keyword
MENU ▼
Read by QxMD icon Read
search

betz cell

keyword
https://www.readbyqxmd.com/read/28054585/osseous-differentiation-of-human-fat-tissue-grafts-from-tissue-engineering-to-tissue-differentiation
#1
Maryna Bondarava, Chiara Cattaneo, Bin Ren, Wolfgang E Thasler, Volkmar Jansson, Peter E Müller, Oliver B Betz
Conventional bone tissue engineering approaches require isolation and in vitro propagation of autologous cells, followed by seeding on a variety of scaffolds. Those protracted procedures impede the clinical applications. Here we report the transdifferentiation of human fat tissue fragments retrieved from subcutaneous fat into tissue with bone characteristics in vitro without prior cell isolation and propagation. 3D collagen-I cultures of human fat tissue were cultivated either in growth medium or in osteogenic medium (OM) with or without addition of Bone Morphogenetic Proteins (BMPs) BMP-2, BMP-7 or BMP-9...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28028542/syrosingopine-sensitizes-cancer-cells-to-killing-by-metformin
#2
Don Benjamin, Marco Colombi, Sravanth K Hindupur, Charles Betz, Heidi A Lane, Mahmoud Y M El-Shemerly, Min Lu, Luca Quagliata, Luigi Terracciano, Suzette Moes, Timothy Sharpe, Aleksandra Wodnar-Filipowicz, Christoph Moroni, Michael N Hall
We report that the anticancer activity of the widely used diabetic drug metformin is strongly potentiated by syrosingopine. Synthetic lethality elicited by combining the two drugs is synergistic and specific to transformed cells. This effect is unrelated to syrosingopine's known role as an inhibitor of the vesicular monoamine transporters. Syrosingopine binds to the glycolytic enzyme α-enolase in vitro, and the expression of the γ-enolase isoform correlates with nonresponsiveness to the drug combination. Syrosingopine sensitized cancer cells to metformin and its more potent derivative phenformin far below the individual toxic threshold of each compound...
December 2016: Science Advances
https://www.readbyqxmd.com/read/27982100/spontaneous-isopeptide-bond-formation-as-a-powerful-tool-for-engineering-site-specific-antibody-drug-conjugates
#3
Vanessa Siegmund, Birgit Piater, Bijan Zakeri, Thomas Eichhorn, Frank Fischer, Carl Deutsch, Stefan Becker, Lars Toleikis, Björn Hock, Ulrich A K Betz, Harald Kolmar
Spontaneous isopeptide bond formation, a stabilizing posttranslational modification that can be found in gram-positive bacterial cell surface proteins, has previously been used to develop a peptide-peptide ligation technology that enables the polymerization of tagged-proteins catalyzed by SpyLigase. Here we adapted this technology to establish a novel modular antibody labeling approach which is based on isopeptide bond formation between two recognition peptides, SpyTag and KTag. Our labeling strategy allows the attachment of a reporting cargo of interest to an antibody scaffold by fusing it chemically to KTag, available via semi-automated solid-phase peptide synthesis (SPPS), while equipping the antibody with SpyTag...
December 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27974362/sharpin-regulates-collagen-architecture-and-ductal-outgrowth-in-the-developing-mouse-mammary-gland
#4
Emilia Peuhu, Riina Kaukonen, Martina Lerche, Markku Saari, Camilo Guzmán, Pia Rantakari, Nicola De Franceschi, Anni Wärri, Maria Georgiadou, Guillaume Jacquemet, Elina Mattila, Reetta Virtakoivu, Yuming Liu, Youmna Attieh, Kathleen A Silva, Timo Betz, John P Sundberg, Marko Salmi, Marie-Ange Deugnier, Kevin W Eliceiri, Johanna Ivaska
SHARPIN is a widely expressed multifunctional protein implicated in cancer, inflammation, linear ubiquitination and integrin activity inhibition; however, its contribution to epithelial homeostasis remains poorly understood. Here, we examined the role of SHARPIN in mammary gland development, a process strongly regulated by epithelial-stromal interactions. Mice lacking SHARPIN expression in all cells (Sharpin(cpdm)), and mice with a stromal (S100a4-Cre) deletion of Sharpin, have reduced mammary ductal outgrowth during puberty...
