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dental stem cells

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https://www.readbyqxmd.com/read/29035721/cdc42-is-essential-for-the-polarized-movement-and-adhesion-of-human-dental-pulp-stem-cells
#1
Mingwei Li, Liang Ma, Bing Song, Dingyi Yu, Min Xiao, Xiaohan Mei, Huihui Guo, Qing Yu
OBJECTIVE: Stem cell-based tissue repair and regeneration require the regulation of cell migration and adhesion. As a regulator of cell polarization, Cdc42 (cell division control protein 42) plays a basic role at the initial stage of cell migration and adhesion. This study explores the effect of Cdc42 on the polarized migration and adhesion of hDPSCs (human dental pulp stem cells). DESIGN: HDPSCs were isolated from extracted third molars and transfected with siRNA targeted against Cdc42...
October 3, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29033090/the-antimicrobial-peptide-ll-37-as-a-possible-adjunct-for-the-proliferation-and-differentiation-of-dental-pulp-stem-cells
#2
Noala Vicensoto Moreira Milhan, Patricia Pimentel de Barros, Elis Andrade de Lima Zutin, Felipe Eduardo de Oliveira, Carlos Henrique Ribeiro Camargo, Samira Esteves Afonso Camargo
INTRODUCTION: This study evaluated the biocompatibility of 5 and 10 μg/mL LL-37 in vitro and its effect on the differentiation of human dental pulp stem cells (DPSCs) into odontoblast-like cells. METHODS: Cell viability, genotoxicity, nitric oxide production, cell cycle, dentine sialophosphoprotein (DSPP) production, and DSPP gene expression. RESULTS: Concentrations of 5 and 10 μg/mL of LL-37 were not cytotoxic and generally increased cell viability, especially on the third day (P < ...
October 12, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/29032813/light-cured-tricalcium-silicate-toxicity-to-the-dental-pulp
#3
Charlotte Jeanneau, Patrick Laurent, Charlotte Rombouts, Thomas Giraud, Imad About
INTRODUCTION: Numerous studies reported dentin bridge formation after pulp capping with tricalcium silicates. By contrast, pulp capping with resins leads to pulp toxicity and inflammation. Hybrid materials made up of tricalcium silicates and resins have also been developed to be used in direct pulp capping. This work was designed to study the consequences of adding resins to tricalcium silicates by investigating TheraCal (BISCO, Lançon De Provence, France) and Biodentine (Septodont, Saint Maur des Fosses, France) interactions with the dental pulp...
October 9, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/29031235/homeobox-genes-and-tooth-development-understanding-the-biological-pathways-and-applications-in-regenerative-dental-science
#4
REVIEW
Anand Ramanathan, Thekkeparambil Chandrabose Srijaya, Prema Sukumaran, Rosnah Binti Zain, Noor Hayaty Abu Kasim
OBJECTIVES: Homeobox genes are a group of conserved class of transcription factors that function as key regulators during the embryonic developmental processes. They act as master regulator for developmental genes, which involves coordinated actions of various auto and cross-regulatory mechanisms. In this review, we summarize the expression pattern of homeobox genes in relation to the tooth development and various signaling pathways or molecules contributing to the specific actions of these genes in the regulation of odontogenesis...
October 4, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29026303/delivery-of-antagomir204-conjugated-gold-nanoparticles-from-plga-sheets-and-its-implication-in-promoting-osseointegration-of-titanium-implant-in-type-2-diabetes-mellitus
#5
Xiangwei Liu, Naiwen Tan, Yuchao Zhou, Hongbo Wei, Shuai Ren, Fan Yu, Hui Chen, Chengming Jia, Guodong Yang, Yingliang Song
Impaired osseointegration of the implant remains the big hurdle for dental implant therapy in diabetic patients. In this study, the authors first identified that miR204 was strikingly highly expressed in the bone mesenchymal stem cells (BMSCs) of diabetic rats. Forced expression of miR204 repressed the osteogenic potential of BMSCs, while inhibition of miR204 significantly increased the osteogenic capacity. Moreover, the miR204 inhibitor was conjugated with gold nanoparticles (AuNP-antagomiR204) and dispersed them in the poly(lactic-co-glycolic acid) (PLGA) solution...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29023390/electrospun-zein-gelatin-scaffold-enhanced-cell-attachment-and-growth-of-human-periodontal-ligament-stem-cells
#6
Fanqiao Yang, Yingling Miao, Yan Wang, Li-Ming Zhang, Xuefeng Lin
Periodontitis is a widespread dental disease affecting 10 to 15% of worldwide adult population, yet the current treatments are far from satisfactory. The human periodontal ligament stem cell is a promising potential seed cell population type in cell-based therapy and tissue regeneration, which require appropriate scaffold to provide a mimic extracellular matrix. Zein, a native protein derived from corn, has an excellent biodegradability, and therefore becomes a hotspot on research and application in the field of biomaterials...
