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https://www.readbyqxmd.com/read/28207159/the-critical-role-of-the-znf217-oncogene-in-promoting-breast-cancer-metastasis-to-the-bone
#1
Aurélie Bellanger, Caterina F Donini, Julie A Vendrell, Jonathan Lavaud, Irma Machuca-Gayet, Maëva Ruel, Julien Vollaire, Evelyne Grisard, Balázs Győrffy, Ivan Bièche, Olivier Peyruchaud, Jean-Luc Coll, Isabelle Treilleux, Véronique Maguer-Satta, Véronique Josserand, Pascale A Cohen
Bone metastasis affects more than 70% of patients with advanced breast cancer. However, the molecular mechanisms underlying this process remain unclear. Based on the analysis of clinical datasets, and in vitro and in vivo experiments, we report that the ZNF217 oncogene is a crucial mediator and indicator of bone metastasis. Patients with high ZNF217 mRNA expression levels in primary breast tumours had a higher risk of developing bone metastases. MDA-MB-231 breast cancer cells stably transfected with ZNF217 (MDA-MB-231-ZNF217) displayed the dysregulated expression of a set of genes with bone homing and metastasis characteristics, which overlapped with two previously described "osteolytic bone metastasis" gene signatures, while also highlighting the bone morphogenetic protein (BMP) pathway...
February 16, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28149772/differentiation-potential-of-human-adipose-tissue-derived-stem-cells-into-photoreceptors-through-explants-culture-and-enzyme-methods
#2
Wei-Wei Xu, Li Huang, Kelvin K L Chong, Doreen S Y Leung, Benjamin F L Li, Zheng-Qin Yin, Yi-Fei Huang, Chi Pui Pang
AIM: To investigate the retinal photoreceptor differentiation potential of human orbital adipose tissue-derived stem cells (ADSCs) generated by enzyme (EN) and explant (EX) culture methods. METHODS: We investigated potentials of human orbital ADSCs to differentiate into photoreceptors through EN and EX culture methods. EN and EX orbital ADSCs were obtained from the same donor during rehabilitative orbital decompression, and then were subject to a 3-step induction using Noggin, DKK-1, IGF-1 and b-FGF at different time points for 38d...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28125332/the-versatility-of-rhoa-activities-in-neural-differentiation
#3
Arie Horowitz, Junning Yang, Jingli Cai, Lorraine Iacovitti
In this commentary we discuss a paper we published recently on the activities of the GTPase RhoA during neural differentiation of murine embryonic stem cells, and relate our findings to previous studies. We narrate how we found that RhoA impedes neural differentiation by inhibiting the production as well as the secretion of noggin, a soluble factor that antagonizes bone morphogenetic protein. We discuss how the questions we tried to address shaped the study, and how embryonic stem cells isolated from a genetically modified mouse model devoid of Syx, a RhoA-specific guanine exchange factor, were used to address them...
January 26, 2017: Small GTPases
https://www.readbyqxmd.com/read/28096456/antibody-conjugated-dna-based-nanocarriers-intercalated-with-doxorubicin-eliminate-myofibroblasts-in-explants-of-human-lens-tissue
#4
Jacquelyn Gerhart, Marvin Greenbaum, Lou Casta, Anthony Clemente, Keith Mathers, Robert Getts, Mindy George-Weinstein
Posterior capsule opacification (PCO) occurs in some adults and most children following cataract surgery. The fibrotic form of PCO arises, in part, from migratory, contractile myofibroblasts that deform the lens capsule and impair vision. In short-term cultures of human anterior lens tissue, myofibroblasts emerge from Myo/Nog cells that are identified with the G8 monoclonal antibody and by their expression of the MyoD transcription factor and bone morphogenetic protein inhibitor noggin. In this study, we tested the hypothesis that targeted depletion of Myo/Nog cells with the G8 mAb conjugated to three-dimensional DNA nanocarriers intercalated with Doxorubicin (G8:3DNA:Dox) would prevent the accumulation of myofibroblasts in long-term, serum and growth factor free cultures of human lens tissue obtained by capsulorhexis...
