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https://www.readbyqxmd.com/read/28921950/highly-efficient-and-rapid-neural-differentiation-of-mouse-embryonic-stem-cells-based-on-retinoic-acid-encapsulated-porous-nanoparticle
#1
Se-Jin Park, Seongchan Kim, Sung-Yon Kim, Noo Li Jeon, Joon Myong Song, Cheolhee Won, Dal-Hee Min
An improved cell conversion strategy for neural differentiation of mouse embryonic stem (mES) cells is developed by incorporating functionalized mesoporous silica nanoparticle (MSN) as an efficient delivery carrier of retinoic acid (RA), which is pleiotropic factor required for initiation of neural differentiation. Traditional RA-mediated neural differentiation methods required either pre-activation of the cells to the differentiating state by embryoid body (EB) formation or repetitive treatment of the differentiation factor...
September 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28904534/three-dimensional-organoid-system-transplantation-technologies-in-future-treatment-of-central-nervous-system-diseases
#2
REVIEW
NaiLi Wei, ZiFang Quan, Hailiang Tang, JianHong Zhu
In recent years, scientists have made great achievements in understanding the development of human brain and elucidating critical elements of stepwise spatiotemporal control strategies in neural stem cell specification lineage, which facilitates successful induction of neural organoid in vitro including the cerebral cortex, cerebellar, neural tube, hippocampus cortex, pituitary, and optic cup. Besides, emerging researches on neural organogenesis promote the application of 3D organoid system transplantation in treating central nervous system (CNS) diseases...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28904317/notch-signaling-partly-regulates-the-osteogenic-differentiation-of-retinoic-acid-treated-murine-induced-pluripotent-stem-cells
#3
Thanaphum Osathanon, Jeeranan Manokawinchoke, Hiroshi Egusa, Prasit Pavasant
Notch signaling is involved in osteogenic differentiation; however, its role differs depending on cell type and differentiation stage. Here, we investigated the involvement of Notch signaling in the osteogenic differentiation of retinoic acid-treated embryoid bodies derived from mouse gingival fibroblast-derived induced pluripotent stem cells (mGF-iPSCs). When cultured in osteogenic media, mGF-iPSCs showed an increase in their expression of osteogenic marker genes and deposited a mineralized matrix. Furthermore, increased levels of mRNA for Notch1, Notch2, and Hey1 were observed...
2017: Journal of Oral Science
https://www.readbyqxmd.com/read/28900152/a-3d-magnetic-tissue-stretcher-for-remote-mechanical-control-of-embryonic-stem-cell-differentiation
#4
Vicard Du, Nathalie Luciani, Sophie Richard, Gaëtan Mary, Cyprien Gay, François Mazuel, Myriam Reffay, Philippe Menasché, Onnik Agbulut, Claire Wilhelm
The ability to create a 3D tissue structure from individual cells and then to stimulate it at will is a major goal for both the biophysics and regenerative medicine communities. Here we show an integrated set of magnetic techniques that meet this challenge using embryonic stem cells (ESCs). We assessed the impact of magnetic nanoparticles internalization on ESCs viability, proliferation, pluripotency and differentiation profiles. We developed magnetic attractors capable of aggregating the cells remotely into a 3D embryoid body...
September 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/28895148/critical-roles-of-hmagea2-in-induced-pluripotent-stem-cell-pluripotency-proliferation-and-differentiation
#5
Song Park, Yonghun Sung, Jain Jeong, Minjee Choi, Jinhee Lee, Wookbong Kwon, Soyoung Jang, Si Jun Park, Jae Young Kim, Sung Hyun Kim, Duhak Yoon, Zae Young Ryoo, Myoung Ok Kim
Induced pluripotent stem (iPS) cells are important for clinical application and stem cell research. Although human melanoma-associated antigen A2 (hMAGEA2) expression is known to affect differentiation in embryonic stem cells, its specific role in iPS cells remains unclear. To evaluate the function of hMAGEA2 and its characteristics in iPS cells, we produced hMAGEA2-overexpressing iPS cells from hMAGEA2-overexpressing transgenic mice. Although the iPS cells with overexpressed hMAGEA2 did not differ in morphology, their pluripotency, and self-renewal related genes (Nanog, Oct3/4, Sox2, and Stat3), expression level was significantly upregulated...
