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Philippe Lagacé-Wiens
Since the expansion of commercial use of MALDI-TOF/MS instruments for the identification of bacteria from culture which has occurred over the past 5-8 years, techniques for the identification of bacteria directly from positive blood cultures have been developed (Lagace-Wiens et al., J Clin Microbiol 50:3324-3328, 2012; Martiny et al., Eur J Clin Microbiol Infect Dis 31:2269-2281, 2012; Moussaoui et al., Clin Microbiol Infect 16:1631-1638, 2010). These techniques have the potential to provide definitive identification of pathogens causing sepsis 18-48 h earlier than conventional methodologies, and implementation of these methods has been shown to impact morbidity and hospital costs in a positive way (Martiny et al...
2015: Methods in Molecular Biology
Marios Loukas, Swetal Patel, Alper Cesmebasi, Horia Muresian, R Shane Tubbs, Diane Spicer, Marek Dabrowski
Coronary arteries have been extensively described and recognized by gross anatomic studies. However, in the clinical setting, the recognition of the conal artery is essential during coronary angiography, as well as certain congenital heart conditions such as tetralogy of Fallot. In order to provide a complete anatomic and physiologic correlation of the actual incidence and distribution of the conal artery we examined 300 formalin fixed hearts with gross dissections and 300 coronary angiograms. The conal artery was identified in all hearts examined and five main patterns were recognized...
April 2016: Clinical Anatomy
Sophie Jarraud, Ghislaine Descours, Christophe Ginevra, Gerard Lina, Jerome Etienne
Currently, several methods are used for the detection of Legionella in clinical samples, and these methods constitute part of the criteria for defining legionellosis cases. Urinary antigen detection is the first-line diagnostic test, although this test is limited to L. pneumophila serogroup 1 (Lp1) (Helbig et al., J Clin Microbiol 41:838-840, 2003). The use of molecular techniques can improve Legionaire's disease (LD) diagnosis by detecting other serogroups and species (Diederen et al., J Clin Microbiol 46:671-677, 2008)...
2013: Methods in Molecular Biology
Wei Tang, Yong-quan Shi, Jun-jie Zou, Xiang-fang Chen, Jiao-yang Zheng, Shu-wei Zhao, Zhi-min Liu
At least one in four diabetic patients is affected by peripheral neuropathy. In this study, the MALDI-TOF-MS mass spectra of peptides and proteins were generated following WCX CLINPROT bead fractionation of 39 diabetic peripheral neuropathy (DPN), 39 diabetes mellitus (DM), and 35 control (CON) serum samples. The spectra were analyzed statistically using flexAnalysisTM and Clin-ProtTM bioinformatics software. Identification of the selected markers was performed and affinity bead-purified plasma protein was subjected to LTQ Orbitrap XL MS/MS analysis followed by Mascot identification of the peptide sequences...
2011: Frontiers in Bioscience (Landmark Edition)
Chang-yu Li, Xiao-lin Wang, Jian-hua Wang, Zhi-ping Yan, Gao-quan Gong, Jie-min Cheng, Yi Chen, Ling-xiao Liu, Guo-ping Li, Cheng-gang Wang, Dong-hua Shi
Transcatheter Arterial Chemoembolization (TACE) is the first line of treatment in inoperable hepatocellular carcinoma. Magnetic affinity beads can be used to extract peptides from un-fractionated serum samples. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) can detect the presence and the molecular mass of peptides. In this study, we used a highly optimized ClinProt-matrix-assisted laser desorption/ionization time-of flight mass spectrometer (MALDI-TOF-MS) to screen hepatocellular carcinoma markers for TACE...
