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https://www.readbyqxmd.com/read/29345961/effects-of-quercetin-kaempferol-and-exogenous-glutathione-on-phospho-and-total-akt-in-3t3-l1-preadipocytes
#1
William Y Boadi, Andrew Lo
Obesity has been reported to be a risk factor for some types of cancer, such as prostate and lung. The AKT or PI3K-AKT is a signal transduction pathway that promotes survival and growth in response to extracellular signals. The aim of this study was to investigate the effects of two flavonoids, quercetin and kaempferol, and exogenous glutathione (GSH) on the expressions of phospho- and total-AKT levels in 3T3-L1 preadipocytes. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) levels were measured in the treated samples and used as the internal standard...
January 18, 2018: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/29342845/anesthetic-preconditioning-as-endogenous-neuroprotection-in-glaucoma
#2
Tsung-Han Chou, Ganeswara Rao Musada, Giovanni Luca Romano, Elizabeth Bolton, Vittorio Porciatti
Blindness in glaucoma is the result of death of Retinal Ganglion Cells (RGCs) and their axons. RGC death is generally preceded by a stage of reversible dysfunction and structural remodeling. Current treatments aimed at reducing intraocular pressure (IOP) are ineffective or incompletely effective in management of the disease. IOP-independent neuroprotection or neuroprotection as adjuvant to IOP lowering in glaucoma remains a challenge as effective agents without side effects have not been identified yet. We show in DBA/2J mice with spontaneous IOP elevation and glaucoma that the lifespan of functional RGCs can be extended by preconditioning RGCs with retrobulbar lidocaine in one eye at four months of age that temporary blocks RGC axonal transport...
January 13, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29339016/expression-of-sperm-plc%C3%AE-and-clinical-outcomes-of-icsi-aoa-in-men-affected-by-globozoospermia-due-to-dpy19l2-deletion
#3
Marziyeh Tavalaee, Michail Nomikos, F Anthony Lai, Mohammad Hossein Nasr-Esfahani
Globozoospermia is characterized by the presence of 100% acrosomeless round-headed spermatozoa in an ejaculate. Failed fertilization after intracytoplasmic sperm injection (ICSI) is commonly reported for globozoospermic couples and can be overcome by artificial oocyte activation (AOA). Phospholipase C zeta (PLCζ) is one of the main sperm factors involved in oocyte activation and its low expression levels mainly account for fertilization failure. Deletion of the DPY19L2 gene is reported as a main genetic cause in over 70% of infertile men with globozoospermia...
December 29, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29337852/reference-gene-selection-for-quantitative-real-time-reverse-transcriptase-pcr-in-annual-ryegrass-lolium-multiflorum-subjected-to-various-abiotic-stresses
#4
Qiuxu Liu, Xiao Qi, Haidong Yan, Linkai Huang, Gang Nie, Xinquan Zhang
To select the most stable reference genes in annual ryegrass (Lolium multiflorum), we studied annual ryegrass leaf tissues exposed to various abiotic stresses by qRT-PCR and selected 11 candidate reference genes, i.e., 18S rRNA, E2, GAPDH, eIF4A, HIS3, SAMDC, TBP-1, Unigene71, Unigene77, Unigene755, and Unigene14912. We then used GeNorm, NormFinder, and BestKeeper to analyze the expression stability of these 11 genes, and used RefFinder to comprehensively rank genes according to stability. Under different stress conditions, the most suitable reference genes for studies of leaf tissues of annual ryegrass were different...
January 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29336483/glucose-metabolism-during-in-vitro-maturation-of-mouse-oocytes-an-study-using-rna-interference
#5
Hong-Li Xie, Shuai Zhu, Jie Zhang, Jing Wen, Hong-Jie Yuan, Liu-Zhu Pan, Ming-Jiu Luo, Jing-He Tan
In previous studies on glucose metabolism during in vitro maturation, intact cumulus-oocyte complexes (COCs) were treated with enzyme inhibitors/activators. Because inhibitors/activators may have non-specificity and/or toxicity, and culture of COCs cannot differentiate whether glucose metabolism of cumulus cells (CCs) or that of the oocyte supports oocyte maturation, results from the previous studies must be verified by silencing genes in either CCs or cumulus-denuded oocytes (DOs). In this study, RNAi was adopted to specify the effects of glucose metabolism in CCs or DOs on oocyte maturation...
