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https://www.readbyqxmd.com/read/29777694/gapdh-targeted-therapy-a-new-approach-for-secondary-damage-after-traumatic-brain-injury-on-rats
#1
Vladimir F Lazarev, Elizaveta A Dutysheva, Elena Y Komarova, Elena R Mikhaylova, Irina V Guzhova, Boris A Margulis
Massive neuronal death caused by a neurodegenerative pathology or damage due to ischaemia or traumatic brain injury leads to the appearance of cytosolic proteins in the extracellular space. We found that one of the most abundant cellular polypeptides, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), appearing in the medium of dying cells or body fluids is able to form aggregates that are cytotoxic to adjacent cells. Since we previously showed that the hydrocortisone derivative RX624 can inhibit the ability of GAPDH to transport the enzyme complex with polyglutamine and reduce the cytotoxicity of the complex, we explored the effects of GAPDH on SH-SY5Y neuroblastoma cells...
May 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29762492/zika-virus-induces-autophagy-in-human-umbilical-vein-endothelial-cells
#2
Haoran Peng, Bin Liu, Toure Doueu Yves, Yanhua He, Shijie Wang, Hailin Tang, Hao Ren, Ping Zhao, Zhongtian Qi, Zhaoling Qin
Autophagy is a common strategy for cell protection; however, some viruses can in turn adopt cellular autophagy to promote viral replication. Zika virus (ZIKV) is the pathogen that causes Zika viral disease, and it is a mosquito-borne virus. However, its pathogenesis, especially the interaction between ZIKV and target cells during the early stages of infection, is still unclear. In this study, we demonstrate that infecting human umbilical vein endothelial cells (HUVEC) with ZIKV triggers cellular autophagy. We observed both an increase in the conversion of LC3-I to LC3-II and increased accumulation of fluorescent cells with LC3 dots, which are considered to be the two key indicators of autophagy...
May 15, 2018: Viruses
https://www.readbyqxmd.com/read/29753109/expression-of-glyceraldehyde-3-phosphate-dehydrogenase-on-the-surface-of-clostridium-perfringens-cells
#3
Nozomu Matsunaga, Haruka Shimizu, Kanako Fujimoto, Kanako Watanabe, Tsutomu Yamasaki, Naoya Hatano, Eiji Tamai, Seiichi Katayama, Yasuo Hitsumoto
During research to identify fibronectin (Fn)-binding proteins (Fbps) on the surface of Clostridium perfringens cells, we identified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a candidate Fbp. GAPDH is a glycolytic enzyme found in a wide range of prokaryotes and eukaryotes. The Fn-binding activity of recombinant C. perfringens GAPDH (rGAPDH) was investigated using both ligand blotting analysis and enzyme-linked immunosorbent assay (ELISA). rGAPDH strongly bound plasminogen but not laminin or gelatin...
May 9, 2018: Anaerobe
https://www.readbyqxmd.com/read/29738567/evaluation-and-selection-of-internal-reference-genes-from-two-and-six-row-u-s-malting-barley-varieties-throughout-micromalting-for-use-in-rt-qpcr
#4
Jason G Walling, Leslie A Zalapa, Marcus A Vinje
Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a popular method for measuring transcript abundance. The most commonly used method of interpretation is relative quantification and thus necessitates the use of normalization controls (i.e. reference genes) to standardize transcript abundance. The most popular gene targets for RT-qPCR are housekeeping genes because they are thought to maintain a static transcript level among a variety of samples. However, more recent studies have shown, several housekeeping genes are not reliably stable...
2018: PloS One
https://www.readbyqxmd.com/read/29729225/diagnostic-utility-of-glycosyltransferase-mrna-expression-in-gastric-cancer
#5
Showkat Ahmad Bhat, Manzoor Ur Rahman Mir, Sabhiya Majid, Tehseen Hassan, Muneeb U Rehman, Sonallah Kuchy
OBJECTIVE/BACKGROUND: Posttranslational modification of proteins, including glycosylation, is known to differ between normal and tumor cells. Altered glycosyltransferase levels have been observed in tumor tissues and their role in tumor metastasis and invasion has been implicated. In this study the role of altered glycosyltransferase messenger RNA (mRNA) levels in serum of gastric cancer patients as early markers of gastric cancer was evaluated. METHODS: In this case control study the expression profile of ppGalNAc-T6, GlcNAcT-V, ST3Gal I, ST3 Gal IV, and ST6GalNAc-I in normal healthy control and gastric cancer patients was compared...
