keyword
MENU ▼
Read by QxMD icon Read
search

GAPDH

keyword
https://www.readbyqxmd.com/read/28812429/metastatic-colon-adenocarcinoma-has-a-significantly-elevated-expression-of-il-10-compared-to-primary-colon-adenocarcinoma-tumors
#1
Michelle H Townsend, Abigail M Felsted, Stephen R Piccolo, Richard A Robison, Kim L O'Neill
Classical anti-inflammatory cytokines are known to play a role in both cancer progression as well as cancer elimination. We evaluated the anti-inflammatory cytokines IL-10 and TGF-β in patients with colon adenocarcinoma and metastatic colon adenocarcinoma utilizing immunohistochemical assays to determine the expression of the cytokines between various malignant tissues. We found tissues stained with TGF-β showed no significant upregulation within malignant tumors when compared to normal tissue controls. We observed high levels of TGF-β presence in most tissues similar to GAPDH expression...
August 16, 2017: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/28810828/suitable-reference-gene-for-quantitative-real-time-pcr-analysis-of-gene-expression-in-gonadal-tissues-of-minnow-puntius-sophore-under-high-temperature-stress
#2
Arabinda Mahanty, Gopal Krishna Purohit, Sasmita Mohanty, Nihar Ranjan Nayak, Bimal Prasanna Mohanty
BACKGROUND: High ambient temperature is known to affect fish gonadal development and physiology in a variety of ways depending on the severity and duration of exposure; however, the underlying molecular mechanisms are poorly understood. Gonadal gene expression influence the gonadal development, physiology and the quality of egg/sperm produced in teleosts and the mechanistic understanding of spatio-temporal changes in the gonadal gene expression could be instrumental in controlling the fate of egg/sperm and the quality of seed produced...
August 15, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28807828/identification-of-novel-direct-protein-protein-interactions-by-irradiating-living-cells-with-femtosecond-uv-laser-pulses
#3
Francesco Itri, Daria Maria Monti, Marco Chino, Roberto Vinciguerra, Carlo Altucci, Angela Lombardi, Renata Piccoli, Leila Birolo, Angela Arciello
The identification of protein-protein interaction networks in living cells is becoming increasingly fundamental to elucidate main biological processes and to understand disease molecular bases on a system-wide level. We recently described a method (LUCK, Laser UV Cross-linKing) to cross-link interacting protein surfaces in living cells by UV laser irradiation. By using this innovative methodology, that does not require any protein modification or cell engineering, here we demonstrate that, upon UV laser irradiation of HeLa cells, a direct interaction between GAPDH and alpha-enolase was "frozen" by a cross-linking event...
August 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28807016/endogenous-controls-of-gene-expression-in-n-methyl-n-nitrosourea-induced-t-cell-lymphoma-in-p53-deficient-mice
#4
Xi Wu, Susu Liu, Jianjun Lyu, Shuya Zhou, Yanwei Yang, Chenfei Wang, Wenda Gu, Qin Zuo, Baowen Li, Changfa Fan
BACKGROUND: Real-time polymerase chain reaction (PCR) has become an increasingly important technique for gene expression profiling because it can provide insights into complex biological and pathological processes and be used to predict disease or treatment outcomes. Although normalized data are necessary for an accurate estimation of mRNA expression levels, several pieces of evidence suggest that the expression of so-called housekeeping genes is not stable. This study aimed to validate reference genes for the normalization of real-time PCR in an N-methyl-N-nitrosourea (MNU)-induced T-cell lymphoma mouse model...
August 14, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28803544/overcoming-qrt-pcr-interference-by-select-carbon-nanotubes-in-assessments-of-gene-expression
#5
Sara T Humes, Shannon Hentschel, Candice M Lavelle, L Cody Smith, John A Lednicky, Navid B Saleh, Tara Sabo-Attwood
Nanomaterials (NMs) of various types, including carbon nanotubes (CNTs), can interfere with standard quantitative real-time PCR (qRT-PCR) assays, resulting in inaccurate gene expression measurements; however, the precise step in the qRT-PCR pipeline where this interference occurs has not been well described. Here, we investigated where in the process surface-oxidized multi-walled CNTs (oxMWNTs) inhibited qRT-PCR measurement of the expression of the housekeeping gene GAPDH and explored several strategies to minimize such inhibition...
