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https://www.readbyqxmd.com/read/28448606/a-feeding-protocol-for-delivery-of-agents-to-assess-development-in-varroa-mites
#1
Ana R Cabrera, Paul D Shirk, Peter E A Teal
A novel feeding protocol for delivery of bio-active agents to Varroa mites was developed by providing mites with honey bee larva hemolymph supplemented with cultured insect cells and selected materials delivered on a fibrous cotton substrate. Mites were starved, fed on treated hemolymph to deliver selected agents and then returned to bee larvae. Transcript levels of two reference genes, actin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), as well as for nine selected genes involved in reproductive processes showed that the starvation and feeding protocol periods did not pose a high level of stress to the mites as transcript levels remained comparable between phoretic mites and those completing the protocol...
2017: PloS One
https://www.readbyqxmd.com/read/28439742/exploration-of-the-binding-affinities-between-ecdysone-agonists-and-ecr-usp-by-docking-and-mm-pb-gbsa-approaches
#2
Xueping Hu, Jin Xie, Song Hu, Li Zhang, Yanhong Dong
Ecdysone receptor (EcR) is a significant target in the identification of new environmentally friendly pesticides. There are two types of ecdysone agonists: steroidal ecdysone agonists and dibenzoylhydrazines (DBHs). In this study, various modeling methods (homology modeling, molecular docking, MD simulation, binding free energy calculation, and per-residue binding free energy decomposition) were utilized to study the different binding mechanisms of two types of ecdysone agonists. Our theoretical results indicated that the relative binding potencies of DBHs can be ranked sufficiently accurately using the MOE docking method...
May 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/28439025/endocrine-network-essential-for-reproductive-success-in-drosophila-melanogaster
#3
Matthew Meiselman, Sang Soo Lee, Raymond-Tan Tran, Hongjiu Dai, Yike Ding, Crisalejandra Rivera-Perez, Thilini P Wijesekera, Brigitte Dauwalder, Fernando Gabriel Noriega, Michael E Adams
Ecdysis-triggering hormone (ETH) was originally discovered and characterized as a molt termination signal in insects through its regulation of the ecdysis sequence. Here we report that ETH persists in adult Drosophila melanogaster, where it functions as an obligatory allatotropin to promote juvenile hormone (JH) production and reproduction. ETH signaling deficits lead to sharply reduced JH levels and consequent reductions of ovary size, egg production, and yolk deposition in mature oocytes. Expression of ETH and ETH receptor genes is in turn dependent on ecdysone (20E)...
April 24, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28428023/cyp450s-analysis-across-spiny-lobster-metamorphosis-identifies-a-long-sought-missing-link-in-crustacean-development
#4
Tomer Ventura, Utpal Bose, Quinn P Fitzgibbon, Gregory G Smith, P Nicholas Shaw, Scott F Cummins, Abigail Elizur
Cytochrome P450s (CYP450s) are a rapidly evolving family of enzymes, making it difficult to identify bona fide orthologs with notable lineage-specific exceptions. In ecdysozoans, a small number of the most conserved orthologs include enzymes which metabolize ecdysteroids. Ecdysone pathway components were recently shown in a decapod crustacean but with a notable absence of shade, which is important for converting ecdysone to its active form, 20-hydroxyecdysone (20HE), suggesting that another CYP450 performs a similar function in crustaceans...
April 17, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28414820/transcriptome-analysis-of-the-epidermis-of-the-purple-quail-like-q-lp-mutant-of-silkworm-bombyx-mori
#5
Pingyang Wang, Zhiyong Qiu, Dingguo Xia, Shunming Tang, Xingjia Shen, Qiaoling Zhao
A new purple quail-like (q-lp) mutant found from the plain silkworm strain 932VR has pigment dots on the epidermis similar to the pigment mutant quail (q). In addition, q-lp mutant larvae are inactive, consume little and grow slowly, with a high death rate and other developmental abnormalities. Pigmentation of the silkworm epidermis consists of melanin, ommochrome and pteridine. Silkworm development is regulated by ecdysone and juvenile hormone. In this study, we performed RNA-Seq on the epidermis of the q-lp mutant in the 4th instar during molting, with 932VR serving as the control...
