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https://www.readbyqxmd.com/read/29457050/pelvic-catastrophe-after-elastic-band-ligation-in-an-irradiated-rectum
#1
Inês Pita, Pedro Bastos, Mário Dinis-Ribeiro
Chronic radiation proctopathy is a frequent complication after both pelvic external radiation therapy and brachytherapy and most commonly presents as rectal bleeding. Deep rectal ulcers and fistulae are much rarer and more clinically challenging consequences. We present the case of a 72-year-old male with a history of prostate adenocarcinoma treated with brachytherapy, who was referred to our institution due to a deep painful rectal ulcer refractory to medical treatment. The ulcer presented shortly after a haemorrhoid elastic band ligation and progressed to rectourethral fistulisation despite both faecal and urinary diversion...
January 2018: GE Portuguese Journal of Gastroenterology
https://www.readbyqxmd.com/read/29455001/evolution-and-molecular-mechanism-of-pitas-in-iron-transport-of-streptococcus-species
#2
Kun Cao, Jing Zhang, Xin-Yu Miao, Qiu-Xia Wei, Xin-Lu Zhao, Qing-Yu He, Xuesong Sun
Iron is an essential element for almost all bacteria. The iron ATP-binding cassette (ABC) transporters located on the cell membrane affects bacterial virulence and infection. Although a variety of Fe3+ -transporters have been found in bacteria, their evolutionary processes are rarely studied. Pneumococcal iron ABC transporter (PitA), a highly conserved Fe3+ -transporter in most pathogenic bacteria, influences the capsule formation and virulence of bacteria. However, multiple sequence alignment revealed that PitA is expressed in four different variants in bacteria, and the structural complexity of these variants increases progressively...
February 10, 2018: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29448877/a-new-methodology-for-the-measurement-of-the-root-canal-curvature-and-its-3d-modification-after-instrumentation
#3
Asterios Christodoulou, Georgios Mikrogeorgis, Triantafillia Vouzara, Konstantinos Papachristou, Christos Angelopoulos, Nikolaos Nikolaidis, Ioannis Pitas, Kleoniki Lyroudia
OBJECTIVE: In this study, the three-dimensional (3D) modification of root canal curvature was measured, after the application of Reciproc instrumentation technique, by using cone beam computed tomography (CBCT) imaging and a special algorithm developed for the 3D measurement of the curvature of the root canal. MATERIALS AND METHODS: Thirty extracted upper molars were selected. Digital radiographs for each tooth were taken. Root curvature was measured by using Schneider method and they were divided into three groups, each one consisting of 10 roots, according to their curvature: Group 1 (0°-20°), Group 2 (21°-40°), Group 3 (41°-60°)...
February 15, 2018: Acta Odontologica Scandinavica
https://www.readbyqxmd.com/read/29440764/sperm-selection-by-thermotaxis-improves-icsi-outcome-in-mice
#4
Serafín Pérez-Cerezales, Ricardo Laguna-Barraza, Alejandro Chacón de Castro, María Jesús Sánchez-Calabuig, Esther Cano-Oliva, Francisco Javier de Castro-Pita, Luis Montoro-Buils, Eva Pericuesta, Raúl Fernández-González, Alfonso Gutiérrez-Adán
The ejaculate is a heterogeneous pool of spermatozoa containing only a small physiologically adequate subpopulation for fertilization. As there is no method to isolate this subpopulation, its specific characteristics are unknown. This is one of the main reasons why we lack effective tools to identify male infertility and for the low efficiency of assisted reproductive technologies. The aim of this study was to improve ICSI outcome by sperm selection through thermotaxis. Here we show that a specific subpopulation of mouse and human spermatozoa can be selected in vitro by thermotaxis and that this subpopulation is the one that enters the fallopian tube in mice...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29434446/hsa-mir-202-3p-up-regulated-in-type-1-gastric-neuroendocrine-neoplasms-may-target-dusp1
#5
Dou Dou, Yan-Fen Shi, Qing Liu, Jie Luo, Ji-Xi Liu, Meng Liu, Ying-Ying Liu, Yuan-Liang Li, Xu-Dong Qiu, Huang-Ying Tan
AIM: To detect abnormal microRNA (miRNA) expression in type 1 gastric neuroendocrine neoplasms (g-NENs) and find potential target genes. METHODS: Tumour tissues from patients with type 1 g-NENs were used as experimental samples, and gastric mucosal tissues from the same patients obtained during gastroscopy review after several months were used as control samples. miRNA expression was examined with Agilent human miRNA chips and validated via RT-PCR. Three types of target gene prediction software (TargetScan, PITA, and microRNAorg) were used to predict potential target genes of the differentially expressed miRNAs, and a dual-luciferase reporter assay system was used for verification...