October 12, 2017: Materials
https://www.readbyqxmd.com/read/29020731/clinical-risk-factors-influencing-dental-developmental-disturbances-in-childhood-cancer-survivors
#7
Chung-Min Kang, Seung Min Hahn, Hyo Sun Kim, Chuhl Joo Lyu, Jae-Ho Lee, Jinae Lee, Jung Woo Han
Purpose: Although studies regarding dental developmental disturbances after childhood cancer treatment have increased, they have many limitations. Studies analyzing the significance of independent clinical risk factors with regard to the dental health status are also rare. We aimed to investigate the risk factors for dental developmental disturbances, particularly severe disturbances, in childhood cancer survivors (CCS). Materials and Methods: Oral examinations and retrospective reviews of medical and panoramic radiographs were performed for 196 CCS (mean age, 15...
October 10, 2017: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/29018483/the-angiogenic-potential-of-dpscs-and-scaps-in-an-in-vivo-model-of-dental-pulp-regeneration
#8
Petra Hilkens, Annelies Bronckaers, Jessica Ratajczak, Pascal Gervois, Esther Wolfs, Ivo Lambrichts
Adequate vascularization, a restricting factor for the survival of engineered tissues, is often promoted by the addition of stem cells or the appropriate angiogenic growth factors. In this study, human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAPs) were applied in an in vivo model of dental pulp regeneration in order to compare their regenerative potential and confirm their previously demonstrated paracrine angiogenic properties. 3D-printed hydroxyapatite scaffolds containing DPSCs and/or SCAPs were subcutaneously transplanted into immunocompromised mice...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28991390/magnetically-controlled-growth-factor-immobilized-multilayer-cell-sheets-for-complex-tissue-regeneration
#9
Wenjie Zhang, Guangzheng Yang, Xiansong Wang, Liting Jiang, Fei Jiang, Guanglong Li, Zhiyuan Zhang, Xinquan Jiang
The scaffold-free cell-sheet technique plays a significant role in stem-cell-based regeneration. Furthermore, growth factors are known to direct stem cell differentiation and enhance tissue regeneration. However, the absence of an effective means to incorporate growth factors into the cell sheets hinders further optimization of the regeneration efficiency. Here, a novel design of magnetically controlled "growth-factor-immobilized cell sheets" is reported. A new Fe3 O4 magnetic nanoparticle (MNP) coated with nanoscale graphene oxide (nGO@Fe3 O4 ) is developed to label stem cells and deliver growth factors...
October 9, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28974767/the-neurotrophic-effects-of-different-human-dental-mesenchymal-stem-cells
#10
Mallappa K Kolar, Vinay N Itte, Paul J Kingham, Lev N Novikov, Mikael Wiberg, Peyman Kelk
The current gold standard treatment for peripheral nerve injury is nerve grafting but this has disadvantages such as donor site morbidity. New techniques focus on replacing these grafts with nerve conduits enhanced with growth factors and/or various cell types such as mesenchymal stem cells (MSCs). Dental-MSCs (D-MSCs) including stem cells obtained from apical papilla (SCAP), dental pulp stem cells (DPSC), and periodontal ligament stem cells (PDLSC) are potential sources of MSCs for nerve repair. Here we present the characterization of various D-MSCs from the same human donors for peripheral nerve regeneration...
