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https://www.readbyqxmd.com/read/29760609/identification-of-potential-crucial-genes-and-construction-of-microrna-mrna-negative-regulatory-networks-in-osteosarcoma
#1
Yue Pan, Lingyun Lu, Junquan Chen, Yong Zhong, Zhehao Dai
Background: This study aimed to identify potential crucial genes and construction of microRNA-mRNA negative regulatory networks in osteosarcoma by comprehensive bioinformatics analysis. Methods: Data of gene expression profiles (GSE28424) and miRNA expression profiles (GSE28423) were downloaded from GEO database. The differentially expressed genes (DEGs) and miRNAs (DEMIs) were obtained by R Bioconductor packages. Functional and enrichment analyses of selected genes were performed using DAVID database...
2018: Hereditas
https://www.readbyqxmd.com/read/29704684/improved-molecular-platform-for-the-gene-therapy-of-rare-diseases-by-liver-protein-secretion
#2
REVIEW
Mickael Quiviger, Aristeidis Giannakopoulos, Sebastien Verhenne, Corinne Marie, Eleana F Stavrou, Karen Vanhoorelbeke, Zsuzsanna Izsvák, Simon F De Meyer, Aglaia Athanassiadou, Daniel Scherman
Many rare monogenic diseases are treated by protein replacement therapy, in which the missing protein is repetitively administered to the patient. However, in several cases, the missing protein is required at a high and sustained level, which renders protein therapy far from being adequate. As an alternative, a gene therapy treatment ensuring a sustained effectiveness would be particularly valuable. Liver is an optimal organ for the secretion and systemic distribution of a therapeutic transgene product. Cutting edge non-viral gene therapy tools were tested in order to produce a high and sustained level of therapeutic protein secretion by the liver using the hydrodynamic delivery technique...
April 25, 2018: European Journal of Medical Genetics
https://www.readbyqxmd.com/read/29669506/mutations-and-common-polymorphisms-in-adamts13-and-vwf-genes-responsible-for-increasing-risk-of-thrombosis
#3
Habib Haybar, Elahe Khodadi, Maria Kavianpour, Najmaldin Saki
BACKGROUND AND OBJECTIVE: ADAMTS13 (A Disintegrin-like and Metalloproteases with Thrombospondin type-1 repeats, member-13) plays an important role in vascular hemostasis by cleaving the von Willebrand Factor (vWF). ADAMTS13 and vWF are involved in the development of ischemic heart disease. In this review paper, we examine the effects of single nucleotide polymorphisms (SNPs) and mutations in the vWF and ADAMTS13 genes and their contribution to the development of thrombosis. METHODS: Relevant English-language literature were searched and retrieved from PubMed search engine (2001-2017)...
April 18, 2018: Cardiovascular & Hematological Disorders Drug Targets
https://www.readbyqxmd.com/read/29659744/human-adipose-mesenchymal-stem-cells-overexpressing-dual-chemotactic-gene-showed-enhanced-angiogenic-capacity-in-ischemic-hindlimb-model
#4
Younggeul Min, Seongho Han, Hyun Aae Ryu, Sung-Whan Kim
Aims: In present study, we sought to characterize the angio-vasculogenic property of human adipose mesenchymal stem cells (ASCs) overexpressing dual chemokine GCP-2 and SDF-1α (ASC-G/S) and to determine the therapeutic potential of ASC-G/S in the context of experimental ischemia. Methods and results: We generated ASC-G/S line and performed flow cytometry, quantitative (q)-PCR, Matrigel tube formation, Matrigel plug assays and in vivo therapeutic assays using hind limb ischemia mouse model...
April 5, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29658397/inflammation-coagulation-response-and-thrombotic-effects-induced-by-silica-nanoparticles-in-zebrafish-embryos
#5
Junchao Duan, Shuang Liang, Yang Yu, Yang Li, Lijing Wang, Zehao Wu, Yueyue Chen, Mark R Miller, Zhiwei Sun
Nowadays, nanotechnology environmental health and safety (nanoEHS) is gaining attention. We previously found that silica nanoparticles (SiNPs) could induce vascular endothelial damage. However, the subsequent toxicologic response to SiNPs-induced endothelial damage was still largely unknown. In this study, we explored the inflammation-coagulation response and thrombotic effects of SiNPs in endothelial cells and zebrafish embryos. For in vitro study, swollen mitochondria and autophagosome were observed in ultrastructural analysis...
