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https://www.readbyqxmd.com/read/28346726/predictive-value-of-p63-ki-67-and-survivin-expression-in-oral-leukoplakia-a-tissue-microarray-study
#1
Kelly Bienk Dias, Anacláudia Pereira Costa Flores, Márcia Gaiger Oliveira, Pantelis Varvaki Rados, Manoel Sant'ana Filho
The aim of this study was to analyze the immunohistochemical expression of survivin, ki-67, and p63 in oral leukoplakic lesions, histopathologically differentiated into dysplastic and nondysplastic. A tissue microarray containing 57 samples of biopsies from clinically classified lesions, such as leukoplakia, was immunolabeled for survivin, ki-67, and p63. Samples were scored for percentage of positively stained. Scores were designated as follows: low = less than 25% of positive cells; and high = more than 25% of positive cells...
March 27, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28345870/ultra-fast-separation-and-analysis-of-monoclonal-antibody-aggregates-using-membrane-chromatography
#2
Pedram Madadkar, Umatheny Umatheva, Geoff Hale, Yves Durocher, Raja Ghosh
We discuss a method for rapid and cost-effective analysis of monoclonal antibody (mAb) aggregates. Hydrophobic interaction membrane chromatography, which was previously shown to be highly suitable for such separation and analysis, was used in a recently developed format referred to as laterally-fed membrane chromatography (or LFMC). A stack of rectangular polyvinylidene fluoride (or PVDF) membranes having 0.22 µm pores housed within a modified analytical-scale LFMC device was used for analyzing aggregate types and content in different monoclonal antibody samples...
March 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28345691/surface-plasmon-resonance-imaging-for-abh-antigen-detection-on-red-blood-cells-and-in-saliva-secretor-status-related-abo-subgroup-identification
#3
Patjaree Peungthum, Krisda Sudprasert, Ratthasart Amarit, Armote Somboonkaew, Boonsong Sutapun, Apirom Vongsakulyanon, Wuttigrai Seedacoon, Pimpun Kitpoka, Mongkol Kunakorn, Toemsak Srikhirin
Low antigenic expression of ABO subgroup system on red blood cell (RBC) is cause of discrepancy between forward and reverse blood typing in the standard agglutination technique. Neutralization agglutination is employed for verification of the detection of ABH substances in saliva. However, the neutralization technique is complicated, time-consuming and requires expertise. To overcome these drawbacks, surface plasmon resonance (SPR) imaging was developed for ABH antigen detection on RBCs and in saliva. An antibody array was designed to classify the ABO subgroups by anti-A, anti-B, and anti-H antibodies; the array was immobilized on a carboxymethyl-dextran sensor-surface...
March 27, 2017: Analyst
https://www.readbyqxmd.com/read/28345657/multicolor-bioimaging-with-biosynthetic-zinc-nanoparticles-and-their-application-in-tumor-detection
#4
Yanjun Kang, Yi-Zhou Wu, Xianyun Hu, Xueqin Xu, Jie Sun, Rong Geng, Tongxing Huang, Xiaohang Liu, Yichen Ma, Ying Chen, Quan Wan, Xiaobang Qi, Gen Zhang, Xiaohui Zhao, Xin Zeng
Because they generate excellent images, nanoparticles (NPs), especially biosynthesized NPs, provide a new solution for tumor imaging. In this research, we unveil a novel type of biosynthesized NPs featuring multicolor fluorescence. These NPs exhibit little cytotoxicity to cells. The explored NPs, designated Zn-ZFP-GST NPs (Zinc NPs in abbreviation), are generated from leukemia cells treated with a Zn(2+) solution, while zinc-finger protein and glutathione S-transferase (GST) were also identified in the Zinc NPs...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28345261/antibodies-from-plants-for-bionanomaterials
#5
REVIEW
Gueven Edgue, Richard M Twyman, Veronique Beiss, Rainer Fischer, Markus Sack
Antibodies are produced as part of the vertebrate adaptive immune response and are not naturally made by plants. However, antibody DNA sequences can be introduced into plants, and together with laboratory technologies that allow the design of antibodies recognizing any conceivable molecular structure, plants can be used as 'green factories' to produce any antibody at all. The advent of plant-based transient expression systems in particular allows the rapid, convenient, and safe production of antibodies, ranging from laboratory-scale expression to industrial-scale manufacturing...
