Read by QxMD icon Read

Bone Marrow Concentrate and Osteoarthritis

A A Shetty, S J Kim, S Ahmed, S Trattnig, S A Kim, H J Jang
Introduction The morbidity and significant health economic impact associated with the chondral lesion has led to a large number of strategies for therapeutic neochondrogenesis. The challenge has been to develop techniques that are cost effective single-stage procedures with minimal surgical trauma that have undergone rigorous preclinical scrutiny and robust reproducible assessment of effectiveness. A biological repair requires the generation of a cellular and matrix composite with appropriate signalling for chondrogenic differentiation...
March 2018: Annals of the Royal College of Surgeons of England
Zhen Wang, Chenjun Zhai, Hao Fei, Junzheng Hu, Weiding Cui, Zhen Wang, Zeng Li, Weimin Fan
To evaluate the effects of intraarticular injections of autologous platelet-rich plasma (PRP) or bone marrow concentrate (BMC) on osteoarthritis (OA), 24 adult goats were equally divided into control (Ctrl), saline (NS), PRP, and BMC groups, and OA was induced by surgery in NS, PRP, and BMC groups. Autologous PRP and BMC were obtained from whole blood and bone marrow aspirates, respectively. The data revealed, platelets were increased in BMC by 1.8-fold, monocytes by 5.6-fold, TGF-β1 by 7.7-fold and IGF-1 by 3...
February 21, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Elisabeth R Garwood, Christopher J Burke, Laith M Jazrawi, Ronald S Adler
Emerging musculoskeletal applications for local administration of autologous bone marrow aspirate concentrate (BMAC) include treatment of fractures, osteonecrosis, osteochondral injuries, osteoarthritis, ligament injury, tendon injury, and tendonopathies. Ultrasound-guided technique for various BMAC injection sites is detailed in this technical report and our preliminary clinical experience outlined.Five patients, 1 woman and 4 men, were treated with 6 peri/intratendinous (n = 4) or intraarticular (n = 2) BMAC injections between July 5, 2015 and December 31, 2016 for the clinical indications of common hamstrings origin tendinosis (n = 4), hip labral tear (n = 1), and osteochondral lesion of the talus (n = 1)...
January 23, 2018: Ultrasound Quarterly
Jorge Chahla, Nicholas I Kennedy, Andrew G Geeslin, Gilbert Moatshe, Mark E Cinque, Nicholas N DePhillipo, Robert F LaPrade
Meniscal injuries and meniscal loss are associated with changes in knee kinematics and loading, ultimately leading to poor functional outcomes and increased risk of progression to osteoarthritis. Biomechanical studies have shown restored knee function, and clinical studies have reported improved outcomes and decreased risk of osteoarthritis after meniscal repair. This has led orthopaedic surgeons to try and save the meniscus by repair whenever possible, as shown by increasing incidence of meniscal repair surgeries...
December 2017: Arthroscopy Techniques
Alina C Stout, Mary F Barbe, Charles B Eaton, Mamta Amin, Fatimah Al-Eid, Lori Lyn Price, Bing Lu, Grace H Lo, Ming Zhang, Jincheng Pang, Timothy E McAlindon, Jeffrey B Driban
BACKGROUND: Greater age and body mass index are strong risk factors for osteoarthritis (OA). Older and overweight individuals may be more susceptible to OA because these factors alter tissue turnover in menisci, articular cartilage, and bone via altered glucose homeostasis and inflammation. Understanding the role of inflammation and glucose homeostasis on structural features of early-stage OA may help identify therapeutic targets to delay or prevent the onset of OA among subsets of adults with these features...
January 5, 2018: BMC Musculoskeletal Disorders
Jiehua Wu, Susan Shimmon, Sharon Paton, Christopher Daly, Tony Goldschlager, Stan Gronthos, Andrew C W Zannettino, Peter Ghosh
BACKGROUND: The pharmaceutical agent pentosan polysulfate (PPS) is known to induce proliferation and chondrogenesis of mesenchymal progenitor cells (MPCs) in vitro and in vivo. However, the mechanism(s) of action of PPS in mediating these effects remains unresolved. In the present report we address this issue by investigating the binding and uptake of PPS by MPCs and monitoring gene expression and proteoglycan biosynthesis before and after the cells had been exposed to limited concentrations of PPS and then re-established in culture in the absence of the drug (MPC priming)...
December 13, 2017: Stem Cell Research & Therapy
Ewa Kuca-Warnawin, Weronika Kurowska, Monika Prochorec-Sobieszek, Anna Radzikowska, Tomasz Burakowski, Urszula Skalska, Magdalena Massalska, Magdalena Plebańczyk, Barbara Małdyk-Nowakowska, Iwona Słowińska, Robert Gasik, Włodzimierz Maśliński
BACKGROUND: Rheumatoid arthritis (RA) is a systemic, autoimmune disease leading to joint destruction and ultimately disability. Bone marrow (BM) is an important compartment in RA, where pathological processes from "outside the joint" can occur. IL-17 is a cytokine that exerts proinflammatory effects and participates in the process of bone destruction. It is believed that IL-17 is involved in pathogenesis of RA. However, little is known about the biology of this cytokine in BM...
