keyword
MENU ▼
Read by QxMD icon Read
search

embryoid body

keyword
https://www.readbyqxmd.com/read/28817660/cited4-is-related-to-cardiogenic-induction-and-maintenance-of-proliferation-capacity-of-embryonic-stem-cell-derived-cardiomyocytes-during-in-vitro-cardiogenesis
#1
Junichiro Miake, Tomomi Notsu, Katsumi Higaki, Kyoko Hidaka, Takayuki Morisaki, Kazuhiro Yamamoto, Ichiro Hisatome
Cardiac progenitor cells have a limited proliferative capacity. The CREB-binding protein/p300-interacting transactivator, with the Glu/Asp-rich carboxy-terminal domain (Cited) gene family, regulates gene transcription. Increased expression of the Cited4 gene in an adult mouse is associated with exercise-induced cardiomyocyte hypertrophy and proliferation. However, the expression patterns and functional roles of the Cited4 gene during cardiogenesis are largely unknown. Therefore, in the present study, we investigated the expression patterns and functional roles of the Cited4 gene during in vitro cardiogenesis...
2017: PloS One
https://www.readbyqxmd.com/read/28768794/embryonic-stem-cell-derived-germ-cells-induce-spermatogenesis-following-transplantation-to-testis-of-adult-mouse-azoospermia-model
#2
Zohreh Makoolati, Mansoureh Movahedin, Mehdi Forouzandeh-Moghadam, Majid Naghdi, Morteza Koruji
This study aimed to (a) identify the exogenous factors that allow in vitro differentiation of mouse SSCs from embryonic stem cells (ESCs); (b) evaluate the effects of Sertoli cells on SSCs enrichment; and (c) to assess the success of transplantation using in vitro differentiated SSCs in mouse busulfan-treated azoospermia model. One-day-old embryoid body (EB) received 5 ng/ml BMP4 for 4 days, 3 ┬ÁM retinoic acid (RA) in STO co-culture (Co-C) system for 7 days and subsequently co-cultured for 2 days with Sertoli cell in the presence or absence of LIF, bFGF and RA composition and in the presence of these factors in the simple culture media...
August 2, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28752164/in-situ-generation-of-human-brain-organoids-on-a-micropillar-array
#3
Yujuan Zhu, Li Wang, Hao Yu, Fangchao Yin, Yaqing Wang, Haitao Liu, Lei Jiang, Jianhua Qin
Brain organoids derived from human induced pluripotent stem cells can recapitulate the early stages of brain development, representing a powerful in vitro system for modeling brain development and diseases. However, the existing methods for brain organoid formation often require time-consuming procedures, including the initial formation of embryoid bodies (EBs) from hiPSCs, and subsequent neural induction and differentiation companied by multi-steps of cell transfer and encapsulation in a 3D matrix. Herein, we propose a simple strategy to enable in situ formation of massive brain organoids from hiPSCs on a micropillar array without tedious manual procedures...
July 28, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28751729/high-resolution-temporal-transcriptomics-of-mouse-embryoid-body-development-reveals-complex-expression-dynamics-of-coding-and-noncoding-loci
#4
Brian S Gloss, Bethany Signal, Seth W Cheetham, Franziska Gruhl, Dominik C Kaczorowski, Andrew C Perkins, Marcel E Dinger
Cellular responses to stimuli are rapid and continuous and yet the vast majority of investigations of transcriptional responses during developmental transitions typically use long interval time courses; limiting the available interpretive power. Moreover, such experiments typically focus on protein-coding transcripts, ignoring the important impact of long noncoding RNAs. We therefore evaluated coding and noncoding expression dynamics at unprecedented temporal resolution (6-hourly) in differentiating mouse embryonic stem cells and report new insight into molecular processes and genome organization...
July 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28745632/differentiating-chondrocytes-from-peripheral-blood-derived-human-induced-pluripotent-stem-cells
#5
Yueying Li, Yong Hai, Jiayu Chen, Tie Liu
In this study, we used peripheral blood cells (PBCs) as seed cells to produce chondrocytes via induced pluripotent stem cells (iPSCs) in an integration-free method. Following embryoid body (EB) formation and fibroblastic cell expansion, the iPSCs are induced for chondrogenic differentiation for 21 days under serum-free and xeno-free conditions. After chondrocyte induction, the phenotypes of the cells are evaluated by morphological, immunohistochemical, and biochemical analyses, as well as by the quantitative real-time PCR examination of chondrogenic differentiation markers...
