keyword
https://read.qxmd.com/read/38531230/generation-and-characterization-of-an-ips-cell-line-pumci006-a-from-skin-fibroblasts-of-a-patient-with-an-m239t-mutation-in-psen2-gene
#21
JOURNAL ARTICLE
Wanwan Zhu, Jinjuan Zhou, Li Shang, You Zhou, Qingyu Wang, Yuesong Yu, Liling Dong, Chenhui Mao, Shanshan Chu, Wei Jin, Jie Li, Jing Gao
Presenilin-2 (PSEN2) mutation is one of the pathogenic factors of autosomal dominant early-onset Alzheimer's disease (EOAD). We generated a human induced pluripotent stem cell (iPSC) line from fibroblasts of an EOAD patient carrying PSEN2 mutation (c.716 T > C) utilizing Sendai reprogramming kit. The resulting iPSC line carried patient-specific point mutation, exhibited typical iPSC morphology, retained a normal karyotype, expressed pluripotency markers, and could form embryoid bodies. Established iPSC line serve as valuable resource for EOAD disease pathogenesis modelling and drug screening...
March 14, 2024: Stem Cell Research
https://read.qxmd.com/read/38531229/an-achievement-has-been-made-in-establishing-an-induced-pluripotent-stem-cell-line-sdphi005-a-from-a-healthy-chinese-male-donor
#22
JOURNAL ARTICLE
Jing Shao, Yan Jin, Chunhong Shao, Xiaoqiao Zhang, Guang Yang
Successfully transforming isolated peripheral blood mononuclear cells (PBMCs) from a healthy male into induced pluripotent stem cells (iPSCs) was achieved using non-integrating episomal vectors containing OCT4, SOX2, c-MYC, KLF4, and BCL-XL. The iPSCs exhibited a normal karyotype, expressed pluripotency markers, and demonstrated the ability to differentiate into three germ layers in vitro. This iPSC line may be employed for subsequent research purposes.
March 16, 2024: Stem Cell Research
https://read.qxmd.com/read/38530829/microbundlecompute-automated-segmentation-tracking-and-analysis-of-subdomain-deformation-in-cardiac-microbundles
#23
JOURNAL ARTICLE
Hiba Kobeissi, Javiera Jilberto, M Çağatay Karakan, Xining Gao, Samuel J DePalma, Shoshana L Das, Lani Quach, Jonathan Urquia, Brendon M Baker, Christopher S Chen, David Nordsletten, Emma Lejeune
Advancing human induced pluripotent stem cell derived cardiomyocyte (hiPSC-CM) technology will lead to significant progress ranging from disease modeling, to drug discovery, to regenerative tissue engineering. Yet, alongside these potential opportunities comes a critical challenge: attaining mature hiPSC-CM tissues. At present, there are multiple techniques to promote maturity of hiPSC-CMs including physical platforms and cell culture protocols. However, when it comes to making quantitative comparisons of functional behavior, there are limited options for reliably and reproducibly computing functional metrics that are suitable for direct cross-system comparison...
2024: PloS One
https://read.qxmd.com/read/38530240/induction-of-immortal-like-and-functional-car-t-cells-by-defined-factors
#24
JOURNAL ARTICLE
Lixia Wang, Gang Jin, Qiuping Zhou, Yanyan Liu, Xiaocui Zhao, Zhuoyang Li, Na Yin, Min Peng
Long-term antitumor efficacy of chimeric antigen receptor (CAR) T cells depends on their functional persistence in vivo. T cells with stem-like properties show better persistence, but factors conferring bona fide stemness to T cells remain to be determined. Here, we demonstrate the induction of CAR T cells into an immortal-like and functional state, termed TIF. The induction of CARTIF cells depends on the repression of two factors, BCOR and ZC3H12A, and requires antigen or CAR tonic signaling. Reprogrammed CARTIF cells possess almost infinite stemness, similar to induced pluripotent stem cells while retaining the functionality of mature T cells, resulting in superior antitumor effects...
