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dental stem cells

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https://www.readbyqxmd.com/read/29240205/regenerative-treatment-of-periodontal-intrabony-defects-using-autologous-dental-pulp-stem-cells-a-1-year-follow-up-case-series
#1
Mario Aimetti, Francesco Ferrarotti, Mara Noemi Gamba, Marta Giraudi, Federica Romano
The present case series aimed to explore the potential clinical benefits of the application of dental pulp stem cells (DPSCs) in the regenerative treatment of deep intrabony defects. A total of 11 isolated intrabony defects in 11 chronic periodontitis patients were accessed with a minimally invasive flap and filled with DPSCs loaded on a collagen sponge. A tooth requiring extraction for impaction or malpositioning was used as an autologous source for DPSCs. An average clinical attachment level gain of 4.7 ± 1...
January 2018: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/29237486/transplantation-of-dental-pulp-stem-cells-improves-long-term-diabetic-polyneuropathy-together-with-improvement-of-nerve-morphometrical-evaluation
#2
Maiko Omi, Masaki Hata, Nobuhisa Nakamura, Megumi Miyabe, Shogo Ozawa, Hitoshi Nukada, Masami Tsukamoto, Kazunori Sango, Tatsuhito Himeno, Hideki Kamiya, Jiro Nakamura, Jun Takebe, Tatsuaki Matsubara, Keiko Naruse
BACKGROUND: Although previous reports have revealed the therapeutic potential of stem cell transplantation in diabetic polyneuropathy, the effects of cell transplantation on long-term diabetic polyneuropathy have not been investigated. In this study, we investigated whether the transplantation of dental pulp stem cells (DPSCs) ameliorated long-term diabetic polyneuropathy in streptozotocin (STZ)-induced diabetic rats. METHODS: Forty-eight weeks after STZ injection, we transplanted DPSCs into the unilateral hindlimb skeletal muscles...
December 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29235086/endothelial-expression-of-c-kit-and-cd68-in-dental-follicles-of-human-impacted-third-molars
#3
Mugurel Constantin Rusu, Aliona Rașcu, Mihai Dragomir Stoenescu
Periodontal tissue remnants of odontogenesis constitute the dental follicle (DF) which is actually considered a stem niche in adults. However, potentialities of local endothelia within this niche seem overlooked. We thus aimed at testing the endothelial cells expression of c-kit, the progenitor cells marker, and CD68, commonly regarded as a monocyte/macrophage marker, in human DFs. We performed an immunohistochemical study using these two markers which were applied on samples collected from ten adult patients...
December 13, 2017: Folia Morphologica (Warsz)
https://www.readbyqxmd.com/read/29229947/recombinant-human-osteopontin-expressed-in-nicotiana-benthamiana-stimulates-osteogenesis-related-genes-in-human-periodontal-ligament-cells
#4
Kaewta Rattanapisit, Supaniga Abdulheem, Daneeya Chaikeawkaew, Anchanee Kubera, Hugh S Mason, Julian K-C Ma, Prasit Pavasant, Waranyoo Phoolcharoen
Tissue engineering aims to utilise biologic mediators to facilitate tissue regeneration. Several recombinant proteins have potential to mediate induction of bone production, however, the high production cost of mammalian cell expression impedes patient access to such treatments. The aim of this study is to produce recombinant human osteopontin (hOPN) in plants for inducing dental bone regeneration. The expression host was Nicotiana benthamiana using a geminiviral vector for transient expression. OPN expression was confirmed by Western blot and ELISA, and OPN was purified using Ni affinity chromatography...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29227501/chondrogenic-potential-of-macroporous-biodegradable-cryogels-based-on-synthetic-poly-%C3%AE-amino-acids
#5
T Sedlačík, O K Acar, H Studenovská, I Kotelnikov, J Kučka, Z Konečná, T Zikmund, J Kaiser, G T Kose, F Rypáček
In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(α-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N5-(2-hydroxyethyl)-l-glutamine-stat-N5-(2-methacryloyl-oxy-ethyl)-l-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers...
