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https://www.readbyqxmd.com/read/28812976/learning-and-recognition-of-tactile-temporal-sequences-by-mice-and-humans
#1
Michael R Bale, Malamati Bitzidou, Anna Pitas, Leonie S Brebner, Lina Khazim, Stavros T Anagnou, Caitlin D Stevenson, Miguel Maravall
The world around us is replete with stimuli that unfold over time. When we hear an auditory stream like music or speech or scan a texture with our fingertip, physical features in the stimulus are concatenated in a particular order. This temporal patterning is critical to interpreting the stimulus. To explore the capacity of mice and humans to learn tactile sequences, we developed a task in which subjects had to recognise a continuous modulated noise sequence delivered to whiskers or fingertips, defined by its temporal patterning over hundreds of milliseconds...
August 16, 2017: ELife
https://www.readbyqxmd.com/read/28796195/energy-transfer-efficiency-from-zno-nanocrystals-to-eu-3-ions-embedded-in-sio%C3%A2-film-for-emission-at-614-nm
#2
Vivek Mangalam, Kantisara Pita
In this work, we study the energy transfer mechanism from ZnO nanocrystals (ZnO-nc) to Eu(3+) ions by fabricating thin-film samples of ZnO-nc and Eu(3+) ions embedded in a SiO₂ matrix using the low-cost sol-gel technique. The time-resolved photoluminescence (TRPL) measurements from the samples were analyzed to understand the contribution of energy transfer from the various ZnO-nc emission centers to Eu(3+) ions. The decay time obtained from the TRPL measurements was used to calculate the energy transfer efficiencies from the ZnO-nc emission centers, and these results were compared with the energy transfer efficiencies calculated from steady-state photoluminescence emission results...
August 10, 2017: Materials
https://www.readbyqxmd.com/read/28795090/consensus-datasets-of-mouse-mirna-mrna-interactions-from-multiple-online-resources
#3
Bi Zhao, Bin Xue
MiRNAs regulate gene expression by forming base pairing with mRNAs to inhibit the translation of those mRNAs. In many mammalian genomes each, about 2000 miRNAs were found to regulate roughly 60% of all the genes in that genome. Many experimental validations and computational predictions have been done on miRNA:mRNA interactions. Nonetheless, the interactions from different sources are not always consistent. In this study, we integrated multiple online resources, including mirTarBase, TarBase, miRanda, miRDB, PITA, and TargetScan, and developed eleven large-scale datasets containing miRNA:mRNA interactions that are consistent among a specific subgroup of above-mentioned online resources...
October 2017: Data in Brief
https://www.readbyqxmd.com/read/28736993/molecular-characterization-of-streptococcus-pneumoniae-particularly-serotype19a-st320-that-emerged-in-krasnoyarsk-russia
#4
Irina N Protasova, Tsai-Wen Wan, Natalya V Bakhareva, Wei-Chun Hung, Wataru Higuchi, Yasuhisa Iwao, Tatyana A Yelistratova, Natalya A Ilyenkova, Yelena S Sokolovskaya, Galina P Martynova, Ivan V Reva, Galina V Reva, Sergey V Sidorenko, Lee-Jene Teng, Olga V Peryanova, Alla B Salmina, Tatsuo Yamamoto
Streptococcus pneumoniae, a common human pathogen, colonizes the nasopharynx and causes diseases including acute otitis media (AOM). We herein report pneumococcal serotype distributions in children before and after PCV7 vaccination and in patients with pneumococcal disease in Siberian Russia (Krasnoyarsk). Analyses included antimicrobial susceptibility testing, ST typing, a pulsed-field gel electrophoresis analysis, virulence-related surface protein gene (VSG) typing with novel primers, and a structural analysis by scanning electron microscopy...
July 23, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28723933/comparative-repeatome-analysis-on-triatoma-infestans-andean-and-non-andean-lineages-main-vector-of-chagas-disease
#5
Sebastián Pita, Francisco Panzera, Pablo Mora, Jesús Vela, Ángeles Cuadrado, Antonio Sánchez, Teresa Palomeque, Pedro Lorite
Triatoma infestans is the most important Chagas disease vector in South America. Two main evolutionary lineages, named Andean and non-Andean, have been recognized by geographical distribution, phenetic and genetic characteristics. One of the main differences is the genomic size, varying over 30% in their haploid DNA content. Here we realize a genome wide analysis to compare the repetitive genome fraction (repeatome) between both lineages in order to identify the main repetitive DNA changes occurred during T...
