keyword
MENU ▼
Read by QxMD icon Read
search

bioinformatic cancer

keyword
https://www.readbyqxmd.com/read/28091987/expression-and-prognostic-value-of-micrornas-in-lower-grade-glioma-depends-on-idh1-2-status
#1
Wen Cheng, Xiufang Ren, Chuanbao Zhang, Sheng Han, Anhua Wu
Histological and genomic characteristics are widely used in glioma management and research. This study investigated their relationship to the expression and prognostic value of microRNAs (miRNAs) in lower-grade glioma (LGG). A total of 447 LGG samples with available clinical and genomic information from The Cancer Genome Atlas database were reviewed. Samples with isocitrate dehydrogenase (IDH) 1/2 mutations (n = 366) were randomly divided into training and validation sets to establish and confirm a four-miRNA-based risk classifier...
January 16, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28088504/novel-insights-into-the-transport-mechanism-of-the-human-amino-acid-transporter-lat1-slc7a5-probing-critical-residues-for-substrate-translocation
#2
Lara Napolitano, Michele Galluccio, Mariafrancesca Scalise, Chiara Parravicini, Luca Palazzolo, Ivano Eberini, Cesare Indiveri
BACKGROUND: LAT1 (SLC7A5) is the transport competent unit of the heterodimer formed with the glycoprotein CD98 (SLC3A2). It catalyzes antiport of His and some neutral amino acids such as Ile, Leu, Val, Cys, Met, Gln and Phe thus being involved in amino acid metabolism. Interestingly, LAT1 is over-expressed in many human cancers that are characterized by increased demand of amino acids. Therefore LAT1 was recently acknowledged as a novel target for cancer therapy. However, knowledge on molecular mechanism of LAT1 transport is still scarce...
January 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28086946/mir-1307-promotes-ovarian-cancer-cell-chemoresistance-by-targeting-the-ing5-expression
#3
Wen-Ting Chen, Yu-Jia Yang, Zhen-Dong Zhang, Qiang An, Na Li, Wei Liu, Bing Yang
BACKGROUND: We aimed to investigate the function of miR-1307 in chemoresistance and to explore its chemoresistance mechanism in ovarian cancer. METHODS: IC50 determination was used to test the chemoresistance profling in ovarian cancer cells. QRT-PCR or western blot was used to validate the expression level of miR-1307 and candidate gene or protein. Colony formation assay and FITC-labeled enhanced Annexin V immunofluorescence were used to compare cell proliferation and apoptosis ability, respectively...
January 11, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28081246/nitrosothiol-trapping-based-proteomic-analysis-of-s-nitrosylation-in-human-lung-carcinoma-cells
#4
Shani Ben-Lulu, Tamar Ziv, Pnina Weisman-Shomer, Moran Benhar
Nitrosylation of cysteines residues (S-nitrosylation) mediates many of the cellular effects of nitric oxide in normal and diseased cells. Recent research indicates that S-nitrosylation of certain proteins could play a role in tumor progression and responsiveness to therapy. However, the protein targets of S-nitrosylation in cancer cells remain largely unidentified. In this study, we used our recently developed nitrosothiol trapping approach to explore the nitrosoproteome of human A549 lung carcinoma cells treated with S-nitrosocysteine or pro-inflammatory cytokines...
2017: PloS One
https://www.readbyqxmd.com/read/28081052/identification-of-key-transcription-factors-associated-with-lung-squamous-cell-carcinoma
#5
Feng Zhang, Xia Chen, Ke Wei, Daoming Liu, Xiaodong Xu, Xing Zhang, Hong Shi
BACKGROUND Lung squamous cell carcinoma (lung SCC) is a common type of lung cancer, but its mechanism of pathogenesis is unclear. The aim of this study was to identify key transcription factors in lung SCC and elucidate its mechanism. MATERIAL AND METHODS Six published microarray datasets of lung SCC were downloaded from Gene Expression Omnibus (GEO) for integrated bioinformatics analysis. Significance analysis of microarrays was used to identify differentially expressed genes (DEGs) between lung SCC and normal controls...
