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https://www.readbyqxmd.com/read/29353462/lactobacillus-kosoi-sp-nov-a-fructophilic-species-isolated-from-k%C3%A3-so-a-japanese-sugar-vegetable-fermented-beverage
#1
Tai-Ying Chiou, Wataru Suda, Kenshiro Oshima, Masahira Hattori, Chiaki Matsuzaki, Kenji Yamamoto, Tomoya Takahashi
A novel Gram-positive, fructophilic, catalase negative, and rod-shaped strain, designated strain 10HT was isolated from kôso, a Japanese sugar-vegetable fermented beverage obtained from a food processing factory in Saku City, Nagano Prefecture, Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed strain 10HT to belong to the genus Lactobacillus, with closely related type strains being Lactobacillus kunkeei YH-15T (95.5% sequence similarity), Lactobacillus ozensis Mizu2-1T (95.4% sequence similarity), and Lactobacillus apinorum Fhon13NT (95...
January 20, 2018: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29353414/oral-administration-of-compound-probiotics-ameliorates-hfd-induced-gut-microbe-dysbiosis-and-chronic-metabolic-inflammation-via-the-g-protein-coupled-receptor-43-in-non-alcoholic-fatty-liver-disease-rats
#2
Yinji Liang, Shu Liang, Yupei Zhang, Yuanjun Deng, Yifang He, Yanning Chen, Chan Liu, Chenli Lin, Qinhe Yang
The aim of this study was to investigate how the effects of compound probiotics modulate the gut microbiota, short-chain fatty acid (SCFA), body composition, serum and liver lipids, and inflammatory markers in non-alcoholic fatty liver disease (NAFLD) rats. Twenty-four male SD rats were randomly divided into 3 groups: normal control group (standard feed), high-fat diet (HFD) feeding group (83% standard feed + 10% lard oil + 1.5% cholesterol + 0.5% cholate + 5% sucrose), and compound probiotics intervention group (HFD + 0...
January 20, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29353409/16s-rrna-gene-sequencing-reveals-altered-composition-of-gut-microbiota-in-individuals-with-kidney-stones
#3
Ruiqiang Tang, Yonghua Jiang, Aihua Tan, Juan Ye, Xiaoying Xian, Yuanliang Xie, Qiuyan Wang, Ziting Yao, Zengnan Mo
Nephrolithiasis is a common urological disease with high prevalence and recurrence rates. Characterizing gut microbiome profiles of nephrolithiasis patients may provide valuable insights and potential biomarkers for the disease. Therefore, we explored the relation between gut microbiome and nephrolithiasis using 16S ribosomal RNA (rRNA) gene sequencing. 13 patients with multiple kidney stones and 13 matched healthy controls were recruited. A decreasing trend in number of observed species in nephrolithiasis patients was detected, although statistical significance was not reached (p = 0...
January 20, 2018: Urolithiasis
https://www.readbyqxmd.com/read/29353401/temporal-analysis-of-factors-associated-with-eat-10-in-outpatients-with-oropharyngeal-dysphagia-from-a-tertiary-care-clinic
#4
R S Bartlett, J E Moore, S L Thibeault
Self-perception of disease is increasingly recognized as a determinant of health. The Eating Assessment Tool-10 (EAT-10) is a functional health status questionnaire that measures the symptomatic severity of dysphagia from the patient's perspective. The objective of this work was to identify factors (demographics, clinical variables, swallowing physiology, health-related quality of life) associated with longitudinal change in EAT-10 scores in outpatients with oropharyngeal dysphagia at a multi-disciplinary, tertiary care clinic...
January 20, 2018: Dysphagia
https://www.readbyqxmd.com/read/29353271/erythrocytes-and-skeletal-muscle-unsaturated-and-omega-6-fatty-acids-are-positively-correlated-after-caloric-restriction-and-exercise
#5
Miquel Martorell, Victoria Pons, Joan Carles Domingo, Xavier Capó, Antoni Sureda, Franchek Drobnic, Josep-Antoni Tur, Antoni Pons
BACKGROUND: Nutritional intervention studies with fatty acid (FA) supplements assess the efficacy of the intervention by measuring the changes in erythrocyte membrane lipid profiles reflected in tissue composition changes. The aim was to determine the effects of caloric restriction (CR) on erythrocytes lipid composition and to compare and correlate these changes with skeletal muscle acid profiles after CR. METHODS: Erythrocytes were obtained from 11 healthy men before and after 4 weeks of 33% CR in post-exercise conditions; muscle biopsies were obtained from the same athletes after 4 weeks of 33% CR in post-exercise conditions...