July 18, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28736154/retinoic-acid-induces-differentiation-of-buffalo-bubalus-bubalis-embryonic-stem-cells-into-germ-cells
#6
Syed Mohmad Shah, Suresh Kumar Singla, Prabhat Palta, Radhey Sham Manik, Manmohan Singh Chauhan
Development of precise and reproducible culture system for in vitro differentiation of embryonic stem (ES) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by limited availability of precursor primordial germ cells (PGCs), but in finally treating the catastrophic infertility. Taking into account the significant role of retinoic acid (RA) during in vivo gametogenesis, we designed the present study to investigate the effects of its stimulation on directing the differentiation of ES cells into germ cells...
July 19, 2017: Gene
https://www.readbyqxmd.com/read/28732246/inspiration-from-heart-development-biomimetic-development-of-functional-human-cardiac-organoids
#7
Dylan J Richards, Robert C Coyle, Yu Tan, Jia Jia, Kerri Wong, Katelynn Toomer, Donald R Menick, Ying Mei
Recent progress in human organoids has provided 3D tissue systems to model human development, diseases, as well as develop cell delivery systems for regenerative therapies. While direct differentiation of human embryoid bodies holds great promise for cardiac organoid production, intramyocardial cell organization during heart development provides biological foundation to fabricate human cardiac organoids with defined cell types. Inspired by the intramyocardial organization events in coronary vasculogenesis, where a diverse, yet defined, mixture of cardiac cell types self-organizes into functional myocardium in the absence of blood flow, we have developed a defined method to produce scaffold-free human cardiac organoids that structurally and functionally resembled the lumenized vascular network in the developing myocardium, supported hiPSC-CM development and possessed fundamental cardiac tissue-level functions...
October 2017: Biomaterials
https://www.readbyqxmd.com/read/28726548/wnt-yap-interactions-during-neural-tissue-patterning-of-human-induced-pluripotent-stem-cells
#8
Julie Bejoy, Liqing Song, Yi Zhou, Yan Li
Human induced pluripotent stem cells (hiPSCs) have special ability to self-assemble into neural spheroids or mini-brain like structures. During the self-assembly process, Wnt signaling plays an important role in regional patterning and establishing positional identity of hiPSC-derived neural progenitors. Recently, the role of Wnt signaling in regulating Yes-associated protein (YAP) expression (nuclear or cytoplasmic), the pivotal regulator during organ growth and tissue generation, has attracted increasing interests...
July 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28720304/characterization-of-the-single-cell-derived-bovine-induced-pluripotent-stem-cells
#9
Lixia Zhao, Zixin Wang, Jindun Zhang, Jian Yang, Xuefei Gao, Baojiang Wu, Gaoping Zhao, Siqin Bao, Shuxiang Hu, Pentao Liu, Xihe Li
Single-cell derived bovine induced pluripotent stem cells (iPSCs) were generated by the introduction of piggyBac transposons with CAG promoting transcription factors (Oct3/4, Sox2, Klf4 and cMyc). In the study, the bovine iPSCs colony from single cell could passage more than 50 passages after enzymatic dissociation into single cells. These bovine iPSCs cells kept the normal karyotype and displayed dome shaped clones similar to mouse embryonic stem cells. They showed pluripotency in many ways, including their expression of pluripotency markers, such as OCT3/4, NANOG, SOX2, SSEA1, SSEA4, and AP in immunofluorescence assay, Oct4, Nanog, Sox2, Klf4 and cMyc in RT-PCR...
May 22, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28716123/differentiating-mouse-embryonic-stem-cells-express-markers-of-human-endometrium
#10
P Parasar, C R Sacha, N Ng, E R McGuirk, S Chinthala, P Ozcan, J Lindsey, S Salas, M R Laufer, S A Missmer, R M Anchan
BACKGROUND: Modeling early endometrial differentiation is a crucial step towards understanding the divergent pathways between normal and ectopic endometrial development as seen in endometriosis. METHODS: To investigate these pathways, mouse embryonic stem cells (mESCs) and embryoid bodies (EBs) were differentiated in standard EB medium (EBM). Immunofluorescence (IF) staining and reverse-transcription polymerase chain reaction (RT-PCR) were used to detect expression of human endometrial cell markers on differentiating cells, which were sorted into distinct populations using fluorescence-activated cell sorting (FACS)...
