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https://www.readbyqxmd.com/read/28652254/oviductal-microvesicles-and-their-effect-on-in-vitro-maturation-of-canine-oocytes
#1
Anna Lange-Consiglio, Claudia Perrini, Giulia Albini, Silvia Modina, Valentina Lodde, Eleonora Orsini, Paola Esposti, Fausto Cremonesi
The effect of conditioned medium (CM) or microvesicles (MVs), secreted by multicellular spheroids of oviductal cells, and the involvement of some microRNAs (miRNAs) were investigated in canine oocyte maturation. To generate CM, spheroids were cultured for 3 days. MVs were obtained by ultracentrifugation of CM at 100,000 g and measured for size and concentration by NanoSight instrument. Cumulus-oocyte complexes (COCs) were matured at 38.5°C with 5% CO2 and 5% of O2 in synthetic oviductal fluid (SOF) in biphasic systems: for 24 h, with 5...
August 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28638226/validation-of-a-serum-microrna-panel-as-biomarkers-for-early-diagnosis-of-hepatocellular-carcinoma-post-hepatitis-c-infection-in-egyptian-patients
#2
Moustafa Nouh Elemeery, Ahmed Noah Badr, Marwa Anwar Mohamed, Doaa Ahmed Ghareeb
AIM: To investigate the prospective importance of serum micro (mi)RNAs (miR-125b, miR-138b, miR-1269, miR-214-5p, miR-494, miR375 and miR-145) as early biomarkers for the diagnosis of hepatitis C virus (HCV)-related hepatocellular carcinoma (HCC). METHODS: Two-hundred and fifty HCV4a patients, 224 HCV4a-HCC patients, and 84 healthy controls were enrolled in the study. Expression levels of miR214-5p, miR-125b, miR-1269 and miR-375 were quantified using quantitative real-time PCR...
June 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28628844/serum-micrornas-in-buffalo-cows-potential-biomarkers-of-pregnancy
#3
Gabriella Guelfi, Valentina Stefanetti, Silvio De Luca, Andrea Giontella, Vittoria Lucia Barile, Olimpia Barbato
MiRNAs (microRNA) constitute a large family of single-stranded, non-coding small RNAs. Although the functions and target genes of most miRNAs are still unknown, it has been well established that they are involved in embryogenesis, organogenesis and neonatal birth. In recent decades, interest in buffalo breeding has largely increased worldwide thus focusing the attention on this species as a dairy purpose animal. Problems related to long calving interval, late puberty and seasonal anestrus hamper reproductive efficiency in this species...
June 4, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28627030/mir-375-slc7a11-axis-regulates-oral-squamous-cell-carcinoma-proliferation-and-invasion
#4
Yadong Wu, Xiangjie Sun, Bin Song, Xiaoling Qiu, Jianjiang Zhao
We aimed to detect the functions of miR-375/SLC7A11 axis on oral squamous cell carcinoma (OSCC) cell proliferation and invasion. Expression levels of miR-375 and SLC7A11 in OSCC tissues and cells were measured with RT-qPCR and western blot. Targeting site was predicted by TargetScan and confirmed by dual luciferase reporting assay. By way of manipulating the expression level of miR-375 and SLC7A11 in CAL-27 and Tca8113 cell lines, the cell biological abilities were evaluated. MTT, colony formation, Transwell, wound healing assays and flow cytometry were used to detect OSCC cell viability, proliferation, invasion, migration and apoptosis, respectively...
June 19, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28624193/mir-375-and-doxorubicin-co-delivered-by-liposomes-for-combination-therapy-of-hepatocellular-carcinoma
#5
Yin-Ping Fan, Jia-Zhi Liao, Ya-Qi Lu, De-An Tian, Feng Ye, Peng-Xuan Zhao, Guang-Ya Xiang, Wang-Xian Tang, Xing-Xing He
Doxorubicin (DOX) is one of the most frequently used anti-cancer drugs and the front line option for hepatocellular carcinoma (HCC) treatment. However, the clinical applications of DOX are restricted largely due to its toxicity and chemoresistance. Here, we report that miR-375 and DOX were co-delivered by liposomes (named L-miR-375/DOX-NPs) for combination therapy of HCC and drug resistance reversion of DOX. In vitro, L-miR-375/DOX-NPs could deliver DOX and miR-375 efficiently and simultaneously into HCC cells and ensure the successful release of mature miR-375 and DOX...
