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https://www.readbyqxmd.com/read/29436597/the-interplay-of-bmp4-and-il%C3%A2-7-regulates-the-apoptosis-of-intestinal-intraepithelial-lymphocytes-under-conditions-of-ischemia%C3%AC-reperfusion
#1
Binyu Luo, Kang Chen, Qi Feng, Weidong Xiao, Dan Ma, Hua Yang, Chaojun Zhang
The number and function of intestinal intraepithelial lymphocytes (IELs) have been found to be significantly reduced following induction of acute intestinal mucosal damage via intestinal ischemia̸reperfusion (I̸R). However, the mechanism underlying this reduction remains unclear. Therefore, it was hypothesized that the interplay of bone morphogenetic protein (BMP)4 and interleukin (IL)‑7 regulates IEL function and number. Recent studies have demonstrated that the different components of the BMP2̸4 signaling pathway are expressed in intestinal epithelial cells (IECs) via the activation of nuclear factor (NF)‑κB...
February 9, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29407213/iron-released-from-reactive-microglia-by-noggin-improves-myelin-repair-in-the-ischemic-brain
#2
Jin A Shin, Yul A Kim, Hye Won Kim, Hee-Sun Kim, Kyung-Eun Lee, Jihee Lee Kang, Eun-Mi Park
We previously reported that the bone morphogenetic protein (BMP) antagonist, noggin, improved the repair process with an increase in the reactive microglia/macrophage population in the ischemic brain. Since BMP plays a role in intracellular iron homeostasis via the hepcidin/ferroportin axis, and iron is required for myelination, this study was aimed to determine whether noggin affected iron status and remyelination in the brain following ischemic stroke. We further examined the effect of blocking the BMP/hepcidin pathway on reactive microglia (BV2) and myelination of oligodendroglial cells (MO3...
January 30, 2018: Neuropharmacology
https://www.readbyqxmd.com/read/29400080/chronic-intermittent-hypobaric-hypoxia-provides-vascular-protection-in-aorta-of-two-kidney-one-clip-rat-model-of-hypertension
#3
Fu Congrui, Li Na, Yuan Yujia, Wang Ri, Chen Jinting, Yang Jing, Guo Zan, Wang Sheng, Zhang Yi, Liu Yixian, Dong Jinghui
Many studies have demonstrated that chronic intermittent hypobaric hypoxia (CIHH) can reduce blood pressure in spontaneously hypertensive rats and renovascular hypertensive (RVH) rats in which endothelial dysfunction is determined as a critical factor. However, whether CIHH can regulate vasodilation of the aorta in RVH rats remains unknown. The purpose of this study was to investigate the effect of CIHH on impaired relaxation of the aorta in the two-kidney one-clip (2K1C) RVH rat model. The results showed CIHH improved the impaired endothelium-dependent relaxation in the 2K1C rat aorta...
February 3, 2018: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/29396910/superior-bone-inducing-potential-of-rhbmp9-compared-to-rhbmp2
#4
Masako Fujioka-Kobayashi, Mustafa Abd El Raouf, Nikola Saulacic, Eizaburo Kobayashi, Yufeng Zhang, Benoit Schaller, Richard J Miron
Recombinant human bone morphogenic protein (rhBMP) 9 has recently been reported to have more osteopromotive potential in vitro when compared to rhBMP2. The aim of the present study was to investigate the bone-inducing potential of rhBMP2 and rhBMP9. We compared rhBMP2, rhBMP7 and rhBMP9 at 5 different concentrations and showed convincingly that rhBMP9 possesses much greater potential for osteoblast differentiation even at 20 times lower concentrations in vitro. We further show that Noggin, an inhibitor for rhBMP2-induced osteogenesis, did not alter rhBMP9-induced osteogenesis...
February 2, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29393426/endoglin-cd105-and-smad4-regulate-spheroid-formation-and-the-suppression-of-the-invasive-ability-of-human-pancreatic-cancer-cells
#5
Eri Kokaji, Akiko Shimomura, Takashi Minamisaka, Takahiko Nakajima, Shigeharu Miwa, Hideki Hatta, Takeshi Nishida, Chieko Kiya, Johji Imura
In this study, we investigated the ability of pancreatic cancer cell lines to form spheroids with the aim of identifying factors involved in cell invasiveness, a property that leads to a poor prognosis in pancreatic cancer. For this purpose, 8 cell lines derived from human pancreatic cancer tissues were cultured in non-adherent culture conditions to form spheroids, as well as normal monolayers. The morphology of the cells was observed and spheroid diameters measured. mRNA expression was compared between cells cultured under both culture conditions...
