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https://www.readbyqxmd.com/read/28527889/enhanced-cardiomyogenic-induction-of-mouse-pluripotent-cells-by-cyclic-mechanical-stretch
#1
Akankshya Shradhanjali, Brandon D Riehl, Jeong Soon Lee, Ligyeom Ha, Jung Yul Lim
The cardiac milieu is mechanically active with spontaneous contraction beginning from early development and persistent through maturation and homeostasis, suggesting that mechanical loading may provide biomimetic myocardial developmental signal. In this study, we tested the role of cyclic mechanical stretch loading in the cardiomyogenesis of pluripotent murine embryonic (P19) stem cells. A Flexcell tension system was utilized to apply equiaxial stretch (12% strain, 1.25 Hz frequency) to P19 cell-derived embryoid bodies (EBs)...
May 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28526652/retinoic-acid-induces-differentiation-of-buffalo-bubalus-bubalis-embryonic-stem-cells-into-germ-cells
#2
Syed Mohmad Shah, Suresh Kumar Singla, Prabhat Palta, Radhey Sham Manik, Manmohan Singh Chauhan
Development of precise and reproducible culture system for in vitro differentiation of embryonic stem (ES) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by limited availability of precursor primordial germ cells (PGCs), but in finally treating the catastrophic infertility. Taking into account the significant role of retinoic acid (RA) during in vivo gametogenesis, we designed the present study to investigate the effects of its stimulation on directing the differentiation of ES cells into germ cells...
May 16, 2017: Gene
https://www.readbyqxmd.com/read/28518115/analysis-of-retinoic-acid-induced-neural-differentiation-of-mouse-embryonic-stem-cells-in-two-and-three-dimensional-embryoid-bodies
#3
Junning Yang, Chuanshen Wu, Ioana Stefanescu, Arie Horowitz
Mouse embryonic stem cells (ESCs) isolated from the inner mass of the blastocyst (typically at day E3.5), can be used as in vitro model system for studying early embryonic development. In the absence of leukemia inhibitory factor (LIF), ESCs differentiate by default into neural precursor cells. They can be amassed into a three dimensional (3D) spherical aggregate termed embryoid body (EB) due to its similarity to the early stage embryo. EBs can be seeded on fibronectin-coated coverslips, where they expand by growing two dimensional (2D) extensions, or implanted in 3D collagen matrices where they continue growing as spheroids, and differentiate into the three germ layers: endodermal, mesodermal, and ectodermal...
April 22, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28518103/structure-function-studies-in-mouse-embryonic-stem-cells-using-recombinase-mediated-cassette-exchange
#4
Tim Pieters, Lieven Haenebalcke, Kenneth Bruneel, Niels Vandamme, Tino Hochepied, Jolanda van Hengel, Dagmar Wirth, Geert Berx, Jody J Haigh, Frans van Roy, Steven Goossens
Gene engineering in mouse embryos or embryonic stem cells (mESCs) allows for the study of the function of a given protein. Proteins are the workhorses of the cell and often consist of multiple functional domains, which can be influenced by posttranslational modifications. The depletion of the entire protein in conditional or constitutive knock-out (KO) mice does not take into account this functional diversity and regulation. An mESC line and a derived mouse model, in which a docking site for FLPe recombination-mediated cassette exchange (RMCE) was inserted within the ROSA26 (R26) locus, was previously reported...
April 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28509913/optimization-of-a-polydopamine-pd-based-coating-method-and-polydimethylsiloxane-pdms-substrates-for-improved-mouse-embryonic-stem-cell-esc-pluripotency-maintenance-and-cardiac-differentiation
#5
Jiayin Fu, Yon Jin Chuah, Wee Tong Ang, Nan Zheng, Dong-An Wang
Myocardiocyte derived from pluripotent stem cells, such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs), is a promising cell source for cardiac tissue engineering. Combined with microfluidic technologies, a heart-on-a-chip is very likely to be developed and function as a platform for high throughput drug screening. Polydimethylsiloxane (PDMS) silicone elastomer is a widely-used biomaterial for the investigation of cell-substrate interactions and biochip fabrication. However, the intrinsic PDMS surface hydrophobicity inhibits cell adhesion on the PDMS surface, and PDMS surface modification is required for effective cell adhesion...