April 14, 2018: Nanotoxicology
https://www.readbyqxmd.com/read/29618155/von-willebrand-factor-aggravates-hepatic-ischemia-reperfusion-injury-by-promoting-neutrophil-recruitment-in-mice
#6
Yasuyuki Urisono, Asuka Sakata, Hideto Matsui, Shogo Kasuda, Shiro Ono, Kiyomi Yoshimoto, Kenji Nishio, Masayuki Sho, Masashi Akiyama, Toshiyuki Miyata, Kazuo Okuchi, Satoshi Nishimura, Mitsuhiko Sugimoto
Hepatic ischaemia-reperfusion (I/R) injury is a serious liver damage that critically influences the clinical outcome of liver surgery or transplantation. Since recent studies indicated the critical involvement of von Willebrand factor (VWF) in reperfusion injuries of brain and myocardium, we hypothesized that VWF-dependent thrombotic or inflammatory responses also play a role in hepatic I/R injury. Using a mouse model of hepatic I/R injury, we explored the functional relevance of the VWF-ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13) axis in this pathologic condition...
April 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29604296/circulating-cd34-and-cd34-vegfr2-progenitor-cells-are-associated-with-klotho-kl-vs-polymorphism
#7
Maria Skrzypkowska, Bartosz Słomiński, Monika Ryba-Stanisławowska, Piotr Gutknecht, Janusz Siebert
BACKGROUND: KLOTHO is a regulator of endothelial cells activity and integrity. It has been described for the first time because of its anti-aging properties. KLOTHO encoding gene is present in many functional variants in humans, including "KL-VS" variant that has been connected with longevity and cardiovascular disease development. Few mechanisms have been proposed to explain these associations, but none of them focused on cells from CD34+ population. The aim of our study was to investigate influence of KLOTHO KL-VS polymorphism on populations of CD34+ and CD34+VEGFR2+ cells...
March 28, 2018: Microvascular Research
https://www.readbyqxmd.com/read/29587258/sulodexide-slows-down-the-senescence-of-aortic-endothelial-cells-exposed-to-serum-from-patients-with-peripheral-artery-diseases
#8
Patrycja Sosińska-Zawierucha, Beata Maćkowiak, Ryszard Staniszewski, Katarzyna Sumińska-Jasińska, Magdalena Maj, Zbigniew Krasiński, Andrzej Bręborowicz
BACKGROUND/AIMS: Aging of the arterial endothelial cells results in the appearance of their inflammatory phenotype, which may predispose patients to the acceleration of arteriosclerosis. We studied the effect of serum from patients with peripheral artery disease (PAD) on the senescence of human aortic endothelial cells (HAEC) and how that process is modulated by sulodexide. METHODS: HAEC replicative aging in vitro was studied in the presence of 10% PAD-serum (PAD Group) or10%PAD serum and Sulodexide 0...
March 10, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29571895/low-dose-cadmium-upregulates-the-expression-of-von-willebrand-factor-in-endothelial-cells
#9
Xia Wang, Fengyun Dong, Fufang Wang, Suhua Yan, Xiaocui Chen, Hideto Tozawa, Toshiyuki Ushijima, Carolyn M Kapron, Youichiro Wada, Ju Liu
Cadmium (Cd) is a persistent and widespread environmental pollutant of continuing worldwide concern. Previous studies have suggested that Cd exposure increases the risk of cardiovascular diseases, such as atherosclerosis and hypertension. However, the underlying mechanisms are poorly understood. In this study, we observed that low dose Cd treatment induced von Willebrand factor (vWF) expression in vascular endothelial cells in mouse lung and kidney tissues. In vitro analysis showed that 1 μM Cd specifically upregulated vWF mRNA and protein expression in human umbilical vein endothelial cells (HUVECs), indicating that Cd targets vascular endothelial cells even at relatively low concentrations...
March 20, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29562472/-mutation-analysis-of-vwf-gene-in-two-patients-with-von-willebrand-disease-by-target-sequence-capture-and-high-throughput-sequencing-technology
#10
Y Feng, S Y Mei, N Liu, R X Guo
No abstract text is available yet for this article.
