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TRP channel

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https://www.readbyqxmd.com/read/29221175/trpm8-is-required-for-survival-and-radioresistance-of-glioblastoma-cells
#1
Dominik Klumpp, Stephanie C Frank, Lukas Klumpp, Efe C Sezgin, Marita Eckert, Lena Edalat, Martin Bastmeyer, Daniel Zips, Peter Ruth, Stephan M Huber
TRPM8 is a Ca2+-permeable nonselective cation channel belonging to the melastatin sub-group of the transient receptor potential (TRP) family. TRPM8 is aberrantly overexpressed in a variety of tumor entities including glioblastoma multiforme where it reportedly contributes to tumor invasion. The present study aimed to disclose further functions of TRPM8 in glioma biology in particular upon cell injury by ionizing radiation. To this end, TCGA data base was queried to expose the TRPM8 mRNA abundance in human glioblastoma specimens and immunoblotting was performed to analyze the TRPM8 protein abundance in primary cultures of human glioblastoma...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29217583/structure-of-the-cold-and-menthol-sensing-ion-channel-trpm8
#2
Ying Yin, Mengyu Wu, Lejla Zubcevic, William F Borschel, Gabriel C Lander, Seok-Yong Lee
Transient receptor potential melastatin (TRPM) cation channels are polymodal sensors that are involved in a variety of physiological processes. Within the TRPM family, member 8 (TRPM8) is the primary cold- and menthol-sensor in humans. We determined the cryo-electron microscopy structure of the full-length TRPM8 from the collared flycatcher at an overall resolution of ~4.1 Å. Our TRPM8 structure reveals a three-layered architecture. The amino-terminal domain with a fold distinct among known TRP structures, together with the carboxyl-terminal region, forms a large two-layered cytosolic ring that extensively interacts with the transmembrane channel layer...
December 7, 2017: Science
https://www.readbyqxmd.com/read/29217581/structure-of-the-human-trpm4-ion-channel-in-a-lipid-nanodisc
#3
Henriette E Autzen, Alexander G Myasnikov, Melody G Campbell, Daniel Asarnow, David Julius, Yifan Cheng
Transient receptor potential (TRP) melastatin 4 (TRPM4) is a widely expressed cation channel associated with a variety of cardiovascular disorders. TRPM4 is activated by increased intracellular calcium in a voltage dependent manner, but unlike many other TRP channels is permeable to monovalent cations only. Here we present two structures of full-length human TRPM4 embedded in lipid nanodiscs at ~3Å resolution as determined by single particle electron cryo-microscopy. These structures, with and without calcium bound, reveal a general architecture for this major subfamily of TRP channels and a well-defined calcium binding site within the intracellular side of the S1-S4 domain...
December 7, 2017: Science
https://www.readbyqxmd.com/read/29214666/low-level-ultrahigh-frequency-and-ultrashort-pulse-blue-laser-irradiation-enhances-osteoblast-extracellular-calcification-by-upregulating-proliferation-and-differentiation-via-transient-receptor-potential-vanilloid-1
#4
Risako Mikami, Koji Mizutani, Akira Aoki, Yukihiko Tamura, Kazuhiro Aoki, Yuichi Izumi
BACKGROUND AND OBJECTIVE: Low-level laser irradiation (LLLI) exerts various biostimulative effects, including promotion of wound healing and bone formation; however, few studies have examined biostimulation using blue lasers. The purpose of this study was to investigate the effects of low-level ultrahigh-frequency (UHF) and ultrashort-pulse (USP) blue laser irradiation on osteoblasts. STUDY DESIGN/ MATERIALS AND METHODS: The MC3T3-E1 osteoblast cell line was used in this study...
December 7, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/29211714/structures-of-the-calcium-activated-non-selective-cation-channel-trpm4
#5
Jiangtao Guo, Ji She, Weizhong Zeng, Qingfeng Chen, Youxing Jiang, Xiao-Chen Bai
TRPM4 is a calcium-activated, phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) -modulated, non-selective cation channel that belongs to the family of melastatin-related transient receptor potential (TRPM) channels. Here we present the electron cryo-microscopy structures of the mouse TRPM4 channel with and without ATP. TRPM4 consists of multiple transmembrane and cytosolic domains, which assemble into a three-tiered architecture. The N-terminal nucleotide-binding domain and the C-terminal coiled-coil participate in the tetrameric assembly of the channel; ATP binds at the nucleotide-binding domain and inhibits channel activity...
