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https://www.readbyqxmd.com/read/28631172/glutamine-a-major-player-in-nitrogen-catabolite-repression-in-the-yeast-dekkera-bruxellensis
#1
Danielli Batista Bezerra Cajueiro, Denise Castro Parente, Fernanda Cristina Bezerra Leite, Marcos Antonio de Morais Junior, Will de Barros Pita
In the present work we studied the expression of genes from nitrogen central metabolism in the yeast Dekkera bruxellensis and under regulation by the Nitrogen Catabolite Repression mechanism (NCR). These analyses could shed some light on the biological mechanisms involved in the adaptation and survival of this yeast in the sugarcane fermentation process for ethanol production. Nitrogen sources (N-sources) in the form of ammonium, nitrate, glutamate or glutamine were investigated with or without the addition of methionine sulfoximine, which inhibits the activity of the enzyme glutamine synthetase and releases cells from NCR...
June 19, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28619827/architectural-protein-pita-cooperates-with-dctcf-in-organization-of-functional-boundaries-in-bithorax-complex
#2
Olga Kyrchanova, Nikolay Zolotarev, Vladic Mogila, Oksana Maksimenko, Paul Schedl, Pavel Georgiev
Boundaries in the Bithorax Complex (BX-C) of Drosophila delimit autonomous regulatory domains that drive parasegment-specific expression of homeotic genes. BX-C boundaries have two critical functions: they must block crosstalk between adjacent regulatory domains, and at the same time facilitate boundary bypass. The C2H2 zinc finger Pita protein binds to several BX-C boundaries including Fab-7 and Mcp To study Pita functions, we have used a boundary replacement strategy by substituting modified DNAs for the Fab-7 boundary, which is located between the iab-6 and iab-7 regulatory domains...
June 15, 2017: Development
https://www.readbyqxmd.com/read/28602259/assessment-of-condyle-position-fossa-morphology-and-disk-displacement-in-symptomatic-patients
#3
Katharina Alves Rabelo, Saulo Leonardo Sousa Melo, Marianna Guanaes Gomes Torres, Larissa Rangel Peixoto, Paulo Sérgio Flores Campos, Iêda Margarida Crusoé Rocha Rebello, Daniela Pita de Melo
OBJECTIVE: The aim of the study was to determine whether there are any associations between the morphology of the glenoid fossa, articular spaces, and disk displacement by evaluating the magnetic resonance imaging (MRI) scans of symptomatic patients. Gender and age were also evaluated for any effect on the parameters. STUDY DESIGN: MRI scans of 199 temporomandibular joints were assessed for the morphology of the glenoid fossa and articular spaces in both sagittal and coronal views...
April 19, 2017: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/28594864/consistency-in-the-supply-of-larval-fishes-among-coral-reefs-in-french-polynesia
#4
Marc Besson, Camille Gache, Rohan M Brooker, Rakamaly Madi Moussa, Viliame Pita Waqalevu, Moana LeRohellec, Vincent Jaouen, Kévin Peyrusse, Cécile Berthe, Frédéric Bertucci, Hugo Jacob, Christophe Brié, Bruno Wan, René Galzin, David Lecchini
For marine fishes with a bipartite life cycle, pelagic larval dispersal can shape the distribution, connectivity, composition and resilience of adult populations. Numerous studies of larval dispersal, and associated settlement and recruitment processes, have examined the relationship between population connectivity and oceanographic features. However, relatively little is known about spatial and temporal variation in the abundance of larvae settling among different reefs and the extent to which the species assemblage of larvae settling at one location is reflective of the assemblage in neighbouring areas...
2017: PloS One
https://www.readbyqxmd.com/read/28571173/flat-foot-in-a-random-population-and-its-impact-on-quality-of-life-and-functionality
#5
Salvador Pita-Fernandez, Cristina Gonzalez-Martin, Francisco Alonso-Tajes, Teresa Seoane-Pillado, Sonia Pertega-Diaz, Sergio Perez-Garcia, Rocio Seijo-Bestilleiro, Vanesa Balboa-Barreiro
INTRODUCTION: Flat foot is a common deformity in adults. It is characterized by medial rotation and plantar flexion of the talus, eversion of the calcaneus, collapsed medial arch and abduction of the forefoot. AIM: The aim of this study was to determine the prevalence of flat foot and its impact on quality of life, dependence, foot pain, disability and functional limitation among random population of 40-year-old and above. MATERIALS AND METHODS: A cross-sectional study in a random population sample from Cambre (A Coruña-Spain) (n=835) was performed (α =0...
