Joel Gibson, Shadi Shokralla, Teresita M Porter, Ian King, Steven van Konynenburg, Daniel H Janzen, Winnie Hallwachs, Mehrdad Hajibabaei
Conventional assessments of ecosystem sample composition are based on morphology-based or DNA barcode identification of individuals. Both approaches are costly and time-consuming, especially when applied to the large number of specimens and taxa commonly included in ecological investigations. Next-generation sequencing approaches can overcome the bottleneck of individual specimen isolation and identification by simultaneously sequencing specimens of all taxa in a bulk mixture. Here we apply multiple parallel amplification primers, multiple DNA barcode markers, 454-pyrosequencing, and Illumina MiSeq sequencing to the same sample to maximize recovery of the arthropod macrobiome and the bacterial and other microbial microbiome of a bulk arthropod sample...
June 3, 2014: Proceedings of the National Academy of Sciences of the United States of America