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Alveolar macrophage

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https://www.readbyqxmd.com/read/28822766/pseudomonas-aeruginosa-increases-muc1-expression-in-macrophages-through-the-tlr4-p38-pathway
#1
Kosuke Kato, Alec D Hanss, Marina A Zemskova, Nicole E Morgan, Marianne Kim, Kenneth S Knox, Yong Lin, Erik P Lillehoj, Kwang Chul Kim
Alveolar macrophages (AMs) play a critical role in the clearance of Pseudomonas aeruginosa (Pa) from the airways. However, hyper-activation of macrophages can impair bacterial clearance and contribute to morbidity and mortality. MUC1 mucin is a membrane-tethered, high molecular mass glycoprotein expressed on the apical surface of mucosal epithelial cells and some hematopoietic cells, including macrophages, where it counter-regulates inflammation. We recently reported that Pa up-regulates the expression of MUC1 in primary human AMs and THP-1 macrophages, and that increased MUC1 expression in these cells prevents hyper-activation of macrophages that appears to be important for host defense against severe pathology of Pa lung infection...
August 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28821221/acute-penicillium-marneffei-infection-stimulates-host-m1-m2a-macrophages-polarization-in-balb-c-mice
#2
Xiaoying Dai, Congzheng Mao, Xiuwan Lan, Huan Chen, Meihua Li, Jing Bai, Jingmin Deng, Qiuli Liang, Jianquan Zhang, Xiaoning Zhong, Yi Liang, Jiangtao Fan, Honglin Luo, Zhiyi He
BACKGROUND: Penicillium marneffei (P. marneffei) is a thermally dimorphic fungus pathogen that causes fatal infection. Alveolar macrophages are innate immune cells that have critical roles in protection against pulmonary fungal pathogens and the macrophage polarization state has the potential to be a deciding factor in disease progression or resolution. The aim of this study was to investigate mouse alveolar macrophage polarization states during P. marneffei infection. RESULTS: We used enzyme-linked immunosorbent (ELISA) assays, quantitative real-time PCR (qRT-PCR), and Griess, arginase activity to evaluate the phenotypic markers of alveolar macrophages from BALB/C mice infected with P...
August 18, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28821088/microparticles-prepared-with-50-190kda-chitosan-as-promising-non-toxic-carriers-for-pulmonary-delivery-of-isoniazid
#3
Paula M Oliveira, Breno N Matos, Priscilla A T Pereira, Taís Gratieri, Lucia H Faccioli, Marcílio S S Cunha-Filho, Guilherme M Gelfuso
Chitosan biocompatibility and mucoadhesiveness make it an ideal polymer for antituberculotic drugs microcapsulation for pulmonary delivery. Yet, previous study indicated toxicity problems to J-774.1-cells treated with some medium molecular weight (190-310kDa) chitosan microparticles. As polymer molecular weight is a crucial factor to be considered, this paper describes the preparation and characterization of chitosan (50-190kDa) microparticles containing isoniazid (INH). Cytotoxicity assays were also performed on murine peritoneal (J-774...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28819261/modified-vaccinia-virus-ankara-preferentially-targets-antigen-presenting-cells-in-vitro-ex-vivo-and-in-vivo
#4
Arwen F Altenburg, Carolien E van de Sandt, Bobby W S Li, Ronan J MacLoughlin, Ron A M Fouchier, Geert van Amerongen, Asisa Volz, Rudi W Hendriks, Rik L de Swart, Gerd Sutter, Guus F Rimmelzwaan, Rory D de Vries
Modified Vaccinia virus Ankara (MVA) is a promising vaccine vector with an excellent safety profile. However, despite extensive pre-clinical and clinical testing, surprisingly little is known about the cellular tropism of MVA, especially in relevant animal species. Here, we performed in vitro, ex vivo and in vivo experiments with recombinant MVA expressing green fluorescent protein (rMVA-GFP). In both human peripheral blood mononuclear cells and mouse lung explants, rMVA-GFP predominantly infected antigen presenting cells...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28814604/macrophage-restricted-shp2-tyrosine-phosphatase-acts-as-a-rheostat-for-mmp12-through-tgf-%C3%AE-activation-in-the-prevention-of-age-related-emphysema-in-mice
#5
Jiaqi Xu, Bo Tao, Xiaohong Guo, Shiyi Zhou, Yongda Li, Yuqin Zhang, Zanhua Zhou, Hongqiang Cheng, Xue Zhang, Yuehai Ke
Persistent activation of macrophages in lungs plays a critical role in the production of matrix metalloproteinases (MMPs) that contributes to the destruction of alveolar walls, a hallmark for pulmonary emphysema. Dysregulated TGF-β1 signaling has been an essential determinant in the elevation of MMPs during the development of emphysema. Nevertheless, the mechanism for this MMP-dependent pathogenesis has yet to be clearly investigated. Recently, we identified an important role for tyrosine phosphatase Src homology domain-containing protein tyrosine phosphatase 2 (Shp2) in regulating the activation of alveolar macrophages...