February 7, 2018: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29425208/estimation-of-linkage-disequilibrium-and-analysis-of-genetic-diversity-in-korean-chicken-lines
#6
Dongwon Seo, Doo Ho Lee, Nuri Choi, Pita Sudrajad, Seung-Hwan Lee, Jun-Heon Lee
The development of genetic markers for animal breeding is an effective strategy to reduce the time and cost required to improve economically important traits. To implement genomic selection in the multibreed chicken population of Korea, an understanding of the linkage disequilibrium (LD) status of the target population is essential. In this study, we performed population genetic analyses to investigate LD decay, the effective population size, and breed diversity using 600K high-density single nucleotide polymorphism genotypes of 189 native chickens in 14 lines (including Korean native chicken, imported and adapted purebred and commercial chickens)...
2018: PloS One
https://www.readbyqxmd.com/read/29422852/recurrent-stroke-in-a-young-woman-with-a-single-pulmonary-arteriovenous-fistula-an-unusual-association
#7
Sandra Sousa, Nuno Vasco Costa, Cátia Carmona, Élia Coimbra, Fernando Pita
Introduction: Cryptogenic stroke is present in about 40% of ischemic stroke patients. Extracardiac shunt related to pulmonary arteriovenous fistula (PAVF) could be a rare potential risk factor for embolic stroke. Most PAVFs are multiple, congenital, and associated with hereditary conditions. On the other hand, isolated PAVFs are rare conditions and an uncommon cause of cryptogenic stoke. Case Report: We describe a case of a young woman without history of respiratory diseases or vascular risk factors, who presented with acute onset of transitory aphasia and right hemiplegia...
September 2017: Case Reports in Neurology
https://www.readbyqxmd.com/read/29415837/parenteral-nutrition-associated-cholestasis-and-triglyceridemia-in-surgical-term-and-near-term-neonates-a-pilot-randomized-controlled-trial-of-two-mixed-intravenous-lipid-emulsions
#8
Luís Pereira-da-Silva, Sara Nóbrega, Maria Luísa Rosa, Marta Alves, Ana Pita, Daniel Virella, Ana Luísa Papoila, Micaela Serelha, Gonçalo Cordeiro-Ferreira, Berthold Koletzko
BACKGROUND: Cholestasis is a common complication in infants receiving prolonged parenteral nutrition (PN). We studied the effects of two intravenous lipid emulsions composed with either 30% soybean oil, 30% medium-chain triglycerides (MCT), 25% olive oil, and 15% fish oil (SMOF) or with 50% MCT and 50% soybean oil n-6 (MCT/SOY) on the incidence of cholestasis in surgical term and near-term neonates. METHODS: A single-center, double-blinded, randomized controlled trial compared the incidence of cholestasis using either SMOF or MCT/SOY in neonates born at gestational age ≥34 weeks undergoing major surgery...
December 2017: Clinical Nutrition ESPEN
https://www.readbyqxmd.com/read/29415613/individual-differences-in-episodic-memory-abilities-predict-successful-prospective-memory-output-monitoring
#9
B Hunter Ball, Margarida Pitães, Gene A Brewer
Output monitoring refers to memory for one's previously completed actions. In the context of prospective memory (PM) (e.g., remembering to take medication), failures of output monitoring can result in repetitions and omissions of planned actions (e.g., over- or under-medication). To be successful in output monitoring paradigms, participants must flexibly control attention to detect PM cues as well as engage controlled retrieval of previous actions whenever a particular cue is encountered. The current study examined individual differences in output monitoring abilities in a group of younger adults differing in attention control (AC) and episodic memory (EM) abilities...
February 7, 2018: Memory
https://www.readbyqxmd.com/read/29401783/design-of-a-compact-cmos-compatible-photonic-antenna-by-topological-optimization
#10
Julián L Pita, Ivan Aldaya, Paulo Dainese, Hugo E Hernandez-Figueroa, Lucas H Gabrielli
Photonic antennas are critical in applications such as spectroscopy, photovoltaics, optical communications, holography, and sensors. In most of those applications, metallic antennas have been employed due to their reduced sizes. Nevertheless, compact metallic antennas suffer from high dissipative loss, wavelength-dependent radiation pattern, and they are difficult to integrate with CMOS technology. All-dielectric antennas have been proposed to overcome those disadvantages because, in contrast to metallic ones, they are CMOS-compatible, easier to integrate with typical silicon waveguides, and they generally present a broader wavelength range of operation...