2017: PloS One
https://www.readbyqxmd.com/read/28720665/targeted-exome-sequencing-of-krebs-cycle-genes-reveals-candidate-cancer-predisposing-mutations-in-pheochromocytomas-and-paragangliomas
#6
Laura Remacha, Iñaki Comino-Méndez, Susan Richter, Laura Contreras, Maria Currás-Freixes, Guillermo Pita, Rocío Letón, Antonio Galarreta, Rafael Torres-Pérez, Emiliano Honrado, Scherezade Jiménez, Lorena Maestre, Sebastian Moran, Manel Esteller, Jorgina Satrústegui, Graeme Eisenhofer, Mercedes Robledo, Alberto Cascon
Purpose: Mutations in Krebs cycle genes are frequently found in patients with pheochromocytomas/paragangliomas. Disruption of SDH, FH or MDH2 enzymatic activities lead to accumulation of specific metabolites, which give rise to epigenetic changes in the genome that cause a characteristic hypermethylated phenotype. Tumors showing this phenotype, but no alterations in the known predisposing genes, could harbor mutations in other Krebs cycle genes. <p>Experimental Design: We used downregulation and methylation of RBP1, as a marker of a hypermethylation phenotype, to select eleven pheochromocytomas and paragangliomas for targeted exome sequencing of a panel of Krebs cycle-related genes...
July 18, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28709433/erratum-to-toxicity-and-antitumor-potential-of-mesosphaerum-sidifolium-lamiaceae-oil-and-fenchone-its-major-component
#7
Thaísa Leite Rolim, Déborah Ribeiro Pessoa Meireles, Tatianne Mota Batista, Tatyanna Kelvia Gomes de Sousa, Vivianne Mendes Mangueira, Renata Albuquerque de Abrantes, João Carlos Lima Rodrigues Pita, Aline Lira Xavier, Vicente Carlos Oliveira Costa, Leônia Maria Batista, Marcelo Sobral de Abrantes, Marianna Vieira Sobral
No abstract text is available yet for this article.
July 14, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28704362/local-and-global-genetic-diversity-of-protozoan-parasites-spatial-distribution-of-cryptosporidium-and-giardia-genotypes
#8
Juan C Garcia-R, Nigel French, Anthony Pita, Niluka Velathanthiri, Rima Shrestha, David Hayman
Cryptosporidiosis and giardiasis are recognized as significant enteric diseases due to their long-term health effects in humans and their economic impact in agriculture and medical care. Molecular analysis is essential to identify species and genotypes causing these infectious diseases and provides a potential tool for monitoring. This study uses information on species and genetic variants to gain insights into the geographical distribution and spatial patterns of Cryptosporidium and Giardia parasites. Here, we describe the population heterogeneity of genotypic groups within Cryptosporidium and Giardia present in New Zealand using gp60 and gdh markers to compare the observed variation with other countries around the globe...
July 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28684375/complete-mitochondrial-genome-of-triatoma-infestans-hemiptera-reduviidae-triatominae-main-vector-of-chagas-disease
#9
Sebastián Pita, Francisco Panzera, Jesús Vela, Pablo Mora, Teresa Palomeque, Pedro Lorite
The complete mitogenome of Triatoma infestans, the main vector of Chagas disease in South America, was obtained by combining Illumina and Sanger sequencing sequence data. The 17,301bp long genome contains 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs, and a control region. The number, order and orientation of mitochondrial genes are the same as in T. dimidiata, the only Triatominae mt genome published so far. The main differences between both mitogenomes are found in the control region and in the intergenic spacer between the nd1 gene and the tRNA-Ser...
July 3, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28673306/toxicity-and-antitumor-potential-of-mesosphaerum-sidifolium-lamiaceae-oil-and-fenchone-its-major-component
#10
Thaísa Leite Rolim, Déborah Ribeiro Pessoa Meireles, Tatianne Mota Batista, Tatyanna Kelvia Gomes de Sousa, Vivianne Mendes Mangueira, Renata Albuquerque de Abrantes, João Carlos Lima Rodrigues Pita, Aline Lira Xavier, Vicente Carlos Oliveira Costa, Leônia Maria Batista, Marcelo Sobral da Silva, Marianna Vieira Sobral
BACKGROUND: The essential oil from Mesosphaerum sidifolium (L'Hérit.) Harley & J.F.B.Pastore (syn. Hyptis umbrosa), Lamiaceae (EOM), and its major component, have been tested for toxicity and antitumor activity. METHODS: EOM was obtained from aerial parts of M. sidifolium subjected to hydro distillation, and gas chromatography-mass spectrometry was used to characterize the EOM chemical composition. The toxicity was evaluated using haemolysis assay, and acute toxicity and micronucleus tests...