September 11, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28877475/a-single-tcf-transcription-factor-regardless-of-its-activation-capacity-is-sufficient-for-effective-trilineage-differentiation-of-escs
#6
Steven Moreira, Enio Polena, Victor Gordon, Solen Abdulla, Sujeivan Mahendram, Jiayi Cao, Alexandre Blais, Geoffrey A Wood, Anna Dvorkin-Gheva, Bradley W Doble
Co-expression and cross-regulation of the four TCF/LEFs render their redundant and unique functions ambiguous. Here, we describe quadruple-knockout (QKO) mouse ESCs lacking all full-length TCF/LEFs and cell lines rescued with TCF7 or TCF7L1. QKO cells self-renew, despite gene expression patterns that differ significantly from WT, and display delayed, neurectoderm-biased, embryoid body (EB) differentiation. QKO EBs have no contracting cardiomyocytes and differentiate poorly into mesendoderm but readily generate neuronal cells...
September 5, 2017: Cell Reports
https://www.readbyqxmd.com/read/28855904/immunological-properties-of-murine-parthenogenetic-stem-cell-derived-cardiomyocytes-and-engineered-heart-muscle
#7
Michael Didié, Satish Galla, Vijayakumar Muppala, Ralf Dressel, Wolfram-Hubertus Zimmermann
Pluripotent parthenogenetic stem cells (pSCs) can be derived by pharmacological activation of unfertilized oocytes. Homozygosity of the major histocompatibility complex (MHC) in pSCs makes them an attractive cell source for applications in allogeneic tissue repair. This was recently demonstrated for pSC-based tissue-engineered heart repair. A detailed analysis of immunological properties of pSC-derived cardiomyocytes and engineered heart muscle (EHM) thereof is, however, lacking. The aim of this study was to determine baseline and cytokine-inducible MHC class I and MHC class II as well as programmed death ligand-1 (PDL-1) and co-stimulatory protein (CD40, CD80, CD86) expression in pSC-derived cardiomyocytes and pSC-EHM in vitro and in vivo...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28854867/an-embryonic-and-induced-pluripotent-stem-cell-model-for-ovarian-granulosa-cell-development-and-steroidogenesis
#8
Shane Lipskind, Jennifer S Lindsey, Behzad Gerami-Naini, Jennifer L Eaton, Daniel O'Connell, Adam Kiezun, Joshua W K Ho, Nicholas Ng, Parveen Parasar, Michelle Ng, Michael Nickerson, Utkan Demirci, Richard Maas, Raymond M Anchan
Embryoid bodies (EBs) can serve as a system for evaluating pluripotency, cellular differentiation, and tissue morphogenesis. In this study, we use EBs derived from mouse embryonic stem cells (mESCs) and human amniocyte-derived induced pluripotent stem cells (hAdiPSCs) as a model for ovarian granulosa cell (GC) development and steroidogenic cell commitment. We demonstrated that spontaneously differentiated murine EBs (mEBs) and human EBs (hEBs) displayed ovarian GC markers, such as aromatase (CYP19A1), FOXL2, AMHR2, FSHR, and GJA1...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28852428/compartmentalized-embryoid-body-culture-for-induction-of-spatially-patterned-differentiation
#9
Shohei Kaneda, Jiro Kawada, Hidenori Akutsu, Justin Ichida, Yoshiho Ikeuchi, Teruo Fujii
We developed a compartmentalized culture system of single embryoid bodies (EBs) utilizing a through-hole on a membrane to induce spatially patterned differentiation. An EB derived from mouse pluripotent stem cells was immobilized on the through-hole. By introducing a stem cell maintenance medium and a differentiation medium into upper and lower culture compartments, respectively, a localized differentiated state was achieved only in the lower part of EB, which is exposed to the medium in the lower compartment...
July 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28836401/purinergic-receptor-expression-and-potential-association-with-human-embryonic-stem-cell-derived-oligodendrocyte-progenitor-cell-development
#10
Shirin Kashfi, Maryam Peymani, Kamran Ghaedi, Hossein Baharvand, Mohammad Hossein Nasr-Esfahani, Mohammad Javan
OBJECTIVES: Due to recent progress in production of human embryonic stem cell-derived oligodendrocyte progenitor cells (hESC-OPCs) for ameliorating myelin disease such as multiple sclerosis (MS) and the role of purinergic signaling in OPCs development, we avaluated the profile of purinergic receptors expression during development of OPCs from hESC. MATERIALS AND METHODS: In this experimental study, we used reverse transcription and quantitative polymerase chain reaction (RT-qPCR) to obtain more information about potential roles of purinergic receptors during in vitro production of hESC-OPCs...
