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scaffold ocular surface

Mehmet Erol Can, Hasan Basri Çakmak, Gamze Dereli Can, Hatice Ünverdi, Yasin Toklu, Sema Hücemenoğlu
Purpose. To investigate the effect of platelet-rich fibrin (PRF) membrane on wound healing. Methods. Twenty-four right eyes of 24 New Zealand rabbits equally divided into 2 groups for the study design. After the creation of 5 × 5 mm conjunctival damage, it was secured with PRF membrane, which was generated from the rabbit's whole blood samples in PRF membrane group, whereas damage was left unsutured in the control group. Three animals were sacrificed in each group on the 1st, 3rd, 7th, and 28th postoperative days...
2016: Journal of Ophthalmology
E Javorková, V Holáň
UNLABELLED: Regeneration of corneal epithelium is secured by a population of limbal stem cells (LSC), which are located in the basal part of the limbal epithelium. Deficiency in LSC leads to chronic inflammation, scarring and conjunctivization of cornea. Therapy of LSC deficiency consists in transplantation of limbal tissue, cultivated limbal epithelium or more recently in tranplantation of autologous cells including mesenchymal stem cells, oral mucosal epithelial cells or hair follicle-derived stem cells...
February 2016: Ceská a Slovenská Oftalmologie
Marie-Claude Robert, Mathieu Frenette, Chengxin Zhou, Yueran Yan, James Chodosh, Frederick A Jakobiec, Anna M Stagner, Demetrios Vavvas, Claes H Dohlman, Eleftherios I Paschalis
PURPOSE: To describe the fabrication, evaluation, and preliminary in vivo safety of a new drug delivery system (DDS) for topical anti-TNF-α antibody administration. METHODS: A DDS was fabricated using inverse template fabrication of a hydrophobic three-dimensional porous scaffold (100-300 μm in diameter porosity) loaded with 10% polyvinyl alcohol hydrogel carrying 5 mg/ml (weight/volume) of anti-TNF-α antibody. Drug-loaded DDS was sterilized with 25 kGy of gamma irradiation...
March 2016: Translational Vision Science & Technology
H Bai, L L Wang, Y F Huang, J X Huang
OBJECTIVE: To complete a preliminary evaluation of the feasibility of implanting the complex of mouse bone marrow mesenchymal stem cells (BMSC) and a tissue engineering scaffold into rabbit corneal lamellae, based on which a solution may be proposed to consolidate the keratoprosthesis and the recipient surface, and to reduce the risk of complications. METHODS: This experimental study was composed of two parts. (1) In vitro: some mouse BMSC were marked with red fluorescent proteins (RFP) and integrated with a decellularized pig articular cartilage extracellular matrix (ECM) scaffold...
March 2016: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
Daruosh Momenzadeh, Alireza Baradaran-Rafii, Saeed Heidari Keshel, Maryam Ebrahimi, Esmaeil Biazar
The aim of this study was to develop nanofibrous gelatin substrates for eyelid fat stem cell (EFSC) expansion that can serve as a potential alternative substrate to replace human amniotic membrane. Biocompatibility results indicated that all substrates were highly biocompatible, as EFSCs could favorably attach and proliferate on the nanofibrous surfaces. Microscopic figures showed that the EFSC were firmly anchored to the substrates and were able to retain a normal stem cell phenotype. Immunocytochemistry (ICC) and real time-PCR results revealed change in the expression profile of EFSCs grown on nanofibrous substrates when compared to those grown on control in epithelial induction condition...
February 2017: Artificial Cells, Nanomedicine, and Biotechnology
Eduardo Anitua, Francisco Muruzabal, María de la Fuente, Jesús Merayo, Juan Durán, Gorka Orive
PURPOSE: The purpose of this work is to describe and review the technology of plasma rich in growth factors (PRGF), a novel blood derivative product, in the treatment of ocular surface disorders. METHODS: To demonstrate the importance of this technology in the treatment of ocular pathologies, a thorough review of the preclinical and clinical literature results obtained following use of the different therapeutic formulations of PRGF was carried out. A literature search for applications of PGRF plasma in the ophthalmology field was carried out using the PubMed database...
July 2016: Current Eye Research
Michel Haagdorens, Sara Ilse Van Acker, Veerle Van Gerwen, Sorcha Ní Dhubhghaill, Carina Koppen, Marie-José Tassignon, Nadia Zakaria
Severe ocular surface disease can result in limbal stem cell deficiency (LSCD), a condition leading to decreased visual acuity, photophobia, and ocular pain. To restore the ocular surface in advanced stem cell deficient corneas, an autologous or allogenic limbal stem cell transplantation is performed. In recent years, the risk of secondary LSCD due to removal of large limbal grafts has been significantly reduced by the optimization of cultivated limbal epithelial transplantation (CLET). Despite the great successes of CLET, there still is room for improvement as overall success rate is 70% and visual acuity often remains suboptimal after successful transplantation...
