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dental stem cells

Beatriz Hernández-Monjaraz, Edelmiro Santiago-Osorio, Edgar Ledesma-Martínez, Andrés Alcauter-Zavala, Víctor Manuel Mendoza-Núñez
Objective To report a case of successful allogeneic grafting of mesenchymal dental pulp stem cells (DPSCs) as preliminary findings in a patient with periodontal disease enrolled into clinical trial ISRCTN12831118. Methods Mesenchymal stem cells from the dental pulp of a deciduous tooth from a 7-year-old donor were separated from the pulp chamber and processed via enzymatic digestion and centrifugation. DPSCs were passaged and cultured on a 35 × 13 mm culture dish in minimum essential medium-alpha, without supplementation...
January 1, 2018: Journal of International Medical Research
Leopoldina D F Almeida, Pedro S Babo, Cristiana R Silva, Márcia T Rodrigues, Josimeri Hebling, Rui L Reis, Manuela E Gomes
The restoration of dentine-pulp complex remains a challenge for dentists; nonetheless, it has been poorly addressed. An ideal system should modulate the host response, as well as enable the recruitment, proliferation and differentiation of relevant progenitor cells. Herein was proposed a photocrosslinkable hydrogel system based on hyaluronic acid (HA) and platelet lysate (PL). PL is a cocktail of growth factors (GFs) and cytokines involved in wound healing orchestration, obtained by the cryogenic processing of platelet concentrates, and was expected to provide the HA hydrogels specific biochemical cues to enhance pulp cells' recruitment, proliferation and differentiation...
June 14, 2018: Journal of Materials Science. Materials in Medicine
Phillip A Cleves, James C Hart, Rachel M Agoglia, Monica T Jimenez, Priscilla A Erickson, Linda Gai, Craig T Miller
Threespine stickleback fish offer a powerful system to dissect the genetic basis of morphological evolution in nature. Marine sticklebacks have repeatedly invaded and adapted to numerous freshwater environments throughout the Northern hemisphere. In response to new diets in freshwater habitats, changes in craniofacial morphology, including heritable increases in tooth number, have evolved in derived freshwater populations. Using a combination of quantitative genetics and genome resequencing, here we fine-mapped a quantitative trait locus (QTL) regulating evolved tooth gain to a cluster of ten QTL-associated single nucleotide variants, all within intron four of Bone Morphogenetic Protein 6 (Bmp6)...
June 14, 2018: PLoS Genetics
Jelena Simonovic, Bosko Toljic, Nadja Nikolic, Jasna Vujin, Radmila Panajotovic, Rados Gajic, Elena Bekyarova, Amelia Cataldi, Vladimir Parpura, Jelena Milasin
Stem cell-based therapies are considered a promising treatment modality for many medical conditions. Several types of stem cells with variable differentiation potentials have been isolated from dental tissues, among them stem cells from apical papilla (SCAP). In parallel, new classes of biocompatible nanomaterials have also been developed, including graphene and carbon nanotube-based materials. The aim of the study was to assess whether graphene dispersion (GD) and water-soluble single walled carbon nanotubes (ws-SWCNT), may enhance SCAPs capacity to undergo neural differentiation...
June 12, 2018: Journal of Biomedical Materials Research. Part A
Alessandra Pisciotta, Laura Bertoni, Massimo Riccio, Jonathan Mapelli, Albertino Bigiani, Marcella La Noce, Monia Orciani, Anto de Pol, Gianluca Carnevale
Human dental pulp is considered an interesting source of adult stem cells, due to the low-invasive isolation procedures, high content of stem cells and its peculiar embryological origin from neural crest. Based on our previous findings, a dental pulp stem cells sub-population, enriched for the expression of STRO-1, c-Kit, and CD34, showed a higher neural commitment. However, their biological properties were compromised when cells were cultured in adherent standard conditions. The aim of this study was to evaluate the ability of three dimensional floating spheres to preserve embryological and biological properties of this sub-population...
2018: Frontiers in Physiology
Chun-Chieh Huang, Raghuvaran Narayanan, Noah Warshawsky, Sriram Ravindran
Dental pulp is a highly vascularized and innervated tissue that provides sensitivity and vitality to the tooth. Chronic caries results in an infected pulp tissue prone to necrosis. Existing clinical treatments replace the living pulp tissue with a non-responsive resin filling resulting in loss of tooth vitality. Tissue engineering approaches to dental pulp tissue regeneration have been investigated to preserve tooth vitality and function. However, a critical criterion is the choice of growth factors that may promote mesenchymal stem cell differentiation and more importantly, vascularization...
