Luisa Lorenzi, Silvia Lonardi, Michela Bonezzi, Stefania Zini, Mattia Bugatti, Arianna Valzelli, Flavia Melotti, Mattia Facchetti, Iacopo Ghini, Vincenzo Villanacci, Piera Balzarini, Marco Pizzi, Viviana Giustini, Anna Galvagni, Marco Chiarini, Angelo Paolo Dei Tos, William Vermi, Stefano Casola, Fabio Facchetti
Evaluation of B-cell clonality can be challenging in the interpretation of lymphoid infiltrates on tissue sections. Clonality testing based on IG gene rearrangements analysis by PCR (IG-PCR) is the gold standard. Alternatively, B-cell clonality can be assessed by the recognition of immunoglobulin light chain (IgLC) restriction, by immunohistochemistry (IHC), chromogenic in situ hybridization (ISH) or flow cytometry (FC). IG-PCR requires molecular facilities, and FC requires cell suspensions, both not widely available in routine pathology units...
October 26, 2023: Virchows Archiv: An International Journal of Pathology