June 16, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28618945/the-expression-profiling-of-serum-mir-92a-mir-375-and-mir-760-in-colorectal-cancer-an-egyptian-study
#6
Ahmed Elshafei, Olfat Shaker, Ossama Abd El-Motaal, Tarek Salman
Dysregulation in microRNA expression is a common feature in colorectal cancer. Due to the inconsistent results regarding serum miR-92a expression pattern and the insufficient studies on serum miR-375 and miR-760, we aimed in this study to investigate their expression profile and diagnostic and prognostic power in Egyptian colorectal cancer patients. The expression profile of miR-92a, miR-375, and miR-760 was determined in the sera of 64 colorectal cancer patients using quantitative real-time reverse transcription polymerase chain reaction in comparison to 27 healthy control subjects...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28603428/upregulation-of-mir-375-level-ameliorates-morphine-analgesic-tolerance-in-mouse-dorsal-root-ganglia-by-inhibiting-the-jak2-stat3-pathway
#7
Haiqin Li, Rong Tao, Jing Wang, Lingjie Xia
Several lines of evidence indicate that microRNAs (miRNAs) modulate tolerance to the analgesic effects of morphine via regulation of pain-related genes, making dysregulation of miRNA levels a clinical target for controlling opioid tolerance. However, the precise mechanisms by which miRNAs regulate opioid tolerance are unclear. In the present study, we noted that the miR-375 level was downregulated but the expression of Janus kinase 2 (JAK2) was upregulated in mouse dorsal root ganglia (DRG) following chronic morphine treatment...
2017: Journal of Pain Research
https://www.readbyqxmd.com/read/28599478/microrna-375-suppresses-esophageal-cancer-cell-growth-and-invasion-by-repressing-metadherin-expression
#8
Changmei Hu, Liang Lv, Jie Peng, Deliang Liu, Xuehong Wang, Yuqian Zhou, Jirong Huo
Accumulating evidence indicates that aberrant expression of microRNAs is involved in tumorigenesis, tumor progression and response to therapy. MicroRNA-375 (miR-375) is an important cancer-associated RNA that is downregulated in multiple types of cancer. In the present study, the potential effects of and underlying molecular mechanism for miR-375 in esophageal cancer were investigated. The expression of miR-375 in paired esophageal squamous cell carcinoma (ESCC) and non-tumor tissues from 10 patients was quantified using the reverse transcription-quantitative polymerase chain reaction...
June 2017: Oncology Letters
https://www.readbyqxmd.com/read/28597695/micrornas-in-hpv-associated-cancers-small-players-with-big-consequences
#9
Sandeep Satapathy, Jyotsna Batra, Varinder Jeet, Erik W Thompson, C Punyadeera
MicroRNAs (miRs) are short (~20 nucleotides) non-coding ribonuecleic acids (ncRNAs) known to be involved in cellular processes such as proliferation, differentiation, immune response, pathogenicity and tumourigenesis, among many others. The regulatory mechanisms exerted by miRs have been implicated in many cancers, including Human Papillomavirus (HPV)-associated cancers. Areas covered: In this review, the authors discuss the involvement of miRs (-143, -375, -21, -200, -296 etc.) that have been shown to be dysregulated in HPV-associated cancers...
July 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28577837/mir-375-delivered-by-lipid-coated-doxorubicin-calcium-carbonate-nanoparticles-overcomes-chemoresistance-in-hepatocellular-carcinoma
#10
Pengxuan Zhao, Shuangping Wu, Yao Cheng, Jia You, Yan Chen, Minsi Li, Chuanchuan He, Xiaojuan Zhang, Tan Yang, Yao Lu, Robert J Lee, Xingxing He, Guangya Xiang
Hepatocellular carcinoma (HCC) is a prevalent and lethal disease that is characterized by drug resistance. Doxorubicin (DOX) is a widely used chemotherapeutic drug and miR-375 has been shown to be a tumor suppressor in HCC. Here, we reported that miR-375 and DOX co-loaded into lipid-coated calcium carbonate nanoparticles (LCC-DOX/miR-375 NPs), enhanced the anti-tumor effects through combination therapy, and were highly effective in reversing drug resistance in HCC. LCC-DOX/miR-375 NPs were prepared by a reverse microemulsions method...
