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https://www.readbyqxmd.com/read/28340485/an-approach-for-liposome-immobilization-using-sterically-stabilized-micelles-ssms-as-a-precursor-for-bio-layer-interferometry-based-interaction-studies
#1
Jakob Wallner, Gabriele Lhota, Markus Schosserer, Karola Vorauer-Uhl
Non-fluidic bio-layer interferometry (BLI) has rapidly become a standard tool for monitoring almost all biomolecular interactions in a label-free, real-time and high-throughput manner. High-efficiency screening methods which measure the kinetics of liposomes with a variety of compounds require the immobilization of liposomes. In this work, a method is described for immobilizing liposomes for interaction studies, based on the biophysical principles of this biosensor platform. The immobilization approach includes the loading of DSPE-PEG(2000)-biotin containing sterically stabilized micelles (SSMs) which are restructured in a buffer change step, resulting in an accessible substrate for liposome immobilization...
March 12, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28340481/metabolic-profiling-of-tyrosine-tryptophan-and-glutamate-in-human-urine-using-gas-chromatography-tandem-mass-spectrometry-combined-with-single-spe-cleanup
#2
Hyun Ju Shin, Na Hyun Park, Wonwoong Lee, Man Ho Choi, Bong Chul Chung, Jongki Hong
The tyrosine, tryptophan, and glutamate metabolic pathways play key roles on pathological state of neuronal functions and the change of their levels in biological systems reflects the progress degree of neuronal diseases. Comprehensive profiling of these metabolites is important to find new biomarkers for diagnosis or prognosis of various neuronal diseases. However, the overall profiling analysis of various neurochemicals in biological sample is confronted with several limitations due to their low concentration and physicochemical properties and the coexistence of matrices...
March 16, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28340480/using-uhplc-q-trap-ms-as-a-complementary-technique-to-in-depth-mine-uplc-q-tof-ms-data-for-identifying-modified-nucleosides-in-urine
#3
Zhiwei Lu, Qing Wang, Meiling Wang, Shuang Fu, Qingqing Zhang, Zhixin Zhang, Huizhen Zhao, Yuehong Liu, Zhenhai Huang, Ziye Xie, Honghong Yu, Xiaoyan Gao
Modified nucleosides, metabolites of RNA, are potential biomarkers of cancer before the appearance of morphological abnormalities. It is of great significance to comprehensively detect and identify nucleosides in human urine for discovery of cancer biomarkers. However, the lower abundance, the greater polarity and the matrix effects make it difficult to detect urinary nucleosides. In this paper, an integrated method consisted of sample preparation followed by ultraperformance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC Q-TOF/MS) detection and primary identification, then ultra-high performance liquid chromatography coupled with hybrid triple quadrupole linear ion trap mass spectrometer (UHPLC Q-Trap/MS) further identification and validation were introduced...
March 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28340479/additive-effects-prevail-the-response-of-biota-to-multiple-stressors-in-an-intensively-monitored-watershed
#4
Alexander Gieswein, Daniel Hering, Christian K Feld
Freshwater ecosystems are impacted by a range of stressors arising from diverse human-caused land and water uses. Identifying the relative importance of single stressors and understanding how multiple stressors interact and jointly affect biology is crucial for River Basin Management. This study addressed multiple human-induced stressors and their effects on the aquatic flora and fauna based on data from standard WFD monitoring schemes. For altogether 1095 sites within a mountainous catchment, we used 12 stressor variables covering three different stressor groups: riparian land use, physical habitat quality and nutrient enrichment...
March 21, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28340474/studying-the-effects-of-natural-extracts-with-metabolomics-a-longitudinal-study-on-the-supplementation-of-healthy-rats-with-polygonum-cuspidatum-sieb-et-zucc
#5
Gregorio Peron, Jalal Uddin, Matteo Stocchero, Stefano Mammi, Elisabetta Schievano, Stefano Dall'Acqua
BACKGROUND: A longitudinal study was performed to evaluate the effects of Polygonum cuspidatum extract (standardized at 20% resveratrol) supplementation on healthy rats. The effects were explored by monitoring urinary metabolome changes using UPLC-HRMS and (1)H NMR-based approaches. The aim of the study was to explore the effects of P. cuspidatum supplementation on a healthy animal model using metabolomics, in order to determine possible modes of action and obtain information on bioactivity...
