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https://www.readbyqxmd.com/read/29100365/the-value-of-detecting-immunoglobulin-gene-rearrangements-in-the-diagnosis-of-b-cell-lymphoma
#1
Can Lu, QiuYan He, Wei Zhu, ChunYan Fu, JianHua Zhou, YongGuang Tao, Shuang Liu, DeSheng Xiao
Objective: To discuss the clinical value of immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma. Methods: A total of 209 cases of B-cell lymphomas and 35 cases of reactive lymphoid hyperplasia were selected for DNA extraction and PCR amplification using the BIOMED-2 primer system. Gel electrophoresis of heteroduplexes was used to analyze immunoglobulin gene rearrangements. Results: A total of 209 cases of B-cell lymphoma, including 69 extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue, 63 diffuse large B-cell lymphomas, 39 follicular lymphomas, 15 small lymphocytic lymphomas, 6 plasmacytomas, 6 mantle cell lymphomas, 7 nodal marginal zone B-cell lymphomas, and 4 lymphoplasmacytoid lymphomas, were examined...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29084365/views-of-dermatopathologists-about-clonality-assays-in-the-diagnosis-of-cutaneous-t-cell-and-b-cell-lymphoproliferative-disorders
#2
REVIEW
Nneka Comfere, Uma Sundram, M Yadira Hurley, Brian Swick
BACKGROUND: Appropriate use criteria have been developed for many tests using expert judgment, evidence-based practice, and clinical experience. In this context, the opinions of practitioners about clonality assays in various clinical scenarios where cutaneous lymphoma is suspected are reported. METHODS: An Appropriate Use Criteria Task Force sponsored by the American Society of Dermatopathology (ASDP) synthesized clinical scenarios for cutaneous lymphoproliferative disorders (LPD)...
October 30, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/28976766/synthesis-and-assessment-of-peptide-gd-dota-conjugates-targeting-extradomain-b-fibronectin-for-magnetic-resonance-molecular-imaging-of-prostate-cancer
#3
Yajuan Li, Zheng Han, Sarah Roelle, Aidan DeSanto, Rob Sabatelle, Rebecca Schur, Zheng-Rong Lu
Contrast enhanced MRI is commonly used in imaging and treatment planning of prostate cancer. However, no tumor targeting contrast agent is commercially available for accurate detection and characterization of prostate cancer with MRI. Extradomain B fibronectin (EDB-FN), an oncoprotein present in aggressive tumors, is a promising molecular target for detection and stratification of high-risk prostate cancer. In this work, we have identified four small peptides (GVK, IGK, SGV, and ZD2) specific to EDB-FN for tumor targeting...
November 6, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28924718/comprehensive-annotation-and-evolutionary-insights-into-the-canine-canis-lupus-familiaris-antigen-receptor-loci
#4
Jolyon Martin, Hannes Ponstingl, Marie-Paule Lefranc, Joy Archer, David Sargan, Allan Bradley
Dogs are an excellent model for human disease. For example, the treatment of canine lymphoma has been predictive of the human response to that treatment. However, an incomplete picture of canine (Canis lupus familiaris) immunoglobulin (IG) and T cell receptor (TR)-or antigen receptor (AR)-gene loci has restricted their utility. This work advances the annotation of the canine AR loci and looks into breed-specific features of the loci. Bioinformatic analysis of unbiased RNA sequence data was used to complete the annotation of the canine AR genes...
September 19, 2017: Immunogenetics
https://www.readbyqxmd.com/read/28883003/discovery-of-human-similar-gene-fusions-in-canine-cancers
#5
Ronan Ulvé, Mélanie Rault, Mathieu Bahin, Laetitia Lagoutte, Jérôme Abadie, Clotilde De Brito, Jean-Michel Coindre, Nadine Botherel, Audrey Rousseau, Valentin Wucher, Edouard Cadieu, Catherine Thieblemont, Christophe Hitte, Laurence Cornevin, Florian Cabillic, Laura Bachelot, David Gilot, Benoit Hennuy, Thierry Guillaudeux, Arnaud Le Goff, Thomas Derrien, Benoît Hédan, Catherine André
Canine cancers represent a tremendous natural resource due to their incidence and striking similarities to human cancers, sharing similar clinical and pathologic features as well as oncogenic events, including identical somatic mutations. Considering the importance of gene fusions as driver alterations, we explored their relevance in canine cancers. We focused on three distinct human-comparable canine cancers representing different tissues and embryonic origins. Through RNA-Seq, we discovered similar gene fusions as those found in their human counterparts: IGK-CCND3 in B-cell lymphoma, MPB-BRAF in glioma, and COL3A1-PDGFB in dermatofibrosarcoma protuberans-like...
