keyword
MENU ▼
Read by QxMD icon Read
search

stem cell, injury, regeneration

keyword
https://www.readbyqxmd.com/read/28544974/cetuximab-modified-collagen-scaffold-directs-neurogenesis-of-injury-activated-endogenous-neural-stem-cells-for-acute-spinal-cord-injury-repair
#1
Xing Li, Yannan Zhao, Shixiang Cheng, Sufang Han, Muya Shu, Bing Chen, Xuyi Chen, Fengwu Tang, Nuo Wang, Yue Tu, Bin Wang, Zhifeng Xiao, Sai Zhang, Jianwu Dai
Studies have shown that endogenous neural stem cells (NSCs) activated by spinal cord injury (SCI) primarily generate astrocytes to form glial scar. The NSCs do not differentiate into neurons because of the adverse microenvironment. In this study, we defined the activation timeline of endogenous NSCs in rats with severe SCI. These injury-activated NSCs then migrated into the lesion site. Cetuximab, an EGFR signaling antagonist, significantly increased neurogenesis in the lesion site. Meanwhile, implanting cetuximab modified linear ordered collagen scaffolds (LOCS) into SCI lesion sites in dogs resulted in neuronal regeneration, including neuronal differentiation, maturation, myelination, and synapse formation...
May 18, 2017: Biomaterials
https://www.readbyqxmd.com/read/28536315/-skeletal-muscle-stem-cell
#2
Shinsuke Yuasa
Adult skeletal muscle has its own stem cell population known as satellite cells. After muscle injury, quiescent satellite cells are activated and then proliferate and differentiate into mature skeletal muscle to ensure that muscle function is recovered. In our screen for myocyte differentiation-promoting factors, we noted markedly elevated expression of granulocyte-colony stimulating factor receptor(G-CSFR, encoded by csf3r)in the skeletal muscle developing area. Furthermore, G-CSFR was transiently expressed in regenerating myocytes of adult injured skeletal muscle, and extrinsic G-CSF supported short-term and long-term muscle regeneration in mouse model of skeletal muscle injury...
2017: Clinical Calcium
https://www.readbyqxmd.com/read/28533931/altered-expression-of-c-kit-and-nanog-in-a-rat-model-of-adriamycin-induced-chronic-heart-failure
#3
Shuo Li, Kang Guo, Junfang Wu, Zhikun Guo, Airong Li
A small number of cardiac stem cells that express the c-Kit and Nanog biomarkers can be differentiated into myocardial cells, which suggests that these stem cells may be able to repair damage and provide an internal reserve for tissue regeneration. It is unknown, however, whether the levels of myocardial stem cells are altered after heart failure (HF), and whether HF affects the myocardial regenerative ability. In this study, to address this question, we developed a rat model of chronic HF induced by Adriamycin, and examined the morphological changes in c-Kit and Nanog-expressing stem cells in heart tissue of normal and HF rats...
2017: American Journal of Cardiovascular Disease
https://www.readbyqxmd.com/read/28530131/a-novel-combination-of-a-polycaprolactone-fumarate-pclf-scaffold-with-polyethylene-terephthalate-pet-sutures-for-intra-articular-ligament-regeneration
#4
Joshua Parry, Eric R Wagner, Peter Kok, Mahrokh Dadsetan, Michael J Yaszemski, Andre J van Wijnen, Sanjeev Kakar
Intra-articular ligamentous injuries are typically unrepairable and have limited outcomes after graft reconstruction. A combination of porous polycaprolactone fumarate (PCLF) scaffolds with polyethylene terephthalate (PET) sutures was developed with the goal of regenerating intra-articular ligaments. Scaffolds were fabricated by injecting PCLF over 3D-printed molds containing two strands of PET suture down its central pore followed by crosslinking. Scaffolds were seeded with human mesenchymal stem cells (MSCs) from adipose tissue...
May 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28529106/engineered-cartilage-regeneration-from-adipose-tissue-derived-mesenchymal-stem-cells-a-morphomolecular-study-on-osteoblast-chondrocyte-and-apoptosis-evaluation
#5
Marta Anna Szychlinska, Paola Castrogiovanni, Houda Nsir, Michelino Di Rosa, Claudia Guglielmino, Rosalba Parenti, Giovanna Calabrese, Elisabetta Pricoco, Lucia Salvatorelli, Gaetano Magro, Rosa Imbesi, Ali Mobasheri, Giuseppe Musumeci
The poor self-repair capacity of cartilage tissue in degenerative conditions, such as osteoarthritis (OA), has prompted the development of a variety of therapeutic approaches, such as cellular therapies and tissue engineering based on the use of mesenchymal stem cells (MSCs). The aim of this study is to demonstrate, for the first time, that the chondrocytes differentiated from rat adipose tissue derived-MSCs (AMSCs), are able to constitute a morphologically and biochemically healthy hyaline cartilage after 6 weeks of culture on a Collagen Cell Carrier (CCC) scaffold...