May 6, 2024: Journal of Experimental Medicine
https://read.qxmd.com/read/38530081/making-human-hematopoietic-stem-cells-without-transgenes
#25
Luis G Palma, Anna Bigas
Creating hematopoietic stem cells (HSCs) capable of multilineage engraft while possessing the ability to self-renew stands as a pivotal achievement within the field of regenerative medicine. However, achieving the generation of these cells without transgene expression or teratoma formation has not been fully accomplished. In a recent publication featured in Cell Stem Cell , Piau et al. document the production of functional HSCs derived from human-induced pluripotent stem cells (hiPSCs). They achieved this through a one-step differentiation protocol that notably does not require any transgene expression...
March 26, 2024: Cellular Reprogramming
https://read.qxmd.com/read/38529852/parenteral-nutrition-emulsion-inhibits-cyp3a4-in-an-ipsc-derived-liver-organoids-testing-platform
#26
JOURNAL ARTICLE
Sean P Harrison, Saphira F Baumgarten, Maria E Chollet, Benedicte Stavik, Anindita Bhattacharya, Runar Almaas, Gareth J Sullivan
OBJECTIVES: Parenteral nutrition (PN) is used for patients of varying ages with intestinal failure to supplement calories. Premature newborns with low birth weight are at a high risk for developing PN associated liver disease (PNALD) including steatosis, cholestasis, and gallbladder sludge/stones. To optimize nutrition regimens, models are required to predict PNALD. METHODS: We have exploited induced pluripotent stem cell derived liver organoids to provide a testing platform for PNALD...
March 26, 2024: Journal of Pediatric Gastroenterology and Nutrition
https://read.qxmd.com/read/38529661/uracil-and-pyridine-containing-hdac-inhibitors-displayed-cytotoxicity-in-colorectal-and-glioblastoma-cancer-stem-cells
#27
JOURNAL ARTICLE
Francesco Fiorentino, Emanuele Fabbrizi, Alessia Raucci, Beatrice Noce, Rossella Fioravanti, Sergio Valente, Chantal Paolini, Ruggero De Maria, Christian Steinkühler, Paola Gallinari, Dante Rotili, Antonello Mai
Cancer stem cells (CSCs) are a niche of highly tumorigenic cells featuring self-renewal, activation of pluripotency genes, multidrug resistance, and ability to cause cancer relapse. Seven HDACi (1-7), showing either hydroxamate or 2'-aminoanilide function, were tested in colorectal cancer (CRC) and glioblastoma multiforme (GBM) CSCs to determine their effects on cell proliferation, H3 acetylation levels and in-cell HDAC activity. Two uracil-based hydroxamates, 5 and 6, which differ in substitution at C5 and C6 positions of the pyrimidine ring, exhibited the greatest cytotoxicity in GBM (5) and CRC (6) CSCs, followed by the pyridine-hydroxamate 2, with 2- to 6-fold higher potency than the positive control SAHA...
March 26, 2024: ChemMedChem
https://read.qxmd.com/read/38529501/comparative-study-of-enriched-dopaminergic-neurons-from-siblings-with-gaucher-disease-discordant-for-parkinsonism
#28
Ellen Hertz, Gani Perez, Ying Hao, Krystyna Rytel, Charis Ma, Martha Kirby, Stacie Anderson, Stephen Wincovitch, Kate Andersh, Tim Ahfeldt, Joel Blanchard, Yue Andy Qi, Grisel Lopez, Nahid Tayebi, Ellen Sidransky, Yu Chen
Inducible pluripotent stem cells (iPSCs) derived from patient samples have significantly enhanced our ability to model neurological diseases. Comparative studies of dopaminergic (DA) neurons differentiated from iPSCs derived from siblings with Gaucher disease discordant for parkinsonism provides a valuable avenue to explore genetic modifiers contributing to GBA1 -associated parkinsonism in disease-relevant cells. However, such studies are often complicated by the inherent heterogeneity in differentiation efficiency among iPSC lines derived from different individuals...