January 16, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29331923/cold-regulated-protein-slcor413im1-confers-chilling-stress-tolerance-in-tomato-plants
#6
Xiaocui Ma, Chong Chen, Minmin Yang, Xinchun Dong, Wei Lv, Qingwei Meng
Chilling stress severely affects the growth, development and productivity of crops. Chloroplast, a photosynthesis site, is extremely sensitive to chilling stress. In this study, the functions of a gene encoding a cold-regulated protein (SlCOR413IM1) under chilling stress were investigated using sense and antisense transgenic tomatoes. Under chilling stress, SlCOR413IM1 expression was rapidly induced and the sense lines exhibited better growth state of seedlings and grown tomato plants. Overexpression of SlCOR413IM1 alleviated chilling-induced damage to the chloroplast membrane and structure, whereas suppression of SlCOR413IM1 aggravated the damage to chloroplast...
January 5, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29325696/urinary-mrna-analysis-of-biomarkers-to-epithelial-mesenchymal-transition-of-renal-allograft
#7
Imen Mezni, Pierre Galichon, Mohammed Mongi Bacha, Yi-Chun Xu-Dubois, Imen Sfar, David Buob, Sabrina Benbouzid, Rim Goucha, Yousr Gorgi, Ezzedine Abderrahim, Mondher Ounissi, Karine Dahan, Nacera Ouali, Alexandre Hertig, Isabelle Brocheriou, Aly Raies, Taieb Ben Abdallah, Éric Rondeau
Renal allograft loss is most often a chronic process, irrespective of the mechanism at stake. In this prospective study, we studied the expression of epithelial to mesenchymal transition (EMT) markers vimentin and β-catenin by immunohistochemistry in the surveillance biopsy and measured the mRNA encoding vimentin (VIM), CD45, GAPDH and uroplakin 1a (UPK) by quantitative PCR in urinary cells in 75 renal transplant patients. The aim is to establish a simple screening test for chronic renal allograft dysfunction...
January 8, 2018: Néphrologie & Thérapeutique
https://www.readbyqxmd.com/read/29321553/prediction-of-co-expression-genes-and-integrative-analysis-of-gene-microarray-and-proteomics-profile-of-keshan-disease
#8
Sen Wang, Rui Yan, Bin Wang, Peiru Du, Wuhong Tan, Mikko J Lammi, Xiong Guo
Keshan disease (KD) is a kind of endemic cardiomyopathy which has a high mortality. However, molecular mechanism in the pathogenesis of KD remains poorly understood. Serum samples were collected from 112 KD patients and 112 normal controls. Gene microarray was used to screen differently expressed genes. Genevestigator was applied to forecast co-expression genes of significant gene. iTRAQ proteomics analysis was used to verify significant genes and their co-expression genes. GO, COG, IPA and STRING were applied to undertake function categorization, pathway and network analysis separately...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321347/identification-of-housekeeping-genes-as-references-for-quantitative-real-time-rt-pcr-analysis-in-misgurnus-anguillicaudatus
#9
Xiaohua Xia, Weiran Huo, Ruyan Wan, Xiaopei Xia, Qiyan Du, Zhongjie Chang
Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) is a well-known method to quantify gene expression by comparing with the reference genes. Generally, housekeeping genes were set as references, as for their stable expression in varying conditions. Here, we try to evaluate few of such genes to identify suitable housekeeping genes as references for qRT-PCR analysis of gene expression in Misgurnus anguillicaudatus. This study evaluated the expression of four commonly used housekeeping genes, i...
December 2017: Journal of Genetics
https://www.readbyqxmd.com/read/29298351/functional-consequences-of-piceatannol-binding-to-glyceraldehyde-3-phosphate-dehydrogenase
#10
Joanna Gerszon, Eligiusz Serafin, Adam Buczkowski, Sylwia Michlewska, Jakub Antoni Bielnicki, Aleksandra Rodacka
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key redox-sensitive proteins whose activity is largely affected by oxidative modifications at its highly reactive cysteine residue in the enzyme's active site (Cys149). Prolonged exposure to oxidative stress may cause, inter alia, the formation of intermolecular disulfide bonds leading to accumulation of GAPDH aggregates and ultimately to cell death. Recently these anomalies have been linked with the pathogenesis of Alzheimer's disease. Novel evidences indicate that low molecular compounds may be effective inhibitors potentially preventing the GAPDH translocation to the nucleus, and inhibiting or slowing down its aggregation and oligomerization...
