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https://www.readbyqxmd.com/read/28222353/clogging-in-permeable-concrete-a-review
#1
REVIEW
Alalea Kia, Hong S Wong, Christopher R Cheeseman
Permeable concrete (or "pervious concrete" in North America) is used to reduce local flooding in urban areas and is an important sustainable urban drainage system. However, permeable concrete exhibits reduction in permeability due to clogging by particulates, which severely limits service life. This paper reviews the clogging mechanism and current mitigating strategies in order to inform future research needs. The pore structure of permeable concrete and characteristics of flowing particulates influence clogging, which occurs when particles build-up and block connected porosity...
February 18, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28221789/raman-spectroscopy-reveals-selective-interactions-of-cytochrome-c-with-cardiolipin-that-correlate-with-membrane-permeability
#2
Jay P Kitt, David A Bryce, Shelley D Minteer, Joel M Harris
Permeabilization of the outer mitochondrial membrane is an integral step in apoptosis. The resulting release of pro-apoptotic signaling proteins leads to cell destruction through activation of the cysteine-aspartic protease (caspase) cascade. However, the mechanism of outer mitochondrial membrane (OMM) permeabilization remains unclear. It was recently shown that cytochrome c can induce pore formation in cardiolipin-containing phospholipid membranes leading to large dextran and protein permeability. In this work, the interaction of cytochrome c with cardiolpin-containing phospholipid vesicles, serving as models of the OMM, is investigated to probe cytochrome c-induced permeability...
February 21, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28221766/proteoform-profile-mapping-of-the-human-serum-complement-component-c9-reveals-unexpected-new-features-of-n-o-and-c-glycosylation
#3
Vojtech Franc, Yang Yang, Albert J R Heck
The human complement C9 protein (~65 kDa) is a member of the complement pathway. It plays an essential role in the membrane attack complex (MAC), which forms a lethal pore on the cellular surface of pathogenic bacteria. Here, we charted in detail the structural micro-heterogeneity of C9 purified from human blood serum, using an integrative workflow combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics. The proteoform profile of C9 was acquired by high-resolution native mass spectrometry, which revealed the co-occurrence of ~50 distinct MS signals...
February 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28221134/human-nup98-regulates-the-localization-and-activity-of-dexh-d-box-helicase-dhx9
#4
Juliana S Capitanio, Ben Montpetit, Richard W Wozniak
Beyond their role at nuclear pore complexes, some nucleoporins function in the nucleoplasm. One such nucleoporin, Nup98, binds chromatin and regulates gene expression. To gain insight into how Nup98 contributes to this process, we focused on identifying novel binding partners and understanding the significance of these interactions. Here we report on the identification of the DExH/D-box helicase DHX9 as an intranuclear Nup98 binding partner. Various results, including in vitro assays, show that the FG/GLFG region of Nup98 binds to N- and C-terminal regions of DHX9 in an RNA facilitated manner...
February 21, 2017: ELife
https://www.readbyqxmd.com/read/28220789/stim1-activates-crac-channels-through-rotation-of-the-pore-helix-to-open-a-hydrophobic-gate
#5
Megumi Yamashita, Priscilla S-W Yeung, Christopher E Ing, Beth A McNally, Régis Pomès, Murali Prakriya
Store-operated Ca(2+) release-activated Ca(2+) (CRAC) channels constitute a major pathway for Ca(2+) influx and mediate many essential signalling functions in animal cells, yet how they open remains elusive. Here, we investigate the gating mechanism of the human CRAC channel Orai1 by its activator, stromal interacting molecule 1 (STIM1). We find that two rings of pore-lining residues, V102 and F99, work together to form a hydrophobic gate. Mutations of these residues to polar amino acids produce channels with leaky gates that conduct ions in the resting state...
