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https://www.readbyqxmd.com/read/28339937/uterine-artery-leptin-receptors-during-the-ovarian-cycle-and-pregnancy-regulate-angiogenesis-in-ovine-uterine-artery-endothelial-cells
#1
Vladimir E Vargas, Rosalina Villalon Landeros, Gladys E Lopez, Jing Zheng, Ronald R Magness
Leptin regulates body weight, reproductive functions, blood pressure, endothelial function, and fetoplacental angiogenesis. Compared to the luteal phase, the follicular phase and pregnancy are physiological states of elevated estrogen, angiogenesis, and uterine blood flow (UBF). Little is known concerning leptin and its receptors (OB-R) for regulating uterine artery (UA) angiogenesis. We : 1) Ex vivo expression of OB-R in UA endothelium (UAendo) and UA vascular smooth muscle (UAvsm) is elevated in pregnant vs...
March 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28339479/mouse-newborn-cells-allow-highly-productive-mouse-cytomegalovirus-replication-constituting-a-novel-convenient-primary-cell-culture-system
#2
Vu Thuy Khanh Le-Trilling, Mirko Trilling
Mammalian cell culture is indispensable for most aspects of current biomedical research. Immortalized cell lines are very convenient, but transforming principles (e.g. oncogenic viruses or their oncogenes) can heavily influence the experimental outcome. Primary cells do not share this apparent disadvantage but are more laborious to generate. Certain viruses (e.g. mouse cytomegalovirus) do not replicate efficiently in most transformed cell lines. In the past, such viruses have been routinely propagated on primary mouse embryonic fibroblasts (MEF) established around day 17 (d17) of gestation...
2017: PloS One
https://www.readbyqxmd.com/read/28327173/a-new-paradigm-for-aedes-spp-surveillance-using-gravid-ovipositing-sticky-trap-and-ns1-antigen-test-kit
#3
Sai Ming Lau, Tock H Chua, Wan-Yussof Sulaiman, Sylvia Joanne, Yvonne Ai-Lian Lim, Shamala Devi Sekaran, Karuthan Chinna, Balan Venugopalan, Indra Vythilingam
BACKGROUND: Dengue remains a serious public health problem in Southeast Asia and has increased 37-fold in Malaysia compared to decades ago. New strategies are urgently needed for early detection and control of dengue epidemics. METHODS: We conducted a two year study in a high human density dengue-endemic urban area in Selangor, where Gravid Ovipositing Sticky (GOS) traps were set up to capture adult Aedes spp. mosquitoes. All Aedes mosquitoes were tested using the NS1 dengue antigen test kit...
March 21, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28321338/frequency-risk-factors-and-adverse-fetomaternal-outcomes-of-placenta-previa-in-northern-tanzania
#4
Elizabeth Eliet Senkoro, Amasha H Mwanamsangu, Fransisca Seraphin Chuwa, Sia Emmanuel Msuya, Oresta Peter Mnali, Benjamin G Brown, Michael Johnson Mahande
Background and Objective. Placenta previa (PP) is a potential risk factor for obstetric hemorrhage, which is a major cause of fetomaternal morbidity and mortality in developing countries. This study aimed to determine frequency, risk factors, and adverse fetomaternal outcomes of placenta previa in Northern Tanzania. Methodology. A retrospective cohort study was conducted using maternally-linked data from Kilimanjaro Christian Medical Centre birth registry spanning 2000 to 2015. All women who gave birth to singleton infants were studied...
2017: Journal of Pregnancy
https://www.readbyqxmd.com/read/28306228/diagnosis-natural-history-and-management-in-vascular-ehlers-danlos-syndrome
#5
Peter H Byers, John Belmont, James Black, Julie De Backer, Michael Frank, Xavier Jeunemaitre, Diana Johnson, Melanie Pepin, Leema Robert, Lynn Sanders, Nigel Wheeldon
Vascular Ehlers Danlos syndrome (vEDS) is an uncommon genetic disorders characterized by arterial aneurysm, dissection and rupture, bowel rupture, and rupture of the gravid uterus. The frequency is estimated as 1/50,000-1/200,000 and results from pathogenic variants in COL3A1, which encodes the chains of type III procollagen, a major protein in vessel walls and hollow organs. Initial diagnosis depends on the recognitions of clinical features, including family history. Management is complex and requires multiple specialists who can respond to and manage the major complications...