March 14, 2018: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/29548756/ultrasound-molecular-imaging-of-atherosclerosis-using-small-peptide-targeting-ligands-against-endothelial-markers-of-inflammation-and-oxidative-stress
#11
Federico Moccetti, Craig C Weinkauf, Brian P Davidson, J Todd Belcik, Edmund R Marinelli, Evan Unger, Jonathan R Lindner
The aim of this study was to evaluate a panel of endothelium-targeted microbubble (MB) ultrasound contrast agents bearing small peptide ligands as a human-ready approach for molecular imaging of markers of high-risk atherosclerotic plaque. Small peptide ligands with established affinity for human P-selectin, VCAM-1, LOX-1 and von Willebrand factor (VWF) were conjugated to the surface of lipid-stabilized MBs. Contrast-enhanced ultrasound (CEUS) molecular imaging of the thoracic aorta was performed in wild-type and gene-targeted mice with advanced atherosclerosis (DKO)...
March 13, 2018: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/29541444/the-development-of-salt-sensitive-hypertension-regulated-by-psgl-1-gene-in-mice
#12
Yuhui Yang, Xue Liu, Yunpeng Liu, Hui Fu, Ying Gao, Xing Liu, Xiaoliang Jiang
Background and objective: Chronic inflammatory is involved in the development of salt-sensitive hypertension and other cardiovascular diseases. PSGL-1 plays an important role in the inflammatory response. Methods and results: In this study, we used PSGL-1-/- and PSGL-1+/+ mice fed with high salt diet to measure the blood pressure, inflammatory response and vascular injury. We found that, in PSGL-1+/+ mice, high salt diet resulted in high blood pressure with the increased expression of serum inflammatory cytokines IL-6, IL-1β and TNFɑ, vascular injury markers MCP-1, ET-1, and VWF, and renal macrophages and T cells infiltration, and endothelium-dependent acetylcholine vasodilation dysfunction...
2018: Cell & Bioscience
https://www.readbyqxmd.com/read/29523136/characterisation-of-feline-renal-cortical-fibroblast-cultures-and-their-transcriptional-response-to-transforming-growth-factor-%C3%AE-1
#13
J S Lawson, H M Syme, C P D Wheeler-Jones, J Elliott
BACKGROUND: Chronic kidney disease (CKD) is common in geriatric cats, and the most prevalent pathology is chronic tubulointerstitial inflammation and fibrosis. The cell type predominantly responsible for the production of extra-cellular matrix in renal fibrosis is the myofibroblast, and fibroblast to myofibroblast differentiation is probably a crucial event. The cytokine TGF-β1 is reportedly the most important regulator of myofibroblastic differentiation in other species. The aim of this study was to isolate and characterise renal fibroblasts from cadaverous kidney tissue of cats with and without CKD, and to investigate the transcriptional response to TGF-β1...
March 9, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29520365/platelet-targeted-gene-therapy-for-hemophilia
#14
REVIEW
Qizhen Shi
Gene therapy is an attractive approach for disease treatment. Since platelets are abundant cells circulating in blood with the distinctive abilities of storage and delivery and fundamental roles in hemostasis and immunity, they could be a unique target for gene therapy of diseases. Recent studies have demonstrated that ectopic expression of factor VIII (FVIII) in platelets under control of the platelet-specific promoter results in FVIII storage together with its carrier protein von Willebrand factor (VWF) in α-granules and the phenotypic correction of hemophilia A...
June 15, 2018: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/29389944/genetic-variation-in-the-c-type-lectin-receptor-clec4m-in-type-1-von-willebrand-disease-patients
#15
Eric Manderstedt, Christina Lind-Halldén, Stefan Lethagen, Christer Halldén
von Willebrand factor (VWF) levels in healthy individuals and in patients with type 1 von Willebrand disease (VWD) are influenced by genetic variation in several genes, e.g. VWF, ABO, STXBP5 and CLEC4M. This study aims to screen comprehensively for CLEC4M variants and investigate their association with type 1 VWD in the Swedish population. In order to screen for CLEC4M variants, the CLEC4M gene region was re-sequenced and the polymorphic neck region was genotyped in 106 type 1 VWD patients from unrelated type 1 VWD families...