May 16, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28500172/induced-pluripotent-stem-cell-derived-cardiomyocytes-provide-in-vivo-biological-pacemaker-function
#6
Samuel Chauveau, Evgeny P Anyukhovsky, Meital Ben-Ari, Shulamit Naor, Ya-Ping Jiang, Peter Danilo, Tania Rahim, Stephanie Burke, Xiaoliang Qiu, Irina A Potapova, Sergey V Doronin, Peter R Brink, Ofer Binah, Ira S Cohen, Michael R Rosen
BACKGROUND: Although multiple approaches have been used to create biological pacemakers in animal models, induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have not been investigated for this purpose. We now report pacemaker function of iPSC-CMs in a canine model. METHODS AND RESULTS: Embryoid bodies were derived from human keratinocytes, their action potential characteristics determined, and their gene expression profiles and markers of differentiation identified...
May 2017: Circulation. Arrhythmia and Electrophysiology
https://www.readbyqxmd.com/read/28492526/differentiation-of-cardiomyocytes-and-generation-of-human-engineered-heart-tissue
#7
Kaja Breckwoldt, David Letuffe-Brenière, Ingra Mannhardt, Thomas Schulze, Bärbel Ulmer, Tessa Werner, Anika Benzin, Birgit Klampe, Marina C Reinsch, Sandra Laufer, Aya Shibamiya, Maksymilian Prondzynski, Giulia Mearini, Dennis Schade, Sigrid Fuchs, Christiane Neuber, Elisabeth Krämer, Umber Saleem, Mirja L Schulze, Marita L Rodriguez, Thomas Eschenhagen, Arne Hansen
Since the advent of the generation of human induced pluripotent stem cells (hiPSCs), numerous protocols have been developed to differentiate hiPSCs into cardiomyocytes and then subsequently assess their ability to recapitulate the properties of adult human cardiomyocytes. However, hiPSC-derived cardiomyocytes (hiPSC-CMs) are often assessed in single-cell assays. A shortcoming of these assays is the limited ability to characterize the physiological parameters of cardiomyocytes, such as contractile force, due to random orientations...
June 2017: Nature Protocols
https://www.readbyqxmd.com/read/28487523/a-preliminary-analysis-of-volatile-metabolites-of-human-induced-pluripotent-stem-cells-along-the-in-vitro-differentiation
#8
Rosamaria Capuano, Paola Spitalieri, Rosa Valentina Talarico, Ana Carolina Domakoski, Alexandro Catini, Roberto Paolesse, Eugenio Martinelli, Giuseppe Novelli, Federica Sangiuolo, Corrado Di Natale
Cellular metabolism of stem cell biology is still an unexplored field. However, considering the amount of information carried by metabolomes, this is a promising field for a fast identification of stem cells itself and during the differentiation process. One of the goals of such application is the identification of residual pluripotent cells before cell transplantation to avoid the occurrence of teratomas. In this paper, we investigated in vitro the volatile compounds (VOCs) released during human induced pluripotent stem cells (hiPSCs) reprogramming...
May 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28483511/collagen-alginate-microspheres-as-a-3d-culture-system-for-mouse-embryonic-stem-cells-differentiation-to-primordial-germ-cells
#9
Vahid Mansouri, Mohammad Salehi, Mir Davood Omrani, Zahra Niknam, Abdolreza Ardeshirylajimi
Germ cells differentiation of stem cells will aid treatment of adults with infertility. Biopolymers utilization provided synthetic extracellular matrix (ECM) and desired attributes in in vitro to improve conditions for stem cells attachment, proliferation and differentiation. Mixture of alginate as a biocompatible hydrogel, with collagen IV, could establish an in vitro 3 dimensional (3D) culture model. The objective of this study was investigation of the mouse ESCs differentiation capacity to putative primordial germ cells (PGCs) in the alginate and alginate-collagen IV microspheres (CAM)...
May 5, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28480311/strip2-is-indispensable-for-the-onset-of-embryonic-stem-cell-differentiation
#10
Davood Sabour, Sureshkumar Perumal Srinivasan, Susan Rohani, Vilas Wagh, John Antonydas Gaspar, Darius Panek, Mostafa Abootorabi Ardestani, Michael Xavier Doss, Nicole Riet, Hinrich Abken, Jürgen Hescheler, Symeon Papadopoulos, Agapios Sachinidis
The role of striatin interacting protein 2 (Strip2) in differentiation of embryonic stem cells (ESCs) is still under debate. Strip2-silenced murine (KD) ESCs were differentiated for 4, 8, 12, and 16 days. We show that Strip2 is distributed in the perinucleus or nuclei of wild-type (WT) undifferentiated ESCs, but is localized in high-density nuclear bodies in differentiated cells. CellNet analysis of microarray gene expression data for the KD and scrambled control (SCR) embryoid bodies (EBs), as well as immunostainings of key pluripotent factors, demonstrated that differentiation of KD ESCs is repressed...