March 2017: American Journal of Medical Genetics. Part C, Seminars in Medical Genetics
https://www.readbyqxmd.com/read/28282765/the-effect-of-gestational-diabetes-pre-gravid-maternal-obesity-and-their-combination-diabesity-on-outcomes-of-singleton-gestations
#6
Isaac Blickstein, Reut Doyev, Andreja Trojner Bregar, Gabrijela Bržan Šimenc, Ivan Verdenik, Natasa Tul
OBJECTIVE: To assess the effect of the combination of gestational diabetes mellitus (GDM) and pre-gravid obesity ('diabesity') in singleton gestations. METHODS: We compared perinatal outcomes of singleton gestations in mothers with GDM and pre-gravid obesity, with GDM but with normal pre-gravid BMI, and obese mothers without GDM. RESULTS: We compared diabesity mothers (n = 1525, 24.4% of mothers with GDM, 9.9% of all obese women) to mothers with GDM but with normal BMI (n = 4704, 75...
March 1, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28274281/the-philometrid-nematode-philometroides-acreanensis-n-sp-from-the-stomach-wall-of-the-catfish-pimelodus-blochii-in-north-western-brazil
#7
P H O Cavalcante, F Moravec, C P Santos
A new nematode species, Philometroides acreanensis n. sp. (Philometridae), is described from female specimens recovered from the stomach wall of the freshwater catfish Pimelodus blochii Valenciennes (Pimelodidae) collected in the Acre River (Amazon River basin), Acre State, Brazil. Based on examination by light and scanning electron microscopy, the new species differs from the two other South American congeneric species mainly in the body length of the gravid female (240-280 mm), the length of the oesophagus (1...
March 9, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/28266058/chemicals-isolated-from-justicia-adhatoda-linn-reduce-fitness-of-the-mosquito-aedes-aegypti-l
#8
Annamalai Thanigaivel, Sengottayan Senthil-Nathan, Prabhakaran Vasantha-Srinivasan, Edward-Sam Edwin, Athirstam Ponsankar, Selvaraj Selin-Rani, Venkatraman Pradeepa, Muthiah Chellappandian, Kandaswamy Kalaivani, Ahmed Abdel-Megeed, Raman Narayanan, Kadarkarai Murugan
Extracts from Justicia adhatoda L. (Acanthaceae) strongly reduced the fitness of the mosquito, Aedes aegypti Linn. The methanolic extracts inhibited several enzymes responsible for protecting insects from oxidative and other damage, including glutathione-S-transferase, superoxide dismutase, cytochrome P450, and α- and β-esterases. They increased repellency (maximum repellency at 100 ppm) in host-seeking adult females using the "arm-in cage assay." Histopathological examination showed the extracts led to serious midgut cell damage...
March 7, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28261223/regulatory-b-cells-in-pregnancy-lessons-from-autoimmunity-graft-tolerance-and-cancer
#9
REVIEW
Ruth Marian Guzman-Genuino, Kerrilyn R Diener
The success of pregnancy is contingent on the maternal immune system recognizing and accommodating a growing semi-allogeneic fetus. Specialized subsets of lymphocytes capable of negative regulation are fundamental in this process, and include the regulatory T cells (Tregs) and potentially, regulatory B cells (Bregs). Most of our current understanding of the immune regulatory role of Bregs comes from studies in the fields of autoimmunity, transplantation tolerance, and cancer biology. Bregs control autoimmune diseases and can elicit graft tolerance by inhibiting the differentiation of effector T cells and dendritic cells (DCs), and activating Tregs...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28258352/blood-feeding-status-gonotrophic-cycle-and-survivorship-of-aedes-stegomyia-aegypti-l-diptera-culicidae-caught-in-churches-from-merida-yucatan-mexico
#10
C M Baak-Baak, A Ulloa-Garcia, N Cigarroa-Toledo, J C Tzuc Dzul, C Machain-Williams, O M Torres-Chable, J C Navarro, J E Garcia-Rejon
Blood-feeding status, gonotrophic cycle, and survival rates of Aedes (Stegmyia) aegypti (L.) was investigated in catholic churches from Merida, Yucatan. Female Ae. aegypti were caught using backpack aspirator during 25 consecutive days in rainy (2015) and dry season (2016). Blood-feeding status was determined by external examination of the abdomen and classified as unfed, fed, and gravid. Daily changes in the parous-nulliparous ratio were recorded, and the gonotrophic cycle length was estimated by a time series analysis...