January 19, 2018: Annals of Nutrition & Metabolism
https://www.readbyqxmd.com/read/29353265/growth-sexual-and-bone-development-in-a-boy-with-bilateral-anorchia-under-testosterone-treatment-guided-by-the-development-of-his-monozygotic-twin
#6
Sara Vandewalle, Eva Van Caenegem, Margarita Craen, Youri Taes, Jean-Marc Kaufman, Guy T'Sjoen
BACKGROUND: Sex steroids are essential for sexual maturation, linear growth and bone development. However, there is no consensus on the optimal timing, dosage and dosage interval of testosterone therapy to induce pubertal development and achieve a normal adult height and bone mass in children with hypogonadism. CASE PRESENTATION: A monozygotic monochorial male twin pair, of which one boy was diagnosed with anorchia at birth due to testicular regression syndrome was followed from the age of 3 until the age of 18 years...
January 20, 2018: Journal of Pediatric Endocrinology & Metabolism: JPEM
https://www.readbyqxmd.com/read/29353262/preterm-human-milk-macronutrient-concentration-is-independent-of-gestational-age-at-birth
#7
Jan Maly, Iva Burianova, Veronika Vitkova, Eva Ticha, Martina Navratilova, Eva Cermakova
OBJECTIVE: To evaluate the amount of macronutrients in aggregate of human milk samples after preterm delivery during the first 2 months of lactation. METHODS: Analysis of the donated single milk samples, gained by complete emptying of the whole breast at the same daytime between 24+0 and 35+6 gestational age (GA), was designed as prospective observational cohort trial. Two milk samples were analysed every postnatal week up to the discharge from the hospital, week 9 or loss of lactation...
January 20, 2018: Archives of Disease in Childhood. Fetal and Neonatal Edition
https://www.readbyqxmd.com/read/29353192/monte-carlo-calculations-of-the-cellular-s-values-for-%C3%AE-particle-emitting-radionuclides-incorporated-into-the-nuclei-of-cancer-cells-of-the-mda-mb231-mcf7-and-pc3-lines
#8
E L Rojas-Calderón, O Ávila, G Ferro-Flores
S-values (dose per unit of cumulated activity) for alpha particle-emitting radionuclides and monoenergetic alpha sources placed in the nuclei of three cancer cell models (MCF7, MDA-MB231 breast cancer cells and PC3 prostate cancer cells) were obtained by Monte Carlo simulation. The MCNPX code was used to calculate the fraction of energy deposited in the subcellular compartments due to the alpha sources in order to obtain the S-values. A comparison with internationally accepted S-values reported by the MIRD Cellular Committee for alpha sources in three sizes of spherical cells was also performed leading to an agreement within 4% when an alpha extended source uniformly distributed in the nucleus is simulated...
January 10, 2018: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/29353111/investigation-of-phase-separated-polyimide-blend-films-containing-boron-nitride-using-ftir-imaging
#9
Boknam Chae, Deok Gi Hong, Young Mee Jung, Jong Chan Won, Seung Woo Lee
Immiscible aromatic polyimide (PI) blend films and a PI blend film incorporated with thermally conductive boron nitride (BN) were prepared, and their phase separation behaviors were examined by optical microscopy and FTIR imaging. The 2,2'-bis(trifluoromethyl)benzidine (TFMB)-containing and 4,4'-thiodianiline (TDA)-containing aromatic PI blend films and a PI blend/BN composite film show two clearly separated regions; one region is the TFMB-rich phase, and the other region is the TDA-rich phase. The introduction of BN induces morphological changes in the immiscible aromatic PI blend film without altering the composition of either domain...
January 10, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29353105/biomimetic-and-enzyme-responsive-dynamic-hydrogels-for-studying-cell-matrix-interactions-in-pancreatic-ductal-adenocarcinoma
#10
Hung-Yi Liu, Murray Korc, Chien-Chi Lin
The tumor microenvironment (TME) governs all aspects of cancer progression and in vitro 3D cell culture platforms are increasingly developed to emulate the interactions between components of the stromal tissues and cancer cells. However, conventional cell culture platforms are inadequate in recapitulating the TME, which has complex compositions and dynamically changing matrix mechanics. In this study, we developed a dynamic gelatin-hyaluronic acid hybrid hydrogel system through integrating modular thiol-norbornene photopolymerization and enzyme-triggered on-demand matrix stiffening...