March 16, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28340465/dumbbell-dna-templated-cunps-as-a-nano-fluorescent-probe-for-detection-of-enzymes-involved-in-ligase-mediated-dna-repair
#6
Taiping Qing, Xiaoxiao He, Dinggeng He, Xiaosheng Ye, Jingfang Shangguan, Jinquan Liu, Baoyin Yuan, Kemin Wang
DNA repair processes are responsible for maintaining genome stability. Ligase and polynucleotide kinase (PNK) have important roles in ligase-mediated DNA repair. The development of analytical methods to monitor these enzymes involved in DNA repair pathways is of great interest in biochemistry and biotechnology. In this work, we reported a new strategy for label-free monitoring PNK and ligase activity by using dumbbell-shaped DNA templated copper nanoparticles (CuNPs). In the presence of PNK and ligase, the dumbbell-shaped DNA probe (DP) was locked and could resist the digestion of exonucleases and then served as an efficient template for synthesizing fluorescent CuNPs...
March 21, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28340464/a-review-on-various-electrochemical-techniques-for-heavy-metal-ions-detection-with-different-sensing-platforms
#7
REVIEW
BabanKumar Bansod, Tejinder Kumar, Ritula Thakur, Shakshi Rana, Inderbir Singh
Heavy metal ions are non-biodegradable and contaminate most of the natural resources occurring in the environment including water. Some of the heavy metals including Lead (Pb), Mercury (Hg), Arsenic (As), Chromium (Cr) and Cadmium (Cd) are considered to be highly toxic and hazardous to human health even at trace levels. This leads to the requirement of fast, accurate and reliable techniques for the detection of heavy metal ions. This review presents various electrochemical detection techniques for heavy metal ions those are user friendly, low cost, provides on-site and real time monitoring as compared to other spectroscopic and optical techniques...
March 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28340437/assessment-of-microalga-biofilms-for-simultaneous-remediation-and-biofuel-generation-in-mine-tailings-water
#8
H Palma, E Killoran, M Sheehan, F Berner, K Heimann
Microalgae crops can generate a biochemical profile of high energy density and may be used for remediation of contaminated waste waters. This manuscript presents a laboratory-scale investigation into the potential for growing endemic microalgae biofilms in phosphorus-enriched nickel refinery tailings water, with an emphasis on product potential and the remediation of heavy metals. The dominant species of the consortia was a Chlorella-like microalga. The growth was monitored over time, with a productivity (0...
March 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28340421/cannabis-use-patterns-and-motives-a-comparison-of-younger-middle-aged-and-older-medical-cannabis-dispensary-patients
#9
Nancy A Haug, Claudia B Padula, James E Sottile, Ryan Vandrey, Adrienne J Heinz, Marcel O Bonn-Miller
INTRODUCTION: Medical cannabis is increasingly being used for a variety of health conditions as more states implement legislation permitting medical use of cannabis. Little is known about medical cannabis use patterns and motives among adults across the lifespan. METHODS: The present study examined data collected at a medical cannabis dispensary in San Francisco, California. Participants included 217 medical cannabis patients who were grouped into age-defined cohorts (younger: 18-30, middle-aged: 31-50, and older: 51-72)...
March 9, 2017: Addictive Behaviors
https://www.readbyqxmd.com/read/28340397/quantification-of-hydrocarbon-species-on-surfaces-by-combined-microbalance-ftir
#10
Andrew I McNab, Tom Heinze, Alan J McCue, Davide Dionisi, James A Anderson
Absorption coefficients for the asymmetric stretching modes of CH3 and CH2 groups formed by adsorbing alkyl chained species from the vapour phase onto two different adsorbents; a γ-alumina support material and a supported metal catalyst have been determined using a custom made thermogravimetric-infrared cell. Results show that despite variations in the individually calculated absorption coefficients (ca. ±20%), the ratio of the absorption coefficients (CH2:CH3) remained consistent despite employing adsorbates of varying chain length and functionality, and despite the choice of adsorbents which exhibited different surface areas and light scattering characteristics...