March 3, 2017: Neotropical Entomology
https://www.readbyqxmd.com/read/28249007/safety-evaluation-of-a-vaccine-effect-in-maternal-reproductive-outcome-and-fetal-anomaly-frequency-in-rats-using-a-leishmanial-vaccine-as-a-model
#11
Rafaianne Q Moraes-Souza, Ana Paula Reinaque, Thaigra S Soares, Ana Luiza T Silva, Rodolfo C Giunchetti, Maria A S Takano, Milena A Akamatsu, Flávia S Kubrusly, Fernanda Lúcio-Macarini, Isaias Raw, Dmitri Iourtov, Paulo Lee Ho, Lilian L Bueno, Ricardo T Fujiwara, Gustavo T Volpato
While the immunogenic potential of the vaccination against infectious diseases was extensively shown, data on the safety assessment of recombinant proteins in vaccine formulations administered during pregnancy are still scarce. In the current study, the antigenicity of a vaccine against leishmaniasis (based on Leishmania braziliensis recombinant protein peroxidoxin) during pregnancy and possible maternal reproductive outcomes and fetal anomalies after immunization with a leishmanial vaccine or adjuvant alone (Bordetella pertussis derived MPLA adjuvant) were assessed...
2017: PloS One
https://www.readbyqxmd.com/read/28245259/estimated-impact-on-birth-weight-of-scaling-up-intermittent-preventive-treatment-of-malaria-in-pregnancy-given-sulphadoxine-pyrimethamine-resistance-in-africa-a-mathematical-model
#12
Patrick G T Walker, Jessica Floyd, Feiko Ter Kuile, Matt Cairns
BACKGROUND: Malaria transmission has declined substantially in the 21st century, but pregnant women in areas of sustained transmission still require protection to prevent the adverse pregnancy and birth outcomes associated with malaria in pregnancy (MiP). A recent call to action has been issued to address the continuing low coverage of intermittent preventive treatment of malaria in pregnancy (IPTp). This call has, however, been questioned by some, in part due to concerns about resistance to sulphadoxine-pyrimethamine (SP), the only drug currently recommended for IPTp...
February 2017: PLoS Medicine
https://www.readbyqxmd.com/read/28226044/care-for-women-involved-with-drugs-social-representations-of-nurses
#13
Andreia Silva Rodrigues, Jeane Freitas de Oliveira, Cleuma Sueli Santos Suto, Maria da Penha de Lima Coutinho, Mirian Santos Paiva, Simone Santos Souza
Objective: analyze social representations of nurses related to the care for women involved with drugs. Method: qualitative research founded on the theory of social representations, with 42 nurses from a public maternity ward of Salvador-BA, using identification questionnaire, test of free association of words, and semi-structured interview. We analyzed the structure of social representations and the similarity tree of free evocations, and also analyzed the content of interviews...
January 2017: Revista Brasileira de Enfermagem
https://www.readbyqxmd.com/read/28210634/potential-of-a-pharmacogenetic-guided-algorithm-to-predict-optimal-warfarin-dosing-in-a-high-risk-hispanic-patient-role-of-a-novel-nqo1-2-polymorphism
#14
Dagmar F Hernandez-Suarez, Karla Claudio-Campos, Javier E Mirabal-Arroyo, Bianca A Torres-Hernández, Angel López-Candales, Kyle Melin, Jorge Duconge
Deep abdominal vein thrombosis is extremely rare among thrombotic events secondary to the use of contraceptives. A case to illustrate the clinical utility of ethno-specific pharmacogenetic testing in warfarin management of a Hispanic patient is reported. A 37-year-old Hispanic Puerto Rican, non-gravid female with past medical history of abnormal uterine bleeding on hormonal contraceptive therapy was evaluated for abdominal pain. Physical exam was remarkable for unspecific diffuse abdominal tenderness, and general initial laboratory results-including coagulation parameters-were unremarkable...
October 2016: Journal of Investigative Medicine High Impact Case Reports
https://www.readbyqxmd.com/read/28196415/hierarchical-assembly-of-tough-bioelastomeric-egg-capsules-is-mediated-by-a-bundling-protein
#15
Jun Jie Loke, Akshita Kumar, Shawn Hoon, Chandra Verma, Ali Miserez
Marine snail egg capsules are shock-absorbing bioelastomers made from precursor "egg case proteins" (ECPs) that initially lack long-range order. During capsule formation, these proteins self-assemble into coiled-coil filaments that subsequently align into microscopic layers, a multiscale process which is crucial to the capsules' shock-absorbing properties. In this study, we show that the self-assembly of ECPs into their functional capsule material is mediated by a bundling protein that facilitates the aggregation of coiled-coil building blocks and their gelation into a prefinal capsule prior to final stabilization...
