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https://www.readbyqxmd.com/read/28823137/survey-of-genetic-structure-of-geese-using-novel-microsatellite-markers
#1
Fang-Yu Lai, Po-An Tu, Shih-Torng Ding, Min-Jung Lin, Shen-Chang Chang, En-Chung Lin, Ling-Ling Lo, Pei-Hwa Wang
Objective: The aim of this study was to create a set of microsatellite markers with high polymorphism for the genetic monitoring and genetic structure analysis of local goose populations. Methods: Novel microsatellite markers were isolated from the genomic DNA of white Roman geese using short tandem repeated probes. The DNA segments, including short tandem repeats, were tested for their variability among four populations of geese from the Changhua Animal Propagation Station (CAPS)...
August 16, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28823125/the-importance-of-post-thaw-subculture-for-standardizing-cellular-activity-of-fresh-or-cryopreserved-mouse-embryonic-stem-cells
#2
Dong Woo Ko, Jung Ki Yoon, Jong Il Ahn, Myungook Lee, Woo Sub Yang, Ji Yeon Ahn, Jeong Mook Lim
Objective: Much difference in cellular activity was detected between early and late subpassaged embryonic stem cell (ESCs) lines, which can be created by subtle changes in cell manipulation protocol. This study was subsequently examined whether post-thaw subculture of early subpassaged ESC lines could further affect their cellular activity, which results can contribute to standardizing ESC manipulation protocol for minimizing batch difference or data fluctuation. Methods: Fresh (as a control treatment) or cryopreserved F1 hybrid (B6CBAF1) early ESC lines (C57BL/6xCBA) of the 4 (P4) or the 19 passage (P19) were subcultured once, twice or six times under the same condition...
August 16, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28823109/comparison-of-two-home-sleep-testing-devices-with-different-strategies-for-diagnosis-of-osa
#3
Tyler Gumb, Akosua Twumasi, Shahnaz Alimokhtari, Alan Perez, Kathleen Black, David M Rapoport, Jag Sunderram, Indu Ayappa
PURPOSE: Home sleep testing devices are being widely used in diagnosis/screening for obstructive sleep apnea (OSA). We examined differences in OSA metrics obtained from two devices with divergent home monitoring strategies, the Apnea Risk Evaluation System (ARES™, multiple signals plus forehead reflectance oximetry) and the Nonin WristOx2™ (single channel finger transmission pulse oximeter), compared to differences from night-night variability of OSA. METHODS: One hundred fifty-two male/26 female subjects (BMI = 30...
August 19, 2017: Sleep & Breathing, Schlaf & Atmung
https://www.readbyqxmd.com/read/28823065/elevated-serum-estradiol-levels-in-artificial-autologous-frozen-embryo-transfer-cycles-negatively-impact-ongoing-pregnancy-and-live-birth-rates
#4
Rani Fritz, Sangita Jindal, Heather Feil, Erkan Buyuk
PURPOSE: The aim of this study is to evaluate the correlation between serum estradiol (E2) levels during artificial autologous frozen embryo transfer (FET) cycles and ongoing pregnancy/live birth rates (OP/LB). METHODS: A historical cohort study was conducted in an academic setting in order to correlate peak and average estradiol levels with ongoing pregnancy/live birth rates for all autologous artificial frozen embryo transfer cycles performed from 1/2011 to 12/2014...
August 19, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28823035/armored-dna-in-recombinant-baculoviruses-as-controls-in-molecular-genetic-assays
#5
Andrea Freystetter, Christian Paar, Herbert Stekel, Jörg Berg
The widespread use of molecular PCR-based assays in analytical and clinical laboratories brings about the need for test-specific, stable, and reliable external controls (EC) as well as standards and internal amplification controls (IC), in order to arrive at consistent test results. In addition, there is also a growing need to produce and provide stable, well-characterized molecular controls for quality assurance programs. In this study, we describe a novel approach to generate armored double-stranded DNA controls, which are encapsulated in baculovirus (BV) particles of the species Autographa californica multiple nucleopolyhedrovirus...