February 5, 2018: Optics Express
https://www.readbyqxmd.com/read/29396899/predictive-factors-of-satisfaction-and-quality-of-life-after-immediate-breast-reconstruction-using-the-breast-q%C3%A2
#11
C Cereijo-Garea, S Pita-Fernández, B Acea-Nebril, R Rey-Villar, A García-Novoa, C Varela-Lamas, S Builes-Ramirez, T Seoane-Pillado, V Balboa-Barreiro
AIMS AND OBJECTIVES: To analyze quality of life and satisfaction after immediate breast reconstruction due to cancer and its determining factors. BACKGROUND: Studying breast reconstruction is important because of its frequency and variability. In addition to the surgical results, it is necessary to analyze the quality of life and patient satisfaction using a specific tool. DESIGN-METHODS: An ambispective design was used (n=101;α=0.05;precision=10%), studying anthropometric, socio-cultural data, Fagerström test, and the BREAST-Q© questionnaire...
February 3, 2018: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/29396461/the-anillin-related-int1-protein-and-the-sep7-septin-collaborate-to-maintain-cellular-ploidy-in-candida-albicans
#12
Sara Orellana-Muñoz, Encarnación Dueñas-Santero, Yolanda Arnáiz-Pita, Francisco Del Rey, Jaime Correa-Bordes, Carlos R Vázquez de Aldana
Variation in cell ploidy is a common feature of Candida albicans clinical isolates that are resistant to the antifungal drug fluconazole. Here, we report that the anillin-related protein Int1 interacts with septins for coupling cytokinesis with nuclear segregation. Loss of Int1 results in a rapid disassembly of duplicated septin rings from the bud neck at the onset of actomyosin ring contraction. Strikingly, this has no major impact on cytokinesis and septum formation. However, Int1 genetically interacts with the Sep7 septin, maintaining the diffusion barrier at the bud neck and guarantying a faithful nuclear segregation...
February 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29384116/anaphylaxis-in-a-food-allergy-outpatient-department-one-year-review
#13
R A Fernandes, F Regateiro, C Pereira, E Faria, J Pita, A Todo-Bom, I Carrapatoso
Background. Anaphylaxis is an acute, potentially fatal, multi-organ allergic reac-tion. Our aim was to characterize the population with food induced anaphylaxis followed over a one-year period. Methods. Retrospective analysis of clinical files of patients with food anaphylaxis observed in our food allergy consultation during 2016. Results. Sixty-two patients were included. In the pediatric group, the implicated allergens were cow's milk, egg and fish and in the adults' group, the commonest allergens were nuts and wheat...
November 30, 2017: European Annals of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29321333/microrna-130a-regulates-both-hcv-and-hbv-replication-through-a-central-metabolic-pathway
#14
Xiaoqiong Duan, Shilin Li, Jacinta A Holmes, Zeng Tu, Yujia Li, Dachuan Cai, Xiao Liu, Wenting Li, Chunhui Yang, Baihai Jiao, Esperance A Schaefer, Dahlene N Fusco, Shadi Salloum, Limin Chen, Wenyu Lin, Raymond T Chung
BACKGROUND: HCV infection has been shown to regulate miR-130a in patient biopsies and in cultured cells. We sought to identify miR-130a target genes and to explore the mechanisms by which miR-130a regulates HCV and HBV replication.METHODS: We used bioinformatics software including miRanda, TargetScan, PITA and RNAhybrid to predict potential miR-130a target genes. miR-130a and its target genes were overexpressed, or knocked down by siRNA or by CRISPR/Cas9 gRNA, respectively. Selected gene mRNAs and their proteins, together with HCV replication in OR6 cells, JFH1 HCV-infected Huh7...
January 10, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29298447/dynamic-influence-of-ph-on-metalloproteinase-activity-in-human-coronal-and-radicular-dentin
#15
Stella F do Amaral, Polliana M C Scaffa, Renata D S Rodrigues, Douglas Nesadal, Marcia M Marques, Fernando N Nogueira, Maria Angela Pita Sobral
The aim of this study was to evaluate the effect of pH on the activation of matrix metalloproteinases (MMPs) of human coronal (CD) and radicular dentin (RD). CD and RD were pulverized to powder, and proteins were extracted with 1% phosphoric acid. The extracted proteins and the demineralized powder were separately incubated in the following solutions: 4-aminophenylmercuric acetate (control) or a buffer solution at different pHs (2.5, 4.5, 5.0, 6.0, and 7.0). After incubation, proteins were separated by electrophoresis to measure MMP activities by zymography...