2018: PloS One
https://www.readbyqxmd.com/read/29289615/the-role-of-resveratrol-and-melatonin-in-the-nitric-oxide-and-its-oxidation-products-mediated-functional-and-structural-modifications-of-two-glycolytic-enzymes-gapdh-and-ldh
#11
Joanna Strumillo, Katarzyna E Nowak, Anita Krokosz, Aleksandra Rodacka, Mieczyslaw Puchala, Grzegorz Bartosz
No abstract text is available yet for this article.
December 28, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29286140/identification-of-key-genes-implicated-in-the-suppressive-function-of-human-foxp3-cd25-cd4-regulatory-t-cells-through-the-analysis-of-time%C3%A2-series-data
#12
Xiaofeng Bai, Hua Shi, Mingxi Yang, Yuanlin Wang, Zhaolin Sun, Shuxiong Xu
Human forkhead box P3 (FOXP3)+ cluster of differentiation (CD)25+CD4+ regulatory T cells (Tregs) are a type of T cell that express CD4, CD25 and FOXP3, which are critical for maintaining immune homeostasis. The present study aimed to determine the mechanisms underlying Treg function. The GSE11292 dataset was downloaded from the Gene Expression Omnibus, which included data from Treg cells at 19 time points (0‑360 min) with an equal interval of 20 min, and corresponding repeated samples. However, data for Treg cells at time point 120 min were missing...
December 29, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29277131/direct-comparison-of-vasectomized-males-and-genetically-sterile-gapdhs-knockout-males-for-the-induction-of-pseudopregnancy-in-mice
#13
Wiebke Garrels, Dirk Wedekind, Isabell Wittur, Ulrike Freischmidt, Dirk Korthaus, Thomas Rülicke, Martina Dorsch
The laboratory mouse is the most used animal model in biomedical research. Several artificial reproductive techniques, such as revitalization of cryopreserved strains, rederivation after hygienic contaminations and the production of transgenic mouse models, require the transfer of preimplantation embryos to surrogate mothers. Pseudopregnancy is essential in recipient females and is induced by mating with sterile males. Commonly, surgically vasectomized males are used for this purpose. As an alternative, genetically modified mouse strains have been identified, in which homozygous infertile males are sexually active...
January 1, 2017: Laboratory Animals
https://www.readbyqxmd.com/read/29261292/haloacetic-acid-water-disinfection-by-products-affect-pyruvate-dehydrogenase-activity-and-disrupt-cellular-metabolism
#14
Azra Dad, Clara Hyunhee Jeong, Elizabeth D Wagner, Michael J Plewa
The disinfection of drinking water has been a major public health achievement. However, haloacetic acids (HAAs), generated as by-products of water disinfection, are cytotoxic, genotoxic, mutagenic, carcinogenic and teratogenic. Previous studies of monoHAA-induced genotoxicity and cell stress demonstrated that the toxicity was due to inhibition of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) leading to disruption of cellular metabolism and energy homeostasis. DiHAAs and triHAAs are also produced during water disinfection, and whether they share mechanisms of action with monoHAAs is unknown...
December 20, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29258442/a-tissue-based-approach-to-selection-of-reference-genes-for-quantitative-real-time-pcr-in-a-sheep-osteoporosis-model
#15
Felix Schulze, Deeksha Malhan, Thaqif El Khassawna, Christian Heiss, Anja Seckinger, Dirk Hose, Angela Rösen-Wolff
BACKGROUND: In order to better understand the multifactorial nature of osteoporosis, animal models are utilized and compared to healthy controls. Female sheep are well established as a model for osteoporosis induced by ovariectomy, calcium and vitamin D low diet, application of steroids, or a combination of these treatments. Transcriptional studies can be performed by applying quantitative real time PCR (RT-qPCR). RT-qPCR estimates mRNA-levels of target genes in relation to reference genes...