February 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/28220076/actions-and-mechanisms-of-polyunsaturated-fatty-acids-on-voltage-gated-ion-channels
#6
REVIEW
Fredrik Elinder, Sara I Liin
Polyunsaturated fatty acids (PUFAs) act on most ion channels, thereby having significant physiological and pharmacological effects. In this review we summarize data from numerous PUFAs on voltage-gated ion channels containing one or several voltage-sensor domains, such as voltage-gated sodium (NaV), potassium (KV), calcium (CaV), and proton (HV) channels, as well as calcium-activated potassium (KCa), and transient receptor potential (TRP) channels. Some effects of fatty acids appear to be channel specific, whereas others seem to be more general...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28219688/mesoporous-phenylalanine-ammonia-lyase-microspheres-with-improved-stability-through-calcium-carbonate-templating
#7
Jiandong Cui, Yamin Zhao, Zhilei Tan, Cheng Zhong, Peipei Han, Shiru Jia
Cross-linked enzyme aggregates (CLEAs) have recently emerged as a promising method for enzyme immobilization due to its simplicity and low cost. However, a lack of good size and morphological control over the as-prepared CLEAs has limited their practical applications in some cases. Here, monodisperse spherical CLEAs of phenylalanine ammonia lyase (PAL microspheres) were prepared based on CaCO3 microtemplates. The preparation procedure involves filling porous CaCO3 microtemplates with the protein by salt precipitation, glutaraldehyde crosslinking, and dissolution of the microtemplates...
February 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28219343/computing-structure-based-lipid-accessibility-of-membrane-proteins-with-mp_lipid_acc-in-rosettamp
#8
Julia Koehler Leman, Sergey Lyskov, Richard Bonneau
BACKGROUND: Membrane proteins are underrepresented in structural databases, which has led to a lack of computational tools and the corresponding inappropriate use of tools designed for soluble proteins. For membrane proteins, lipid accessibility is an essential property. Although programs are available for sequence-based prediction of lipid accessibility and structure-based identification of solvent-accessible surface area, the latter does not distinguish between water accessible and lipid accessible residues in membrane proteins...
February 20, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28218828/a-simple-and-facile-approach-to-create-charge-reversible-pores-via-hydrophobic-anchoring-of-ionic-amphiphiles
#9
Sonu Kizhakeppura, B V V S Pavan Kumar, Subi J George, Muthusamy Eswaramoorthy
Mesoporous silica based charge reversal systems have gained significant attention in the recent years due to a variety of applications such as drug delivery, dye adsorption, catalysis, chromatography etc. Such systems often use covalent strategies to immobilize functional groups on the silica scaffold. However, lack of dynamism, modularity and post-synthetic flexibility associated with covalent routes limit their wider applicability. Alternatively, supramolecular routes are gaining increased attention owing to their ability to overcome these limitations...
February 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28218748/hiv-tat-protein-and-amyloid-%C3%AE-peptide-form-multifibrillar-structures-that-cause-neurotoxicity
#10
Alina Hategan, Mario A Bianchet, Joseph Steiner, Elena Karnaukhova, Eliezer Masliah, Adam Fields, Myoung-Hwa Lee, Alex M Dickens, Norman Haughey, Emilios K Dimitriadis, Avindra Nath
Deposition of amyloid-β plaques is increased in the brains of HIV-infected individuals, and the HIV transactivator of transcription (Tat) protein affects amyloidogenesis through several indirect mechanisms. Here, we investigated direct interactions between Tat and amyloid-β peptide. Our in vitro studies showed that in the presence of Tat, uniform amyloid fibrils become double twisted fibrils and further form populations of thick unstructured filaments and aggregates. Specifically, Tat binding to the exterior surfaces of the Aβ fibrils increases β-sheet formation and lateral aggregation into thick multifibrillar structures, thus producing fibers with increased rigidity and mechanical resistance...
February 20, 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/28218678/adsorptive-desulfurization-of-model-gasoline-by-using-different-zn-sources-exchanged-nay-zeolites
#11
Jingwei Rui, Fei Liu, Rijie Wang, Yanfei Lu, Xiaoxia Yang
A series of Zn-modified NaY zeolites were prepared by the liquid-phase ion-exchange method with different Zn sources, including Zn(NO₃)₂, Zn(Ac)₂ and ZnSO₄. The samples were tested as adsorbents for removing an organic sulfur compound from a model gasoline fuel containing 1000 ppmw sulfur. Zn(Ac)₂-Y exhibited the best performance for the desulfurization of gasoline at ambient conditions. Combined with the adsorbents' characterization results, the higher adsorption capacity of Zn(Ac)₂-Y is associated with a higher ion-exchange degree...
