Mikhail Melnik, Emily Miyoshi, Ricky Ma, Maria Corrada, Claudia Kawas, Ryan Bohannan, Chad Caraway, Carol A Miller, Jason D Hinman, Varghese John, Tina Bilousova, Karen H Gylys
BACKGROUND: The neuronal and gliaI populations within the brain are tightly interwoven, making isolation and study of large populations of a single cell type from brain tissue a major technical challenge. Concurrently, cell-type specific extracellular vesicles (EVs) hold enormous diagnostic and therapeutic potential in neurodegenerative disorders including Alzheimer's disease (AD). NEW METHOD: Postmortem AD cortical samples were thawed and gently dissociated. Following filtration, myelin and red blood cell removal, cell pellets were immunolabeled with fluorescent antibodies and analyzed by flow cytometry...
April 14, 2024: Journal of Neuroscience Methods