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https://www.readbyqxmd.com/read/28712131/a-novel-antibacterial-peptide-derived-from-crocodylus-siamensis-hemoglobin-hydrolysate-induces-membrane-permeabilisation-causing-iron-dysregulation-oxidative-stress-and-bacterial-death
#1
J Lueangsakulthai, N Jangpromma, T Temsiripong, J E McKendrick, W Khunkitti, S E Maddocks, S Klaynongsruang
AIMS: A novel antibacterial peptide from Crocodylus siamensis hemoglobin hydrolysate (CHHs) was characterised for antimicrobial activity. METHODS AND RESULTS: CHHs was hydrolysed for 2 h (2h-CHH), 4 h (4h-CHH), 6 h (6h-CHH) and 8 h (8h-CHH). 8h-CHH showed antibacterial activity against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa at concentrations of 20, 20, 20 and 10 mg ml(-1) (w/v), respectively. Fluorescent microscopy revealed that 8h-CHH had bactericidal activity against E...
July 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28710290/3-terminated-overhangs-regulate-dna-double-strand-break-processing-in-escherichia-coli
#2
Edyta Đermić, Davor Zahradka, Dušica Vujaklija, Siniša Ivanković, Damir Đermić
Double-strand breaks (DSBs) are lethal DNA lesions, which are repaired by homologous recombination in Escherichia coli To study DSB processing in vivo, we induced DSBs into the E. coli chromosome by gamma irradiation and measured chromosomal degradation. We show that the DNA degradation is regulated by RecA protein concentration and its rate of association with ssDNA. RecA decreased DNA degradation in wild-type, recB and recD strains, indicating that it is a general phenomenon in E. coli On the other hand, DNA degradation was greatly reduced and unaffected by RecA in the recB1080 mutant (which produces long overhangs) and in a strain devoid of four exonucleases that degrade a 3' tail (ssExos)...
July 14, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28705667/clinical-characteristics-of-bacteraemia-caused-by-burkholderia-cepacia-complex-species-and-antimicrobial-susceptibility-of-the-isolates-in-a-medical-centre-in-taiwan
#3
Ying-Chun Chien, Chun-Hsing Liao, Wang-Huei Sheng, Jung-Yien Chien, Yu-Tsung Huang, Chong-Jen Yu, Po-Ren Hsueh
This study investigated the clinical characteristics and outcomes of bacteraemia due to Burkholderia cepacia complex (BCC) species among 54 patients without cystic fibrosis from January 2013 to February 2015. BCC isolates were identified to the species level by the Bruker Biotyper MALDI-TOF MS system and by sequencing analysis of the 16S rRNA and recA genes. Antimicrobial susceptibilities of the isolates were determined by the agar dilution method. Sequencing of the recA gene in the 54 blood isolates revealed 37 (68...
July 10, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28699877/isolation-and-characterization-of-kingella-negevensis-sp-nov-a-novel-kingella-species-detected-in-a-healthy-paediatric-population
#4
Nawal El Houmami, Sofiane Bakour, Janek Bzdrenga, Jaishiram Rathored, Hervé Seligmann, Catherine Robert, Nicholas Armstrong, Jacques Schrenzel, Didier Raoult, Pablo Yagupsky, Pierre-Edouard Fournier
We herein report the isolation and characterization of 21 Gram-stain-negative strains cultivated from the oropharynx of healthy children in Israel and Switzerland. Initially described as small colony variants of Kingella kingae, phenotypic analysis, biochemical analysis, phylogenetic analysis based on sequencing of the partial 16S rRNA gene and five housekeeping genes (abcZ, adk, G6PD, groEL and recA), and whole genome sequencing and comparison between members of the genera Kingella and Neisseria provided evidence for assigning them to the genus Kingella...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699873/rhizobium-zeae-sp-nov-isolated-from-maize-zea-mays-l-roots
#5
Lorena Celador-Lera, Esther Menéndez, Alvaro Peix, José M Igual, Encarna Velázquez, Raúl Rivas
A bacterial strain designated CRZM18RT was isolated from a root of Zea mays in Spain. The analysis of the 16S rRNA gene sequence showed that this strain belongs to the genus Rhizobium, with Rhizobium cellulosilyticum ALA10B2T and Rhizobium yantingense H66T being the most closely related species with 98.3 and 97.9 % sequence similarity, respectively. The analysis of the concatenated recA and atpD genes showed that strain CRZM18RT forms a cluster with these species and also with Rhizobiumsmilacinae PTYR-5T, but the recA and atpD genes of strain CRZM18RT were phylogenetically distant, with identities lower than 90 and 96 %, respectively...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28695409/selection-and-validation-of-reference-genes-for-rt-qpcr-indicates-that-juice-of-sugarcane-varieties-modulate-the-expression-of-c-metabolism-genes-in-the-endophytic-diazotrophic-herbaspirillum-rubrisubalbicans-strain-hcc103
#6
Valéria Polese, Cleiton de Paula Soares, Paula Renata Alves da Silva, Jean Luiz Simões-Araújo, José Ivo Baldani, Marcia Soares Vidal
Quantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources...