2017: PloS One
https://www.readbyqxmd.com/read/28408149/leptinotarsa-cap-n-collar-isoform-c-kelch-like-ech-associated-protein%C3%A2-1-signaling-is-critical-for-the-regulation-of-ecdysteroidogenesis-in-the-larvae
#6
Qiang-Kun Sun, Qing-Wei Meng, Qing-Yu Xu, Pan Deng, Wen-Chao Guo, Guo-Qing Li
Drosophila cap 'n' collar isoform C (CncC) and Kelch-like ECH associated protein 1 (Keap1) regulate metamorphosis by transcriptional control of a subset of genes involved in ecdysteroidogenesis, 20-hydroxyecdysone (20E) signaling, and juvenile hormone (JH) degradation. In the present paper, we found that prothoracicotropic hormone signal was required for the activation of LdCncC and LdKeap1 in Leptinotarsa decemlineata. Moreover, RNA interference of LdCncC or LdKeap1 in the fourth-instar larvae delayed development...
April 10, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28394252/steroid-hormone-induction-of-temporal-gene-expression-in-drosophila-brain-neuroblasts-generates-neuronal-and-glial-diversity
#7
Mubarak Hussain Syed, Brandon Mark, Chris Q Doe
An important question in neuroscience is how stem cells generate neuronal diversity. During Drosophila embryonic development, neural stem cells (neuroblasts) sequentially express transcription factors that generate neuronal diversity; regulation of the embryonic temporal transcription factor cascade is lineage-intrinsic. In contrast, larval neuroblasts generate longer ~50 division lineages, and currently only one mid-larval molecular transition is known: Chinmo/Imp/Lin-28+ neuroblasts transition to Syncrip+ neuroblasts...
April 10, 2017: ELife
https://www.readbyqxmd.com/read/28388784/beta-ecdysone-protects-mouse-osteoblasts-from-glucocorticoid-induced-apoptosis-in-vitro
#8
Wei-Wei Dai, Li-Bo Wang, Guo-Qin Jin, Hong-Jin Wu, Jie Zhang, Cheng-Long Wang, Yuan-Ji Wei, Joon-Ho Lee, Yu-An Evan Lay, Wei Yao
Glucocorticoid-induced osteoporosis is a common form of secondary osteoporosis. Glucocorticoids affect both bone formation and resorption, and prolonged glucocorticoid exposure can suppress osteoblast activities. beta-Ecdysone, found in many plants, is involved in protein synthesis, carbohydrate and lipid metabolism, and immunologic modulation. Here, we evaluated the effects of beta-ecdysone on osteoblast viability by assessing apoptosis following treatment with excess glucocorticoids. Mouse bone marrow stromal cells were induced to differentiate and grow into osteoblasts, and then treated with 10 µM glucocorticoid and 10, 1, or 0...
April 7, 2017: Planta Medica
https://www.readbyqxmd.com/read/28388441/memory-is-the-treasury-and-guardian-of-all-things
#9
Agustina D'Urso, Jason H Brickner
Transcriptional memory often relies on interactions with nuclear pore proteins. In this issue of Molecular Cell, Pascual-Garcia et al. (2017) describe hormone-induced developmental transcriptional memory in cells that have previously experienced ecdysone, mediated by Nup98-dependent enhancer-promoter looping.
April 6, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28385724/on-gonads-and-gadflies-the-estrus-angle
#10
Stephen G Hillier
The first sex steroid to be crystallized was the vertebrate ovarian hormone, estrone - a less potent metabolite of 17βestradiol, which in mammals stimulates the female urge to mate (estrus). The gadfly (Greek oistros) lent its name to the process of estrus, as an insect that bites and torments in classical Greek mythology. With the crystallization of a moult-inducing steroid (ecdysone) from insects, an interesting parallel emerged between mating and moulting in mammals and arthropods. Ecdysterone (potent ecdysone metabolite) has anabolic effects in mammalian muscle cells that can be blocked by selective estrogen receptor-antagonists...
