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https://www.readbyqxmd.com/read/29031129/a-dual-type-responsive-electrochemical-immunosensor-for-quantitative-detection-of-pcsk9-based-on-n-c60-pdpt-n-gnrs-and-pt-poly-methylene-blue-nanocomposites
#1
Yan Li, Junlin He, Jun Chen, Yazhen Niu, Yilin Zhao, Yuchan Zhang, Chao Yu
In this study, a dual-type responsive electrochemical immunosensor was developed for the quantitative detection of proprotein convertase subtilisin/kexin type 9 (PCSK9), a potential biomarker of cardiovascular disease in serum. N-doped graphene nanoribbons (N-GNRs) with good conductivity were used as the sensing matrix modifying the glassy carbon electrode. Palladium platinum alloy (PdPt) nanoparticles with high catalytic performance toward the reduction of hydrogen peroxide (H2O2) were reduced onto amino-functionalized fullerene (n-C60-PdPt) and significantly amplified the electrochemical signal recorded by the amperometric i-t curve...
September 28, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29028305/fast-image-guided-stratification-using-anti-programmed-death-ligand-1-gold-nanoparticles-for-cancer-immunotherapy
#2
Rinat Meir, Katerina Shamalov, Tamar Sadan, Menachem Motiei, Gur Yaari, Cyrille J Cohen, Rachela Popovtzer
Cancer immunotherapy has made enormous progress in offering safer and more effective treatments for the disease. Specifically, programmed death-ligand 1 antibody (αPDL1), designed to perform immune checkpoint blockade (ICB), is now considered a pillar in cancer immunotherapy. However, due to the complexity and heterogeneity of tumors, as well as the diversity in patient response, ICB therapy has only a 30 percent success rate, at most; moreover, the efficacy of ICB can be evaluated only two months after start of treatment...
October 13, 2017: ACS Nano
https://www.readbyqxmd.com/read/29028294/carbon-dots-g-c3n4-nanoheterostructures-based-signal-generation-tags-for-photoelectrochemical-immunoassay-of-cancer-biomarkers-coupling-with-copper-nanoclusters
#3
Shuzhen Lv, Yi Li, Kangyao Zhang, Zhenzhen Lin, Dianping Tang
A class of 0-dimensional/2-dimensional (0D/2D) nanoheterostructures based on carbon quantum dots (CQDs) and graphitic carbon nitride (g-C3N4) was designed as the signal-generation tags for the sensitive photoelectrochemical (PEC) immunoassay of prostate-specific antigen (PSA) coupling with the copper nanoclusters (CuNCs). Combination of CQDs with g-C3N4 promoted the photoexcited electron/hole separation, and largely increased the photocurrents of the nanoheterostructures. Initially, a sandwich-type immunoreaction was carried out on monoclonal anti-PSA antibody-coated microplate by using PSA aptamer linked with CuNCs as the tracer...
October 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29027631/detrimental-roles-of-tnf-alpha-in-the-antiphospholipid-syndrome-and-de-novo-synthesis-of-antiphospholipid-antibodies-induced-by-biopharmaceuticals-against-tnf-alpha
#4
REVIEW
Mirjana Bećarević
Antiphospholipid syndrome (APS) is an autoimmune disease that is characterized by arterial and/or venous thrombosis and/or recurrent pregnancy losses. Obstetric APS (OAPS) is considered as a distinct entity from vascular APS (VAPS). In the absence of any additional disease, APS is designated as primary (PAPS), while the term secondary APS (SAPS) is used when other diseases are associated. Catastrophic APS (CAPS) is characterized by the rapid development of multiple thrombosis in various vital organs. The presence of antiphospholipid antibodies (aPL Abs) is considered as a laboratory criterion for APS diagnosis...
October 13, 2017: Journal of Thrombosis and Thrombolysis
https://www.readbyqxmd.com/read/29027395/prognostic-value-of-programmed-death-ligand-1-pd-l1-expression-in-ovarian-clear-cell-carcinoma
#5
Jun Zhu, Hao Wen, Rui Bi, Yong Wu, Xiaohua Wu
OBJECTIVE: Programmed death-ligand 1 (PD-L1) was expressed in various tumors and antibodies targeting its receptor programmed cell death-1 (PD-1) are emerging cancer therapeutics. This study was designed to evaluate the expression of PD-L1 and its correlation with clinicopathologic features and clinical outcomes in ovarian clear cell carcinoma (OCCC). METHODS: The PD-L1 expression was measured by tissue-microarray-based immunohistochemistry from 122 eligible patients diagnosed with OCCC...