June 16, 2017: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/28474621/feeder-basic-fibroblast-growth-factor-free-culture-of-human-embryonic-stem-cells-role-of-conditioned-medium-from-immortalized-human-feeders
#11
Pooja Teotia, Shilpa Sharma, Balram Airan, Sujata Mohanty
BACKGROUND & OBJECTIVES: Human embryonic stem cell (hESC) lines are commonly maintained on inactivated feeder cells, in the medium supplemented with basic fibroblast growth factor (bFGF). However, limited availability of feeder cells in culture, and the high cost of growth factors limit their use in scalable expansion of hESC cultures for clinical application. Here, we describe an efficient and cost-effective feeder and bFGF-free culture of hESCs using conditioned medium (CM) from immortalized feeder cells...
December 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/28447755/gene-expression-profile-in-human-induced-pluripotent-stem-cells-chondrogenic-differentiation-in-vitro-part-a
#12
Wiktoria Maria Suchorska, Ewelina Augustyniak, Magdalena Richter, Tomasz Trzeciak
Human induced pluripotent stem cells (hiPSCs) offer promise in regenerative medicine, however more data are required to improve understanding of key aspects of the cell differentiation process, including how specific chondrogenic processes affect the gene expression profile of chondrocyte‑like cells and the relative value of cell differentiation markers. The main aims of the present study were as follows: To determine the gene expression profile of chondrogenic-like cells derived from hiPSCs cultured in mediums conditioned with HC‑402‑05a cells or supplemented with transforming growth factor β3 (TGF‑β3), and to assess the relative utility of the most commonly used chondrogenic markers as indicators of cell differentiation...
May 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28447733/gene-expression-profile-in-human-induced-pluripotent-stem-cells-chondrogenic-differentiation-in%C3%A2-vitro-part-b
#13
Ewelina Augustyniak, Wiktoria Maria Suchorska, Tomasz Trzeciak, Magdalena Richter
The development of human induced pluripotent stem cells (hiPSCs) is considered a turning point in tissue engineering. However, more data are required to improve understanding of key aspects of the cell differentiation process, including how specific chondrogenic processes affect the gene expression profile of chondrocyte‑like cells and the relative value of cell differentiation markers. The main aims of the present study were as follows: To determine the gene expression profile of chondrogenic‑like cells derived from hiPSCs cultured in mediums conditioned with HC‑402‑05a cells or supplemented with transforming growth factor β3 (TGF‑β3), and to assess the relative utility of the most commonly‑used chondrogenic markers as indicators of cell differentiation...
May 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28439945/enhancement-of-cardiomyogenesis-in-murine-stem-cells-by-low-intensity-ultrasound
#14
Ailing Teo, Amir Morshedi, Jen-Chieh Wang, Yufeng Zhou, Mayasari Lim
OBJECTIVES: Low-intensity ultrasound (LIUS) has been shown to enhance bone and cartilage regeneration from stem cells. The ease of its incorporation makes it an attractive mechanical stimulus for not only osteogenesis and chondrogenesis, but also cardiomyogenesis. However, to date, no study has investigated its effects on cardiomyogenesis from embryonic stem cells. METHODS: In this study, murine embryonic stem cells were differentiated via embryoid body formation and plating, and after 3 days they were subjected to daily 10 minutes of LIUS treatment with various conditions: (1) low-pulsed (21 mW/cm(2) , 20% duty cycle), (2) low-continuous, (3) high-pulsed (147 mW/cm(2) , 20% duty cycle), and (4) high-continuous LIUS...
April 25, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28436137/insights-into-in-vitro-spermatogenesis-in-mammals-past-present-future
#15
REVIEW
Amir Fattahi, Zeinab Latifi, Tohid Ghasemnejad, Hamid Reza Nejabati, Mohammad Nouri
Considering the self-renewal and differentiation ability of pluripotent stem cells, some studies have pointed out the possibility of stem cell-derived sperm production. Most studies that test this hypothesis have been conducted on rodents, with some promising results; however, studies on humans are progressing slowly, and have encountered technical and ethical hurdles. Established methods to differentiate stem cells-including embryoid bodies, co-culturing, and various feeder cells-may provide a niche that is similar to in vivo conditions and resolve epigenetic abnormalities, but a gonadal-like three-dimensional structure is still required to produce germ cells with the correct imprinting...
