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Reactive oxygen species bacteria

I S Sazykin, M A Sazykina, L E Khmelevtsova, E Yu Seliverstova, K Sh Karchava, M V Zhuravleva
The level of catalase and superoxide dismutase induction, as well as generation of superoxide anion radical in cells and accumulation of hydrogen peroxide in the culture medium were researched in three strains of oil-degrading bacteria Achromobacter xylosoxidans at cultivation in rich nutrient medium and in the media with hydrocarbons as the only source of carbon. The effects of pentane, decane, hexadecane, cyclohexane, benzene, naphthalene and diesel fuel were evaluated. It was determined that in the microbial cell on media with hydrocarbons, the generation of superoxide anion radical increases, accumulation of hydrogen peroxide and induction of superoxide dismutase synthesis occur, and catalase activity is reduced...
April 23, 2018: Archives of Microbiology
Sławomir Jaworski, Mateusz Wierzbicki, Ewa Sawosz, Anna Jung, Grzegorz Gielerak, Joanna Biernat, Henryk Jaremek, Witold Łojkowski, Bartosz Woźniak, Jacek Wojnarowicz, Leszek Stobiński, Artur Małolepszy, Marta Mazurkiewicz-Pawlicka, Maciej Łojkowski, Natalia Kurantowicz, André Chwalibog
One of the most promising methods against drug-resistant bacteria can be surface-modified materials with biocidal nanoparticles and nanocomposites. Herein, we present a nanocomposite with silver nanoparticles (Ag-NPs) on the surface of graphene oxide (GO) as a novel multifunctional antibacterial and antifungal material. Ultrasonic technologies have been used as an effective method of coating polyurethane foils. Toxicity on gram-negative bacteria (Escherichia coli), gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis), and pathogenic yeast (Candida albicans) was evaluated by analysis of cell morphology, assessment of cell viability using the PrestoBlue assay, analysis of cell membrane integrity using the lactate dehydrogenase assay, and reactive oxygen species production...
April 23, 2018: Nanoscale Research Letters
Filimon Mitiku, Carol A Hartley, Fiona M Sansom, Joanne E Coombe, Peter D Mansell, David S Beggs, Glenn F Browning
Mycoplasma bovis has been increasingly recognised worldwide as an economically important pathogen of cattle, causing a range of diseases, including pneumonia, mastitis, polyarthritis and otitis media. It is believed that M. bovis utilises a range of cell surface proteins, including nucleases, to evade the host immune response and survive. However, despite the importance of neutrophils in controlling pathogenic bacteria, the interaction between these cells and M. bovis is not well-characterised. In addition to phagocytosis, neutrophils combat pathogens through the release of neutrophil extracellular traps (NETs), which are composed of their nuclear and granular components, including DNA...
May 2018: Veterinary Microbiology
Vanesa Pérez-Laguna, Ana J García-Malinis, Carmen Aspiroz, Antonio Rezusta, Yolanda Gilaberte
The microorganisms that cause infections are increasing their resistance to antibiotics. In this context, alternative treatments are necessary. The antimicrobial photodynamic therapy (aPDT) is a therapeutic modality based on photosensitizing molecules that end up generating reactive oxygen species that induce the destruction of the target cells when are irradiated with light of a suitable wavelength and at a proper dose. The cells targeted by aPDT are all types of microorganisms (bacteria, fungi and parasites) including viruses and has been proven effective against representative members of all of them...
April 19, 2018: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
Anutthaman Parthasarathy, Penelope J Cross, Renwick C J Dobson, Lily E Adams, Michael A Savka, André O Hudson
Tyrosine, phenylalanine and tryptophan are the three aromatic amino acids (AAA) involved in protein synthesis. These amino acids and their metabolism are linked to the synthesis of a variety of secondary metabolites, a subset of which are involved in numerous anabolic pathways responsible for the synthesis of pigment compounds, plant hormones and biological polymers, to name a few. In addition, these metabolites derived from the AAA pathways mediate the transmission of nervous signals, quench reactive oxygen species in the brain, and are involved in the vast palette of animal coloration among others pathways...
2018: Frontiers in Molecular Biosciences
Heloisa N Pantaroto, Antonio P Ricomini-Filho, Martinna M Bertolini, José H D Silva, Nilton F Azevedo Neto, Cortino Sukotjo, Elidiane C Rangel, Valentim A R Barão
OBJECTIVE: Titanium dioxide (TiO2 ) incorporation in biomaterials is a promising technology due to its photocatalytic and antibacterial activities. However, the antibacterial potential of different TiO2 crystalline structures on a multispecies oral biofilm remains unknown. We hypothesized that the different crystalline TiO2 phases present different photocatalytic and antibacterial activities. METHODS: Three crystalline TiO2 films were deposited by magnetron sputtering on commercially pure titanium (cpTi), in order to obtain four groups: (1) machined cpTi (control); (2) A-TiO2 (anatase); (3) M-TiO2 (mixture of anatase and rutile); (4) R-TiO2 (rutile)...