September 7, 2017: Cancer Research
https://www.readbyqxmd.com/read/28860343/antigen-receptor-sequencing-of-paired-bone-marrow-samples-shows-homogeneous-distribution-of-acute-lymphoblastic-leukemia-subclones
#6
Prisca M J Theunissen, David van Zessen, Andrew P Stubbs, Malek Faham, Christian M Zwaan, Jacques J M van Dongen, Vincent H J Van Der Velden
In B-cell precursor acute lymphoblastic leukemia, the initial leukemic cells share the same antigen receptor gene rearrangements. However, due to ongoing rearrangement processes, leukemic cells with different gene rearrangement patterns can develop, resulting in subclone formation. We studied leukemic subclones and their distribution in the bone marrow and peripheral blood at diagnosis. Antigen receptor gene rearrangements (IGH, IGK, TRG, TRD, TRB) were analyzed by next-generation sequencing in seven paired bone marrow samples and five paired bone marrow-peripheral blood samples...
November 2017: Haematologica
https://www.readbyqxmd.com/read/28730642/myc-expression-and-translocation-analyses-in-low-grade-and-transformed-follicular-lymphoma
#7
Sietse M Aukema, Roel van Pel, Inga Nagel, Susanne Bens, Reiner Siebert, Stefano Rosati, Eva van den Berg, Anneke G Bosga-Bouwer, Robby E Kibbelaar, Mels Hoogendoorn, Gustaaf W van Imhoff, Hanneke C Kluin-Nelemans, Philip M Kluin, Marcel Nijland
AIMS: Low-grade follicular lymphoma (FL) (grade 1/2, FL1/2) has an annual risk of transformation of ≈3%, which is associated with aberrations in CDKN2A/B, TP53, and MYC. As in diffuse large B-cell lymphoma, high MYC expression in transformed FL (tFL) might predict a MYC breakpoint. METHODS AND RESULTS: We quantified MYC expression by immunohistochemistry and digital analysis in 41 paired biopsies from 20 patients with FL1/2 with subsequent transformation and in four isolated biopsies of tFL...
July 21, 2017: Histopathology
https://www.readbyqxmd.com/read/28693234/relapsed-anaplastic-lymphoma-kinase-positive-large-b-cell-lymphoma-expressed-cluster-of-differentiation-4-and-cytokeratin-an-initially-misdiagnosed-case-corrected-by-immunoglobulin-%C3%AE%C2%BA-locus-gene-rearrangement-detection
#8
Yunfei Shi, Xianghong Li, Yuqin Song, Lixin Zhou, Qin Feng, Ping Wang, Chen Zhang, Weiping Liu, Yanhua Bai, Yumei Lai
Anaplastic lymphoma kinase (ALK)-positive large B-cell lymphoma (LBCL) is a rare lymphoma subtype. The present study investigated a refractory nodal ALK-positive LBCL case in a 28-year-old Chinese male. It was initially misdiagnosed as ALK-positive anaplastic large cell lymphoma; however, the patient's lesions relapsed and spread widely following a short remission for chemotherapy and the patient succumbed to the disease 3 months' post-autologous stem cell transplantation; thus, a revision was performed. Histologically, the tumor cells exhibited a characteristic immunoblastic morphology with marked cellular pleomorphism...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28662995/simplifying-the-human-serum-proteome-for-discriminating-patients-with-bipolar-disorder-of-other-psychiatry-conditions
#9
Jemmyson Romário de Jesus, Rodrigo Moretto Galazzi, Tatiani Brenelli de Lima, Cláudio Eduardo Muller Banzato, Luiz Fernando de Almeida Lima E Silva, Clarissa de Rosalmeida Dantas, Fábio Cézar Gozzo, Marco Aurélio Zezzi Arruda
PURPOSE: An exploratory analysis using proteomic strategies in blood serum of patients with bipolar disorder (BD), and with other psychiatric conditions such as Schizophrenia (SCZ), can provide a better understanding of this disorder, as well as their discrimination based on their proteomic profile. METHODS: The proteomic profile of blood serum samples obtained from patients with BD using lithium or other drugs (N=14), healthy controls, including non-family (HCNF; N=3) and family (HCF; N=9), patients with schizophrenia (SCZ; N=23), and patients using lithium for other psychiatric conditions (OD; N=4) were compared...
June 27, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28245386/-diagnostic-significance-of-biomed-2-standardized-gene-rearran-gement-system-in-patients-with-non-hodgkin-s-lymphoma
#10
Yan Zhang, Ning Xu, Bao-Jun Dong, Ge Song, Qing-Hua Li, Gang An, Xiao-Fei Ai
OBJECTIVE: To detect the immunoglobulin(Ig) and T cell receptor(TCR) gene rearrangement in bone marrow of non-Hodgkin's lymphoma(NHL) patients by using BIOMED-2 standardized system, and to explore the potential clinical significance of Ig/TCR gene rearrangement. METHODS: DNA was extracted in bone marrow and Formalin-fixed and Paraffin-embedded(FFPE) samples of NHL patients, the Ig/TCR gene rearrangements were analyzed by using BIOMED-2 multiple primers system and multiplex PCR assay...