August 16, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28810254/insulin-influences-lps-induced-tnf-%C3%AE-and-il-6-release-through-distinct-pathways-in-mouse-macrophages-from-different-compartments
#6
Fernando H G Tessaro, Thais S Ayala, Eduardo L Nolasco, Leonardo M Bella, Joilson O Martins
BACKGROUND/AIMS: Diabetic subjects are more susceptible to infections, which is partially due to insulin deficiency and hyperglycemia. We hypothesized that insulin influences cytokine release by macrophages from diabetic C57BL/6 mice stimulated with lipopolysaccharides (LPS). METHODS: Bone marrow-derived macrophages (BMDM) and tissue-specific macrophages from diabetic (alloxan 60 mg/kg, i.v.) male C57BL/6 mice were stimulated by LPS (100 ng/mL) and/or treated by insulin (1 mU/mL)...
August 15, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28807225/differences-in-anti-inflammatory-effects-between-two-specifications-of-scutellariae-radix-in-lps-induced-macrophages-in-vitro
#7
Qian-Yu Chen, Chao-Qun Wang, Zhi-Wei Yang, Qi Tang, Huan-Ran Tan, Xuan Wang, Shao-Qing Cai
Scutellariae Radix (SR), the root of Scutellaria baicalensis Georgi, is used as an antipyretic drug and has been demonstrated to have anti-inflammatory activity. SR is divided into two specifications, "Ku Qin" (KQ) and "Zi Qin" (ZQ), for use against different symptoms (upper energizer heat or lower portion of the triple energizer), according to the theory of traditional Chinese medicine (TCM). However, differences in the efficacies of these two specifications have not been determined. In the present study, we aimed to characterize the differences in the anti-inflammatory activities between KQ and ZQ and to explore how their differences are manifested in lipopolysaccharide (LPS)-induced macrophages...
July 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28807099/role-of-endoplasmic-reticulum-stress-in-silica-induced-apoptosis-in-raw264-7-cells
#8
Yong Bin Hu, Xia Wu, Xiao Feng Qin, Lei Wang, Pin Hua Pan
OBJECTIVE: We investigated the role of endoplasmic reticulum stress (ERS) in silica-induced apoptosis in alveolar macrophages in vitro. METHODS: RAW264.7 cells were incubated with 200 μg/mL silica for different time periods. Cell viability was assayed by the MTT assay. Cell apoptosis was evaluated by DAPI staining, flow cytometry analysis, and Western blot analysis of caspase-3. Morphological changes in the endoplasmic reticulum were observed by transmission electron microscopy...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28804020/porcine-2-5-oligoadenylate-synthetase-2-inhibits-porcine-reproductive-and-respiratory-syndrome-virus-replication-in%C3%A2-vitro
#9
Mengmeng Zhao, Bo Wan, Huawei Li, Jian He, Xinxin Chen, Linjian Wang, Yinbiao Wang, Sha Xie, Songlin Qiao, Gaiping Zhang
Porcine reproductive and respiratory syndrome virus (PRRSV) is acknowledged a fulminating infectious pathogen affecting the pig farming industry, and current vaccines and drugs could hardly inhibit this virus. The 2', 5'-oligoadenylate synthetase (OASs) have antiviral activities, but the role(s) played by porcine OAS2 in protection against PRRSV infection are unknown. Here we found that endogenous expression of the porcine OAS2 gene could be promoted by interferon (IFN)-beta or PRRSV infection in porcine alveolar macrophages...
August 10, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28801344/frontline-science-macrophage-derived-exosomes-promote-neutrophil-necroptosis-following-hemorrhagic-shock
#10
Yang Jiao, Zhigang Li, Patricia A Loughran, Erica K Fan, Melanie J Scott, Yuehua Li, Timothy R Billiar, Mark A Wilson, Xueyin Shi, Jie Fan
Hemorrhagic shock (HS) renders patients susceptible to development of systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndrome (MODS) through mechanisms that are, as yet, unclear. Cell necroptosis, a form of regulated inflammatory cell death, is one of the mechanisms that controls cell release of inflammatory mediators from innate immune cells, such as polymorphonuclear neutrophils (PMNs), and critically regulates the progress of inflammation. In this study, we investigated the mechanisms of alveolar macrophage (AMϕ) effects on PMN necroptosis following HS...