April 24, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28434939/calreticulin-is-required-for-tgf-%C3%AE-induced-epithelial-to-mesenchymal-transition-during-cardiogenesis-in-mouse-embryonic-stem-cells
#16
Fereshteh Karimzadeh, Michal Opas
Calreticulin, a multifunctional endoplasmic reticulum resident protein, is required for TGF-β-induced epithelial-to-mesenchymal transition (EMT) and subsequent cardiomyogenesis. Using embryoid bodies (EBs) derived from calreticulin-null and wild-type (WT) embryonic stem cells (ESCs), we show that expression of EMT and cardiac differentiation markers is induced during differentiation of WT EBs. This induction is inhibited in the absence of calreticulin and can be mimicked by inhibiting TGF-β signaling in WT cells...
May 9, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28429716/polyembryoma-of-the-testis-a-report-of-two-cases-dominant-within-mixed-germ-cell-tumors-and-review-of-gonadal-polyembryomas
#17
REVIEW
Jennifer N Stall, Robert H Young
Two testicular mixed germ cell tumors, from men of 21 and 41 years, in which polyembryoma predominated are described. A literature review uncovered an additional five testicular and nine ovarian cases. One tumor occurred in a 60-year-old man, but all others occurred within the typical age range of gonadal germ cell tumors. One male presented with gynecomastia and one female with sexual precocity, but all otherwise had standard clinical manifestations. These tumors are typically large with non-specific gross features, but a few have a prominent hemorrhagic appearance...
April 21, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/28417059/susceptibility-of-human-oral-squamous-cell-carcinoma-oscc-h103-and-h376-cell-lines-to-retroviral-oskm-mediated-reprogramming
#18
Nalini Devi Verusingam, Swee Keong Yeap, Huynh Ky, Ian C Paterson, Suan Phaik Khoo, Soon Keng Cheong, Alan H K Ong, Tunku Kamarul
Although numbers of cancer cell lines have been shown to be successfully reprogrammed into induced pluripotent stem cells (iPSCs), reprogramming Oral Squamous Cell Carcinoma (OSCC) to pluripotency in relation to its cancer cell type and the expression pattern of pluripotent genes under later passage remain unexplored. In our study, we reprogrammed and characterised H103 and H376 oral squamous carcinoma cells using retroviral OSKM mediated method. Reprogrammed cells were characterized for their embryonic stem cells (ESCs) like morphology, pluripotent gene expression via quantitative real-time polymerase chain reaction (RT-qPCR), immunofluorescence staining, embryoid bodies (EB) formation and directed differentiation capacity...
2017: PeerJ
https://www.readbyqxmd.com/read/28403778/myc-decoy-oligodeoxynucleotide-inhibits-growth-and-modulates-differentiation-of-mouse-embryonic-stem-cells-as-a-model-of-cancer-stem-cells
#19
Behrooz Johari, Mina Ebrahimi-Rad, Faezeh Maghsood, Majid Lotfinia, Zohreh Saltanatpouri, Ladan Teimoori-Toolabi, Zahra Sharifzadeh, Morteza Karimipoor, Mehdi Kadivar
Myc (c-Myc) alone activates the embryonic stem cell-like transcriptional module in both normal and transformed cells. Its dysregulation might lead to increased cancer stem cells (CSCs) population in some tumor cells. Because of the technical limitations, difficulties of keeping CSCs in culture and their similarities with mouse embryonic stem cells (mESCs), we used the latter as a model cell of study to analyze the potential of differentiation therapy mediated by Myc decoy approach. To our best of knowledge this is the first report outlining the application of Myc decoy in transcription factor decoy "TFD" strategy for inducing differentiation in mESCs...
April 12, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/28395740/generation-and-characterization-of-two-ipsc-lines-from-human-epicardium-derived-cells
#20
Christina Paulitschek, Peggy Schulze-Matz, Julia Hesse, Timo Schmidt, Wasco Wruck, James Adjaye, Jürgen Schrader
Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid body formation. The iPSC lines and the human embryonic stem cell line H1 show a Pearson correlation co-efficient of 0.951 (MCDU1i-EPDC) and 0.937 (MCDU2i-EPDC) as assessed by comparative transcriptome profiling...
April 2017: Stem Cell Research
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