March 27, 2017: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
https://www.readbyqxmd.com/read/28344808/safety-tumor-trafficking-and-immunogenicity-of-chimeric-antigen-receptor-car-t-cells-specific-for-tag-72-in-colorectal-cancer
#6
Kristen M Hege, Emily K Bergsland, George A Fisher, John J Nemunaitis, Robert S Warren, James G McArthur, Andy A Lin, Jeffrey Schlom, Carl H June, Stephen A Sherwin
BACKGROUND: T cells engineered to express chimeric antigen receptors (CARs) have established efficacy in the treatment of B-cell malignancies, but their relevance in solid tumors remains undefined. Here we report results of the first human trials of CAR-T cells in the treatment of solid tumors performed in the 1990s. METHODS: Patients with metastatic colorectal cancer (CRC) were treated in two phase 1 trials with first-generation retroviral transduced CAR-T cells targeting tumor-associated glycoprotein (TAG)-72 and including a CD3-zeta intracellular signaling domain (CART72 cells)...
2017: Journal for Immunotherapy of Cancer
https://www.readbyqxmd.com/read/28344580/a-perspective-on-the-development-of-plant-made-vaccines-in-the-fight-against-ebola-virus
#7
REVIEW
Sergio Rosales-Mendoza, Ricardo Nieto-Gómez, Carlos Angulo
The Ebola virus (EBOV) epidemic indicated a great need for prophylactic and therapeutic strategies. The use of plants for the production of biopharmaceuticals is a concept being adopted by the pharmaceutical industry, with an enzyme for human use currently commercialized since 2012 and some plant-based vaccines close to being commercialized. Although plant-based antibodies against EBOV are under clinical evaluation, the development of plant-based vaccines against EBOV essentially remains an unexplored area...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28344412/clinical-and-hematobiochemical-response-in-canine-monocytic-ehrlichiosis-seropositive-dogs-of-punjab
#8
Manasa R Kottadamane, Pritpal Singh Dhaliwal, Lachhman Das Singla, Baljinder Kumar Bansal, Sanjeev Kumar Uppal
AIM: As in India especially, the Punjab state sero-prevalence and distribution of ehrlichiosis in relation to clinico-hematobiochemical response remains largely unexplored. Thus, this study was designed to determine the prevalence of vector (tick)-borne tropical canine pancytopenia caused by Ehrlichia canis through enzyme labeled ImmunoComb(®) (IC) assay in dogs from in and around Ludhiana, Punjab. Correlation of prevalence was made with various clinico-hematobiochemical parameters. MATERIALS AND METHODS: Seroprevalence study was carried out using IC(®) test kit (Biogal, Galed Labs)...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28343776/a-effective-dna-vaccine-against-diverse-genotype-j-infectious-hematopoietic-necrosis-virus-strains-prevalent-in-china
#9
Liming Xu, Jingzhuang Zhao, Miao Liu, Gael Kurath, Guangming Ren, Scott E Lapatra, Jiasheng Yin, Hongbai Liu, Jian Feng, Tongyan Lu
Infectious hematopoietic necrosis virus (IHNV) is the most important pathogen threatening the aquaculture of salmonid fish in China. In this study, a DNA vaccine, designated pIHNch-G, was constructed with the glycoprotein (G) gene of a Chinese IHNV isolate SD-12 (also called Sn1203) of genotype J. The minimal dose of vaccine required, the expression of the Mx-1 gene in the muscle (vaccine delivery site) and anterior kidney, and the titers of the neutralizing antibodies produced were used to evaluate the vaccine efficacy...
March 23, 2017: Vaccine
https://www.readbyqxmd.com/read/28343671/cytotoxicity-and-proinflammatory-cytokine-expression-induced%C3%A2-by-interim-resin-materials-in-primary-cultured-human%C3%A2-dental-pulp-cells
#10
Jung-Hwan Lee, Soo-Kyung Jun, Ho-Jin Moon, Hae-Hyoung Lee
STATEMENT OF PROBLEM: Acrylic resin materials for interim restoration may adversely affect pulp tissue during the polymerization phase. PURPOSE: The purpose of this in vitro study was to determine the cytotoxic and proinflammatory cytokine production effects induced by interim resin materials in primary cultured human dental pulp cells (hDPCs). MATERIAL AND METHODS: Five interim resin materials were evaluated: 3 types of chemically activated products, 1 light-activated product, and 1 computer-aided design and computer-aided manufacturing (CAD-CAM) product...