January 17, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/28095743/adult-murine-pancreatic-progenitors-require-epidermal-growth-factor-and-nicotinamide-for-self-renewal-and-differentiation-in-a-serum-and-conditioned-medium-free-culture
#5
Lena Wedeken, Angela Luo, Jacob R Tremblay, Jeffrey Rawson, Liang Jin, Dan Gao, Janine Quijano, Hsun Teresa Ku
Adult pancreatic stem and progenitor cells may serve as an alternative source of insulin-secreting endocrine cells in cell replacement therapy for type 1 diabetes, but much remained unknown about these cells. We previously identified adult murine pancreatic progenitor-like cells that displayed in vitro self-renewal and tri-lineage differentiation activities in a three-dimensional colony/organoid assay containing 1% methylcellulose and 5% Matrigel. However, the presence of other undefined culture components, such as serum and conditioned medium, has prevented a complete understanding of the signals required for progenitor cell growth...
January 17, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28059714/regeneration-of-fat-cells-from-myofibroblasts-during-wound-healing
#6
Maksim V Plikus, Christian F Guerrero-Juarez, Mayumi Ito, Yun Rose Li, Priya H Dedhia, Ying Zheng, Mengle Shao, Denise L Gay, Raul Ramos, Tsai-Ching Hsi, Ji Won Oh, Xiaojie Wang, Amanda Ramirez, Sara E Konopelski, Arijh Elzein, Anne Wang, Rarinthip June Supapannachart, Hye-Lim Lee, Chae Ho Lim, Arben Nace, Amy Guo, Elsa Treffeisen, Thomas Andl, Ricardo N Ramirez, Rabi Murad, Stefan Offermanns, Daniel Metzger, Pierre Chambon, Alan D Widgerow, Tai-Lan Tuan, Ali Mortazavi, Rana K Gupta, Bruce A Hamilton, Sarah E Millar, Patrick Seale, Warren S Pear, Mitchell A Lazar, George Cotsarelis
Although regeneration through the reprogramming of one cell lineage to another occurs in fish and amphibians, it has not been observed in mammals. We discovered in the mouse that during wound healing, adipocytes regenerate from myofibroblasts, a cell type thought to be differentiated and nonadipogenic. Myofibroblast reprogramming required neogenic hair follicles, which triggered bone morphogenetic protein (BMP) signaling and then activation of adipocyte transcription factors expressed during development. Overexpression of the BMP antagonist Noggin in hair follicles or deletion of the BMP receptor in myofibroblasts prevented adipocyte formation...
February 17, 2017: Science
https://www.readbyqxmd.com/read/27997570/the-study-on-biological-function-of-keratin-26-a-novel-member-of-liaoning-cashmere-goat-keratin-gene-family
#7
Mei Jin, Jing Wang, Ming-Xing Chu, Jun Piao, Jing-Ai Piao, Feng-Qin Zhao
In our research, we explored the relationship between Keratin 26 and the regulation of fine hair, BMP signaling pathway, MT, FGF5, and IGF-I. The result of hybridization in situ revealed that Keratin 26 was specially expressed in cortex of skin hair follicles; the result of immunohistochemistry indicated that Keratin 26 was expressed in internal root sheath, external root sheath. Then, Real-time quantitative PCR results showed that relative expressive quantity of Keratin 26 was 1.08 or 3.3 × greater in secondary follicle than primary follicle during anagen or catagen; the difference during anagen was not remarkable (p>0...
