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dental stem cells

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https://www.readbyqxmd.com/read/29663385/quaking-promotes-the-odontoblastic-differentiation-of-human-dental-pulp-stem-cells
#1
Shuchen Li, Chujiao Lin, Jie Zhang, Huangheng Tao, Huan Liu, Guohua Yuan, Zhi Chen
Odontoblastic differentiation of human dental pulp stem cells (hDPSCs) is essential for the formation of reparative dentin after dental caries or injury. Our previous studies have demonstrated that krüppel-like factor 4 (KLF4) is a critical transcription factor that promotes the odontoblastic differentiation of hDPSCs. Analysis of the microRNA binding sites within the 3'-UTR of KLF4 revealed that QKI, an RNA-binding protein, shared the most microRNAs with KLF4, presumably served as a "competent endogenous RNA (ceRNA)" with KLF4...
April 16, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29656462/kdm1a-regulated-the-osteo-dentinogenic-differentiation-process-of-the-stem-cells-of-the-apical-papilla-via-binding-with-plod2
#2
Lijun Wang, Haoqing Yang, Xiao Lin, Yangyang Cao, Peipei Gao, Ying Zheng, Zhipeng Fan
OBJECTIVES: Dental tissue-derived mesenchymal stem cells (MSCs)-mediated pulp-dentin regeneration is considered a potential approach for the regeneration of damaged teeth. Enhancing MSC-mediated pulp-dentin regeneration is based on an understanding of the molecular mechanisms underlying directed cell differentiation process. Histone demethylation enzyme, lysine demethylase 1A (KDM1A) can regulate the differentiation of some MSCs, but its role in dental tissue-derived MSCs is unclear. MATERIAL AND METHODS: We obtained SCAPs from immature teeth...
April 15, 2018: Cell Proliferation
https://www.readbyqxmd.com/read/29652608/immunomodulatory-role-of-stem-cell-from-human-exfoliated-deciduous-teeth-on-periodontal-regeneration
#3
Xianling Gao, Zongshan Shen, Meiliang Guan, Qiting Huang, Lingling Chen, Wei Qin, Xiaohu Ge, Haijia Chen, Yin Xiao, Zhengmei Lin
Periodontitis is initiated by the infection of periodontal bacteria and subsequent tissue inflammation due to immunoreaction, eventually leading to periodontal apparatus loss. Stem cells from human exfoliated deciduous teeth (SHEDs) have exhibited beneficial characteristics in dental tissue regeneration. However, the immunomodulatory functions of SHEDs have not been elucidated in the context of periodontitis treatment. In this study, we investigated the potential immunomodulatory effects of SHEDs on experimental periodontitis and demonstrated that multi-dose delivery of SHEDs led to periodontal tissue regeneration...
March 20, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29650947/hsa-let-7c-controls-the-committed-differentiation-of-igf-1-treated-mesenchymal-stem-cells-derived-from-dental-pulps-by-targeting-igf-1r-via-the-mapk-pathways
#4
Gen-Xia Liu, Shu Ma, Yao Li, Yan Yu, Yi-Xiang Zhou, Ya-Die Lu, Lin Jin, Zi-Lu Wang, Jin-Hua Yu
The putative tumor suppressor microRNA let-7c is extensively associated with the biological properties of cancer cells. However, the potential involvement of let-7c in the differentiation of mesenchymal stem cells has not been fully explored. In this study, we investigated the influence of hsa-let-7c (let-7c) on the proliferation and differentiation of human dental pulp-derived mesenchymal stem cells (DPMSCs) treated with insulin-like growth factor 1 (IGF-1) via flow cytometry, CCK-8 assays, alizarin red staining, real-time RT-PCR, and western blotting...
April 13, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29650250/thermo-setting-glass-ionomer-cements-promote-variable-biological-responses-of-human-dental-pulp-stem-cells
#5
Mar Collado-González, Miguel R Pecci-Lloret, Christopher J Tomás-Catalá, David García-Bernal, Ricardo E Oñate-Sánchez, Carmen Llena, Leopoldo Forner, Vinicius Rosa, Francisco J Rodríguez-Lozano
OBJECTIVE: To evaluate the in vitro cytotoxicity of Equia Forte (GC, Tokyo, Japan) and Ionostar Molar (Voco, Cuxhaven, Germany) on human dental pulp stem cells (hDPSCs). METHODS: hDPSCs isolated from third molars were exposed to several dilutions of Equia Forte and Ionostar Molar eluates (1/1, 1/2 and 1/4). These eluates were obtained by storing material samples in respective cell culture medium for 24h (n=40). hDPSCs in basal growth culture medium were the control...