April 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28566333/cdk4-phosphorylation-status-and-a-linked-gene-expression-profile-predict-sensitivity-to-palbociclib
#6
Eric Raspé, Katia Coulonval, Jaime M Pita, Sabine Paternot, Françoise Rothé, Laure Twyffels, Sylvain Brohée, Ligia Craciun, Denis Larsimont, Véronique Kruys, Flavienne Sandras, Isabelle Salmon, Steven Van Laere, Martine Piccart, Michail Ignatiadis, Christos Sotiriou, Pierre P Roger
Cyclin D-CDK4/6 are the first CDK complexes to be activated in the G1 phase in response to oncogenic pathways. The specific CDK4/6 inhibitor PD0332991 (palbociclib) was recently approved by the FDA and EMA for treatment of advanced ER-positive breast tumors. Unfortunately, no reliable predictive tools are available for identifying potentially responsive or insensitive tumors. We had shown that the activating T172 phosphorylation of CDK4 is the central rate-limiting event that initiates the cell cycle decision and signals the presence of active CDK4...
May 31, 2017: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/28559915/combining-results-from-distinct-microrna-target-prediction-tools-enhances-the-performance-of-analyses
#7
Arthur C Oliveira, Luiz A Bovolenta, Pedro G Nachtigall, Marcos E Herkenhoff, Ney Lemke, Danillo Pinhal
Target prediction is generally the first step toward recognition of bona fide microRNA (miRNA)-target interactions in living cells. Several target prediction tools are now available, which use distinct criteria and stringency to provide the best set of candidate targets for a single miRNA or a subset of miRNAs. However, there are many false-negative predictions, and consensus about the optimum strategy to select and use the output information provided by the target prediction tools is lacking. We compared the performance of four tools cited in literature-TargetScan (TS), miRanda-mirSVR (MR), Pita, and RNA22 (R22), and we determined the most effective approach for analyzing target prediction data (individual, union, or intersection)...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28559643/variability-between-clarke-s-angle-and-chippaux-smirak-index-for-the-diagnosis-of-flat-feet
#8
Cristina Gonzalez-Martin, Salvador Pita-Fernandez, Teresa Seoane-Pillado, Beatriz Lopez-Calviño, Sonia Pertega-Diaz, Vicente Gil-Guillen
BACKGROUND: The measurements used in diagnosing biomechanical pathologies vary greatly. The aim of this study was to determine the concordance between Clarke's angle and Chippaux-Smirak index, and to determine the validity of Clarke's angle using the Chippaux-Smirak index as a reference. METHODS: Observational study in a random population sample (n= 1,002) in A Coruña (Spain). After informed patient consent and ethical review approval, a study was conducted of anthropometric variables, Charlson comorbidity score, and podiatric examination (Clarke's angle and Chippaux-Smirak index)...
March 30, 2017: Colombia Médica: CM
https://www.readbyqxmd.com/read/28555551/-economic-crisis-and-portuguese-national-health-service-physicians-findings-from-a-descriptive-study-of-their-perceptions-and-reactions-from-health-care-units-in-the-greater-lisbon-area
#9
Inês Rego, Giuliano Russo, Luzia Gonçalves, Julian Perelman, Pedro Pita Barros
INTRODUCTION: In Europe, scant scientific evidence exists on the impact of economic crisis on physicians. This study aims at understanding the adjustments made by public sector physicians to the changing conditions, and their perceptions on the market for medical services in the Lisbon metropolitan area. MATERIAL AND METHODS: A random sample of 484 physicians from São José Hospital and health center groups in Cascais and Amadora, to explore their perceptions of the economic crisis, and the changes brought to their workload...