2016: PloS One
https://www.readbyqxmd.com/read/27992514/induction-of-skin-derived-precursor-cells-from-human-induced-pluripotent-stem-cells
#8
Yoriko Sugiyama-Nakagiri, Tsutomu Fujimura, Shigeru Moriwaki
The generation of full thickness human skin from dissociated cells is an attractive approach not only for treating skin diseases, but also for treating many systemic disorders. However, it is currently not possible to obtain an unlimited number of skin dermal cells. The goal of this study was to develop a procedure to produce skin dermal stem cells from induced pluripotent stem cells (iPSCs). Skin-derived precursor cells (SKPs) were isolated as adult dermal precursors that could differentiate into both neural and mesodermal progenies and could reconstitute the dermis...
2016: PloS One
https://www.readbyqxmd.com/read/27983534/osteoblastic-differentiation-and-cell-calcification-of-adamantinomatous-craniopharyngioma-induced-by-bone-morphogenetic-protein-2
#9
Xiaorong Yan, Dezhi Kang, Jun Pan, Changzhen Jiang, Yuanxiang Lin, Songtao Qi
BACKGROUND: The calcification of adamantinomatous craniopharyngioma (ACP) often creates difficulties for surgical therapy. Nevertheless, the mechanism of ACP calcification is unclear. Our previous studies demonstrated that osteoblastic factors might play important roles in ACP calcification. OBJECTIVE: We examined the effects of recombinant human Bmp2 on ACP cell differentiation by testing osteoblastic proteins and calcium deposition. METHODS: The expression of osteoblastic factors including osteopontin (OPN), Runx2, and osterix in Bmp2-treated ACP cells was examined by western blot and/or real time PCR...
December 9, 2016: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/27935820/s113r-mutation-in-slc33a1-leads-to-neurodegeneration-and-augmented-bmp-signaling-in-a-mouse-model
#10
Pingting Liu, Baichun Jiang, Jian Ma, Pengfei Lin, Yinshuai Zhang, Changshun Shao, Wenjie Sun, Yaoqin Gong
The S113R mutation (c.339T>G) (MIM #603690.0001) in SLC33A1 (MIM #603690), an ER membrane acetyl-CoA transporter, has been previously identified in individuals with hereditary spastic paraplegia type 42 (SPG42; MIM #612539). SLC33A1 has also been shown to inhibit the bone morphogenetic protein (BMP) signaling pathway in zebrafish. To better understand the function of SLC33A1, we generated and characterized Slc33a1(S113R) knock-in mice. Homozygous Slc33a1(S113R) mutant mice were embryonic lethal, whereas heterozygous Slc33a1 mutant mice (Slc33a1(wt/mut)) exhibited behavioral abnormalities and central neurodegeneration, which is consistent with hereditary spastic paraplegia (HSP) phenotypes...
January 1, 2017: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/27932058/tnap-stimulates-vascular-smooth-muscle-cell-trans-differentiation-into-chondrocytes-through-calcium-deposition-and-bmp-2-activation-possible-implication-in-atherosclerotic-plaque-stability
#11
Maya Fakhry, Monika Roszkowska, Anne Briolay, Carole Bougault, Alain Guignandon, Juan Ignacio Diaz-Hernandez, Miguel Diaz-Hernandez, Slawomir Pikula, René Buchet, Eva Hamade, Bassam Badran, Laurence Bessueille, David Magne
Atherosclerotic plaque calcification varies from early, diffuse microcalcifications to a bone-like tissue formed by endochondral ossification. Recently, a paradigm has emerged suggesting that if the bone metaplasia stabilizes the plaques, microcalcifications are harmful. Tissue-nonspecific alkaline phosphatase (TNAP), an ectoenzyme necessary for mineralization by its ability to hydrolyze inorganic pyrophosphate (PPi), is stimulated by inflammation in vascular smooth muscle cells (VSMCs). Our objective was to determine the role of TNAP in trans-differentiation of VSMCs and calcification...
