P Acera Mateos, A J Sethi, A Ravindran, A Srivastava, K Woodward, S Mahmud, M Kanchi, M Guarnacci, J Xu, Z W S Yuen, Y Zhou, A Sneddon, W Hamilton, J Gao, L M Starrs, R Hayashi, V Wickramasinghe, K Zarnack, T Preiss, G Burgio, N Dehorter, N E Shirokikh, E Eyras
The epitranscriptome embodies many new and largely unexplored functions of RNA. A significant roadblock hindering progress in epitranscriptomics is the identification of more than one modification in individual transcript molecules. We address this with CHEUI (CH3 (methylation) Estimation Using Ionic current). CHEUI predicts N6-methyladenosine (m6A) and 5-methylcytosine (m5C) in individual molecules from the same sample, the stoichiometry at transcript reference sites, and differential methylation between any two conditions...
May 9, 2024: Nature Communications