December 19, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29249019/acrolein-toxicity-at-advanced-age-present-and-future
#16
REVIEW
Kazuei Igarashi, Takeshi Uemura, Keiko Kashiwagi
It is thought that tissue damage at advanced age is mainly caused by ROS (reactive oxygen species, O2-, H2O2, and ·OH). However, it was found that acrolein (CH2=CH-CHO) is more toxic than ROS, and is mainly produced from spermine (SPM), one of the polyamines, rather than from unsaturated fatty acids. Significant amounts of SPM are present normally as SPM-ribosome complexes, and contribute to protein synthesis. However, SPM was released from ribosomes due to the degradation of ribosomal RNA by ·OH or the binding of Ca2+ to ribosomes, and acrolein was produced from free SPM by polyamine oxidases, particularly by SPM oxidase...
December 16, 2017: Amino Acids
https://www.readbyqxmd.com/read/29243990/quantification-of-plasma-cell-free-circulating-dna-at-different-stages-of-colorectal-cancer
#17
Shahram Agah, Abolfazl Akbari, Atefeh Talebi, Mohsen Masoudi, Arash Sarveazad, Alireza Mirzaei, Farinaz Nazmi
Cell-free circulating DNAs (cfcDNAs) have been recognized as promising biomarkers for a number of cancers. This study aimed to quantify the cfcDNA in colorectal cancer to assess its potential value as biomarker. Quantification of baseline cfcDNA was determined as the amount of free glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in plasma, using quantitative real-time polymerase chain reaction (PCR). The calculated area under the curve (AUC) of receiver operating characteristic (ROC) for cfcDNA was 0.875 (95% CI, 0...
November 26, 2017: Cancer Investigation
https://www.readbyqxmd.com/read/29230202/development-of-a-novel-escherichia-coli-kocuria-shuttle-vector-using-the-cryptic-pkpal3-plasmid-from-k-palustris-ipufs-1-and-its-utilization-in-producing-enantiopure-s-styrene-oxide
#18
Hiroshi Toda, Nobuya Itoh
The novel cryptic pKPAL3 plasmid was isolated from the Gram-positive microorganism Kocuria palustris IPUFS-1 and characterized in detail. pKPAL3 is a circular plasmid that is 4,443 bp in length. Open reading frame (ORF) and homology search analyses indicated that pKPAL3 possesses four ORFs; however, there were no replication protein coding genes predicted in the plasmid. Instead, there were two nucleotide sequence regions that showed significant identities with untranslated regions of K. rhizophila DC2201 (NBRC 103217) genomic sequences, and these sequences were essential for autonomous replication of pKPAL3 in Kocuria cells...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29226720/memantine-induces-apoptosis-and-inhibits-cell-cycle-progression-in-lncap-prostate-cancer-cells
#19
G Albayrak, E Konac, A U Dikmen, C Y Bilen
Deregulated cancer cell metabolism plays an important role in cancer progression. Cancer cell metabolism has been in the centre of attention in therapeutical cancer cell targeting. Repurposed chemical agents, such as metformin and aspirin, have been studied extensively as preventive and therapeutic agents. Metformin is Food and Drug administration (FDA)-approved antidiabetic drug cheaper than other chemotherapeutic agents that were shown to have anticancer effects. Memantine is an FDA-approved Alzheimer's drug...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/29219623/usual-normalization-strategies-for-gene-expression-studies-impair-the-detection-and-analysis-of-circadian-patterns
#20
Diego de Siqueira Figueredo, Mayara Rodrigues Barbosa, Daniel Gomes Coimbra, José Luiz Araújo Dos Santos, Ellyda Fernanda Lopes Costa, Bruna Del Vechio Koike, Magna Suzana Alexandre Moreira, Tiago Gomes de Andrade
Recent studies have shown that transcriptomes from different tissues present circadian oscillations. Therefore, the endogenous variation of total RNA should be considered as a potential bias in circadian studies of gene expression. However, normalization strategies generally include the equalization of total RNA concentration between samples prior to cDNA synthesis. Moreover, endogenous housekeeping genes (HKGs) frequently used for data normalization may exhibit circadian variation and distort experimental results if not detected or considered...
December 8, 2017: Chronobiology International
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