February 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28191367/composite-diffuse-large-b-cell-and-mantle-cell-lymphoma-a-case-report
#11
Farhan Mohammad, Gwenalyn Garcia, Shiksha Kedia, Juan Ding, Matthew Hurford, Alexander Bershadskiy
Composite lymphoma is an extremely rare clinical entity and is characterized by the presence of two different subtypes of lymphoma in the same lymph node. We report a case of composite lymphoma in a 57-year-old male presenting with leg and groin pain. The right inguinal lymph node biopsy showed large and small cells. Immunohistochemistry was consistent with large cells staining for diffuse large B-cell lymphoma (DLBCL) and small cells positive for mantle cell lymphoma (MCL). Polymerase chain reaction (PCR) analysis of immunoglobulin heavy (IGH) chain and immunoglobulin kappa (IGK) light chain gene rearrangements confirmed that the two were clonally unrelated neoplasms...
January 8, 2017: Curēus
https://www.readbyqxmd.com/read/28099581/minimal-residual-disease-detection-in-tunisian-b-acute-lymphoblastic-leukemia-based-on-immunoglobulin-gene-rearrangements
#12
S Besbes, W S Hamadou, M L Boulland, Y B Youssef, B Achour, H Regaieg, A Khelif, T Fest, Z Soua
IGH gene rearrangement and IGK-Kde gene deletion can be used as molecular markers for the assessment of B lineage acute lymphoblastic leukemia (B-ALL). Minimal residual disease detected based on those markers is currently the most reliable prognosis factor in B-ALL. The aim of this study was to use clonal IGH/IGK-Kde gene rearrangements to confirm B-ALL diagnosis and to evaluate the treatment outcome of Tunisian leukemic patients by monitoring the minimal residual disease (MRD) after induction chemotherapy...
January 16, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/27788226/identification-of-immunoglobulin-gene-sequences-from-a-small-read-number-of-mrna-seq-using-hybridomas
#13
Yuki Kuniyoshi, Kazumitsu Maehara, Takeshi Iwasaki, Masayasu Hayashi, Yuichiro Semba, Masatoshi Fujita, Yuko Sato, Hiroshi Kimura, Akihito Harada, Yasuyuki Ohkawa
Identification of immunoglobulin genes in hybridomas is essential for producing antibodies for research and clinical applications. A couple of methods such as RACE and degenerative PCR have been developed for determination of the Igh and Igl/Igk coding sequences (CDSs) but it has been difficult to process a number of hybridomas both with accuracy and rapidness. Here, we propose a new strategy for antibody sequence determination by mRNA-seq of hybridomas. We demonstrated that hybridomas highly expressed the Igh and Igl/Igk genes and that de novo transcriptome assembly using mRNA-seq data enabled identification of the CDS of both Igh and Igl/Igk accurately...
2016: PloS One
https://www.readbyqxmd.com/read/27614734/combined-ikzf1-and-ig-markers-as-new-tools-for-diagnosis-and-minimal-residual-disease-assessment-in-tunisian-b-all
#14
Sawsen Besbes, Walid-Sabri Hamadou, Marie-Laure Boulland, Marie-Paule Lefranc, Yosra Ben Youssef, Béchir Achour, Abderrahim Khelif, Thierry Fest, Zohra Soua
INTRODUCTION: The monitoring of minimal residual disease (MRD) approach in patients diagnosed with B-acute lymphoblastic leukemia (B-ALL) allows an early detection of residual clones inducing relapses and therefore appropriate therapy strategy. The molecular markers may identify and quantify the residual blasts in B-ALL with normal cytology. In this study, we aimed to use combined IKZF1, IGH and IGK immunoglobulin genes for diagnosis and MRD monitoring in B-ALL sample using MLPA, multiplex PCR and real-time quantitative PCR...
October 2016: Bulletin du Cancer
https://www.readbyqxmd.com/read/27439714/identification-of-multi-loci-hubs-from-4c-seq-demonstrates-the-functional-importance-of-simultaneous-interactions
#15
Tingting Jiang, Ramya Raviram, Valentina Snetkova, Pedro P Rocha, Charlotte Proudhon, Sana Badri, Richard Bonneau, Jane A Skok, Yuval Kluger
Use of low resolution single cell DNA FISH and population based high resolution chromosome conformation capture techniques have highlighted the importance of pairwise chromatin interactions in gene regulation. However, it is unlikely that associations involving regulatory elements act in isolation of other interacting partners that also influence their impact. Indeed, the influence of multi-loci interactions remains something of an enigma as beyond low-resolution DNA FISH we do not have the appropriate tools to analyze these...
