keyword
MENU ▼
Read by QxMD icon Read
search

embryoid body

keyword
https://www.readbyqxmd.com/read/28429716/polyembryoma-of-the-testis-a-report-of-two-cases-dominant-within-mixed-germ-cell-tumors-and-review-of-gonadal-polyembryomas
#1
REVIEW
Jennifer N Stall, Robert H Young
Two testicular mixed germ cell tumors, from men of 21 and 41 years, in which polyembryoma predominated are described. A literature review uncovered an additional five testicular and nine ovarian cases. One tumor occurred in a 60-year-old man, but all others occurred within the typical age range of gonadal germ cell tumors. One male presented with gynecomastia and one female with sexual precocity, but all otherwise had standard clinical manifestations. These tumors are typically large with non-specific gross features, but a few have a prominent hemorrhagic appearance...
April 21, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/28417059/susceptibility-of-human-oral-squamous-cell-carcinoma-oscc-h103-and-h376-cell-lines-to-retroviral-oskm-mediated-reprogramming
#2
Nalini Devi Verusingam, Swee Keong Yeap, Huynh Ky, Ian C Paterson, Suan Phaik Khoo, Soon Keng Cheong, Alan H K Ong, Tunku Kamarul
Although numbers of cancer cell lines have been shown to be successfully reprogrammed into induced pluripotent stem cells (iPSCs), reprogramming Oral Squamous Cell Carcinoma (OSCC) to pluripotency in relation to its cancer cell type and the expression pattern of pluripotent genes under later passage remain unexplored. In our study, we reprogrammed and characterised H103 and H376 oral squamous carcinoma cells using retroviral OSKM mediated method. Reprogrammed cells were characterized for their embryonic stem cells (ESCs) like morphology, pluripotent gene expression via quantitative real-time polymerase chain reaction (RT-qPCR), immunofluorescence staining, embryoid bodies (EB) formation and directed differentiation capacity...
2017: PeerJ
https://www.readbyqxmd.com/read/28403778/myc-decoy-oligodeoxynucleotide-inhibits-growth-and-modulates-differentiation-of-mouse-embryonic-stem-cells-as-a-model-of-cancer-stem-cells
#3
Behrooz Johari, Mina Ebrahimi-Rad, Faezeh Maghsood, Majid Lotfinia, Zohreh Saltanatpouri, Ladan Teimoori-Toolabi, Zahra Sharifzadeh, Morteza Karimipoor, Mehdi Kadivar
Myc (c-Myc) alone activates the embryonic stem cell-like transcriptional module in both normal and transformed cells. Its dysregulation might lead to increased cancer stem cells (CSCs) population in some tumor cells. Because of the technical limitations, difficulties of keeping CSCs in culture and their similarities with mouse embryonic stem cells (mESCs), we used the latter as a model cell of study to analyze the potential of differentiation therapy mediated by Myc decoy approach. To our best of knowledge this is the first report outlining the application of Myc decoy in transcription factor decoy "TFD" strategy for inducing differentiation in mESCs...
April 12, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/28395740/generation-and-characterization-of-two-ipsc-lines-from-human-epicardium-derived-cells
#4
Christina Paulitschek, Peggy Schulze-Matz, Julia Hesse, Timo Schmidt, Wasco Wruck, James Adjaye, Jürgen Schrader
Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid body formation. The iPSC lines and the human embryonic stem cell line H1 show a Pearson correlation co-efficient of 0.951 (MCDU1i-EPDC) and 0.937 (MCDU2i-EPDC) as assessed by comparative transcriptome profiling...
April 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28392218/promyelocytic-leukemia-protein-is-an-essential-regulator-of-stem-cell-pluripotency-and-somatic-cell-reprogramming
#5
Christiana Hadjimichael, Konstantina Chanoumidou, Christoforos Nikolaou, Antonios Klonizakis, Gesthimani-Ioanna Theodosi, Takis Makatounakis, Joseph Papamatheakis, Androniki Kretsovali
Promyelocytic leukemia protein (PML), the main constituent of PML nuclear bodies, regulates various physiological processes in different cell types. However, little is known about its functions in embryonic stem cells (ESC). Here, we report that PML contributes to ESC self-renewal maintenance by controlling cell-cycle progression and sustaining the expression of crucial pluripotency factors. Transcriptomic analysis and gain- or loss-of-function approaches showed that PML-deficient ESC exhibit morphological, metabolic, and growth properties distinct to naive and closer to the primed pluripotent state...