October 2017: Cell Journal
https://www.readbyqxmd.com/read/28833847/simultaneous-targeted-inhibition-of-sox2-oct4-transcription-factors-using-decoy-oligodeoxynucleotides-to-repress-stemness-properties-in-mouse-embryonic-stem-cells
#11
Behrooz Johari, Jamil Zargan
Transcriptional master regulators like Sox2 and Oct4, which are expressed in various human tumors, have been shown to cause tumor growth promotion as well as epithelial dysplasia by means of interfering with progenitor cell differentiation. In order to investigate the potential of Sox2-Oct4 transcription factor decoy (TFD) strategy for differentiation therapy, mouse embryonic stem cells (mESCs) were used in this study as a model of cancer stem cells (CSCs). Sox2-Oct4 complex decoy ODNs (cd-ODNs) were designed according to their elements in the promoter region of Sox2 gene...
August 18, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28826352/pcl-pdms-pcl-copolymer-based-microspheres-mediate-cardiovascular-differentiation-from-embryonic-stem-cells
#12
Liqing Song, Mohammad Faisal Ahmed, Yan Li, Julie Bejoy, Changchun Zeng, Yan Li
Poly-ϵ-caprolactone (PCL) based microspheres have received much attention as drug or growth factor delivery carriers and tissue engineering scaffolds due to their biocompatibility, biodegradability, and tunable biophysical properties. In addition, PCL and polydimethylsiloxane (PDMS) can be fabricated into thermo-responsive shape memory polymers for various biomedical applications (e.g., smart sutures and vascular stents). However, the influence of biophysical properties of PCL-PDMS based microspheres on stem cell lineage commitment has not been well understood...
August 21, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28817660/cited4-is-related-to-cardiogenic-induction-and-maintenance-of-proliferation-capacity-of-embryonic-stem-cell-derived-cardiomyocytes-during-in-vitro-cardiogenesis
#13
Junichiro Miake, Tomomi Notsu, Katsumi Higaki, Kyoko Hidaka, Takayuki Morisaki, Kazuhiro Yamamoto, Ichiro Hisatome
Cardiac progenitor cells have a limited proliferative capacity. The CREB-binding protein/p300-interacting transactivator, with the Glu/Asp-rich carboxy-terminal domain (Cited) gene family, regulates gene transcription. Increased expression of the Cited4 gene in an adult mouse is associated with exercise-induced cardiomyocyte hypertrophy and proliferation. However, the expression patterns and functional roles of the Cited4 gene during cardiogenesis are largely unknown. Therefore, in the present study, we investigated the expression patterns and functional roles of the Cited4 gene during in vitro cardiogenesis...
2017: PloS One
https://www.readbyqxmd.com/read/28768794/embryonic-stem-cell-derived-germ-cells-induce-spermatogenesis-following-transplantation-to-testis-of-adult-mouse-azoospermia-model
#14
Zohreh Makoolati, Mansoureh Movahedin, Mehdi Forouzandeh-Moghadam, Majid Naghdi, Morteza Koruji
This study aimed to (a) identify the exogenous factors that allow in vitro differentiation of mouse SSCs from embryonic stem cells (ESCs); (b) evaluate the effects of Sertoli cells on SSCs enrichment; and (c) to assess the success of transplantation using in vitro differentiated SSCs in mouse busulfan-treated azoospermia model. One-day-old embryoid body (EB) received 5 ng/ml BMP4 for 4 days, 3 µM retinoic acid (RA) in STO co-culture (Co-C) system for 7 days and subsequently co-cultured for 2 days with Sertoli cell in the presence or absence of LIF, bFGF and RA composition and in the presence of these factors in the simple culture media...
August 2, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28752164/in-situ-generation-of-human-brain-organoids-on-a-micropillar-array
#15
Yujuan Zhu, Li Wang, Hao Yu, Fangchao Yin, Yaqing Wang, Haitao Liu, Lei Jiang, Jianhua Qin
Brain organoids derived from human induced pluripotent stem cells can recapitulate the early stages of brain development, representing a powerful in vitro system for modeling brain development and diseases. However, the existing methods for brain organoid formation often require time-consuming procedures, including the initial formation of embryoid bodies (EBs) from hiPSCs, and subsequent neural induction and differentiation companied by multi-steps of cell transfer and encapsulation in a 3D matrix. Herein, we propose a simple strategy to enable in situ formation of massive brain organoids from hiPSCs on a micropillar array without tedious manual procedures...