January 12, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28076325/identification-of-long-noncoding-rnas-for-the-detection-of-early-stage-lung-squamous-cell-carcinoma-by-microarray-analysis
#6
Zule Cheng, Yanan Bai, Ping Wang, Zhenhua Wu, Lin Zhou, Ming Zhong, Qinghui Jin, Jianlong Zhao, Hailei Mao, Hongju Mao
The aberrant expressions of long noncoding RNAs have been reported in numerous cancers, which have facilitated the cancer diagnosis. However, the expression profile of lncRNAs in early stage lung squamous cell carcinoma has not been well discussed. The present study aimed to examine the expression profile of lncRNAs in early stage lung squamous cell carcinoma and identify lncRNA biomarkers for diagnosis. Through high-throughput lncRNA microarray, we screened thousands of aberrantly expressed lncRNAs and mRNAs in early stage lung squamous cell carcinoma tissues compared to their corresponding adjacent nontumorous tissues...
January 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28075453/mir-375-inhibits-cancer-stem-cell-phenotype-and-tamoxifen-resistance-by-degrading-hoxb3-in-human-er-positive-breast-cancer
#7
Hui Fu, Lei Fu, Chao Xie, Wen-Shu Zuo, Yan-Song Liu, Mei-Zhu Zheng, Jin-Ming Yu
Cancer stem cell (CSC) formation and epithelial-mesenchymal transition (EMT) are pivotal events in tumor cell invasion and metastasis. They have been shown to occur in resistance to tamoxifen. Moreover, microRNAs (miRNAs) have been associated with CSCs, EMT as well as tamoxifen resistance. Studying molecular mechanism of CSCs, EMT as well as tamoxifen resistance will help us to further understand the pathogenesis and progression of the disease and offer new targets for effective therapies. In the present study, we showed that miR-375 inhibits CSC traits in breast cancer MCF-7 cells...
January 9, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28075452/a-novel-pathway-in-nsclc-cells-mir%C3%A2-191-targeting-nfia-is-induced-by-chronic-hypoxia-and-promotes-cell-proliferation-and-migration
#8
Jia Zhao, Cheng-Rui Qiao, Zheng Ding, Yin-Liang Sheng, Xiang-Nan Li, Yang Yang, Deng-Yan Zhu, Chun-Yang Zhang, Dong-Lei Liu, Kai Wu, Song Zhao
MicroRNAs (miRs) have emerged as being important in cancer biology. miR‑191 is a conserved miRNA, which has been investigated in detail and is reported to be induced by hypoxia-inducible factor (HIF)‑1α and has an contributory action in the progression of breast, hepatic and pancreatic cancer. However, the effects of miR‑191 in the progression of lung cancer are a subject of debate. In the present study, it was found that the expression of miR-191 was significantly upregulated in non‑small cell lung cancer (NSCLC) cells in patients in vivo...
January 4, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28074905/mir-488-inhibits-proliferation-and-cisplatin-sensibility-in-non-small-cell-lung-cancer-nsclc-cells-by-activating-the-eif3a-mediated-ner-signaling-pathway
#9
Chao Fang, Yi-Xin Chen, Na-Yiyuan Wu, Ji-Ye Yin, Xiang-Ping Li, Hsuan-Shun Huang, Wei Zhang, Hong-Hao Zhou, Zhao-Qian Liu
Our previous studied indicated that eukaryotic translation initiation factor 3a (eIF3a) increases the sensitive of platinum-based chemotherapy in lung cancer. MiRNAs play an important role in lung carcinogenesis and drug response. In this study, we aimed to identify potential endogenous miRNAs that inhibit eIF3a expression and determine their influence of this inhibition on cisplatin resistance. Using bioinformatics analysis prediction and confirmation with dual-luciferase reporter assays, we found that miRNA-488 inhibited eIF3a expression by directly binding to the 3'UTR of eIF3a...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28073762/a-dna-intercalation-methodology-for-an-efficient-prediction-of-ligand-binding-pose-and-energetics
#10
Anjali Soni, Pooja Khurana, Tanya Singh, B Jayaram
MOTIVATION: Drug intercalation is an important strategy for DNA inhibition which is often employed in cancer chemotherapy. Despite its high significance, the field is characterized by limited success in identification of novel intercalator molecules and lack of automated and dedicated drug-DNA intercalation methodology. RESULTS: We report here a novel intercalation methodology (christened 'Intercalate') for predicting both the structures and energetics of DNA-intercalator complexes, covering the processes of DNA unwinding and (non-covalent) binding...
