keyword
https://read.qxmd.com/read/36897397/a-comparison-of-special-intrauterine-balloons-and-intrauterine-contraceptive-devices-in-the-treatment-of-intrauterine-adhesions
#21
JOURNAL ARTICLE
Lin-Lin Zhuang, Kai Wang, Hai-Lan Shen, Jia-Hui Lin, Ye Lu, Zhen-Yu Luo, Wen-Rong Wang
PURPOSE: This study aimed to compare the efficacy of a special kind of intrauterine balloon (IUB) and that of an intrauterine contraception device (IUD) for patients with intrauterine adhesions (IUAs) after transcervical resection of adhesion (TCRA). METHODS: In this retrospective cohort study, after TCRA, 31 patients received a special IUB, and 38 patients received an IUD. The Fisher exact test, logistic regression method, Kaplan-Meier method and Cox proportional hazards regression model were used for statistical analysis...
March 10, 2023: Archives of Gynecology and Obstetrics
https://read.qxmd.com/read/36574344/analysis-of-t-cell-repertoire-and-transcriptome-identifies-mechanisms-of-regulatory-t-cell-treg-suppression-of-gvhd
#22
JOURNAL ARTICLE
Juliane K Lohmeyer, Toshihito Hirai, Mustafa Turkoz, Stephane Buhler, Teresa Lopes Ramos, Natalie Köhler, Jeanette Baker, Astrid Melotti, Ingrid Wagner, Amandine Pradier, Sisi Wang, Xuhuai Ji, Simone Becattini, Jean Villard, Doron Merkler, Yves Chalandon, Robert S Negrin, Federico Simonetta
CD4+FOXP3+ regulatory T cells have demonstrated efficacy in graft-versus-host disease (GvHD) prevention and treatment. Preclinical and clinical studies indicate that Treg are able to protect from GvHD without interfering with the graft-versus-tumor (GvT) effect of hematopoietic cell transplantation (HCT), although the underlying molecular mechanisms are largely unknown. To elucidate Treg suppressive function during in vivo suppression of acute GvHD, we performed paired T cell receptor (TCRa, TCRb genes) repertoire sequencing and RNA sequencing analysis on conventional T cells (Tcon) and Treg before and after transplantation in an MHC major-mismatch mouse model of HCT...
December 27, 2022: Blood
https://read.qxmd.com/read/36482732/the-effect-of-the-combined-use-of-an-intrauterine-device-and-a-foley-balloon-in-the-prevention-of-adhesion-following-hysteroscopic-adhesiolysis
#23
JOURNAL ARTICLE
Jun Zhao, Lei Zhang, Jun Liang, Huan Zhao, Ziwen Wang, Yicun Pang
The aim of this study was to determine whether intrauterine device (IUD) combined with Foley balloon could obtain better efficacy in preventing re-adhesion for patients with intrauterine adhesions (IUAs). The data of 89 patients with IUAs, who underwent transcervical resection of adhesion (TCRA) operation, were retrospectively collected. According to the method used for preventing re-adhesion of the uterine cavity after TCRA, the enrolled patients were divided into IUD group, Foley balloon group and the combined group...
January 2023: Women & Health
https://read.qxmd.com/read/36374463/methods-for-study-of-mouse-t-cell-receptor-%C3%AE-and-%C3%AE-gene-rearrangements
#24
JOURNAL ARTICLE
Danielle J Dauphars, Glendon Wu, Craig H Bassing, Michael S Krangel
Quantitative real-time PCR and next-generation sequencing (NGS) are invaluable techniques to analyze T cell receptor (Tcr) gene rearrangements in mouse lymphocyte populations. Although these approaches are powerful, they also have limitations that must be accounted for in experimental design and data interpretation. Here, we provide relevant background required for understanding these limitations and then outline established quantitative real-time PCR and NGS methods that can be used for analysis of mouse Tcra and Tcrb gene rearrangements in mice...