April 9, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29650042/dental-pulp-stem-cells-used-to-deliver-the-anticancer-drug-paclitaxel
#6
Hamideh Salehi, Siham Al-Arag, Elodie Middendorp, Csilla Gergely, Frederic Cuisinier, Valerie Orti
BACKGROUND: Understanding stem cell behavior as a delivery tool in cancer therapy is essential for evaluating their future clinical potential. Previous in-vivo studies proved the use of mesenchymal stem cells (MSCs) for local delivery of the commonest anticancer drug, paclitaxel (PTX). Dental pulp is a relatively abundant noninvasive source of MSCs. We assess dental pulp stem cells (DPSCs), for the first time, as anticancer drug carriers. Confocal Raman microscopy is a unique tool to trace drug and cell viability without labeling...
April 12, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29644924/role-of-prion-protein-egfr-multimolecular-complex-during-neuronal-differentiation-of-human-dental-pulp-derived-stem-cells
#7
Stefano Martellucci, Valeria Manganelli, Costantino Santacroce, Francesca Santilli, Luca Piccoli, Maurizio Sorice, Vincenzo Mattei
Cellular prion protein (PrPC ) is expressed in a wide variety of stem cells in which regulates their self-renewal as well as differentiation potential. In this study we investigated the presence of PrPC in human dental pulp-derived stem cells (hDPSCs) and its role in neuronal differentiation process. We show that hDPSCs expresses early PrPC at low concentration and its expression increases after two weeks of treatment with EGF/bFGF. Then, we analyzed the association of PrPC with gangliosides and EGF receptor (EGF-R) during neuronal differentiation process...
April 12, 2018: Prion
https://www.readbyqxmd.com/read/29643528/-comparison-of-the-properties-of-cd146-positive-and-cd146-negative-subpopulations-of-stem-cells-from-human-exfoliated-deciduous-teeth
#8
X T Wang, N Q Rao, T Jz Fang, Y M Zhao, L H Ge
OBJECTIVE: Stem cells from human exfoliated teeth (SHED) were sorted by magnetically activated cell sorting (MACS) technique to obtain the CD146 positive and negative cell subpopulation. Then the biological characteristics of these subpopulations were compared to explore their specific application potential in tissue engineering. METHODS: In this study, freshly extracted deciduous teeth without any caries or dental pulp disease were obtained. SHED was isolated using enzyme digestion method and then sorted by MACS, CD146 positive cells and CD146 negative cells were obtained after cell sorting...
April 18, 2018: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29642566/in-vitro-and-in-vivo-osteogenic-activity-of-titanium-implants-coated-by-pulsed-laser-deposition-with-a-thin-film-of-fluoridated-hydroxyapatite
#9
Luyuan Chen, Satoshi Komasa, Yoshiya Hashimoto, Shigeki Hontsu, Joji Okazaki
To enhance biocompatibility, osteogenesis, and osseointegration, we coated titanium implants, by krypton fluoride (KrF) pulsed laser deposition, with a thin film of fluoridated hydroxyapatite (FHA). Coating was confirmed by scanning electron microscopy (SEM) and scanning probe microscopy (SPM), while physicochemical properties were evaluated by attenuated reflectance Fourier transform infrared spectroscopy (ATR-FTIR). Calcium deposition, osteocalcin production, and expression of osteoblast genes were significantly higher in rat bone marrow mesenchymal stem cells seeded on FHA-coated titanium than in cells seeded on uncoated titanium...
April 10, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29626259/hypoxia-upregulates-the-expression-of-the-pluripotency-markers-in-the-stem-cells-from-human-deciduous-teeth
#10
Stefanie Bressan Werle, Pedro Chagastelles, Patricia Pranke, Luciano Casagrande
OBJECTIVES: Cultivation under hypoxia promotes different responses in the mesenchymal stem cells and it has been producing promising results for clinical applications. Pulp tissue from deciduous teeth is a source of stem cells which has a high proliferative potential but this is usually discarded. This study has evaluated the effects of hypoxia on proliferation, apoptosis, and the expression of the pluripotency-related genes of the stem cells from human exfoliated deciduous teeth (SHED)...