July 17, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28689068/efficient-induction-of-inner-ear-hair-cell-like-cells-from-mouse-es-cells-using-combination-of-math1-transfection-and-conditioned-medium-from-st2-stromal-cells
#11
Yukiteru Ouji, Masaharu Sakagami, Hiroko Omori, Shinji Higashiyama, Norikazu Kawai, Tadashi Kitahara, Akio Wanaka, Masahide Yoshikawa
We sought to establish a more efficient technique for induction of inner ear hair cell-like cells (HC-like cells) from embryonic stem cells (ES cells) by using a combination of two previously reported methods; ST2 stromal cell-conditioned medium, known to be favorable for HC-like cell induction (HIST2 method), and ES cells with transfer of the Math1 gene (Math1-ES cells). Math1-ES cells carrying Tet-inducible Math1 were cultured for 14days with doxycycline in conditioned medium from cultures of ST2 stromal cells following formation of 4-day embryoid bodies (EBs)...
July 1, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28683563/modulation-of-hematopoietic-lineage-specification-impacts-trem2-expression-in-microglia-like-cells-derived-from-human-stem-cells
#12
Peter J Amos, Susan Fung, Amanda Case, Jerusalem Kifelew, Leah Osnis, Carole L Smith, Kevin Green, Alipi Naydenov, Macarena Aloi, Jesse J Hubbard, Aravind Ramakrishnan, Gwenn A Garden, Suman Jayadev
Microglia are the primary innate immune cell type in the brain, and their dysfunction has been linked to a variety of central nervous system disorders. Human microglia are extraordinarily difficult to obtain for experimental investigation, limiting our ability to study the impact of human genetic variants on microglia functions. Previous studies have reported that microglia-like cells can be derived from human monocytes or pluripotent stem cells. Here, we describe a reproducible relatively simple method for generating microglia-like cells by first deriving embryoid body mesoderm followed by exposure to microglia relevant cytokines...
July 2017: ASN Neuro
https://www.readbyqxmd.com/read/28674807/an-efficient-transfection-method-for-differentiation-and-cell-proliferation-of-mouse-embryonic-stem-cells
#13
Jun-Yang Liou, Bor-Sheng Ko, Tzu-Ching Chang
Embryonic stem (ES) cells are an important source of stem cells in tissue engineering and regenerative medicine because of their high self-renewal capacities and differentiation potentials. However, the detailed molecular mechanisms controlling the differentiation and renewal programs in ES cells remained unclear. One of the difficulties in understanding these mechanisms substantially results from the low efficacies of gene manipulation by delivering exogenous gene expression or knockdown of endogenous gene expression with small interfering RNA (siRNA) in ES cells...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28673813/advanced-developmental-toxicity-test-method-based-on-embryoid-body-s-area
#14
Hee Young Kang, Young-Kwon Choi, Na Rae Jo, Jae-Hwan Lee, Changhwan Ahn, Il Young Ahn, Tae Sung Kim, Ki-Suk Kim, Kyung-Chul Choi, Jong Kwon Lee, Sung Duk Lee, Eui-Bae Jeung
Embryonic stem cell test (EST) evaluates the embryotoxic potential of substances and measures the half inhibition in viability of mouse embryonic stem cells (ESCs), fibroblasts (3T3 cells) and in cardiac differentiation of ESC. In this study, we suggest the developmental toxicity test method (termed EBT) applying area of embryoid bodies (EBs) instead of cardiac differentiation of EST. In the assessment of 21 substances, EB area was logarithmically decreased in dose-dependent manner. Decline in EB area resulted in decrease of beating ratio during differentiation of ESCs...
June 30, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28673682/efficient-neural-differentiation-of-mouse-pluripotent-stem-cells-in-a-serum-free-medium-and-development-of-a-novel-strategy-for-enrichment-of-neural-cells
#15
Isha Verma, Zubin Rashid, Sujit K Sikdar, Polani B Seshagiri
Pluripotent stem cells (PSCs) offer an excellent model to study neural development and function. Although various protocols have been developed to direct the differentiation of PSCs into desired neural cell types, many of them suffer from limitations including low efficiency, long duration of culture, and the use of expensive, labile, and undefined growth supplements. In this study, we achieved efficient differentiation of mouse PSCs to neural lineage, in the absence of exogenous molecules, by employing a serum-free culture medium containing knockout serum replacement (KSR)...