March 30, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28387983/pten-facilitates-epiblast-epithelial-polarization-and-proamniotic-lumen-formation-in-early-mouse-embryos
#6
Yue Meng, Kathy Q Cai, Robert Moore, Wensi Tao, Jeffrey D Tse, Elizabeth R Smith, Xiang-Xi Xu
BACKGROUND: Phosphatase and tensin homologue on chromosome 10 (Pten), a lipid phosphatase originally identified as a tumor-suppressor gene, regulates the phosphoinositol 3 kinase signaling pathway and impacts cell death and proliferation. Pten mutant embryos die at early stages of development, though the particular developmental deficiency and the mechanisms are not yet fully understood. RESULTS: We analyzed Pten mutant embryos in detail and found that the formation of the proamniotic cavity is impaired...
April 7, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28374933/sialylation-is-dispensable-for-early-murine-embryonic-development-in-vitro
#7
Markus Abeln, Kristina Borst, Samanta Cajic, Hauke Thiesler, Elina Kats, Iris Albers, Maike Kuhn, Volkhard Kaever, Erdmann Rapp, Anja Münster-Kühnel, Birgit Weinhold
The negatively charged nonulose sialic acid (Sia) is essential for murine development in vivo. To elucidate the impact of sialylation on differentiation processes in the absence of maternal effects, we generated mouse embryonic stem cell (mESC) lines that lack CMP-Sia synthetase (CMAS) and thereby the capacity of activating Sia to CMP-Sia. Loss of CMAS activity resulted in an asialo cell surface, accompanied by an increase in glycoconjugates with terminal galactosyl and oligo-LacNAc residues as well as intracellular accumulation of free Sia...
April 4, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28373172/mouse-embryonic-dorsal-root-ganglia-contain-pluripotent-stem-cells-that-show-features-similar-to-es-cells-and-ips-cells
#8
Ryuhei Ogawa, Kyohei Fujita, Kazuo Ito
In the present study, we showed that the dorsal root ganglion (DRG) in the mouse embryo contains pluripotent stem cells (PSCs) that have developmental capacities equivalent to those of embryonic stem (ES) cells and induced pluripotent stem cells. Mouse embryonic DRG cells expressed pluripotency-related transcription factors (octamer-binding transcription factor 4, SRY (sex determining region Y)-box containing gene (Sox) 2, and Nanog) that play essential roles in maintaining the pluripotency of ES cells. Furthermore, the DRG cells differentiated into ectoderm-, mesoderm- and endoderm-derived cells...
April 3, 2017: Biology Open
https://www.readbyqxmd.com/read/28370937/the-n-glycome-of-embryonic-stem-cells-and-thereof-differentiated-hepatic-cells
#9
Houda Montacir, Nora Freyer, Fanny Knoespel, Thomas Urbaniak, Tereza Dedova, Markus Berger, Georg Damm, Rudolf Tauber, Katrin Zeilinger, Veronique Blanchard
Human embryonic stem cells (hESC) are unspecialized cells that are used in regenerative medicine. In addition, the have been proposed as an appropriate alternative source of hepatocytes. In this work, hESC were differentiated into definitive endodermal cells (DEC) then hepatocyte-like cells (HLC). Their N-glycome was measured and compared also with the one of primary hepatocytes. hESC contained a high amount of high-mannose N-glycans. In contrast, complex-type N-glycans such as asialylated or monosialylated biantennary and triantennary N-glycans were dominant in HLC and fully-galactosylated structures were much more abundant than in undifferentiated hESC...
March 31, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28361321/formation-of-stomach-tissue-by-organoid-culture-using-mouse-embryonic-stem-cells
#10
Taka-Aki K Noguchi, Akira Kurisaki
In this chapter, we describe a method for the induction of stomach organoids from mouse embryonic stem (ES) cells. We used an embryoid body-based differentiation method to induce gastric primordial epithelium covered with mesenchyme and further differentiate it in Matrigel by 3D culture. The differentiated organoid contains both corpus- and antrum-specific mature gastric tissue cells. This protocol may be useful for a variety of studies in developmental biology and disease modeling of the stomach.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28359056/the-construction-and-identification-of-induced-pluripotent-stem-cells-derived-from-acute-myelogenous-leukemia-cells
#11
Liang-Fang Zhu, Qiu-Ru Chen, Shao-Zhen Chen, Ling-Yan Wang, Xiao-Feng Luo, Jin-Hua Ren, Xiao-Hong Yuan, Xue-Qiong Wu, Yan-Ling Zeng, Min Xiao, Yong-Quan Chen, Ying-Yu Chen, Min-Hui Lin, Zheng-Jun Wu, Zhi-Zhe Chen, Jian-Da Hu, Ting Yang
OBJECTIVE: The present study aimed to establish an induced pluripotent stem cell (iPSC) line from acute myelogenous leukemia (AML) cells in vitro and identify their biological characteristics. METHODS: Cells from the AML-infiltrated skin from an M6 patient were infected with a lentivirus carrying OCT4, SOX2, KLF4 and C-MYC to induce iPSCs. The characteristics of the iPSCs were confirmed by alkaline phosphatase (ALP) staining. The proliferation ability of iPSCs was detected with a CCK-8 assay...