December 14, 2016: EMBO Journal
https://www.readbyqxmd.com/read/27941024/endosomal-phosphatidylinositol-3-phosphate-promotes-gephyrin-clustering-and-gabaergic-neurotransmission-at-inhibitory-postsynapses
#5
Theofilos Papadopoulos, Hong Jun Rhee, Devaraj Subramanian, Foteini Paraskevopoulou, Rainer Mueller, Carsten Schultz, Nils Brose, Jeong-Seop Rhee, Heinrich Betz
The formation of neuronal synapses and the dynamic regulation of their efficacy depends on the proper assembly of the postsynaptic neurotransmitter receptor apparatus. Receptor recruitment to inhibitory GABAergic postsynapses requires the scaffold protein gephyrin and the guanine nucleotide exchange factor collybistin (Cb)2. In vitro, the pleckstrin homology (PH) domain of Cb binds phosphoinositides, specifically phosphatidylinositol-3-phosphate (PI3P). However, whether PI3P is required for inhibitory postsynapse formation is currently unknown...
December 9, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27866708/mutations-in-three-genes-encoding-proteins-involved-in-hair-shaft-formation-cause-uncombable-hair-syndrome
#6
F Buket Ü Basmanav, Laura Cau, Aylar Tafazzoli, Marie-Claire Méchin, Sabrina Wolf, Maria Teresa Romano, Frederic Valentin, Henning Wiegmann, Anne Huchenq, Rima Kandil, Natalie Garcia Bartels, Arzu Kilic, Susannah George, Damian J Ralser, Stefan Bergner, David J P Ferguson, Ana-Maria Oprisoreanu, Maria Wehner, Holger Thiele, Janine Altmüller, Peter Nürnberg, Daniel Swan, Darren Houniet, Aline Büchner, Lisa Weibel, Nicola Wagner, Ramon Grimalt, Anette Bygum, Guy Serre, Ulrike Blume-Peytavi, Eli Sprecher, Susanne Schoch, Vinzenz Oji, Henning Hamm, Paul Farrant, Michel Simon, Regina C Betz
Uncombable hair syndrome (UHS), also known as "spun glass hair syndrome," "pili trianguli et canaliculi," or "cheveux incoiffables" is a rare anomaly of the hair shaft that occurs in children and improves with age. UHS is characterized by dry, frizzy, spangly, and often fair hair that is resistant to being combed flat. Until now, both simplex and familial UHS-affected case subjects with autosomal-dominant as well as -recessive inheritance have been reported. However, none of these case subjects were linked to a molecular genetic cause...
December 1, 2016: American Journal of Human Genetics
https://www.readbyqxmd.com/read/27830219/fluctuations-of-a-membrane-nanotube-revealed-by-high-resolution-force-measurements
#7
F Valentino, P Sens, J Lemière, A Allard, T Betz, C Campillo, C Sykes
Pulling membrane nanotubes from liposomes presents a powerful method to gain access to membrane mechanics. Here we extend classical optical tweezers studies to infer membrane nanotube dynamics with high spatial and temporal resolution. We first validate our force measurement setup by accurately measuring the bending modulus of EPC membrane in tube pulling experiments. Then we record the position signal of a trapped bead when it is connected, or not, to a tube. We derive the fluctuation spectrum of these signals and find that the presence of a membrane nanotube induces higher fluctuations, especially at low frequencies (10-1000 Hz)...
November 10, 2016: Soft Matter
https://www.readbyqxmd.com/read/27790742/the-utility-of-etv1-etv4-and-etv5-rna-in-situ-hybridization-in-the-diagnosis-of-cic-dux-sarcomas
#8
Steven C Smith, Nallasivam Palanisamy, Elizabeth Martin, Jorge Almenara, Jonathan B McHugh, Eun-Young K Choi, David R Lucas, Bryan L Betz, Dafydd Thomas, Rajiv M Patel
AIMS: A recently characterized group of undifferentiated small round cell sarcomas harbours fusions of the genes CIC and DUX4. Studies report a distinctive gene expression profile for these sarcomas, including expression of E26 transformation-specific (ETS) family proto-oncogenic transcription factors ETV1, ETV4 and ETV5. To test the utility of an ancillary diagnostic technique for these tumours, we evaluated chromogenic RNA in-situ hybridization assays for ETV1, ETV4 and ETV5 as diagnostic adjuncts for this emerging group of highly malignant sarcomas...