December 11, 2017: Soft Matter
https://www.readbyqxmd.com/read/29226516/sost-an-lngfr-target-inhibits-the-osteogenic-differentiation-of-rat-ectomesenchymal-stem-cells
#6
Gang Li, Junyu Liu, Manzhu Zhao, Yingying Wang, Kun Yang, Chang Liu, Yong Xiao, Xiujie Wen, Luchuan Liu
OBJECTIVES: The aim of this study was to investigate whether sclerostin (SOST) regulates the osteogenic differentiation of rat ectomesenchymal stem cells (EMSCs) and whether SOST and low-affinity nerve growth factor receptor (LNGFR) regulate the osteogenic differentiation of EMSCs. MATERIALS AND METHODS: EMSCs were isolated from embryonic facial processes from an embryonic 12.5-day (E12.5d) pregnant Sprague-Dawley rat. LNGFR+ EMSCs and LNGFR- EMSCs were obtained by fluorescence-activated cell sorting and were subsequently induced to undergo osteogenic differentiation in vitro...
December 10, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29220331/regenerative-potential-of-secretome-from-dental-stem-cells-a-systematic-review-of-preclinical-studies
#7
Suleiman Alhaji Muhammad, Norshariza Nordin, Sharida Fakurazi
Injury to tissues is a major clinical challenge due to the limited regenerative capacity of endogenous cells. Stem cell therapy is evolving rapidly as an alternative for tissue regeneration. However, increasing evidence suggests that the regenerative ability of stem cells is mainly mediated by paracrine actions of secretome that are generally secreted by the cells. We aimed to systematically evaluate the efficacy of dental stem cell (DSC)-conditioned medium in in vivo animal models of various tissue defects...
December 8, 2017: Reviews in the Neurosciences
https://www.readbyqxmd.com/read/29217199/efficient-generation-of-transgene-and-feeder-free-induced-pluripotent-stem-cells-from-human-dental-mesenchymal-stem-cells-and-their-chemically-defined-differentiation-into-cardiomyocytes
#8
Xiaobing Tan, Qingli Dai, Tao Guo, Jingshu Xu, Qingyuan Dai
Advance in stem cell research resulted in several processes to generate induced pluripotent stem cells (iPSCs) from adult somatic cells. In our previous study, the reprogramming of iPSCs from human dental mesenchymal stem cells (MSCs) including SCAP and DPSCs, has been reported. Herein, safe iPSCs were reprogrammed from SCAP and DPSCs using non-integrating RNA virus vector, which is an RNA virus carrying no risk of altering host genome. DPSCs- and SCAP-derived iPSCs exhibited the characteristics of the classical morphology with human embryonic stem cells (hESCs) without integration of foreign genes, indicating the potential of their clinical application...
December 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29215714/photobiomodulation-of-mesenchymal-stem-cells-encapsulated-in-an-injectable-rhbmp4-loaded-hydrogel-directs-hard-tissue-bioengineering
#9
Ivana Márcia Alves Diniz, Ana Cláudia O Carreira, Carla Renata Sipert, Cindi Mie Uehara, Maria Stella Nunes Moreira, Laila Freire, Cibele Pelissari, Patrícia M Kossugue, Daniele Ribeiro de Araújo, Mari Cleide Sogayar, Márcia Martins Marques
Photobiomodulation (PBM) therapy displays relevant properties for tissue healing and regeneration, which may be of interest for the tissue engineering field. Here, we show that PBM is able to improve cell survival and to interact with recombinant human Bone Morphogenetic Protein 4 (rhBMP4) to direct and accelerate odonto/osteogenic differentiation of dental derived mesenchymal stem cells (MSCs). MSCs were encapsulated in an injectable and thermo-responsive cell carrier (Pluronic® F-127) loaded with rhBMP4 and then photoactivated...
December 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29215170/synthetic-light-curable-polymeric-materials-provide-a-supportive-niche-for-dental-pulp-stem-cells
#10
Kyle H Vining, Jacob C Scherba, Alaina M Bever, Morgan R Alexander, Adam D Celiz, David J Mooney
Dental disease annually affects billions of patients, and while regenerative dentistry aims to heal dental tissue after injury, existing polymeric restorative materials, or fillings, do not directly participate in the healing process in a bioinstructive manner. There is a need for restorative materials that can support native functions of dental pulp stem cells (DPSCs), which are capable of regenerating dentin. A polymer microarray formed from commercially available monomers to rapidly identify materials that support DPSC adhesion is used...
