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https://www.readbyqxmd.com/read/29897877/a-predicted-mannoprotein-participates-in-cryptococcus-gattii-capsular-structure
#1
Julia Catarina Vieira Reuwsaat, Heryk Motta, Ane Wichine Acosta Garcia, Carolina Bettker Vasconcelos, Bárbara Machado Marques, Natália Kronbauer Oliveira, Jéssica Rodrigues, Patrícia Aline Gröhns Ferrareze, Susana Frases, William Lopes, Vanessa Abreu Barcellos, Eamim Daidrê Squizani, Jorge André Horta, Augusto Schrank, Marcio Lourenço Rodrigues, Charley Christian Staats, Marilene Henning Vainstein, Lívia Kmetzsch
The yeast-like pathogen Cryptococcus gattii is an etiological agent of cryptococcosis. The major cryptococcal virulence factor is the polysaccharide capsule, which is composed of glucuronoxylomannan (GXM), galactoxylomannan (GalXM), and mannoproteins (MPs). The GXM and GalXM polysaccharides have been extensively characterized; however, there is little information about the role of mannoproteins in capsule assembly and their participation in yeast pathogenicity. The present study characterized the function of a predicted mannoprotein from C...
April 25, 2018: MSphere
https://www.readbyqxmd.com/read/29864585/beta-glucan-loaded-nanofiber-dressing-improves-wound-healing-in-diabetic-mice
#2
Jostein Grip, Rolf Einar Engstad, Ingrid Skjæveland, Nataša Škalko-Basnet, Johan Isaksson, Purusotam Basnet, Ann Mari Holsæter
The increased prevalence of chronic wounds requires novel treatment options. The aim of this study was to develop a beta-glucan (βG)-loaded nanofiber wound dressing. Nanofibers were prepared using the needle-free Nanospider™ technology, an electrospinning method which enables the production of nanofibers at an industrial scale. The βG was selected as active ingredient based on its confirmed wound healing potential in both animals and humans. Hydroxypropyl methylcellulose (HPMC) and polyethylene oxide (PEO) were included as copolymers...
June 1, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29859248/nutraceuticals-and-functional-foods-for-the-control-of-plasma-cholesterol-levels-an-intersociety-position-paper
#3
REVIEW
Andrea Poli, Carlo M Barbagallo, Arrigo Cicero, Alberto Corsini, Enzo Manzato, Bruno Trimarco, Franco Bernini, Francesco Visioli, Alfio Bianchi, Giuseppe Canzone, Claudio Crescini, Saula de Kreutzenberg, Nicola Ferrara, Marco Gambacciani, Andrea Ghiselli, Carla Lubrano, Giuseppe Marelli, Walter Marrocco, Vincenzo Montemurro, Damiano Parretti, Roberto Pedretti, Francesco Perticone, Roberto Stella, Franca Marangoni
Current evidence shows that cholesterol management either reduces the likelihood of cardiovascular disease (CVD) or slows down its progression. Hence, it is important that all health professionals make appropriate use of all the available intervention strategies to control risk factors: from dietary improvement and positive lifestyle changes to the use of functional foods, food supplements, and drugs. This review examines the effect of the most frequently occurring cholesterol-lowering substances in functional foods or in supplements across Europe, namely plant sterols and stanols, monacolin K found in red yeast rice, berberine and beta-glucans...
May 30, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29848566/monitoring-anti-pythium-insidiosum-igg-antibodies-and-1-3-%C3%AE-d-glucan-in-vascular-pythiosis
#4
Navaporn Worasilchai, Nitipong Permpalung, Pakawat Chongsathidkiet, Asada Leelahavanichkul, Alberto Leonel Mendoza, Tanapat Palaga, Rangsima Reantragoon, Malcolm Finkelman, Pranee Sutcharitchan, Ariya Chindamporn
Background: Despite aggressive treatment, vascular pythiosis has a mortality rate of 40%. This is due to delays in diagnosis and lack of effective monitoring tools. To overcome this drawback, serum beta-D-glucan (BG) and P. insidiosum specific antibody ( Pi -Ab) were examined as potential monitoring markers in vascular pythiosis. Methods: A prospective cohort study of vascular pythiosis patients was carried on during January 2010-July 2016. Clinical information and blood samples were collected and evaluated by the BG and Pi -Ab assays...