April 28, 2017: Acta Médica Portuguesa
https://www.readbyqxmd.com/read/28554045/transparent-mediator-and-membrane-free-enzymatic-fuel-cell-based-on-nanostructured-chemically-modified-indium-tin-oxide-electrodes
#10
Elena González-Arribas, Tim Bobrowski, Chiara Di Bari, Kirill Sliozberg, Roland Ludwig, Miguel D Toscano, Antonio L De Lacey, Marcos Pita, Wolfgang Schuhmann, Sergey Shleev
We detail a mediator- and membrane-free enzymatic glucose/oxygen biofuel cell based on transparent and nanostructured conducting supports. Chemically modified indium tin oxide nanoparticle modified electrodes were used to substantially increase the active surface area without significantly compromising transparency. Two different procedures for surface nanostructuring were employed, viz. spray-coating and drop-coating. The spray-coated biodevice showed superior characteristics as compared to the drop-coated enzymatic fuel cell, as a result of the higher nanostructured surface area as confirmed by electrochemical characterisation, as well as scanning electron and atomic force microscopy...
May 23, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28550833/-scientific-research-policy-for-health-in-portugal-ii-facts-and-suggestions
#11
REVIEW
Cátia Sá Guerreiro, Zulmira Hartz, Luís Sambo, Cláudia Conceição, Gilles Dussault, Giuliano Russo, Miguel Viveiros, Henrique Silveira, Pedro Pita Barros, Paulo Ferrinho
After more than 40 years of democracy and 30 years of European integration, Portugal has bridged the research gap it had previously. However, when compared to global and European research policies, Portugal still has a long way go regarding investment in research and development. Health Research in Portugal has been managed by the Fundação para a Ciência e Tecnologia and the National Health Institute Doctor Ricardo Jorge, and it has not been a political priority, emphasized by the absence of a national scientific research plan for health, resulting in a weak coordination of actors in the field...
March 31, 2017: Acta Médica Portuguesa
https://www.readbyqxmd.com/read/28549896/concordance-among-methods-of-nutritional-assessment-in-patients-included-on-the-waiting-list-for-liver-transplantation
#12
María Teresa García-Rodríguez, Beatriz López-Calviño, María Del Carmen Piñón-Villar, Alejandra Otero-Ferreiro, Francisco Suárez-López, Manuel Gómez-Gutiérrez, Sonia Pértega-Díaz, María Teresa Seoane-Pillado, Salvador Pita-Fernández
BACKGROUND: The aim of the present study was to determine the extent of malnutrition in patients waiting for a liver transplant. The agreement among the methods of nutritional assessment and their diagnostic validity were evaluated. METHODS: Patients on the waiting list for liver transplantation (n = 110) were studied. The variables were: body mass index, analytical parameters, liver disease etiology, and complications. Liver dysfunction was evaluated using the Child-Pugh Scale...
May 23, 2017: Journal of Epidemiology
https://www.readbyqxmd.com/read/28528887/the-cone-technique-for-ebstein-repair-first-cases
#13
Juan-Miguel Gil-Jaurena, María-Teresa González-López, Ana Pita-Fernández, Ramón Pérez-Caballero, Raquel Prieto, Constancio Medrano
No abstract text is available yet for this article.
May 18, 2017: Revista Española de Cardiología
https://www.readbyqxmd.com/read/28527485/delayed-onset-striatal-hands-following-basal-ganglia-anoxic-insult
#14
Sandra Sousa, Fernando Pita
No abstract text is available yet for this article.
February 27, 2017: Acta Médica Portuguesa
https://www.readbyqxmd.com/read/28527482/-scientific-research-policy-for-health-in-portugal-i-european-and-national-environment
#15
REVIEW
Cátia Sá Guerreiro, Zulmira Hartz, Luís Sambo, Cláudia Conceição, Gilles Dussault, Giuliano Russo, Miguel Viveiros, Henrique Silveira, Pedro Pita Barros, Paulo Ferrinho
The global debate on scientific research policy for human health has been led by World Health Organisation with important contributions from other stakeholders such as Council on Health Research for Development, the World Bank and the Global Forum for Health Research. Recently it has been dominated by the thematic agendas of major global financiers. There is a growing interest worldwide in making better use of the evidence resulting from scientific research in health, in the decision-making process regarding health policies, which is fraught with difficulties, as it is the case in Europe...