May 18, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28527850/complement-activation-by-pulp-capping-materials-plays-a-significant-role-in-both-inflammatory-and-pulp-stem-cells-recruitment
#6
Thomas Giraud, Pierre Rufas, Fanny Chmilewsky, Charlotte Rombouts, Jacques Dejou, Charlotte Jeanneau, Imad About
INTRODUCTION: The role of complement, especially through the C5a fragment, is well-known for the initiation of inflammation. Its involvement in regeneration has been shown more recently by the recruitment of mesenchymal stem cells. C5a can be produced locally by the pulp fibroblasts in response to injury or infection. This work aims to investigate the effect of different pulp capping biomaterials on complement activation and its possible influence on inflammatory and pulp stem cell recruitment...
May 17, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28527111/human-placenta-derived-mesenchymal-stem-cells-loaded-on-linear-ordered-collagen-scaffold-improves-functional-recovery-after-completely-transected-spinal-cord-injury-in-canine
#7
Sufang Han, Zhifeng Xiao, Xing Li, Huan Zhao, Bin Wang, Zhixue Qiu, Zhi Li, Xin Mei, Bai Xu, Caixia Fan, Bing Chen, Jin Han, Yanzheng Gu, Huilin Yang, Qin Shi, Jianwu Dai
Traumatic spinal cord injury (SCI) is a major challenge in the clinic. In this study, we sought to examine the synergistic effects of linear ordered collagen scaffold (LOCS) and human placenta-derived mesenchymal stem cells (hPMSCs) when transplanted into completely transected beagle dogs. After 36 weeks observation, we found that LOCS+hPMSCs implants promoted better hindlimb locomotor recovery than was observed in the non-treatment (control) group and LOCS group. Histological analysis showed that the regenerated tissue after treatment was well integrated with the host tissue, and dramatically reduced the volume of cystic and chondroitin sulfate proteoglycans (CSPGs) expression...
May 16, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28520221/rap1b-is-an-effector-of-axin2-regulating-crosstalk-of-signaling-pathways-during-skeletal-development
#8
Takamitsu Maruyama, Ming Jiang, Alycia Abbott, H-M Ivy Yu, Qirong Huang, Magdalena Chrzanowska-Wodnicka, Emily I Chen, Wei Hsu
Recent identification and isolation of suture stem cells capable of long term self-renewal, clonal expanding and differentiating demonstrate their essential role in calvarial bone development, homeostasis and injury repair. These bona fide stem cells express high level of Axin2 and are able to mediate bone regeneration and repair in a cell autonomous fashion. The importance of Axin2 is further demonstrated by its genetic inactivation in mice causing skeletal deformities resembling craniosynostosis in humans...
May 18, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28514329/-brain-plasticity-limitations-and-possibilities
#9
E P Kharchenko, M N Telnova
Biological limitations related to the brain regeneration and stem cells transplantation as well as the factors influencing the brain plasticity following the brain injury, including epigenetic regulatory mechanisms, are presented. Non-invasive transcranial microelectrostimulation is considered as a perspective method of polysystemic influence on endogenic mechanisms of brain recovery.
2017: Zhurnal Nevrologii i Psikhiatrii Imeni S.S. Korsakova
https://www.readbyqxmd.com/read/28506533/schwann-cell-precursors-from-human-pluripotent-stem-cells-as-a-potential-therapeutic-target-for-myelin-repair
#10
Han-Seop Kim, Jungwoon Lee, Da Yong Lee, Young-Dae Kim, Jae Yun Kim, Hyung Jin Lim, Sungmin Lim, Yee Sook Cho
Schwann cells play a crucial role in successful nerve repair and regeneration by supporting both axonal growth and myelination. However, the sources of human Schwann cells are limited both for studies of Schwann cell development and biology and for the development of treatments for Schwann cell-associated diseases. Here, we provide a rapid and scalable method to produce self-renewing Schwann cell precursors (SCPs) from human pluripotent stem cells (hPSCs), using combined sequential treatment with inhibitors of the TGF-β and GSK-3 signaling pathways, and with neuregulin-1 for 18 days under chemically defined conditions...