July 3, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28657898/negative-pressure-wound-therapy-for-a-complicated-abdominal-laparotomy-in-neonatal-necrotizing-enterocolitis-a-case-report
#11
Miriam García Gonzalez, Isabel Casal Beloy, Alba Gómez Dovigo, Lorena Miguez Fortes, Teresa Dargallo Carbonell, Salvador Pita-Fernández, Jesús Caramés Bouzán
Necrotizing enterocolitis (NEC) is the most common surgical emergency in neonatal intensive care units, and patients who require surgery have high mortality and morbidity rates. The utility of negative pressure in the management of adults with complicated abdominal wounds has been documented, but there are few reports describing the use of negative pressure wound therapy (NPWT) in children or following neonatal surgery. The case of a 6 day old, 5-weeks premature neonate with NEC is presented. An exploratory midline laparotomy was performed on day 3 of life owing to rectal bleeding and abdominal distension that did not respond to gastric decompression, bowel rest, and intravenous antibiotics...
June 2017: Ostomy/wound Management
https://www.readbyqxmd.com/read/28647427/first-molecular-detection-of-leishmania-infantum-in-sergentomyia-minuta-diptera-psychodidae-in-alentejo-southern-portugal
#12
S Pereira, D Pita-Pereira, T Araujo-Pereira, C Britto, T Costa-Rego, J Ferrolho, M Vilhena, E F Rangel, M L Vilela, M O Afonso
Protozoan parasites, such as Leishmania spp., are the causative agents of many insect-borne infectious diseases with medical and veterinary importance. Leishmaniasis, caused by Leishmania spp., is transmitted by female phlebotomine sand flies. In the Alentejo region of Portugal, located at the north of Algarve, cases of human and canine leishmaniasis caused by Leishmania infantum have been notified. However, no recent studies regarding the sand fly fauna in the region are available. We therefore aimed to explore the phlebotomine sand fly species found in both, Évora and Beja Districts, to gain an insight about the leishmaniasis epidemiology in these areas...
June 21, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28631172/glutamine-a-major-player-in-nitrogen-catabolite-repression-in-the-yeast-dekkera-bruxellensis
#13
Danielli Batista Bezerra Cajueiro, Denise Castro Parente, Fernanda Cristina Bezerra Leite, Marcos Antonio de Morais Junior, Will de Barros Pita
In the present work we studied the expression of genes from nitrogen central metabolism in the yeast Dekkera bruxellensis and under regulation by the Nitrogen Catabolite Repression mechanism (NCR). These analyses could shed some light on the biological mechanisms involved in the adaptation and survival of this yeast in the sugarcane fermentation process for ethanol production. Nitrogen sources (N-sources) in the form of ammonium, nitrate, glutamate or glutamine were investigated with or without the addition of methionine sulfoximine, which inhibits the activity of the enzyme glutamine synthetase and releases cells from NCR...
June 19, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28619827/architectural-protein-pita-cooperates-with-dctcf-in-organization-of-functional-boundaries-in-bithorax-complex
#14
Olga Kyrchanova, Nikolay Zolotarev, Vladic Mogila, Oksana Maksimenko, Paul Schedl, Pavel Georgiev
Boundaries in the Bithorax complex (BX-C) of Drosophila delimit autonomous regulatory domains that drive parasegment-specific expression of homeotic genes. BX-C boundaries have two crucial functions: they must block crosstalk between adjacent regulatory domains and at the same time facilitate boundary bypass. The C2H2 zinc-finger protein Pita binds to several BX-C boundaries, including Fab-7 and Mcp To study Pita functions, we have used a boundary replacement strategy by substituting modified DNAs for the Fab-7 boundary, which is located between the iab-6 and iab-7 regulatory domains...
July 15, 2017: Development
https://www.readbyqxmd.com/read/28602259/assessment-of-condyle-position-fossa-morphology-and-disk-displacement-in-symptomatic-patients
#15
Katharina Alves Rabelo, Saulo Leonardo Sousa Melo, Marianna Guanaes Gomes Torres, Larissa Rangel Peixoto, Paulo Sérgio Flores Campos, Iêda Margarida Crusoé Rocha Rebello, Daniela Pita de Melo
OBJECTIVE: The aim of the study was to determine whether there are any associations between the morphology of the glenoid fossa, articular spaces, and disk displacement by evaluating the magnetic resonance imaging (MRI) scans of symptomatic patients. Gender and age were also evaluated for any effect on the parameters. STUDY DESIGN: MRI scans of 199 temporomandibular joints were assessed for the morphology of the glenoid fossa and articular spaces in both sagittal and coronal views...