August 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/28798909/analysis-of-urine-composition-in-type-ii-diabetic-mice-after-intervention-therapy-using-holothurian-polypeptides
#6
Yanyan Li, Jiajie Xu, Xiurong Su
Hydrolysates and peptide fractions (PF) obtained from sea cucumber with commercial enzyme were studied on the hyperglycemic and renal protective effects on db/db rats using urine metabolomics. Compared with the control group the polypeptides from the two species could significantly reduce the urine glucose and urea. We also tried to address the compositions of highly expressed urinary proteins using a proteomics approach. They were serum albumins, AMBP proteins, negative trypsin, elastase, and urinary protein, GAPDH, a receptor of urokinase-type plasminogen activator (uPAR), and Ig kappa chain C region...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28780604/mir-338-5p-promotes-glioma-cell-invasion-by-regulating-tshz3-and-mmp2
#7
Yanyan Li, Yulun Huang, Zhenyu Qi, Ting Sun, Youxin Zhou
This study was designed to examine differential expression of miR-338-5p in gliomas and the role of miR-338-5p in glioma cell invasion via its potential target gene TSHZ3 encoding Teashirt zinc finger homobox 3, predicted by bioinformatics, and matrix metallopeptidase 2 (MMP2), the key pro-invasive protease overexpressed in gliomas. Quantitative real-time reverse transcription PCR (qRT-PCR) and Spearman correlation analysis were used to determine differential expressions of miR-338-5p and TSHZ3 in astrocytic gliomas of different grades (n = 35) and glioblastoma cell lines (U87 and U251) in comparison to non-neoplastic brain (NNB) tissues (n = 6)...
August 5, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28777335/systematic-selection-of-reference-genes-for-the-normalization-of-circulating-rna-transcripts-in-pregnant-women-based-on-rna-seq-data
#8
Stephen S C Chim, Karen K W Wong, Claire Y L Chung, Stephanie K W Lam, Jamie S L Kwok, Chit-Ying Lai, Yvonne K Y Cheng, Annie S Y Hui, Meng Meng, Oi-Ka Chan, Stephen K W Tsui, Keun-Young Lee, Ting-Fung Chan, Tak-Yeung Leung
RNA transcripts circulating in peripheral blood represent an important source of non-invasive biomarkers. To accurately quantify the levels of circulating transcripts, one needs to normalize the data with internal control reference genes, which are detected at relatively constant levels across blood samples. A few reference gene candidates have to be selected from transcriptome data before the validation of their stable expression by reverse-transcription quantitative polymerase chain reaction. However, there is a lack of transcriptome, let alone whole-transcriptome, data from maternal blood...
August 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28776671/%C3%AE-asarone-inhibited-cell-growth-and-promoted-autophagy-via-p53-bcl-2-bclin-1-and-p53-ampk-mtor-pathways-in-human-glioma-u251-cells
#9
Nanbu Wang, Qinxin Zhang, Laiyu Luo, Baile Ning, Yongqi Fang
Glioma is the most common type of primary brain tumor and has an undesirable prognosis. Autophagy plays an important role in cancer therapy, but it's effect is still not definite. P53 is an important tumor suppressor gene and protein that is closely to autophagy. Our aim was to study the effect of β-asarone on inhibiting cell proliferation in human glioma U251 cells and to detect the effect of the inhibition on autophagy through the P53 signal pathway. For cell growth, the cells were divided into four groups: the model, β-asarone, temozolomide (TMZ) and co-administration groups...
August 4, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28769877/protective-efficacy-of-coccidial-common-antigen-glyceraldehyde-3-phosphate-dehydrogenase-gapdh-against-challenge-with-three-eimeria-species
#10
Lu Tian, Wenyu Li, Xinmei Huang, Di Tian, Jianhua Liu, Xinchao Yang, Lianrui Liu, Ruofeng Yan, Lixin Xu, Xiangrui Li, Xiaokai Song
Coccidiosis is an intestinal disorder of poultry and often caused by simultaneous infections of several Eimeria species. GAPDH is one of the immunogenic common antigens among Eimeria tenella, E. acervulina, and E. maxima identified in our previous study. The present study was performed to further evaluate its immunogenicity and protective efficacy. The genes of GAPDH cloned from E. acervulina and E. maxima were named as EaGAPDH and EmGAPDH, respectively. The immunogenicity of recombinant proteins of EaGAPDH and EmGAPDH were analyzed by Western blot...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28765619/reference-gene-selection-to-determine-differences-in-mitochondrial-gene-expressions-in-phosphine-susceptible-and-phosphine-resistant-strains-of-cryptolestes-ferrugineus-using-qrt-pcr
#11
Pei-An Tang, Jin-Yan Duan, Hai-Jing Wu, Xing-Rong Ju, Ming-Long Yuan
Cryptolestes ferrugineus is a serious pest of stored grain and has developed high levels of resistance to phosphine fumigants in many countries. Measuring differences in expression levels of certain 'resistant' genes by quantitative real-time PCR (qRT-PCR) may provide insights into molecular mechanisms underlying resistance to phosphine in C. ferrugineus, but reliable qRT-PCR results depend on suitable reference genes (RGs). We evaluated the stability of nine candidate RGs across different developmental stages and phosphine strains of C...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28763741/expression-stability-and-selection-of-optimal-reference-genes-for-gene-expression-normalization-in-early-life-stage-rainbow-trout-exposed-to-cadmium-and-copper
#12
Kamran Shekh, Song Tang, Som Niyogi, Markus Hecker
Gene expression analysis represents a powerful approach to characterize the specific mechanisms by which contaminants interact with organisms. One of the key considerations when conducting gene expression analyses using quantitative real-time reverse transcription-polymerase chain reaction (qPCR) is the selection of appropriate reference genes, which is often overlooked. Specifically, to reach meaningful conclusions when using relative quantification approaches, expression levels of reference genes must be highly stable and cannot vary as a function of experimental conditions...