October 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28972822/inhibition-of-tgf-%C3%AE-signaling-in-shed-enhances-endothelial-differentiation
#11
J G Xu, T Gong, Y Y Wang, T Zou, B C Heng, Y Q Yang, C F Zhang
Low efficiency of deriving endothelial cells (ECs) from adult stem cells hampers their utilization in tissue engineering studies. The purpose of this study was to investigate whether suppression of transforming growth factor beta (TGF-β) signaling could enhance the differentiation efficiency of dental pulp-derived stem cells into ECs. We initially used vascular endothelial growth factor A (VEGF-A) to stimulate 2 dental pulp-derived stem cells (dental pulp stem cells and stem cells from human exfoliated deciduous teeth [SHED]) and compared their differentiation capacity into ECs...
October 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28967188/bioactive-nano-fibrous-scaffold-for-vascularized-craniofacial-bone-regeneration
#12
Rahul Damodaran Prabha, David Christian Evar Kraft, Linda Harkness, Birte Melsen, Harikrishna Varma, Prabha D Nair, Jorgen Kjems, Mousthapha Kassem
There has been a growing demand for bone grafts for correction of bone defects in complicated fractures or tumors in the craniofacial region. Soft flexible membrane like material that could be inserted into defect by less invasive approaches; promote osteoconductivity and act as a barrier to soft tissue in growth while promoting bone formation is an attractive option for this region. Electrospinning has recently emerged as one of the most promising techniques for fabrication of extracellular matrix (ECM) like nano-fibrous scaffolds that can serve as a template for bone formation...
October 2, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28962902/the-role-of-vasculature-engineering-in-dental-pulp-regeneration
#13
Waruna Lakmal Dissanayaka, Chengfei Zhang
Creating an optimal microenvironment that supports angiogenesis, cell-cell cross talk, cell migration, and differentiation is crucial for pulp/dentin regeneration. It was shown that dental stem cells being seeded onto a scaffold and transplanted in vivo could give rise to a new tissue similar to that of the native pulp. However, the unique structure of the tooth with a pulp space encased within hard dentin allows only a single blood supply from a small apical opening located at the apex of the root canals...
September 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28962660/local-application-of-igfbp5-protein-enhanced-periodontal-tissue-regeneration-via-increasing-the-migration-cell-proliferation-and-osteo-dentinogenic-differentiation-of-mesenchymal-stem-cells-in-an-inflammatory-niche
#14
Nannan Han, Fengqiu Zhang, Guoqing Li, Xiuli Zhang, Xiao Lin, Haoqing Yang, Lijun Wang, Yangyang Cao, Juan Du, Zhipeng Fan
BACKGROUND: Periodontitis is a widespread infectious disease ultimately resulting in tooth loss. The number of mesenchymal stem cells (MSCs) in patients with periodontitis is decreased, and MSC functions are impaired. Rescuing the impaired function of MSCs in periodontitis is the key for treatment, especially in a manner independent of exogenous MSCs. Our previous study found that overexpressed insulin-like growth factor binding protein 5 (IGFBP5) could promote exogenous MSC-mediated periodontal tissue regeneration...
September 29, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28962641/overexpression-of-hypoxia-inducible-factor-1-alpha-improves-immunomodulation-by-dental-mesenchymal-stem-cells
#15
Victor G Martinez, Imelda Ontoria-Oviedo, Carolina P Ricardo, Sian E Harding, Rosa Sacedon, Alberto Varas, Agustin Zapata, Pilar Sepulveda, Angeles Vicente
BACKGROUND: Human dental mesenchymal stem cells (MSCs) are considered as highly accessible and attractive MSCs for use in regenerative medicine, yet some of their features are not as well characterized as other MSCs. Hypoxia-preconditioning and hypoxia-inducible factor 1 (HIF-1) alpha overexpression significantly improves MSC therapeutics, but the mechanisms involved are not fully understood. In the present study, we characterize immunomodulatory properties of dental MSCs and determine changes in their ability to modulate adaptive and innate immune populations after HIF-1 alpha overexpression...