October 27, 2016: Histopathology
https://www.readbyqxmd.com/read/27758873/systems-analysis-uncovers-inflammatory-th-tc17-driven-modules-during-acute-gvhd-in-monkey-and-human-t-cells
#9
Scott N Furlan, Benjamin Watkins, Victor Tkachev, Sarah Cooley, Angela Panoskaltsis-Mortari, Kayla Betz, Melanie Brown, Daniel J Hunt, John B Schell, Katie Zeleski, Alison Yu, Cynthia R Giver, Edmund K Waller, Jeffrey S Miller, Bruce R Blazar, Leslie S Kean
One of the central challenges of transplantation is the development of alloreactivity despite the use of multiagent immunoprophylaxis. Effective control of this immune suppression-resistant T-cell activation represents one of the key unmet needs in the fields of both solid-organ and hematopoietic stem cell transplant (HCT). To address this unmet need, we have used a highly translational nonhuman primate (NHP) model to interrogate the transcriptional signature of T cells during breakthrough acute graft-versus-host disease (GVHD) that occurs in the setting of clinically relevant immune suppression and compared this to the hyperacute GVHD, which develops in unprophylaxed or suboptimally prophylaxed transplant recipients...
November 24, 2016: Blood
https://www.readbyqxmd.com/read/27757524/pathological-tdp-43-changes-in-betz-cells-differ-from-those-in-bulbar-and-spinal-%C3%AE-motoneurons-in-sporadic-amyotrophic-lateral-sclerosis
#10
Heiko Braak, Albert C Ludolph, Manuela Neumann, John Ravits, Kelly Del Tredici
Two nerve cells types, Betz cells in layer Vb of the primary motor neocortex and α-motoneurons of the lower brainstem and spinal cord, become involved at the beginning of the pathological cascade underlying sporadic amyotrophic lateral sclerosis (sALS). In both neuronal types, the cell nuclei forfeit their normal (non-phosphorylated) expression of the 43-kDa transactive response DNA-binding protein (TDP-43). Here, we present initial evidence that in α-motoneurons the loss of normal nuclear TDP-43 expression is followed by the formation of phosphorylated TDP-43 aggregates (pTDP-43) within the cytoplasm, whereas in Betz cells, by contrast, the loss of normal nuclear TDP-43 expression remains mostly unaccompanied by the development of cytoplasmic aggregations...
January 2017: Acta Neuropathologica
https://www.readbyqxmd.com/read/27503823/-an-autopsy-case-of-globular-glial-tauopathy-presenting-with-amyotrophic-lateral-sclerosis-with-dementia
#11
Ryogen Sasaki, Maya Mimuro, Yasumasa Kokubo, Hiroshi Imai, Mari Yoshida, Hidekazu Tomimoto
We report an autopsy case of globular glial tauopathy (GGT) presenting clinically with amyotrophic lateral sclerosis (ALS) with dementia. A 79-year-old female developed weakness in the right upper limb, which progressed gradually. She developed apathy and speech disorder at 80 years of age. On neurological examination, she showed signs of upper and lower motor neuron disorder and dementia, but no extrapyramidal signs. The clinical diagnosis was ALS with dementia. The autopsy revealed left predominant marked atrophy of the frontal lobe due to severe neuronal loss and Gliosis...