August 11, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28798256/metabolic-characterization-and-rna-profiling-reveal-glycolytic-dependence-of-pro-fibrotic-phenotype-of-alveolar-macrophages-in-lung-fibrosis
#11
Na Xie, Huachun Cui, Jing Ge, Sami Banerjee, Sijia Guo, Shubham Dubey, Edward Abraham, Rui-Ming Liu, Gang Liu
Metabolic reprogramming has been intrinsically linked to macrophage activation. Alveolar macrophages are known to play an important role in the pathogenesis of pulmonary fibrosis. However, systematic characterization of expression profile in these cells is still lacking. Furthermore, main metabolic programs and their regulation of cellular phenotype are completely unknown. In this study, we comprehensively analyzed the expression profile and main metabolic programs in alveolar macrophages from mice with or without experimental pulmonary fibrosis...
August 10, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28798254/oxygen-dependent-changes-in-lung-development-do-not-affect-epithelial-infection-with-influenza-a-virus
#12
William Domm, Min Yee, Ravi S Misra, Robert Gelein, Aitor Nogales, Luis Martinez Sobrido, Michael A O'Reilly
Infants born prematurely often require supplemental oxygen that contributes to aberrant lung development and increased pulmonary morbidity following a respiratory viral infection. We have been using a mouse model to understand how early-life hyperoxia affects the adult lung response to influenza A virus (IAV) infection. Prior studies showed how neonatal hyperoxia (100% oxygen) increased sensitivity of adult mice to infection with influenza A virus (IAV (A/Hong Kong/X31) H3N2) as defined by persistent inflammation, pulmonary fibrosis, and mortality...
August 10, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28798047/mtor-regulates-metabolic-adaptation-of-apcs-in-the-lung-and-controls-the-outcome-of-allergic-inflammation
#13
Charles Sinclair, Gayathri Bommakanti, Luiz Gardinassi, Jens Loebbermann, Matthew Joseph Johnson, Paul Hakimpour, Thomas Hagan, Lydia Benitez, Andrei Todor, Deepa Machiah, Timothy Oriss, Anuradha Ray, Steven Bosinger, Rajesh Ravindran, Shuzhao Li, Bali Pulendran
Antigen-presenting cells (APCs) occupy diverse anatomical tissues, but their tissue-restricted homeostasis remains poorly understood. Here, working in mouse models of inflammation, we found that mTOR-dependent metabolic adaptation was required at discrete locations. mTOR was dispensable for DC homeostasis in secondary lymphoid tissues but necessary to regulate cellular metabolism and accumulation of CD103(+) DCs and alveolar macrophages in lung. Moreover, while numbers of mTOR-deficient lung CD11b(+) DCs were not changed, they were metabolically reprogrammed to skew allergic inflammation from eosinophilic Th2 to neutrophilic Th17 polarity...
August 10, 2017: Science
https://www.readbyqxmd.com/read/28798029/ppar%C3%AE-in-dendritic-cells-and-t-cells-drives-pathogenic-type-2-effector-responses-in-lung-inflammation
#14
Samuel Philip Nobs, Sara Natali, Lea Pohlmeier, Katarzyna Okreglicka, Christoph Schneider, Michael Kurrer, Federica Sallusto, Manfred Kopf
Type-2 immune responses are well-established drivers of chronic inflammatory diseases, such as asthma, and represent a large burden on public health systems. The transcription factor PPARγ is known to promote M2-macrophage and alveolar macrophage development. Here, we report that PPARγ plays a key role in both T cells and dendritic cells (DCs) for development of type-2 immune responses. It is predominantly expressed in mouse Th2 cells in vitro and in vivo as well as human Th2 cells from allergic patients...
August 10, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28778045/bleomycin-induced-pulmonary-toxicopathological-changes-in-rats-and-its-prevention-by-walnut-extract
#15
Saba Beigh, Hina Rashid, Shikha Sharma, Suhel Parvez, Sheikh Raisuddin
Oxidative stress-related inflammation and apoptosis are important pathogenic consequences, which result in acute pulmonary toxicity. Bleomycin (BLM) is used to treat various forms of cancers. However, its prolonged administration is associated with major toxicity to respiratory system. We studied the effect of walnut (Juglans regia) extract in a rat model of BLM-induced pulmonary toxicopathy. We also studied parameters of inflammation, apoptosis and oxidative stress in various groups of animals. Prophylactic treatment of total methanolic extract of walnut at the dose of 150mg/kg b...