December 6, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27930933/calcitonin-induces-collagen-synthesis-and-osteoblastic-differentiation-in-human-periodontal-ligament-fibroblasts
#12
Yibo Wei, Qing Ye, Zhen Tang, Gang Tian, Qiang Zhu, Haocheng Gao, Dalin Wang, Zhizhong Cao
BACKGROUND: Extracellular matrix (ECM) secretion and osteogenic differentiation in periodontal ligament fibroblasts (PDLF) facilitate the neogenesis of alveolar bone, which is the cellular basis for alveolar bone repair. Calcitonin (CT) has been reported to play an important role in promoting ECM expression and inducing osteogenic differentiation in osteoblast, but its effects on PDLFs remain obscure. METHODS: The expression of CT, transforming growth factor-beta 1(TGF-β1) and bone morphogenetic protein (BMP) in gingival crevicular fluid (GCF) was measured by ELISA...
February 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/27888757/bmp4-id2-signaling-pathway-is-a-novel-therapeutic-target-for-late-outgrowth-endothelial-progenitor-cell-mediated-endothelial-injury-repair
#13
Wen-Hao Xia, Long Chen, Jian-Wen Liang, Xiao-Yu Zhang, Chen Su, Xinzhu Tong, Jiang He, Yan Li, Zheng Cao, Xiu-Fang Lin, Jun Tao
BACKGROUND: Endothelial progenitor cells (EPCs) play a pivotal role in endothelial repair following artery injury, however, the molecular mechanism of late outgrowth EPCs (LEPCs) in endothelial repair remained to be studied. Bone morphogenetic protein 4 (BMP4) is involved in vascular injury-mediated mobilization and homing of LEPCs. Here, we investigated the influence of BMP4-modified signaling pathway in LEPC-related endothelial repair of human and underlying molecular mechanism. METHODS AND RESULTS: In vitro, after a 28day culture, human LEPCs were pretreated with different concentrations of recombinant BMP4 (0, 10, 50, or 100ng/mL), which markedly augmented the migration and adhesion in vitro and demonstrated a significantly accelerated in vivo endothelial repair capacity of human LEPCs after transplantation into nude mice with carotid artery denudation injury...
February 1, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/27864336/bmp4-promotes-mouse-ips-cell-differentiation-to-male-germ-cells-via-smad1-5-gata4-id1-and-id2
#14
Shi Yang, Qingqing Yuan, Minghui Niu, Jingmei Hou, Zijue Zhu, Min Sun, Zheng Li, Zuping He
Generation of male germ cells from pluripotent cells could provide male gametes for treating male infertility and offer an ideal model for unveiling molecular mechanisms of spermatogenesis. However, the influence and exact molecular mechanisms, especially downstream effectors of BMP4 signaling pathways, in male germ cell differentiation of the induce pluripotent stem (iPS) cells, remain unknown. This study was designed to explore the role and mechanism of BMP4 signaling in the differentiation of mouse iPS cells to male germ cells...
February 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27843458/expression-of-bmp-and-actin-membrane-bound-inhibitor-is-increased-during-terminal-differentiation-of-mscs
#15
Christian G Pfeifer, Alexandra Karl, Arne Berner, Johannes Zellner, Paul Schmitz, Markus Loibl, Matthias Koch, Peter Angele, Michael Nerlich, Michael B Mueller
Chondrogenic differentiating mesenchymal stem cells (MSCs) are mimicking embryonal endochondral ossification and become hypertrophic. BMP (bone morphogenetic protein) and Activin Membrane Bound Inhibitor (BAMBI) is a pseudoreceptor that regulates the activity of transforming growth factor-β (TGF-β) and BMP signalling during chondrogenesis. Both TGF-β and BMP signalling are regulators of chondrogenic cell differentiation. Human bone marrow derived MSCs were chondrogenically predifferentiated in aggregate culture for 14 days...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27812534/data-on-micrornas-and-microrna-targeted-mrnas-in-xenopus-ectoderm
#16
Vrutant V Shah, Benjamin Soibam, Ruth A Ritter, Ashley Benham, Jamina Oomen, Amy K Sater
Small RNAs from early neural (i.e., Noggin-expressing, or NOG) and epidermal (expressing a constitutively active BMP4 receptor, CABR) ectoderm in Xenopus laevis were sequenced to identify microRNAs (miRs) expressed in each tissue. Argonaute-associated mRNAs were isolated and sequenced to identify genes that are regulated by microRNAs in these tissues. Interactions between these ectodermal miRs and selected miR-regulated mRNAs were predicted using the PITA algorithm; PITA predictions for over 600 mRNAs are presented...