March 13, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28196149/climatic-and-geographic-predictors-of-life-history-variation-in-eastern-massasauga-sistrurus-catenatus-a-range-wide-synthesis
#16
Eric T Hileman, Richard B King, John M Adamski, Thomas G Anton, Robyn L Bailey, Sarah J Baker, Nickolas D Bieser, Thomas A Bell, Kristin M Bissell, Danielle R Bradke, Henry Campa, Gary S Casper, Karen Cedar, Matthew D Cross, Brett A DeGregorio, Michael J Dreslik, Lisa J Faust, Daniel S Harvey, Robert W Hay, Benjamin C Jellen, Brent D Johnson, Glenn Johnson, Brooke D Kiel, Bruce A Kingsbury, Matthew J Kowalski, Yu Man Lee, Andrew M Lentini, John C Marshall, David Mauger, Jennifer A Moore, Rori A Paloski, Christopher A Phillips, Paul D Pratt, Thomas Preney, Kent A Prior, Andrew Promaine, Michael Redmer, Howard K Reinert, Jeremy D Rouse, Kevin T Shoemaker, Scott Sutton, Terry J VanDeWalle, Patrick J Weatherhead, Doug Wynn, Anne Yagi
Elucidating how life history traits vary geographically is important to understanding variation in population dynamics. Because many aspects of ectotherm life history are climate-dependent, geographic variation in climate is expected to have a large impact on population dynamics through effects on annual survival, body size, growth rate, age at first reproduction, size-fecundity relationship, and reproductive frequency. The Eastern Massasauga (Sistrurus catenatus) is a small, imperiled North American rattlesnake with a distribution centered on the Great Lakes region, where lake effects strongly influence local conditions...
2017: PloS One
https://www.readbyqxmd.com/read/28190408/net-mineral-requirements-of-dairy-goats-during-pregnancy
#17
C J Härter, L D Lima, D S Castagnino, H O Silva, F O M Figueiredo, N R St-Pierre, K T Resende, I A M A Teixeira
Mineral requirements of pregnant dairy goats are still not well defined; therefore, we investigated the net Ca, P, Mg, Na and K requirements for pregnancy and for maintenance during pregnancy in two separate experiments. Experiment 1 was performed to estimate the net Ca, P, Mg, Na and K requirements in goats carrying single or twin fetuses from 50 to 140 days of pregnancy (DOP). The net mineral requirements for pregnancy were determined by measuring mineral deposition in gravid uterus and mammary gland after comparative slaughter...
February 13, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28182343/comparison-of-amniotic-fluid-cytokine-levels-in-postterm-and-term-pregnancy-a-prospective-study
#18
Vakkas Korkmaz, Yasemin Cekmez, Hatice Surer, Canan D Deveci, Fatma D Ocal, Murad Gezer, Zehra Kurdoglu, Yusuf Ergun, Tuncay Kucukozkan
BACKGROUND: To evaluate amniotic fluid pro- and anti-inflammatory cytokine levels in women with postterm and term pregnancies in labor and not in labor. METHODS: The study involved three groups: postterm (Group 1, n = 29), term in labor (Group 2, n = 28), and control (Group 3, n = 30). All groups were compared with respect to age, gravidity, parity, obstetric history, gestation week, cervical dilatation and effacement, maternal serum C-reactive protein and white cell count, amniotic interleukin 4, 6, and 10 levels, birthweight, and cord blood pH...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182103/filarial-abscess-aspiration-of-adult-gravid-female-worm-from-submandibular-region-an-unusual-presentation
#19
Ruquiya Afrose, Mohammad Feroz Alam, Syed Shamshad Ahmad, Mohammed Naim
Microfilaria is a major public health problem in tropical and subtropical countries and is an endemic problem in India. Wuchereria bancrofti is the most common filarial infection. In some cases, microfilariae and adult filarial worm have been incidentally detected in fine-needle aspirates of various lesions; detection of microfilaria from subcutaneous site or from abscess site is even rarer. We here report an unusual case of Bancroftian microfilariasis in a 68-year-old female coming from endemic area presenting with right submandibular abscess...
January 2017: Journal of Cytology
https://www.readbyqxmd.com/read/28178169/an-artificial-neural-network-prediction-model-of-congenital-heart-disease-based-on-risk-factors-a-hospital-based-case-control-study
#20
Huixia Li, Miyang Luo, Jianfei Zheng, Jiayou Luo, Rong Zeng, Na Feng, Qiyun Du, Junqun Fang
An artificial neural network (ANN) model was developed to predict the risks of congenital heart disease (CHD) in pregnant women.This hospital-based case-control study involved 119 CHD cases and 239 controls all recruited from birth defect surveillance hospitals in Hunan Province between July 2013 and June 2014. All subjects were interviewed face-to-face to fill in a questionnaire that covered 36 CHD-related variables. The 358 subjects were randomly divided into a training set and a testing set at the ratio of 85:15...
February 2017: Medicine (Baltimore)
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