January 8, 2018: Biomaterials
https://www.readbyqxmd.com/read/29353072/development-of-subcortical-volumes-across-adolescence-in-males-and-females-a-multisample-study-of-longitudinal-changes
#11
Megan M Herting, Cory Johnson, Kathryn L Mills, Nandita Vijayakumar, Meg Dennison, Chang Liu, Anne-Lise Goddings, Ronald E Dahl, Elizabeth R Sowell, Sarah Whittle, Nicholas B Allen, Christian K Tamnes
The developmental patterns of subcortical brain volumes in males and females observed in previous studies have been inconsistent. To help resolve these discrepancies, we examined developmental trajectories using three independent longitudinal samples of participants in the age-span of 8-22 years (total 216 participants and 467 scans). These datasets, including Pittsburgh (PIT; University of Pittsburgh, USA), NeuroCognitive Development (NCD; University of Oslo, Norway), and Orygen Adolescent Development Study (OADS; The University of Melbourne, Australia), span three countries and were analyzed together and in parallel using mixed-effects modeling with both generalized additive models and general linear models...
January 15, 2018: NeuroImage
https://www.readbyqxmd.com/read/29353015/proteomic-and-functional-variation-within-black-snake-venoms-elapidae-pseudechis
#12
Jonathan Goldenberg, Vittoria Cipriani, Timothy N W Jackson, Kevin Arbuckle, Jordan Debono, Daniel Dashevsky, Nadya Panagides, Maria P Ikonomopoulou, Ivan Koludarov, Bin Li, Renan Castro Santana, Amanda Nouwens, Alun Jones, Chris Hay, Nathan Dunstan, Luke Allen, Brian Bush, John J Miles, Lilin Ge, Hang Fai Kwok, Bryan G Fry
Pseudechis (black snakes) is an Australasian elapid snake genus that inhabits much of mainland Australia, with two representatives confined to Papua New Guinea. The present study is the first to analyse the venom of all 9 described Pseudechis species (plus one undescribed species) to investigate the evolution of venom composition and functional activity. Proteomic results demonstrated that the typical Pseudechis venom profile is dominated by phospholipase A2 toxins. Strong cytotoxicity was the dominant function for most species...
January 15, 2018: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/29353004/leishmania-tropica-isolates-from-non-healed-and-healed-patients-in-iran-a-molecular-typing-and-phylogenetic-analysis
#13
Mehdi Bamorovat, Iraj Sharifi, Mohammad Ali Mohammadi, Sana Eybpoosh, Saeid Nasibi, Mohammad Reza Aflatoonian, Ahmad Khosravi
The precise identification of the parasite species causing leishmaniasis is essential for selecting proper treatment modality. The present study aims to compare the nucleotide variations of the ITS1, 7SL RNA, and Hsp70 sequences between non-healed and healed anthroponotic cutaneous leishmaniasis (ACL) patients in major foci in Iran. A case-control study was carried out from September 2015 to October 2016 in the cities of Kerman and Bam, in the southeast of Iran. Randomly selected skin-scraping lesions of 40 patients (20 non-healed and 20 healed) were examined and the organisms were grown in a culture medium...
January 15, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29352962/improved-systemic-metabolism-and-adipocyte-biology-in-mir-150-knockout-mice
#14
Minsung Kang, Xiaobing Liu, Yuchang Fu, W Timothy Garvey
INTRODUCTION: Short non-coding micro-RNAs (miRNAs) are post-transcriptional factors that directly regulate protein expression by degrading or inhibiting target mRNAs; however, the role of miRNAs in obesity and cardiometabolic disease remains unclarified. Based on our earlier study demonstrating that miR-150 influences lipid metabolism, we have studied effects of miR-150 on systemic metabolism and adipocyte biology. MATERIALS AND METHODS: Metabolic phenotypes including body weight, food intake, body composition, glucose tolerance and insulin sensitivity were assessed in WT and global miR-150 KO male mice fed a high-fat diet...
January 15, 2018: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/29352931/effects-of-chitosan-quaternary-ammonium-salt-on-the-physicochemical-properties-of-sodium-carboxymethyl-cellulose-based-films
#15
Beibei Wang, Xiaodeng Yang, Congde Qiao, Yan Li, Tianduo Li, Chunlin Xu
The effects of N-(2-hydroxyl)-propyl-3-trimethylammonium chitosan chloride (HTCC) on the physicochemical properties of sodium carboxymethyl cellulose (CMC)-based films were investigated. The prepared HTCC/CMC film-forming solutions (FFSs) with varying mass ratios exhibited shear-thinning behavior and typical pseudo-plasticity. The highest apparent viscosity and lowest crossover frequency was obtained for the HTCC/CMC FFS with a mass ratio of 10%, due to the formation of the strongest intermolecular interactions, which also led to the best mechanical properties...
