keyword
MENU ▼
Read by QxMD icon Read
search

Peptide, protein

keyword
https://www.readbyqxmd.com/read/29684875/toxicoproteomic-analysis-of-human-lung-epithelial-cells-exposed-to-steel-industry-ambient-particulate-matter-pm-reveals-possible-mechanism-of-pm-related-carcinogenesis
#1
S Senthil Kumar, P Muthuselvam, V Pugalenthi, N Subramanian, K M Ramkumar, T Suresh, T Suzuki, P Rajaguru
Toxicoproteomic analysis of steel industry ambient particulate matter (PM) that contain high concentrations of PAHs and metals was done by treating human lung cancer cell-line, A549 and the cell lysates were analysed using quantitative label-free nano LC-MS/MS. A total of 18,562 peptides representing 1576 proteins were identified and quantified, with 196 proteins had significantly altered expression in the treated cells. Enrichment analyses revealed that proteins associated to redox homeostsis, metabolism, and cellular energy generation were inhibited while, proteins related to DNA damage and repair and other stresses were over expressed...
April 20, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29684684/targeted-data-independent-acquisition-for-mass-spectrometric-detection-of-ras-mutations-in-formalin-fixed-paraffin-embedded-tumor-biopsies
#2
Yeoun Jin Kim, Andrew G Chambers, Fabiola Cecchi, Todd Hembrough
Genomic testing for KRAS and NRAS mutations in clinical biopsies of various cancers is routinely performed to predict futility of anti-epidermal growth factor receptor (anti-EGFR) therapies. We hypothesized that RAS mutations could be detected and quantified at the protein level for diagnostic purposes using data-independent acquisition (DIA)-based mass spectrometry in formalin-fixed, paraffin-embedded (FFPE) tumor samples. We developed a targeted DIA assay that surveys the specific mass range of all possible peptides harboring activating mutations in KRAS exon 2...
April 20, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29684595/apelin-and-elabela-toddler-double-ligands-for-apj-apelin-receptor-in-heart-development-physiology-and-pathology
#3
Keiji Kuba, Teruki Sato, Yumiko Imai, Tomokazu Yamaguchi
Apelin is an endogenous peptide ligand for the G protein-coupled receptor APJ/AGTRL1/APLNR and is widely expressed throughout human body. In adult hearts Apelin-APJ/Apelin receptor axis is potently inotropic, vasodilatory, and pro-angiogenic and thereby contributes to maintaining homeostasis in normal and pathological hearts. Apelin-APJ/Apelin receptor is also involved in heart development including endoderm differentiation, heart morphogenesis, and coronary vascular formation. APJ/Apelin receptor had been originally identified as an orphan receptor for its sequence similarity to Angiotensin II type 1 receptor, and it was later deorphanized by identification of Apelin in 1998...
April 20, 2018: Peptides
https://www.readbyqxmd.com/read/29684589/hydrogen-sulfide-donor-nahs-stimulates-anp-secretion-via-the-k-atp-channel-and-the-nos-sgc-pathway-in-rat-atria
#4
Lamei Yu, Byung Mun Park, Yong Jin Ahn, Gi-Ja Lee, Suhn Hee Kim
Hydrogen sulfide (H2 S) is normally produced from L-cysteine in mammalian tissues and related to the pathogenesis of cardiovascular diseases. The aim of this study is to investigate the effects of H2 S donor on atrial natriuretic peptide (ANP) secretion and define its mechanism using normal and isoproterenol (ISP)-treated rats. Several H2 S donors were perfused into isolated beating rat atria, and atrial pressure (AP) and ANP secretion were measured. NaHS augmented high stretch-induced ANP secretion and decreased AP in a dose-dependent manner...
April 20, 2018: Peptides
https://www.readbyqxmd.com/read/29684535/sodium-caprate-enables-the-blood-pressure-lowering-effect-of-ile-pro-pro-and-leu-lys-pro-in-spontaneously-hypertensive-rats-by-indirectly-overcoming-pept1-inhibition
#5
John P Gleeson, Jesús M Frías, Sinéad M Ryan, David J Brayden
The tripeptides, Ile-Pro-Pro (IPP) and Leu-Lys-Pro (LKP), inhibit angiotensin-converting enzyme (ACE) resulting in lowered blood pressure. Our hypothesis was that the medium chain fatty acid permeation enhancer, sodium caprate (C10 ), may prevent the decrease in permeability of the tripeptides when PepT1 is inhibited by glycyl-sarcosine (Gly-Sar), a situation that may occur in the presence of food hydrolysates. Using Caco-2 monolayers and isolated rat jejunal tissue, the apparent permeability coefficients (Papp ) of [3 H]-IPP and [3 H]-LKP were assessed in the presence of Gly-Sar with and without C10 ...