December 8, 2017: Arthritis Research & Therapy
Marwa Qadri, Sara Almadani, Gregory D Jay, Khaled A Elsaid
Osteoarthritis (OA) is a low-grade chronic inflammatory joint disease. Innate immunity contributes to OA progression, mediated by TLR2 and TLR4. We evaluated the role of cluster determinant 44 (CD44), a transmembrane glycoprotein, in regulating TLR2-linked macrophage activation and resultant proinflammatory responses. TLR2 stimulation was performed on differentiated THP-1 macrophages in the presence or absence of a CD44-specific Ab or hyaluronan (HA). NF-κB nuclear translocation, IL-1 β and TNF-α gene expression, and protein concentrations were determined...
January 15, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Biljana Smiljanovic, Anna Radzikowska, Ewa Kuca-Warnawin, Weronika Kurowska, Joachim R Grün, Bruno Stuhlmüller, Marc Bonin, Ursula Schulte-Wrede, Till Sörensen, Chieko Kyogoku, Anne Bruns, Sandra Hermann, Sarah Ohrndorf, Karlfried Aupperle, Marina Backhaus, Gerd R Burmester, Andreas Radbruch, Andreas Grützkau, Wlodzimierz Maslinski, Thomas Häupl
OBJECTIVE: Rheumatoid arthritis (RA) accompanies infiltration and activation of monocytes in inflamed joints. We investigated dominant alterations of RA monocytes in bone marrow (BM), blood and inflamed joints. METHODS: CD14+ cells from BM and peripheral blood (PB) of patients with RA and osteoarthritis (OA) were profiled with GeneChip microarrays. Detailed functional analysis was performed with reference transcriptomes of BM precursors, monocyte blood subsets, monocyte activation and mobilisation...
February 2018: Annals of the Rheumatic Diseases
Zhao Zhang, Xiaofei Li, Heng Huang, Guozhong Wang, Zhigang Qu, Haining Zhang
BACKGROUND This study aimed to observe the effect of lentivirus-mediated cyclooxygenase-2 and aggrecanase-1 silencing and insulin-like growth factor-1 overexpression in human bone marrow mesenchymal stem cells after injection into model osteoarthritic knees. MATERIAL AND METHODS Using genetic recombination technique, the genes of cyclooxygenase-2, aggrecanase-1, and insulin-like growth factor-1 were recombined into the lentiviral vectors, and we transfected the human bone marrow stem cells in vitro. The BMSC transfected with lentivirus without genes served as a blank-virus group, and saline was used for another control group...
November 7, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Giovanna Desando, Isabella Bartolotti, Carola Cavallo, Antonella Schiavinato, Cynthia Secchieri, Elizaveta Kon, Giuseppe Filardo, Maurizio Paro, Brunella Grigolo
The evaluation of key factors modulating cell homing following transplantation can provide new insights in the comprehension of unsolved biological questions about the use of cell therapies for osteoarthritis (OA). The main purpose of this in vivo study was to investigate the biodistribution of an intra-articular (IA) injection of mesenchymal stromal cells (MSCs) and bone marrow concentrate (BMC) in a rabbit OA model, and whether the additional use of sodium hyaluronate (HA) could modulate their migration and promote joint repair...
November 6, 2017: Tissue Engineering. Part C, Methods
Elizaveta Kon, Lars Engebretsen, Peter Verdonk, Stefan Nehrer, Giuseppe Filardo
BACKGROUND: Osteoarthritis (OA) is a debilitating disease resulting in substantial pain and functional limitations. A novel blood derivative has been developed to concentrate both growth factors and antagonists of inflammatory cytokines, with promising preliminary findings in terms of safety profile and clinical improvement. PURPOSE: To investigate if one intra-articular injection of autologous protein solution (APS) can reduce pain and improve function in patients affected by knee OA in a multicenter, randomized, double-blind, saline-controlled study...
January 2018: American Journal of Sports Medicine
L Rackwitz, J C Reichert, O Pullig, U Nöth
BACKGROUND: Cell and growth factor based strategies bear great potential to support the healing processes in cartilage repair and the therapy of osteoarthritic joints. OBJECTIVES: The following review provides an overview of novel experimental strategies for the therapy of focal cartilage defects and osteoarthritis, with emphasis on cell and growth factor based approaches. MATERIALS AND METHODS: The authors summarize their own data regarding the intraarticular injection of stem cells to treat osteoarthritis of the knee and provide a synopsis of the available literature discussing the most significant publications...