2018: Frontiers in Physiology
J Q Ling, J Mao
Recently, various levels of success have been achieved in dental pulp and dentin regeneration using latest techniques such as pulp revascularization, stem cell transplantation and cell homing. These cutting-edge technologies utilize stem cell mobilization, homing and directional differentiation, stem cell sub-population isolation, expansion and transplantation, modification and optimization of biomaterials and/or synergetic effects of biological cues. The objective of this review is to identify approaches for clinical translation and improve the success rates of pulp regeneration...
June 9, 2018: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
Yessenia Valverde, Raghuvaran Narayanan, Satish B Alapati, Fanny Chmilewsky, Chun-Chieh Huang, Sriram Ravindran, Seung H Chung
INTRODUCTION: The nuclear enzyme poly(adenosine phosphate ribose) polymerase 1 (PARP-1) has been implicated in the maintenance and differentiation of several stem cells. The role of PARP-1 in dental pulp stem cell (DPSC) differentiation, especially in the context of its ability to modulate nerve regeneration factors, has not been investigated. Regeneration of neuronal components in pulp tissue is important for the assessment of tooth vitality. Brain-derived neurotrophic factor (BDNF) is known to play an integral signaling factor during nerve regeneration...
June 5, 2018: Journal of Endodontics
Dean Whiting, Whasun Oh Chung, James D Johnson, Avina Paranjpe
INTRODUCTION: Dental stem cells have gained importance recently and are being used for various purposes in regenerative medicine and dentistry. Although much research has been done to show the various properties of these dental stem cells, the immunomodulatory properties of some of these stem cells are still unknown. This is important considering these cells are being used routinely. Therefore, the aim of this study was to investigate the interactions between the activated immune cells and 3 types of dental-derived mesenchymal stem cells: dental pulp stem cells, stem cells from human exfoliated deciduous teeth, and stem cells of the apical papilla (SCAP)...
June 5, 2018: Journal of Endodontics
Chiao-Fang Teng, Long-Bin Jeng, Woei-Cherng Shyu
Evidence has emerged that stem cells represent a promising therapeutic tool for tissue engineering and regenerative medicine. Thus, identifying functional markers for selecting stem cells capable of superior self-renewal and pluripotency (or multipotency) and maintaining stem cell identity under appropriate culture conditions are critical for guiding the use of stem cells toward clinical applications. Many investigations have implicated the insulin-like growth factor 1 receptor (IGF1R) signaling in maintenance of stem cell characteristics and enhancement of stem cell therapy efficacy...
January 1, 2018: Cell Transplantation
E E Smith, S Angstadt, N Monteiro, W Zhang, A Khademhosseini, P C Yelick
Tooth loss is a significant health issue currently affecting millions of people worldwide. Artificial dental implants, the current gold standard tooth replacement therapy, do not exhibit many properties of natural teeth and can be associated with complications leading to implant failure. Here we propose bioengineered tooth buds as a superior alternative tooth replacement therapy. We describe improved methods to create highly cellularized bioengineered tooth bud constructs that formed hallmark features that resemble natural tooth buds such as the dental epithelial stem cell niche, enamel knot signaling centers, transient amplifying cells, and mineralized dental tissue formation...
June 1, 2018: Journal of Dental Research
M Strub, L Keller, Y Idoux-Gillet, H Lesot, F Clauss, N Benkirane-Jessel, S Kuchler-Bopp
Transplantation of bone marrow mesenchymal stem cells (BMDCs) into a denervated side of the spinal cord was reported to be a useful option for axonal regeneration. The innervation of teeth is essential for their function and protection but does not occur spontaneously after injury. Cultured reassociations between dissociated embryonic dental mesenchymal and epithelial cells and implantation lead to a vascularized tooth organ regeneration. However, when reassociations were coimplanted with a trigeminal ganglion (TG), innervation did not occur...
June 1, 2018: Journal of Dental Research
W-Z Lew, S-W Feng, C-T Lin, H-M Huang
AIM: To investigate whether static magnetic fields (SMFs) have a positive effect on the migration and dentinogenesis of dental pulp stem cells (DPSCs) to promote reparative dentine formation. METHODOLOGY: In vitro scratch assays and a traumatic pulp exposure model were performed to evaluate the effect of 0.4-Tesla (T) SMF on DPSC migration. The cytoskeletons of the DPSCs were identified by fluorescence immunostaining and compared with those of a sham-exposed group...
June 5, 2018: International Endodontic Journal
Katja Kriebel, Cathleen Hieke, Robby Engelmann, Jan Potempa, Brigitte Müller-Hilke, Hermann Lang, Bernd Kreikemeyer
Periodontitis (PD) is a widespread chronic inflammatory disease in the human population. Porphyromonas gingivalis is associated with PD and can citrullinate host proteins via P. gingivalis peptidyl arginine deiminase (PPAD). Here, we hypothesized that infection of human dental follicle stem cells (hDFSCs) with P. gingivalis and subsequent interaction with neutrophils will alter the neutrophil phenotype. To test this hypothesis, we established and analyzed a triple-culture system of neutrophils and hDFSCs primed with P...