February 27, 2017: Acta Médica Portuguesa
https://www.readbyqxmd.com/read/28527137/genome-based-identification-of-heterotic-patterns-in-rice
#16
Ulrike Beukert, Zuo Li, Guozheng Liu, Yusheng Zhao, Nadhigade Ramachandra, Vilson Mirdita, Fabiano Pita, Klaus Pillen, Jochen Christoph Reif
BACKGROUND: Hybrid rice breeding facilitates to increase grain yield and yield stability. Long-term success of hybrid breeding depends on the recognition of high-yielding complementary heterotic patterns, which is lacking in crops like rice. RESULT: The main goal of this study was to evaluate the potential and limits to use genomics for establishing heterotic patterns in rice. For this purpose, data of a commercial hybrid rice breeding program targeted to India was analyzed, including 1,960 phenotyped hybrids from three market segments and 262 genotyped parental lines...
December 2017: Rice
https://www.readbyqxmd.com/read/28509134/two-cases-of-atypical-hemolytic-uremic-syndrome-ahus-and-eosinophilic-granulomatosis-with-polyangiitis-egpa-a-possible-relationship
#17
Mercedes Cao, Tamara Ferreiro, Bruna N Leite, Francisco Pita, Luis Bolaños, Francisco Valdés, Angel Alonso, Eduardo Vázquez, Juan Mosquera, María Trigás, Santiago Rodríguez
Atypical hemolytic uremic syndrome (aHUS) is a rare disease characterized by hemolysis, thrombocytopenia, and renal failure. It is related to genetic mutations of the alternative complement pathway and is difficult to differentiate from other prothrombotic microangiopathies. Eosinophilic granulomatosis with polyangiitis (EGPA) (Churg-Strauss syndrome, CSS) is a systemic ANCA-associated vasculitis and a hypereosinophilic disorder where eosinophils seem to induce cell apoptosis and necrosis and therefore, vasculitis...
May 2017: CEN Case Reports
https://www.readbyqxmd.com/read/28500632/altered-expression-of-four-mirna-mir-1238-3p-mir-202-3p-mir-630-and-mir-766-3p-and-their-potential-targets-in-peripheral-blood-from-vitiligo-patients
#18
Zhiwei Shang, Hongwen Li
Vitiligo is an acquired skin disease with pigmentary disorder. Autoimmune destruction of melanocytes is thought to be major factor in the etiology of vitiligo. miRNA-based regulators of gene expression have been reported to play crucial roles in autoimmune disease. Therefore, we attempt to profile the miRNA expressions and predict their potential targets, assessing the biological functions of differentially expressed miRNA. Total RNA was extracted from peripheral blood of vitiligo (experimental group, n = 5) and non-vitiligo (control group, n = 5) age-matched patients...
May 13, 2017: Journal of Dermatology
https://www.readbyqxmd.com/read/28497051/microrna-expression-signature-in-human-calcific-aortic-valve-disease
#19
Hui Wang, Jing Shi, Beibei Li, Qiulian Zhou, Xiangqing Kong, Yihua Bei
Altered microRNA (miRNA, miR) expression has been related to many disease processes; however, the miRNA expression signature in calcific aortic valve disease (CAVD) is unclear. In this study, microarrays were used to determine the miRNA expression signature of tissue samples from healthy individuals (n = 4) and patients with CAVD (n = 4). TargetScan, PITA, and microRNAorg 3-way databases were used to predict the potential target genes. DIANA-miRPath was used to incorporate the aberrant miRNAs into gene pathways...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28477267/chromosomal-localization-of-wolbachia-inserts-in-the-genomes-of-two-subspecies-of-chorthippus-parallelus-forming-a-pyrenean-hybrid-zone
#20
Raquel Toribio-Fernández, José L Bella, Paloma Martínez-Rodríguez, Lisa J Funkhouser-Jones, Seth R Bordenstein, Miguel Pita
Wolbachia are endosymbiotic bacteria of arthropods and nematodes that can manipulate the reproduction of various host organisms to facilitate their own maternal transmission. Moreover, Wolbachia's presence in host germ cells may contribute to the many cases of lateral gene transfer from Wolbachia to host genomes that have been described. A previous study in Chorthippus parallelus, a well-known orthopteroid forming a hybrid zone in the Pyrenees, identified Wolbachia sequences from two major supergroups in the genomes of infected and uninfected Chorthippus parallelus parallelus (Cpp) and Chorthippus parallelus erythropus (Cpe) subspecies...
May 5, 2017: Chromosome Research
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