July 10, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28694800/bacterial-survival-under-extreme-uv-radiation-a-comparative-proteomics-study-of-rhodobacter-sp-isolated-from-high-altitude-wetlands-in-chile
#7
Vilma Pérez, Martha Hengst, Lenka Kurte, Cristina Dorador, Wade H Jeffrey, Ruddy Wattiez, Veronica Molina, Sabine Matallana-Surget
Salar de Huasco, defined as a polyextreme environment, is a high altitude saline wetland in the Chilean Altiplano (3800 m.a.s.l.), permanently exposed to the highest solar radiation doses registered in the world. We present here the first comparative proteomics study of a photoheterotrophic bacterium, Rhodobacter sp., isolated from this remote and hostile habitat. We developed an innovative experimental approach using different sources of radiation (in situ sunlight and UVB lamps), cut-off filters (Mylar, Lee filters) and a high-throughput, label-free quantitative proteomics method to comprehensively analyze the effect of seven spectral bands on protein regulation...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28693668/mesorhizobium-helmanticense-sp-nov-isolated-from-lotus-corniculatus-nodules
#8
Marta Marcos-García, Esther Menéndez, Marta Helena Ramírez-Bahena, Pedro F Mateos, Álvaro Peix, Encarna Velazquez, Raúl Rivas
In this study, three strains belonging to the genus Mesorhizobium, CSLC115NT, CSLC19N and CSLC37N, isolated from Lotus corniculatus nodules in Spain, were characterized. Their 16S rRNA gene sequences were closely related to those of Mesorhizobium metallidurans STM 2683T, Mesorhizobium tianshanense A-1BST, Mesorhizobium tarimense CCBAU 83306T, Mesorhizobium gobiense CCBAU 83330T and Mesorhizobium caraganae CCBAU 11299T with similarity values higher than 99.7 %. The analysis of concatenated recA and glnII genes showed that the most closely related type strains were M...
July 10, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28690592/assessment-of-multilocus-sequence-analysis-as-a-valuable-tool-for-the-classification-of-the-genus-salinivibrio
#9
Clara López-Hermoso, Rafael R de la Haba, Cristina Cristina Sánchez-Porro, R Thane Papke, Antonio Ventosa
The genus Salinivibrio includes obligatory halophilic bacteria and is commonly isolated from hypersaline habitats and salted food products. They grow optimally between 7.5 and 10% salts and are facultative anaerobes. Currently, this genus comprises four species, one of them, S. costicola, with three subspecies. In this study we isolated and characterized an additional 70 strains from solar salterns located in different locations. Comparative 16S rRNA gene sequence analysis identified these strains as belonging to the genus Salinivibrio but could not differentiate strains into species-like groups...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28689072/nucleolytic-degradation-of-3-ending-overhangs-is-essential-for-dna-end-resection-in-reca-loading-deficient-recb-mutants-of-escherichia-coli
#10
Siniša Ivanković, Dušica Vujaklija, Damir Đermić
Degradation of a 5'-ending strand is the hallmark of the universal process of DNA double strand break (DSB) resection, which results in creation of the central recombination intermediate, a 3'-ending overhang. Here we show that in Escherichia coli recB1080/recB1067 mutants, which are devoid of RecBCD's nuclease and RecA loading activities, degradation of the unwound 3' tail is as essential as is degradation of its 5'-ending complement. Namely, a synergistic action of ExoI, ExoVII, SbcCD and ExoX single-strand specific exonucleases (ssExos) of 3'-5' polarity was essential for preserving cell viability, DNA repair and homologous recombination in the recB1080/recB1067 mutants, to the same extent as the redundant action of 5'-tail trimming ssExos RecJ and ExoVII...