December 7, 2017: Advanced Materials
https://www.readbyqxmd.com/read/29208555/hollow-mesoporous-zirconia-delivery-system-for-biomineralization-precursors
#11
Xue-Qing Huang, Hong-Ye Yang, Tao Luo, Cui Huang, Franklin R Tay, Li-Na Niu
Strategies based on the combination of nanocarrier delivery systems and scaffolds provide bone tissue engineering scaffolds with multifunctional capability. Zirconia, a biocompatible ceramic commonly used in orthopedic and dental implants, was used to synthesize hollow mesoporous nanocapsules for loading, storage and sustained release of a novel polyamine-stabilized liquid precursor phase of amorphous calcium phosphate (PAH-ACP) for collagen biomineralization and bone marrow stromal cells osteoinduction. Hollow mesoporous zirconia (hmZrO2) nanocapsules loaded with biomimetic precursors exhibited pH-sensitive release capability and good biocompatibility...
December 2, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29208280/formulation-and-biological-actions-of-nano-bioglass-ceramic-particles-doped-with-calcarea-phosphorica-for-bone-tissue-engineering
#12
S Dinesh Kumar, K Mohamed Abudhahir, N Selvamurugan, S Vimalraj, R Murugesan, N Srinivasan, A Moorthi
The improvisation of the treatment procedures for treating the various kind of bone defects such as, bone or dental trauma and for diseases such as osteoporosis, osteomyelitis etc., need the suitable and promising biomaterials with resemblance of bone components. Bioactive glass ceramic (BGC) has recently acquired great attention as the most promising biomaterials; hence it has been widely applied as a filler material for bone tissue regeneration. Because it elicts specific biological responses after implantation in addition more potential in formation of strong interface with both hard and soft tissues by dissolution of calcium and phosphate ions...
February 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29207466/evaluation-of-poly-lactic-co-glycolic-acid-alone-or-in-combination-with-hydroxyapatite-on-human-periosteal-cells-bone-differentiation-and-in-sinus-lift-treatment
#13
Gabriele Ceccarelli, Rossella Presta, Saturnino Marco Lupi, Nefele Giarratana, Nora Bloise, Laura Benedetti, Maria Gabriella Cusella De Angelis, Ruggero Rodriguez Y Baena
Most recent advances in tissue engineering in the fields of oral surgery and dentistry have aimed to restore hard and soft tissues. Further improvement of these therapies may involve more biological approaches and the use of dental tissue stem cells in combination with inorganic/organic scaffolds. In this study, we analyzed the osteoconductivity of two different inorganic scaffolds based on poly (lactic-co-glycolic) acid alone (PLGA-Fisiograft) or in combination with hydroxyapatite (PLGA/HA-Alos) in comparison with an organic material based on equine collagen (PARASORB Sombrero) both in vitro and in vivo...
December 2, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29204250/generation-and-role-of-reactive-oxygen-and-nitrogen-species-induced-by-plasma-lasers-chemical-agents-and-other-systems-in-dentistry
#14
REVIEW
Nayansi Jha, Jae Jun Ryu, Eun Ha Choi, Nagendra Kumar Kaushik
The generation of reactive oxygen and nitrogen species (RONS) has been found to occur during inflammatory procedures, during cell ischemia, and in various crucial developmental processes such as cell differentiation and along cell signaling pathways. The most common sources of intracellular RONS are the mitochondrial electron transport system, NADH oxidase, and cytochrome P450. In this review, we analyzed the extracellular and intracellular sources of reactive species, their cell signaling pathways, the mechanisms of action, and their positive and negative effects in the dental field...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29201060/osteogenic-differentiation-of-hdpscs-on-biogenic-bone-apatite-thin-films
#15
Michele Bianchi, Alessandra Pisciotta, Laura Bertoni, Matteo Berni, Alessandro Gambardella, Andrea Visani, Alessandro Russo, Anto de Pol, Gianluca Carnevale
A previous study reported the structural characterization of biogenic apatite (BAp) thin films realized by a pulsed electron deposition system by ablation of deproteinized bovine bone. Thin films annealed at 400°C exhibited composition and crystallinity degree very close to those of biogenic apatite; this affinity is crucial for obtaining faster osseointegration compared to conventional, thick hydroxyapatite (HA) coatings, for both orthopedics and dentistry. Here, we investigated the adhesion, proliferation, and osteogenic differentiation of human dental pulp stem cells (hDPCS) on as-deposited and heat-treated BAp and stoichiometric HA...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29201058/comparing-circadian-dynamics-in-primary-derived-stem-cells-from-different-sources-of-human-adult-tissue