2023: Methods in Molecular Biology
https://read.qxmd.com/read/36138028/chromatin-organizer-satb1-controls-the-cell-identity-of-cd4-cd8-double-positive-thymocytes-by-regulating-the-activity-of-super-enhancers
#25
JOURNAL ARTICLE
Delong Feng, Yanhong Chen, Ranran Dai, Shasha Bian, Wei Xue, Yongchang Zhu, Zhaoqiang Li, Yiting Yang, Yan Zhang, Jiarui Zhang, Jie Bai, Litao Qin, Yoshinori Kohwi, Weili Shi, Terumi Kohwi-Shigematsu, Jing Ma, Shixiu Liao, Bingtao Hao
CD4+ and CD8+ double-positive (DP) thymocytes play a crucial role in T cell development in the thymus. DP cells rearrange the T cell receptor gene Tcra to generate T cell receptors with TCRβ. DP cells differentiate into CD4 or CD8 single-positive (SP) thymocytes, regulatory T cells, or invariant nature kill T cells (iNKT) in response to TCR signaling. Chromatin organizer SATB1 is highly expressed in DP cells and is essential in regulating Tcra rearrangement and differentiation of DP cells. Here we explored the mechanism of SATB1 orchestrating gene expression in DP cells...
September 22, 2022: Nature Communications
https://read.qxmd.com/read/36049892/chronic-endometritis-positively-correlates-with-the-aggravation-of-intrauterine-adhesions-but-has-limited-effects-on-reproductive-prognosis-with-antibiotic-application
#26
JOURNAL ARTICLE
Rui Xiang, Manchao Li, Zhongjia Gu, Huijun Liu, Haitao Zeng, Jintao Peng
OBJECTIVE: To assess the effect of chronic endometritis (CE) diagnosed by CD138 staining on the aggravation of intrauterine adhesions (IUAs), and the reproductive prognosis after transcervical resection of adhesions (TCRA). METHODS: Sixty-three patients with severe IUAs (group A) and 119 patients with moderate IUAs (group B) were included in this retrospective study. TCRA and endometrial biopsy with CD138 staining were performed. Participants in each group were classified into two subgroups: CE group and NCE group (without CE)...
September 1, 2022: International Journal of Gynaecology and Obstetrics
https://read.qxmd.com/read/36038909/estradiol-and-intrauterine-device-treatment-for-moderate-and-severe-intrauterine-adhesions-after-transcervical-resection
#27
JOURNAL ARTICLE
Yun Sun, Xiuying Chen, Zhida Qian, Lili Cao, Sifeng Zhan, Lili Huang
OBJECTIVE: To explore the effect of 4 mg/day, 6 mg/day, and 8 mg/day estradiol alone or in combination with an intrauterine device (IUD) in patients with moderate and severe intrauterine adhesion (IUA) after transcervical resection of adhesion (TCRA). METHODS: Patients with moderate or severe IUA who reived 4 mg/day, 6 mg/day, and 8 mg/day estradiol alone or in combination with an intrauterine device (IUD) after TCRA in Women's Hospital, Zhejiang University School of Medicine, from March 2014 to December 2014 were enrolled in this retrospective case-control study...
August 29, 2022: BMC Women's Health
https://read.qxmd.com/read/36005811/involvement-of-bldc-in-the-formation-of-physiologically-mature-sporangium-in-actinoplanes-missouriensis
#28
JOURNAL ARTICLE
Takeaki Tezuka, Shumpei Nitta, Yasuo Ohnishi
AmBldD is a global transcriptional regulator that represses the transcription of several genes required for sporangium formation in Actinoplanes missouriensis. Here, we characterized one of the AmBldD regulons: AMIS_1980 , encoding an ortholog of BldC, which is a transcriptional regulator involved in the morphological development of Streptomyces. We determined the transcriptional start point of the bldC ortholog by high-resolution S1 nuclease mapping and found an AmBldD box in its 5'-untranslated region. Reverse transcription-quantitative PCR analysis revealed that the transcription of bldC is activated during sporangium formation...