December 6, 2017: Nature
https://www.readbyqxmd.com/read/29210126/crosslink-of-calcium-and-sodium-signalling-in-health-and-disease
#6
Alexei Verkhratsky, Mohamed Trebak, Fabiana Perocchi, Daniel Khananishvili, Israel Sekler
Transmembrane ionic gradients, that are indispensable feature of life, are utilised for generation of cytosolic ionic signals that regulate a host of cellular functions. Intracellular signalling mediated by Ca2+ and Na+ is tightly linked through several molecular pathways that generate Ca2+ and Na+ fluxes and are in turn regulated by both ions. Transient receptor potential (TRP) channels bridge endoplasmic reticulum Ca2+ release with generation of Na+ and Ca2+ currents. Plasmalemmal Na+ /Ca2+ exchanger by flickering between forward and reverse mode coordinates influx and efflux of both ions with membrane polarisation and cytosolic ion concentration...
December 6, 2017: Experimental Physiology
https://www.readbyqxmd.com/read/29207921/allyl-isothiocyanate-increases-carbohydrate-oxidation-through-enhancing-insulin-secretion-by-trpv1
#7
Noriyuki Mori, Manami Kurata, Hanae Yamazaki, Shigenobu Matsumura, Takashi Hashimoto, Kazuki Kanazawa, Tomonori Nadamoto, Kazuo Inoue, Tohru Fushiki
The transient receptor potential (TRP) V1 is a cation channel belonging to the TRP channel family and it has been reported to be involved in energy metabolism, especially glucose metabolism. While, we have previously shown that intragastric administration of allyl isothiocyanate (AITC) enhanced glucose metabolism via TRPV1, the underlying mechanism has not been elucidated. In this study, we examined the relationship between insulin secretion and the increase in carbohydrate oxidation due to AITC. Intragastric administration of AITC elevated blood insulin levels in mice and AITC directly enhanced insulin secretion from isolated islets...
December 6, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29204735/inflammaging-in-cervical-and-lumbar-degenerated-intervertebral-discs-analysis-of-proinflammatory-cytokine-and-trp-channel-expression
#8
Aleksandra Sadowska, Ermioni Touli, Wolfgang Hitzl, Helen Greutert, Stephen J Ferguson, Karin Wuertz-Kozak, Oliver N Hausmann
PURPOSE: To investigate and compare the occurrence of inflammatory processes in the sites of disc degeneration in the lumbar and cervical spine by a gene array and subsequent qPCR and to investigate the mechanistic involvement of transient receptor potential channels TRPC6 and TRPV4. METHODS: The gene expression of inflammatory cytokines and TRP channels was measured in human disc samples obtained from patients undergoing discectomy at the cervical (n = 24) or lumbar (n = 27) spine for degenerative disc disease (DDD) and disc herniation (DH) and analyzed for differences with regard to spinal level, IVD degeneration grade, Modic grade, age, sex, disc region and surgical extent...
December 4, 2017: European Spine Journal
https://www.readbyqxmd.com/read/29198958/inhalation-anaesthetic-isoflurane-inhibits-the-muscarinic-cation-current-and-carbachol-induced-gastrointestinal-smooth-muscle-contractions
#9
Dariia Dryn, Jialie Luo, Mariia Melnyk, Alexander Zholos, Hongzhen Hu
Gastrointestinal tract motility may be demoted significantly after surgery operations at least in part due to anaesthetic agents, but there is no comprehensive explanation of the molecular mechanism(s) of such adverse effects. Anesthetics are known to interact with various receptors and ion channels including several subtypes of transient receptor potential (TRP) channels. Two members of the canonical subfamily of TRP channels (TRPC), TRPC4 and TRPC6 are Ca2+-permeable cation channels involved in visceral smooth muscle contractility induced by acetylcholine, the primary excitatory neurotransmitter in the gut...
November 30, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29187363/trpv4-heats-up-ano1-dependent-exocrine-gland-fluid-secretion
#10
Sandra Derouiche, Yasunori Takayama, Masataka Murakami, Makoto Tominaga
Several ion channels and transporters regulate fluid secretion in salivary and lacrimal glands. In salivary glands, the major anion channel involved in fluid secretion is the calcium-activated chloride channel anoctamin 1 (ANO1). Several members of the transient receptor potential (TRP) channel superfamily regulate ANO1 activity. Here, we report a functional interaction between thermosensitive TRP vanilloid (TRPV)4 and ANO1 in acinar cells isolated from mouse salivary and lacrimal glands. TRPV4 activation induced chloride currents and shrinkage of acinar cells by increasing intracellular calcium concentrations...