June 1, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28572713/specific-gene-and-microrna-expression-pattern-contributes-to-the-epithelial-to-mesenchymal-transition-in-a-rat-model-of-experimental-colitis
#11
Éva Boros, Marianna Csatári, Csaba Varga, Balázs Bálint, István Nagy
The aim of this study was to determine the gene- and microRNA-expression profile contributing to epithelial to mesenchymal transition in a rat model of experimental colitis. For this, inflammation was induced by injecting 2,4,6-trinitrobenzene sulphonic acid to the colon of male Wistar rats. Samples were taken from both inflamed and uninflamed regions of the same colon, total RNA was isolated, and the mRNA and microRNA expressions were monitored. We have determined that the expression of genes responsible for inducing mesenchymal phenotype, such as Egr1, Fgf2, Fgf7, Jak2, Notch2, Hif1α, Zeb2, Mmp9, Lox, and Vim, was all significantly induced in the inflamed regions of the affected colons while the epithelial marker E-cadherin (Cdh1) was downregulated...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28533502/clinical-value-and-prospective-pathway-signaling-of-microrna-375-in-lung-adenocarcinoma-a-study-based-on-the-cancer-genome-atlas-tcga-gene-expression-omnibus-geo-and-bioinformatics-analysis
#12
Ting-Qing Gan, Wen-Jie Chen, Hui Qin, Su-Ning Huang, Li-Hua Yang, Ye-Ying Fang, Lin-Jiang Pan, Zu-Yun Li, Gang Chen
BACKGROUND Lung adenocarcinoma (LUAD) is the most frequent lung cancer. MicroRNAs (miRNAs) are believed to have fundamental roles in tumorigenesis of LUAD. Although miRNAs are broadly recognized in LUAD, the role of microRNA-375 in LUAD is still not fully elucidated. MATERIAL AND METHODS We evaluated the significance of miR-375 expression in LUAD by using analysis of a public dataset from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database and a literature review. Furthermore, we investigated the biological function of miR-375 by gene ontology enrichment and target prediction analysis...
May 23, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28521287/5azadc-treatment-upregulates-mir-375-level-and-represses-hpv16-e6-expression
#13
Adrien Morel, Aurélie Baguet, Jérôme Perrard, Caroline Demeret, Elise Jacquin, David Guenat, Christiane Mougin, Jean-Luc Prétet
High risk human papillomaviruses are the etiological agents of cervical cancer and HPV16 is the most oncogenic genotype. Immortalization and transformation of infected cells requires the overexpression of the two viral oncoproteins E6 and E7 following HPV DNA integration into the host cell genome. Integration often leads to the loss of the E2 open reading frame and the corresponding protein can no longer act as a transcriptional repressor on p97 promoter. Recently, it has been proposed that long control region methylation also contributes to the regulation of E6/E7 expression...
May 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28515914/analysis-of-the-function-of-microrna-375-in-humans-using-bioinformatics
#14
Xiaohua Chen, Baoxia Li, Rongcheng Luo, Sina Cai, Cao Zhang, Xiaolong Cao
MicroRNA-375 (miR-375) is expressed at low levels in many types of solid tumor, particularly in gastrointestinal tumors. It is considered to be important in the development of cancer and certain diseases. Thus, more detailed knowledge is required on the particular functions of miR-375. miRs function by regulating target genes. Therefore, in the current study, miRWalk (which includes the data from 10 prediction software programs) was used to predict the target genes of miR-375. The genes, which were co-predicted using five different software programs were further analyzed using Database for Annotation, Visualization and Integrated Discovery online software [including gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis]...
May 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28499703/mir-375-regulates-invasion-related-proteins-vimentin-and-l-plastin
#15
Lizandra Jimenez, Jihyeon Lim, Berta Burd, Thomas M Harris, Thomas J Ow, Nicole Kawachi, Thomas J Belbin, Ruth Angeletti, Michael B Prystowsky, Geoffrey Childs, Jeffrey E Segall
Invasion is a hallmark of advanced head and neck squamous cell carcinoma (HNSCC). We previously determined that low relative miR-375 expression was associated with poor patient prognosis. HNSCC cells with increased miR-375 expression have lower invasive properties and impaired invadopodium activity. Using stable isotope labeling with amino acids in cell culture and reverse-phase liquid chromatography mass spectrometry, we assessed the impact of miR-375 expression on protein levels in UM-SCC-1 cells. Increased miR-375 expression was associated with down-regulation of proteins involved in cellular assembly and organization, death and survival, and movement...