March 29, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28355564/smarcad1-contributes-to-the-regulation-of-naive-pluripotency-by-interacting-with-histone-citrullination
#12
Shu Xiao, Jia Lu, Bharat Sridhar, Xiaoyi Cao, Pengfei Yu, Tianyi Zhao, Chieh-Chun Chen, Darina McDee, Laura Sloofman, Yang Wang, Marcelo Rivas-Astroza, Bhanu Prakash V L Telugu, Dana Levasseur, Kang Zhang, Han Liang, Jing Crystal Zhao, Tetsuya S Tanaka, Gary Stormo, Sheng Zhong
Histone citrullination regulates diverse cellular processes. Here, we report that SMARCAD1 preferentially associates with H3 arginine 26 citrullination (H3R26Cit) peptides present on arrays composed of 384 histone peptides harboring distinct post-transcriptional modifications. Among ten histone modifications assayed by ChIP-seq, H3R26Cit exhibited the most extensive genomewide co-localization with SMARCAD1 binding. Increased Smarcad1 expression correlated with naive pluripotency in pre-implantation embryos...
March 28, 2017: Cell Reports
https://www.readbyqxmd.com/read/28335666/control-of-dental-derived-induced-pluripotent-stem-cells-through-modified-surfaces-for-dental-application
#13
Hyunmin Choi, Kyu-Hyung Park, Ah-Reum Lee, Chin Hee Mun, Yong Dae Shin, Yong-Beom Park, Young-Bum Park
OBJECTIVE: The aim of this study is to investigate the behaviour of iPSc derived from dental stem cells in terms of initial adhesion, differentiation potential on differently surface-treated titanium disc. MATERIALS AND METHODS: iPSc derived from human gingival fibroblasts (hGFs) were established using 4-reprogramming factors transduction with Sendai virus. The hGF-iPSc established in this study exhibited the morphology and growth properties similar to human embryonic stem (ES) cells and expressed pluripotency makers...
March 23, 2017: Acta Odontologica Scandinavica
https://www.readbyqxmd.com/read/28333962/different-regulation-of-limb-development-by-p63-transcript-variants
#14
Manabu Kawata, Yuki Taniguchi, Daisuke Mori, Fumiko Yano, Shinsuke Ohba, Ung-Il Chung, Tomomi Shimogori, Alea A Mills, Sakae Tanaka, Taku Saito
The apical ectodermal ridge (AER), located at the distal end of each limb bud, is a key signaling center which controls outgrowth and patterning of the proximal-distal axis of the limb through secretion of various molecules. Fibroblast growth factors (FGFs), particularly Fgf8 and Fgf4, are representative molecules produced by AER cells, and essential to maintain the AER and cell proliferation in the underlying mesenchyme, meanwhile Jag2-Notch pathway negatively regulates the AER and limb development. p63, a transcription factor of the p53 family, is expressed in the AER and indispensable for limb formation...
2017: PloS One
https://www.readbyqxmd.com/read/28319092/the-tdh-gcn5l1-fbxo15-kbp-axis-limits-mitochondrial-biogenesis-in-mouse-embryonic-stem%C3%A2-cells
#15
Valerio Donato, Massimo Bonora, Daniele Simoneschi, Davide Sartini, Yasusei Kudo, Anita Saraf, Laurence Florens, Michael P Washburn, Matthias Stadtfeld, Paolo Pinton, Michele Pagano
Self-renewing naive mouse embryonic stem cells (mESCs) contain few mitochondria, which increase in number and volume at the onset of differentiation. KBP (encoded by Kif1bp) is an interactor of the mitochondrial-associated kinesin Kif1Bα. We found that TDH, responsible for mitochondrial production of acetyl-CoA in mESCs, and the acetyltransferase GCN5L1 cooperate to acetylate Lys501 in KBP, allowing its recognition by and degradation via Fbxo15, an F-box protein transcriptionally controlled by the pluripotency core factors and repressed following differentiation...