January 10, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28073349/the-mir-34a-5p-promotes-the-multi-chemoresistance-of-osteosarcoma-via-repression-of-the-agtr1-gene
#11
Youguang Pu, Fangfang Zhao, Yinpeng Li, Mingda Cui, Haiyan Wang, Xianghui Meng, Shanbao Cai
BACKGROUND: Chemoresistance hinders the curative cancer chemotherapy. MicroRNAs (miRNAs) are key players in diverse biological processes including the chemoresistance of cancers. METHODS: A RNA-seq-based miR-omic analysis of osteosarcoma (OS) cells was performed to detect the levels of miR-34a-5p. Bioinformatics analysis revealed that AGTR1 is one of the target genes of miR-34a-5p. The mRNA and protein levels of AGTR1 were detected in both the miR-34a-5p-mimic transfected G-292 and miR-34a-5p-antagomiR transfected SJSA-1 cells...
January 10, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28072746/znf148-modulates-top2a-expression-and-cell-proliferation-via-cerna-regulatory-mechanism-in-colorectal-cancer
#12
Xian Hua Gao, Juan Li, Yan Liu, Qi Zhi Liu, Li Qiang Hao, Lian Jie Liu, Wei Zhang
BACKGROUND: Competing endogenous RNA (ceRNA) regulation is a novel hypothesized mechanism that states RNA molecules share common target microRNAs (miRNAs) and may competitively combine into the same miRNA pool. METHODS: Zinc finger protein 148 (ZNF148) and TOP2A expression were analyzed in 742 colorectal cancer (CRC) tissues using immunohistochemistry (IHC). ZNF148 mRNA, TOP2A mRNA, miR101, miR144, miR335, and miR365 expression were estimated in 53 fresh frozen CRC tissues by reverse transcription polymerase chain reaction...
January 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28070994/involvement-of-microrna-718-a-new-regulator-of-egr3-in-regulation-of-malignant-phenotype-of-hcc-cells
#13
Zhong-Dong Wang, Fan-Yong Qu, Yuan-Yuan Chen, Zhang-Shen Ran, Hai-Yan Liu, Hai-Dong Zhang
OBJECTIVE: Hepatocellular carcinoma (HCC) is still one of the most common death-related malignancies worldwide. Because the way onset and progression are hidden most, HCC diagnoses are made at an advanced stage, when they are unsuitable for surgical resection. MicroRNAs are a class of small non-coding RNAs, participating in many aspects of cancers. In this study, we tried to establish the role of microRNA-718 (miR-718) in the malignant phenotype of HCC cells and its possible role in HCC diagnosis...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28069592/mir-142-modulates-human-pancreatic-cancer-proliferation-and-invasion-by-targeting-hypoxia-inducible-factor-1-hif-1%C3%AE-in-the-tumor-microenvironments
#14
Yebin Lu, Niandong Ji, Wei Wei, Weijia Sun, Xuejun Gong, Xitao Wang
MicroRNAs regulate most protein-coding genes, including genes important in cancer and other diseases. In our study, we demonstrated that the expression of miR-142 could be significantly suppressed in pancreatic cancer specimens and cell lines, comparing to their adjacent tissues and normal pancreatic cells. Growth and invasion of PANC-1 and SW1990 cells were attenuated by overexpression of miR-142 in vitro With the help of bioinformatics analysis, hypoxia-inducible factor 1 (HIF-1α) was identified to be a direct target of miR-142, and luciferase reporter experiment confirmed this discovery...
January 9, 2017: Biology Open
https://www.readbyqxmd.com/read/28069547/overexpression-of-mir-92a-promotes-the-tumor-growth-of-osteosarcoma-by-suppressing-f-box-and-wd-repeat-containing-protein-7
#15
Xuesheng Jiang, Xiongfeng Li, Fengfeng Wu, Hongliang Gao, Guorong Wang, Hua Zheng, Huajun Wang, Jianyou Li, Chao Chen
MicroRNAs (miRNAs) have been reported to be critical players in osteosarcoma (OS). Among numerous cancer-related miRNAs, the expression level of miR-92a and its potential role in OS has not been investigated. Here, We showed that overexpression of miR-92a was identified in OS specimens and cells compared to normal bone tissues. The high level of miR-92a was correlated with high T classification and advanced clinical stages of OS patients. Notably, miR-92a highly expressing OS patients showed a notably reduced survival rate...