January 4, 2018: Caries Research
https://www.readbyqxmd.com/read/29289032/phase-synchronization-of-baroclinic-waves-in-a-differentially-heated-rotating-annulus-experiment-subject-to-periodic-forcing-with-a-variable-duty-cycle
#16
P L Read, X Morice-Atkinson, E J Allen, A A Castrejón-Pita
A series of laboratory experiments in a thermally driven, rotating fluid annulus are presented that investigate the onset and characteristics of phase synchronization and frequency entrainment between the intrinsic, chaotic, oscillatory amplitude modulation of travelling baroclinic waves and a periodic modulation of the (axisymmetric) thermal boundary conditions, subject to time-dependent coupling. The time-dependence is in the form of a prescribed duty cycle in which the periodic forcing of the boundary conditions is applied for only a fraction δ of each oscillation...
December 2017: Chaos
https://www.readbyqxmd.com/read/29286557/enzymatic-electrosynthesis-of-alkanes-by-bioelectrocatalytic-decarbonylation-of-fatty-aldehydes
#17
Sofiene Abdellaoui, Florika C Macazo, Rong Cai, Antonio L De Lacey, Marcos Pita, Shelley D Minteer
An enzymatic electrosynthesis system was created by combining an aldehyde deformylating oxygenase (ADO) from cyanobacteria that catalyzes the decarbonylation of fatty aldehydes to alkanes and formic acid with an electrochemical interface. This system is able to produce a range of alkanes (octane to propane) from aldehydes and alcohols. The combination of this bioelectrochemical system with a hydrogenase bioanode yields a H2 /heptanal enzymatic fuel cell (EFC) able to simultaneously generate electrical energy with a maximum current density of 25 μA cm-2 at 0...
December 29, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29284023/growth-phenotype-analysis-of-heme-synthetic-enzymes-in-a-halophilic-archaeon-haloferax-volcanii
#18
Naoki Kosugi, Takuma Araki, Junpei Fujita, Satoru Tanaka, Taketomo Fujiwara
Halophilic euryarchaea lack many of the genes necessary for the protoporphyrin-dependent heme biosynthesis pathway previously identified in animals and plants. Bioinformatic analysis suggested the presence of two heme biosynthetic processes, an Fe-coproporphyrinogen III (coproheme) decarboxylase (ChdC) pathway and an alternative heme biosynthesis (Ahb) pathway, in Haloferax volcanii. PitA is specific to the halophilic archaea and has a unique molecular structure in which the ChdC domain is joined to the antibiotics biosynthesis monooxygenase (ABM)-like domain by a histidine-rich linker sequence...
2017: PloS One
https://www.readbyqxmd.com/read/29283424/integrated-microrna-mrna-analysis-reveals-mir-204-inhibits-cell-proliferation-in-gastric-cancer-by-targeting-cks1b-cxcl1-and-gprc5a
#19
Sirjana Shrestha, Chi-Dung Yang, Hsiao-Chin Hong, Chih-Hung Chou, Chun-San Tai, Men-Yee Chiew, Wen-Liang Chen, Shun-Long Weng, Chung-Chu Chen, Yi-An Chang, Meng-Lin Lee, Wei-Yun Huang, Sheng-Da Hsu, Yi-Chang Chen, Hsien-Da Huang
Gastric cancer (GC) is the second most frequent cause of cancer-related deaths worldwide. MicroRNAs are single-stranded RNA molecules of 21-23 nucleotides that regulate target gene expression through specific base-pairing interactions between miRNA and untranslated regions of targeted mRNAs. In this study, we generated a multistep approach for the integrated analysis of miRNA and mRNA expression. First, both miRNA and mRNA expression profiling datasets in gastric cancer from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) identified 79 and 1042 differentially expressed miRNAs and mRNAs, respectively, in gastric cancer...
December 28, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29276690/effects-of-intragastrically-administered-tepary-bean-lectins-on-digestive-and-immune-organs-preclinical-evaluation
#20
Julia María Alatorre-Cruz, Wendoline Pita-López, Rosa Guadalupe López-Reyes, Roberto Augusto Ferriz-Martínez, Ricardo Cervantes-Jiménez, María de Jesús Guerrero Carrillo, Paola Jazmín Aranda Vargas, Gabriela López-Herrera, Adriana Jheny Rodríguez-Méndez, Aarón Zamora-Arroyo, Héctor Gutiérrez-Sánchez, Tércia Reis de Souza, Alejandro Blanco-Labra, Teresa García-Gasca
Previous work showed that Tepary bean (Phaseolus acutifolius) lectins exhibit differential cytotoxic effects on cancer cell lines by apoptosis induction. In vivo studies using a Tepary bean lectin fraction (TBLF, 50 mg/kg of body weight) after colon cancer induction in rats showed that TBLF inhibited early precancerous lesions without systemic toxicity however, loss of body weight gain and activation of immune cells were observed. In order to know more about the possible adverse effects, we evaluated the administration of TBLF on digestive and immune organs...
2018: Toxicology Reports
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