April 27, 2018: Hematology/oncology and Stem Cell Therapy
https://www.readbyqxmd.com/read/29727516/interaction-between-adenylate-kinase-3-and-glyceraldehyde-3-phosphate-dehydrogenase-from-chlamydomonas-reinhardtii
#6
Yizhi Zhang, Hélène Launay, Fan Liu, Régine Lebrun, Brigitte Gontero
The critical and ubiquitous enzyme adenylate kinase (ADK) catalyzes the nucleotide phosphoryl exchange reaction: 2ADP ↔ ATP + AMP. The ADK3 in the chloroplasts of the green alga Chlamydomonas reinhardtii, bears an unusual C-terminal extension that is similar to the C-terminal end of the intrinsically disordered protein CP12. In this study, we report that this enzyme, when oxidized but not when reduced, is able to interact with the chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) forming a stable complex as shown by native electrophoresis and mass spectrometry...
May 4, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29727459/selection-and-evaluation-of-housekeeping-genes-as-endogenous-controls-for-quantification-of-mrna-transcripts-in-theileria-parva-using-quantitative-real-time-polymerase-chain-reaction-qpcr
#7
Teboho N Tsotetsi, Nicola E Collins, Marinda C Oosthuizen, Kgomotso P Sibeko-Matjila
The reliability of any quantitative real-time polymerase chain reaction (qPCR) experiment can be seriously compromised by variations between samples as well as between PCR runs. This usually result from errors in sample quantification, especially with samples that are obtained from different individuals and tissues and have been collected at various time intervals. Errors also arise from differences in qPCR efficiency between assays performed simultaneously to target multiple genes on the same plate. Consequently, the derived quantitative data for the target genes become distorted...
2018: PloS One
https://www.readbyqxmd.com/read/29725946/spectrophotometric-photodynamic-diagnosis-of-prostate-cancer-cells-excreted-in-voided-urine-using-5-aminolevulinic-acid
#8
Yasushi Nakai, Makito Miyake, Satoshi Anai, Shunta Hori, Yoshihiro Tatsumi, Yosuke Morizawa, Sayuri Onisi, Nobumichi Tanaka, Kiyohide Fujimoto
To evaluate the feasibility of photodynamic diagnosis using 5-aminolevulinic acid (PDD-ALA) for detection of prostate cancer (PCa) cells in urine samples after prostate massage in patients who were suspected to have PCa. One hundred and eighty-nine patients with abnormal digital rectal examination and/or an elevated prostate-specific antigen (PSA) level who underwent initial prostate biopsy were recruited. After prostate massage, the first 60 mL of voided urine was collected. For PDD-ALA, 50 mL was used. The rest of collected urine was used for polymerase chain reaction (PCR) of PSA and glyceraldehyde 3-phosphate dehydrogenase (GAPDH)...
May 4, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/29723493/validation-of-housekeeping-genes-for-quantitative-mrna-expression-analysis-in-oshv-1-infected-ark-clam-scapharca-broughtonii
#9
Lusheng Xin, Bowen Huang, Changming Bai, Chongming Wang
Ostreid herpesvirus-1 (OsHV-1) presents interspecies transmission among bivalves. Recently, events of mass mortalities of ark clams (Scapharca broughtonii) infected with OsHV-1 have been recorded. To accurately assess the gene responding patterns of ark clams post OsHV-1 infection, constant stable housekeeping genes (HKGs) are needed as internal control to normalize raw mRNA expression data. In this study, ten candidate HKGs were selected, including 18S rRNA (18S), beta-actin (ACT), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), NADH dehydrogenase subunit (NADH), Elongation factor-1a (EF-1a), Elongation factor-1β (EF-1β), Elongation factor-1γ (EF-1γ), Ribosomal protein L7 (RL7), Ribosomal protein L15 (RL15) and Ribosomal protein S18 (S18)...
April 30, 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29717994/a-cryoprotectant-induces-conformational-change-in-glyceraldehyde-3-phosphate-dehydrogenase
#10
Yong Ju Kim
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme, catalyses the conversion of D-glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate. While mammalian and yeast GAPDHs are multifunctional proteins that have additional functions beyond those involved in glycolysis, including reactions related to nuclear RNA transport, DNA replication/repair, membrane fusion and cellular apoptosis, Escherichia coli GAPDH (ecGAPDH) has only been reported to function in glycolysis. The S-loop of GAPDH is required for interaction with its cofactor and with other proteins...