April 7, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29620442/mir-21-stat3-signal-is-involved-in-odontoblast-differentiation-of-human-dental-pulp-stem-cells-mediated-by-tnf-%C3%AE
#11
Ke Xu, Jingwen Xiao, Ke Zheng, Xingmei Feng, Jinlong Zhang, Donghui Song, Chenfei Wang, Xiang Shen, Xin Zhao, Changbo Wei, Dan Huang, Guijuan Feng
Dental pulp stem cells (DPSCs), as one type of mesenchymal stem cells (MSCs), have the capability of self-renewal and multipotency to differentiate into several cell lineages, including osteogenesis, odontoblasts, chondrogenesis, neurogenesis, and adipogenesis. It has found that tumor necrosis factor-α (TNF-α) can promote osteogenic differentiation of human DPSCs in our previous studies. Other experimentation revealed that signal transducer and activator of transcription 3 (STAT3) underwent a rapid activation both in osteogenesis and inflammation microenvironment of MSCs in vitro...
April 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29606400/cell-homing-for-pulp-tissue-engineering-with-endogenous-dentin-matrix-proteins
#12
Matthias Widbiller, Ronald B Driesen, Andreas Eidt, Ivo Lambrichts, Karl-Anton Hiller, Wolfgang Buchalla, Gottfried Schmalz, Kerstin M Galler
INTRODUCTION: Compelling evidence pinpoints that pulp tissue engineering after the transplantation of stem cells is possible. Although intriguing, severe problems regarding clinical feasibility remain. Cell homing has been proposed as a viable alternative in which dentin-derived growth factors in a conducive scaffold may attract resident cells to form pulplike tissue. In this study, an ectopic animal model for in situ dental pulp tissue engineering was developed to evaluate whether pulplike tissue formation in empty root canals after the attraction of stem cells was possible and whether this could be enhanced by dentin-derived growth factors...
March 29, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29604386/applications-of-inflammation-derived-gingival-stem-cells-for-testing-the-biocompatibility-of-dental-restorative-biomaterials
#13
A Soancă, M Lupse, M Moldovan, E Pall, M Cenariu, A Roman, O Tudoran, P Surlin, O Șorițău
BACKGROUND: Normal or inflamed gingival tissues are regarded as a source of mesenchymal stem cells (MSCs) abundant and easily accessible through minimally invasive dental procedures. Due to the proximity of dental resin composites to gingival tissues and to the possible local cytotoxic effect of the eluted components, gingiva-derived MSCs could be used to investigate the biocompatibility of dental biomaterials. PURPOSE: The present research aimed to isolate (MSCs) from inflamed and normal gingiva, to fully characterize them and to observe their behavior in relation with some commercial resin composite materials and one experimental material...
March 28, 2018: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29590258/human-dental-pulp-stem-cell-adhesion-and-detachment-in-polycaprolactone-electrospun-scaffolds-under-direct-perfusion
#14
A Paim, D I Braghirolli, N S M Cardozo, P Pranke, I C Tessaro
Cell adhesion in three-dimensional scaffolds plays a key role in tissue development. However, stem cell behavior in electrospun scaffolds under perfusion is not fully understood. Thus, an investigation was made on the effect of flow rate and shear stress, adhesion time, and seeding density under direct perfusion in polycaprolactone electrospun scaffolds on human dental pulp stem cell detachment. Polycaprolactone scaffolds were electrospun using a solvent mixture of chloroform and methanol. The viable cell number was determined at each tested condition...
March 26, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29581460/loss-of-stemness-emt-and-supernumerary-tooth-formation-in-cebpb-runx2-murine-incisors
#15
Kazuyuki Saito, Katsu Takahashi, Boyen Huang, Masakazu Asahara, Honoka Kiso, Yumiko Togo, Hiroko Tsukamoto, Sayaka Mishima, Masaki Nagata, Machiko Iida, Yoshihito Tokita, Masato Asai, Akira Shimizu, Toshihisa Komori, Hidemitsu Harada, Mary MacDougall, Manabu Sugai, Kazuhisa Bessho
Adult Cebpb KO mice incisors present amelogenin-positive epithelium pearls, enamel and dentin allopathic hyperplasia, fewer Sox2-positive cells in labial cervical loop epitheliums, and reduced Sox2 expression in enamel epithelial stem cells. Thus, Cebpb acts upstream of Sox2 to regulate stemness. In this study, Cebpb KO mice demonstrated cementum-like hard tissue in dental pulp, loss of polarity by ameloblasts, enamel matrix in ameloblastic layer, and increased expression of epithelial-mesenchymal transition (EMT) markers in a Cebpb knockdown mouse enamel epithelial stem cell line...