August 19, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28823029/experience-of-lung-transplantation-in-patients-with-lymphangioleiomyomatosis-at-a-brazilian-reference-centre
#6
Bruno Guedes Baldi, Marcos Naoyuki Samano, Silvia Vidal Campos, Martina Rodrigues de Oliveira, José Eduardo Afonso Junior, Rafael Medeiros Carraro, Ricardo Henrique Oliveira Braga Teixeira, Isabela Pasqualini Minguini, Roni Burlina, Eduardo Zinoni Silva Pato, Carlos Roberto Ribeiro Carvalho, André Nathan Costa
INTRODUCTION: Lung transplantation (LT) is the standard of care for patients with advanced lung diseases, including lymphangioleiomyomatosis (LAM). LAM accounts for only 1% of all LTs performed in the international registry. As a result, the global experience, including the use of mechanistic target of rapamycin (mTOR) inhibitors before and after LT in LAM, is still limited. METHODS: We conducted a retrospective review of all LAM patients who underwent LT at our centre between 2003 and 2016...
August 19, 2017: Lung
https://www.readbyqxmd.com/read/28823028/backbone-resonance-assignments-of-complexes-of-human-voltage-dependent-sodium-channel-nav1-2-iq-motif-peptide-bound-to-apo-calmodulin-and-to-the-c-domain-fragment-of-apo-calmodulin
#7
Ryan Mahling, Adina M Kilpatrick, Madeline A Shea
Human voltage-gated sodium channel NaV1.2 has a single pore-forming α-subunit and two transmembrane β-subunits. Expressed primarily in the brain, NaV1.2 is critical for initiation and propagation of action potentials. Milliseconds after the pore opens, sodium influx is terminated by inactivation processes mediated by regulatory proteins including calmodulin (CaM). Both calcium-free (apo) CaM and calcium-saturated CaM bind tightly to an IQ motif in the C-terminal tail of the α-subunit. Our thermodynamic studies and solution structure (2KXW) of a C-domain fragment of apo (13)C,(15)N- CaM (CaMC) bound to an unlabeled peptide with the sequence of rat NaV1...
August 19, 2017: Biomolecular NMR Assignments
https://www.readbyqxmd.com/read/28823019/sensitive-and-selective-turn-on-chemodosimetric-probes-for-fe-3-based-on-a-skeleton-of-2-hydroxy-1-naphthaldehyde
#8
Ghulam Shabir, Aamer Saeed, Pervaiz Ali Channar, Fayaz Ali Larik, Tanzeela AbdulFatah
A naked-eye colored chemodosimeteric probes 4a-f, based on Schiff-azo dye conjugates were synthesized and characterized. All compounds 4a-f exhibited excellent selectivity and high sensitivity in absorbance toward detection of Fe(3+) in alcoholic solutions under neutral pH conditions. The detection limit of the probe was shown to be up to 0.05 ppm. A simple paper test strip system for the rapid monitoring of Fe(3+) was developed, indicating their convenient use in environmental samples. Electrochemical analysis of metal free conjugates and iron chelated probes confirmed the chelation of ligands...
August 19, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28823018/prevalence-and-nature-of-potential-drug-drug-interactions-among-kidney-transplant-patients-in-a-german-intensive-care-unit
#9
Julia Amkreutz, Alexander Koch, Lukas Buendgens, Anja Muehlfeld, Christian Trautwein, Albrecht Eisert
Background Complex polypharmacotherapy makes kidney transplant patients vulnerable to drug-drug interactions (DDIs). Objective To study prevalence and nature of potential DDIs (pDDIs) in kidney transplant patients. Setting Internal medicine ICU, University Hospital RWTH Aachen. Method In this retrospective observational study, pDDIs were identified in the first week after transplant from 1999 to 2010. Patients aged at least 18 years with prescription of at least two drugs were included. Patients with incomplete data were excluded...
August 19, 2017: International Journal of Clinical Pharmacy
https://www.readbyqxmd.com/read/28823017/near-infrared-spectroscopy-monitoring-during-immediate-transition-after-birth-time-to-obtain-cerebral-tissue-oxygenation
#10
Evelyn Ziehenberger, Berndt Urlesberger, Corinna Binder-Heschl, Bernhard Schwaberger, Nariae Baik-Schneditz, Gerhard Pichler
Feasibility of cerebral tissue oxygenation measurements immediately after birth has been published starting with first values 2 min after birth. Aim of this study was to evaluate, the time periods from birth and from arrival at the resuscitation table to obtain the first cerebral tissue oxygenation values with two different near infrared spectroscopy (NIRS) devices. The present study is an analysis of exploratory parameters of two prospective observational studies. Cerebral tissue oxygen saturation was measured by the NIRO 200NX measuring "cerebral-tissue-oxygenation-index" (cTOI) or the INVOS5100C measuring "cerebral-regional-oxygen-saturation" (crSO2)...