September 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28763106/identification-of-proteome-antigen-protein-and-antigen-membrane-protein-from-spiroplasma-eriocheiris
#13
Yuhan Liu, Yinbin Xu, Shuang Li, Xuechuan Xu, Qi Gao, Meijun Yuan, Wei Gu, Wen Wang, Qingguo Meng
Spiroplasma eriocheiris, which causes tremor disease of Chinese mitten crab Eriocheir sinensis, has led to catastrophic economic loses in aquaculture. Immunoproteomics, a new scientific technique combining proteomics and immunological analytical methods, provided the direction of our research on S. eriocheiris. The aim of our study was to identify the proteome, antigen proteins and antigen membrane proteins of S. eriocheiris. A total of 780 S. eriocheiris proteins were identified by LC-MS/MS technique. Based on immunoproteomics, 51 proteins and 7 proteins in S...
August 1, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28757123/molecular-and-biochemical-characterisation-and-recognition-by-the-immune-host-of-the-glyceraldehyde-3-phosphate-dehydrogenase-gapdh-of-the-abomasal-nematode-parasite-teladorsagia-circumcincta
#14
S Umair, C L G Bouchet, J S Knight, A Pernthaner, H V Simpson
A 1023 bp full length cDNA encoding Teladorsagia circumcincta GAPDH (TeciGAPDH) was cloned, expressed in Escherichia coli and the recombinant protein purified and its kinetic properties determined. A phylogenetic tree was constructed using helminth GAPDH sequences. The predicted protein consisted of 341 amino acids and was present as a single band of about 38 kDa on SDS-PAGE. Multiple alignments of the protein sequence of TeciGAPDH with homologues from other helminths showed that the greatest similarity (93%) to the GAPDH of Haemonchus contortus and Dictyocaulus viviparus, 82-86% similarity to the other nematode sequences and 68-71% similarity to cestode and trematode enzymes...
July 27, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28756326/bovine-serum-albumin-and-skim-milk-improve-boar-sperm-motility-by-enhancing-energy-metabolism-and-protein-modifications-during-liquid-storage-at-17%C3%A2-%C3%A2-c
#15
Jieli Fu, Yuhua Li, Lirui Wang, Linqing Zhen, Qiangzhen Yang, Peifei Li, Xinhong Li
Both bovine serum albumin (BSA) and skim-milk have been reported to improve sperm quality, primarily by enhancing sperm motility, but the underlying molecular mechanism remains unknown. In this study, boar semen samples were collected and diluted with Androstar(®) Plus extender containing different concentrations (0, 2, 4 g/l) of BSA and skim-milk. On days 0, 3, 5 and 7, the sperm motility parameters were determined using computer-assisted sperm analysis (CASA), and the ATP concentrations, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity and mitochondrial membrane potential were evaluated using commercial kits...