September 29, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28957734/simplified-conditions-for-storing-and-cryopreservation-of-dental-pulp-stem-cells
#16
Nam Cong-Nhat Huynh, Son Hoang Le, Vu Nguyen Doan, Lan Thi Quynh Ngo, Ha Le Bao Tran
OBJECTIVES: This study aimed to simplify the collection, isolation and cryopreservation procedure of human dental pulp stem cells (DPSCs) to ease the establishment of dental stem cell banking. DESIGN: Extracted third molars were collected and stored either in growth medium or in gentamicin-saline (480μg/ml) for 6, 9 or 12h. DPSCs were isolated and subjected to cryopreservation by a controlled-rate or rapid freezing method in 5 or 10% DMSO. Flow cytometry and growth pattern of DPSCs before and after cryopreservation were conducted...
September 7, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28955974/role-of-miz-1-in-amelx-gene-expression
#17
Hee-Jin Noh, Dong-In Koh, Kon-O Lee, Bu-Nam Jeon, Min-Kyeong Kim, Malcom L Snead, Man-Wook Hur
Amelogenin (AMELX) is the main component of the developing tooth enamel matrix and is essential for enamel thickness and structure. However, little is known about its transcriptional regulation. Using gene expression analysis, we found that MIZ-1, a potent transcriptional activator of CDKN1A, is expressed during odontoblastic differentiation of hDPSCs (human dental pulp stem cells), and is essential for odontoblast differentiation and mineralization. We also investigated how MIZ-1 regulates gene expression of AMELX...
December 2016: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/28955954/lgr4-is-required-for-sequential-molar-development
#18
Yukiko Yamakami, Koki Kohashi, Kazunori Oyama, Yasuaki Mohri, Shizu Hidema, Katsuhiko Nishimori
Tooth development requires proliferation, differentiation, and specific migration of dental epithelial cells, through well-organized signaling interactions with mesenchymal cells. Recently, it has been reported that leucine-rich repeat-containing G protein coupled receptor 4 (LGR4), the receptor of R-spondins, is expressed in many epithelial cells in various organs and tissues and is essential for organ development and stem cell maintenance. Here, we report that LGR4 contributes to the sequential development of molars in mice...
December 2016: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/28951745/in-vivo-articular-cartilage-regeneration-using-human-dental-pulp-stem-cells-cultured-in-an-alginate-scaffold-a-preliminary-study
#19
Manuel Mata, Lara Milian, Maria Oliver, Javier Zurriaga, Maria Sancho-Tello, Jose Javier Martin de Llano, Carmen Carda
Osteoarthritis is an inflammatory disease in which all joint-related elements, articular cartilage in particular, are affected. The poor regeneration capacity of this tissue together with the lack of pharmacological treatment has led to the development of regenerative medicine methodologies including microfracture and autologous chondrocyte implantation (ACI). The effectiveness of ACI has been shown in vitro and in vivo, but the use of other cell types, including bone marrow and adipose-derived mesenchymal stem cells, is necessary because of the poor proliferation rate of isolated articular chondrocytes...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28950723/transplantation-of-hgf-modified-dental-pulp-stem-cells-prevents-bone-loss-in-the-early-phase-of-ovariectomy-induced-osteoporosis
#20
Fanxuan Kong, Xuefeng Shi, FengJun Xiao, Yuefeng Yang, Xiaoyan Zhang, Li-Sheng Wang, Chu-Tse Wu, Hua Wang
Investigations based on mesenchymal stem cells (MSCs) for osteoporosis have been obtained intensive attention recently. MSCs can derive from various tissues, such as bone marrow, adipose, umbilical cord, placenta, dental pulp, etc. Among them, dental pulp derived MSCs (DPSCs) and hepatocyte growth factor (HGF)-modified DPSCs (DPSCs-HGF) highly expressed osteogenic related genes and have stronger osteogenic differentiation capacities. DPSCs have more benefits in treating osteoporosis. The purpose of this study is to investigate the roles of HGF gene modified DPSCs in bone regeneration using a mouse model of ovariectomy (OVX)-induced bone loss...
September 26, 2017: Human Gene Therapy
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