December 2016: Data in Brief
https://www.readbyqxmd.com/read/27795714/bmp4-cooperates-with-retinoic-acid-to-induce-the-expression-of-differentiation-markers-in-cultured-mouse-spermatogonia
#17
Yongguang Yang, Yanmin Feng, Xue Feng, Shangying Liao, Xiuxia Wang, Haiyun Gan, Lixian Wang, Xiwen Lin, Chunsheng Han
Spermatogenesis is sustained by the proliferation and differentiation of spermatogonial stem cells (SSCs). However, the molecules controlling these processes remain largely unknown. Here, we developed a simplified high concentration serum-containing system for the culture of mouse SSCs. Analysis of SSCs markers and transplantation results revealed that the cultured spermatogonia retained stem cell characteristics after long-term in vitro propagation. Using this culture system, the expression and function of bone morphogenetic protein 4 (BMP4) were explored...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27787775/the-three-dimensional-culture-of-epithelial-organoids-derived-from-embryonic-chicken-intestine
#18
Malgorzata Pierzchalska, Malgorzata Panek, Malgorzata Czyrnek, Maja Grabacka
The intestinal epithelium isolated from chicken embryos in last 3 days of development can be used to establish the 3D culture of intestinal organoids. When fragments of epithelial tissue released by incubation with EGTA (2.5 mM, 2 h) are embedded in Matrigel matrix on cell culture inserts the formation of empty spheres covered by epithelial cells is observed in first 24 h of culture. The growth and survival of organoids are supported by the addition of R-spondin 1, Noggin, and prostaglandin E2 to the culture medium...
October 28, 2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27783097/breast-cancer-cells-and-bone-marrow-mesenchymal-stromal-cells-a-regulated-modulation-of-the-breast-tumor-in-the-context-of-immune-response
#19
Mehdi Najar, Hussein Fayyad-Kazan, Wissam H Faour, Bassam Badran, Fabrice Journe, Laurence Lagneaux
OBJECTIVE: The role of direct cell-cell interactions mediating selective bone metastasis by breast cancer cells (BCCs) niche is still mostly unknown. MATERIALS AND METHODS: Conditioned medium and direct cell-cell contacts experiments were used to investigate the effect of bone marrow-derived mesenchymal stromal cells (MSCs), osteoprogenitor-like cells (MG-63) and osteosarcoma cells (SaOS-2) on luminal-like (MCF-7) and basal-like (MDA-MB-231) BCCs flow cytometry was used to assess the purity of isolated BCCs and osteoblasts...
October 25, 2016: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/27776430/baicalein-promotes-angiogenesis-and-odontoblastic-differentiation-via-the-bmp-and-wnt-pathways-in-human-dental-pulp-cells
#20
Sang-Im Lee, Sun-Young Kim, Kyung-Ran Park, Eun-Cheol Kim
Baicalein is an active flavonoid extracted from the root of Scutellaria baicalensis that has anticancer and anti-inflammatory properties; its effects on osteoblastic and angiogenic potential are controversial. The aim of this study was to investigate the effects of baicalein on odontoblastic differentiation and angiogenesis and the underlying mechanism in human dental pulp cells (HDPCs). Baicalein (1-10[Formula: see text][Formula: see text]M) had no cytotoxic effects and promoted alkaline phosphatase (ALP) activity, mineralization assayed by Alizarin Red-S staining, and the mRNA expression of marker genes, in a concentration-dependent manner...
2016: American Journal of Chinese Medicine
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