April 6, 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/28378812/ecr-recruits-dmi-2-and-increases-efficiency-of-dmi-2-mediated-remodelling-to-constrain-transcription-of-hormone-regulated-genes
#11
Judith Kreher, Kristina Kovač, Karim Bouazoune, Igor Mačinković, Anna Luise Ernst, Erik Engelen, Roman Pahl, Florian Finkernagel, Magdalena Murawska, Ikram Ullah, Alexander Brehm
Gene regulation by steroid hormones plays important roles in health and disease. In Drosophila, the hormone ecdysone governs transitions between key developmental stages. Ecdysone-regulated genes are bound by a heterodimer of ecdysone receptor (EcR) and Ultraspiracle. According to the bimodal switch model, steroid hormone receptors recruit corepressors in the absence of hormone and coactivators in its presence. Here we show that the nucleosome remodeller dMi-2 is recruited to ecdysone-regulated genes to limit transcription...
April 5, 2017: Nature Communications
https://www.readbyqxmd.com/read/28369111/larval-diapause-termination-in-the-bamboo-borer-omphisa-fuscidentalis
#12
Suphawan Suang, Manaporn Manaboon, Tippawan Singtripop, Kiyoshi Hiruma, Yu Kaneko, Pimonrat Tiansawat, Peter Neumann, Panuwan Chantawannakul
In insects, juvenile hormone (JH) and 20-hydroxyecdysone (20E) regulate larval growth and molting. However, little is known about how this cooperative control is terminating larval diapause especially in the bamboo borer, Omphisa fuscidentalis. In both in vivo and in vitro experiments, we here measured the expression levels of genes which were affected by juvenile hormone analogue (JHA: S-methoprene) and 20-hydroxyecdysone (20E) in diapausing O. fuscidentalis larvae. Corresponding mRNA expression changes in the subesophageal ganglion (SG) and prothoracic gland (PG) were evaluated using qRT-PCR...
2017: PloS One
https://www.readbyqxmd.com/read/28366641/metazoan-nuclear-pores-provide-a-scaffold-for-poised-genes-and-mediate-induced-enhancer-promoter-contacts
#13
Pau Pascual-Garcia, Brian Debo, Jennifer R Aleman, Jessica A Talamas, Yemin Lan, Nha H Nguyen, Kyoung J Won, Maya Capelson
Nuclear pore complex components (Nups) have been implicated in transcriptional regulation, yet what regulatory steps are controlled by metazoan Nups remains unclear. We identified the presence of multiple Nups at promoters, enhancers, and insulators in the Drosophila genome. In line with this binding, we uncovered a functional role for Nup98 in mediating enhancer-promoter looping at ecdysone-inducible genes. These genes were found to be stably associated with nuclear pores before and after activation. Although changing levels of Nup98 disrupted enhancer-promoter contacts, it did not affect ongoing transcription but instead compromised subsequent transcriptional activation or transcriptional memory...
April 6, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28365999/proteome-analysis-of-drosophila-mutants-identifies-a-regulatory-role-for-14-3-3%C3%AE%C2%B5-in-metabolic-pathways
#14
Yeap S Ng, Alexandra Sorvina, Christie A Bader, Florian Weiland, Angel F Lopez, Peter Hoffmann, Tetyana Shandala, Douglas A Brooks
The evolutionary conserved family of 14-3-3 proteins appears to have a role in integrating numerous intracellular pathways, including signal transduction, intracellular trafficking, and metabolism. However, little is known about how this interactive network might be affected by the direct abrogation of 14-3-3 function. The loss of Drosophila 14-3-3ε resulted in reduced survival of mutants during larval-to-adult transition, which is known to depend on an energy supply coming from the histolysis of fat body tissue...
April 13, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28355071/ecdysone-receptor-agonism-leading-to-lethal-molting-disruption-in-arthropods-review-and-adverse-outcome-pathway-development
#15
You Song, Daniel L Villeneuve, Kenji Toyota, Taisen Iguchi, Knut Erik Tollefsen
Molting is critical for growth, development, reproduction, and survival in arthropods. Complex neuroendocrine pathways are involved in the regulation of molting and may potentially become targets of environmental endocrine disrupting chemicals (EDCs). Based on several known ED mechanisms, a wide range of pesticides has been developed to combat unwanted organisms in food production activities such as agriculture and aquaculture. Meanwhile, these chemicals may also pose hazards to nontarget species by causing molting defects, and thus potentially affecting the health of the ecosystems...