August 25, 2022: Journal of Bacteriology
https://read.qxmd.com/read/35921443/tcf-1-promotes-genomic-instability-and-t-cell-transformation-in-response-to-aberrant-%C3%AE-catenin-activation
#29
JOURNAL ARTICLE
Stephen Arnovitz, Priya Mathur, Melissa Tracy, Azam Mohsin, Soumi Mondal, Jasmin Quandt, Kyle M Hernandez, Khashayarsha Khazaie, Marei Dose, Akinola Olumide Emmanuel, Fotini Gounari
Understanding the mechanisms promoting chromosomal translocations of the rearranging receptor loci in leukemia and lymphoma remains incomplete. Here we show that leukemias induced by aberrant activation of β-catenin in thymocytes, which bear recurrent Tcra/Myc-Pvt1 translocations, depend on Tcf-1. The DNA double strand breaks (DSBs) in the Tcra site of the translocation are Rag-generated, whereas the Myc-Pvt1 DSBs are not. Aberrantly activated β-catenin redirects Tcf-1 binding to novel DNA sites to alter chromatin accessibility and down-regulate genome-stability pathways...
August 9, 2022: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/35804057/donor-genetic-determinant-of-thymopoiesis-rs2204985-impacts-clinical-outcome-after-single-hla-mismatched-hematopoietic-stem-cell-transplantation
#30
JOURNAL ARTICLE
Chrysanthi Tsamadou, Sowmya Gowdavally, Uwe Platzbecker, Elisa Sala, Thomas Valerius, Eva Wagner-Drouet, Gerald Wulf, Nicolaus Kröger, Niels Murawski, Hermann Einsele, Kerstin Schaefer-Eckart, Sebastian Freitag, Jochen Casper, Martin Kaufmann, Mareike Dürholt, Bernd Hertenstein, Stefan Klein, Mark Ringhoffer, Sandra Frank, Christine Neuchel, Immanuel Rode, Hubert Schrezenmeier, Joannis Mytilineos, Daniel Fuerst
A common genetic variant within the T cell receptor alpha (TCRA)-T cell receptor delta (TCRD) locus (rs2204985) has been recently found to associate with thymic function. Aim of this study was to investigate the potential impact of donor rs2204985 genotype on patient's outcome after unrelated hematopoietic stem cell transplantation (uHSCT). 2016 adult patients were retrospectively analyzed. rs2204985 genotyping was performed by next generation sequencing, p < 0.05 was considered significant and donor rs2204985 GG/AG genotypes were set as reference vs...
July 8, 2022: Bone Marrow Transplantation
https://read.qxmd.com/read/35557274/deletion-of-myd88-in-t-cells-worsens-cardiac-pathology-through-enhanced-t-cell-survival-and-induction-of-cardiac-fibrosis
#31
JOURNAL ARTICLE
Abraham L Bayer, Sandra Smolgovsky, Njabulo Ngwenyama, Mark Aronovitz, Kuljeet Kaur, Pilar Alcaide
Heart failure (HF) is a leading cause of death worldwide, associated with cardiac and systemic inflammation. However, no anti-inflammatory therapies have shown success. Damage associated molecular patterns (DAMPs) released in the heart activate myeloid cells through the adaptor "Myeloid differentiation primary response 88" (MyD88). This signaling cascade promotes cardiac antigen presentation to activate helper T-cells. Once activated, T-cells then infiltrate the heart and bind to VCAM-1 on cardiac fibroblasts, (CFB), transforming them to myofibroblasts which coordinate cardiac fibrosis...
May 2022: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/35464421/sleeping-with-the-enemy-the-current-knowledge-of-piscine-orthoreovirus-prv-immune-response-elicited-to-counteract-infection
#32
REVIEW
Eva Vallejos-Vidal, Felipe E Reyes-López, Ana María Sandino, Mónica Imarai
Piscine orthoreovirus (PRV) is a virus in the genus Orthoreovirus of the Reoviridae family, first described in 2010 associated with Heart and Skeletal Muscle Inflammation (HSMI) in Atlantic salmon ( Salmo salar ). Three phases of PRV infection have been described, the early entry and dissemination, the acute dissemination phase, and the persistence phase. Depending on the PRV genotype and the host, infection can last for life. Mechanisms of immune response to PRV infection have been just beginning to be studied and the knowledge in this matter is here revised...