2016: Stem Cells International
Esmaeil Biazar, Alireza Baradaran-Rafii, Saeed Heidari-keshel, Sara Tavakolifard
The aim of this study was to develop nanofibrous silk substrates for limbal stem cell expansion that can serve as a potential alternative substrate to replace human amniotic membrane. The human limbal stem cell was used to evaluate the biocompatibility of substrates (random and oriented nanofibrous mats, and human amniotic membrane) based on their phenotypic profile, viability, proliferation, and attachment ability. Biocompatibility results indicated that all substrates were highly biocompatible, as limbal stem cells could favorably attach and proliferate on the nanofibrous surfaces...
2015: Journal of Biomaterials Science. Polymer Edition
Yazad D Irani, Yuan Tian, Mengjia Wang, Sonja Klebe, Steven J McInnes, Nicolas H Voelcker, Jeffery L Coffer, Keryn A Williams
Dysfunction of corneal epithelial stem cells can result in painful and blinding disease of the ocular surface. In such cases, treatment may involve transfer of growth factor and normal adult stem cells to the ocular surface. Our purpose was to develop an implantable scaffold for the delivery of drugs and cells to the ocular surface. We examined the potential of novel composite biomaterials fabricated from electrospun polycaprolactone (PCL) fibres into which nanostructured porous silicon (pSi) microparticles of varying sizes (150-250 μm or <40 μm) had been pressed...
October 2015: Experimental Eye Research
Vladimir Holan, Peter Trosan, Cestmir Cejka, Eliska Javorkova, Alena Zajicova, Barbora Hermankova, Milada Chudickova, Jitka Cejkova
UNLABELLED: Stem cell-based therapy has become an attractive and promising approach for the treatment of severe injuries or thus-far incurable diseases. However, the use of stem cells is often limited by a shortage of available tissue-specific stem cells; therefore, other sources of stem cells are being investigated and tested. In this respect, mesenchymal stromal/stem cells (MSCs) have proven to be a promising stem cell type. In the present study, we prepared MSCs from bone marrow (BM-MSCs) or adipose tissue (Ad-MSCs) as well as limbal epithelial stem cells (LSCs), and their growth, differentiation, and secretory properties were compared...
September 2015: Stem Cells Translational Medicine
A Seyfoddin, A Chan, W-T Chen, I D Rupenthal, G I N Waterhouse, D Svirskis
Corticosteroids such as dexamethasone are first line ophthalmic treatment for non-infectious posterior uveitis. Corticosteroids are often administered via intravitreal injection to treat this condition with frequent injections associated with poor treatment adherence and complications such as endophthalmitis. Current ocular implants provide sustained corticosteroid release at predetermined rates and lack the ability for dose individualisation. This study describes the successful fabrication of electrically responsive macroporous polypyrrole (PPy) thin films, and their subsequent application to triggered dexamethasone release...
August 2015: European Journal of Pharmaceutics and Biopharmaceutics
Lihong Jiang, Pei Mou, Ruili Wei
OBJECTIVE: To explore the differential expressions of lysozyme C and lactoferrin in tears of thyroid-associated ophthalmopathy (TAO) patients versus healthy subjects by proteomics. METHODS: Tear samples were obtained from patients with active period TAO and age and gender-matched healthy subjects without symptoms of ocular surface. Then they were divided into patient and control groups. Then tear samples of two groups were analyzed. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of 15% gel was performed to determine the different protein bands in sample groups...
March 17, 2015: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Shiva Gholizadeh-Ghaleh Aziz, Sara Gholizadeh-Ghaleh Aziz, Abolfazl Akbarzadeh
Electrospinning is a technique in which materials in solution are shaped into continuous nano- and micro-sized fibers. Combining stem cells with biomaterial scaffolds and nanofibers affords a favorable approach for bone tissue engineering, stem cell growth and transfer, ocular surface reconstruction, and treatment of congenital corneal diseases. This review seeks to describe the current examples of the use of scaffolds in stem cell therapy. Stem cells are classified as adult or embryonic stem (ES) cells, and the advantages and drawbacks of each group are detailed...