February 1, 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29355872/a-silencing-mediated-enhancement-of-osteogenic-differentiation-by-supramolecular-ternary-sirna-polyplexes-comprising-biocleavable-cationic-polyrotaxanes-and-anionic-fusogenic-peptides
#6
Takasuke Inada, Atsushi Tamura, Masahiko Terauchi, Satoshi Yamaguchi, Nobuhiko Yui
Gene silencing of noggin by small interfering RNA (siRNA) is a promising approach for the treatment of bone defects, because noggin deactivates bone morphogenetic protein-2 (BMP-2) and suppresses osteogenic differentiation. Here, we demonstrated the silencing of the noggin gene by siRNA polyplexes composed of noggin-targeted siRNA and biocleavable cationic polyrotaxanes (DMAE-SS-PRX). To improve the endosomal escape efficiencies of the DMAE-SS-PRX/siRNA polyplexes, anionic and fusogenic GALA peptides were integrated onto the DMAE-SS-PRX/siRNA polyplexes via simple electrostatic interactions...
January 22, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29324456/targeted-knockdown-of-bone-morphogenetic-protein-signaling-within-neural-progenitors-protects-the-brain-and-improves-motor-function-following-postnatal-hypoxia-ischemia
#7
Robert W Dettman, Derin Birch, Augusta Fernando, John A Kessler, Maria L V Dizon
Hypoxic-ischemic injury (HI) to the neonatal human brain results in myelin loss that, in some children, can manifest as cerebral palsy. Previously, we had found that neuronal overexpression of the bone morphogenic protein (BMP) inhibitor noggin during development increased oligodendroglia and improved motor function in an experimental model of HI utilizing unilateral common carotid artery ligation followed by hypoxia. As BMPs are known to negatively regulate oligodendroglial fate specification of neural stem cells and alter differentiation of committed oligodendroglia, BMP signaling is likely an important mechanism leading to myelin loss...
January 12, 2018: Developmental Neuroscience
https://www.readbyqxmd.com/read/29259041/paeonol-attenuates-lps-induced-endothelial-dysfunction-and-apoptosis-by-inhibiting-bmp4-and-tlr4-signalling-simultaneously-but-independently
#8
Ker Woon Choy, Yeh Siang Lau, Dharmani Murugan, Paul M Vanhoutte, Mohd Rais Mustafa
Inflammatory injury of the endothelium leads to apoptosis and endothelial dysfunction. The current study explored the effect and mechanisms of paeonol in inflammation-induced apoptosis and endothelial dysfunction induced by lipopolysaccharides (LPS). The effects of paeonol on LPS-induced inflammatory injury were assessed by Western blotting, flow cytometry and reactive oxygen species (ROS) measurement in human umbilical vein endothelial cells (HUVECs) and C57BL/6J mice. Vascular reactivity of isolated mouse aortae was examined using wire myographs...
December 19, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29174869/expression-characteristics-of-bmp2-bmpr-ia-and-noggin-in-different-stages-of-hair-follicle-in-yak-skin
#9
Liang-Li Song, Yan Cui, Si-Jiu Yu, Peng-Gang Liu, Jun Liu, Xue Yang, Jun-Feng He, Qian Zhang
Bone morphogenetic protein 2 (BMP2), BMP receptor-IA (BMPR-IA), and the BMP2 antagonist Noggin are important proteins involved in regulating the hair follicle (HF) cycle in skin. In order to explore the expression profiles of BMP2, BMPR-IA, and Noggin in the HF cycle of yak skin, we collected adult yak skin in the telogen, proanagen, and midanagen phases of HFs and evaluated gene and protein expression by real-time quantitative polymerase chain reaction (qRT-PCR), western blotting, and immunohistochemistry...
November 22, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29167826/efficient-in-vitro-delivery-of-noggin-sirna-enhances-osteoblastogenesis
#10
S Ghadakzadeh, R C Hamdy, M Tabrizian
Several types of serious bone defects would not heal without invasive clinical intervention. One approach to such defects is to enhance the capacity of bone-formation cells. Exogenous bone morphogenetic proteins (BMP) have been utilized to positively regulate matrix mineralization and osteoblastogenesis, however, numerous adverse effects are associated with this approach. Noggin, a potent antagonist of BMPs, is an ideal candidate to target and decrease the need for supraphysiological doses of BMPs. In the current research we report a novel siRNA-mediated gene knock-down strategy to down-regulate Noggin...