March 26, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29576778/cytotoxicity-assessment-of-polyhydroxybutyrate-chitosan-nano-bioglass-nanofiber-scaffolds-by-stem-cells-from-human-exfoliated-deciduous-teeth-stem-cells-from-dental-pulp-of-exfoliated-deciduous-tooth
#16
Batool Hashemi-Beni, Maryam Khoroushi, Mohammad Reza Foroughi, Saeed Karbasi, Abbas Ali Khademi
Background: The aim of this study was to compare the cytotoxicity and the biocompatibility of three different nanofibers scaffolds after seeding of stem cells harvested from human deciduous dental pulp. Given the importance of scaffold and its features in tissue engineering, this study demonstrated the construction of polyhydroxybutyrate (PHB)/chitosan/nano-bioglass (nBG) nanocomposite scaffold using electrospinning method. Materials and Methods: This experimental study was conducted on normal exfoliated deciduous incisors obtained from 6-year-old to 11-year-old healthy children...
March 2018: Dental Research Journal
https://www.readbyqxmd.com/read/29576501/intrastriatal-transplantation-of-stem-cells-from-human-exfoliated-deciduous-teeth-reduces-motor-defects-in-parkinsonian-rats
#17
Nan Zhang, Xianjie Lu, Shichao Wu, Xueli Li, Jing Duan, Chao Chen, Wei Wang, Hao Song, Jiabei Tong, Sen Li, Yanming Liu, Xinjiang Kang, Xuexiang Wang, Fabin Han
BACKGROUND: This study explored the neural differentiation and therapeutic effects of stem cells from human exfoliated deciduous teeth (SHED) in a rat model of Parkinson's disease (PD). METHODS: The SHED were isolated from fresh dental pulp and were induced to differentiate to neurons and dopamine neurons by inhibiting similar mothers against dpp (SMAD) signaling with Noggin and increase conversion of dopamine neurons from SHED with CHIR99021, Sonic Hedgehog (SHH) and FGF8 in vitro...
March 22, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29565801/mesenchymal-stem-cells-of-dental-origin-for-inducing-tissue-regeneration-in-periodontitis-a-mini-review
#18
REVIEW
Beatriz Hernández-Monjaraz, Edelmiro Santiago-Osorio, Alberto Monroy-García, Edgar Ledesma-Martínez, Víctor Manuel Mendoza-Núñez
Periodontitis is a chronic disease that begins with a period of inflammation of the supporting tissues of the teeth table and then progresses, destroying the tissues until loss of the teeth occurs. The restoration of the damaged dental support apparatus is an extremely complex process due to the regeneration of the cementum, the periodontal ligament, and the alveolar bone. Conventional treatment relies on synthetic materials that fill defects and replace lost dental tissue, but these approaches are not substitutes for a real regeneration of tissue...
March 22, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29559008/amenable-epigenetic-traits-of-dental-pulp-stem-cells-underlie-high-capability-of-xeno-free-episomal-reprogramming
#19
Srijaya Thekkeparambil Chandrabose, Sandhya Sriram, Subha Subramanian, Shanshan Cheng, Wee Kiat Ong, Steve Rozen, Noor Hayaty Abu Kasim, Shigeki Sugii
BACKGROUND: While a shift towards non-viral and animal component-free methods of generating induced pluripotent stem (iPS) cells is preferred for safer clinical applications, there is still a shortage of reliable cell sources and protocols for efficient reprogramming. METHODS: Here, we show a robust episomal and xeno-free reprogramming strategy for human iPS generation from dental pulp stem cells (DPSCs) which renders good efficiency (0.19%) over a short time frame (13-18 days)...
March 20, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29556464/angiogenic-and-osteogenic-potentials-of-dental-stem-cells-in-bone-tissue-engineering
#20
REVIEW
Muhammad Fuad Hilmi Yusof, Wafa' Zahari, Siti Nurnasihah Md Hashim, Zul Faizuddin Osman, Hamshawagini Chandra, Thirumulu Ponnuraj Kannan, Khairul Bariah Ahmad Amin Noordin, Ahmad Azlina
Manipulation of dental stem cells (DSCs) using current technologies in tissue engineering unveil promising prospect in regenerative medicine. DSCs have shown to possess angiogenic and osteogenic potential in both in vivo and in vitro. Neural crest derived DSCs can successfully be isolated from various dental tissues, exploiting their intrinsic great differentiation potential. In this article, researcher team intent to review the characteristics of DSCs, with focus on their angiogenic and osteogenic differentiation lineage...
January 2018: Journal of Oral Biology and Craniofacial Research
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