May 1, 2018: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/29717010/signature-arsenic-detoxification-pathways-in-halomonas-sp-strain-gfaj-1
#11
Shuangju Wu, Lianrong Wang, Rui Gan, Tong Tong, Hao Bian, Zhiqiang Li, Shiming Du, Zixin Deng, Shi Chen
Since the original report that Halomonas sp. strain GFAJ-1 was capable of using arsenic instead of phosphorus to sustain growth, additional studies have been conducted, and GFAJ-1 is now considered a highly arsenic-resistant but phosphorus-dependent bacterium. However, the mechanisms supporting the extreme arsenic resistance of the GFAJ-1 strain remain unknown. In this study, we show that GFAJ-1 has multiple distinct arsenic resistance mechanisms. It lacks the genes to reduce arsenate, which is the essential step in the well-characterized resistance mechanism of arsenate reduction coupled to arsenite extrusion...
May 1, 2018: MBio
https://www.readbyqxmd.com/read/29713285/inhibition-of-streptococcus-suis-adhesion-and-biofilm-formation-in-vitro-by-water-extracts-of-rhizoma-coptidis
#12
Yan-Hua Li, Yong-Hui Zhou, Yong-Zhi Ren, Chang-Geng Xu, Xin Liu, Bing Liu, Jian-Qing Chen, Wen-Ya Ding, Yu-Lin Zhao, Yan-Bei Yang, Shuai Wang, Di Liu
Streptococcus suis is difficult to treat and responsible for various infections in humans and pigs. It can also form biofilms and induce persistent infections. Rhizoma Coptidis is a medicinal plant widely used in Traditional Chinese Medicine. Although the inhibitory effects of Rhizoma Coptidis on biofilm formation have been investigated in several studies, the ability of Rhizoma Coptidis to inhibit S. suis biofilm formation and the underlying mechanisms have not yet been reported. In this study, we showed that sub-minimal inhibitory concentrations (25 and 50 μg mL-1 ) of water extracts of Rhizoma Coptidis ( Coptis deltoidea C...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29707711/tightly-bound-polyelectrolytes-enhance-enzyme-proteolysis-and-destroy-amyloid-aggregates
#13
Diana B Evstafyeva, Vladimir A Izumrudov, Vladimir I Muronetz, Pavel I Semenyuk
The use of polyelectrolytes is a prospective approach to form nanocomplexes to transport different compounds including proteins. In many cases, the bound protein should be digested after delivery to the target. In the present work, we studied proteolysis of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the complexes with polyelectrolytes. We have found polyanions to enhance the proteolytic degradation of GAPDH by proteinase K and thermolysin. This effect seems to be caused by destabilization of the protein structure...
April 30, 2018: Soft Matter
https://www.readbyqxmd.com/read/29705001/dendritic-peptide-bolaamphiphiles-for-sirna-delivery-to-primary-adipocytes
#14
Alexander C Eldredge, Mark E Johnson, Yang Cao, Lin Zhang, Can Zhao, Zhengxia Liu, Qin Yang, Zhibin Guan
Obesity is a major risk factor for diabetes, heart disease and other health problems. Adipose tissue plays a central role in the development of obesity and obesity-associated diseases. Gene therapy targeting adipose tissue may provide a promising strategy for obesity treatment. However, nucleic acid delivery to adipose tissue or even cultured adipocytes is challenging due to low delivery efficacy and high toxicity of the current cationic lipid based delivery systems, or monoamphiphiles. Herein, we report using dendritic peptide bolaamphiphiles (bolas) to deliver siRNA to primary adipocytes and hepatocytes...
April 16, 2018: Biomaterials
https://www.readbyqxmd.com/read/29701710/an-integrated-approach-of-proteomics-and-computational-genetic-modification-effectiveness-analysis-to-uncover-the-mechanisms-of-flood-tolerance-in-soybeans
#15
Xin Wang, Katsumi Sakata, Setsuko Komatsu
Flooding negatively affects the growth of soybeans. Recently, omic approaches have been used to study abiotic stress responses in plants. To explore flood-tolerant genes in soybeans, an integrated approach of proteomics and computational genetic modification effectiveness analysis was applied to the soybean ( Glycine max L. (Merrill)). Flood-tolerant mutant and abscisic acid (ABA)-treated soybean plants were used as the flood-tolerant materials. Among the primary metabolism, glycolysis, fermentation, and tricarboxylic acid cycle were markedly affected under flooding...