November 2017: Heliyon
https://www.readbyqxmd.com/read/29130651/further-delineation-of-the-gdf6-related-multiple-synostoses-syndrome
#11
Paulien A Terhal, Nienke E Verbeek, Nine Knoers, Rutger J A J Nievelstein, Ans van den Ouweland, Ralph J Sakkers, Lucienne Speleman, Gijs van Haaften
A mutation in GDF6 was recently found to underlie a multiple synostoses syndrome. In this report, we describe the second family with GDF6-related multiple synostoses syndrome (SYNS4), caused by a novel c.1287C>A/p.Ser429Arg mutation in GDF6. In addition to synostoses of carpal and/or tarsal bones, at least 6 of 10 affected patients in this family have been diagnosed with mild to moderate hearing loss. In four of them otosclerosis was said to be present, one patient had hearing loss due to severe stapes fixation at the age of 6 years, providing evidence that hearing loss in the GDF6-related multiple synostoses syndrome can be present in childhood...
January 2018: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/29115380/combination-treatment-of-adipose-derived-stem-cells-and-adiponectin-attenuates-pulmonary-arterial-hypertension-in-rats-by-inhibiting-pulmonary-arterial-smooth-muscle-cell-proliferation-and-regulating-the-ampk-bmp-smad-pathway
#12
Li Luo, Wuhong Zheng, Guili Lian, Huaning Chen, Ling Li, Changsheng Xu, Liangdi Xie
The present study aimed to assess the effects of therapy with adiponectin (APN) gene-modified adipose-derived stem cells (ADSCs) on pulmonary arterial hypertension (PAH) in rats and the underlying cellular and molecular mechanisms. ADSCs were successfully isolated from the rats and characterized. ADSCs were effectively infected with the green fluorescent protein (GFP)-empty (ADSCs-V) or the APN-GFP (ADSCs-APN) lentivirus and the APN expression was evaluated by ELISA. Sprague-Dawley rats were administered monocrotaline (MCT) to develop PAH...
October 31, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29112887/comparative-performance-analysis-of-human-ipsc-derived-and-primary-neural-progenitor-cells-npc-grown-as-neurospheres-in-vitro
#13
Maxi Hofrichter, Laura Nimtz, Julia Tigges, Yaschar Kabiri, Friederike Schröter, Brigitte Royer-Pokora, Barbara Hildebrandt, Martin Schmuck, Alexey Epanchintsev, Stephan Theiss, James Adjaye, Jean-Marc Egly, Jean Krutmann, Ellen Fritsche
Developmental neurotoxicity (DNT) testing performed in rats is resource-intensive (costs, time, animals) and bears the issue of species extrapolation. Thus, reliable alternative human-based approaches are needed for predicting neurodevelopmental toxicity. Human induced pluripotent stem cells (hiPSCs) represent a basis for an alternative method possibly being part of an alternative DNT testing strategy. Here, we compared two hiPSC neural induction protocols resulting in 3D neurospheres: one using noggin and one cultivating cells in neural induction medium (NIM protocol)...
October 26, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29094227/down-regulation-of-noggin-and-mir-138-coordinately-promote-osteogenesis-of-mesenchymal-stem-cells
#14
Xing-Kun Sun, Jin Zhou, Lei Zhang, Tian Ma, Yu-Han Wang, Yan-Mei Yang, Yan-Ting Tang, Hong Li, Li-Jun Wang
Mesenchymal stem cells (MSCs) can differentiate to osteocytes under suitable conditions. In recent years, micro-nucleotides have been progressively used to modulate gene expression in cells due to the consideration of safety. Our present study aimed to investigate whether co-delivery of Noggin-siRNA and antimiR-138 enhances the osteogenic effect of MSCs. Using a murine MSC line, C3H/10T1/2 cells, the delivery efficiency of Noggin-siRNA and antimiR-138 into MSCs was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR)...