July 1, 2017: International Journal of Developmental Neuroscience
https://www.readbyqxmd.com/read/28672920/in-vivo-repair-of-full-thickness-cartilage-defect-with-human-ipsc-derived-mesenchymal-progenitor-cells-in-a-rabbit-model
#16
Xingquan Xu, Dongquan Shi, Yubao Liu, Yao Yao, Jin Dai, Zhihong Xu, Dongyang Chen, Huajian Teng, Qing Jiang
Cell-based tissue engineering has the potential to restore cartilage defects. Induced pluripotent stem cells (iPSCs) are regarded as an alternative cell source in regenerative medicine. The purpose of the present study was to evaluate the use of mesenchymal stem cells (MSCs) derived from human iPSCs (hiPSCs) for the regeneration of cartilage defects in a rabbit model. Cartilage defects were made in the patellar grooves of New Zealand white rabbits. The rabbits were then divided into three groups according to implantation: Control group, scaffold implantation group and scaffold/hiPSCs-MSCs (experimental) group...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28672915/induction-of-reprogramming-of-human-amniotic-epithelial-cells-into-ips-cells-by-overexpression-of-yap-oct4-and-sox2-through-the-activation-of-the-hippo-yap-pathway
#17
Yanhui Zhao, Xinfeng Fei, Jianming Guo, Gang Zou, Weidong Pan, Jingju Zhang, Yongyi Huang, Te Liu, Weiwei Cheng
The present study has reported a novel method for producing induced pluripotent stem (iPS) cells. Primary human amniotic epithelial cells (HuAECs) were isolated from the amniotic membranes of pregnant women who received Cesarean sections. These cells were infected with retroviruses carrying octamer-binding transcription factor 4 (Oct4), (sex determining region Y)-box 2 (Sox2) and Yes-associated protein (Yap) (OSY). Following in vitro culture for ~14 days, epithelial-like HuAECs exhibited several iPS clone-like cell colonies (OSY-iPS)...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28671202/transcriptome-dynamics-of-human-pluripotent-stem-cell-derived-contracting-cardiomyocytes-using-an-embryoid-body-model-with-fetal-bovine-serum
#18
Kwang Bo Jung, Ye Seul Son, Hana Lee, Cho-Rok Jung, Janghwan Kim, Mi-Young Son
Cardiomyocyte (CM) differentiation techniques for generating adult-like mature CMs remain imperfect, and the plausible underlying mechanisms remain unclear; however, there are a number of current protocols available. Here, to explore the mechanisms controlling cardiac differentiation, we analyzed the genome-wide transcription dynamics occurring during the differentiation of human pluripotent stem cells (hPSCs) into CMs using embryoid body (EB) formation. We optimized and updated the protocol to efficiently generate contracting CMs from hPSCs by adding fetal bovine serum (FBS) as a medium supplement, which could have a significant impact on the efficiency of cardiac differentiation...
July 25, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28669362/putative-germline-and-pluripotent-stem-cells-in-adult-mouse-ovary-and-their-in-vitro-differentiation-potential-into-oocyte-like-and-somatic-cells
#19
Yashar Esmaeilian, Arzu Atalay, Esra Erdemli
According to classical knowledge of reproductive biology, in the ovary of female mammals there is a limited number of oocytes and there is no possibility of renewal if the oocytes are lost due to disease or injury. However, in recent years, the results of some studies on renewal and formation of oocytes and follicles in the adult mammalian ovary have led to the questioning of this opinion. The aim of our study is to demonstrate the presence of putative germline and pluripotent stem cells in the adult mouse ovary and their differentiation potential into germ and somatic cells...
June 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28666888/antofine-a-natural-phenanthroindolizidine-alkaloid-suppresses-angiogenesis-via-regulation-of-akt-mtor-and-ampk-pathway-in-endothelial-cells-and-endothelial-progenitor-cells-derived-from-mouse-embryonic-stem-cells
#20
Jedo Oh, Gi Dae Kim, Sanghee Kim, Sang Kook Lee
Although antofine, a natural phenanthroindolizidine alkaloid, exerts potential biological activities, including anticancer effect and anti-angiogenic activity, the underlying mechanisms have not yet been investigated. In the present study, the inhibitory effect of antofine on angiogenesis was determined in cultured mouse embryonic stem (mES)/embryoid body (EB)-derived endothelial cells and vascular endothelial growth factor (VEGF)-induced human umbilical vein endothelial cells (HUVECs). Antofine effectively inhibited VEGF-induced cell migration and tube formation of HUVECs...
September 2017: Food and Chemical Toxicology
keyword
keyword
67588
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"