April 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28299522/glycoconjugates-reveal-diversity-of-human-neural-stem-cells-hnscs-derived-from-human-induced-pluripotent-stem-cells-hipscs
#16
Majury Kandasamy, Lars Roll, Daniel Langenstroth, Oliver Brüstle, Andreas Faissner
Neural stem cells (NSCs) have the ability to self-renew and to differentiate into various cell types of the central nervous system. This potential can be recapitulated by human induced pluripotent stem cells (hiPSCs) in vitro. The differentiation capacity of hiPSCs is characterized by several stages with distinct morphologies and the expression of various marker molecules. We used the monoclonal antibodies (mAbs) 487(LeX), 5750(LeX) and 473HD to analyze the expression pattern of particular carbohydrate motifs as potential markers at six differentiation stages of hiPSCs...
March 15, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28298436/chromatin-remodeler-chd4-represses-aberrant-expression-of-tbx3-and-sustains-self-renewal-of-embryonic-stem-cells
#17
Haixin Zhao, Zhijun Han, Xinyuan Liu, Junjie Gu, Fan Tang, Gang Wei, Ying Jin
The unique properties of embryonic stem cells (ESCs), unlimited self-renewal and pluripotent differentiation potential, are sustained by integrated genetic and epigenetic networks composed of a series of transcriptional factors and epigenetic modulators. However, molecular mechanisms underlying the function of these regulators are not fully elucidated. Chd4, an ATPase subunit of the nucleosome remodeling and deacetylase (NuRD) complex, is highly expressed in ESCs. However, its function in the regulation of ESC properties remains elusive...
March 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28288171/the-acceleration-of-cardiomyogenesis-in-embryonic-stem-cells-in-vitro-by-serum-depletion-does-not-increase-the-number-of-developed-cardiomyocytes
#18
Katarzyna Anna Radaszkiewicz, Dominika Sýkorová, Lucia Binó, Jana Kudová, Markéta Bébarová, Jiřina Procházková, Hana Kotasová, Lukáš Kubala, Jiří Pacherník
The differentiation of pluripotent embryonic stem (ES) cells into various lineages in vitro represents an important tool for studying the mechanisms underlying mammalian embryogenesis. It is a key technique in studies evaluating the molecular mechanisms of cardiomyogenesis and heart development and also in embryotoxicology. Herein, modest modifications of the basic protocol for ES cell differentiation into cardiomyocytes were evaluated in order to increase the yield and differentiation status of developed cardiomyocytes...
2017: PloS One
https://www.readbyqxmd.com/read/28266620/two-dimensional-electrophysiological-characterization-of-human-pluripotent-stem-cell-derived-cardiomyocyte-system
#19
Huanqi Zhu, Kelsey S Scharnhorst, Adam Z Stieg, James K Gimzewski, Itsunari Minami, Norio Nakatsuji, Haruko Nakano, Atsushi Nakano
Stem cell-derived cardiomyocytes provide a promising tool for human developmental biology, regenerative therapies, disease modeling, and drug discovery. As human pluripotent stem cell-derived cardiomyocytes remain functionally fetal-type, close monitoring of electrophysiological maturation is critical for their further application to biology and translation. However, to date, electrophysiological analyses of stem cell-derived cardiomyocytes has largely been limited by biologically undefined factors including 3D nature of embryoid body, sera from animals, and the feeder cells isolated from mouse...
March 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28250775/preferential-lineage-specific-differentiation-of-osteoblast-derived-induced-pluripotent-stem-cells-into-osteoprogenitors
#20
Casey L Roberts, Silvia S Chen, Angela C Murchison, Rebecca A Ogle, Michael P Francis, Roy C Ogle, Patrick C Sachs
While induced pluripotent stem cells (iPSCs) hold great clinical promise, one hurdle that remains is the existence of a parental germ-layer memory in reprogrammed cells leading to preferential differentiation fates. While it is problematic for generating cells vastly different from the reprogrammed cells' origins, it could be advantageous for the reliable generation of germ-layer specific cell types for future therapeutic use. Here we use human osteoblast-derived iPSCs (hOB-iPSCs) to generate induced osteoprogenitors (iOPs)...
2017: Stem Cells International
keyword
keyword
67588
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"