April 17, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
Ting Feng, Jialiang Liang, Zhiyao Ma, Mian Li, Meiping Tong
BiOBr-AgBr hybrid photocatalysts were synthesized via a facile precipitation and in-situ ion exchange method at room temperature. The as-synthesized photocatalysts were well characterized with different techniques. The disinfection activities of BiOBr-AgBr towards model bacterial strain Gram-negative Escherichia coli (E. coli) were tested under both dark and visible light irradiation conditions. 1 × 107 CFU/mL of E. coli cells could be completely inactivated by BiOBr-AgBr with the optimal silver amount (BiOBr-0...
April 8, 2018: Colloids and Surfaces. B, Biointerfaces
Uliana Ya Stambulska, Maria M Bayliak, Volodymyr I Lushchak
Most legume species have the ability to establish a symbiotic relationship with soil nitrogen-fixing rhizobacteria that promote plant growth and productivity. There is an increasing evidence of reactive oxygen species (ROS) important role in formation of legume-rhizobium symbiosis and nodule functioning. Environmental pollutants such as chromium compounds can cause damage to rhizobia, legumes, and their symbiosis. In plants, toxic effects of chromium(VI) compounds are associated with the increased production of ROS and oxidative stress development as well as with inhibition of pigment synthesis and modification of virtually all cellular components...
2018: BioMed Research International
Domenico Ricucci, Simona Loghin, Li-Na Niu, Franklin R Tay
INTRODUCTION: The present study reported the histological events that occurred in the radicular pulp of human mature teeth in the presence of medium/deep untreated caries lesions, and those teeth with restorations or direct pulp capping, with particular emphasis on the morphology of the canal wall dentine and the odontoblast layer. METHODS: Sixty-two teeth with medium/deep caries lesions, extensive restorations or after application of a direct pulp capping procedure were obtained from 57 subjects...
April 13, 2018: Journal of Dentistry
Sangiliyandi Gurunathan, Yun-Jung Choi, Jin-Hoi Kim
Bovine postpartum diseases remain one of the most significant and highly prevalent illnesses with negative effects on the productivity, survival, and welfare of dairy cows. Antibiotics are generally considered beneficial in the treatment of endometritis; however, frequent usage of each antibiotic drug is reason for the emergence of multidrug resistance (MDR) of the pathogenic microorganisms, representing a major impediment for the successful diagnosis and management of infectious diseases in both humans and animals...
April 16, 2018: International Journal of Molecular Sciences
Nadia Riera, Han Wang, Yong Li, Jinyun Li, Kirsten Pelz-Stelinski, Nian Wang
Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is an important citrus disease that causes significant economic losses worldwide. All commercial citrus varieties are susceptible to citrus canker. Currently, chemical control with copper based products is the main approach to control Xcc dispersal and plant colonization. However, extensive use of copper compounds can result in copper-resistant strains and cause adverse effects on the environment. Alternatives to chemical control involve the activation of citrus immunity to control the disease...
April 12, 2018: Phytopathology
Zhenyu Jin, Jiahong Li, Lei Ni, Rongrong Zhang, Aiguo Xia, Fan Jin
Understanding the mechanisms that promote cooperative behaviors of bacteria in their hosts is of great significance to clinical therapies. Environmental stress is generally believed to increase competition and reduce cooperation in bacteria. Here, we show that bacterial cooperation can in fact be maintained because of environmental stress. We show that Pseudomonas aeruginosa regulates the secretion of iron-scavenging siderophores in the presence of different environmental stresses, reserving this public good for private use in protection against reactive oxygen species when under stress...
April 11, 2018: Nature Communications
Tian Luo, Shubhajit Mitra, Jere W McBride
Ehrlichia chaffeensis is an obligately intracellular bacterium that exhibits tropism for mononuclear phagocytes. The mechanisms involved in E. chaffeensis infection of the host cell and evasion of host defenses are not fully defined, but a subset of type 1 secreted tandem repeat protein (TRP) effectors play important roles. Recently, we determined molecular interactions of TRP120, TRP47, and TRP32 with the eukaryotic host cell. In this investigation, we used yeast two-hybrid analysis to reveal that another E...
April 25, 2018: MSphere
Li Guo, Ruobing Xu, Yiming Zhao, Dingxin Liu, Zhijie Liu, Xiaohua Wang, Hailan Chen, Michael G Kong
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of serious nosocomial infections, and recurrent MRSA infections primarily result from the survival of persister cells after antibiotic treatment. Gas plasma, a novel source of ROS (reactive oxygen species) and RNS (reactive nitrogen species) generation, not only inactivates pathogenic microbes but also restore the sensitivity of MRSA to antibiotics. This study further found that sublethal treatment of MRSA with both plasma and plasma-activated saline increased the antibiotic sensitivity and promoted the eradication of persister cells by tetracycline, gentamycin, clindamycin, chloramphenicol, ciprofloxacin, rifampicin, and vancomycin...