June 27, 2017: DNA Repair
https://www.readbyqxmd.com/read/28688831/local-chemical-sympathectomy-of-rat-bone-marrow-and-its-effect-on-marrow-cell-composition
#11
P Dubový, I Klusáková, L Kučera, J Osičková, J Chovancová, T Loja, J Mayer, M Doubek, M Joukal
Existing experimental studies of the effect of sympathetic nerve fibers on bone marrow cells are based on the systemic administration of neurotoxic 6-hydroxydopamine. The method of global chemical sympathectomy has some serious disadvantages and could lead to questionable results. We describe a new method of local chemical sympathectomy of rat femoral bone marrow using guanethidine (Ismelin) delivery using an osmotic mini pump. Local guanethidine treatment for 14days led to complete elimination of sympathetic fibers in femoral bone marrow in contrast to bone marrow of contralateral or naïve femurs...
June 23, 2017: Autonomic Neuroscience: Basic & Clinical
https://www.readbyqxmd.com/read/28683814/identification-and-characterization-of-burkholderia-multivorans-cca53
#12
Hironaga Akita, Zen-Ichiro Kimura, Mohd Zulkhairi Mohd Yusoff, Nobutaka Nakashima, Tamotsu Hoshino
OBJECTIVE: A lignin-degrading bacterium, Burkholderia sp. CCA53, was previously isolated from leaf soil. The purpose of this study was to determine phenotypic and biochemical features of Burkholderia sp. CCA53. RESULTS: Multilocus sequence typing (MLST) analysis based on fragments of the atpD, gltD, gyrB, lepA, recA and trpB gene sequences was performed to identify Burkholderia sp. CCA53. The MLST analysis revealed that Burkholderia sp. CCA53 was tightly clustered with B...
July 6, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28681809/an-outbreak-of-burkholderia-cepacia-complex-in-the-paediatric-unit-of-a-tertiary-care-hospital
#13
Swapna Mali, Lona Dash, Vikas Gautam, Jayanthi Shastri, Sunil Kumar
INTRODUCTION: Burkholderia cepacia complex (Bcc) has emerged as a serious nosocomial pathogen worldwide especially in patients with indwelling catheters and cystic fibrosis. Bcc is a common contaminant of pharmaceutical products. We describe an outbreak of Bcc bacteraemia amongst children admitted in Paediatric Intensive Care Unit (PICU) and paediatric ward at a tertiary care hospital, Mumbai, in Western India. MATERIALS AND METHODS: Blood culture samples from paediatric patients yielded growth of non-fermenting, oxidase positive, motile, Gram negative bacilli (NFGNB) (76/909) over a period of 8 months...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28671523/bradyrhizobium-cajani-sp-nov-isolated-from-nodules-of-cajanus-cajan
#14
Juan Araújo, José David Flores-Félix, José M Igual, Alvaro Peix, Fernando González-Andrés, César Antonio Díaz-Alcántara, Encarna Velázquez
Two slow-growing strains, AMBPC1010T and AMBPC1011, were isolated from nodules of Cajanus cajan in the Dominican Republic. 16S rRNA gene analysis placed these strains within the genus Bradyrhizobium, being phylogenetically equidistant to several species of this genus. Analysis of the recA and atpD genes showed that the strains isolated belong to a cluster containing the strains Bradyrhizobium ottawaense OO99T, 'Bradyrhizobium americanum' CMVU44 and Bradyrhizobium daqingense CCBAU 15774T, and presented similarity values lower than 96 % for both genes with respect to the strains nodulating C...
July 3, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28667173/a-time-for-promiscuity-in-a-eukaryotic-recombinase
#15
REVIEW
Maria Spies
The exchange of DNA strands between broken and intact molecules lies at the heart of fundamental cellular processes ranging from repairing DNA damage by homologous recombination to generation of genetic diversity during sexual reproduction. New work by Lee and colleagues utilizes the DNA curtain method, an elegant single-molecule technique, to demonstrate common and idiosyncratic features in the DNA strand exchange mechanisms of three RecA-family recombinases, bacterial RecA, and eukaryotic Rad51 and Dmc1 proteins...