March 16, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28340372/shotgun-metagenomic-sequencing-reveals-freshwater-beach-sands-as-reservoir-of-bacterial-pathogens
#11
Mahi M Mohiuddin, Yasser Salama, Herb E Schellhorn, G Brian Golding
Recreational waters and adjacent beach sands harbor complex microbial communities which may contain human pathogens that cannot be detected by conventional methods. Here, we investigate the diversity of bacterial populations inhabiting four freshwater beaches of the Great Lakes region using shotgun metagenomic sequencing approach. Our analysis suggests that average taxonomic richness and alpha diversity are significantly higher (P < 0.001) in beach sands compared to the corresponding water environments...
March 1, 2017: Water Research
https://www.readbyqxmd.com/read/28340242/insertion-of-miniaturized-cardiac-monitors-outside-the-catheter-operating-room-experience-and-practical-advice
#12
Jan Steffel, David Jay Wright, Harald Schäfer, Tabinda Rashid-Fadel, Thorsten Lewalter
Minor surgical procedures are increasingly being performed as outpatient procedures in settings outside hospital operating rooms (ORs). In electrophysiology, the recent miniaturization of insertable cardiac monitors (ICMs) has enabled the routine insertion of the device as a minimally invasive procedure without the need of a catheter OR. However, a shift to office-based environments for minor surgical procedures is associated with some concerns, particularly with respect to patient- and procedure-related safety in the new setting...
March 17, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28340239/hypertension-in-dialysis-patients-a-consensus-document-by-the-european-renal-and-cardiovascular-medicine-eureca-m-working-group-of-the-european-renal-association-european-dialysis-and-transplant-association-era-edta-and-the-hypertension-and-the-kidney-working
#13
Pantelis A Sarafidis, Alexandre Persu, Rajiv Agarwal, Michel Burnier, Peter de Leeuw, Charles J Ferro, Jean-Michel Halimi, Gunnar H Heine, Michel Jadoul, Faical Jarraya, Mehmet Kanbay, Francesca Mallamaci, Patrick B Mark, Alberto Ortiz, Gianfranco Parati, Roberto Pontremoli, Patrick Rossignol, Luis Ruilope, Patricia Van der Niepen, Raymond Vanholder, Marianne C Verhaar, Andrzej Wiecek, Gregoire Wuerzner, Gérard M London, Carmine Zoccali
In patients with end-stage renal disease (ESRD) treated with haemodialysis or peritoneal dialysis, hypertension is common and often poorly controlled. Blood pressure (BP) recordings obtained before or after haemodialysis display a J- or U-shaped association with cardiovascular events and survival, but this most likely reflects the low accuracy of these measurements and the peculiar haemodynamic setting related to dialysis treatment. Elevated BP detected by home or ambulatory BP monitoring is clearly associated with shorter survival...
March 13, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28340233/increase-in-drug-overdose-deaths-involving-fentanyl-rhode-island-january-2012-march-2014
#14
Melissa C Mercado, Steven A Sumner, M Bridget Spelke, Michele K Bohm, David E Sugerman, Christina Stanley
Objective.:  This study identified sociodemographic, substance use, and multiple opioid prescriber and dispenser risk factors among drug overdose decedents in Rhode Island, in response to an increase in overdose deaths (ODs) involving fentanyl. Methods.:  This cross-sectional investigation comprised all ODs reviewed by Rhode Island's Office of the State Medical Examiners (OSME) during January 2012 to March 2014. Data for 536 decedents were abstracted from OSME's charts, death certificates, toxicology reports, and Prescription Monitoring Program (PMP) databases...
March 6, 2017: Pain Medicine: the Official Journal of the American Academy of Pain Medicine
https://www.readbyqxmd.com/read/28340230/determinants-of-opioid-prescribing-for-nonmalignant-chronic-pain-in-us-outpatient-settings
#15
Rafia S Rasu, Maureen E Knell
Study Objectives.:  Identify prescribing trends for opioid use in non-malignant chronic pain (NMCP) from the National Ambulatory Medical Care Survey (NAMCS). Determine predictors of opioid use based on patient-specific factors. Design.:  This cross-sectional study analyzed NAMCS data from 2000 to 2007. Pain medications prescribed were retrieved using NAMCS drug codes. Multivariate logistic models examined determinants of opioid prescribing among NMCP patients...