March 23, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28343398/an-anti-programmed-death-1-antibody-a%C3%AF-pd-1-fusion-protein-that-self-assembles-into-a-multivalent-and-functional-apd-1-nanoparticle
#11
Peng Zhao, Djordje Atanackovic, Shuyun Dong, Hideo Yagita, Xiao He, Mingnan Chen
Cancer immune checkpoint therapy has achieved remarkable clinical successes in various cancers. However, current immune checkpoint inhibitors block the checkpoint of not only the immune cells that are important to cancer therapy but also the immune cells that are irrelevant to the therapy. Such an indiscriminate blockade limits the efficacy and causes the autoimmune toxicity of the therapy. It might be beneficial to use a carrier to target immune checkpoint inhibitors to cancer-reactive immune cells. Here, we explore a method to load the inhibitors into carriers...
March 25, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28342851/site-specific-photocoupling-of-pbpa-mutated-scfv-antibodies-for-use-in-affinity-proteomics
#12
Mattias Brofelth, Lars Wagner Städe, Anna Isinger Ekstrand, Linn Petersson Edfeldt, Rebeka Kovačič, Thorbjørn Terndrup Nielsen, Kim Lambertsen Larsen, Laurent Duroux, Christer Wingren
Recombinant antibody libraries can provide a source of renewable and high-performing binders tailored for use in affinity proteomics. In this context, the process of generating site-specific 1:1 tagging/functionalization and/or orientated surface immobilization of antibodies has, however, proved to be challenging. Hence, novel ways of generating such engineered antibodies for use in affinity proteomics could have a major impact on array performance. In this study, we have further tailored the design of human recombinant scFv antibodies for site-specific photocoupling through the use of an unnatural amino acid (UAA) and the Dock'n'Flash technology...
March 22, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28341111/generation-characterization-and-immunogenicity-of-a-novel-chimeric-recombinant-protein-based-on-plasmodium-vivax-ama-1-and-msp119
#13
Mariana Vilela Rocha, Kátia Sanches Françoso, Luciana Chagas Lima, Tarsila Mendes Camargo, Ricardo L D Machado, Fabio T M Costa, Laurent Rénia, Francois Nosten, Bruce Russell, Mauricio M Rodrigues, Irene S Soares
Plasmodium vivax is the most widely distributed malaria species and the most prevalent species of malaria in America and Asia. Vaccine development against P. vivax is considered a priority in the global program for the eradication of malaria. Earlier studies have characterized the Apical Membrane Antigen 1 (AMA-1) ectodomain and the C-terminal region (19kDa) of the Merozoite Surface Protein 1 (MSP-1) of P. vivax as immunodominant antigens. Based on this characterization, we designed a chimeric recombinant protein containing both merozoite immunodominant domains (PvAMA166-MSP119)...
March 21, 2017: Vaccine
https://www.readbyqxmd.com/read/28340580/toll-like-receptors-genes-polymorphisms-and-the-occurrence-of-hcmv-infection-among-pregnant-women
#14
Wioletta Wujcicka, Edyta Paradowska, Mirosława Studzińska, Jan Wilczyński, Dorota Nowakowska
BACKGROUND: Human cytomegalovirus (HCMV) is the most common cause of intrauterine infections worldwide. The toll-like receptors (TLRs) have been reported as important factors in immune response against HCMV. Particularly, TLR2, TLR4 and TLR9 have been shown to be involved in antiviral immunity. Evaluation of the role of single nucleotide polymorphisms (SNPs), located within TLR2, TLR4 and TLR9 genes, in the development of human cytomegalovirus (HCMV) infection in pregnant women and their fetuses and neonates, was performed...
March 24, 2017: Virology Journal
https://www.readbyqxmd.com/read/28339909/il-17-and-cd40-ligand-synergistically-stimulate-the-chronicity-of-diabetic-nephropathy
#15
Huey-Liang Kuo, Chiu-Ching Huang, Tze-Yi Lin, Ching-Yuang Lin
Background.: Early stages of diabetic nephropathy (DN) are characterized by an influx of inflammatory cells. Interactions between infiltrating T cells and podocytes may play an important role in the ongoing inflammatory response and remodelling. The aim of this study was to explore the role of IL-17 and CD40 ligand (CD40L) in DN. Methods.: The study design involved a case series. Kidney biopsy samples of 69 patients with type 2 diabetes were assessed for the presence of CD4 +  IL-17 +  T cells...
