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Pbmc treated with alcohol

Yusuke Tsuchimoto, Akira Asai, Yasuhiro Tsuda, Ichiaki Ito, Tomoki Nishiguchi, Melanie C Garcia, Sumihiro Suzuki, Makiko Kobayashi, Kazuhide Higuchi, Fujio Suzuki
Chronic alcohol consumption markedly impairs host antibacterial defense against opportunistic infections. γ-irradiated NOD-SCID IL-2Rγ(null) mice inoculated with nonalcoholic PBMCs (control PBMC chimeras) resisted Klebsiella pneumonia and gut bacteria-associated sepsis, whereas the chimeras created with alcoholic PBMCs (alcoholic PBMC chimeras) were very susceptible to these infections. M1 monocytes (IL-12(+)IL-10(-)CD163(-)CD14(+) cells), major effector cells in antibacterial innate immunity, were not induced by a bacterial Ag in alcoholic PBMC cultures, and M2b monocytes (CCL1(+)CD163(+)CD14(+) cells), which predominated in alcoholic PBMCs, were shown to be inhibitor cells on the Ag-stimulated monocyte conversion from quiescent monocytes to M1 monocytes...
December 1, 2015: Journal of Immunology: Official Journal of the American Association of Immunologists
Egeria Scoditti, Alessia Nestola, Marika Massaro, Nadia Calabriso, Carlo Storelli, Raffaele De Caterina, Maria Annunziata Carluccio
OBJECTIVE: Hydroxytyrosol (HT), the major olive oil antioxidant polyphenol in cardioprotective Mediterranean diets, is endowed with anti-inflammatory and anti-atherosclerotic activity. The production of cyclooxygenase (COX)-2-dependent inflammatory eicosanoids and the functionally linked release of matrix metalloproteinase (MMP)-9 by macrophages likely contribute to plaque instability leading to acute coronary events. Objective of the study was to examine the HT effects on inflammatory markers in human activated monocytes, including MMP-9 and COX-2 activity and expression and explore HT underlying mechanisms...
January 2014: Atherosclerosis
Kalaiselvan Ilavarasi, Perumal Vijayaraman Kiruthiga, Shunmugiah Karutha Pandian, Kasi Pandima Devi
The protective effect of hydroxytyrosol (HT), a strong antioxidant compound from extra virgin olive oil, against TCDD induced toxicity was investigated in human peripheral blood mononuclear cells (PBMC). PBMC (1 × 10(6)cellsmL(-1)) were divided into four groups and were incubated in a CO(2) incubator (5% CO(2)) for 12h with vehicle, TCDD (10 nM), TCDD+HT (10 nM+100 μM) and HT alone (100 μM) respectively. To clarify the role of HT against TCDD induced cytotoxicity, oxidative stress and the levels of antioxidant enzymes were assessed...
August 2011: Chemosphere
A J di Mambro, R Parker, A McCune, F Gordon, C M Dayan, P Collins
UNLABELLED: Steroids improve the outcome in alcoholic hepatitis (AH), but up to 40% of patients fail to respond adequately. Interleukin-2 (IL-2) exacerbates steroid resistance in vitro. We performed a prospective study to determine if intrinsic steroid sensitivity correlates with response to steroids in individuals with severe AH and if IL-2 receptor blockade can reverse this. Peripheral blood mononuclear cells (PBMCs) were isolated from 20 patients with AH and a Maddrey's score >32...
April 2011: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Nadka I Boyadjieva, María Ortigüela, Alvaro Arjona, Xiaodong Cheng, Dipak K Sarkar
BACKGROUND: Natural killer (NK) cell dysfunction is associated with hyperresponse of corticotropin releasing hormone (CRH) to immune challenge and with a loss of beta-endorphin (BEP) neurons in fetal alcohol exposed animals. Recently, we established a method to differentiate neural stem cells into BEP neurons using cyclic adenosine monophosphate (cAMP)-elevating agents in cultures. Hence, we determined whether in vitro differentiated BEP neurons could be used for reversing the compromised stress response and immune function in fetal alcohol exposed rats...
May 2009: Alcoholism, Clinical and Experimental Research
Yang-Ming Tseng, Sheng-Yi Chen, Chien-Hung Chen, Yi-Ru Jin, Shih-Meng Tsai, Ing-Jun Chen, Jang-Hwa Lee, Chzng-Cheng Chiu, Li-Yu Tsai
Excessive alcohol consumption can induce apoptosis in a variety of tissues and influence the antioxidant status in peripheral blood mononuclear cells (PBMC). This paper investigates the effects of whey protein concentrate (WPC) pretreated in PBMC on the apoptosis and antioxidant status after the treatment of alcohol. The results show that the percentages of apoptotic cells in the alcohol-treated group were higher than those in the group without alcohol treatment. Additionally, there was higher glutathione (GSH) peroxidase (GPx) activity when the PBMC were treated with 300 mg/dL of alcohol...
