, Jan-Hendrik Spille, Ibrahim I Cisse, Michael T Laub
In bacteria, double-strand break (DSB) repair via homologous recombination is thought to be initiated through the bi-directional degradation and resection of DNA ends by a helicase-nuclease complex such as AddAB. The activity of AddAB has been well-studied in vitro, with translocation speeds between 400-2000 bp/s on linear DNA suggesting that a large section of DNA around a break site is processed for repair. However, the translocation rate and activity of AddAB in vivo is not known, and how AddAB is regulated to prevent excessive DNA degradation around a break site is unclear...
May 10, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28488863/v67l-mutation-fills-an-internal-cavity-to-stabilize-reca-mtu-intein
#19
Allison S Zwarycz, Martin Fossat, Otar Akanyeti, Zhongqian Lin, David J Rosenman, Angel E Garcia, Catherine A Royer, Kenneth V Mills, Chunyu Wang
Inteins mediate protein splicing, which has found extensive applications in protein science and biotechnology. In the Mycobacterium tuberculosis RecA mini-mini intein (ΔΔIhh), a single valine to leucine substitution at position 67 (V67L) dramatically increases intein stability and activity. However, crystal structures show that the V67L mutation causes minimal structural rearrangements, with a root-mean-square deviation of 0.2 Å between ΔΔIhh-V67 and ΔΔIhh-L67. Thus, the structural mechanisms for V67L stabilization and activation remain poorly understood...
May 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28486994/the-emergence-of-a-novel-sequence-type-of-mdr-acinetobacter-baumannii-from-the-intensive-care-unit-of-an-egyptian-tertiary-care-hospital
#20
Doaa Mohammad Ghaith, Mai Mahmoud Zafer, Mohamed Hamed Al-Agamy, Essam J Alyamani, Rayan Y Booq, Omar Almoazzamy
BACKGROUND AND AIM OF WORK: Acinetobacter baumannii is known for nosocomial outbreaks worldwide. In this study, we aimed to investigate the antibiotic susceptibility patterns and the clonal relationship of A. baumannii isolates from the intensive care unit (ICU) of an Egyptian hospital. METHODS: In the present study, 50 clinical isolates of multidrug resistant (MDR)-A. baumannii were obtained from patients admitted into the ICU from June to December 2015. All isolates were analyzed for antimicrobial susceptibilities...
May 10, 2017: Annals of Clinical Microbiology and Antimicrobials
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