March 1, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28338065/prospects-for-combining-targeted-and-conventional-cancer-therapy-with-immunotherapy
#16
REVIEW
Philip Gotwals, Scott Cameron, Daniela Cipolletta, Viviana Cremasco, Adam Crystal, Becker Hewes, Britta Mueller, Sonia Quaratino, Catherine Sabatos-Peyton, Lilli Petruzzelli, Jeffrey A Engelman, Glenn Dranoff
Over the past 25 years, research in cancer therapeutics has largely focused on two distinct lines of enquiry. In one approach, efforts to understand the underlying cell-autonomous, genetic drivers of tumorigenesis have led to the development of clinically important targeted agents that result in profound, but often not durable, tumour responses in genetically defined patient populations. In the second parallel approach, exploration of the mechanisms of protective tumour immunity has provided several therapeutic strategies - most notably the 'immune checkpoint' antibodies that reverse the negative regulators of T cell function - that accomplish durable clinical responses in subsets of patients with various tumour types...
March 24, 2017: Nature Reviews. Cancer
https://www.readbyqxmd.com/read/28337237/relationship-between-helicobacter-pylori-infection-and-celiac-disease-a-cross-sectional-study-and-a-brief-review-of-the-literature
#17
Sebahat Basyigit, Oktay Unsal, Metin Uzman, Ferdane Sapmaz, Ozlem Ceylan Dogan, Ayse Kefeli, Zeliha Asilturk, Abdullah Ozgur Yeniova, Yasar Nazligul
INTRODUCTION: Whether Helicobacter pylori triggers celiac disease (CD) or protects against CD is currently the subject of research. In the literature, there are epidemiologic studies that have reported conflicting results regarding the association between H. pylori and CD. AIM: To compare the prevalence of CD autoantibody positivity and the levels of CD autoantibodies between H. pylori-positive and H. pylori-negative subjects. MATERIAL AND METHODS: This study was prospectively designed and included 240 dyspeptic patients who underwent upper gastrointestinal endoscopy with gastric and duodenal biopsies...
2017: Przegla̜d Gastroenterologiczny
https://www.readbyqxmd.com/read/28337197/hypofractionated-irradiation-has-immune-stimulatory-potential-and-induces-a-timely-restricted-infiltration-of-immune-cells-in-colon-cancer-tumors
#18
Benjamin Frey, Michael Rückert, Julia Weber, Xaver Mayr, Anja Derer, Michael Lotter, Christoph Bert, Franz Rödel, Rainer Fietkau, Udo S Gaipl
In addition to locally controlling the tumor, hypofractionated radiotherapy (RT) particularly aims to activate immune cells in the RT-modified microenvironment. Therefore, we examined whether hypofractionated RT can activate dendritic cells (DCs), induce immune cell infiltration in tumors, and how the chronology of immune cell migration into tumors occurs to gain knowledge for future definition of radiation breaks and inclusion of immunotherapy. Colorectal cancer treatments offer only limited survival benefit, and immunobiological principles for additional therapies need to be explored with preclinical models...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28336309/usefulness-of-the-ellipro-epitope-predictor-program-in-defining-the-repertoire-of-hla-abc-eplets
#19
Rene J Duquesnoy, Marilyn Marrari
HLA matching at the epitope level offers new opportunities to identify suitable donors for transplant patients. The International HLA Epitope Registry (www.Epregistry.com.br) describes for the various HLA loci, repertoires of eplets including those that correspond to epitopes experimentally verified with specific antibodies. There are also many eplets which have remained as theoretical entities because no informative antibodies have been found. Which of them have immunogenic potential or conversely, might be considered as non-epitopes that cannot elicit specific antibody responses? This question is important for the application of epitope-based HLA matching in clinical transplantation...
March 20, 2017: Human Immunology
https://www.readbyqxmd.com/read/28332900/fc-or-not-fc-that-is-the-question-antibody-fc-receptor-interactions-are-key-to-universal-influenza-vaccine-design
#20
Sinthujan Jegaskanda, Hillary A Vanderven, Adam K Wheatley, Stephen J Kent
A universal vaccine that provides long-lasting protection from both epidemic and pandemic influenza viruses remains the "holy grail" of influenza vaccine research. Though virus neutralization assays are the current benchmark of measuring vaccine effectiveness, it is clear that Fc-receptor functions can drastically improve the effectiveness of antibodies and vaccines in vivo. Antibodies that kill virus-infected cells and/or elicit an antiviral environment, termed antibody-dependent cellular cytotoxicity (ADCC)-mediating antibodies, provide a link between the innate and adaptive immune response...
March 23, 2017: Human Vaccines & Immunotherapeutics
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