June 1, 2018: Journal of Innate Immunity
Meda-Romana Simu, Emoke Pall, Teodora Radu, Maria Miclaus, Bogdan Culic, Anca-Stefania Mesaros, Alexandrina Muntean, Gabriela Adriana Filip
In this study we designed a composite biomaterial based on a high viscosity soft propolis extract (70% propolis) and shell clam, with antiseptic and osteoinductive qualities, that can be used in dentistry, orthopedics and other areas where hard tissue regeneration is needed. We assessed it in interaction with stabilized human cells isolated from dental papilla of wisdom teeth (D1MSCs). We performed detailed characterization of the obtained material by Scanning Electronic Microscopy (SEM), X-Ray Diffraction (XRD), Energy Dispersive X-Ray Spectroscopy (EDX), Fourier Transform Infrared Spectroscopy (FTIR) techniques...
June 2018: Tissue & Cell
Maziar Ebrahimi Dastgurdi, Fatemeh Ejeian, Marzie Nematollahi, Ahmad Motaghi, Mohammad Hossein Nasr-Esfahani
OBJECTIVE: This study aimed to compare the behavior of dental pulp stem cells (DPSCs) after isolation using solutions containing either collagenase/dispase or collagenase alone. DESIGN: DPSCs were isolated by two digestion methods (collagenase/dispase or collagenase alone) from human third molars. Immunophenotypic features were confirmed by flow cytometry for cell markers STRO-1, cluster of differentiation (CD) 146, CD45, and collagen type-I. The proliferation potential of cells was evaluated by 5-bromo-2'-deoxyuridine (brdU) incorporation assay, and finally they were assessed for multi-lineage differentiation potential...
May 19, 2018: Archives of Oral Biology
Chuan Zhou, An-Tian Xu, Dan-Dan Wang, Guo-Fen Lin, Tie Liu, Fu-Ming He
Recruitment of endogenous bone marrow-derived mesenchymal stem cells (BMSCs) has been widely discussed as an alternative strategy for bone regeneration. Strontium (Sr) is known to direct the BMSCs' commitment to the bone lineage and encourage bone formation; however, the underlying mechanisms remain elusive. In this study, an Sr-incorporated micro/nano rough titanium surface (MNT-Sr) was fabricated by hydrothermal treatment in an attempt to facilitate BMSCs' recruitment and their osteogenic differentiation to enhance rapid osseointegration...
May 31, 2018: Biomaterials Science
Hilary Pada
Dental providers are integral to patients preparing for hematopoietic stem cell transplantation (HSCT) and for survivorship care. Following successful treatment, HSCT patients eventually return to the care of a general dentist, who must understand the importance of assessing and managing these individuals in collaboration with medical colleagues. This case report describes the dental therapy of a patient diagnosed with multiple myeloma who was experiencing teeth chipping in the anterior, clenching, and myofascial tension...
June 2018: Compendium of Continuing Education in Dentistry
Hong Qian, Qun Huang, Yu-Xiang Chen, Qiong Liu, Jing-Xian Fang, Man-Wen Ye
As one type of adult stem cells (ASCs), human dental pulp stem cells (HDPSCs) have several properties, including high proliferation rate, self‑renewal capability, and multi‑lineage differentiation. However, the apoptotic mechanism underlying the development of dental pulp cells remains unclear. In the present study, a significant increase of apoptosis was observed in HDPSCs from the deciduous teeth compared with that from adult permanent teeth. In addition, the occurrence of cytochrome c expression and mitochondrial‑mediated apoptosis pathway activity in HDPSCs were confirmed by quantitative polymerase chain reaction, and western blotting...
May 22, 2018: Molecular Medicine Reports
Mingyue Liu, Lin Zhao, Junlong Hu, Lihua Wang, Ning Li, Di Wu, Xin Shi, Mengtong Yuan, Weiping Hu, Xiaofeng Wang
It has been established that dental pulp stem cells (DPSCs) serve an important role in the restoration and regeneration of dental tissues. DPSCs are present in blood vessels and also exist in the vessel microenvironment in vivo and have a close association with endothelial cells (ECs). The present study aimed to evaluate the influence of ECs and their secretory product endothelin‑1 (ET‑1) on the differentiation of DPSCs. In the present study, cells were divided into four groups: i) a DPSC‑only control group; ii) a DPSC with ET‑1 administration group; iii) a DPSC and human umbilical vein endothelial cell (HUVEC) direct co‑culture group; and iv) a DPSC and HUVEC indirect co‑culture group using a Transwell system...
May 17, 2018: Molecular Medicine Reports
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