July 28, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28751729/high-resolution-temporal-transcriptomics-of-mouse-embryoid-body-development-reveals-complex-expression-dynamics-of-coding-and-noncoding-loci
#16
Brian S Gloss, Bethany Signal, Seth W Cheetham, Franziska Gruhl, Dominik C Kaczorowski, Andrew C Perkins, Marcel E Dinger
Cellular responses to stimuli are rapid and continuous and yet the vast majority of investigations of transcriptional responses during developmental transitions typically use long interval time courses; limiting the available interpretive power. Moreover, such experiments typically focus on protein-coding transcripts, ignoring the important impact of long noncoding RNAs. We therefore evaluated coding and noncoding expression dynamics at unprecedented temporal resolution (6-hourly) in differentiating mouse embryonic stem cells and report new insight into molecular processes and genome organization...
July 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28745632/differentiating-chondrocytes-from-peripheral-blood-derived-human-induced-pluripotent-stem-cells
#17
Yueying Li, Yong Hai, Jiayu Chen, Tie Liu
In this study, we used peripheral blood cells (PBCs) as seed cells to produce chondrocytes via induced pluripotent stem cells (iPSCs) in an integration-free method. Following embryoid body (EB) formation and fibroblastic cell expansion, the iPSCs are induced for chondrogenic differentiation for 21 days under serum-free and xeno-free conditions. After chondrocyte induction, the phenotypes of the cells are evaluated by morphological, immunohistochemical, and biochemical analyses, as well as by the quantitative real-time PCR examination of chondrogenic differentiation markers...
July 18, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28736154/retinoic-acid-induces-differentiation-of-buffalo-bubalus-bubalis-embryonic-stem-cells-into-germ-cells
#18
Syed Mohmad Shah, Suresh Kumar Singla, Prabhat Palta, Radhey Sham Manik, Manmohan Singh Chauhan
Development of precise and reproducible culture system for in vitro differentiation of embryonic stem (ES) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by limited availability of precursor primordial germ cells (PGCs), but in finally treating the catastrophic infertility. Taking into account the significant role of retinoic acid (RA) during in vivo gametogenesis, we designed the present study to investigate the effects of its stimulation on directing the differentiation of ES cells into germ cells...
August 16, 2017: Gene
https://www.readbyqxmd.com/read/28732246/inspiration-from-heart-development-biomimetic-development-of-functional-human-cardiac-organoids
#19
Dylan J Richards, Robert C Coyle, Yu Tan, Jia Jia, Kerri Wong, Katelynn Toomer, Donald R Menick, Ying Mei
Recent progress in human organoids has provided 3D tissue systems to model human development, diseases, as well as develop cell delivery systems for regenerative therapies. While direct differentiation of human embryoid bodies holds great promise for cardiac organoid production, intramyocardial cell organization during heart development provides biological foundation to fabricate human cardiac organoids with defined cell types. Inspired by the intramyocardial organization events in coronary vasculogenesis, where a diverse, yet defined, mixture of cardiac cell types self-organizes into functional myocardium in the absence of blood flow, we have developed a defined method to produce scaffold-free human cardiac organoids that structurally and functionally resembled the lumenized vascular network in the developing myocardium, supported hiPSC-CM development and possessed fundamental cardiac tissue-level functions...
October 2017: Biomaterials
https://www.readbyqxmd.com/read/28726548/wnt-yes-associated-protein-interactions-during-neural-tissue-patterning-of-human-induced-pluripotent-stem-cells
#20
Julie Bejoy, Liqing Song, Yi Zhou, Yan Li
Human induced pluripotent stem cells (hiPSCs) have special ability to self-assemble into neural spheroids or mini-brain-like structures. During the self-assembly process, Wnt signaling plays an important role in regional patterning and establishing positional identity of hiPSC-derived neural progenitors. Recently, the role of Wnt signaling in regulating Yes-associated protein (YAP) expression (nuclear or cytoplasmic), the pivotal regulator during organ growth and tissue generation, has attracted increasing interests...
August 31, 2017: Tissue Engineering. Part A
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