August 1, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28774302/airway-and-parenchyma-immune-cells-in-influenza-a-h1n1-pdm09-viral-and-non-viral-diffuse-alveolar-damage
#16
Monique Buttignol, Ruy Camargo Pires-Neto, Renata Calciolari Rossi E Silva, Marina Ballarin Albino, Marisa Dolhnikoff, Thais Mauad
BACKGROUND: Diffuse alveolar damage (DAD), which is the histological surrogate for acute respiratory distress syndrome (ARDS), has a multifactorial aetiology. Therefore it is possible that the immunopathology differs among the various presentations of DAD. The aim of this study is to compare lung immunopathology of viral (influenza A(H1N1)pdm09) to non-viral, extrapulmonary aetiologies in autopsy cases with DAD. METHODS: The lung tissue of 44 patients, was divided in the H1N1 group (n = 15) characterized by severe pulmonary injury due to influenza A(H1N1)pdm09 infection; the ARDS group (n = 13), characterized by patients with DAD due to non-pulmonary causes; and the Control group (n = 16), consisting of patients with non-pulmonary causes of death...
August 3, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28769058/characterisation-of-lung-macrophage-subpopulations-in-copd-patients-and-controls
#17
Jennifer A Dewhurst, Simon Lea, Elizabeth Hardaker, Josiah V Dungwa, Arjun K Ravi, Dave Singh
Lung macrophage subpopulations have been identified based on size. We investigated characteristics of small and large macrophages in the alveolar spaces and lung interstitium of COPD patients and controls. Alveolar and interstitial cells were isolated from lung resection tissue from 88 patients. Macrophage subpopulation cell-surface expression of immunological markers and phagocytic ability were assessed by flow cytometry. Inflammatory related gene expression was measured. Alveolar and interstitial macrophages had subpopulations of small and large macrophages based on size and granularity...
August 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28765959/microrna-21-regulates-the-proliferation-and-apoptosis-of-cervical-cancer-cells-via-tumor-necrosis-factor-%C3%AE
#18
Lin Xu, Qian Xu, Xiwen Li, Xiaoling Zhang
The proliferation and apoptosis of tumor cells are regulated by a variety of microRNAs (miRs). miR‑21 can inhibit the apoptosis of cancer cells in vitro. Tumor necrosis factor α (TNF‑α) serves an important role in the induction of proliferation of cervical cancer cells. Previous studies have demonstrated that the expression level of miR‑21 is associated with TNF‑α expression in alveolar macrophages. However, to the best of our knowledge, whether miR‑21 regulates TNF‑α in cervical cells has not been reported...
August 2, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28765893/endothelial-semaphorin-7a-promotes-seawater-aspiration-induced-acute-lung-injury-through-plexin-c1-and-%C3%AE-1-integrin
#19
Minlong Zhang, Xue Yan, Wei Liu, Ruilin Sun, Yonghong Xie, Faguang Jin
Inflammation and edema are two main characteristics in seawater aspiration‑induced acute lung injury (ALI). In a previous study of the authors, it was demonstrated that endothelial semaphorin 7A (SEMA7A) serves an important role in the development of seawater‑induced inflammation and edema. However, the mechanism of endothelial SEMA7A‑mediated ALI remains unclear. Therefore, the authors explored the effect of SEMA7A in rat pulmonary microvascular endothelial cells (RPMVECs) and the interaction between endothelial SEMA7A and alveolar macrophages during seawater aspiration‑induced ALI...
July 27, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28765216/key-role-of-the-scavenger-receptor-marco-in-mediating-adenovirus-infection-and-subsequent-innate-responses-of-macrophages
#20
Mareike D Maler, Peter J Nielsen, Nicole Stichling, Idan Cohen, Zsolt Ruzsics, Connor Wood, Peggy Engelhard, Maarit Suomalainen, Ildiko Gyory, Michael Huber, Joachim Müller-Quernheim, Wolfgang W A Schamel, Siamon Gordon, Thilo Jakob, Stefan F Martin, Willi Jahnen-Dechent, Urs F Greber, Marina A Freudenberg, György Fejer
The scavenger receptor MARCO is expressed in several subsets of naive tissue-resident macrophages and has been shown to participate in the recognition of various bacterial pathogens. However, the role of MARCO in antiviral defense is largely unexplored. Here, we investigated whether MARCO might be involved in the innate sensing of infection with adenovirus and recombinant adenoviral vectors by macrophages, which elicit vigorous immune responses in vivo Using cells derived from mice, we show that adenovirus infection is significantly more efficient in MARCO-positive alveolar macrophages (AMs) and in AM-like primary macrophage lines (Max Planck Institute cells) than in MARCO-negative bone marrow-derived macrophages...
August 1, 2017: MBio
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