April 20, 2018: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29684497/targeted-thermal-therapy-with-genetically-engineered-magnetite-magnetosomes-rgd-photothermia-is-far-more-efficient-than-magnetic-hyperthermia
#6
Anouchka Plan, Sandra Preveral, Alberto Curcio, Amanda Silva, Chistopher T Lefèvre, David Pignol, Yoann Lalatonne, Claire Wilhelm
Providing appropriate means for heat generation by low intratumoral nanoparticle concentrations is a major challenge for cancer nanotherapy. Here we propose RGD-tagged magnetosomes (magnetosomes@RGD) as a biogenic, genetically engineered, inorganic platform for multivalent thermal cancer treatment. Magnetosomes@RGD are biomagnetite nanoparticles synthesized by genetically modified magnetotactic bacteria thanks to a translational fusion of the RGD peptide with the magnetosomal protein MamC. Magnetosomes@RGD thus combine the high crystallinity of their magnetite core with efficient surface functionalization...
April 20, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29684394/n-acetyl-seryl-aspartyl-lysyl-proline-ac-sdkp-attenuates-silicotic-fibrosis-by-suppressing-apoptosis-of-alveolar-type-ii-epithelial-cells-via-mediation-of-endoplasmic-reticulum-stress
#7
Lijuan Zhang, Dingjie Xu, Qian Li, Yi Yang, Hong Xu, Zhongqiu Wei, Ruimin Wang, Wenli Zhang, Yan Liu, Yucong Geng, Shifeng Li, Xuemin Gao, Fang Yang
Damage to alveolar epithelial cells (AECs) caused by long-term inhalation of large amounts of silica dust plays a significant role in the pathology of silicosis. The present study was undertaken to investigate the regulatory mechanism(s) involved in type II AEC damage from silicon dioxide (SiO2 ) as well as the mechanism(s) related to the prevention of silicosis by the antifibrotic tetra peptide, N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP). The 2-DE results showed that SiO2 induced endoplasmic reticulum (ER) stress in A549 cells...
April 20, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29684334/the-mkk7-inhibitor-peptide-gadd45%C3%AE-i-attenuates-er-stress-induced-mitochondrial-dysfunction-in-ht22-cells-involvement-of-jnk-wnt-pathway
#8
Guang-Hua Xu, Bing-Jun Song, Dan Liu, Yu-Hua Chen, Yuan Zhou, Wen-Bo Liu, Hua Li, Tian-Lin Long, Rui Zhang, Wei Liu
JNK, a member of the mitogen activated protein kinases (MAPKs) superfamily, plays a key role in cell death in many neurological disorders, but systemic inhibition of JNK has detrimental side effects. JNK can be regulated by two direct upstream kinases: MAPK kinase 4 (MKK4) and MAPK kinase 7 (MKK7). Here, we investigated the effect of GADD45β-I, a recently designed cell-permeable inhibitor peptide for MKK7, on endoplasmic reticulum (ER) stress-induced cytotoxicity in neuronal HT22 cells. We found that treatment with the ER stress inducer tunicamycin (TM) increased the phosphorylation of JNK and MKK7 in HT22 cells, which was nullified by GADD45β-I...
April 20, 2018: Brain Research
https://www.readbyqxmd.com/read/29684330/novel-bioactive-peptides-from-pd-l1-2-a-type-1-ribosome-inactivating-protein-from-phytolacca-dioica-l-evaluation-of-their-antimicrobial-properties-and-anti-biofilm-activities
#9
Elio Pizzo, Katia Pane, Andrea Bosso, Nicola Landi, Sara Ragucci, Rosita Russo, Rosa Gaglione, Marcelo D T Torres, Cesar de la Fuente-Nunez, Angela Arciello, Alberto Di Donato, Eugenio Notomista, Antimo Di Maro
Antimicrobial peptides, also called Host Defence Peptides (HDPs), are effectors of innate immune response found in all living organisms. In a previous report, we have identified by chemical fragmentation, and characterized the first cryptic antimicrobial peptide in PD-L4, a type 1 ribosome-inactivating protein (RIP) from leaves of Phytolacca dioica L. We applied a recently developed bioinformatic approach to a further member of the differently expressed pool of type 1 RIPs from P. dioica (PD-L1/2), and identified two novel putative cryptic HDPs in its N-terminal domain...