2018: Frontiers in Microbiology
B Suyamud, P Thiravetyan, B Panyapinyopol, D Inthorn
Bisphenol A (BPA) is one of the most abundant endocrine-disrupting compounds which is found in the aquatic environment. However, actual knowledge regarding the effect of plant-bacteria interactions on enhancing BPA removal is still lacking. In the present study, Dracaena sanderiana endophytic bacteria interactions were investigated to evaluate the effect of bacterial inoculation on BPA removal under hydroponic conditions. Two plant growth-promoting (PGP) bacterial strains, Bacillus thuringiensis and Pantoea dispersa, which have high BPA tolerance and can utilize BPA for growth, were used as plant inocula...
April 5, 2018: Ecotoxicology and Environmental Safety
Syeda M Hussain, Valdo Rodrigues Herling, Paulo Henrique Mazza Rodrigues, Ishrat Naz, Hamayun Khan, Muhammad Tahir Khan
Photosensitization is severe dermatitis or oxidative/chemical changes in the epidermal tissues activated by the light-induced excitation of molecules within the tissue. It is a series of reactions mediated through light receptors and is more common when the plant-produced metabolites are heterocyclic/polyphenols in nature. The areas affected are exposed body parts and mostly non-pigmented areas with least ultraviolet protection. Similarly, cellular alteration also occurs in the affected animal's dermal tissues and body parts and grazing animals by the accumulation and activation of photodynamic molecules...
April 6, 2018: Tropical Animal Health and Production
Hee Geun Park, Kwang Sik Lee, Bo Yeon Kim, Hyung Joo Yoon, Yong Soo Choi, Kyung Yong Lee, Hu Wan, Jianhong Li, Byung Rae Jin
Honeybee (Apis mellifera) egg-yolk protein vitellogenin (Vg) plays roles in immunity, antioxidation, and life span beyond reproduction, but it also acts as an allergen Api m 12 in venom. Here we established antimicrobial and antioxidant roles of honeybee Vg in the body and venom. Using the cDNA encoding Vg identified from Asiatic honeybee (A. cerana) workers, recombinant A. cerana Vg (AcVg) protein of approximately 180 kDa was produced in baculovirus-infected insect cells. In A. cerana worker bees, AcVg was expressed in the fat body and venom gland and was present in the secreted venom...
April 2, 2018: Developmental and Comparative Immunology
Jiancheng Dong, Reza A Ghiladi, Qingqing Wang, Yibing Cai, Qufu Wei
Towards the development of anti-infective nanoscale materials employing a photodynamic mechanism of action, we report the synthesis, physical properties (SEM, mechanical strength, water contact angle), spectroscopic characterization (infrared, Raman, DRUV), and evaluation of antibacterial efficacy of porphyrin-conjugated regenerated cellulose nanofibers, termed RC-TETA-PPIX-Zn. Cellulose acetate was electrospun to produce nanofibers, thermally treated to enhance mechanical strength, and finally hydrolyzed to produce regenerated cellulose (RC) nanofibers that possessed a high surface area and nanofibrous structure...
April 3, 2018: Nanotechnology
Natalia V Motorna, Svetlana L Rybalko, Daria B Starosyla, Mykhailo M Guzyk, Iryna G Strokina, Rostyslav F Kaminsky, Alina V Korsak, Sergey I Savosko, Liudmyla M Sokurenko, Yurii B Chaikovsky
OBJECTIVE: Inrtoduction: Post-stroke complications are one of the urgent and insufficiently resolved problems. According to different literature data 23% to 65% of patients suffer from the post-stroke development of an infectious process. Herpes simplex virus type 1 and 2 can also be etiological factors of stroke development, however their reactivation is seldom mentioned in clinical observations. The development of immune suppression is considered to be the cause of these complications...
2018: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
Vladimir N Popok, Cesarino M Jeppesen, Peter Fojan, Anna Kuzminova, Jan Hanuš, Ondřej Kylián
Background: Antibacterial materials are of high importance for medicine, and for the production and conservation of food. Among these materials, polymer films with metal nanoparticles (NPs) are of considerable interest for many practical applications. Results: The paper describes a novel approach for the formation of bactericidal polymer thin films (polystyrene in this case), produced by spin-coating, with Ti and Cu NPs deposited from cluster beams. Ti NPs are treated in three different ways in order to study different approaches for oxidation and, thus, efficiency in formation of the particles with semiconducting properties required for the catalytic formation of reactive oxygen species...
2018: Beilstein Journal of Nanotechnology
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