June 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28662104/vibrio-aphrogenes-sp-nov-in-the-rumoiensis-clade-isolated-from-a-seaweed
#16
Mami Tanaka, Shoko Endo, Fumihito Kotake, Nurhidayu Al-Saari, A K M Rohul Amin, Gao Feng, Sayaka Mino, Hidetaka Doi, Yoshitoshi Ogura, Tetsuya Hayashi, Wataru Suda, Masahira Hattori, Isao Yumoto, Toko Sawabe, Tomoo Sawabe, Toshiyoshi Araki
A novel strain Vibrio aphrogenes sp. nov. strain CA-1004T isolated from the surface of seaweed collected on the coast of Mie Prefecture in 1994 [1] was characterized using polyphasic taxonomy including multilocus sequence analysis (MLSA) and a genome based comparison. Both phylogenetic analyses on the basis of 16S rRNA gene sequences and MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the strain could be placed in the Rumoiensis clade in the genus Vibrio...
2017: PloS One
https://www.readbyqxmd.com/read/28651051/molecular-precision-at-micrometer-length-scales-hierarchical-assembly-of-dna-protein-nanostructures
#17
Daniel Schiffels, Veronika A Szalai, J Alexander Liddle
Robust self-assembly across length scales is a ubiquitous feature of biological systems but remains challenging for synthetic structures. Taking a cue from biology-where disparate molecules work together to produce large, functional assemblies-we demonstrate how to engineer microscale structures with nanoscale features: Our self-assembly approach begins by using DNA polymerase to controllably create double-stranded DNA (dsDNA) sections on a single-stranded template. The single-stranded DNA (ssDNA) sections are then folded into a mechanically flexible skeleton by the origami method...
July 3, 2017: ACS Nano
https://www.readbyqxmd.com/read/28651017/crystal-structure-of-middle-east-respiratory-syndrome-coronavirus-helicase
#18
Wei Hao, Justyna Aleksandra Wojdyla, Rong Zhao, Ruiyun Han, Rajat Das, Ivan Zlatev, Muthiah Manoharan, Meitian Wang, Sheng Cui
Middle East respiratory syndrome coronavirus (MERS-CoV) remains a threat to public health worldwide; however, effective vaccine or drug against CoVs remains unavailable. CoV helicase is one of the three evolutionary most conserved proteins in nidoviruses, thus making it an important target for drug development. We report here the first structure of full-length coronavirus helicase, MERS-CoV nsp13. MERS-CoV helicase has multiple domains, including an N-terminal Cys/His rich domain (CH) with three zinc atoms, a beta-barrel domain and a C-terminal SF1 helicase core with two RecA-like subdomains...
June 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28650754/structural-changes-in-the-lower-extremities-in-boys-aged-7-to-12-years-who-engage-in-moderate-physical-activityan-observational-longitudinal-study
#19
Gabriel Gijon-Nogueron, Salvador Diaz-Miguel, Eva Lopezosa-Reca, Jose Antonio Cervera-Marin
BACKGROUND: Physical activity in children may provide health benefits. We sought to consider the practice of soccer as a possible major factor in the development of the lower limb. The study is based on 3-year data for a group of children who practice this sport. METHODS: For 3 years we monitored 53 children who practiced soccer 3 times a week and had engaged in 2 years of continuous sports activity. Their mean ± SD age was 8.49 ± 2.01 years in the first year...
May 2017: Journal of the American Podiatric Medical Association
https://www.readbyqxmd.com/read/28647304/phylogenies-of-symbiotic-genes-of-bradyrhizobium-symbionts-of-legumes-of-economic-and-environmental-importance-in-brazil-support-the-definition-of-the-new-symbiovars-pachyrhizi-and-sojae
#20
Jakeline Renata Marçon Delamuta, Pâmela Menna, Renan Augusto Ribeiro, Mariangela Hungria
Bradyrhizobium comprises most tropical symbiotic nitrogen-fixing strains, but the correlation between symbiotic and core genes with host specificity is still unclear. In this study, the phylogenies of the nodY/K and nifH genes of 45 Bradyrhizobium strains isolated from legumes of economic and environmental importance in Brazil (Arachis hypogaea, Acacia auriculiformis, Glycine max, Lespedeza striata, Lupinus albus, Stylosanthes sp. and Vigna unguiculata) were compared to 16S rRNA gene phylogeny and genetic diversity by rep-PCR...
June 7, 2017: Systematic and Applied Microbiology
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