April 20, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29684281/superactivity-of-mof-808-towards-peptide-bond-hydrolysis
#10
Hong Giang T Ly, Guangxia Fu, Aleksandar Kondinski, Bart Bueken, Dirk De Vos, Tatjana N Parac-Vogt
MOF-808, a Zr(IV)-based metal-organic framework, has been proven to be a very effective heterogeneous catalyst for the hydrolysis of the peptide bond in a wide range of peptides and in hen egg white lysozyme protein. The kinetic experiments with a series of Gly-X dipeptides with varying nature of amino acid side chain have shown that MOF-808 exhibits selectivity depending on the size and chemical nature of the X side chain. Dipeptides with smaller or hydrophilic residues were hydrolyzed faster than those with bulky and hydrophobic residues that lack electron rich functionalities which could engage in favourable intermolecular interactions with the btc linkers...
April 23, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29683766/displaying-31rg-1-peptide-on-the-surface-of-hpv16-l1-by-use-of-a-human-papillomavirus-chimeric-virus-like-particle-induces-cross-neutralizing-antibody-responses-in-mice
#11
Xue Chen, Ting Zhang, Hongyang Liu, Yaru Hao, Guoyang Liao, Xuemei Xu
Current available human papillomavirus (HPV) vaccines are based on the major capsid protein L1 virus-like particles (VLPs), which mainly induce type-specific neutralizing antibodies against vaccine types. Continuing to add more types of VLPs in a vaccine raises the complexity and cost of production which remains the principal impediment to achieve broad implementation of HPV vaccines, particularly in developing regions. In this study, we constructed 16L1-31L2 chimeric VLP (cVLP) by displaying HPV31 L2 aa.17-38 on the h4 coil surface region of HPV16 L1, and assessed its immunogenicity in mouse model...
April 23, 2018: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29683649/amyloid-%C3%AE-peptide-interactions-with-amphiphilic-surfactants-electrostatic-and-hydrophobic-effects
#12
Nicklas Österlund, Yashraj S Kulkarni, Agata D Misiaszek, Cecilia Wallin, Dennis M Krüger, Qinghua Liao, Farshid Mashayekhy Rad, Jüri Jarvet, Birgit Strodel, Sebastian K T S Wärmländer, Leopold L Ilag, Shina C L Kamerlin, Astrid Gräslund
The amphiphilic nature of the amyloid-β (Aβ) peptide associated with Alzheimer's disease facilitates various interactions with biomolecules such as lipids and proteins, with effects on both structure and toxicity of the peptide. Here, we investigate these peptide-amphiphile interactions by experimental and computational studies of Aβ(1-40) in the presence of surfactants with varying physico-chemical properties. Our findings indicate that electrostatic peptide-surfactant interactions are required for co-clustering and structure induction in the peptide and that the strength of the interaction depends on the surfactant net charge...
April 23, 2018: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29683528/microchip-electrophoresis-separation-of-a-panel-of-preterm-birth-biomarkers
#13
Anna V Nielsen, Jacob B Nielsen, Radim Knob, Vishal Sahore, Adam T Woolley
Preterm birth (PTB) is responsible for over one million infant deaths annually worldwide. Often, the first and only indication of PTB risk is the onset of early labor. Thus, there is an urgent need for an early PTB risk diagnostic that is inexpensive, reliable, and robust. Here, we describe the development of a microchip electrophoresis (μCE) method for separating a mixture of six PTB protein and peptide biomarkers present in maternal blood serum. μCE devices were photografted with a poly(ethylene glycol) diacrylate surface coating to regulate EOF and reduce non-specific analyte adsorption...
April 23, 2018: Electrophoresis
https://www.readbyqxmd.com/read/29683502/the-c-terminus-of-the-amelogenin-peptide-influences-the-proliferation-of-human-bone-marrow-mesenchymal-stem-cells
#14
Ryo Kunimatsu, Tetsuya Awada, Yuki Yoshimi, Kazuyo Ando, Naoto Hirose, Yuki Tanne, Keisuke Sumi, Kotaro Tanimoto
BACKGROUND: Amelogenins are a family of enamel matrix proteins that are important for formation of enamel. Amelogenins may induce division of mesenchymal stem cells (MSCs), among others. Recently, the C-terminus of the amelogenin peptide (AMG-CP) has been shown to enhance the proliferation of cementoblast lineage cells. The role of the amelogenin peptide on the proliferation of human MSCs and related alterations in the intracellular signaling pathway were studied. METHODS: MSCs were exposed to AMG-CP in vitro...