August 19, 2017: Journal of Clinical Monitoring and Computing
https://www.readbyqxmd.com/read/28823005/normocalcaemic-primary-hyperparathyroidism-a-diagnostic-and-therapeutic-algorithm
#11
Joaquín Gómez-Ramírez, Radu Mihai
In recent years, there has been increasing interest in understanding the implications of diagnosing normocalcaemic primary hyperparathyroidism (nPHPT). Many patients hope that nPHPT might explain some of their symptoms, but surgeons hesitate to offer treatment to patients whose calcium levels are normal but whose parathyroid hormone (PTH) levels are elevated in the absence of secondary causes of hyperparathyroidism. This potential new diagnosis is not well understood and may lead to inappropriate investigation and possible unnecessary operations...
August 19, 2017: Langenbeck's Archives of Surgery
https://www.readbyqxmd.com/read/28822956/a-novel-web-support-intervention-to-promote-recovery-following-anterior-cruciate-ligament-reconstruction-a-pilot-randomised-controlled-trial
#12
Pazit Levinger, Karen Hallam, Darren Fraser, Rebecca Pile, Clare Ardern, Brett Moreira, Simon Talbot
BACKGROUND: Self-efficacy is positively associated with adherence behaviours and rehabilitation outcomes following Anterior Cruciate Ligament (ACL) reconstruction. An internet resource can be an effective way to provide information, goal setting, patient monitoring and hence support overall self-management. PURPOSE: This study examined the feasibility of a three month 'internet-based intervention' (mobile-oriented site) to enhance recovery for patients following ACL reconstruction...
June 8, 2017: Physical Therapy in Sport
https://www.readbyqxmd.com/read/28822945/development-of-an-accurate-and-sensitive-method-for-lactate-analysis-in-exhaled-breath-condensate-by-lc-ms-ms
#13
Thomas C Jackson, Yan Victoria Zhang, Patricia J Sime, Richard P Phipps, R Matthew Kottmann
Exhaled breath condensate (EBC) is easily obtained for clinical diagnosis and prognosis for pulmonary diseases and has gained much interest in biomarker discovery research and studies. Lactate, a physiological material, is found in EBC and has been demonstrated to be a potential indicator of chronic obstructive pulmonary disease and other lung diseases. Several assays are available to detect lactate in human body fluids, and yet none is suitable for detecting lactate in EBC. Due to the very low concentration of lactate in EBC and low volume of EBC, it is very important to develop an assay to measure lactate with high sensitivity, accuracy and easy sample processing...
July 24, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28822942/differential-responses-of-soil-microbial-biomass-diversity-and-compositions-to-altitudinal-gradients-depend-on-plant-and-soil-characteristics
#14
Chengjie Ren, Wei Zhang, ZeKun Zhong, Xinhui Han, Gaihe Yang, Yongzhong Feng, Guangxin Ren
Alt'itudinal gradients strongly affect plant biodiversity, but the effects on microbial patterns remain unclear, especially in the large scale. We therefore designed an altitudinal gradient experiment that covered three climate zones to monitor soil microbial community dynamics and to compare those with plant and soil characteristics. Illumina sequencing of the 16S rRNA gene and ITS gene was used to analyze soil microbial (bacterial and fungal) diversity and composition, and fumigation-extraction was used to determine microbial biomass; the plant community metrics (i...
August 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28822936/influence-of-coupled-ocean-atmosphere-phenomena-on-the-greater-horn-of-africa-droughts-and-their-implications
#15
Freddie Mpelasoka, Joseph L Awange, Ayalsew Zerihun
Drought-like humanitarian crises in the Greater Horn of Africa (GHA) are increasing despite recent progress in drought monitoring and prediction efforts. Notwithstanding these efforts, there remain challenges stemming from uncertainty in drought prediction, and the inflexibility and limited buffering capacity of the recurrent impacted systems. The complexity of the interactions of ENSO, IOD, IPO and NAO, arguably remains the main source of uncertainty in drought prediction. To develop practical drought risk parameters that potentially can guide investment strategies and risk-informed planning, this study quantifies, drought characteristics that underpin drought impacts management...