May 30, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29808837/effects-of-dietary-%C3%AE-glucan-supplementation-on-growth-performance-and-immunological-and-metabolic-parameters-of-weaned-pigs-administered-with-escherichia-coli-lipopolysaccharide
#5
Cheng Wu, Qin Xu, Ru Wang, Linlin Qin, Xie Peng, Liang Hu, Yan Liu, Zhengfeng Fang, Yan Lin, Shengyu Xu, Bin Feng, Jian Li, De Wu, Lianqiang Che
The objective of this study was to determine the effects of dietary β-glucan (BG) on growth performance and blood parameters in weaned pigs administered with Escherichia coli lipopolysaccharide (LPS). Twenty four pigs [24 ± 2 days old; 6.60 ± 0.04 kg body weight (BW)] were randomly allocated into two groups (12 pigs per group) with diets supplemented with 0 or BG at 200 mg kg-1 diet (CON vs. BG). These pigs were fed for a 35-day trial. On day 36, six pigs each from CON and BG were intramuscularly administered LPS (50 μg kg-1), while another 6 pigs from CON were intramuscularly administered an equivalent amount of sterile saline...
May 29, 2018: Food & Function
https://www.readbyqxmd.com/read/29755492/common-features-between-the-proteomes-of-floral-and-extrafloral-nectar-from-the-castor-plant-ricinus-communis-and-the-proteomes-of-exudates-from-carnivorous-plants
#6
Fábio C S Nogueira, Andreza R B Farias, Fabiano M Teixeira, Gilberto B Domont, Francisco A P Campos
Label-free quantitative proteome analysis of extrafloral (EFN) and floral nectar (FN) from castor ( Ricinus communis ) plants resulted in the identification of 72 and 37 proteins, respectively. Thirty proteins were differentially accumulated between EFN and FN, and 24 of these were more abundant in the EFN. In addition to proteins involved in maintaining the nectar pathogen free such as chitinases and glucan 1,3-beta-glucosidase, both proteomes share an array of peptidases, lipases, carbohydrases, and nucleases...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29734706/phenotypic-and-physiological-evaluation-of-two-and-six-rows-barley-under-different-environmental-conditions
#7
Mahmoud Naser, Mohamed Badran, Hanaa Abouzied, Heba Ali, Ibrahim Elbasyoni
In recent years, barley has attracted more interest as a food and feed source because of its high soluble dietary fiber and β-glucan content compared with other small grains. Twenty-five barley genotypes (20 imported genotypes and five check cultivars) were grown in three environments for two successive seasons: 2015/2016 and 2016/2017. The first environment was in El-Nubaria, Alexandria, Egypt during 2015/2016, while the second and third environments were in El-Bostan, Elbhera, Egypt during 2015/2016 and 2016/2017...
May 4, 2018: Plants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29724391/the-association-between-endotoxin-and-beta-1-%C3%A2-3-d-glucan-in-house-dust-with-asthma-severity-among-schoolchildren
#8
Oluwafemi Oluwole, Donna C Rennie, Ambikaipakan Senthilselvan, Roland Dyck, Anna Afanasieva, Shelley Kirychuk, George Katselis, Joshua A Lawson
BACKGROUND: Asthma severity can be affected by microbial exposures. However, less is known about the specific indoor agents aggravating the disease in children. We examined the associations between indoor endotoxin and beta-(1 → 3)-D-glucan exposures and asthma severity in children with asthma. METHODS: A clinical cross-sectional study of schoolchildren (aged 7-17 years) was conducted in the province of Saskatchewan, Canada. Children with asthma (n = 116) were identified from 335 participants using a combination of survey responses and objective clinical assessments...
May 2018: Respiratory Medicine
https://www.readbyqxmd.com/read/29710434/improved-hcp-reduction-using-a-new-all-synthetic-depth-filtration-media-within-an-antibody-purification-process
#9
Hoang C Nguyen, Amie L Langland, John P Amara, Michael Dullen, David S Kahn, Joseph A Costanzo
Biologic manufacturing processes typically employ clarification technologies like depth filtration to remove insoluble and soluble impurities. Conventional depth filtration media used in these processes contain naturally-derived components like diatomaceous earth and cellulose. These components may introduce performance variability and contribute extractable/leachable components like beta-glucans that could interfere with limulus amebocyte lysate endotoxin assays. Recently a novel, all-synthetic depth filtration media is developed (Millistak+® HC Pro X0SP) that may improve process consistency, efficiency, and drug substance product quality by reducing soluble process impurities...
April 30, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29684456/radioprotective-effect-of-orally-administered-beta-d-glucan-derived-from-saccharomyces-cerevisiae
#10
Fang Liu, Zhuanzi Wang, Jia Liu, Wenjian Li
The present study was to evaluate the in vivo radioprotective effect of oral administration of Saccharomyces cerevisiae-derived-beta-d-glucan (S. cerevisiae-BG) and to investigate the protective mechanism. The results demonstrated that oral pretreatment with 350 mg/kg S. cerevisiae-BG once daily for 14 consecutive days significantly increased the survival rate of mice from 6 Gy X-rays irradiation. At the 30th day after irradiation, cellularity and the percentage of hematopoietic stem/progenitor cells in bone marrow (BM) of surviving mice were increased by S...