September 10, 2008: Journal of Agricultural and Food Chemistry
Dustin A McCann, Avery Solco, Yi Liu, Filippo Macaluso, Patricia A Murphy, Marian L Kohut, David S Senchina
Echinacea spp. phytomedicines are popular for treating upper respiratory infections. The purpose of this investigation was to examine the immunomodulatory properties of Echinacea tinctures from seven species after being stored at -20 degrees C for 2 years. Two experimental techniques were employed using human peripheral blood mononuclear cells (PBMC). In the first set of experiments, PBMCs were stimulated in vitro with tinctures alone and assayed for proliferation and production of interleukin-10 (IL-10), IL-12, and tumor necrosis factor-alpha (TNF-alpha)...
May 2007: Journal of Interferon & Cytokine Research
Chuan-Chen Ho, Shih-Shen Lin, Ming-Yung Chou, Fang-Lung Chen, Chao-Chin Hu, Chung-Shih Chen, Guan-Yu Lu, Chi-Chiang Yang
OBJECTIVES: Five CAPE-like compounds, namely caffeic acid phenethyl ester (CAPE), methyl caffeate (MC), ethyl 3-(3,4-dihydroxyphenyl)acrylate (EC), phenethyl dimethyl caffeate (PEDMC) and phenethyl 3-(4-bromophenyl)acrylic (BrCAPE) were tested for their anti-HIV replication in vitro and immune modulation effects in vivo. METHODS: Short-term cytotoxicity was assessed by Trypan Blue stain and MTT assay. For antiviral assays, M-tropic (strain JRCSF), T-tropic (strain NL-4-3) and dual tropic (strain 89...
August 2005: Journal of Antimicrobial Chemotherapy
Giovanni Pertosa, Simona Simone, Michela Soccio, Daniela Marrone, Loreto Gesualdo, Francesco Paolo Schena, Giuseppe Grandaliano
Priming of the coagulation cascade during hemodialysis (HD) leads to the release of activated factor X (FXa). The binding of FXa to its specific receptors, effector protease receptor-1 (EPR-1) and protease-activated receptor-2 (PAR-2), may induce the activation of peripheral blood mononuclear cells (PBMC) and promote a chronic inflammatory state that is responsible for several HD-related morbidities. In the attempt to elucidate the mechanisms underlying the coagulation-associated inflammation in HD, 10 HD patients were randomized to be treated subsequently with a cellulose acetate membrane (CA) and Ethylen-vinyl-alcohol (EVAL), a synthetic membrane that has been shown to reduce FXa generation...
August 2005: Journal of the American Society of Nephrology: JASN
K B Massirer, M H Hirata, A E B Silva, M L G Ferraz, N Y Nguyen, R D C Hirata
Interferon (IFN)-alpha receptor mRNA expression in liver of patients with chronic hepatitis C has been shown to be a response to IFN-alpha therapy. The objective of the present study was to determine whether the expression of mRNA for subunit 1 of the IFN-alpha receptor (IFNAR1) in peripheral blood mononuclear cells (PBMC) is associated with the response to IFN-alpha in patients with chronic hepatitis C. Thirty patients with positive anti-HCV and HCV-RNA, and abnormal levels of alanine aminotransferase in serum were selected and treated with IFN-alpha 2b for one year...
May 2004: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
P Fanti, M C Monier-Faugere, Z Geng, D Cohen, H H Malluche
Epidemiological studies suggest that moderate consumption of alcoholic beverages may be beneficial for bone in postmenopausal women. To investigate prospectively these uncontrolled observations, female rats were divided in four groups of 10 animals each and treated with 1) ovariectomy (OVX) and 2.5% ethanol diet (OVX-ETOH group), 2) OVX and control diet (OVX-C group), 3) sham surgery and 2.5% ethanol diet (SHAM-ETOH group), or 3) sham surgery and control diet (SHAM-C group). Three weeks after surgery, bone histomorphometry revealed that the OVX-C group, as expected, had lower trabecular bone volume and higher parameters of bone formation and resorption than the SHAM-C group (p < 0...
September 1997: Alcoholism, Clinical and Experimental Research
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