December 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29037671/blue-mussel-mytilus-edulis-protein-hydrolysate-promotes-mouse-mesenchymal-stem-cell-differentiation-into-osteoblasts-through-up-regulation-of-bone-morphogenetic-protein
#15
Jun-Ho Hyung, Chang-Bum Ahn, Jae-Young Je
Seafood provides a range of health benefits due to its high-protein level. In this study, the osteogenic effect of blue mussel (Mytilus edulis) protein hydrolysates (BMPH) on osteoblast differentiation were examined using mouse mesenchymal stem cells (MSCs). A preparation we called BMPH<1kDa which showed the highest osteogenic effect in MSCs, was prepared by peptic hydrolysis. BMPH<1kDa treatment stimulated osteoblast differentiation with alkaline phosphatase (ALP) induction, osteocalcin and type I collagen activity as well as calcium deposition...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28981962/the-bmp-antagonist-noggin-is-produced-by-osteoblasts-in-response-to-the-presence-of-prostate-cancer-cells
#16
Huda F AlShaibi, Farid Ahmed, Clive Buckle, Ann Cm Fowles, Jalaluddin Awlia, Marco G Cecchini, Colby L Eaton
BACKGROUND: Bone metastasis is a key event responsible for morbidity in prostate cancer patients. Interactions between prostate cancer cells and the bone microenvironment facilitate survival of tumour cells and alter bone turnover, a process that is thought to enhance the growth of metastases in this site. This study aimed to test the hypothesis that the presence of tumours cells increases TGFβ signaling in bone and that this regulates the proliferation and differentiation of osteoblastic lineage cells in metastatic sites...
October 5, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28927342/biomimetic-functionalized-surfaces-and-the-induction-of-bone-formation
#17
Ugo Ripamonti
Tissue engineering still needs to assign the molecular basis of pattern formation, tissue induction, and morphogenesis: What next to morphogens and stem cells? Macroporous biomimetic matrices per se, without the addition of the soluble osteogenic molecular signals of the transforming growth factor-β (TGF-β) supergene family, remarkably initiate the induction of bone formation. Carving geometries within different calcium phosphate-based macroporous bioreactors we show that geometric cues imprinted within the macroporous spaces initiate the spontaneous induction of bone...
November 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28903487/from-the-cover-arsenic-induces-hippocampal-neuronal-apoptosis-and-cognitive-impairments-via-an-up-regulated-bmp2-smad-dependent-reduced-bdnf-trkb-signaling-in-rats
#18
Rukmani Pandey, Vipin Rai, Juhi Mishra, Kapil Mandrah, Somendu Kumar Roy, Sanghamitra Bandyopadhyay
Arsenic promotes hippocampal neuronal damage inducing cognitive impairments. However, mechanism arbitrating arsenic-mediated cognitive deficits remains less-known. Here, we identified that chronic exposure to environmentally relevant doses of arsenic increased apoptosis, characterized by caspase-3 activation, poly(ADP-ribose) polymerase cleavage and Terminal deoxynucleotidyl transferase dUTP nick-end labeling of rat hippocampal neurons, marked by NeuN. Investigating apoptotic mechanism through invivo and invitro studies revealed that arsenic promoted bone morphogenetic protein-2 (BMP2) expression, supported by increased BMP-receptor2 (BMPR2) and p-Smad1/5 in hippocampal neurons...
September 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28855280/in-vitro-and-in-vivo-imaging-and-tracking-of-intestinal-organoids-from-human-induced-pluripotent-stem-cells
#19
Kwang Bo Jung, Hana Lee, Ye Seul Son, Ji Hye Lee, Hyun-Soo Cho, Mi-Ok Lee, Jung-Hwa Oh, Jaemin Lee, Seokho Kim, Cho-Rok Jung, Janghwan Kim, Mi-Young Son
Human intestinal organoids (hIOs) derived from human pluripotent stem cells (hPSCs) have immense potential as a source of intestines. Therefore, an efficient system is needed for visualizing the stage of intestinal differentiation and further identifying hIOs derived from hPSCs. Here, 2 fluorescent biosensors were developed based on human-induced pluripotent stem cell (hiPSC) lines that stably expressed fluorescent reporters driven by intestine-specific gene promoters (KLF5(mCherry) and ISX(eGFP)). Then hIOs were efficiently induced from those transgenic hiPSC lines in which monomeric Cherry- or enhanced green fluorescent protein-expressing cells, which appeared during differentiation, could be identified in intact living cells in real time...
August 29, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28846711/identification-of-novel-biomarkers-to-monitor-%C3%AE-cell-function-and-enable-early-detection-of-type-2-diabetes-risk
#20
Kirstine J Belongie, Ele Ferrannini, Kjell Johnson, Patricia Andrade-Gordon, Michael K Hansen, John R Petrie
A decline in β-cell function is a prerequisite for the development of type 2 diabetes, yet the level of β-cell function in individuals at risk of the condition is rarely measured. This is due, in part, to the fact that current methods for assessing β-cell function are inaccurate, prone to error, labor-intensive, or affected by glucose-lowering therapy. The aim of the current study was to identify novel circulating biomarkers to monitor β-cell function and to identify individuals at high risk of developing β-cell dysfunction...
2017: PloS One
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