May 11, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28505619/bone-marrow-mesenchymal-stem-cell-transplantation-increases-gap-43-expression-via-erk1-2-and-pi3k-akt-pathways-in-intracerebral-hemorrhage
#11
Jianzhong Cui, Changmeng Cui, Ying Cui, Ran Li, Huaxin Sheng, Xiaohua Jiang, Yanxia Tian, Kaijie Wang, Junling Gao
BACKGROUND/AIMS: Intracerebral hemorrhage (ICH) occurs in hypertensive patients and results in high rates of mortality and disability. This study determined whether bone marrow mesenchymal stem cell (BMSC) transplantation affects axonal regeneration and examined the underlying mechanisms after the administration of PD98059 (p-ERK1/2 inhibitor) or/ and LY294002 (PI3K inhibitor). The hypothesis that was intended to be tested was that BMSC transplantation regulates the expression of growth-associated protein-43 (GAP-43) via the ERK1/2 and PI3K/Akt signaling pathways...
May 12, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28501713/an-in-situ-photocrosslinkable-platelet-rich-plasma-complexed-hydrogel-glue-with-growth-factor-controlled-release-ability-to-promote-cartilage-defect-repair
#12
Xiaolin Liu, Yunlong Yang, Xin Niu, Qiuning Lin, Bizeng Zhao, Yang Wang, Linyong Zhu
The repair of articular cartilage injury is a great clinical challenge. Platelet-rich plasma (PRP) has attracted much attention for the repair of articular cartilage injury, because it contains various growth factors that are beneficial for wound repair. However, current administration methods of PRP face many shortcomings, such as unstable biological fixation and burst release of growth factors, all of which will bring troubles to its application in the repair of articular cartilage and compromise its therapeutic efficacy...
May 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28501476/endothelial-cells-are-not-required-for-specification-of-respiratory-progenitors
#13
Jamie A Havrilak, Kristin R Melton, John M Shannon
Crosstalk between mesenchymal and epithelial cells influences organogenesis in multiple tissues, such as lung, pancreas, liver, and the nervous system. Lung mesenchyme comprises multiple cell types, however, and precise identification of the mesenchymal cell type(s) that drives early events in lung development remains unknown. Endothelial cells have been shown to be required for some aspects of lung epithelial patterning, lung stem cell differentiation, and regeneration after injury. Furthermore, endothelial cells are involved in early liver and pancreas development...
May 10, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28495756/cystic-fibrosis-transmembrane-conductance-regulator-mediates-tenogenic-differentiation-of-tendon-derived-stem-cells-and-tendon-repair-accelerating-tendon-injury-healing-by-intervening-in-its-downstream-signaling
#14
Yang Liu, Jia Xu, Liangliang Xu, Tianyi Wu, Yuxin Sun, Yuk-Wai Lee, Bin Wang, Hsiao-Chang Chan, Xiaohua Jiang, Jinfang Zhang, Gang Li
Tendons are a mechanosensitive tissue, which enables them to transmit to bone forces that are derived from muscle. Patients with tendon injuries, such as tendinopathy or tendon rupture, were often observed with matrix degeneration, and the healing of tendon injuries remains a challenge as a result of the limited understanding of tendon biology. Our study demonstrates that the stretch-mediated activation channel, cystic fibrosis transmembrane conductance regulator (CFTR), was up-regulated in tendon-derived stem cells (TDSCs) during tenogenic differentiation under mechanical stretching...
May 11, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28484495/mitochondrial-dna-hypomethylation-is-a-biomarker-associated-with-induced-senescence-in-human-fetal-heart-mesenchymal-stem-cells
#15
Dehai Yu, Zhonghua Du, Lingling Pian, Tao Li, Xue Wen, Wei Li, Su-Jeong Kim, Jialin Xiao, Pinchas Cohen, Jiuwei Cui, Andrew R Hoffman, Ji-Fan Hu
Background. Fetal heart can regenerate to restore its normal anatomy and function in response to injury, but this regenerative capacity is lost within the first week of postnatal life. Although the specific molecular mechanisms remain to be defined, it is presumed that aging of cardiac stem or progenitor cells may contribute to the loss of regenerative potential. Methods. To study this aging-related dysfunction, we cultured mesenchymal stem cells (MSCs) from human fetal heart tissues. Senescence was induced by exposing cells to chronic oxidative stress/low serum...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28483644/human-mesenchymal-stem-cells-secrete-hyaluronan-coated-extracellular-vesicles
#16
Uma Thanigai Arasu, Riikka Kärnä, Kai Härkönen, Sanna Oikari, Arto Koistinen, Heikki Kröger, Chengjuan Qu, Mikko J Lammi, Kirsi Rilla
Extracellular vesicles (EVs) secreted by stem cells are potential factors mediating tissue regeneration. They travel from bone marrow stem cells into damaged tissues, suggesting that they can repair tissue injuries without directly replacing parenchymal cells. We have discovered that hyaluronan (HA) synthesis is associated with the shedding of HA-coated EVs. The aim of this study was to test whether bone marrow-derived hMSCs secrete HA-coated EVs. The EVs secreted by MSCs were isolated by differential centrifugation and characterized by nanoparticle tracking analysis...