August 2016: Brain and Nerve, Shinkei Kenkyū No Shinpo
https://www.readbyqxmd.com/read/27488962/normal-stroma-suppresses-cancer-cell-proliferation-via-mechanosensitive-regulation-of-jmjd1a-mediated-transcription
#12
Riina Kaukonen, Anja Mai, Maria Georgiadou, Markku Saari, Nicola De Franceschi, Timo Betz, Harri Sihto, Sami Ventelä, Laura Elo, Eija Jokitalo, Jukka Westermarck, Pirkko-Liisa Kellokumpu-Lehtinen, Heikki Joensuu, Reidar Grenman, Johanna Ivaska
Tissue homeostasis is dependent on the controlled localization of specific cell types and the correct composition of the extracellular stroma. While the role of the cancer stroma in tumour progression has been well characterized, the specific contribution of the matrix itself is unknown. Furthermore, the mechanisms enabling normal-not cancer-stroma to provide tumour-suppressive signals and act as an antitumorigenic barrier are poorly understood. Here we show that extracellular matrix (ECM) generated by normal fibroblasts (NFs) is softer than the CAF matrix, and its physical and structural features regulate cancer cell proliferation...
2016: Nature Communications
https://www.readbyqxmd.com/read/27406873/prt062607-achieves-complete-inhibition-of-the-spleen-tyrosine-kinase-at-tolerated-exposures-following-oral-dosing-in-healthy-volunteers
#13
Greg Coffey, Aradhana Rani, Andreas Betz, Yvonne Pak, Helena Haberstock-Debic, Anjali Pandey, Stanley Hollenbach, Daniel D Gretler, Tim Mant, Stipo Jurcevic, Uma Sinha
The spleen tyrosine kinase (SYK) regulates immune cell activation in response to engagement of a variety of receptors, making it an intriguing target for the treatment of inflammatory and autoimmune disorders as well as certain B-cell malignancies. We have previously reported on the discovery and preclinical characterization of PRT062607, a potent and highly selective inhibitor of SYK that exhibits robust anti-inflammatory activity in a variety of animal models. Here we present data from our first human studies aimed at characterizing the pharmacokinetics (PK), pharmacodynamics (PD), and safety of PRT062607 in healthy volunteers following single and multiple oral administrations...
February 2017: Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/27385398/impact-of-comorbidity-and-anemia-in-patients-with-oropharyngeal-cancer-primarily-treated-with-surgery-in-the-human-papillomavirus-era
#14
Philipp Baumeister, Josepha Rauch, Christian Jacobi, Ulrich Kisser, Christian Betz, Sven Becker, Maximilian Reiter
BACKGROUND: Comorbidity and anemia strongly influence treatment and outcome of oropharyngeal squamous cell carcinomas (SCCs), but respective data are frequently lacking in clinical studies. We evaluated 149 cases of oropharyngeal SCC primarily treated with surgery regarding the impact of these factors on disease-free survival (DFS) and overall survival (OS). METHODS: Data were recorded during treatment and follow-up visits. Human papillomavirus (HPV) association of oropharyngeal SCC was detected by p16 immunohistochemistry...
January 2017: Head & Neck
https://www.readbyqxmd.com/read/27381223/cell-behaviors-and-dynamics-during-angiogenesis
#15
REVIEW
Charles Betz, Anna Lenard, Heinz-Georg Belting, Markus Affolter
Vascular networks are formed and maintained through a multitude of angiogenic processes, such as sprouting, anastomosis and pruning. Only recently has it become possible to study the behavior of the endothelial cells that contribute to these networks at a single-cell level in vivo This Review summarizes what is known about endothelial cell behavior during developmental angiogenesis, focusing on the morphogenetic changes that these cells undergo.
July 1, 2016: Development
https://www.readbyqxmd.com/read/27378156/cell-sized-liposome-doublets-reveal-active-tension-build-up-driven-by-acto-myosin-dynamics
#16
V Caorsi, J Lemière, C Campillo, M Bussonnier, J Manzi, T Betz, J Plastino, K Carvalho, C Sykes
Cells modulate their shape to fulfill specific functions, mediated by the cell cortex, a thin actin shell bound to the plasma membrane. Myosin motor activity, together with actin dynamics, contributes to cortical tension. Here, we examine the individual contributions of actin polymerization and myosin activity to tension increase with a non-invasive method. Cell-sized liposome doublets are covered with either a stabilized actin cortex of preformed actin filaments, or a dynamic branched actin network polymerizing at the membrane...