August 2016: Artificial Cells, Nanomedicine, and Biotechnology
Alireza Baradaran-Rafii, Esmaeil Biazar, Saeed Heidari-keshel
PURPOSE: The aim of this study was to develop nanofibrous polycaprolactone (PCL) substrate for limbal stem cell (LSC) expansion that can serve as a potential alternative substrate to replace human amniotic membrane (AM). MATERIALS AND METHODS: The human limbus stem cell was used to evaluate the biocompatibility of substrates (nanofibrous scaffold and, human AM) based on their phenotypic profile, viability, proliferation and attachment ability. RESULTS: Biocompatibility results indicated that the all substrates were highly biocompatible, as LSCs could favorably attach and proliferate on the nanofibrous surface...
2016: Current Eye Research
Ya-Han Li, Ching-Yi Cheng, Nan-Kai Wang, Hsin-Yuan Tan, Yueh-Ju Tsai, Ching-Hsi Hsiao, David Hui-Kang Ma, Lung-Kun Yeh
OBJECTIVES: To modify a chitosan membrane (CM) by cross-linking the chitosan with genipin, a naturally occurring cross-linker extracted from Gardenia jasminoides fructus, with the aim of developing a new cell culture support and to observe the phenotypes of cultured human corneal epithelial cells (HCECs) on genipin-cross-linked chitosan membrane (GCM). METHODS: We tested the cross-linking characteristics and mechanical strength of the GCM. CMs modified by cross-linking with different concentrations of genipin were prepared to investigate the rate of membrane degradation...
February 1, 2015: Colloids and Surfaces. B, Biointerfaces
Haifeng Zhao, Mingli Qu, Yao Wang, Zhenyu Wang, Weiyun Shi
Amniotic membrane-based tissue-engineered corneal epithelium has been widely used in the reconstruction of the ocular surface. However, it often degrades too early to ensure the success of the transplanted corneal epithelium when treating patients with severe ocular surface disorders. In the present study, we investigated the preparation of xenogeneic acellular conjunctiva matrix (aCM) and evaluated its efficacy and safety as a scaffold of tissue-engineered corneal epithelium. Native porcine conjunctiva was decellularized with 0...
2014: PloS One
Xiaolin Qi, Junyi Wang, Dapeng Sun, Qingjun Zhou, Lixin Xie
PURPOSE: To investigate the morphologic changes and outcomes of the amniotic membrane as a carrier for allogeneic cultivated limbal epithelial transplantation. DESIGN: Prospective, noncomparative, interventional study. METHODS: A total of 16 eyes receiving allogeneic cultivated limbal epithelial transplantation with amniotic membrane as a carrier were enrolled. Morphologic changes in the amniotic membrane were observed by confocal microscopy and RTVue optical coherence tomography...
December 2014: American Journal of Ophthalmology
Claude Boscher, Ferenc Kuhn
PURPOSE: Anterior proliferative vitreoretinopathy (PVR) is an important cause of persistent or recurrent retinal detachment (RD). Endoscopy provides 360° panoramic viewing of the vitreous cavity and high-magnification viewing of the anterior vitreous base (AVB). This study describes the 'in vivo' anatomy and pathoanatomy of the AVB using an ocular endoscope in RD and anterior PVR. METHODS: An intraoperative analysis of over 2000 consecutive eyes undergoing vitrectomy for RD operated with endoscopy-assisted vitrectomy was performed...
June 2014: Acta Ophthalmologica
Shweta Sharma, Deepika Gupta, Sujata Mohanty, Manjeet Jassal, Ashwini K Agrawal, Radhika Tandon
PURPOSE: The purpose of this study was to modify and functionalize the surface of synthetic poly-ε-caprolactone (PCL) nanofibrous scaffolds to improve their biocompatibility in order to provide better "cell-substrate" interaction. METHODS: Poly-ε-caprolactone solution was electrospun and its surface functionality was modified by helium-oxygen (He/O2) plasma discharge. Scaffolds were characterized for their morphology, wetting ability, mechanical strength, and optical properties by using scanning electron microscopy (SEM), water contact angle measurement, tensile strength, and ultraviolet-visible (UV-Vis) spectrophotometer, respectively...
February 2014: Investigative Ophthalmology & Visual Science
Yaqing Feng, Maria Borrelli, Stephan Reichl, Stefan Schrader, Gerd Geerling
Severe ocular surface disorders can result in deficiency of limbal stem cells that is potentially associated with chronic inflammation, impaired vision and even blindness. Advanced stem cells deficiency requires reconstruction of the OS with autologous or allogeneic limbal stem cells. To address such deficiency, a limbal tissue biopsy is taken and limbal cells are expanded on a carrier, which then can be used for OS reconstruction. Human amniotic membrane - currently the most common carrier for transplantation of limbal epithelial stem cells - has the downsides of carrying the risk of disease transmission, limited transparency, variable and unstable quality and low mechanical strength...
June 2014: Current Eye Research
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