November 2017: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/29027170/chromatin-immunoprecipitation-of-skeletal-muscle-tissue
#6
Amarjit Saini, Carl Johan Sundberg
Chromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between histone proteins and genomic DNA regions and transcriptional regulation using antibodies to enrich genomic regions associated with these epitopes. Either to monitor the presence of histones with post-translational modifications at specific genomic locations or to measure transcription factor interactions with a candidate target gene, protein-DNA complexes are most commonly crosslinked using formaldehyde, which stabilizes these transient interactions...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29027161/considerations-on-experimental-design-and-data-analysis-of-chromatin-immunoprecipitation-experiments
#7
Antonio Jordán-Pla, Neus Visa
Arguably one of the most valuable techniques to study chromatin organization, ChIP is the method of choice to map the contacts established between proteins and genomic DNA. Ever since its inception, more than 30 years ago, ChIP has been constantly evolving, improving, and expanding its capabilities and reach. Despite its widespread use by many laboratories across a wide variety of disciplines, ChIP assays can be sometimes challenging to design, and are often sensitive to variations in practical implementation...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29025325/versatility-of-targeted-antibiotic-loaded-gold-nanoconstructs-for-the-treatment-of-biofilm-associated-bacterial-infections
#8
Daniel G Meeker, Tengjiao Wang, Walter N Harrington, Vladimir P Zharov, Sarah A Johnson, Samir V Jenkins, Stephanie E Oyibo, Christopher M Walker, Weston B Mills, Mark E Shirtliff, Karen E Beenken, Jingyi Chen, Mark S Smeltzer
BACKGROUND: We previously demonstrated that a photoactivatable therapeutic approach employing antibiotic-loaded, antibody-conjugated, polydopamine (PDA)-coated gold nanocages (AuNCs) could be used for the synergistic killing of bacterial cells within a biofilm. The approach was validated with a focus on Staphylococcus aureus using an antibody specific for staphylococcal protein A (Spa) and an antibiotic (daptomycin) active against Gram-positive cocci including methicillin-resistant S...
October 12, 2017: International Journal of Hyperthermia
https://www.readbyqxmd.com/read/29023946/a-novel-recombinant-anti-egfr-peptide-vaccine-capable-of-active-immunization-and-reduction-of-tumor-volume-in-mice-model
#9
Majid Asadi-Ghalehni, Mohamad Javad Rasaee, Masoumeh RajabiBazl, Masood Khosravani, Majid Motaghinejad, Masoud Javanmardi, Saeed Khalili, Helmout Modjtahedi, Esmaeil Sadroddiny
Over-expression of the Epidermal Growth Factor Receptor (EGFR) has been reported in a number of human malignancies. High levels of expression of this receptor have been associated with poor survival in many such patients. Active immunizations that elicit antibodies of the desired type could be an appealing alternative for conventional passive immunization. In this regard, we decide to construct a novel recombinant peptide vaccine capable of prophylactic and therapeutic effects. A novel fusion recombinant peptide base vaccine consisting of L2 domain of murine ECD-EGFR and EGFR mimotope (EM-L2) was constructed and its prophylactic and therapeutic effects on the Lewis lung carcinoma mice (C57/BL6) model were evaluated...
October 10, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/29023549/supraphysiologic-control-over-hiv-1-replication-mediated-by-cd8-t-cells-expressing-a-re-engineered-cd4-based-chimeric-antigen-receptor
#10
Rachel S Leibman, Max W Richardson, Christoph T Ellebrecht, Colby R Maldini, Joshua A Glover, Anthony J Secreto, Irina Kulikovskaya, Simon F Lacey, Sarah R Akkina, Yanjie Yi, Farida Shaheen, Jianbin Wang, Keith A Dufendach, Michael C Holmes, Ronald G Collman, Aimee S Payne, James L Riley
HIV is adept at avoiding naturally generated T cell responses; therefore, there is a need to develop HIV-specific T cells with greater potency for use in HIV cure strategies. Starting with a CD4-based chimeric antigen receptor (CAR) that was previously used without toxicity in clinical trials, we optimized the vector backbone, promoter, HIV targeting moiety, and transmembrane and signaling domains to determine which components augmented the ability of T cells to control HIV replication. This re-engineered CAR was at least 50-fold more potent in vitro at controlling HIV replication than the original CD4 CAR, or a TCR-based approach, and substantially better than broadly neutralizing antibody-based CARs...