2022: Frontiers in Immunology
https://read.qxmd.com/read/35449839/observation-of-the-auxiliary-treatment-effect-of-low-frequency-nerve-therapy-instrument-after-hysteroscopy-for-moderate-and-severe-intrauterine-adhesions-based-on-intelligent-medical-treatment
#33
JOURNAL ARTICLE
Mingyue Yin, Yingzheng Pan
Uterine adhesions are mainly manifested as menstrual changes in women of childbearing age and affect fertility. Resection of uterine adhesions can well restore the shape of the patient's uterine cavity and improve the patient's menstruation. However, how to promote the growth of the endometrium and prevent the recurrence of adhesions after the operation is still a major problem. This article aims to study the use of traditional treatment methods as a control and use low-frequency nerve therapy device to assist in the treatment of posterior intrauterine adhesions recurrence rate, menstrual recovery effective rate, adverse reaction rate, liver function, etc...
2022: Journal of Healthcare Engineering
https://read.qxmd.com/read/35411048/single-cell-rna-sequencing-coupled-to-tcr-profiling-of-large-granular-lymphocyte-leukemia-t-cells
#34
JOURNAL ARTICLE
Shouguo Gao, Zhijie Wu, Bradley Arnold, Carrie Diamond, Sai Batchu, Valentina Giudice, Lemlem Alemu, Diego Quinones Raffo, Xingmin Feng, Sachiko Kajigaya, John Barrett, Sawa Ito, Neal S Young
T-cell large granular lymphocyte leukemia (T-LGLL) is a lymphoproliferative disease and bone marrow failure syndrome which responds to immunosuppressive therapies. We show single-cell TCR coupled with RNA sequencing of CD3+ T cells from 13 patients, sampled before and after alemtuzumab treatments. Effector memory T cells and loss of T cell receptor (TCR) repertoire diversity are prevalent in T-LGLL. Shared TCRA and TCRB clonotypes are absent. Deregulation of cell survival and apoptosis gene programs, and marked downregulation of apoptosis genes in CD8+ clones, are prominent features of T-LGLL cells...
April 11, 2022: Nature Communications
https://read.qxmd.com/read/34970966/robust-t-cell-activation-requires-an-eif3-driven-burst-in-t-cell-receptor-translation
#35
JOURNAL ARTICLE
Dasmanthie DeSilva, Lucas Ferguson, Grant H Chin, Benjamin E Smith, Ryan A Apathy, Theodore L Roth, Franziska Blaeschke, Marek Kudla, Alexander Marson, Nicholas T Ingolia, Jamie Hd Cate
Activation of T cells requires a rapid surge in cellular protein synthesis. However, the role of translation initiation in the early induction of specific genes remains unclear. Here we show human translation initiation factor eIF3 interacts with select immune system related mRNAs including those encoding the T cell receptor (TCR) subunits TCRA and TCRB. Binding of eIF3 to the TCRA and TCRB mRNA 3'-untranslated regions (3'-UTRs) depends on CD28 coreceptor signaling and regulates a burst in TCR translation required for robust T cell activation...
December 31, 2021: ELife
https://read.qxmd.com/read/34910107/trav15-dv6-family-tcrd-rearrangements-diversify-the-tcra-repertoire
#36
JOURNAL ARTICLE
Danielle J Dauphars, Ariana Mihai, Liuyang Wang, Yuan Zhuang, Michael S Krangel
The Tcra repertoire is generated by multiple rounds of Vα-Jα rearrangement. However, Tcrd recombination precedes Tcra recombination within the complex Tcra-Tcrd locus. Here, by ablating Tcrd recombination, we report that Tcrd rearrangement broadens primary Vα use to diversify the Tcra repertoire in mice. We reveal that use of Trav15-dv6 family V gene segments in Tcrd recombination imparts diversity in the Tcra repertoire by instigating use of central and distal Vα segments. Moreover, disruption of the regions containing these genes and their cis-regulatory elements identifies the Trav15-dv6 family as being responsible for driving central and distal Vα recombinations beyond their roles as substrates for Tcrd recombination...