April 2018: Journal of Periodontology
https://www.readbyqxmd.com/read/29683469/solid-state-electrical-applications-of-protein-and-peptide-based-nanomaterials
#15
REVIEW
Sayak Subhra Panda, Howard E Katz, John D Tovar
The field of organic electronics continues to be driven by new charge-transporting materials that are typically processed from toxic organic solvents incompatible with biological environments. Over the past few decades, powerful examples of electrical transport as mediated through protein-based macromolecules have fueled the emerging area of organic bioelectronics. These attractive bioinspired architectures have enabled several important applications that draw on their functional electrical properties, ranging from field-effect transistors to piezoelectrics...
April 23, 2018: Chemical Society Reviews
https://www.readbyqxmd.com/read/29683460/quantification-of-site-specific-protein-lysine-acetylation-and-succinylation-stoichiometry-using-data-independent-acquisition-mass-spectrometry
#16
Lei Wei, Jesse G Meyer, Birgit Schilling
Post-translational modification (PTM) of protein lysine residues by NƐ-acylation induces structural changes that can dynamically regulate protein functions, for example, by changing enzymatic activity or by mediating interactions. Precise quantification of site-specific protein acylation occupancy, or stoichiometry, is essential for understanding the functional consequences of both global low-level stoichiometry and individual high-level acylation stoichiometry of specific lysine residues. Other groups have reported measurement of lysine acetylation stoichiometry by comparing the ratio of peptide precursor isotopes from endogenous, natural abundance acylation and exogenous, heavy isotope-labeled acylation introduced after quantitative chemical acetylation of proteins using stable isotope-labeled acetic anhydride...
April 4, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29683360/hydrogen-rich-water-improves-cognitive-impairment-gender-dependently-in-app-ps1-mice-without-affecting-a%C3%AE-clearance
#17
Chen Hou, Yunhua Peng, Chuan Qin, Fan Fan, Jiankang Liu, Jiangang Long
Alzheimer's disease (AD) is characterised as a provoked inflammatory response and oxidative stress along with amyloid β peptide (Aβ) deposition and neurofibrillary tangles in the brain, and effective treatment is greatly needed. Molecular hydrogen, which has been proposed to be an antioxidant that selectively reduces reactive oxygen species, was found to exert beneficial effects in Aβ injection-induced cognitive dysfunction. However, whether and how hydrogen affects AD pathogenesis remains uninvestigated...
April 23, 2018: Free Radical Research
https://www.readbyqxmd.com/read/29683093/a-novel-fluorescence-based-screen-for-inhibitors-of-the-initiation-of-dna-replication-in-bacteria
#18
Rasmus N Klitgaard, Anders Lobner-Olesen
BACKGROUND: One of many strategies to overcome antibiotic resistance is the discovery of compounds targeting cellular processes, which have not yet been exploited. METHODS AND MATERIALS: Using various genetic tools, we constructed a novel high throughput, cell based, fluorescence screen for inhibitors of chromosome replication initiation in bacteria. RESULTS: The screen was validated by expression of an intra-cellular cyclic peptide interfering with the initiator protein DnaA and by over-expression of the negative initiation regulator SeqA...
April 22, 2018: Current Drug Discovery Technologies
https://www.readbyqxmd.com/read/29682972/parsing-and-quantification-of-raw-orbitrap-mass-spectrometer-data-using-rawquant
#19
Kevin A Kovalchik, Sophie Moggridge, David D Y Chen, Gregg B Morin, Christopher S Hughes
Effective analysis of protein samples by mass spectrometry (MS) requires careful selection and optimization of a range of experimental parameters. As the output from the primary detection device, the 'raw' MS data file can be used to gauge the success of a given sample analysis. However, the closed-source nature of the standard raw MS file can complicate effective parsing of the data contained within. To ease and increase the range of analyses possible, the RawQuant tool was developed to enable parsing of raw MS files derived from Thermo Orbitrap instruments to yield meta and scan data in an openly readable text format...
April 23, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29682869/beryllium-complexes-with-bio-relevant-functional-groups-coordination-geometries-and-binding-affinities
#20
Matthias Müller, Magnus Richard Buchner
The coordination mode around beryllium in proteins and the binding affinity towards the peptide are unknown, since there have been no coordination compounds of beryllium with ligands bearing bio-relevant functional groups. We report the first comprehensive study on Be-complexes with monodentate carboxylic acids, esters, aldehydes and alcohols. Through solution and solid state techniques we determined that the binding affinities of Be2+ towards the functional groups are: carboxylate > alcohol > aldehyde > ester...
April 22, 2018: Angewandte Chemie
keyword
keyword
67427
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"