May 5, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28482145/a-controlled-release-system-for-simultaneous-delivery-of-three-human-perivascular-stem-cell-derived-factors-for-tissue-repair-and-regeneration
#17
Nurlan Mansurov, William C W Chen, Hassan Awada, Johnny Huard, Yadong Wang, Arman Saparov
Transplanted stem/progenitor cells improve tissue healing and regeneration anatomically and functionally mostly due to their secreted trophic factors. However, harsh conditions at the site of injury, including hypoxia, oxidative and inflammatory stress, increased fibrosis and insufficient angiogenesis, and in some cases immunological response or incompatibility, are detrimental to stem cell survival. To overcome the complexity and deficiencies of stem cell therapy, the coacervate delivery platform is deemed promising because it offers controlled and sustained release using heparin to recapitulate the binding and stabilization of extracellular proteins by heparan sulfates in native tissues...
May 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28482124/collagen-scaffold-combined-with-human-umbilical-cord-derived-mesenchymal-stem-cells-promote-functional-recovery-after-scar-resection-in-rats-with-chronic-spinal-cord-injury
#18
Nuo Wang, Zhifeng Xiao, Yannan Zhao, Bin Wang, Xing Li, Jing Li, Jianwu Dai
Effective therapeutic strategies for treating chronic spinal cord injury (SCI) are currently unavailable. Scar tissue in the lesion area is a main inhibitory factor for axonal regeneration and repair of chronic SCI. In this study, scar tissue was surgically resected from adult rats with 12-week chronic SCI, and then collagen scaffold (named NeuroRegen Scaffold [NRS]) and human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were implanted into the resected cavity to repair chronic SCI. The results demonstrated that the locomotor function of rats was not affected by surgical scar resection, indicating its safety in treating chronic SCI...
May 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28481227/long-lived-keratin-15-esophageal-progenitor-cells-contribute-to-homeostasis-and-regeneration
#19
Véronique Giroux, Ashley A Lento, Mirazul Islam, Jason R Pitarresi, Akriti Kharbanda, Kathryn E Hamilton, Kelly A Whelan, Apple Long, Ben Rhoades, Qiaosi Tang, Hiroshi Nakagawa, Christopher J Lengner, Adam J Bass, E Paul Wileyto, Andres J Klein-Szanto, Timothy C Wang, Anil K Rustgi
The esophageal lumen is lined by a stratified squamous epithelium comprised of proliferative basal cells that differentiate while migrating toward the luminal surface and eventually desquamate. Rapid epithelial renewal occurs, but the specific cell of origin that supports this high proliferative demand remains unknown. Herein, we have described a long-lived progenitor cell population in the mouse esophageal epithelium that is characterized by expression of keratin 15 (Krt15). Genetic in vivo lineage tracing revealed that the Krt15 promoter marks a long-lived basal cell population able to self-renew, proliferate, and generate differentiated cells, consistent with a progenitor/stem cell population...
May 8, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28480592/slow-myofiber-commitment-by-semaphorin-3a-secreted-from-myogenic-stem-cells
#20
Ryuichi Tatsumi, Takahiro Suzuki, Mai-Khoi Q Do, Yuki Ohya, Judy E Anderson, Ayumi Shibata, Mai Kawaguchi, Shunpei Ohya, Hideaki Ohtsubo, Wataru Mizunoya, Shoko Sawano, Yusuke Komiya, Riho Ichitsubo, Koichi Ojima, Shin-Ichiro Nishimatsu, Tsutomu Nohno, Yutaka Ohsawa, Yoshihide Sunada, Mako Nakamura, Mitsuhiro Furuse, Yoshihide Ikeuchi, Takanori Nishimura, Takeshi Yagi, Ronald E Allen
Recently, we found that resident myogenic stem satellite cells up-regulate a multi-functional secreted protein, semaphorin 3A (Sema3A), exclusively at the early- differentiation phase in response to muscle injury; however, its physiological significance is still unknown. Here we show that Sema3A impacts slow-twitch fiber generation through a signaling pathway, cell-membrane receptor (neuropilin2-plexinA3) → myogenin-myocyte enhancer factor 2D (MEF2D) → slow myosin heavy chain. This novel axis was found by small interfering RNA (siRNA)-transfection experiments in myoblast cultures, which also revealed an additional element that Sema3A-neuropilin1/plexinA1, A2 may enhance slow-fiber formation by activating signals that inhibit fast-myosin expression...
May 7, 2017: Stem Cells
keyword
keyword
67565
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"