April 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29655683/bronchodilator-effects-of-lignosus-rhinocerotis-extract-on-rat-isolated-airways-is-linked-to-the-blockage-of-calcium-entry
#11
Mei-Kee Lee, Kuan-Hon Lim, Paul Millns, Suresh Kumar Mohankumar, Szu-Ting Ng, Chon-Seng Tan, Sue-Mian Then, Yvonne Mbaki, Kang-Nee Ting
BACKGROUND: Lignosus rhinocerotis (Cooke) Ryvarden is a popular medicinal mushroom used for centuries in Southeast Asia to treat asthma and chronic cough. The present study aimed to investigate the effect of this mushroom on airways patency. MATERIALS AND METHODS: The composition of L. rhinocerotis TM02 cultivar was analyzed. Organ bath experiment was employed to study the bronchodilator effect of Lignosus rhinocerotis cold water extract (CWE) on rat isolated airways...
March 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29648596/unraveling-synthesis-of-the-cryptococcal-cell-wall-and-capsule
#12
Zhuo A Wang, Lucy X Li, Tamara L Doering
Fungal pathogens cause devastating infections in millions of individuals each year, representing a huge but underappreciated burden on human health. One of these, the opportunistic fungus Cryptococcus neoformans, kills hundreds of thousands of patients annually, disproportionately affecting people in resource-limited areas. This yeast is distinguished from other pathogenic fungi by a polysaccharide capsule that is displayed on the cell surface. The capsule consists of two complex polysaccharide polymers: a mannan substituted with xylose and glucuronic acid, and a galactan with galactomannan side chains that bear variable amounts of glucuronic acid and xylose...
April 10, 2018: Glycobiology
https://www.readbyqxmd.com/read/29604620/supplementation-of-caricol%C3%A2-gastro-reduces-chronic-gastritis-disease-associated-pain
#13
Friedrich-Anton Weiser, Michael Fangl, Wilhelm Mosgoeller
OBJECTIVE: Papaya and oats are natural food and used in traditional medicine in many parts of the world. Papaya has a high content of enzymes supporting digestive function. Oats are a source of minerals, beta-glucan fibres, immunmodulatory and antiinflammatory probiotic substances. Caricol®-Gastro combines both constituents, it was designed as vegan organic preparation for intestinal inflammatory diseases. We performed a randomized, double blind placebo controlled clinical trial to investigate the potential of Caricol®-Gastro as add on therapy in patients with diagnosed chronic gastritis...
March 27, 2018: Neuro Endocrinology Letters
https://www.readbyqxmd.com/read/29579935/characterization-of-oat-beta-glucan-and-coenzyme-q10-loaded-beta-glucan-powders-generated-by-the-pressurized-gas-expanded-liquid-pgx-technology
#14
Nian Liu, Ricardo Couto, Bernhard Seifried, Paul Moquin, Luis Delgado, Feral Temelli
The physicochemical properties of the oat beta-glucan powder (BG) and coenzyme Q10 (CoQ10)-loaded BG powder (L-BG) produced by the pressurized gas-expanded liquid (PGX) technology were studied. Helium ion microscope, differential scanning calorimeter, X-ray diffractometer, AutoSorb iQ and rheometer were used to determine the particle morphology, thermal properties, crystallinity, surface area and viscosity, respectively. Both BG (7.7μm) and L-BG (6.1μm) were produced as micrometer-scale particles, while CoQ10 nanoparticles (92nm) were adsorbed on the porous structure of L-BG...
April 2018: Food Research International
https://www.readbyqxmd.com/read/29528125/development-of-an-orange-juice-beverage-formulated-with-oat-beta-glucan-and-whey-protein-isolate
#15
Wenjing Wan, Baojun Xu
BACKGROUND: A beverage was developed using beta-glucan and whey protein isolate (WPI). The sensory attributes and changes of the physiochemical properties (pH value, total acidity, total soluble solids, sugar-acid ratio, color, viscosity, turbidity, and browning index) of the beverage during 48-h storage were evaluated by comparison with the commercial product and a blank sample. RESULTS: The sweetness, smoothness, and overall acceptance of the developed beverage were similar to the commercial and blank samples...