July 21, 2017: Theriogenology
https://www.readbyqxmd.com/read/28753580/gnp-gapdh1-22-nanovaccines-prevent-neonatal-listeriosis-by-blocking-microglial-apoptosis-and-bacterial-dissemination
#16
Ricardo Calderon-Gonzalez, Elisabet Frande-Cabanes, Hector Teran-Navarro, José María Marimon, Javier Freire, David Salcines-Cuevas, M Carmen Fariñas, Claudia Gonzalez-Rico, Marco Marradi, Isabel Garcia, Mirian Alkorta-Gurrutxaga, Aida San Nicolas-Gomez, Ana Castañeda-San Pedro, Sonsoles Yañez-Diaz, Soledad Penades, Carmen Punzon, Javier Gomez-Roman, Fernando Rivera, Manuel Fresno, Carmen Alvarez-Dominguez
Clinical cases of neonatal listeriosis are associated with brain disease and fetal loss due to complications in early or late pregnancy, which suggests that microglial function is altered. This is believed to be the first study to link microglial apoptosis with neonatal listeriosis and listeriosis-associated brain disease, and to propose a new nanovaccine formulation that reverses all effects of listeriosis and confers Listeria monocytogenes (LM)-specific immunity. We examined clinical cases of neonatal listeriosis in 2013-2015 and defined two useful prognostic immune biomarkers to design listeriosis vaccines: high anti-GAPDH1-22 titres and tumor necrosis factor (TNF)/interleukin (IL)-6 ratios...
July 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/28753256/a-novel-moonlight-function-of-glyceraldehyde-3-phosphate-dehydrogenase-gapdh-for-immunomodulation
#17
Toshiaki Nakano, Shigeru Goto, Yuki Takaoka, Hui-Peng Tseng, Takashi Fujimura, Seiji Kawamoto, Kazuhisa Ono, Chao-Long Chen
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an energy metabolism-related enzyme, which generates NADH in glycolysis. Our previous study revealed a novel role of exogenous GAPDH in the amelioration of lipopolysaccharide (LPS)-induced sepsis-related, severe acute lung injury (ALI) in mice. Here, we show the effect of extracellular GAPDH on the physiological functions of macrophages, which play an important role in the onset of sepsis and ALI. GAPDH has no effect on cell viability, while it strongly suppressed cell adhesion, spreading, and phagocytic function of LPS-stimulated macrophages...
July 28, 2017: BioFactors
https://www.readbyqxmd.com/read/28746411/selection-and-validation-of-reference-genes-for-quantitative-real-time-pcr-analysis-under-different-experimental-conditions-in-the-leafminer-liriomyza-trifolii-diptera-agromyzidae
#18
Ya-Wen Chang, Jing-Yun Chen, Ming-Xing Lu, Yuan Gao, Zi-Hua Tian, Wei-Rong Gong, Wei Zhu, Yu-Zhou Du
Liriomyza trifolii is a highly-invasive leafmining insect that causes significant damage to vegetables and horticultural crops worldwide. Relatively few studies have quantified gene expression in L. trifolii using real-time quantitative PCR (RT-qPCR), which is a reliable and sensitive technique for measuring gene expression. RT-qPCR requires the selection of reference genes to normalize gene expression data and control for internal differences between samples. In this study, nine housekeeping genes from L. trifolii were selected for their suitability in normalizing gene expression using geNorm, Normfinder, BestKeeper, the ΔCt method and RefFinder...
2017: PloS One
https://www.readbyqxmd.com/read/28734275/low-level-of-autophagy-related-gene-10-atg10-expression-in-the-6-hydroxydopamine-rat-model-of-parkinson-s-disease
#19
Marzieh Shams Nooraei, Ali Noori-Zadeh, Shahram Darabi, Farzad Rajaei, Zohreh Golmohammadi, Hojjat Allah Abbaszadeh
Background: Autophagy is a mechanism disassembling the damaged organelles from the cell. This study attempted to examine the expression of several autophagy-related genes in Parkinson’s disease (PD) rat model. Methods: The male Wistar rats were divided into three groups as control, sham, and lesion. In the latter group, the PD rat model was induced by the injection of 6-hydroxydopamine in the striatum.The behavioral test was conducted one (baseline) and four weeks after the surgery through apomorphine hydrochloride...
July 23, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28732075/comprehensive-selection-of-reference-genes-for-quantitative-rt-pcr-analysis-of-murine-extramedullary-hematopoiesis-during-development
#20
Giuliana Medrano, Peihong Guan, Amanda J Barlow-Anacker, Ankush Gosain
The purpose of this study was to perform a comprehensive evaluation and selection of reference genes for the study of extramedullary hematopoiesis during development and the early post-natal period. A total of six candidate reference genes (ACTB, GAPDH, HPRT1, PPID, TBP, TUBB3) in four organs (heart, liver, spleen, and thymus) over five perinatal time points (Embryonic days 14.5, 16.5, 18.5, Post-natal days 0, 21) were evaluated by quantitative real-time PCR. The expression stability of the candidate reference genes were analyzed using geNorm, NormFinder, Bestkeeper, Delta CT method, and RefFinder software packages...
2017: PloS One
keyword
keyword
67345
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"