October 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29023483/a-simpler-and-more-cost-effective-peptide-biosynthetic-method-using-the-truncated-gst-as-carrier-for-epitope-mapping
#11
Wan-Xiang Xu, Jian Wang, Hai-Ping Tang, Ling-Han Chen, Wen-Bo Lian, Jian-Min Zhan, Satish K Gupta, Chao-Neng Ji, Shao-Hua Gu, Yi Xie
There is a need to develop better methods for epitope mapping and/or identification of antibody-recognizing motifs. Here, we describe improved biosynthetic peptide (BSP) method using a newly developed plasmid pXXGST-3 as vector, which has a viral E7 gene in the cloning sites of pXXGST-1. It is crucial to employ pXXGST-3 instead of pXXGST-1, since it makes use of the BSP method simpler and easier to perform, and more cost-effective for epitope mapping. These merits are embodied in two aspects: i) convenient recovery of double enzyme-digested product due to the existence of 315 bp inserted between BamH I and Sal I sites, and thus greatly reducing the production of self-ligation clones, and ii) no longer requiring control protein when screening recombinant (r-) clones expressing 8/18mer peptides by running polyacrylamide gel electrophoresis...
2017: PloS One
https://www.readbyqxmd.com/read/29023479/evaluating-the-efficiency-of-chef-and-cmv-promoter-with-ires-and-furin-2a-linker-sequences-for-monoclonal-antibody-expression-in-cho-cells
#12
Saeedeh Ebadat, Samira Ahmadi, Maryam Ahmadi, Fatemeh Nematpour, Farzaneh Barkhordari, Reza Mahdian, Fatemeh Davami, Fereidoun Mahboudi
In recent years, monoclonal antibodies (mAbs) have been developed as powerful therapeutic and diagnostic agents and Chinese hamster ovary (CHO) cells have emerged as the dominant host for the recombinant expression of these proteins. A critical step in recombinant expression is the utilization of strong promoters, such as the Chinese Hamster Elongation Factor-1α (CHEF-1) promoter. To compare the strengths of CHEF with cytomegalovirus (CMV) promoter for mAb expression in CHO cells, four bicistronic vectors bearing either internal ribosome entry site (IRES) or Furin/2A (F2A) sequences were designed...
2017: PloS One
https://www.readbyqxmd.com/read/29022615/principles-of-mucin-structure-implications-for-the-rational-design-of-cancer-vaccines-derived-from-muc1-glycopeptides
#13
Nuria Martínez-Sáez, Jesús M Peregrina, Francisco Corzana
Cancer is currently one of the world's most serious public health problems. Significant efforts are being made to develop new strategies that can eradicate tumours selectively without detrimental effects to healthy cells. One promising approach is focused on the design of vaccines that contain partially glycosylated mucins in their formulation. Although some of these vaccines are in clinical trials, a lack of knowledge about the molecular basis that governs the antigen presentation, and the interactions between antigens and the elicited antibodies has limited their success thus far...
October 12, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/29021918/protection-of-calves-by-a-prefusion-stabilized-bovine-rsv-f-vaccine
#14
Baoshan Zhang, Lei Chen, Chiara Silacci, Michelle Thom, Jeffrey C Boyington, Aliaksandr Druz, M Gordon Joyce, Efrain Guzman, Wing-Pui Kong, Yen-Ting Lai, Guillaume B E Stewart-Jones, Yaroslav Tsybovsky, Yongping Yang, Tongqing Zhou, Ulrich Baxa, John R Mascola, Davide Corti, Antonio Lanzavecchia, Geraldine Taylor, Peter D Kwong
Bovine respiratory syncytial virus, a major cause of respiratory disease in calves, is closely related to human RSV, a leading cause of respiratory disease in infants. Recently, promising human RSV-vaccine candidates have been engineered that stabilize the metastable fusion (F) glycoprotein in its prefusion state; however, the absence of a relevant animal model for human RSV has complicated assessment of these vaccine candidates. Here, we use a combination of structure-based design, antigenic characterization, and X-ray crystallography to translate human RSV F stabilization into the bovine context...
March 8, 2017: NPJ Vaccines
https://www.readbyqxmd.com/read/29021793/intracellular-crosslinking-of-filoviral-nucleoproteins-with-xintrabodies-restricts-viral-packaging
#15
Tamarand Lee Darling, Laura Jo Sherwood, Andrew Hayhurst
Viruses assemble large macromolecular repeat structures that become part of the infectious particles or virions. Ribonucleocapsids (RNCs) of negative strand RNA viruses are a prime example where repetition of nucleoprotein (NP) along the genome creates a core polymeric helical scaffold that accommodates other nucleocapsid proteins including viral polymerase. The RNCs are transported through the cytosol for packaging into virions through association with viral matrix proteins at cell membranes. We hypothesized that RNC would be ideal targets for crosslinkers engineered to promote aberrant protein-protein interactions, thereby blocking their orderly transport and packaging...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29021394/hiv-1-gp120-protein-and-mvagp140-boost-immunogens-increase-immunogenicity-of-a-dna-mva-hiv-1-vaccine
#16
Xiaoying Shen, Rahul Basu, Sheetal Sawant, David Beaumont, Sue Fen Kwa, Celia LaBranche, Kelly E Seaton, Nicole L Yates, David C Montefiori, Guido Ferrari, Linda S Wyatt, Bernard Moss, S Munir Alam, Barton F Haynes, Georgia D Tomaras, Harriet L Robinson
An important goal of human immunodeficiency virus (HIV) vaccine design is identification of strategies that elicit effective antiviral humoral immunity. One novel approach comprises priming with DNA and boosting with modified vaccinia Ankara (MVA) expressing HIV-1 Env on virus like particles. Here we evaluated whether the addition of a gp120 protein in alum or MVA expressed secreted gp140 (MVAgp140) could improve immunogenicity of a DNA prime - MVA boost vaccine. Five rhesus macaques per group received two DNA primes at weeks 0 and 8 followed by three MVA boosts (with or without additional protein or MVAgp140) at weeks 18, 26 and 40...