March 12, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29524446/beta-glucan-enhances-the-response-to-svcv-infection-in-zebrafish
#16
Regla M Medina-Gali, María Del Mar Ortega-Villaizan, Luis Mercado, Beatriz Novoa, Julio Coll, Luis Perez
The antiviral effects of beta-glucan, an immunostimulatory agent were studied in zebrafish both in vitro and in vivo. Here we show that zebrafish ZF4 cells as well as whole fish primed with yeast β-glucan zymosan exhibited increased cytokine expression and elevated response to spring viremia of carp virus (SVCV) infection. In vitro, previous treatment of β-glucan enhanced ZF4 cell viability against SVCV infection which is associated to the activation of interferon signaling pathway and inflammatory cytokines gene expression...
July 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29486797/a-randomized-controlled-trial-evaluating-the-efficacy-and-safety-of-bth1677-in-combination-with-bevacizumab-carboplatin-and-paclitaxel-in-first-line-treatment-of-advanced-non-small-cell-lung-cancer
#17
Walburga Engel-Riedel, Jamie Lowe, Paulette Mattson, J Richard Trout, Richard D Huhn, Michele Gargano, Myra L Patchen, Richard Walsh, My My Trinh, Mariève Dupuis, Folker Schneller
BACKGROUND: BTH1677, a beta-glucan pathogen-associated molecular pattern molecule, drives an anti-cancer immune response in combination with oncology antibody therapies. This phase II study explored the efficacy, pharmacokinetics (PK), and safety of BTH1677 combined with bevacizumab/carboplatin/paclitaxel in patients with untreated advanced non-small cell lung cancer (NSCLC). METHODS: Patients were randomized to the BTH1677 arm (N = 61; intravenous [IV] BTH1677, 4 mg/kg, weekly; IV bevacizumab, 15 mg/kg, once each 3-week cycle [Q3W]; IV carboplatin, 6 mg/mL/min Calvert formula area-under-the-curve, Q3W; and IV paclitaxel, 200 mg/m2 , Q3W) or Control arm (N = 31; bevacizumab/carboplatin/paclitaxel as above)...
February 27, 2018: Journal for Immunotherapy of Cancer
https://www.readbyqxmd.com/read/29485753/a-novel-meloidogyne-graminicola-effector-mgmo237-interacts-with-multiple-host-defence-related-proteins-to-manipulate-plant-basal-immunity-and-promote-parasitism
#18
Jiansong Chen, Lili Hu, Longhua Sun, Borong Lin, Kun Huang, Kan Zhuo, Jinling Liao
Plant-parasitic nematodes can secrete effector proteins into the host tissue to facilitate their parasitism. In this study, we report a novel effector protein, MgMO237, from Meloidogyne graminicola, which is exclusively expressed within the dorsal oesophageal gland cell and markedly up-regulated in parasitic third-/fourth-stage juveniles of M. graminicola. Transient expression of MgMO237 in protoplasts from rice roots showed that MgMO237 was localized in the cytoplasm and nucleus of the host cells. Rice plants overexpressing MgMO237 showed an increased susceptibility to M...
February 27, 2018: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29467823/in-vitro-and-in-vivo-characterization-of-three-cellvibrio-japonicus-glycoside-hydrolase-family-5-members-reveals-potent-xyloglucan-backbone-cleaving-functions
#19
Mohamed A Attia, Cassandra E Nelson, Wendy A Offen, Namrata Jain, Gideon J Davies, Jeffrey G Gardner, Harry Brumer
Background: Xyloglucan (XyG) is a ubiquitous and fundamental polysaccharide of plant cell walls. Due to its structural complexity, XyG requires a combination of backbone-cleaving and sidechain-debranching enzymes for complete deconstruction into its component monosaccharides. The soil saprophyte Cellvibrio japonicus has emerged as a genetically tractable model system to study biomass saccharification, in part due to its innate capacity to utilize a wide range of plant polysaccharides for growth...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29435400/encapsulation-of-cadmium-selenide-nanocrystals-in-biocompatible-nanotubes-dft-calculations-x-ray-diffraction-investigations-and-confocal-fluorescence-imaging
#20
David G Calatayud, Haobo Ge, Navaratnarajah Kuganathan, Vincenzo Mirabello, Robert M J Jacobs, Nicholas H Rees, Craig T Stoppiello, Andrei N Khlobystov, Rex M Tyrrell, Enrico Da Como, Sofia I Pascu
The encapsulation of CdSe nanocrystals within single-walled carbon nanotube (SWNT) cavities of varying dimensions at elevated temperatures under strictly air-tight conditions is described for the first time. The structures of CdSe nanocrystals under confinement inside SWNTs was established in a comprehensive study, combining both experimental and DFT theoretical investigations. The calculated binding energies show that all considered polymorphs [(3:3), (4:4), and (4:2)] may be obtained experimentally. The most thermodynamically stable structure (3:3) is directly compared to the experimentally observed CdSe structures inside carbon nanotubes...
February 2018: ChemistryOpen
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