March 14, 2017: Pain Medicine: the Official Journal of the American Academy of Pain Medicine
https://www.readbyqxmd.com/read/28340217/establishment-of-reference-interval-for-alkaline-phosphatase-in-healthy-children-of-various-ethnicities-aged-0-12-years
#16
Gu Wanjian, Hu Jie, Ge Liang, Wang Cheng, Xu Tian, Shen Jianjiang, Zhang Chunni
Background: To establish a reference interval of alkaline phosphatase for healthy children of various ethnicities, aged 0 years to 12 years in China. Methods: According to the Clinical and Laboratory Standards Institute guideline C28-A3, blood specimens were collected in the morning after overnight fasting from 502 healthy children to determine the alkaline phosphatase (ALP) level, using the Beckman AU5800 automatic biochemical analyzer. Children were grouped by sex and age...
March 18, 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/28340208/health-care-on-equal-terms-assessing-horizontal-equity-in-health-care-use-in-northern-sweden
#17
Miguel San Sebastián, Paola A Mosquera, Nawi Ng, Per E Gustafsson
The Swedish health care system has successively moved toward increased market-orientation, which has raised concerns as to whether Sweden still offers health on equal terms. To explore this issue, this study aimed (i) to assess if the principles of horizontal equity (equal access for equal need regardless of socio-economic factors) are met in Northern Sweden 2006-14; and (ii) to explore the contribution of different factors to the inequalities in access along the same period. Data came from cross sectional surveys known in 2006, 2010 and 2014 targeting 16-84-year-old residents in the four northern-most counties in Sweden...
March 18, 2017: European Journal of Public Health
https://www.readbyqxmd.com/read/28340206/monitoring-the-quality-of-cardiac-surgery-based-on-three-or-more-surgical-outcomes-using-a-new-variable-life-adjusted-display
#18
Fah Fatt Gan, Xu Tang, Yexin Zhu, Puay Weng Lim
Quality problem or issue: The traditional variable life-adjusted display (VLAD) is a graphical display of the difference between expected and actual cumulative deaths. The VLAD assumes binary outcomes: death within 30 days of an operation or survival beyond 30 days. Full recovery and bedridden for life, for example, are considered the same outcome. This binary classification results in a great loss of information. Initial assessment: Although there are many grades of survival, the binary outcomes are commonly used to classify surgical outcomes...
March 17, 2017: International Journal for Quality in Health Care
https://www.readbyqxmd.com/read/28340168/young-children-s-acute-stress-after-a-burn-injury-disentangling-the-role-of-injury-severity-and-parental-acute-stress
#19
Ann-Christin Haag, Markus A Landolt
 Although injury severity and parental stress are strong predictors of posttraumatic adjustment in young children after burns, little is known about the interplay of these variables. This study aimed at clarifying mediation processes between injury severity and mother's, father's, and young child's acute stress.   Structural equation modeling was used to examine the relationships between injury severity and parental and child acute stress. Parents of 138 burn-injured children (ages 1-4 years) completed standardized questionnaires on average 19 days postinjury...
March 18, 2017: Journal of Pediatric Psychology
https://www.readbyqxmd.com/read/28340160/safety-of-fluoroscopy-guided-transseptal-approach-for-ablation-of-left-sided-arrhythmias
#20
Zviad Matoshvili, Hamid Bastani, Tara Bourke, Frieder Braunschweig, Nikola Drca, Kristjan Gudmundsson, Per Insulander, Anette Jemtrén, Göran Kennebäck, Ott Saluveer, Jonas Schwieler, Jari Tapanainen, Christer Wredlert, Mats Jensen-Urstad
Aims: The transseptal approach is used for left atrial access during the ablation of atrial fibrillation (AF) and other left-sided arrhythmia substrates. Transseptal puncture (TP) is commonly performed with fluoroscopic guidance, contrast injection, and pressure monitoring. In many centres, additional techniques [intracardiac echocardiography (ICE), transoesophageal echocardiography (TEE), radiofrequency needle] are used to facilitate TP but its use adds costs. In this retrospective study, we studied the safety and complication rate when TP was routinely done with fluoroscopic guidance, contrast injection, and pressure monitoring using ICE or TEE only in selected cases...
March 10, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
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