March 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29352926/a-novel-microporous-oxidized-bacterial-cellulose-arginine-composite-and-its-effect-on-behavior-of-fibroblast-endothelial-cell
#16
Hui Qiao, Tengfei Guo, Yudong Zheng, Liang Zhao, Yi Sun, Yang Liu, Yajie Xie
The bacterial cellulose (BC) has been reported widely. Although there are many methods to modify BC, such as the oxidized BC, which is biodegradable and can be used as wound dressing. However, the nanostructure of BC makes it difficult to be oxidized. Importantly, high oxidation degree makes the content of aldehyde high, which make the cell biocompatibility poor. Herein, we fabricated a novel bio-composite based on microporous oxidized BC (MOBC) and in-situ grafted with Arg. The micropores can increase the contact area between BC and oxidizing agent and the reaction between MOBC and Arg, which will enhance the biocompatibility...
March 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29352924/producing-nanofibres-from-carrots-with-a-chemical-free-process
#17
Swambabu Varanasi, Leeav Henzel, Scot Sharman, Warren Batchelor, Gil Garnier
The production of nanofibres (NF) from fresh carrots residue was investigated with a mechanical process without using any pulping or bleaching chemicals. Refining with a PFI mill followed by mechanical fibrillation with a homogenizer was used to produce fine NF. Blanching with hot water was carried out to leach the extractives from carrot fibres prior to refining. The energy required to prepare carrot pulp is one order of magnitude lower than for wood pulp and the fibrillation of nanofibres from carrot residue is four times lower in energy than using wood pulp as feedstock...
March 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29352922/cellulose-nanofiber-embedded-sulfonated-poly-ether-sulfone-membranes-for-proton-exchange-membrane-fuel-cells
#18
Xianlin Xu, Rui Li, Chenxiao Tang, Hang Wang, Xupin Zhuang, Ya Liu, Weimin Kang, Lei Shi
Cellulose nanofibers were embedded into sulfonated poly (ether sulfone) matrix to heighten the water retention and proton conductivity of proton exchange membranes (PEMs). Cellulose nanofibers were obtained by hydrolyzing cellulose acetate nanofibers, which were prepared via electrostatic-induction-assisted solution blow spinning. Morphology, thermal stability, and mechanical properties of the PEMs were investigated. The results showed that proton conductivity, water uptake, and methanol permeability of the composite membranes were improved...
March 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29352919/comprehensive-analysis-of-glycosaminoglycans-from-the-edible-shellfish
#19
Yusuke Okamoto, Kyohei Higashi, Robert J Linhardt, Toshihiko Toida
We have previously reported that the keratan sulfate (KS) disaccharide was branched to the C-3 position of glucuronate in chondroitin sulfate (CS)-E derived from the Mactra chinensis. We carried out the comprehensive disaccharide analysis of GAGs from 10 shellfish, Ruditapes philippinarum, Scapharca broughtonii, Mizuhopecten yessoensis, Turbo cornutus, Crassostrea nippona, Corbicula japonica, Mytilus galloprovincialis, Neptunea intersculpta, Pseudocardium sachalinense and Crassostrea gigas, to better understand the glycan structures in marine organisms...
March 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29352915/silver-i-dithiocarbamate-on-modified-magnetic-cellulose-synthesis-density-functional-theory-study-and-application
#20
Samaneh Sabaqian, Firouzeh Nemati, Hossein Taherpour Nahzomi, Majid M Heravi
A magnetically retrievable catalytic system involving Ag(I) dithiocarbamate supported on functionalized cellulose was prepared. The structure of this hybrid nano-composite was characterized by various techniques. In addition, to describe the metal-ligand interactions present in the nano-composite, covalent and electrostatic interactions, density functional theory model and quantum theory of atoms in molecule method were employed. The resultant nano-composite exhibits remarkable catalytic efficacy in the synthesis of 2-amino-6-(2-oxo-2H-chromen-3-yl)-4-phenylnicotinonitriles from the reaction of 3-acetylcoumarin, aromatic aldehydes, and malononitrile...
March 15, 2018: Carbohydrate Polymers
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