2011: Frontiers in Bioscience (Elite Edition)
Wentao Liu, Xiang Gao, Qu Cai, Jianfang Li, Zhenglun Zhu, Chen Li, Xuexin Yao, Qiumeng Yang, Ming Xiang, Min Yan, Zhenggang Zhu
Early diagnosis and early treatment is known to improve prognosis for gastric cancer. Magnetic affinity beads can be used to extract peptides from un-fractionated serum samples. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) can detect the presence and the molecular mass of peptides. MALDI-TOF-MS mass spectra of peptides and proteins were generated after WCX CLINPROT bead fractionation of 62 gastric cancer serum samples. The discovery set consisted of 44 samples while the validation set was 18 serum samples...
2010: Frontiers in Bioscience (Elite Edition)
Courtney Callahan, Elisangela R Castanha, Karen F Fox, Alvin Fox
Hoffmaster et al. [Hoffmaster AR, Ravel J, Rasko DA, Chapman GD, Chute MD, Marston CK, et al. Identification of anthrax toxin genes in Bacillus cereus associated with illness resembling inhalation anthrax. Proc Natl Acad Sci U S A 2004;101:8449-54; Hoffmaster AR, Hill KK, Gee JE, Marston CK, De BK, Popovic T, et al. Characterization of Bacillus cereus isolates associated with fatal pneumonias: strains are closely related to Bacillus anthracis and harbor B. anthracis virulence genes. J Clin Microbiol 2006;44:3352-60] phylogenetically divided Bacillus cereus strains into 10 branches by amplified fragment length polymorphism (AFLP) with Branch F including all Bacillus anthracis strains and pneumonia-causing strains of B...
June 2008: Molecular and Cellular Probes
M C W Gast, J M G Bonfrer, E J van Dulken, L de Kock, E J Th Rutgers, J H M Schellens, J H Beijnen
There is an urgent need for new serum markers that can be applied in e.g. the early detection of breast cancer. Following detection of new, potential biomarkers, such as those reported by Vlahou et al. (Clin Breast Cancer 2003;4:230-239) and Laronga et al. (Dis Markers 2003;19:229-238), assessment of both their robustness and validity is essential to confirm their clinical applicability. We therefore aimed to determine robustness and validity of biomarkers reported by the authors mentioned, by analysis of an independent sample set (breast cancer: n=47, normal women: n=45) in our laboratory, according to the methods described by both authors...
2006: Cancer Biomarkers: Section A of Disease Markers
Gui-Shan Xiao, Yu-Sheng Jin, Qing-Yi Lu, Zuo-Feng Zhang, Arie Belldegrun, Robert Figlin, Allan Pantuck, Yun Yen, Frederick Li, Jianyu Rao
Using a multistep human urothelial model, we previously showed that green tea extract (GTE) selectively modulates actin remodeling in transformed cells (MC-T11), which resulted in increased cell adhesion and reduced cell motility (Lu et al., Clin Cancer Res 2005;11:1675-83). This study further analyzed which actin binding proteins (ABPs) might be involved in this process. Proteomic profiles of GTE treated and untreated MC-T11 cells using two-dimensional gel electrophoresis coupled with liquid chromatography tandem mass spectrometry (LC/MS/MS) and matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) identified 20 GTE-induced proteins...
January 1, 2007: International Journal of Cancer. Journal International du Cancer
Carole Mathelin, Anne Cromer, Corinne Wendling, Catherine Tomasetto, Marie-Christine Rio
Using surface-enhanced laser desorption/ionization-time of flight (SELDI-TOF), Li et al. [Clin Chem 48(8): 1296-1304, 2002] identified 3 serum biomarkers, BC1 (4.3 kDa), BC2 (8.1 kDa) and BC3 (8.9 kDa), whose combination significantly detects breast cancer patients from non-cancer controls. This work aimed to validate these biomarkers in an independent prospective study. We screened 89 serum samples including 49 breast cancers at pT1-4N0M0 (n = 23), pT1-4N1-3M0 (n = 17) or pT1-4N0-3M1 (n = 9) stages, 13 benign breast diseases and 27 healthy women...
March 2006: Breast Cancer Research and Treatment
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