July 20, 2016: Soft Matter
https://www.readbyqxmd.com/read/27373764/an-expedited-approach-for-sustained-delivery-of-bone-morphogenetic-protein-7-to-bone-defects-using-gene-activated-fragments-of-subcutaneous-fat
#17
Volker M Betz, Oliver B Betz, Tom Rosin, Alexander Keller, Christian Thirion, Michael Salomon, Suzanne Manthey, Peter Augat, Volkmar Jansson, Peter E Müller, Stefan Rammelt, Hans Zwipp
BACKGROUND: Delivery of bone morphogenetic protein-7 (BMP-7) to bone defects can be improved by applying gene transfer methods. However, traditional ex vivo gene therapy approaches are cumbersome and costly, requiring the extraction and culturing of cells. Therefore, we evaluated a novel, expedited ex vivo BMP-7 gene transfer technology based on the use of fragments of subcutaneous fat tissue. METHODS: We created 5-mm mid-femoral bone defects in the right femora of 23 male, syngeneic Fischer 344 rats...
August 2016: Journal of Gene Medicine
https://www.readbyqxmd.com/read/27352359/sensory-and-motor-cortex-function-contributes-to-symptom-severity-in-spinocerebellar-ataxia-type-6
#18
Nyeonju Kang, Evangelos A Christou, Roxana G Burciu, Jae Woo Chung, Jesse C DeSimone, Edward Ofori, Tetsuo Ashizawa, Sankarasubramon H Subramony, David E Vaillancourt
Spinocerebellar ataxia type 6 (SCA6) is a genetic disease that causes degeneration of Purkinje cells, and recent evidence points to degeneration of Betz cells in the motor cortex. The relation between functional activity of motor cortex and symptom severity during a hand-grip motor control in vivo has not yet been investigated. This study explored both functional changes in the sensorimotor cortex and cerebellar regions and structural alterations in the cerebellum for SCA6 patients as compared to age-matched healthy controls using a multimodal imaging approach (task-based fMRI, task-based functional connectivity, and free-water diffusion MRI)...
June 28, 2016: Brain Structure & Function
https://www.readbyqxmd.com/read/27271249/tensile-forces-originating-from-cancer-spheroids-facilitate-tumor-invasion
#19
Katarzyna S Kopanska, Yara Alcheikh, Ralitza Staneva, Danijela Vignjevic, Timo Betz
The mechanical properties of tumors and the tumor environment provide important information for the progression and characterization of cancer. Tumors are surrounded by an extracellular matrix (ECM) dominated by collagen I. The geometrical and mechanical properties of the ECM play an important role for the initial step in the formation of metastasis, presented by the migration of malignant cells towards new settlements as well as the vascular and lymphatic system. The extent of this cell invasion into the ECM is a key medical marker for cancer prognosis...
2016: PloS One
https://www.readbyqxmd.com/read/27234382/activating-kras-mutations-are-characteristic-of-oncocytic-sinonasal-papilloma-and-associated-sinonasal-squamous-cell-carcinoma
#20
Aaron M Udager, Jonathan B McHugh, Bryan L Betz, Kathleen T Montone, Virginia A Livolsi, Raja R Seethala, Evgeny Yakirevich, O Hans Iwenofu, Bayardo Perez-Ordonez, Kathleen E DuRoss, Helmut C Weigelin, Megan S Lim, Kojo Sj Elenitoba-Johnson, Noah A Brown
Oncocytic sinonasal papillomas (OSPs) are benign tumours of the sinonasal tract, a subset of which are associated with synchronous or metachronous sinonasal squamous cell carcinoma (SNSCC). Activating EGFR mutations were recently identified in nearly 90% of inverted sinonasal papillomas (ISPs) - a related tumour with distinct morphology. EGFR mutations were, however, not found in OSP, suggesting that different molecular alterations drive the oncogenesis of these tumours. In this study, tissue from 51 cases of OSP and five cases of OSP-associated SNSCC was obtained retrospectively from six institutions...
August 2016: Journal of Pathology
keyword
keyword
67587
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"