February 28, 2024: bioRxiv
https://read.qxmd.com/read/38528578/matrix-free-human-pluripotent-stem-cell-manufacturing-by-seed-train-approach-and-intermediate-cryopreservation
#29
JOURNAL ARTICLE
Kevin Ullmann, Felix Manstein, Wiebke Triebert, Nils Kriedemann, Annika Franke, Jana Teske, Mira Mertens, Victoria Lupanow, Gudrun Göhring, Alexandra Haase, Ulrich Martin, Robert Zweigerdt
BACKGROUND: Human pluripotent stem cells (hPSCs) have an enormous therapeutic potential, but large quantities of cells will need to be supplied by reliable, economically viable production processes. The suspension culture (three-dimensional; 3D) of hPSCs in stirred tank bioreactors (STBRs) has enormous potential for fuelling these cell demands. In this study, the efficient long-term matrix-free suspension culture of hPSC aggregates is shown. METHODS AND RESULTS: STBR-controlled, chemical aggregate dissociation and optimized passage duration of 3 or 4 days promotes exponential hPSC proliferation, process efficiency and upscaling by a seed train approach...
March 25, 2024: Stem Cell Research & Therapy
https://read.qxmd.com/read/38528561/transcriptome-and-open-chromatin-analysis-reveals-the-process-of-myocardial-cell-development-and-key-pathogenic-target-proteins-in-long-qt-syndrome-type-7
#30
JOURNAL ARTICLE
Peipei Chen, Junyu Long, Tianrui Hua, Zhifa Zheng, Ying Xiao, Lianfeng Chen, Kang Yu, Wei Wu, Shuyang Zhang
OBJECTIVE: Long QT syndrome type 7 (Andersen-Tawil syndrome, ATS), which is caused by KCNJ2 gene mutation, often leads to ventricular arrhythmia, periodic paralysis and skeletal malformations. The development, differentiation and electrophysiological maturation of cardiomyocytes (CMs) changes promote the pathophysiology of Long QT syndrome type 7(LQT7). We aimed to specifically reproduce the ATS disease phenotype and study the pathogenic mechanism. METHODS AND RESULTS: We established a cardiac cell model derived from human induced pluripotent stem cells (hiPSCs) to the phenotypes and electrophysiological function, and the establishment of a human myocardial cell model that specifically reproduces the symptoms of ATS provides a reliable platform for exploring the mechanism of this disease or potential drugs...
March 25, 2024: Journal of Translational Medicine
https://read.qxmd.com/read/38527856/app-antisense-oligonucleotides-reduce-a%C3%AE-aggregation-and-rescue-endolysosomal-dysfunction-in-alzheimer-s-disease
#31
JOURNAL ARTICLE
Christy Hung, Emre Fertan, Frederick J Livesey, David Klenerman, Rickie Patani
APP gene dosage is strongly associated with Alzheimer's disease (AD) pathogenesis. Genomic duplication of the APP locus leads to autosomal dominant early-onset AD. Individuals with Down syndrome (trisomy of chromosome 21) harbor 3 copies of the APP gene and invariably develop progressive AD with highly characteristic neuropathological features. Restoring expression of APP to the equivalent of that of two gene copies, or lower, is a rational therapeutic strategy, as it would restore physiological levels of neuronal APP protein without the potentially deleterious consequences of inadvertently inducing loss of APP function...
March 25, 2024: Brain
https://read.qxmd.com/read/38527279/synergistic-effect-of-electrical-and-biochemical-stimulation-on-human-ipsc-derived-neural-differentiation-in-a-microfluidic-electrode-array-chip
#32
JOURNAL ARTICLE
Na Yeon Kim, Yoon Young Choi, Tae Hyeon Kim, Jang Ho Ha, Tae-Hyung Kim, Taewook Kang, Bong Geun Chung
Neural differentiation is crucial for advancing our understanding of the nervous system and developing treatments for neurological disorders. The advanced methods and the ability to manipulate the alignment, proliferation, and differentiation of stem cells are essential for studying neuronal development and synaptic interactions. However, the utilization of human induced pluripotent stem cells (iPSCs) for disease modeling of neurodegenerative conditions may be constrained by the prolonged duration and uncontrolled cell differentiation required for functional neural cell differentiation...