April 26, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29701683/the-incorporation-of-strontium-to-improve-bone-regeneration-ability-of-mesoporous-bioactive-glasses
#16
Sonia Fiorilli, Giulia Molino, Carlotta Pontremoli, Giorgio Iviglia, Elisa Torre, Clara Cassinelli, Marco Morra, Chiara Vitale-Brovarone
Over the recent years, mesoporous bioactive glasses (MBGs) gained interest as bone regeneration systems, due to their excellent bioactivity and ability to release therapeutic molecules. In order to improve the bone regeneration ability of MBGs, the incorporation of Sr2+ ions, due to its recognized pro-osteogenenic potential, represents a very promising strategy. In this study, MBGs based on the SiO₂⁻CaO system and containing different percentages (2 and 4 mol %) of strontium were prepared by two synthesis methods, in the form of microspheres and nanoparticles...
April 26, 2018: Materials
https://www.readbyqxmd.com/read/29701140/expression-of-plasma-hsa-mir122-in-hbv-related-hepatocellular-carcinoma-hcc-in-vietnamese-patients
#17
Nguyen Bao Quoc, Nguyen Doan Nguyen Phuong, Tang Kim Ngan, Nguyen Thi Minh Linh, Pham Hung Cuong, Nguyen Ngoc Bao Chau
BACKGROUND: Hepatocellular carcinoma (HCC) is the leading cause of cancer-related death in the world and considered as one of the most susceptible cancers in humans. The microRNA molecule, hsa-miR122, considered as a potential biological marker linked with the injury of hepatocellular tissue, is the most common microRNA in human liver cancer. Understanding the expression profile of hsa-miR122 plays an important role in the diagnosis of HCC Objective: Identification and comparison of cut-off values of plasma hsa-miR122 expression were conducted in blood samples of healthy control, HBV infected and HBV-related HCC Vietnamese patients Method and result: Fifty-two blood samples of healthy control and HBV-related HCC cases, collected between 2015 and 2017 were obtained from Ho Chi Minh City Oncology Hospital, Vietnam...
April 27, 2018: MicroRNA
https://www.readbyqxmd.com/read/29700768/proteomic-analysis-of-cerebral-cortex-extracts-from-sus-scrofa-with-induced-hemorrhagic-stroke
#18
Alexander A Sidyakin, Anna L Kaysheva, Artur T Kopylov, Alexander V Lobanov, Sergei G Morozov
Cerebrovascular diseases, including stroke and micro stroke, are the main causes of death in contemporary society. Hemorrhagic stroke is the fast emerging defficiency in the brain function resulting from disturbance of blood supply to the brain caused by the rapture of blood vessels (Lopez et al. in Proteomics Clin Appl 6:190-200, 2012). The influence of a model hemorrhagic stroke on white pigs with the change in the protein profile of their cortical samples 24 h and 2 months after the stroke was examined using mass-spectrometric analysis...
April 26, 2018: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/29699713/the-glyceraldehyde-3-phosphate-dehydrogenase-of-the-shrimp-litopenaeus-vannamei-molecular-cloning-characterization-and-expression-during-hypoxia
#19
Laura Camacho-Jiménez, Alma B Peregrino-Uriarte, José A Martínez-Quintana, Gloria Yepiz-Plascencia
Some marine crustaceans like the white shrimp Litopenaeus vannamei are tolerant to environmental hypoxia. Under oxygen deprivation, shrimp tissues obtain energy by enhancing anaerobic glycolysis. In mammals, hypoxia increases the expression of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which has been shown a "moonlighting" role in cells. However, the effect of hypoxia on the GAPDH expression has not been studied in crustaceans. In the present work, we obtained a 2744 bp gene sequence with a 999 bp ORF split by a single intron...
April 17, 2018: Marine Environmental Research
https://www.readbyqxmd.com/read/29692553/validation-of-reference-genes-for-accurate-normalization-of-gene-expression-with-quantitative-real-time-pcr-in-haloxylon-ammodendron-under-different-abiotic-stresses
#20
Bo Wang, Huihui Du, Zhengpei Yao, Cai Ren, Li Ma, Jiao Wang, Hua Zhang, Hao Ma
Haloxylon ammodendron plays an important role in maintaining the structure and function of the entire ecosystem where it grows. No suitable reference genes have been reported in H. ammodendron plants to date. In this study, a total of 8 reference genes ( 18S , ACT1 , ACT7 , UBC18 , TUA5 , GAPDH , EF - 1α and UBQ10 ) were selected from the available trancriptome database, and the expression stability of these 8 candidate genes was validated under different abiotic stress with three different statistical algorithms (geNorm, NormFinder and BestKeeper)...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
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