October 14, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27301320/collagen-epitope-expression-on-b-cells-is-sufficient-to-confer-tolerance-to-collagen-induced-arthritis
#16
Sofia E M Andersson, Tove Eneljung, Sara Tengvall, Pernilla Jirholt, Anna Stern, Louise Henningsson, Bibo Liang, Katrin Thorarinsdottir, Jan Kihlberg, Rikard Holmdahl, Inga-Lill Mårtensson, Kenth Gustafsson, Inger Gjertsson
BACKGROUND: The mechanisms underlying tolerance induction and maintenance in autoimmune arthritis remain elusive. In a mouse model of rheumatoid arthritis, collagen type II (CII)-induced arthritis, we explore the contribution of B cells to antigen-specific tolerance. METHODS: To generate expression of the CII-peptide specifically on B-cell major histocompatibility complex type II, lentiviral-based gene therapy including a B-cell-specific Igk promoter was used. RESULTS: Presentation of the CII-peptide on B cells significantly reduced the frequency and severity of arthritis as well as the serum levels of CII -specific IgG antibodies...
June 14, 2016: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/27261530/molecular-functions-of-the-transcription-factors-e2a-and-e2-2-in-controlling-germinal-center-b-cell-and-plasma-cell-development
#17
Miriam Wöhner, Hiromi Tagoh, Ivan Bilic, Markus Jaritz, Daniela Kostanova Poliakova, Maria Fischer, Meinrad Busslinger
E2A is an essential regulator of early B cell development. Here, we have demonstrated that E2A together with E2-2 controlled germinal center (GC) B cell and plasma cell development. As shown by the identification of regulated E2A,E2-2 target genes in activated B cells, these E-proteins directly activated genes with important functions in GC B cells and plasma cells by inducing and maintaining DNase I hypersensitive sites. Through binding to multiple enhancers in the Igh 3' regulatory region and Aicda locus, E-proteins regulated class switch recombination by inducing both Igh germline transcription and AID expression...
June 27, 2016: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/27239026/active-and-inactive-enhancers-cooperate-to-exert-localized-and-long-range-control-of-gene-regulation
#18
Charlotte Proudhon, Valentina Snetkova, Ramya Raviram, Camille Lobry, Sana Badri, Tingting Jiang, Bingtao Hao, Thomas Trimarchi, Yuval Kluger, Iannis Aifantis, Richard Bonneau, Jane A Skok
V(D)J recombination relies on the presence of proximal enhancers that activate the antigen receptor (AgR) loci in a lineage- and stage-specific manner. Unexpectedly, we find that both active and inactive AgR enhancers cooperate to disseminate their effects in a localized and long-range manner. Here, we demonstrate the importance of short-range contacts between active enhancers that constitute an Igk super-enhancer in B cells. Deletion of one element reduces the interaction frequency between other enhancers in the hub, which compromises the transcriptional output of each component...
June 7, 2016: Cell Reports
https://www.readbyqxmd.com/read/27212160/ascites-as-the-initial-characteristic-manifestation-in-a-patient-with-primary-gastric-cd8-positive-diffuse-large-b-cell-lymphoma
#19
K-X Zhao, G-Z Dai, J-F Zhu
CASE PRESENTATION: Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoid malignancy and the most common type of non-Hodgkin's lymphomas, the stomach is the most common extranodal site. Gastric DLBCL is often characterized by epigastric pain and vomiting. We report a case of a 78-year-old female patient with gastric diffuse large B-cell lymphoma (DLBCL) with high CD8 level which was initially manifested with ascites of unknown origin. The patient was admitted with a chief complaint of abdominal distension and scanty urine over the last twenty days, while without anorexia and fatigue until 15 March...
May 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27169754/molecular-clonality-assessment-shows-high-performance-to-predict-malignant-b-cell-non-hodgkin-s-lymphoma-using-cytological-smears
#20
Paul Roepman, Cecile-Marie Boots, Karen C Scheidel, Tobias Sprong, Peter de Bruin, Okke de Weerdt, Patricia J T A Groenen, J Alain Kummer
AIMS: Molecular PCR-based clonality analysis is helpful for identification of monoclonal B-cell or T-cell populations and to distinguish malignant lymphoma from reactive lymphoid hyperplasia. Typically, clonality assessment on fine-needle aspiration cytology (FNAC) requires freshly obtained aspirates, but the collection and processing of these samples are often challenging in daily practice. In this study, we assessed the routine diagnostic value of the EuroClonality/BIOMED-2 assay for B-cell clonality on air-dried archived Giemsa-stained smears...
December 2016: Journal of Clinical Pathology
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