November 2017: Der Orthopäde
Jean-Philippe Hauzeur, Viviane De Maertelaer, Etienne Baudoux, Michel Malaise, Yves Beguin, Valérie Gangji
PURPOSE: The fracture stage of non-traumatic osteonecrosis (ON stage 3) of the femoral head (ONFH) has an unfavourable prognosis frequently requiring total hip replacement (THR). The percentage could be lowered after core decompression. In earlier non-fracture ON stages, implantation of autologous bone marrow aspirate concentrate (BMAC) improved the effect of core decompression. The purpose was to evaluate the effect of BMAC in addition to core decompression in stage 3 ONFH. METHODS: A double blind RCT was conducted comparing two groups: core decompression plus saline injection or core decompression plus BMAC implantation...
October 7, 2017: International Orthopaedics
Gilbert Moatshe, Elizabeth R Morris, Mark E Cinque, Cecilia Pascual-Garrido, Jorge Chahla, Lars Engebretsen, Robert F Laprade
- Knee pathologies including focal cartilage injuries, osteoarthritis (OA), and ligament injuries are common. The poor regeneration and healing potential of cartilage has led to the search for other treatment modalities with improved healing capacity. Furthermore, with an increasing elderly population that desires to remain active, the burden of knee pathologies is expected to increase. Increased sports participation and the desire to return to activities faster is also demanding more effective and minimally invasive treatment options...
December 2017: Acta Orthopaedica
Arianna L Gianakos, Li Sun, Jay N Patel, Donald M Adams, Frank A Liporace
AIM: To examine the evidence behind the use of concentrated bone marrow aspirate (cBMA) in cartilage, bone, and tendon repair; establish proof of concept for the use of cBMA in these biologic environments; and provide the level and quality of evidence substantiating the use of cBMA in the clinical setting. METHODS: We conducted a systematic review according to PRISMA guidelines. EMBASE, MEDLINE, and Web of Knowledge databases were screened for the use of cBMA in the repair of cartilage, bone, and tendon repair...
June 18, 2017: World Journal of Orthopedics
Karen Hambly, Somruthai Poomsalood, Emma Mundy
BACKGROUND: The purpose of this study was to assess the impact of an anti-gravity treadmill return to running programme on self-efficacy and subjective knee function following knee osteochondral surgery. CASE DESCRIPTION: A 39-year-old otherwise healthy female endurance runner with a left knee femoral cartilage grade 3-4 defect 3 cm2 . The patient underwent single step arthroscopic microfracture with Bone Marrow Aspirate Concentrate. An AlterG® anti-gravity treadmill was used to manipulate loading during a graduated phased return to running over 8 weeks...
July 2017: Physical Therapy in Sport
Darla K Moser, Mike J Schoonover, Kate M Sippel, Alix M Dieterly, Jerry W Ritchey, Corey R Wall
This report describes fibrous cyst lining injection and extracorporeal shock wave therapy (ESWT) of a medial femoral condyle (MFC) subchondral cystic lesion (SCL) resulting in catastrophic MFC fracture in an Arabian mare. The mare was presented for evaluation of a severe hind limb lameness of approximately 4 months duration. On presentation, a non-weight bearing lameness of the left hind limb with severe effusion and soft tissue swelling of the stifle region was noted. Radiographic evaluation of the stifle revealed a large SCL of the MFC with associated osteoarthritis...
2017: Open Veterinary Journal
Jorge Chahla, Sandeep Mannava, Mark E Cinque, Andrew G Geeslin, David Codina, Robert F LaPrade
Bone marrow obtained by iliac crest aspiration is a common source for harvesting mesenchymal stem cells, other progenitor cells, and associated cytokine/growth factors. Recent studies have reported good to excellent outcomes with the use of bone marrow aspirate concentrate (BMAC) for pain relief in the treatment of focal chondral lesions and osteoarthritis of the knee. However, the harvesting and processing technique are crucial to achieve satisfactory results. Several studies have examined outcomes after BMAC injection, with encouraging results, but there is a lack of consensus in terms of the frequency of injection, the amount of BMAC that is injected, and the timing of BMAC injections...
April 2017: Arthroscopy Techniques
Christopher A Smith, Tim N Board, Paul Rooney, Mark J Eagle, Stephen M Richardson, Judith A Hoyland
To improve the safe use of allograft bone, decellularization techniques may be utilized to produce acellular scaffolds. Such scaffolds should retain their innate biological and biomechanical capacity and support mesenchymal stem cell (MSC) osteogenic differentiation. However, as allograft bone is derived from a wide age-range, this study aimed to determine whether donor age impacts on the ability an osteoinductive, acellular scaffold produced from human bone to promote the osteogenic differentiation of bone marrow MSCs (BM-MSC)...
2017: PloS One
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"