January 6, 2017: Gene
https://www.readbyqxmd.com/read/28069384/regulation-of-cancerous-progression-and-epithelial-mesenchymal-transition-by-mir-34c-3p-via-modulation-of-map3k2-signaling-in-triple-negative-breast-cancer-cells
#16
Jiang Wu, Wei-Zhi Li, Mei-Ling Huang, Hong-Liang Wei, Ting Wang, Jing Fan, Nan-Lin Li, Rui Ling
Emerging but limited data have evidenced an essential involvement of microRNAs (miRNAs) in the development and progression of triple negative breast cancer (TNBC), which empowers these small regulators as an innovative therapeutic approach, especially for this unique tumor subgroup still lacking an efficient and specific therapeutic target. Herein, we reported the down-regulation of miR-34c-3p level in TNBC tissues, and its expression was closely associated with estrogen receptor alpha (ERα), but not other receptors, in well-characterized breast cancer (BCa) cells...
January 6, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28065896/ge-mini-a-mobile-app-for-large-scale-gene-expression-visualization
#17
Zefang Tang, Chenwei Li, Karena Zhang, Mingyu Yang, Xueda Hu
: The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) projects produced large-scale RNA sequencing data, which provides an opportunity for performing integrated expression analysis for all genes across tens of thousands of tumor and normal tissue specimens. Rapid access to and easy visualization of such valuable data could facilitate research in a wide biological area. Here, we present the GE-mini APP for smart phones, a mobile visualization tool for integrated gene expression data based on both TCGA and GTEx...
January 8, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28064384/gut-microbiota-composition-associated-with-alterations-in-cardiorespiratory-fitness-and-psychosocial-outcomes-among-breast-cancer-survivors
#18
Jesseca A Paulsen, Travis S Ptacek, Stephen J Carter, Nianjun Liu, Ranjit Kumar, LaKeshia Hyndman, Elliot J Lefkowitz, Casey D Morrow, Laura Q Rogers
PURPOSE: In this proof-of-concept pilot study, our purpose was to determine correlations between gut microbiota composition and alterations in cardiorespiratory fitness and psychosocial outcomes among post-primary treatment breast cancer survivors (BCS). METHODS: Composition of the gut microbiota in BCS (n = 12) was assessed at baseline (M0) and at the end of 3 months (M3) using Illumina MiSeq DNA Sequencing of the 16S rRNA gene. Gut microbiota composition was analyzed using the QIIME bioinformatics software and represented through diversity metrics and taxa analyses...
January 7, 2017: Supportive Care in Cancer: Official Journal of the Multinational Association of Supportive Care in Cancer
https://www.readbyqxmd.com/read/28062443/a-novel-non-parametric-method-for-uncertainty-evaluation-of-correlation-based-molecular-signatures-its-application-on-pam50-algorithm
#19
Cristóbal Fresno, Germán Alexis González, Gabriela Alejandra Merino, Ana Georgina Flesia, Osvaldo Luis Podhajcer, Andrea Sabina Llera, Elmer Andrés Fernández
MOTIVATION: The PAM50 classifier is used to assign patients to the highest correlated breast cancer subtype irrespectively of the obtained value. Nonetheless, all subtype correlations are required to build the risk of recurrence (ROR) score, currently used in therapeutic decisions. Present subtype uncertainty estimations are not accurate, seldom considered or require a population-based approach for this context. RESULTS: Here we present a novel single-subject non-parametric uncertainty estimation based on PAM50's gene label permutations...
January 6, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28059585/initiation-of-aberrant-dna-methylation-patterns-and-heterogeneity-in-precancerous-lesions-of-human-hepatocellular-cancer
#20
Ryan A Hlady, Dan Zhou, William Puszyk, Lewis R Roberts, Chen Liu, Keith D Robertson
While intratumor heterogeneity contributes to disease progression, metastasis, and resistance to chemotherapy, it also provides a route to understanding the evolution and drivers of disease. Defects in epigenetic landscapes are intimately linked to pathogenesis of a variety of human diseases, with epigenetic deregulation promoting tumorigenesis. Understanding epigenetic heterogeneity is crucial in hepatocellular carcinoma (HCC), where epigenetic alterations are frequent, early, and pathogenic events. We determined genome-wide DNA methylation and copy number variation leveraging the Infinium 450 K in a series of regenerative nodules from within single patient livers...
January 6, 2017: Epigenetics: Official Journal of the DNA Methylation Society
keyword
keyword
67613
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"