March 25, 2024: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38526281/cell-reprogramming-therapy-for-parkinson-s-disease
#33
JOURNAL ARTICLE
Wenjing Dong, Shuyi Liu, Shangang Li, Zhengbo Wang
Parkinson's disease is typically characterized by the progressive loss of dopaminergic neurons in the substantia nigra pars compacta. Many studies have been performed based on the supplementation of lost dopaminergic neurons to treat Parkinson's disease. The initial strategy for cell replacement therapy used human fetal ventral midbrain and human embryonic stem cells to treat Parkinson's disease, which could substantially alleviate the symptoms of Parkinson's disease in clinical practice. However, ethical issues and tumor formation were limitations of its clinical application...
November 1, 2024: Neural Regeneration Research
https://read.qxmd.com/read/38526223/crispri-a-way-to-integrate-ipsc-derived-neuronal-models
#34
JOURNAL ARTICLE
Sarah N J Franks, Rachel Heon-Roberts, Brent J Ryan
The genetic landscape of neurodegenerative diseases encompasses genes affecting multiple cellular pathways which exert effects in an array of neuronal and glial cell-types. Deconvolution of the roles of genes implicated in disease and the effects of disease-associated variants remains a vital step in the understanding of neurodegeneration and the development of therapeutics. Disease modelling using patient induced pluripotent stem cells (iPSCs) has enabled the generation of key cell-types associated with disease whilst maintaining the genomic variants that predispose to neurodegeneration...
March 25, 2024: Biochemical Society Transactions
https://read.qxmd.com/read/38526136/isolation-of-diverse-simian-arteriviruses-causing-hemorrhagic-disease
#35
JOURNAL ARTICLE
Teressa M Shaw, Samuel T Dettle, Andres Mejia, Jennifer M Hayes, Heather A Simmons, Puja Basu, Jens H Kuhn, Mitchell D Ramuta, Cody J Warren, Peter B Jahrling, David H O'Connor, Liupei Huang, Misbah Zaeem, Jiwon Seo, Igor I Slukvin, Matthew E Brown, Adam L Bailey
Genetically diverse simian arteriviruses (simarteriviruses) naturally infect geographically and phylogenetically diverse monkeys, and cross-species transmission and emergence are of considerable concern. Characterization of most simarteriviruses beyond sequence analysis has not been possible because the viruses fail to propagate in the laboratory. We attempted to isolate 4 simarteriviruses, Kibale red colobus virus 1, Pebjah virus, simian hemorrhagic fever virus, and Southwest baboon virus 1, by inoculating an immortalized grivet cell line (known to replicate simian hemorrhagic fever virus), primary macaque cells, macrophages derived from macaque induced pluripotent stem cells, and mice engrafted with macaque CD34+-enriched hematopoietic stem cells...
April 2024: Emerging Infectious Diseases
https://read.qxmd.com/read/38526084/recording-and-analyzing-multimodal-large-scale-neuronal-ensemble-dynamics-on-cmos-integrated-high-density-microelectrode-array
#36
JOURNAL ARTICLE
Brett Addison Emery, Shahrukh Khanzada, Xin Hu, Diana Klütsch, Hayder Amin
Large-scale neuronal networks and their complex distributed microcircuits are essential to generate perception, cognition, and behavior that emerge from patterns of spatiotemporal neuronal activity. These dynamic patterns emerging from functional groups of interconnected neuronal ensembles facilitate precise computations for processing and coding multiscale neural information, thereby driving higher brain functions. To probe the computational principles of neural dynamics underlying this complexity and investigate the multiscale impact of biological processes in health and disease, large-scale simultaneous recordings have become instrumental...