2018: PloS One
https://www.readbyqxmd.com/read/29374149/fli1-and-pkc-co-activation-promote-highly-efficient-differentiation-of-human-embryonic-stem-cells-into-endothelial-like-cells
#16
Hao Zhao, Yan Zhao, Zili Li, Qi Ouyang, Yi Sun, Di Zhou, Pingyuan Xie, Sicong Zeng, Lingfeng Dong, Hua Wen, Guangxiu Lu, Ge Lin, Liang Hu
Rationale-endothelial cells (ECs) play important roles in various regeneration processes and can be used in a variety of therapeutic applications, such as cardiac regeneration, gene therapy, tissue-engineered vascular grafts and prevascularized tissue transplants. ECs can be acquired from pluripotent and adult stem cells. To acquire ECs from human embryonic stem cells (hESCs) in a fast, efficient and economic manner. We established a conditional overexpression system in hESCs based on 15 transcription factors reported to be responsible for hematopoiesis lineage...
January 26, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29305979/target-gene-screening-and-evaluation-of-prognostic-values-in-non-small-cell-lung-cancers-by-bioinformatics-analysis
#17
Junjie Piao, Jie Sun, Yang Yang, Tiefeng Jin, Liyan Chen, Zhenhua Lin
BACKGROUND: Non-small cell lung cancer (NSCLC) is the major leading cause of cancer-related deaths worldwide. This study aims to explore molecular mechanism of NSCLC. METHODS: Microarray dataset was obtained from the Gene Expression Omnibus (GEO) database, and analyzed by using GEO2R. Functional and pathway enrichment analysis were performed based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Then, STRING, Cytoscape and MCODE were applied to construct the Protein-protein interaction (PPI) network and screen hub genes...
March 20, 2018: Gene
https://www.readbyqxmd.com/read/29299165/gata3-induced-vwf-upregulation-in-the-lung-adenocarcinoma-vasculature
#18
Yinghua Xu, Silin Pan, Jing Liu, Fengyun Dong, Zuowang Cheng, Jinjin Zhang, Ruixia Qi, Qi Zang, Caiqing Zhang, Xia Wang, Jiandong Zhang, Fufang Wang, Thaddeus D Allen, Ju Liu
Lung adenocarcinoma (LAC) is the leading cause of cancer-related death worldwide. Aberrant expression of genes expressed preferentially in the lung tumor vasculature may yield clues for prognosis and treatment. Von Willebrand factor (vWF) is a large multifunctional glycoprotein with a well-known function in hemostasis. However, vWF has been reported to exert an anti-tumor effect, independent of its role in hemostasis. We investigated the expression of vWF in LAC through immunohistochemical staining of tumor tissue microarrays (TMAs)...
December 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/29288565/high-and-long-term-von-willebrand-factor-expression-after-sleeping-beauty-transposon-mediated-gene-therapy-in-a-mouse-model-of-severe-von-willebrand-disease
#19
I Portier, K Vanhoorelbeke, S Verhenne, I Pareyn, N Vandeputte, H Deckmyn, D S Goldenberg, H B Samal, M Singh, Z Ivics, Z Izsvák, S F De Meyer
Essentials von Willebrand disease (VWD) is the most common inherited bleeding disorder. Gene therapy for VWD offers long-term therapy for VWD patients. Transposons efficiently integrate the large von Willebrand factor (VWF) cDNA in mice. Liver-directed transposons support sustained VWF expression with suboptimal multimerization. SUMMARY: Background Type 3 von Willebrand disease (VWD) is characterized by complete absence of von Willebrand factor (VWF). Current therapy is limited to treatment with exogenous VWF/FVIII products, which only provide a short-term solution...
March 2018: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/29234431/protective-effects-of-total-flavones-of-elaeagnus-rhamnoides-l-a-nelson-against-vascular-endothelial-injury-in-blood-stasis-model-rats
#20
Zhicheng Wei, Fang Zuo, Wenqian Wang, Li Wang, Dong Tong, Yong Zeng, Ping Wang, Xianli Meng, Yi Zhang
The aim was to evaluate the protective effects of total flavones of Elaeagnus rhamnoides (L.) A. Nelson (TFE) against vascular endothelial injury in blood stasis model rats and explore the potential mechanisms preliminarily. The model of blood stasis rat model with vascular endothelial injury was induced by subcutaneous injection of adrenaline combined with ice-water bath. Whole blood viscosity (WBV), histological examination, and prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen (FIB) were measured...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
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