February 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28218528/a-water-stable-anionic-metal-organic-framework-constructed-from-columnar-zinc-adeninate-units-for-highly-selective-light-hydrocarbon-separation-and-efficient-separation-of-organic-dyes
#12
Hui-Fang Ma, Qing-Yan Liu, Yu-Ling Wang, Shun-Gao Yin
A metal-organic framework (MOF), {(Me2NH2)2[Zn6(μ4-O)(ad)4(BPDC)4]}n (JXNU-4; ad(-) = adeninate), with an anionic three-dimensional (3D) framework constructed from one-dimensional (1D) columnar [Zn6(ad)4(μ4-O)]n secondary building units (SBUs) and 4,4'-biphenyldicarboxylate (BPDC(2-)) ligand, was prepared. The anionic 3D framework has 1D square channels with an aperture of about 9.8 Å and exhibits a carboxylate-O-decorated pore environment. The microporous nature of JXNU-4 was established by the N2 adsorption data, which gives Langmuir and Brumauer-Emmett-Teller surface areas of 1800 and 1250 m(2) g(-1), respectively...
February 20, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28218479/systematic-study-of-functionalizable-non-biofouling-agarose-films-with-protein-and-cellular-patterns-on-glass-slides
#13
Gyeongyeop Han, Daehwa Hong, Bong Soo Lee, EunRae Ha, Ji Hun Park, Insung Choi, Sung Min Kang, JungKyu Lee
We demonstrated a systematic investigation on chemically functionalizable, non-biofouling agarose films over large-area glass surfaces. Agarose films, prepared with varying concentrations of agarose (aq), were activated by periodate oxidation to generate aldehyde groups at the termini of agarose chains. The non-biofouling efficacies and binding capabilities of the activated films were evaluated using protein and cellular patterning, assisted by a microarrayer, microcontact printing, and micromolding in capillaries...
February 20, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28218313/ultrasmall-fe2o3-nanoparticles-mos2-nanosheets-composite-as-high-performance-anode-material-for-lithium-ion-batteries
#14
Bin Qu, Yue Sun, Lianlian Liu, Chunyan Li, Changjian Yu, Xitian Zhang, Yujin Chen
Coupling ultrasmall Fe2O3 particles (~4.0 nm) with the MoS2 nanosheets is achieved by a facile method for high-performance anode material for Li-ion battery. MoS2 nanosheets in the composite can serve as scaffolds, efficiently buffering the large volume change of Fe2O3 during charge/discharge process, whereas the ultrasmall Fe2O3 nanoparticles mainly provide the specific capacity. Due to bigger surface area and larger pore volume as well as strong coupling between Fe2O3 particles and MoS2 nanosheets, the composite exhibits superior electrochemical properties to MoS2, Fe2O3 and the physical mixture Fe2O3+MoS2...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28218295/graphene-induced-pore-formation-on-cell-membranes
#15
Guangxin Duan, Yuanzhao Zhang, Binquan Luan, Jeffrey K Weber, Royce W Zhou, Zaixing Yang, Lin Zhao, Jiaying Xu, Judong Luo, Ruhong Zhou
Examining interactions between nanomaterials and cell membranes can expose underlying mechanisms of nanomaterial cytotoxicity and guide the design of safer nanomedical technologies. Recently, graphene has been shown to exhibit potential toxicity to cells; however, the molecular processes driving its lethal properties have yet to be fully characterized. We here demonstrate that graphene nanosheets (both pristine and oxidized) can produce holes (pores) in the membranes of A549 and Raw264.7 cells, substantially reducing cell viability...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28217845/probing-zeolite-crystal-architecture-and-structural-imperfections-using-differently-sized-fluorescent-organic-probe-molecules
#16
Bert Marc Weckhuysen, Frank Hendriks, Joel Schmidt, Jeroen Rombouts, Koen Lammertsma, Pieter Bruijnincx
A micro-spectroscopic method has been developed to probe the accessibility of zeolite crystals using a series of fluorescent 4-(4-diethylaminostyryl)-1-methylpyridinium iodide (DAMPI) probes of increasing molecular size. Staining of large zeolite crystals with MFI topology and subsequent mapping of the resulting fluorescence using confocal fluorescence microscopy reveal differences in structural integrity: the 90° intergrowth sections of MFI crystals are prone to develop structural imperfections, which act as entrance routes for the probes into the zeolite crystal...