March 8, 2024: Journal of Visualized Experiments: JoVE
https://read.qxmd.com/read/38526067/past-and-future-of-alveolar-organoids-for-lung-regenerative-medicine
#37
JOURNAL ARTICLE
Ryuta Mikawa, Shimpei Gotoh
The lung is regarded as having limited regenerative capacity, and there are few treatment options for refractory lung diseases, such as interstitial pneumonia. Lung transplantation is the final option available in some scenarios. Research in this area has been slow owing to the complex structure of the lung for efficient gas exchange between the alveolar spaces and capillaries as well as the difficulty in obtaining specimens from patients with progressive lung disease. However, basic research over the past decade in the field of mouse and human embryology using genetic lineage tracing techniques and stem cell biology using primary and pluripotent stem cell-derived alveolar organoids has begun to clarify the tissue response in various intractable lung diseases and the mechanisms underlying remodeling...
March 25, 2024: Stem Cells
https://read.qxmd.com/read/38525786/dna-methylation-markers-for-risk-of-metastasis-in-a-cohort-of-men-with-localized-prostate-cancer
#38
JOURNAL ARTICLE
Talar S Habeshian, Kimberly L Cannavale, Jeff M Slezak, Yu-Hsiang Shu, Gary W Chien, XuFeng Chen, Feng Shi, Kimberly D Siegmund, Stephen K Van Den Eeden, Jiaoti Huang, Chun R Chao
Accurately identifying life-threatening prostate cancer (PCa) at time of diagnosis remains an unsolved problem. We evaluated whether DNA methylation status of selected candidate genes can predict the risk of metastasis beyond clinical risk factors in men with untreated PCa. A nested case-control study was conducted among men diagnosed with localized PCa at Kaiser Permanente California between 01/01/1997-12/31/2006 who did not receive curative treatments. Cases were those who developed metastasis within 10 years from diagnosis...
December 2024: Epigenetics: Official Journal of the DNA Methylation Society
https://read.qxmd.com/read/38525239/diverting-the-food-freezing-technology-improves-the-cryopreservation-efficiency-of-induced-pluripotent-stem-cells-and-derived-neurospheres
#39
JOURNAL ARTICLE
Kenzo Bamba, Midori Ozawa, Hiroaki Daitoku, Arihiro Kohara
INTRODUCTION: Recent advances in induced pluripotent stem (iPS) technology and regenerative medicine require effective cryopreservation of iPSC-derived differentiated cells and three-dimensional cell aggregates (eg. Spheroids and organoids). Moreover, innovative freezing technologies for keeping food fresh over the long-term rapidly developed in the food industry. Therefore, we examined whether one of such freezing technologies, called "Dynamic Effect Powerful Antioxidation Keeping (DEPAK)," could be effective for the cryopreservation of biological materials...
December 2024: Regenerative Therapy
https://read.qxmd.com/read/38523789/cortical-brain-organoid-slices-cbos-for-the-study-of-human-neural-cells-in-minimal-networks
#40
JOURNAL ARTICLE
Laura Petersilie, Sonja Heiduschka, Joel S E Nelson, Louis A Neu, Stephanie Le, Ruchika Anand, Karl W Kafitz, Alessandro Prigione, Christine R Rose
Brain organoids derived from human pluripotent stem cells are a promising tool for studying human neurodevelopment and related disorders. Here, we generated long-term cultures of cortical brain organoid slices (cBOS) grown at the air-liquid interphase from regionalized cortical organoids. We show that cBOS host mature neurons and astrocytes organized in complex architecture. Whole-cell patch-clamp demonstrated subthreshold synaptic inputs and action potential firing of neurons. Spontaneous intracellular calcium signals turned into synchronous large-scale oscillations upon combined disinhibition of NMDA receptors and blocking of GABAA receptors...
April 19, 2024: IScience
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