November 29, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29175331/involvement-of-trpv3-and-trpm8-ion-channel-proteins-in-induction-of-mammalian-cold-inducible-proteins
#11
Takanori Fujita, Yu Liu, Hiroaki Higashitsuji, Katsuhiko Itoh, Koji Shibasaki, Jun Fujita, Hiroyuki Nishiyama
Cold-inducible RNA-binding protein (CIRP), RNA-binding motif protein 3 (RBM3) and serine and arginine rich splicing factor 5 (SRSF5) are RNA-binding proteins that are transcriptionally upregulated in response to moderately low temperatures and a variety of cellular stresses in mammalian cells. Induction of these cold-inducible proteins (CIPs) is dependent on transient receptor potential (TRP) V4 channel protein, but seems independent of its ion channel activity. We herein report that in addition to TRPV4, TRPV3 and TRPM8 are necessary for the induction of CIPs...
November 21, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29164310/relevance-of-trpa1-and-trpm8-channels-as-vascular-sensors-of-cold-in-the-cutaneous-microvasculature
#12
REVIEW
Y Pan, D Thapa, L Baldissera, F Argunhan, A A Aubdool, S D Brain
Cold exposure is directly related to skin conditions, such as frostbite. This is due to the cold exposure inducing a vasoconstriction to reduce cutaneous blood flow and protect against heat loss. However, a long-term constriction will cause ischaemia and potentially irreversible damage. We have developed techniques to elucidate the mechanisms of the vascular cold response. We focused on two ligand-gated transient receptor potential (TRP) channels, namely, the established "cold sensors" TRP ankyrin 1 (TRPA1) and TRP melastin (TRPM8)...
November 21, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/29163050/mechanisms-of-cisplatin-induced-ototoxicity-and-otoprotection
#13
REVIEW
Sandeep Sheth, Debashree Mukherjea, Leonard P Rybak, Vickram Ramkumar
Evidence of significant hearing loss during the early days of use of cisplatin as a chemotherapeutic agent in cancer patients has stimulated research into the causes and treatment of this side effect. It has generally been accepted that hearing loss is produced by excessive generation of reactive oxygen species (ROS) in cell of the cochlea, which led to the development of various antioxidants as otoprotective agents. Later studies show that ROS could stimulate cochlear inflammation, suggesting the use of anti-inflammatory agents for treatment of hearing loss...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29159026/highlights-for-the-6th-international-ion-channel-conference-ion-channel-structure-function-disease-and-therapeutics
#14
Limei Wang, Kewei Wang
To foster communication and interactions amongst international scholars and scientists in the field of ion channel research, the 6th International Ion Channel Conference (IICC-2017) was held between June 23-27, 2017 in the eastern coastal city of Qingdao, China. The meeting consisted of 450 attendees and 130 speakers and poster presenters. The program consisted of research progress, new findings and ongoing studies that were focused on (1) Ion channel structure and function; (2) Ion channel physiology and human diseases; (3) Ion channels as targets for drug discovery; (4) Technological advances in ion channel research...
November 2017: Acta Pharmaceutica Sinica. B
https://www.readbyqxmd.com/read/29149168/airway-expression-of-transient-receptor-potential-trp-vanniloid-1-and-ankyrin-1-channels-is-not-increased-in-patients-with-idiopathic-pulmonary-fibrosis
#15
Nicola-Xan Hutchinson, Allen Gibbs, Amanda Tonks, Benjamin D Hope-Gill
Dry cough is a common symptom described in patients with Idiopathic Pulmonary Fibrosis (IPF) and impairs quality of life. The exact mechanisms causing cough in IPF remain unclear, however evidence suggests altered cough neurophysiology and sensitisation plays a role; IPF patients have an enhanced cough reflex sensitivity to inhaled capsaicin. The Transient Receptor Potential Vanniloid-1 channel (TRPV-1) has a role in the cough reflex and airway expression is increased in patients with chronic cough. The Ankyrin-1 receptor (TRPA-1) is often co-expressed...