July 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28490741/circulating-mirna-21-5p-as-a-diagnostic-biomarker-for-pancreatic-cancer-evidence-from-comprehensive-mirna-expression-profiling-analysis-and-clinical-validation
#16
Kai Qu, Xing Zhang, Ting Lin, Tian Liu, Zhixin Wang, Sushun Liu, Lei Zhou, Jichao Wei, Hulin Chang, Ke Li, Zheng Wang, Chang Liu, Zheng Wu
Pancreatic cancer (PC) is a highly fatal disease worldwide and is often misdiagnosed in its early stages. The exploration of novel non-invasive biomarkers will definitely benefit PC patients. Recently, circulating miRNAs in body fluids are emerging as non-invasive biomarkers for PC diagnosis. In this study, we first conducted comprehensive robust rank aggregation (RRA) analysis based on 21 published miRome profiling studies. We statistically identified and clinically validated a miRNA expression pattern in PC patients...
May 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28483594/differences-in-the-microrna-profiles-of-subcutaneous-adipose-derived-stem-cells-and-omental-adipose-derived-stem-cells
#17
Feihu Hu, Peng Xu, Bo Sun, Zhongdang Xiao
Adipose-derived stem cells (ASCs) isolated from subcutaneous (SC) and omentum (O) share similar characteristics, but the differences in their microRNA profiles are mostly unknown. In this study, besides significant differences in cell morphology and the differentiation ability of the two types of ASCs, the microRNA expression profiles of the cell lines were determined using SOLiD next-generation sequencing. The in-depth analysis found that miR-214, miR-222, miR-181a, miR-26a and miR-23/27/24 clusters and miR-375 act as "markers" to distinguish the different fat deposit-derived ASCs...
August 20, 2017: Gene
https://www.readbyqxmd.com/read/28471299/interleukin-10-deficiency-impairs-reparative-properties-of-bone-marrow-derived-endothelial-progenitor-cell-exosomes
#18
Yujia Yue, Venkata Naga Srikanth Garikipati, Suresh Kumar Verma, David A Goukassian, Raj Kishore
Endothelial progenitor cell (EPC)-based therapy has immense potential to promote cardiac neovascularization and attenuate ischemic injury. Functional benefits of EPCs and other adult stem cell therapies largely involve paracrine mechanisms and exosomes secreted by stem cells are emerging as pivotal paracrine entity of stem/progenitor cells. However, modest outcomes after EPC-/stem cell-based clinical trials suggest that stem cell/exosome function might be modulated by stimuli they encounter in ischemic tissues, including systemic inflammation...
June 15, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28470118/changes-in-high-density-lipoprotein-carried-mirna-contribution-to-the-plasmatic-pool-after-consumption-of-dietary-trans-fat-in-healthy-men
#19
Véronique Desgagné, Renée Guérin, Simon-Pierre Guay, François Corbin, Patrick Couture, Benoit Lamarche, Luigi Bouchard
AIM: High-density lipoproteins (HDLs) are associated to cardioprotection and transport functional miRNAs in circulation. The aim of this study is to assess whether consumption of trans fatty acids (TFAs) modifies the HDL-carried miRNA concentration and their contribution to the plasmatic pool. METHODS: In a double-blind, randomized crossover controlled study, nine healthy men were fed each of three isoenergetic 4-week diets: first, rich in industrial TFAs; second, rich in TFAs from ruminants; third, low in TFAs...
May 4, 2017: Epigenomics
https://www.readbyqxmd.com/read/28449331/mir-130a-as-a-diagnostic-marker-to-differentiate-malignant-mesothelioma-from-lung-adenocarcinoma-in-pleural-effusion-cytology
#20
Rocco Cappellesso, Marco Galasso, Lorenzo Nicolè, Paolo Dabrilli, Stefano Volinia, Ambrogio Fassina
BACKGROUND: Malignant pleural mesothelioma is a rare tumor with a dismal prognosis, usually presenting with recurrent effusions. However, the majority of malignant pleural effusions are due to lung adenocarcinoma (AdC). The distinction between these tumors has considerable therapeutic and medicolegal implications and can be very challenging both histologically and cytologically. Appropriate immunohistochemistry (IHC) is required to support the diagnosis. MicroRNA (miRNA) expression analysis could be a viable diagnostic tool for distinguishing between these tumors...
April 27, 2017: Cancer
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