February 7, 2022: Journal of Experimental Medicine
https://read.qxmd.com/read/34819167/recent-trends-in-therapeutic-strategies-for-repairing-endometrial-tissue-in-intrauterine-adhesion
#37
REVIEW
Junyan Ma, Hong Zhan, Wen Li, Liqi Zhang, Feng Yun, Ruijin Wu, Jun Lin, Yangyang Li
Intrauterine adhesion (IUA) is a common gynaecological disease that develops from infection or trauma. IUA disease may seriously affect the physical and mental health of women of childbearing age, which may lead to symptoms such as hypomenorrhea or infertility. Presently, hysteroscopic transcervical resection of adhesion (TCRA) is the principal therapy for IUAs, although its function in preventing the recurrence of adhesion and preserving fertility is limited. Pharmaceuticals such as hormones and vasoactive agents and the placement of nondegradable stents are the most common postoperative adjuvant therapy methods...
November 24, 2021: Biomaterials Research
https://read.qxmd.com/read/34621732/application-of-bioactive-hydrogels-for-functional-treatment-of-intrauterine-adhesion
#38
REVIEW
Jingying Wang, Chao Yang, Yuxin Xie, Xiaoxu Chen, Ting Jiang, Jing Tian, Sihui Hu, Yingli Lu
Intrauterine adhesion (IUA) is a common endometrial disease and one of the main causes of infertility in women of childbearing age. Current treatment strategies, such as hysteroscopic adhesion resection, hysteroscopic transcervical resection of adhesion (TCRA), the use of local hormone drugs, and anti-adhesion scaffold implantation, do not provide a satisfactory pregnancy outcome for moderate-severe IUA, which presents a great challenge in reproductive medicine. With the development of material engineering, various bioactive and functional hydrogels have been developed using natural and synthetic biomaterials...
2021: Frontiers in Bioengineering and Biotechnology
https://read.qxmd.com/read/34601835/post-hysteroscopy-duration-of-the-cook-balloon-uterine-stent-effect-on-the-re-adhesion-formation
#39
RANDOMIZED CONTROLLED TRIAL
Shuyun Qu, Pei Zhou
OBJECTIVE: To compare duration of Cook balloon uterine stent on re-adhesions formation, and observe its effect on serum transforming growth factor β1 (TGF-β1) and plasminogen activator inhibitor-1 (PAI-1) levels in patients with intrauterine adhesions (IUA) undergoing hysteroscopic transcervical resection of adhesion (TCRA). STUDY DESIGN: Randomised controlled trial. PLACE AND DURATION OF STUDY: No. 215 Hospital of Shaanxi Nuclear Industry, Shaanxi Province, China, from January 2018 to January 2019...
October 2021: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://read.qxmd.com/read/34497419/using-dna-sequencing-data-to-quantify-t-cell-fraction-and-therapy-response
#40
JOURNAL ARTICLE
Robert Bentham, Kevin Litchfield, Thomas B K Watkins, Emilia L Lim, Rachel Rosenthal, Carlos Martínez-Ruiz, Crispin T Hiley, Maise Al Bakir, Roberto Salgado, David A Moore, Mariam Jamal-Hanjani, Charles Swanton, Nicholas McGranahan
The immune microenvironment influences tumour evolution and can be both prognostic and predict response to immunotherapy1,2 . However, measurements of tumour infiltrating lymphocytes (TILs) are limited by a shortage of appropriate data. Whole-exome sequencing (WES) of DNA is frequently performed to calculate tumour mutational burden and identify actionable mutations. Here we develop T cell exome TREC tool (T cell ExTRECT), a method for estimation of T cell fraction from WES samples using a signal from T cell receptor excision circle (TREC) loss during V(D)J recombination of the T cell receptor-α gene (TCRA (also known as TRA))...
September 2021: Nature
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