October 11, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29020160/immune-cross-opsonization-within-emm-clusters-following-group-a-streptococcus-skin-infection-broadening-the-scope-of-type-specific-immunity
#17
Hannah R Frost, Delphine Laho, Martina L Sanderson-Smith, Paul Licciardi, Susan Donath, Nigel Curtis, Joseph Kado, James B Dale, Andrew C Steer, Pierre R Smeesters
Background: Group A Streptococcus (GAS) skin infections are particularly prevalent in developing nations. The GAS M protein, by which strains are differentiated into >220 different emm types, is immunogenic and elicits protective antibodies. A major obstacle for vaccine development has been the traditional understanding that immunity following infection is restricted to a single emm type. However, recent evidence has led to the hypothesis of immune cross-reactivity between emm types...
July 19, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29018438/antibody-immunity-induced-by-h7n9-avian-influenza-vaccines-evaluation-criteria-affecting-factors-and-implications-for-rational-vaccine-design
#18
Zenglei Hu, Xinan Jiao, Xiufan Liu
Severe H7N9 avian influenza virus (AIV) infections in humans have public health authorities around the world on high alert for the potential development of a human influenza pandemic. Currently, the newly-emerged highly pathogenic avian influenza A (H7N9) virus poses a dual challenge for public health and poultry industry. Numerous H7N9 vaccine candidates have been generated using various platforms. Immunization trials in animals and humans showed that H7N9 vaccines are apparently poorly immunogenic because they induced low hemagglutination inhibition and virus neutralizing antibody titers...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29018431/depression-of-vaccinal-immunity-to-marek-s-disease-by-infection-with-chicken-infectious-anemia-virus
#19
Yankun Zhang, Ning Cui, Ni Han, Jiayan Wu, Zhizhong Cui, Shuai Su
Marek's disease (MD) has been occurring with increasing frequency in chickens in recent years. To our knowledge, however, there has been no report of the very virulent plus (vv+) MD virus (MDV) field isolate in China. Studies have shown that dual infection with immunosuppressive viruses such as chicken infectious anemia virus (CIAV) occurs frequently in chickens developing MD. In this study, we performed a designed set of in vivo experiments, which comprised five different groups of chickens, including the group of CVI988/Rispens-vaccinated chickens, the groups of CVI988/Rispens-vaccinated chickens infected with MDV or CIAV or both viruses (MDV and CIAV), and the group of MDV-challenged chickens...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29018324/design-and-characterization-of-a-human-monoclonal-antibody-that-modulates-mutant-connexin-26-hemichannels-implicated-in-deafness-and-skin-disorders
#20
Liang Xu, Andrea Carrer, Francesco Zonta, Zhihu Qu, Peixiang Ma, Sheng Li, Federico Ceriani, Damiano Buratto, Giulia Crispino, Veronica Zorzi, Gaia Ziraldo, Francesca Bruno, Chiara Nardin, Chiara Peres, Flavia Mazzarda, Anna M Salvatore, Marcello Raspa, Ferdinando Scavizzi, Youjun Chu, Sichun Xie, Xuemei Yang, Jun Liao, Xiao Liu, Wei Wang, Shanshan Wang, Guang Yang, Richard A Lerner, Fabio Mammano
Background: Mutations leading to changes in properties, regulation, or expression of connexin-made channels have been implicated in 28 distinct human hereditary diseases. Eight of these result from variants of connexin 26 (Cx26), a protein critically involved in cell-cell signaling in the inner ear and skin. Lack of non-toxic drugs with defined mechanisms of action poses a serious obstacle to therapeutic interventions for diseases caused by mutant connexins. In particular, molecules that specifically modulate connexin hemichannel function without affecting gap junction channels are considered of primary importance for the study of connexin hemichannel role in physiological as well as pathological conditions...
2017: Frontiers in Molecular Neuroscience
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