August 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28822930/spatial-and-temporal-patterns-of-pesticide-concentrations-in-streamflow-drainage-and-runoff-in-a-small-swedish-agricultural-catchment
#16
Maria Sandin, Kristin Piikki, Nicholas Jarvis, Mats Larsbo, Kevin Bishop, Jenny Kreuger
A better understanding of the dominant source areas and transport pathways of pesticide losses to surface water is needed for targeting mitigation efforts in a more cost-effective way. To this end, we monitored pesticides in surface water in an agricultural catchment typical of one of the main crop production regions in Sweden. Three small sub-catchments (88-242ha) were selected for water sampling based on a high-resolution digital soil map developed from proximal sensing methods and soil sampling; one sub-catchment had a high proportion of clay soils, another was dominated by coarse sandy soils while the third comprised a mix of soil types...
August 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28822929/enhanced-roadside-drainage-system-for-environmentally-sensitive-areas
#17
William R Trenouth, Bahram Gharabaghi, Hani Farghaly
Stormwater runoff from roadways that encroach upon environmentally sensitive areas (ESAs) is one of the leading causes of degradation in urbanizing watersheds around the world. This is due to toxicity of the pollutant cocktail commonly found in roadway runoff, including heavy metals and sediments, as well as road salts from winter maintenance operations. This paper presents a novel design of an enhanced roadside drainage system (ERDS); an improved roadside drainage system that is intended to protect groundwater recharge zones and sensitive aquatic species in ESAs...
August 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28822914/motor-correlates-of-phantom-limb-pain
#18
Sanne Kikkert, Melvin Mezue, David Henderson Slater, Heidi Johansen-Berg, Irene Tracey, Tamar R Makin
Following amputation, individuals ubiquitously report experiencing lingering sensations of their missing limb. While phantom sensations can be innocuous, they are often manifested as painful. Phantom limb pain (PLP) is notorious for being difficult to monitor and treat. A major challenge in PLP management is the difficulty in assessing PLP symptoms, given the physical absence of the affected body part. Here, we offer a means of quantifying chronic PLP by harnessing the known ability of amputees to voluntarily move their phantom limbs...
July 25, 2017: Cortex; a Journal Devoted to the Study of the Nervous System and Behavior
https://www.readbyqxmd.com/read/28822903/uterine-clinical-findings-fertility-rate-leucocyte-migration-and-cox-2-protein-levels-in-the-endometrial-tissue-of-susceptible-mares-treated-with-platelet-rich-plasma-before-and-after-ai
#19
Lorenzo G Segabinazzi, Aime M Friso, Sebastian B Correal, André M Crespilho, José Antonio Dell'Aqua, Jordi Miró, Frederico O Papa, Marco Antonio Alvarenga
Persistent mating-induced endometritis (PMIE) results in decreased fertility in horses, thereby causing a significant impact in the horse market. Platelet-rich plasma (PRP), a modulator of the inflammatory response, has been largely used in veterinary medicine. Here, we investigated the effects of PRP on uterine inflammation, conception rate, endometrial polymorphonuclear neutrophil (PMN) migration, and COX-2 protein levels in the endometrial tissue. Thirteen PMIE-susceptible mares were used for artificial insemination (AI)...
August 12, 2017: Theriogenology
https://www.readbyqxmd.com/read/28822830/chronic-kidney-disease-in-long-term-survivors-after-allogeneic-hematopoietic-stem-cell-transplantation-retrospective-analysis-at-a-single-institute
#20
Tomoyasu Jo, Yasuyuki Arai, Tadakazu Kondo, Toshiyuki Kitano, Masakatsu Hishizawa, Kouhei Yamashita, Akifumi Takaori-Kondo
The number of patients eligible for allogeneic stem cell transplantation (allo-HSCT) has increased because of improvements in transplantation procedures. Among long-term survivors of allo-HSCT, chronic kidney disease (CKD) is a major cause of morbidity. We retrospectively analyzed the clinical data of 106 consecutive patients with a median age of 43 (17-73) years who had undergone allo-HSCT at our institution between January 2001 and September 2009. Patients who died within 5 years after transplantation or had CKD at the time of transplantation were excluded in this study...
August 16, 2017: Biology of Blood and Marrow Transplantation
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