April 19, 2017: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/28594864/consistency-in-the-supply-of-larval-fishes-among-coral-reefs-in-french-polynesia
#16
Marc Besson, Camille Gache, Rohan M Brooker, Rakamaly Madi Moussa, Viliame Pita Waqalevu, Moana LeRohellec, Vincent Jaouen, Kévin Peyrusse, Cécile Berthe, Frédéric Bertucci, Hugo Jacob, Christophe Brié, Bruno Wan, René Galzin, David Lecchini
For marine fishes with a bipartite life cycle, pelagic larval dispersal can shape the distribution, connectivity, composition and resilience of adult populations. Numerous studies of larval dispersal, and associated settlement and recruitment processes, have examined the relationship between population connectivity and oceanographic features. However, relatively little is known about spatial and temporal variation in the abundance of larvae settling among different reefs and the extent to which the species assemblage of larvae settling at one location is reflective of the assemblage in neighbouring areas...
2017: PloS One
https://www.readbyqxmd.com/read/28571173/flat-foot-in-a-random-population-and-its-impact-on-quality-of-life-and-functionality
#17
Salvador Pita-Fernandez, Cristina Gonzalez-Martin, Francisco Alonso-Tajes, Teresa Seoane-Pillado, Sonia Pertega-Diaz, Sergio Perez-Garcia, Rocio Seijo-Bestilleiro, Vanesa Balboa-Barreiro
INTRODUCTION: Flat foot is a common deformity in adults. It is characterized by medial rotation and plantar flexion of the talus, eversion of the calcaneus, collapsed medial arch and abduction of the forefoot. AIM: The aim of this study was to determine the prevalence of flat foot and its impact on quality of life, dependence, foot pain, disability and functional limitation among random population of 40-year-old and above. MATERIALS AND METHODS: A cross-sectional study in a random population sample from Cambre (A Coruña-Spain) (n=835) was performed (α =0...
April 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28566333/cdk4-phosphorylation-status-and-a-linked-gene-expression-profile-predict-sensitivity-to-palbociclib
#18
Eric Raspé, Katia Coulonval, Jaime M Pita, Sabine Paternot, Françoise Rothé, Laure Twyffels, Sylvain Brohée, Ligia Craciun, Denis Larsimont, Véronique Kruys, Flavienne Sandras, Isabelle Salmon, Steven Van Laere, Martine Piccart, Michail Ignatiadis, Christos Sotiriou, Pierre P Roger
Cyclin D-CDK4/6 are the first CDK complexes to be activated in the G1 phase in response to oncogenic pathways. The specific CDK4/6 inhibitor PD0332991 (palbociclib) was recently approved by the FDA and EMA for treatment of advanced ER-positive breast tumors. Unfortunately, no reliable predictive tools are available for identifying potentially responsive or insensitive tumors. We had shown that the activating T172 phosphorylation of CDK4 is the central rate-limiting event that initiates the cell cycle decision and signals the presence of active CDK4...
August 2017: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/28559915/combining-results-from-distinct-microrna-target-prediction-tools-enhances-the-performance-of-analyses
#19
Arthur C Oliveira, Luiz A Bovolenta, Pedro G Nachtigall, Marcos E Herkenhoff, Ney Lemke, Danillo Pinhal
Target prediction is generally the first step toward recognition of bona fide microRNA (miRNA)-target interactions in living cells. Several target prediction tools are now available, which use distinct criteria and stringency to provide the best set of candidate targets for a single miRNA or a subset of miRNAs. However, there are many false-negative predictions, and consensus about the optimum strategy to select and use the output information provided by the target prediction tools is lacking. We compared the performance of four tools cited in literature-TargetScan (TS), miRanda-mirSVR (MR), Pita, and RNA22 (R22), and we determined the most effective approach for analyzing target prediction data (individual, union, or intersection)...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28559643/variability-between-clarke-s-angle-and-chippaux-smirak-index-for-the-diagnosis-of-flat-feet
#20
Cristina Gonzalez-Martin, Salvador Pita-Fernandez, Teresa Seoane-Pillado, Beatriz Lopez-Calviño, Sonia Pertega-Diaz, Vicente Gil-Guillen
BACKGROUND: The measurements used in diagnosing biomechanical pathologies vary greatly. The aim of this study was to determine the concordance between Clarke's angle and Chippaux-Smirak index, and to determine the validity of Clarke's angle using the Chippaux-Smirak index as a reference. METHODS: Observational study in a random population sample (n= 1,002) in A Coruña (Spain). After informed patient consent and ethical review approval, a study was conducted of anthropometric variables, Charlson comorbidity score, and podiatric examination (Clarke's angle and Chippaux-Smirak index)...
March 30, 2017: Colombia Médica: CM
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