February 19, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28217387/histological-assessment-of-porous-custom-made-hydroxyapatite-implants-6-months-and-2-5-years-after-cranioplasty
#17
Hajime Ono, Taigen Sase, Yuichiro Tanaka, Hiroshi Takasuna
BACKGROUND: In cranial reconstruction, the features of artificial bone differ. Custom-made porous hydroxyapatite (HAp) implants for cranioplasty have been used all over the world because of their good cosmetic, biocompatibility, and osteoconductive properties. Surgical techniques were analyzed, and histological assessment of new bone formation in the hydroxyapatite was performed. METHODS: Over a 6-year time period, 41 patients underwent cranioplasty using a custom-made three-dimensional hybrid pore structured hydroxyapatite (3DHPoHAp) implant...
2017: Surgical Neurology International
https://www.readbyqxmd.com/read/28217261/affinity-of-rimantadine-enantiomers-against-influenza-a-m2-protein-revisited
#18
Antonios Drakopoulos, Christina Tzitzoglaki, Chulong Ma, Kathrin Freudenberger, Anja Hoffmann, Yanmei Hu, Günter Gauglitz, Michaela Schmidtke, Jun Wang, Antonios Kolocouris
Recent findings from solid state NMR (ssNMR) studies suggested that the (R)-enantiomer of rimantadine binds to the full M2 protein with higher affinity than the (S)-enantiomer. Intrigued by these findings, we applied functional assays, such as antiviral assay and electrophysiology (EP), to evaluate the binding affinity of rimantadine enantiomers to the M2 protein channel. Unexpectedly, no significant difference was found between the two enantiomers. Our experimental data based on the full M2 protein function were further supported by alchemical free energy calculations and isothermal titration calorimetry (ITC) allowing an evaluation of the binding affinity of rimantadine enantiomers to the M2TM pore...
February 9, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28216048/chloroquine-blocks-the-kir4-1-channels-by-an-open-pore-blocking-mechanism
#19
Leticia G Marmolejo-Murillo, Iván A Aréchiga-Figueroa, Eloy G Moreno-Galindo, Ricardo A Navarro-Polanco, Aldo A Rodríguez-Menchaca, Meng Cui, José A Sánchez-Chapula, Tania Ferrer
Kir4.1 channels have been implicated in various physiological processes, mainly in the K(+) homeostasis of the central nervous system and in the control of glial function and neuronal excitability. Even though, pharmacological research of these channels is very limited. Chloroquine (CQ) is an amino quinolone derivative known to inhibit Kir2.1 and Kir6.2 channels with different action mechanism and binding site. Here, we employed patch-clamp methods, mutagenesis analysis, and molecular modeling to characterize the molecular pharmacology of Kir4...
February 16, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28215876/in-vitro-lectin-binding-to-the-outer-surface-of-spirocerca-lupi-at-different-life-stages
#20
I Aroch, I Arogeti, A Marcovics, Y Spiegel, E Lavy
Spirocerca lupi is the esophageal nematode of dogs. Early, transient eosinophilia occurs in experimentally infected dogs, but is absent in advanced cases, suggesting that the nematode evades the dog's immune system. Lectins are proteins or glycoproteins of plant or animal origin, binding different saccharides, with varying specificities and avidities, used to characterize surface haptens in plant and animal parasitic helminths. This study investigated the in vitro binding of six lectins (Concanavalin A [ConA], wheat germ agglutinin [WGA], peanut agglutinin [PNA], soybean agglutinin [SBA], Dolichus biflorus agglutinin [DBA] and Ulex earopaeus agglutinin I [UEA]) to the surface of S...
February 15, 2017: Veterinary Parasitology
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