April 10, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28317899/minocycline-treatment-suppresses-juvenile-development-and-growth-by-attenuating-insulin-tor-signaling-in-drosophila-animal-model
#16
Hyun Myoung Yun, Sujin Noh, Seogang Hyun
Minocycline is a broad spectrum, semi-synthetic tetracycline analog that is used to treat bacterial infection. Recently, this drug has been receiving increasing attention for its non-antibiotic properties, including anti-inflammatory, tumor suppressive, and neuroprotective effects. Drosophila is a useful model organism for studying human metabolism and disease. In this study, we investigated the effects of minocycline on juvenile development and growth in Drosophila. Feeding minocycline to Drosophila larvae suppresses larval body growth and delays the timing of pupation in a dose-dependent manner...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28292900/hormone-and-receptor-interplay-in-the-regulation-of-mosquito-lipid-metabolism
#17
Xueli Wang, Yuan Hou, Tusar T Saha, Gaofeng Pei, Alexander S Raikhel, Zhen Zou
Mosquitoes transmit devastating human diseases because they need vertebrate blood for egg development. Metabolism in female mosquitoes is tightly coupled with blood meal-mediated reproduction, which requires an extremely high level of energy consumption. Functional analysis has shown that major genes encoding for enzymes involved in lipid metabolism (LM) in the mosquito fat bodies are down-regulated at the end of the juvenile hormone (JH)-controlled posteclosion (PE) phase but exhibit significant elevation in their transcript levels during the post-blood meal phase (PBM), which is regulated mainly by 20-hydroxyecdysone (20E)...
March 28, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28282622/the-effects-of-fipronil-and-the-photodegradation-product-fipronil-desulfinyl-on-growth-and-gene-expression-in-juvenile-blue-crabs-callinectes-sapidus-at-different-salinities
#18
Andrew D Goff, Parichehr Saranjampour, Lauren M Ryan, Michelle L Hladik, Joseph A Covi, Kevin L Armbrust, Susanne M Brander
Endocrine disrupting compounds (EDCs) are now widely established to be present in the environment at concentrations capable of affecting wild organisms. Although many studies have been conducted in fish, less is known about effects in invertebrates such as decapod crustaceans. Decapods are exposed to low concentrations of EDCs that may cause infertility, decreased growth, and developmental abnormalities. The objective herein was to evaluate effects of fipronil and its photodegradation product fipronil desulfinyl...
February 28, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28260683/endocrine-related-genes-are-altered-by-antibacterial-agent-triclosan-in-chironomus-riparius-aquatic-larvae
#19
Pedro Martínez-Paz, Mónica Morales, Josune Urien, Gloria Morcillo, José Luis Martínez-Guitarte
Triclosan (TCS) is an antibacterial agent widely used in personal care and consumer products and commonly detected in aquatic ecosystems. In the present study, the effects of TCS on endocrine-related genes of Chironomus riparius aquatic larvae, a reference organism in aquatic toxicology, were evaluated. Twenty-four-hour in vivo exposures at 10µg/L, 100µg/L, and 1000µg/L TCS revealed that this xenobiotic was able to alter the transcriptional activity of ecdysone receptor gene (EcR), the ultraspiracle gene (usp), the estrogen-related receptor gene (ERR), and the E74 early ecdysone-inducible gene, as measured by real-time RT-PCR...
March 2, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28257485/deep-sequencing-of-the-prothoracic-gland-transcriptome-reveals-new-players-in-insect-ecdysteroidogenesis
#20
Takayoshi Nakaoka, Masatoshi Iga, Tetsuya Yamada, Ikumi Koujima, Mika Takeshima, Xiangying Zhou, Yutaka Suzuki, Mari H Ogihara, Hiroshi Kataoka
Ecdysteroids are steroid hormones that induce molting and determine developmental timing in arthropods. In insect larva, the prothoracic gland (PG) is a major organ for ecdysone synthesis and release. Released ecdysone is converted into the active form, 20-hydroxyecdysone (20E) in the peripheral tissues. All processes from ecdysone synthesis and release from the PG to its conversion to 20E are called ecdysteroidogenesis and are under the regulation of numerous factors expressed in the PG and peripheral tissues...
2017: PloS One
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