2017: PloS One
https://www.readbyqxmd.com/read/29135465/thermally-activated-trp-channels-molecular-sensors-for-temperature-detection
#16
Karen Castillo, Ignacio Diaz-Franulic, Jonathan Canan, Fernando Gonzalez-Nilo, Ramon Latorre
Temperature sensing is one of the most ancient capabilities of living organisms and is essential to sustain life, because failures in avoiding extreme noxious temperatures can result in tissue damage or death. A subset of members of the transient receptor potential (TRP) ion channel family is finely tuned to detect temperatures ranging from extreme cold to noxious heat, giving rise to thermoTRP channels. Structural and functional experiments have shown that thermoTRP channels are allosteric proteins, containing different domains that sense changes in temperature, among other stimuli, triggering pore opening...
November 14, 2017: Physical Biology
https://www.readbyqxmd.com/read/29129644/role-of-kinase-coupled-trp-channels-in-mineral-homeostasis
#17
REVIEW
Vladimir Chubanov, Lorenz Mittermeier, Thomas Gudermann
Transient receptor potential (TRP) proteins TRPM6 and TRPM7 are α-kinase-coupled divalent cation-selective channels activated upon a reduction of cytosolic levels of Mg(2+) and Mg·ATP. Emerging evidence indicate that one of the main physiological functions of TRPM6 and TRPM7 is maintaining of cellular metabolism of Mg(2+) and likely other essential metals such as Ca(2+) and Zn(2+). Recent experiments with genetic animal models have shown that TRPM6 and TRPM7 are essential for epithelial Mg(2+) transport in the placenta and intestine...
November 9, 2017: Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/29129530/glutamylation-regulates-transport-specializes-function-and-sculpts-the-structure-of-cilia
#18
Robert O'Hagan, Malan Silva, Ken C Q Nguyen, Winnie Zhang, Sebastian Bellotti, Yasmin H Ramadan, David H Hall, Maureen M Barr
Ciliary microtubules (MTs) are extensively decorated with post-translational modifications (PTMs), such as glutamylation of tubulin tails. PTMs and tubulin isotype diversity act as a "tubulin code" that regulates cytoskeletal stability and the activity of MT-associated proteins such as kinesins. We previously showed that, in C. elegans cilia, the deglutamylase CCPP-1 affects ciliary ultrastructure, localization of the TRP channel PKD-2 and the kinesin-3 KLP-6, and velocity of the kinesin-2 OSM-3/KIF17, whereas a cell-specific α-tubulin isotype regulates ciliary ultrastructure, intraflagellar transport, and ciliary functions of extracellular vesicle (EV)-releasing neurons...
November 20, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/29129206/differential-cytotoxicity-and-intracellular-calcium-signalling-following-activation-of-the-calcium-permeable-ion-channels-trpv1-and-trpa1
#19
Thomas Stueber, Mirjam J Eberhardt, Yaki Caspi, Shaya Lev, Alexander Binshtok, Andreas Leffler
Several members of the transient receptor channel (TRP) family can mediate a calcium-dependent cytotoxicity. In sensory neurons, vanilloids like capsaicin induce neurotoxicity by activating TRPV1. The closely related ion channel TRPA1 is also activated by irritants, but it is unclear if and how TRPA1 mediates cell death. In the present study we explored cytotoxicity and intracellular calcium signalling resulting from activation of TRPV1 and TRPA1, either heterologously expressed in HEK 293 cells or in native mouse dorsal root ganglion (DRG) neurons...
December 2017: Cell Calcium
https://www.readbyqxmd.com/read/29121401/mp29-02-reduces-nasal-hyperreactivity-and-nasal-mediators-in-patients-with-house-dust-mite-allergic-rhinitis
#20
Inge Kortekaas Krohn, Ina Callebaut, Yeranddy A Alpizar, Brecht Steelant, Laura Van Gerven, Per Stahl Skov, Ahmad Kasran, Karel Talavera, Mira M Wouters, Jan L Ceuppens, Sven F Seys, Peter W Hellings
BACKGROUND: Nasal hyperreactivity (NHR) is an important clinical feature of allergic rhinitis (AR). The efficacy of MP29-02 (azelastine hydrochloride (AZE) and fluticasone propionate (FP)) nasal spray on local inflammatory mediators and NHR in AR is unknown. We tested if MP29-02 decreases inflammatory mediators and NHR in AR and if this effect is due to restoration of nasal epithelial barrier function. METHODS: A 4-week double-blinded placebo-controlled trial with MP29-02 treatment was conducted in 28 patients with house dust mite (HDM) AR...
November 9, 2017: Allergy
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