#16
Eve H Rogers, Sandra A Fawcett, Vanja Pekovic-Vaughan, John A Hunt
Optimising cell/tissue constructs so that they can be successfully accepted and integrated within a host body is essential in modern tissue engineering. To do this, adult stem cells are frequently utilised, but there are many aspects of their environment in vivo that are not completely understood. There is evidence to suggest that circadian rhythms and daily circadian temporal cues have substantial effects on stem cell activation, cell cycle, and differentiation. It was hypothesised that the circadian rhythm in human adult stem cells differs depending on the source of tissue and that different entraining signals exert differential effects depending on the anatomical source...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29200956/stem-cells-from-cryopreserved-human-dental-pulp-tissues-sequentially-differentiate-into-definitive-endoderm-and-hepatocyte-like-cells-in-vitro
#17
Young-Jin Han, Young-Hoon Kang, Sarath Belame Shivakumar, Dinesh Bharti, Young-Bum Son, Yong-Ho Choi, Won-Uk Park, June-Ho Byun, Gyu-Jin Rho, Bong-Wook Park
We previously described a novel tissue cryopreservation protocol to enable the safe preservation of various autologous stem cell sources. The present study characterized the stem cells derived from long-term cryopreserved dental pulp tissues (hDPSCs-cryo) and analyzed their differentiation into definitive endoderm (DE) and hepatocyte-like cells (HLCs) in vitro. Human dental pulp tissues from extracted wisdom teeth were cryopreserved as per a slow freezing tissue cryopreservation protocol for at least a year...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29199334/-effect-of-transfected-ephb4-on-osteogenic-differentiation-in-stem-cells-from-human-exfoliated-deciduous-teeth
#18
Xiao-Ming Li, Chang-Yong Yuan, Shao-Yue Zhu, Zong-Xiang Liu, Peng-Lai Wang
PURPOSE: To evaluate the effect of stem cells from human exfoliated deciduous teeth (SHED) transfected with EphB4 gene in regulating osteogenic differentiation. METHODS: Human dental pulp tissue were harvested from extracted deciduous teeth and digested by collagenase and dispase. The SHEDs were transfected with transgenic (hEphB4-GFP) vector or empty vector (GFP-vector). Real time-polymerase chain reaction(real time-PCR) analysis and Western blot were used to detect the expression of EphB4 in SHEDs after transfection...
August 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29199326/-effect-of-glud1-on-proliferation-osteogenic-differentiation-and-mineralization-of-human-dental-pulp-stem-cells
#19
Xi Lu, Shang-Feng Liu, Shou-Liang Zhao
PURPOSE: To investigate the effect of glutamate dehydrogenase 1 (GLUD1) on proliferation, osteogenic differentiation and mineralization of human dental pulp stem cells (hDPSCs). METHODS: hDPSCs were isolated by tissue-explant method in vitro, and shGLUD1 lentivirus was transfected to knock down the expression of GLUD1. RT-PCR and Western blot were performed to detect the expression of GLUD1. CCK8 assay was used to evaluate cell proliferation. After culture with osteogenic inducing medium for 14 days, alizarin red staining was used to detect the formation of mineralization nodules, and RT-PCR and immunofluorescence staining were performed to detect the expression of Runx2 and OCN, respectively...
August 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29197101/a-biocompatible-decellularised-pulp-scaffold-for-regenerative-endodontics
#20
M Matoug-Elwerfelli, M S Duggal, H Nazzal, F Esteves, E Raïf
AIM: To investigate the feasibility of decellularising the entire dental pulp using a mild treatment protocol to develop a decellularised biological extracellular matrix scaffold for use in regenerative endodontic procedures. METHODOLOGY: Decellularised human dental pulps were assessed using histological and immunohistochemical methods, scanning electron microscope and DNA quantification assay. Cytotoxicity assays to determine decellularised scaffold biocompatibility were also performed...
December 1, 2017: International Endodontic Journal
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