Seungmi Ryu, Claire Weber, Pei-Hsuan Chu, Ben Ernest, Vukasin M Jovanovic, Tao Deng, Jaroslav Slamecka, Hyenjong Hong, Yogita Jethmalani, Hannah M Baskir, Jason Inman, John Braisted, Marissa B Hirst, Anton Simeonov, Ty C Voss, Carlos A Tristan, Ilyas Singeç
Embryoid bodies (EBs) and self-organizing organoids derived from human pluripotent stem cells (hPSCs) recapitulate tissue development in a dish and hold great promise for disease modeling and drug development. However, current protocols are hampered by cellular stress and apoptosis during cell aggregation, resulting in variability and impaired cell differentiation. Here, we demonstrate that EBs and various organoid models (e.g., brain, gut, and kidney) can be optimized by using the CEPT small molecule cocktail, a polypharmacology approach that ensures cytoprotection and cell survival...
November 16, 2023: Biofabrication